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André R.A. Marques graduated in Biochemistry (BSc, University of Lisbon; MSc, University of Amsterdam) and currently works as Assistant Researcher at the NOVA Medical School (NMS, Portugal). He obtained his Ph.D. in Medical Biochemistry at the University of Amsterdam, The Netherlands. During his Ph.D. studies he investigated the role of lysosomal homeostasis in neurodegeneration. Afterwards, André developed a 3-year PostDoctoral Project at the University of Kiel (Germany), centred on Batten disease (NCL2TREAT Consortium), the most common inherited neurodegenerative disease in children. This project resulted in the registration of an international patent for the treatment of Batten disease, of which he is co-inventor. Since 2019, A. Marques has been developing a project investigating the role of lysosomal dysfunction in atherosclerosis at the NMS. Last year, he was awarded a position as Assistant Researcher by the Portuguese Foundation for Science and Technology (FCT), to develop a 6-year project focused on the interplay between lipid metabolism and organelle dysfunction in human disease. During the course of his career, A. R. A. Marques has published 31 scientific articles (h index = 18, Citations = 1181, Source = Scopus) and was awarded two research grants from the FCT. He has also participated in numerous international scientific conferences and workshops, having been selected to present his work on four occasions, including at a Gordon Research Conference. Furthermore, he has supervised several BSc, MSc and PhD students, and a M.D. doctoral thesis.
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Identificação

Identificação pessoal

Nome completo
André Rosa Alcalde Marques

Nomes de citação

  • Marques, André R. A.

Identificadores de autor

Ciência ID
7512-8D9D-138D
ORCID iD
0000-0001-9674-3017

Endereços de correio eletrónico

  • andre.marques@nms.unl.pt (Profissional)

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas - Bioquímica
Formação
Grau Classificação
2011/09/01 - 2016/06/08
Concluído
 Medical Biochemistry (Doctor of Philosophy)
Academisch Medisch Centrum Faculteit Geneeskunde, Países Baixos

Universiteit van Amsterdam, Países Baixos
"Lysosomal glycosidases and glycosphingolipids: new avenues for research" (TESE/DISSERTAÇÃO)
2009/09/01 - 2011/07/29
Concluído
 Medical Biochemistry (Master)
Universiteit van Amsterdam Faculteit der Natuurwetenschappen Wiskunde en Informatica, Países Baixos
"Detection of glucocerebrosidase with fluorescent suicide inhibitors " (TESE/DISSERTAÇÃO)
 8.5/10 Cum laude
2006/09/01 - 2009/07/15
Concluído
 Bioquímica (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
 17/20
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento 		Empregador
2025/02/01 - Atual Investigador Auxiliar (carreira) (Investigação) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
Universidade Nova de Lisboa Medical School, Portugal
2019/03/01 - 2025/01/31 Investigador Contratado (Investigação) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2016/03/01 - 2018/01/31 Pós-doutorado (Investigação) Christian-Albrechts-Universität zu Kiel Biochemisches Institut, Alemanha
Christian-Albrechts-Universität zu Kiel Biochemisches Institut, Alemanha
2011/09/01 - 2016/02/29 Investigador (Investigação) Academisch Medisch Centrum Faculteit Geneeskunde, Países Baixos
Academisch Medisch Centrum Faculteit Geneeskunde, Países Baixos
2010/09/01 - 2011/05/31 Estagiário de Investigação (Investigação) Academisch Medisch Centrum Faculteit Geneeskunde, Países Baixos
Academisch Medisch Centrum Faculteit Geneeskunde, Países Baixos
2009/09/01 - 2010/06/30 Estagiário de Investigação (Investigação) Universiteit van Amsterdam Swammerdam Institute for Life Sciences, Países Baixos
Universiteit van Amsterdam Swammerdam Institute for Life Sciences, Países Baixos
2008/12/01 - 2009/08/31 Estagiário de Investigação (Investigação) Universidade de Lisboa Departamento de Química e Bioquímica, Portugal
Universidade de Lisboa Departamento de Química e Bioquímica, Portugal
Produções

