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I have a 5-year degree in Plant Biology from the University of Lisbon and a Ph.D. in Virology from the University of Oxford, where I worked with vaccinia virus, a poxvirus, under the supervision of Prof. Geoffrey Smith. Before and after my Ph.D. I worked in the Laboratory of Virology at the Instituto Nacional de Saúde in Lisbon, as a diagnostic virologist, also in charge of quality control and seroepidemiological studies. I joined Prof. Pedro Simas viral pathogenesis lab as a post-doc, first at the Instituto Gulbenkian de Ciência, and later at the Instituto de Medicina Molecular in Lisbon, where I had a research contract until 2024. My research focused in understanding the function of gammaherpesvirus (e.g., Kaposi´s sarcoma-associated herpesvirus) proteins that are key to viral latency. Latency is linked to disease in this family of viruses. I am currently working at the Gulbenkian Institute for Molecular Medicine, Lisbon, in the lab of Prof. Miguel castanho, assessing anti-viral compounds, namely against SARS-CoV-2. I am interested in virus - host interactions. I have a wide range of skills in virus methods, virus genome manipulation, in vitro and in vivo infection and have worked in biosafety level 2 and 3 conditions. I am also skilled in the characterization of protein-protein interactions using techniques such as pull-down assays and surface plasmon resonance.
Identificação

Identificação pessoal

Nome completo
Marta Pires de Miranda

Identificadores de autor

Ciência ID
8A1D-8B1A-205B
ORCID iD
0000-0003-3733-4963
Scopus Author Id
14008468100

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas - Virologia
Formação
Grau Classificação
2016 - 2016
Concluído
Personal licence for carrying out procedures in animals (Function A) granted by Direção Geral de Alimentação e Veterinária, Portuguese Government (Outros)
Direção - Geral de Alimentação e Veterinária , Portugal
1998/01/01 - 2002/10/26
Concluído
Virology (Doctor of Philosophy)
University of Oxford, Reino Unido
"Studies on vaccinia virus C2L: a member of the kelch family of proteins" (TESE/DISSERTAÇÃO)
D.Phil.
1996 - 1997
Concluído
Graduate Program in Areas of Basic and Appplied Biology (GABBA) (Curso de doutoramento (conclusão de unidades curriculares))
Universidade do Porto, Portugal
Muito bom
1989 - 1994
Concluído
Biologia Vegetal e Aplicada (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
"Detection of p24 antigen for monitoring HIV-1 infection" (TESE/DISSERTAÇÃO)
16
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2024/09/01 - Atual Investigador Contratado (Investigação) Gulbenkian Institute for Molecular Medicine, Portugal
Gulbenkian Institute for Molecular Medicine, Portugal
2018/08/01 - 2024/07/31 Investigador Contratado (Investigação) Instituto de Medicina Molecular, Portugal
Instituto de Medicina Molecular, Portugal
2005/01/01 - 2018/07/31 Pós-doutorado (Investigação) Instituto de Medicina Molecular, Portugal
Instituto de Medicina Molecular, Portugal
2004/01/01 - 2004/12/31 Pós-doutorado (Investigação) Instituto Gulbenkian de Ciência, Portugal
Instituto Gulbenkian de Ciência, Portugal
2003/05/01 - 2003/12/31 Investigador Contratado (Investigação) Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
1995/01/01 - 1996/10/01 Contracted virologist - diagnostics Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
Instituto Nacional de Saúde Doutor Ricardo Jorge, Portugal
Projetos

Bolsa

Designação Financiadores
2017/02/16 - 2018/07/31 Chimeric virus model to investigate the pathogenesis of Kaposi´s sarcoma associated herpesvirus in laboratory mice
IMM/BPD/156-2016
Bolseiro de Pós-Doutoramento
Instituto de Medicina Molecular, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2014/01/01 - 2016/12/31 Development of a mouse model of infection for the investigation of a human oncogenic virus
SFRH/BPD/94786/2013
Bolseiro de Pós-Doutoramento
Instituto de Medicina Molecular, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2011/11/01 - 2013/12/01 Pathogenesis of Kaposi´s sarcoma associated herpesvirus LANA
IMM/BPD/77-2011
Bolseiro de Pós-Doutoramento
Instituto de Medicina Molecular, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2007/06/01 - 2011/10/01 Characterisation of murine gammaherpesvirus proteins involved in the modulation of viral latency
SFRH/BPD/34467/2006
Bolseiro de Pós-Doutoramento
Instituto de Medicina Molecular, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2004/01/01 - 2007/05/01 Analysis of molecular interactions of MHV-68 M2 protein and their role in gammaherpesvirus pathogenesis
SFRH/BPD/14520/2003
Bolseiro de Pós-Doutoramento
Instituto Gulbenkian de Ciência, Portugal

Instituto de Medicina Molecular, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
1998/01/01 - 2002/01/01 Characterisation of Vaccinia Virus C2L
PRAXXIS XXI BD/13774/97
Bolseiro de Doutoramento
University of Oxford, Reino Unido
Fundação para a Ciência e a Tecnologia
Concluído
Produções

