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Ana Margarida Fernandes Pereira de Matos graduated in health sciences in 2010, at the University of Lisbon. In the same year she was admitted at the Faculty of Medicine for her masters degree in emerging infectious diseases. At the same time, Ana volunteered as an undergraduate student in two research projects, where she acquired basic training in molecular biology techniques and had her first published scientific contribution (Gama et al. PLoS One. 2013). She graduated from her masters in 2013 with the thesis Characterization of differentially expressed microRNAs in HIV-1 and HIV- 2 infection in Human CD4-T cells: a role for miR-34c-5p in T-cell activation and response to HIV infection, where her work greatly contributed to the publication Amaral et al. EMBO J. 2017. In 2014, Ana obtained a PhD scholarship from FCT under the PhD Programme BioSYS (Biological Systems, Functional & Integrative Genomics), integrated in the BioISI (Biosystems & Integrative Sciences Institute), which she finished successfully in 2018. Her PhD specialty was systems biology, and aimed at finding new modulators of Phe508del-CFTR retention at the plasma membrane (PM) of epithelial cells, and, as a result of her investigation and collaboration with other team members, she authored 4 original papers (Loureiro et al. Sci. Signal. 2015; Matos et al. Sci. Rep. 2018; Loureiro et al. Front. Pharmacol. 2019; Matos et al. JBC. 2019) and a review (Matos, A. M. & Matos J Lung Heal. 2018). At the moment, she is an auxiliary researcher in the project PTDC/BIA-CEL/28408/2017, hired by FCiencias.ID. From May to September 2020, she was invited to integrate the opening team at CTC@CIÊNCIAS, Centro de Testes COVID-19 em Ciências, where she lead and trained the biosafety level 3 cabinet team, and participated in the diagnostic testing of SARS-CoV-2 by RT-qPCR and in the development of new SARS-CoV-2 testing methods for rapid diagnosis. Ana has technical expertise in cell culture, in molecular biology assays and biochemical assays, confocal microscopy, live cell imaging, and BSL3 practice. She also has experience in writing manuscripts, as well as training MSc students. By now she has nearly 100 citations with an index-h of 5, an outcome/a result of her contribution as a team member in five research projects with national and international funding. Moreover, she has presented her work as oral or poster communications, participating in several national and international scientific meetings
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Identificação

Identificação pessoal

Nome completo
Ana Margarida Fernandes Pereira de Matos

Nomes de citação

  • Matos, Ana Margarida
  • Matos, AM
  • Matos, A. M.
  • Matos, Ana M.
  • Matos, Ana M
  • AMM

Identificadores de autor

Ciência ID
6711-5889-A99B
ORCID iD
0000-0002-0160-256X

Endereços de correio eletrónico

  • ana-matos@campus.ul.pt (Profissional)
  • ana.matos@insa.min-saude.pt (Profissional)

Moradas

  • Instituto Nacional de Saúde (INSA) - Doutor Ricardo Jorge. Avenida Padre Cruz, 1649-016 L, Lisboa, Lisboa, Portugal (Profissional)

Websites

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas - Biologia Molecular
  • Ciências Médicas e da Saúde - Ciências da Saúde - Doenças Infeciosas
  • Ciências Médicas e da Saúde - Medicina Básica - Farmacologia e Farmácia
  • Ciências Médicas e da Saúde - Biotecnologia Médica - Tecnologias que envolvem a Manipulação de Células,Tecidos,Órgãos ou todo o Organismo
  • Ciências Naturais - Ciências Biológicas - Biologia Celular
  • Ciências Naturais - Ciências Biológicas - Bioquímica
  • Ciências Médicas e da Saúde - Biotecnologia Médica - Diagnóstico e Terapias de Base Genética