Publicações

Artigo em revista
  1. Elizeth Lopes; Gisela Machado-Oliveira; Catarina Guerreiro Simões; Inês S. Ferreira; Cristiano Ramos; José Ramalho; Maria I. L. Soares; et al. "Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts". Cells (2023): https://doi.org/10.3390/cells12242826.
    10.3390/cells12242826
  2. Neuza Domingues; Joana Gaifem; Rune Matthiesen; Diana P. Saraiva; Luís Bento; André R.A. Marques; Maria I.L. Soares; et al. "Cholesteryl hemiazelate identified in CVD patients causes in vitro and in vivo inflammation". Journal of Lipid Research (2023): https://doi.org/10.1016/j.jlr.2023.100419.
    10.1016/j.jlr.2023.100419
  3. Neuza Domingues; Marques, André R. A.; Rita Diogo Almeida Calado; Inês S. Ferreira; Cristiano Ramos; José Ramalho; Maria I. L. Soares; et al. Autor correspondente: Otília V. Vieira. "Oxidized cholesteryl ester induces exocytosis of dysfunctional lysosomes in lipidotic macrophages". Traffic (2023): https://doi.org/10.1111/tra.12888.
    Publicado • 10.1111/tra.12888
  4. Yen-Hsi Chen; Weihua Tian; Makiko Yasuda; Zilu Ye; Ming Song; Ulla Mandel; Claus Kristensen; et al. "A universal GlycoDesign for lysosomal replacement enzymes to improve circulation time and biodistribution". Frontiers in Bioengineering and Biotechnology 11 (2023): http://dx.doi.org/10.3389/fbioe.2023.1128371.
    Publicado • 10.3389/fbioe.2023.1128371
  5. Chi-Lin Kuo; Qin Su; Adrianus M. C. H. van den Nieuwendijk; Thomas J.M. Beenakker; Wendy A. Offen; Lianne Irene Willems; Rolf. G. Boot; et al. "The development of a broad-spectrum retaining ß-exo-galactosidase activity-based probe". Organic & Biomolecular Chemistry (2023): http://dx.doi.org/10.1039/d3ob01261a.
    10.1039/d3ob01261a
  6. Lisa Gallwitz; Lina Schmidt; André R. A. Marques; Andreas Tholey; Liam Cassidy; Irem Ulku; Gerhard Multhaup; Alessandro Di Spiezio; Paul Saftig. "Cathepsin D: Analysis of its potential role as an amyloid beta degrading protease". Neurobiology of Disease (2022): 105919-105919. http://dx.doi.org/10.1016/j.nbd.2022.105919.
    Publicado • 10.1016/j.nbd.2022.105919
  7. Eva Alegre-Cortés; Alberto Giménez-Bejarano; Elisabet Uribe-Carretero; Marta Paredes-Barquero; André R. A. Marques; Mafalda Lopes-da-Silva; Otília V. Vieira; et al. "Delay of EGF-Stimulated EGFR Degradation in Myotonic Dystrophy Type 1 (DM1)". Cells 11 19 (2022): 3018-3018. http://dx.doi.org/10.3390/cells11193018.
    Publicado • 10.3390/cells11193018
  8. Barral, Duarte C.; Staiano, Leopoldo; Guimas Almeida, Cláudia; Cutler, Dan F.; Eden, Emily R.; Futter, Clare E.; Galione, Antony; et al. "Current methods to analyze lysosome morphology, positioning, motility and function". Traffic 23 5 (2022): 238-269. http://dx.doi.org/10.1111/tra.12839.
    Publicado • 10.1111/tra.12839
  9. Prieto Huarcaya, S.; Drobny, A.; Marques, André R. A.; Di Spiezio, Alessandro; Dobert, J. P.; Balta, D.; Werner, C.; et al. Autor correspondente: Zunke, Friederike. "Recombinant pro-CTSD (cathepsin D) enhances SNCA/alpha-Synuclein degradation in alpha-Synucleinopathy models". Autophagy (2022):
    Publicado • 10.1080/15548627.2022.2045534
