DANIEL FILIPE MESTRE SIMÃO (0A15-08E9-448D)

Curriculum Vitae

DANIEL FILIPE MESTRE SIMÃO

Daniel Simão is a Lab Head at the Animal Cell Technology Unit of IBET's Health & Pharma Division. D. Simão graduated in Biology (University of Lisbon) and holds an MSc in Molecular Genetics and Biomedicine (NOVA School of Science and Technology). Obtained his PhD in Bioengineering Systems from the MIT-Portugal Doctoral Program in 2016. Having pursued his PhD studies in the Advanced Cell Models Laboratory (iBET and ITQB NOVA), D. Simão focused mostly on the development of stem cell-based 3D in vitro models of central nervous system for preclinical research. His work contributed for the establishment of neural differentiation methodologies based on stirred culture systems, interrogating functional aspects of neuron-glia interactions and applying such models for evaluation of viral vectors for gene therapy. Joined the Adenovirus Receptors, Trafficking and Vectorology Laboratory at IGMM (Montpellier, France) for a post-doc, working on the construction and production of CAV-2 vectors for gene transfer to central nervous system. In 2017, Daniel became Senior Researcher at iBET responsible for the development of a microfluidics-based antibody discovery platform. As Lab Head since 2021, Daniel coordinates a team working on R&D projects for biopharmaceutical industry, focusing on biologics research. D. Simão has authored 18 manuscripts, 3 conference papers and 1 book chapter; Has received 5 awards and/or honors and participated as speaker in 11 national and international conferences. Daniel has been responsible for the co-supervision of one PhD Student and has participated as invited lecturer in Master and PhD programs (NOVA Medical School and ITQB/iBET). Was the Principal Investigator of the FCT funded project AstroReact and the internally-funded (iBET) project FibroTag, in the fields of development of human cell models for preclinical research and antibody discovery; Participated as team member in several other publicly funded research projects (FCT and EC), and operates as project manager in research services with the biopharmaceutical industry.

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Identificação

Identificação pessoal

Nome completo: DANIEL FILIPE MESTRE SIMÃO

Nomes de citação

Simão, Daniel

Identificadores de autor

Ciência ID: 0A15-08E9-448D
ORCID iD: 0000-0002-7532-8081
Researcher Id: AAF-2747-2021

Moradas

Avenida da República, Estação Agronómica Nacional, Edifício iBET/ITQB-NOVA, 2780-157, Oeiras, Oeiras, Portugal (Profissional)

Websites

https://www.linkedin.com/in/daniel-sim%C3%A3o-490a5252/ (Rede social)

Domínios de atuação

Ciências Médicas e da Saúde - Biotecnologia Médica - Tecnologias que envolvem a Manipulação de Células,Tecidos,Órgãos ou todo o Organismo
Ciências Naturais - Ciências Biológicas - Biologia Celular
Ciências Naturais - Ciências Biológicas - Biologia Molecular

Idiomas

Idioma	Conversação	Leitura	Escrita	Compreensão
Português (Idioma materno)
Inglês	Utilizador proficiente (C2)	Utilizador proficiente (C2)	Utilizador proficiente (C2)	Utilizador proficiente (C2)
Espanhol; Castelhano	Utilizador elementar (A1)	Utilizador independente (B1)	Utilizador elementar (A1)	Utilizador elementar (A1)

Formação

	Grau	Classificação
2016 Concluído	Bioengineering Systems (MIT-Portugal) (Doutoramento) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal "Development of human central nervous system 3D in vitro models for preclinical research" (TESE/DISSERTAÇÃO)	Aprovado, por unanimidade
2010 Concluído	Mestrado em Genética Molecular e Biomedicina (Mestrado) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal "Novas Linhas Celulares Humanas para Produção de Adenovírus com Aplicação em Terapia Génica" (TESE/DISSERTAÇÃO)	18
2008 Concluído	Biologia - Ramo de Biologia Molecular e Genética (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal	15

