I did my PhD on the ¿Effect of oxidized-LDL on endothelial-cell apoptosis and the protective effects of phenolic compounds¿. The work was done in the lab of Prof. R. Salvayre, Univ. Paul Sabatier, Toulouse and my co-supervisor was Prof. L. Almeida, at the Univ. of Coimbra. During my 1st post-doc, I worked on ¿Host-pathogen interactions¿ in the lab of Prof. S. Grinstein, at the Univ. of Toronto. My second post-doc was on ¿The TGN sorting of newly synthesized cargo in polarized cells¿ in the lab of Prof. K. Simons, at the MPI-CBG, Dresden. In 2006, I returned to Portugal to establish myself as an independent scientist at the Center for Neurosciences and Cell Biology at the Univ. of Coimbra. My group worked mainly on the mechanistic understanding of the process of plasma membrane resealing by lysosomes and its regulation during M. tuberculosis infection and on the use of surfactants as topical microbicides to prevent sexually transmitted infections. In 2014, I was awarded with the prestigious iFCT award at the consolidator level and moved to NOVA MEDICAL SCHOOL (NMS), New University of Lisbon, where I am the Principal Investigator of the group -Lysosomes and Disease (https://www.nms.unl.pt/en-us/Research/Research-Groups/Details/investigationgroupid/2219). In NMS taking advantage of my previous experience in atherosclerosis and membrane traffic I started a translational research project on cardiovascular diseases. The main objectives of my research are to identify the biochemical and cell biological etiology of atherosclerosis at the bench and to carry this knowledge through modern analytical chemical techniques to a clinical prediction of CVD risk. The team involved in my research includes cardiologists, molecular cell biologists and biophysical chemists. My group is composed of 1 senior scientist (that is in the World's Top 2% of the highly cited researchers worldwide), 1 assistant researcher, 2 PhD students and 2 research fellows. I have published a total of 50 articles in peer-reviewed journals, 12 as first author, 23 as last author and 25 as corresponding author in high impact journals such as PNAS, Nat. Cell Biol, J. Cell Biol and EBioMedicine. Four of my publications have been highlighted in the J Cell Biol, one in PNAS, one in EBioMedicine and three have been recommended in F1000Prime. Our J. Cell Biol. (2016) article already has 99 citations and was highlighted in J. Cell Biol. and recommended twice in F1000. From the articles published during the last year, 2 were selected for covers of the journals (J. Lipid Res. and Traffic). Currently, I have a manuscript, under revision, in the journal Matter in which I am the last and corresponding author. Excluding the ¿Guidelines for the use and interpretation of assays for monitoring autophagy¿ citations, my publications have been cited more than 5800 times and my h-factor is 33 (Google Scholar). According to Scopus my publications have been cited 5154 times and my h-factor is 30. Since 2006, I completed the supervision of 9 post-doctoral fellows, 7 PhD students and 3 MSc students. In competitive calls for funding, which included the creation of an international consortium with Harvard Medical School, USA, of which I was the coordinator, I have raised around TWO Million euros. I was also awarded with 2 INOVC awards. In addition to my core scientific activities, I am also an Assistant Prof. with habilitation (Tenure Track) in NOVA MEDICAL SCHOOL where I teach more than 200 h/year. I am an Editor for Scientific Reports (Nature Publishing Group) and an Associate Editor for the Frontiers in Cell and Developmental Biology. I also act as examiner in MSc/PhD theses; and participate in peer- and grant proposal reviewing. I was member of the following H2020 consortiums: Twinning, RISE and COST Action. Presently, I am member of the Twinning on Extracellular Vesicles. I have also been involved in science dissemination events. I interrupted my career once on maternity leave in 2007.