  10. Alves, Liliana S.; Marques, André R. A.; Padrão, Nuno; Carvalho, Filomena A.; Ramalho, José; Lopes, Catarina S.; Soares, Maria I. L.; et al. Autor correspondente: Marques, André R. A.. "Cholesteryl hemiazelate causes lysosome dysfunction impacting vascular smooth muscle cell homeostasis". Journal of Cell Science 135 5 (2021): http://dx.doi.org/10.1242/jcs.254631.
    Publicado • 10.1242/jcs.254631
  11. Di Spiezio, Alessandro; Marques, André R. A.; Schmidt, Lina; Thießen, Niklas; Gallwitz, Lisa; Fogh, Jens; Bartsch, Udo; Saftig, Paul. Autor correspondente: Saftig, Paul. "Analysis of cathepsin B and cathepsin L treatment to clear toxic lysosomal protein aggregates in neuronal ceroid lipofuscinosis". Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1867 10 (2021): 166205. http://dx.doi.org/10.1016/j.bbadis.2021.166205.
    Publicado • 10.1016/j.bbadis.2021.166205
  12. van der Lienden, Martijn J. C.; Aten, Jan; Marques, André R. A.; Waas, Ingeborg S. E.; Larsen, Per W. B.; Claessen, Nike; van der Wel, Nicole N.; et al. Autor correspondente: Aerts, Johannes M. F. G.. "GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice". International Journal of Molecular Sciences 22 5 (2021): 2532. http://dx.doi.org/10.3390/ijms22052532.
    Acesso aberto • Publicado • 10.3390/ijms22052532
  13. Marques, André R. A.; Ramos, Cristiano; Machado-Oliveira, Gisela; Vieira, Otília V.. Autor correspondente: Marques, André R. A.. "Lysosome (Dys)function in Atherosclerosis - A Big Weight on the Shoulders of a Small Organelle". Frontiers in Cell and Developmental Biology 9 (2021): http://dx.doi.org/10.3389/fcell.2021.658995.
    Publicado • 10.3389/fcell.2021.658995
  14. Boer, Daphne E.; Mirzaian, Mina; Ferraz, Maria J.; Zwiers, Kimberley C.; Baks, Merel V.; Hazeu, Marc D.; Ottenhoff, Roelof; et al. Autor correspondente: Aerts, Johannes M.. "Human glucocerebrosidase mediates formation of xylosyl-cholesterol by ß-xylosidase and transxylosidase reactions". Journal of Lipid Research 62 (2021): 100018. http://dx.doi.org/10.1194/jlr.ra120001043.
    Publicado • 10.1194/jlr.ra120001043
  15. Machado-Oliveira, Gisela; Ramos, Cristiano; Marques, André R. A.; Vieira, Otília V.. Autor correspondente: Vieira, Otília V.. "Cell Senescence, Multiple Organelle Dysfunction and Atherosclerosis". Cells 9 10 (2020): 2146. http://dx.doi.org/10.3390/cells9102146.
    Publicado • 10.3390/cells9102146
  16. Herrera Moro Chao, Daniela; Wang, Yanan; Foppen, Ewout; Ottenhoff, Roelof; van Roomen, Cindy; Parlevliet, Edwin T.; van Eijk, Marco; et al. Autor correspondente: Yi, Chun-Xia. "The Iminosugar AMP-DNM Improves Satiety and Activates Brown Adipose Tissue Through GLP1". Diabetes 68 12 (2019): 2223-2234. http://dx.doi.org/10.2337/db19-0049.
    Publicado • 10.2337/db19-0049
  17. Marques, André R. A.; Di Spiezio, Alessandro; Thießen, Niklas; Schmidt, Lina; Grötzinger, Joachim; Lüllmann-Rauch, Renate; Damme, Markus; et al. Autor correspondente: Saftig, Paul. "Enzyme replacement therapy with recombinant pro-CTSD (cathepsin D) corrects defective proteolysis and autophagy in neuronal ceroid lipofuscinosis". Autophagy 16 5 (2019): 811-825. http://dx.doi.org/10.1080/15548627.2019.1637200.
    Publicado • 10.1080/15548627.2019.1637200