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1)
Inglês Utilizador independente (B1) Utilizador proficiente (C1) Utilizador independente (B1) Utilizador independente (B1)
Formação
Grau Classificação
2014/01/15 - 2018/09/17
Concluído
 Doutoramento em Biologia, Especialidade em Biologia de Sistemas (Doutoramento)
Especialização em Biologia de Sistemas
Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal
"Search for new modulators of Phe508del-CFTR retention at the plasma membrane of epithelial cells " (TESE/DISSERTAÇÃO)
 Aprovada com Distinção
2013/02/28 - 2013/03/01
Concluído
 Biosafety training for use of the BSL3 Facility (Outros)
Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
2013
Concluído
 Curso de Mestrado em Doenças Infecciosas Emergentes (Mestrado)
Universidade de Lisboa Faculdade de Medicina, Portugal
"Characterization of differentially expressed microRNAs in HIV-1 and HIV-2 infection in Human CD4-T cells - A role for miR-34c-5p in T cell activation and response to HIV infection" (TESE/DISSERTAÇÃO)
 Excelente, 19 (Dezanove) valores
2011/11/21 - 2011/11/25
Concluído
 Workshop on Bioinformatics and Systems modelling (Outros)
Universidade de Lisboa Faculdade de Ciências, Portugal
2010
Concluído
 Licenciatura em Ciências da Saúde (Licenciatura)
Universidade de Lisboa, Portugal
"n/a" (TESE/DISSERTAÇÃO)
 Bom, 14 (Catorze) valores
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento 		Empregador
2019/04/01 - 2021/09/30 Investigador Auxiliar (carreira) (Investigação) Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal

Outros

Categoria Profissional
Instituição de acolhimento 		Empregador
2020/04 - 2020/09 Voluntária no CTC@CIÊNCIAS – Centro de Testes COVID-19 em Ciências Universidade de Lisboa Faculdade de Ciências, Portugal
2014 - 2018 Estudante de Doutoramento (PhD Student) com o projeto "Search for new modulators of Phe508del-CFTR retention at the plasma membrane of epithelial cells" Universidade de Lisboa Faculdade de Ciências, Portugal
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
(...)
2011 - 2013 Estudante de Mestrado com o projeto "Characterization of differentially expressed microRNAs in HIV-1 and HIV- 2 infection in Human CD4-T cells" Universidade de Lisboa Faculdade de Ciências, Portugal
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
(...)
2010/10 - 2011/09 Voluntária no projeto de Investigação "Validation of SMN interacting RBPs identified by Mass-spectrometry." Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
Projetos

Bolsa

Designação Financiadores
2015 - 2016 Pesquisa de novos alvos moleculares para adjuvar a correção farmacológica da F508delCFTR
PGG/055/2014
Membro da equipa
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal

Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
Gilead Sciences
Concluído

Projeto

Designação Financiadores
2019/04/01 - Atual New signaling pathways involved in the regulation of chloride sodium transport proteins
PTDC/BIA-CEL/28408/2017
Investigador
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
 Fundação para a Ciência e a Tecnologia
Em curso
2016 - 2018 Protein networks stabilizing CFTR at the plasma membrane, an integrated interactomics approach to find novel therapeutic targets in CF
BioISI/96202-P0.b/2015
Membro da equipa
Universidade de Lisboa Instituto de Biossistemas e Ciências Integrativas, Portugal
2011 - 2013 Modulation of HIV Replication and Latency in Human CD4 T Cells by Regulatory Small RNAs
Merck LKR 101361
Membro da equipa
Concluído
2010 - 2011 Characterization of post-transcriptional regulatory mechanisms of SMN2
SMA-EUROPE 2008 / PROJECT N° 13185
Membro da equipa
Produções