Percurso profissional

Ciência

	Categoria Profissional Instituição de acolhimento	Empregador
2021/02/01 - Atual	Investigador principal (carreira) (Investigação)	Instituto de Biologia Experimental e Tecnológica, Portugal
	Instituto de Biologia Experimental e Tecnológica, Portugal
2017/07/01 - 2021/01/31	Investigador Contratado (Investigação)	Instituto de Biologia Experimental e Tecnológica, Portugal

2016/09/01 - 2017/06/30	Pós-doutorado (Investigação)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

2016/04/01 - 2016/08/31	Investigador Contratado (Investigação)	Institut de Génétique Moléculaire Montpellier, França

2016/01/01 - 2016/03/31	Investigador (Investigação)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

2012/01/01 - 2015/12/31	Investigador (Investigação)	Fundação para a Ciência e a Tecnologia, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2010/03/16 - 2011/09/30	Assistente de Investigação (carreira) (Investigação)	Instituto de Biologia Experimental e Tecnológica, Portugal

Outros

	Categoria Profissional Instituição de acolhimento	Empregador
2014/01 - 2014/02	Visiting PhD Student	Norges Teknisk-Naturvitenskapelige Universitet Fakultet for medisin og helsevitenskap, Noruega

Projetos

Bolsa

	Designação	Financiadores
2019/01/01 - 2019/10/31	iNOVA4Health - Programme in Translational Medicine (iBET, CEDOC/FCM, IPOLFG and ITQB) UID/Multi/04462/2019 Instituto Português de Oncologia de Lisboa Francisco Gentil EPE, Portugal Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal Universidade Nova de Lisboa, Portugal Universidade Nova de Lisboa Centro de Estudos de Doenças Crónicas, Portugal Universidade Nova de Lisboa Medical School, Portugal Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluído

Projeto

	Designação	Financiadores
2023/09/01 - Atual	Life Sciences for a Healthy and Sustainable Future LA/P/0087/2020 10.54499/LA/P/0087/2020 Instituto Português de Oncologia de Lisboa Francisco Gentil EPE, Portugal Universidade Nova de Lisboa Molecular Structural and Cellular Microbiology, Portugal Universidade Nova de Lisboa, Portugal Instituto de Biologia Experimental e Tecnológica, Portugal Instituto Gulbenkian de Ciência, Portugal Universidade de Lisboa Centro de Estudos Comparatistas, Portugal BioResources 4 Sustainability, Portugal Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal	Fundação para a Ciência e a Tecnologia Em curso
2020/06/01 - 2022/05/31	Novel antibody-based tools for targeting cardiac fibrosis iBETxplore_2019_738 Investigador responsável Instituto de Biologia Experimental e Tecnológica, Portugal	Instituto de Biologia Experimental e Tecnológica Em curso
2018/10/01 - 2021/09/30	Impacto das alterações do microambiente neural induzidas por astrócitos na patobiologia de lesões traumáticas cerebrais PTDC/BTM-ORG/29580/2017 Investigador responsável Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Em curso
2014/12/01 - 2018/05/31	CARDIOSTEM: Tecidos cardíacos e terapias baseadas em células estaminais para aplicações cardiovasculares MITP-TB/ECE/0013/2013 Bolseiro de Investigação Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal Centro Hospitalar de Lisboa Central EPE, Portugal Instituto de Biologia Experimental e Tecnológica, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Concluído
2012/03 - 2015/08	Human central nervous system in vitro models for preclinical research: new tools for studying viral vector-mediated gene delivery in 3D cell context Provided by PTCRIS: 119243 Bolseiro de Investigação	Fundação para a Ciência e a Tecnologia, I.P.
2014/04/01 - 2015/03/31	Microscopia microfluídica de "light-sheet" para characterização de modelos celulares tridimensionais e peixa-zebra EXPL/BBB-IMG/0363/2013 Fundação Champalimaud, Portugal Fundação Calouste Gulbenkian, Portugal Instituto Gulbenkian de Ciência, Portugal Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluído
2014/04 - 2015/03	Microfluidics light-sheet microscopy for increased throughput analysis of 3D cell models and zebra-fish Provided by PTCRIS: 133385 Bolseiro de Investigação	Fundação para a Ciência e a Tecnologia, I.P.