  18. Marques, André R. A.; Saftig, Paul. Autor correspondente: Saftig, Paul. "Lysosomal storage disorders - challenges, concepts and avenues for therapy: beyond rare diseases". Journal of Cell Science 132 2 (2019): jcs221739. http://dx.doi.org/10.1242/jcs.221739.
    Publicado • 10.1242/jcs.221739
  19. van Rooden, Eva J.; van Esbroeck, Annelot C. M.; Baggelaar, Marc P.; Deng, Hui; Florea, Bogdan I.; Marques, André R. A.; Ottenhoff, Roelof; et al. Autor correspondente: van der Stelt, Mario. "Chemical Proteomic Analysis of Serine Hydrolase Activity in Niemann-Pick Type C Mouse Brain". Frontiers in Neuroscience 12 (2018): http://dx.doi.org/10.3389/fnins.2018.00440.
    Publicado • 10.3389/fnins.2018.00440
  20. Aerts, Johannes M. F. G.; Ferraz, Maria J; Mirzaian, Mina; Gaspar, Paulo; Oussoren, Saskia V; Wisse, Patrick; Chi-Lin Kuo; et al. "Lysosomal Storage Diseases. For Better or Worse: Adapting to Defective Lysosomal Glycosphingolipid Breakdown". eLS (2017): http://onlinelibrary.wiley.com/doi/10.1002/9780470015902.a0027592/abstract.
    Publicado • 10.1002/9780470015902.a0027592
  21. Mirzaian, M.; Wisse, P.; Ferraz, M.J.; Marques, André R. A.; Gaspar, P.; Oussoren, S.V.; Kytidou, K.; et al. Autor correspondente: Aerts, J.M.. "Simultaneous quantitation of sphingoid bases by UPLC-ESI-MS/MS with identical 13C-encoded internal standards". Clinica Chimica Acta 466 (2017): 178-184. http://dx.doi.org/10.1016/j.cca.2017.01.014.
    Publicado • 10.1016/j.cca.2017.01.014
  22. Marques, André R. A.; Willems, Lianne I.; Herrera Moro, Daniela; Florea, Bogdan I.; Scheij, Saskia; Ottenhoff, Roelof; van Roomen, Cindy P. A. A.; et al. Autor correspondente: Aerts, Johannes M. F. G.. "A Specific Activity-Based Probe to Monitor Family GH59 Galactosylceramidase, the Enzyme Deficient in Krabbe Disease". ChemBioChem 18 4 (2017): 402-412. http://dx.doi.org/10.1002/cbic.201600561.
    Publicado • 10.1002/cbic.201600561
  23. Mirzaian, Mina; Wisse, Patrick; Ferraz, Maria J.; Marques, André R. A.; Gabriel, Tanit L.; van Roomen, Cindy P.A.A.; Ottenhoff, Roelof; et al. Autor correspondente: Aerts, Johannes M.. "Accurate quantification of sphingosine-1-phosphate in normal and Fabry disease plasma, cells and tissues by LC-MS/MS with 13 C-encoded natural S1P as internal standard". Clinica Chimica Acta 459 (2016): 36-44. http://dx.doi.org/10.1016/j.cca.2016.05.017.
    Publicado • 10.1016/j.cca.2016.05.017
  24. Ferraz, Maria J.; Marques, André R. A.; Appelman, Monique D.; Verhoek, Marri; Strijland, Anneke; Mirzaian, Mina; Scheij, Saskia; et al. Autor correspondente: Aerts, Johannes M.. "Lysosomal glycosphingolipid catabolism by acid ceramidase: formation of glycosphingoid bases during deficiency of glycosidases". FEBS Letters 590 6 (2016): 716-725. http://dx.doi.org/10.1002/1873-3468.12104.
    Publicado • 10.1002/1873-3468.12104
  25. Marques, André R. A.; Mirzaian, Mina; Akiyama, Hisako; Wisse, Patrick; Ferraz, Maria J.; Gaspar, Paulo; Ghauharali-van der Vlugt, Karen; et al. Autor correspondente: Aerts, Johannes M.. "Glucosylated cholesterol in mammalian cells and tissues: formation and degradation by multiple cellular ß-glucosidases". Journal of Lipid Research 57 3 (2016): 451-463. http://dx.doi.org/10.1194/jlr.m064923.