Publicações

Artigo em jornal
  1. Matos, Ana; Matos, Paulo. "Combination therapy in Phe508del CFTR: how many will be enough?", Lung Health Dis. 2, 9–16, 2018, http://www.lungdiseasesjournal.com/articles/combination-therapy-in-phe508del-cftr-how-many-will-be-enough.html.
Artigo em revista
  1. Matos, Ana Margarida; Jordan, Peter; Matos, Paulo. "Treatment of Polarized Cystic Fibrosis Airway Cells With HGF Prevents VX-661-Rescued F508del-CFTR Destabilization Caused by Prolonged Co-exposure to VX-770". Frontiers in Molecular Biosciences 8 (2021): http://dx.doi.org/10.3389/fmolb.2021.812101.
    10.3389/fmolb.2021.812101
  2. Loureiro, Cláudia Almeida; Santos, João D.; Matos, Ana Margarida; Jordan, Peter; Matos, Paulo; Farinha, Carlos M.; Pinto, Francisco R.. "Network Biology Identifies Novel Regulators of CFTR Trafficking and Membrane Stability". Frontiers in Pharmacology 10 (2019): https://doi.org/10.3389/fphar.2019.00619.
    10.3389/fphar.2019.00619
  3. Matos, A.M.; Pinto, F.R.; Barros, P.; Amaral, M.D.; Pepperkok, R.; Matos, P.. "Inhibition of calpain 1 restores plasma membrane stability to pharmacologically rescued Phe508del-CFTR variant". Journal of Biological Chemistry 294 36 (2019): 13396-13410. http://www.scopus.com/inward/record.url?eid=2-s2.0-85071831853&partnerID=MN8TOARS.
    10.1074/jbc.RA119.008738
  4. Ana M. Matos; Andreia Gomes-Duarte; Márcia Faria; Patrícia Barros; Peter Jordan; Margarida D. Amaral; Paulo Matos. "Prolonged co-treatment with HGF sustains epithelial integrity and improves pharmacological rescue of Phe508del-CFTR". Scientific Reports (2018): https://doi.org/10.1038/s41598-018-31514-2.
    10.1038/s41598-018-31514-2
  5. Andreia J Amaral; Jorge Andrade; Russell B Foxall; Paula Matoso; Ana M Matos; Rui S Soares; Cheila Rocha; et al. "miRNA profiling of human naive CD4 T cells links miR-34c-5p to cell activation and HIV replication". The EMBO Journal 36 3 (2017): 346-360. https://doi.org/10.15252/embj.201694335.
    10.15252/embj.201694335
  6. Loureiro CA; Matos AM; Dias-Alves Â; Pereira JF; Uliyakina I; Barros P; Amaral MD; Matos P. "A molecular switch in the scaffold NHERF1 enables misfolded CFTR to evade the peripheral quality control checkpoint.". Science Signaling (2015): https://stke.sciencemag.org/content/8/377/ra48.full.
    10.1126/scisignal.aaa1580
  7. Gama JA; Reis AM; Domingues I; Mendes-Soares H; Matos AM; Dionisio F. "Temperate bacterial viruses as double-edged swords in bacterial warfare.". Plos One (2013): https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0059043.
    10.1371/journal.pone.0059043
Poster em conferência
  1. Matos, Ana Margarida; Pinto, Francisco; Barros, Patrícia; Amaral, Margarida; Pepperkok, Rainer; Matos, Paulo. "Plasma membrane-specific interactome analysis reveals calpain 1 as a druggable modulator of rescued Phe508del-CFTR cell surface stability". Trabalho apresentado em SINAL 2019 – 10th Meeting on Signal Transduction, 2019.
    10.1109/elektro.2016.7512136
  2. Matos, Ana Margarida; Barros, Patrícia; Pepperkok, Rainer; Matos, Paulo. "Search for new modulators of rescued F508del-CFTR retention at the plasma membrane". Trabalho apresentado em BioSys-BioISI 2nd Summer Retreat, 2017.
  3. Neno, Vanessa; Matos, Ana Margarida; Matos, Paulo. "Characterization of Ezrin-mediated stabilization of rescued F508del-CFTR at the surface of airway cells". Trabalho apresentado em Dia do Jovem Investigador do Instituto Ricardo Jorge 2017, 2017.
  4. Matos, Ana Margarida; Matos, Paulo. "Search for new modulators of F508del-CFTR retention at the plasma membrane of epithelial cells". Trabalho apresentado em Dia do Jovem Investigador do Instituto Ricardo Jorge 2017, 2017.
  5. Loureiro, Claudia A.; Matos, Ana Margarida; Dias-Alves, Ângela; Pereira, Joana F; Uliyakina, Inna; Barros, Patrícia; Amaral, Margarida; Matos, Paulo. "A NHERF1 PDZ domain switch averts CHIP Ub ligase-mediated peripheral membrane quality control of misfolded CFTR". Trabalho apresentado em European Cystic Fibrosis Society, 14th ECFS Basic Science Conference, 2017.
  6. Neno, Vanessa; Matos, Ana Margarida; Matos, Paulo. "Design of cell-penetrating peptides to stabilize rescued F508del-CFTR at the surface of epithelial cells". Trabalho apresentado em SINAL 2017 - 8th Meeting on Signal Transduction, 2017.