Produções

Publicações

Artigo em conferência	Simão, Daniel. "Development of an anti-HER2 phage to monitor untargeted cell pannings". Trabalho apresentado em 26th European Society for Animal Cell Technology Meeting - Cell culture technologies: bridging academia and industry to provide solutions for patients, 2019. Simão D; Pinto C; Teixeira AP; Alves PM; Brito C. "1H-NMR spectroscopy for human 3D neural stem cell cultures metabolic profiling.". Trabalho apresentado em 3rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, 2013. 10.1186/1753-6561-7-s6-o8 Simão D; Costa I; Serra M; Schwarz J; Brito C; Alves PM. "Towards human central nervous system in vitro models for preclinical research: strategies for 3D neural cell culture.". Trabalho apresentado em 22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies, 2011. 10.1186/1753-6561-5-S8-P53
Artigo em revista	Catarina M. Gomes; Maria João Sebastião; Gabriela Silva; Filipa Moura; Daniel Simão; Patrícia Gomes-Alves; Paula M. Alves; Catarina Brito. "Miniaturization of hiPSC-derived 3D neural cultures in stirred-tank bioreactors for parallelized preclinical assessment of rAAV". Frontiers in Bioengineering and Biotechnology (2024): http://dx.doi.org/10.3389/fbioe.2024.1379597. 10.3389/fbioe.2024.1379597 Inês C. Ferreira; Estefania Torrejón; Bernardo Abecasis; Bruno M. Alexandre; Ricardo A. Gomes; Chris Verslype; Jos van Pelt; et al. "Aldehyde Dehydrogenase 2 (ALDH2): A novel sorafenib target in hepatocellular carcinoma unraveled by the proteome-wide cellular thermal shift assay". SLAS Discovery (2024): https://doi.org/10.1016/j.slasd.2024.100154. 10.1016/j.slasd.2024.100154 Gabriela Silva; Ana F. Rodrigues; Susana Ferreira; Carolina Matos; Rute P. Eleutério; Gonçalo Marques; Khrystyna Kucheryava; et al. "Novel scFv against Notch Ligand JAG1 Suitable for Development of Cell Therapies toward JAG1-Positive Tumors". Biomolecules (2023): https://www.mdpi.com/2218-273X/13/3/459. 10.3390/biom13030459 Moita MR; Silva MM; Diniz C; Serra M; Hoet RM; Barbas A; Simão D. "Transcriptome and proteome profiling of activated cardiac fibroblasts supports target prioritization in cardiac fibrosis.". Frontiers in cardiovascular medicine (2022): http://europepmc.org/abstract/med/36531712. 10.3389/fcvm.2022.1015473 Simão D; Gomes CM; Alves PM; Brito C. "Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models.". Biotechnology advances (2021): http://europepmc.org/abstract/med/34875362. 10.1016/j.biotechadv.2021.107883 Vasconcelos E Sá J; Simão D; Terrasso AP; Silva MM; Brito C; Isidro IA; Alves PM; Carrondo MJT. "Unveiling dynamic metabolic signatures in human induced pluripotent and neural stem cells.". PLoS computational biology (2020): http://europepmc.org/abstract/med/32298259. 10.1371/journal.pcbi.1007780 Arez F; Rebelo SP; Fontinha D; Simão D; Martins TR; Machado M; Fischli C; et al. "Flexible 3D Cell-Based Platforms for the Discovery and Profiling of Novel Drugs Targeting Plasmodium Hepatic Infection.". ACS infectious diseases (2019): http://europepmc.org/abstract/med/31479238. 10.1021/acsinfecdis.9b00144 Bayó-Puxan N; Terrasso AP; Creyssels S; Simão D; Begon-Pescia C; Lavigne M; Salinas S; et al. "Lysosomal and network alterations in human mucopolysaccharidosis type VII iPSC-derived neurons.". Scientific reports (2018): http://europepmc.org/abstract/med/30413728. 