    Publicado • 10.1194/jlr.m064923
  26. Ferraz, Maria J.; Marques, André R. A.; Gaspar, Paulo; Mirzaian, Mina; van Roomen, Cindy; Ottenhoff, Roelof; Alfonso, Pilar; et al. Autor correspondente: Aerts, Johannes M.. "Lyso-glycosphingolipid abnormalities in different murine models of lysosomal storage disorders". Molecular Genetics and Metabolism 117 2 (2016): 186-193. http://dx.doi.org/10.1016/j.ymgme.2015.12.006.
    Publicado • 10.1016/j.ymgme.2015.12.006
  27. Marques, André R. A.; Gabriel, Tanit L.; Aten, Jan; van Roomen, Cindy P. A. A.; Ottenhoff, Roelof; Claessen, Nike; Alfonso, Pilar; et al. Autor correspondente: van Eijk, Marco. "Gpnmb Is a Potential Marker for the Visceral Pathology in Niemann-Pick Type C Disease". PLOS ONE 11 1 (2016): e0147208. http://dx.doi.org/10.1371/journal.pone.0147208.
    Publicado • 10.1371/journal.pone.0147208
  28. Herrera Moro Chao, Daniela; Kallemeijn, Wouter W.; Marques, André R. A.; Orre, Marie; Ottenhoff, Roelof; van Roomen, Cindy; Foppen, Ewout; et al. Autor correspondente: Aerts, Johannes M. F. G.. "Visualization of Active Glucocerebrosidase in Rodent Brain with High Spatial Resolution following In Situ Labeling with Fluorescent Activity Based Probes". PLOS ONE 10 9 (2015): e0138107. http://dx.doi.org/10.1371/journal.pone.0138107.
    Publicado • 10.1371/journal.pone.0138107
  29. Marques, André R. A.; Aten, Jan; Ottenhoff, Roelof; van Roomen, Cindy P. A. A.; Herrera Moro, Daniela; Claessen, Nike; Vinueza Veloz, María Fernanda; et al. Autor correspondente: Aerts, Johannes M. F. G.. "Reducing GBA2 Activity Ameliorates Neuropathology in Niemann-Pick Type C Mice". PLOS ONE 10 8 (2015): e0135889. http://dx.doi.org/10.1371/journal.pone.0135889.
    Publicado • 10.1371/journal.pone.0135889
  30. Dahl, Maria; Doyle, Alexander; Olsson, Karin; Månsson, Jan-Eric; Marques, André R. A.; Mirzaian, Mina; Aerts, Johannes M; et al. Autor correspondente: Karlsson, Stefan. "Lentiviral Gene Therapy Using Cellular Promoters Cures Type 1 Gaucher Disease in Mice". Molecular Therapy 23 5 (2015): 835-844. http://dx.doi.org/10.1038/mt.2015.16.
    Publicado • 10.1038/mt.2015.16
  31. Ferraz, Maria J.; Kallemeijn, Wouter W.; Mirzaian, Mina; Herrera Moro, Daniela; Marques, André R. A.; Wisse, Patrick; Boot, Rolf G.; et al. Autor correspondente: Aerts, J.M.. "Gaucher disease and Fabry disease: New markers and insights in pathophysiology for two distinct glycosphingolipidoses". Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1841 5 (2014): 811-825. http://dx.doi.org/10.1016/j.bbalip.2013.11.004.
    Publicado • 10.1016/j.bbalip.2013.11.004
  32. Sorrentino, Vincenzo; Nelson, Jessica K.; Maspero, Elena; Marques, André R. A.; Scheer, Lilith; Polo, Simona; Zelcer, Noam. Autor correspondente: Zelcer, Noam. "The LXR-IDOL axis defines a clathrin-, caveolae-, and dynamin-independent endocytic route for LDLR internalization and lysosomal degradation". Journal of Lipid Research 54 8 (2013): 2174-2184. http://dx.doi.org/10.1194/jlr.m037713.
    Publicado • 10.1194/jlr.m037713