  7. Matos, Ana Margarida; Barros, Patrícia; Matos, Paulo. "A new optimization approach for the automatic design of ΣΔ-modulators". Trabalho apresentado em SINAL 2017 - 8th Meeting on Signal Transduction, 2017.
  8. Matos, Ana Margarida; Pepperkok, Rainer; Matos, Paulo. "siRNA screen for modulators of CFTR surface retention". Trabalho apresentado em Advanced Lecture Course on Systems Biology, SYSBIO 2016, 2016.
  9. Matos, Ana Margarida; Pereira, Joana F; Uliyakina, Inna; Amaral, Margarida; Matos, Paulo. "Anchoring to the actin-cytoskeleton is not essential to retain functional F508del-CFTR at the plasma membrane". Trabalho apresentado em European Cystic Fibrosis Society, 12th ECFS Basic Science Conference, 2015.
  10. Gama, João Alves; Domingues, Iolanda; Reis, Ana Maria; Mendes-Soares, Helena; Matos, Ana Margarida; Carvalho, André; Dionisio, Francisco. "Mutualistic parasites". Trabalho apresentado em Congress of the European Society for Evolutionary Biology, 2013.
  11. Matos, Ana Margarida; Foxall, Russell B; Amaral, Andreia J.; Andrade, Jorge; Matoso, Paula; Santa-Marta, Mariana; Tendeiro, Rita; et al. "miR-34c-5p at the crossroads of human naive CD4 T-cell activation and HIV infection". Trabalho apresentado em Molecular Biology in Portugal and EMBL (and EMBL alumni meeting), 2013.
  12. Fernandes, Ana Miguel; Gomes, Ana Luísa; Oliveira, Mariana; Costa, Gonçalo; Peixeiro, Isabel; Matos, Ana Margarida; Cordeiro, Carlos; Gama-Carvalho, Margarida. "Post-transcriptional regulation of SMN2 expression by hnRNP G and LARP Family proteins". Trabalho apresentado em RNA 2013 Davos, 2013.
  13. Matos, Ana Margarida; Foxall, Russell B; Amaral, Andreia J.; Andrade, Jorge; Matoso, Paula; Santa-Marta, Mariana; Tendeiro, Rita; et al. "miR-34c-5p at the crossroads of human naive CD4 T-cell activation and HIV infection". Trabalho apresentado em XXXVIII Annual Meeting of the Portuguese Society of Immunology, From immune disorders to immunotherapies, 2012.
  14. Gomes, Ana Luísa; de Oliveira, Hermenegildo Borges; Matos, Ana Margarida; Margarida Gama-Carvalho. "Regulation of SMN mRNA expression by LARP4 and hnRNPG RNA binding proteins - potential targets for Spinal Muscular Atrophy". Trabalho apresentado em FEBS International Workshop, "New Developments in RNA Biology", 2012.
  15. Andrade, Jorge; Amaral, Andreia J.; Matos, Ana Margarida; Santa-Marta, M; Matoso, Paula; Soares, Rui; Foxall, Russell B; et al. "Novel species of tRNA derived small-RNAs are specifically induced in T cells in response to HIV infection". Trabalho apresentado em FEBS International Workshop, "New Developments in RNA Biology", 2012.
  16. Amaral, Andreia J.; Andrade, Jorge; Matos, Ana Margarida; Matoso, Paula; Soares, Rui; Foxall, Russell B; Tendeiro, Rita; et al. "Deep-sequencing profile of small ncRNAs in human CD4+ T cells upon TCR-mediated activation and its modulation by HIV infection". Trabalho apresentado em FEBS International Workshop, "New Developments in RNA Biology", 2012.
  17. Matos, Ana Margarida; Andrade, Jorge; Amaral, Andreia J.; Santa-Marta, M; Serra-Caetano, Ana; Foxall, Russell B; Matoso, Paula; et al. "A role for miR-34c-5p in T-cell activation and response to HIV infection". Trabalho apresentado em FEBS International Workshop, "New Developments in RNA Biology", 2012.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2017 Signaling the functional rescue of mutant CFTR in Cystic Fibrosis SINAL 2017 - 8th Meeting on Signal Transduction
(Lisboa, Portugal)
2013/06/11 miR-34c-5p is a novel regulator of naive T-cell activation that impacts HIV replication RNA 2013
(Davos, Suiça)
2012/12/21 Mutualistic Parasites: when hosts and parasites have their interests aligned VIII Encontro Nacional de Biologia Evolutiva
(Oeiras, Portugal)
2012/12/06 Bacteria Use Viruses as Biological Weapons TiBE2012, Trends in Biodiversity and Evolution: Integrative Approaches in Evolutionary Biology
(Porto, Portugal)
2012/09/01 Small non-coding RNAs: central players in the CD4+ T cell response to retroviral infection FEBS International Workshop, "New Developments in RNA Biology"
FEBS (Tavira, Portugal)
Distinções

Prémio

2016 SysBio2016 Course
2015 Student Helper Award at the 12th Basic Science Conference
European Cystic Fibrosis Society, Dinamarca

Outra distinção

2014 phD Fellowship - Biosys PhD program