10.1038/s41598-018-34523-3 Silva MM; Gomes-Alves P; Rosa S; Simão D; Inácio JM; Peixoto C; Serra M; Belo JA; Alves PM. "Full-length human CCBE1 production and purification: leveraging bioprocess development for high quality glycosylation attributes and functionality.". Journal of biotechnology (2018): http://europepmc.org/abstract/med/30165116. 10.1016/j.jbiotec.2018.08.015 Simão D; Silva MM; Terrasso AP; Arez F; Sousa MFQ; Mehrjardi NZ; Šaric T; et al. "Recapitulation of Human Neural Microenvironment Signatures in iPSC-Derived NPC 3D Differentiation.". Stem cell reports (2018): http://europepmc.org/abstract/med/30057262. 10.1016/j.stemcr.2018.06.020 Simão D; Pinto C; Fernandes P; Peddie CJ; Piersanti S; Collinson LM; Salinas S; et al. "Evaluation of helper-dependent canine adenovirus vectors in a 3D human CNS model.". (2016): http://europepmc.org/abstract/med/26181626. 10.1038/gt.2015.75 Simão D; Terrasso AP; Teixeira AP; Brito C; Sonnewald U; Alves PM. "Functional metabolic interactions of human neuron-astrocyte 3D in vitro networks.". (2016): http://europepmc.org/abstract/med/27619889. 10.1038/srep33285 Silva AC; Simão D; Küppers C; Lucas T; Sousa MF; Cruz P; Carrondo MJ; Kochanek S; Alves PM. "Human amniocyte-derived cells are a promising cell host for adenoviral vector production under serum-free conditions.". (2015): http://europepmc.org/abstract/med/25943527. 10.1002/biot.201400765 Simão D; Pinto C; Piersanti S; Weston A; Peddie CJ; Bastos AE; Licursi V; et al. "Modeling human neural functionality in vitro: three-dimensional culture for dopaminergic differentiation.". (2015): http://europepmc.org/abstract/med/25257211. 10.1089/ten.tea.2014.0079 Fernandes P; Simão D; Guerreiro MR; Kremer EJ; Coroadinha AS; Alves PM. "Impact of adenovirus life cycle progression on the generation of canine helper-dependent vectors.". (2015): http://europepmc.org/abstract/med/25338917. 10.1038/gt.2014.92 Piersanti S; Burla R; Licursi V; Brito C; La Torre M; Alves PM; Simao D; et al. "Transcriptional Response of Human Neurospheres to Helper-Dependent CAV-2 Vectors Involves the Modulation of DNA Damage Response, Microtubule and Centromere Gene Groups.". (2015): http://europepmc.org/abstract/med/26207738. 10.1371/journal.pone.0133607 Gualda EJ; Simão D; Pinto C; Alves PM; Brito C. "Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy.". (2014): http://europepmc.org/abstract/med/25161607. 10.3389/fncel.2014.00221 Brito C; Simão D; Costa I; Malpique R; Pereira CI; Fernandes P; Serra M; et al. "3D cultures of human neural progenitor cells: dopaminergic differentiation and genetic modification. [corrected].". (2012): http://europepmc.org/abstract/med/22433395. 10.1016/j.ymeth.2012.03.005 Brito, C.; Simão, D.; Costa, I.; Malpique, R.; Pereira, C.I.; Fernandes, P.; Serra, M.; et al. "Corrigendum to " Generation and genetic modification of 3D cultures of human dopaminergic neurons derived from neural progenitor cells" [Methods 56 (2012) 452-460]". Methods 57 1 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84865089501&partnerID=MN8TOARS. 10.1016/j.ymeth.2012.07.014
Capítulo de livro	Simão D; Arez F; Terasso AP; Pinto C; Sousa MF; Brito C; Alves PM. "Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells.". In Bioreactors in Stem Cell Biology. New York, Estados Unidos: Humana Press, 2016. Publicado • 10.1007/7651_2016_333