  33. Kallemeijn, Wouter W.; Li, Kah-Yee; Witte, Martin D.; Marques, André R. A.; Aten, Jan; Scheij, Saskia; Jiang, Jianbing; et al. Autor correspondente: Overkleeft, Herman S.. "Novel Activity-Based Probes for Broad-Spectrum Profiling of Retaining ß-Exoglucosidases In Situ and In Vivo". Angewandte Chemie International Edition 51 50 (2012): 12529-12533. http://dx.doi.org/10.1002/anie.201207771.
    Publicado • 10.1002/anie.201207771
Poster em conferência
  1. Guerreiro Simões, Catarina; Lopes, Elizeth; Ferreira, Inês S.; Ribeiro, Quélia; Ramalho, José; Marques, André R. A.; Vieira, Otília V. Autor correspondente: Vieira, Otília V. "Unravelling the Connection Between Lysosomal Dysfunction and Senescence in Atherogenesis". Trabalho apresentado em NMS PhD Retreat 2024 - Lisbon, 2024.
  2. Inês S. Ferreira; Ribeiro, Quélia; Elizeth Lopes; Guerreiro Simões, Catarina; Hall, Michael J; Ramalho, Jose; Marques, André R. A.; Otilia V. Vieira. "Exploring the Effects of Oxidized Lipids on Murine Macrophages: A Pathway to Atherosclerosis". Trabalho apresentado em International FOR2625 Symposium on LYSOSOMES & AUTOPHAGY, 2024.
  3. Inês S. Ferreira; Ribeiro, Quélia; Elizeth Lopes; Guerreiro Simões, Catarina; Hall, Michael J; Ramalho, Jose; Marques, André R. A.; Vieira, Otília V. "Unravelling Atherosclerosis: Exploring Lysosomal Dysfunction and Cell Death in Murine Macrophages Exposed to Oxidized Lipids". Trabalho apresentado em 2nd MIA-Portugal Ageing Spring School, 2024.
  4. Marques, André R. A.; Ferraz, Maria J.; Ferreira, Inês S.; Lopes, Elizeth; Guerreiro Simões, Catarina; Aerts, Johannes M. F. G.; Vieira, Otília V. "Atherosclerotic plaques accumulate glucosylated cholesterol in response to lysosomal glycosphingolipid and cholesterol storage". Trabalho apresentado em Glycolipid and Sphingolipid Biology Gordon Research Conference, 2024.
  5. Luís Fialho; Inês S. Ferreira; Guerreiro Simões, Catarina; Lopes, Elizeth; Marques, André R. A.; Ramalho, José; Vieira, Otília V. "Cellular and Lysosomal Dysfunction Induced In Vitro by Cholesteryl Hemiglutarate in RAW264.7 Cells". Trabalho apresentado em Programa de Integração de Alunos em Trabalhos de Investigação (PIATI), 2023.
  6. Lopes, Elizeth; Machado-Oliveira, Gisela; Guerreiro Simões, Catarina; Inês S. Ferreira; Ramos, Cristiano; Ramalho, José; Maria I. L. Soares; et al. "The pro-atherogenic lipid cholesteryl hemiazelate induces lysosome dysfunction in murine fibroblasts". Trabalho apresentado em 1st NMS Research Week, 2023.
  7. Inês S. Ferreira; Lopes, Elizeth; Guerreiro Simões, Catarina; Marques, André R. A.; Vieira, Otília V.. "The Impact of Oxidized Lipids in the Homeostasis of Murine Macrophages: Insights into Atherosclerosis". Trabalho apresentado em 1st NMS Research Week, 2023.
  8. L. Alves; Marques, André R. A.; N. Padrão; F.A. Carvalho; C. Lopes; Maria I. L. Soares; Teresa M. V. D. Pinho e Melo; N. C. Santos; O. V. Vieira. "Lysosomal dysfunction in vascular smooth muscle cells plays a role in atherosclerosis". Trabalho apresentado em Workshop on Lysosome Biology - 2019 EDITION, 2019.

Propriedade Intelectual

Patente
  1. Marques, André R. A.; Fogh, Jens; Saftig, Paul; Zunke, Friederike. 2019. "Treatment of neuronal ceroid lipofuscinosis". Estados Unidos.
    Concedida/Emitida