Atividades

Apresentação oral de trabalho

	Título da apresentação	Nome do evento Anfitrião (Local do evento)
2023	In Vitro Tools to Explore Cardiac Fibrosis Target Space	Antibody Engineering & Therapeutics Europe (Amsterdam, Países Baixos)
2021/03/08	Human Stem Cells in Disease Modelling and Drug Discovery	VI Semana da Bioengenharia Instituto Superior Técnico (Lisbon, Portugal)
2019/04/19	Mimicking disease microenvironments in Bioreactors	3D Cell Cultures and Organ-on-a-Chip in the Pharmaceutical Toxicology and Pathology - Use and Applicability Dutch Society of Toxicology (NVT) (Leiden, Países Baixos)
2019/04/05	Recapitulation of Human Neural Microenvironment Signatures in iPSC-derived NPC 3D Differentiation	Advancing Biologics Characterization by Mass Spectrometry Seminar iBET/ITQB and Sciex (Oeiras, Portugal)
2018/11/29	Metabolic profiling of human neural cells for disclosing metabolic determinants and cell-cell interactions	World Preclinical Congress 2018 WPC Europe (Lisbon, Portugal)
2018/06/05	iPSC-derived neurospheroids recapitulate development and pathological signatures of brain microenvironment	3D Cell Culture 2018 DECHEMA (Freiburg, Alemanha)
2017/10/18	Dynamic remodelling of neural cellular and extracellular signatures depicted in 3D in vitro differentiation of human iPSC-derived NSC	25th Anniversary Congress of the European Society for Gene and Cell Therapy ESGCT (Berlin, Alemanha)
2017/05/14	Dynamic remodeling of neural cellular and extracellular signatures depicted in 3D in vitro differentiation of human iPSC-derived NSC	25th European Society for Animal Cell Technology meeting ESACT (Lausanne, Suiça)
2016/10/18	Towards improved predictability in pre-clinical research: Human 3D neural in vitro model for assessment of gene therapy vectors	19th International Congress ESTIV - European Society of Toxicology In Vitro ESTIV (Juan-les-Pins, França)
2015/06/01	Metabolic profiling of human neural cells: combining 3D in vitro models and 13C-NMR Spectroscopy	24th European Society for Animal Cell Technology meeting ESACT (Barcelona, Espanha)
2015/03/11	Towards improved predictability in pre-clinical research: Human 3D neural in vitro model for assessment of gene therapy vectors	5th Spring Meeting ISBiotech (Washington D.C., Estados Unidos)
2012/09/20	Novel Strategies for 3D neural culture & gene delivery: towards human CNS in vitro models for preclinical research	Bioimaging 2012 - 1st International Symposium in Applied Bioimaging Instituto de Engenharia Biomédica (INEB) (Porto, Portugal)

Orientação

	Título / Tema Papel desempenhado	Curso (Tipo) Instituição / Organização
2017/01/01 - Atual	Phenotypic screening for identification of disease-relevant antigens using phage-display technology Coorientador	Biociências Moleculares (Doutoramento) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Júri de grau académico

	Tema Tipo de participação	Nome do candidato (Tipo de grau) Instituição / Organização
2021/03/05	Live cell biosensors for detection and quantification of viral pathogens Arguente principal	Ana Raquel Fernandes (Mestrado) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Curso / Disciplina lecionado

	Disciplina	Curso (Tipo)	Instituição / Organização
2017 - 2020	Modelos in vitro em biomedicina	Medicina (Mestrado integrado)	Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal

Distinções

Prémio

2016	Best Young Scientist Oral Presentation European Society of Toxicology In Vitro (ESTIV), Países Baixos
2013	Registration Award for top 10 selected abstracts for 8th SPCE-TC International meeting Sociedade Portuguesa de Células Estaminais e Terapia Celular (SPCE-TC), Portugal
2010	Highest classification in Molecular Genetics and Biomedicine MSc Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Outra distinção

2016	Travel grant for attendance in the EMBO\|EMBL Symposium: Organoids – Modelling Organ Development and Disease in 3D Culture European Molecular Biology Laboratory, Alemanha
2015	Travel grant for attendance in the BIOMS\|EMBL conference on Physics of Cells and Tissues 2015 - Modeling meets Experiment European Society for Animal Cell Technology (ESACT), Alemanha