Publicações

Artigo em revista
  1. Maria Rebelo; Cong Tang; Ana R Coelho; Carlos Labão-Almeida; Matthias M Schneider; Laurie Tatalick; Pedro Ruivo; et al. "De Novo Human Angiotensin-Converting Enzyme 2 Decoy NL-CVX1 Protects Mice From Severe Disease After Severe Acute Respiratory Syndrome Coronavirus 2 Infection". The Journal of Infectious Diseases (2023): http://dx.doi.org/10.1093/infdis/jiad135.
    10.1093/infdis/jiad135
  2. Mikutis, Sigitas; Rebelo, Maria; Yankova, Eliza; Gu, Muxin; Tang, Cong; Coelho, Ana R.; Yang, Mo; et al. "Proximity-Induced Nucleic Acid Degrader (PINAD) Approach to Targeted RNA Degradation Using Small Molecules". ACS Central Science 9 5 (2023): 892-904. http://dx.doi.org/10.1021/acscentsci.3c00015.
    10.1021/acscentsci.3c00015
  3. Juillard, Franceline; de Miranda, Marta Pires; Li, Shijun; Franco, Aura; Seixas, André F.; Liu, Bing; Álvarez, Ángel L.; et al. "KSHV LANA acetylation-selective acidic domain reader sequence mediates virus persistence". Proceedings of the National Academy of Sciences 117 36 (2020): 22443-22451. http://dx.doi.org/10.1073/pnas.2004809117.
    10.1073/pnas.2004809117
  4. Amado, T.; Amorim, A.; Enguita, F.J.; Romero, P.V.; Inácio, D.; de Miranda, M.P.; Winter, S.J.; et al. "MicroRNA-181a regulates IFN-¿ expression in effector CD8+ T cell differentiation". Journal of Molecular Medicine 98 2 (2020): 309-320. http://www.scopus.com/inward/record.url?eid=2-s2.0-85078721775&partnerID=MN8TOARS.
    10.1007/s00109-019-01865-y
  5. Lawler, C.; de Miranda, M.P.; May, J.; Wyer, O.; Pedro Simas, J.; Stevenson, P.G.. "Gammaherpesvirus colonization of the spleen requires lytic replication in B cells". Journal of Virology 92 7 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85043780062&partnerID=MN8TOARS.
    10.1128/JVI.02199-17
  6. De Miranda, M.P.; Quendera, A.P.; McVey, C.E.; Kaye, K.M.; Simas, J.P.. "In vivo persistence of chimeric virus after substitution of the kaposi's sarcoma-associated herpesvirus LANA DNA binding domain with that of murid herpesvirus 4". Journal of Virology 92 21 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85054897698&partnerID=MN8TOARS.
    10.1128/JVI.01251-18
  7. Habison, A.C.; de Miranda, M.P.; Beauchemin, C.; Tan, M.; Cerqueira, S.A.; Correia, B.; Ponnusamy, R.; et al. "Cross-species conservation of episome maintenance provides a basis for in vivo investigation of Kaposi's sarcoma herpesvirus LANA". PLoS Pathogens 13 9 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85030456472&partnerID=MN8TOARS.
    10.1371/journal.ppat.1006555
  8. Barros-Martins, J.; Schmolka, N.; Fontinha, D.; De Miranda, M.P.; Simas, J.P.; Brok, I.; Ferreira, C.; et al. "Effector ¿d t cell differentiation relies on master but not auxiliary th cell transcription factors". Journal of Immunology 196 9 (2016): 3642-3652. http://www.scopus.com/inward/record.url?eid=2-s2.0-84974815512&partnerID=MN8TOARS.
    10.4049/jimmunol.1501921
  9. Ponnusamy, R.; Petoukhov, M.V.; Correia, B.; Custodio, T.F.; Juillard, F.; Tan, M.; Pires De Miranda, M.; et al. "KSHV but not MHV-68 LANA induces a strong bend upon binding to terminal repeat viral DNA". Nucleic Acids Research 43 20 (2015): 10039-10054. http://www.scopus.com/inward/record.url?eid=2-s2.0-84949978455&partnerID=MN8TOARS.
    10.1093/nar/gkv987
  10. Pires De Miranda, M.; Lopes, F.B.; McVey, C.E.; Bustelo, X.R.; Simas, J.P.. "Role of Src homology domain binding in signaling complexes assembled by the murid ¿-herpesvirus M2 protein". Journal of Biological Chemistry 288 6 (2013): 3858-3870. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873692786&partnerID=MN8TOARS.
    10.1074/jbc.M112.439810
  11. Correia, B.; Cerqueira, S.A.; Beauchemin, C.; Pires de Miranda, M.; Li, S.; Ponnusamy, R.; Rodrigues, L.; et al. "Crystal Structure of the Gamma-2 Herpesvirus LANA DNA Binding Domain Identifies Charged Surface Residues Which Impact Viral Latency". PLoS Pathogens 9 10 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84887309755&partnerID=MN8TOARS.
    10.1371/journal.ppat.1003673
  12. de Miranda, M.P.; Alenquer, M.; Marques, S.; Rodrigues, L.; Lopes, F.; Bustelo, X.R.; Simas, J.P.. "The gammaherpesvirus m2 protein manipulates the Fyn/Vav pathway through a multidocking mechanism of assembly". PLoS ONE 3 2 (2008): http://www.scopus.com/inward/record.url?eid=2-s2.0-45749086951&partnerID=MN8TOARS.
    10.1371/journal.pone.0001654
  13. Pires de Miranda, M.; Carmo Gomes, M.; Rebelo de Andrade, H.. "Seroprevalence of antibodies to poliovirus in individuals living in Portugal, 2002.". Euro surveillance : bulletin européen sur les maladies transmissibles = European communicable disease bulletin 12 6 (2007): http://www.scopus.com/inward/record.url?eid=2-s2.0-38449105786&partnerID=MN8TOARS.
  14. Rodrigues, L.; Pires De Miranda, M.; Caloca, M.J.; Bustelo, X.R.; Simas, J.P.. "Activation of Vav by the gammaherpesvirus M2 protein contributes to the establishment of viral latency in B lymphocytes". Journal of Virology 80 12 (2006): 6123-6135. http://www.scopus.com/inward/record.url?eid=2-s2.0-33744929125&partnerID=MN8TOARS.
    10.1128/JVI.02700-05
Tese / Dissertação
  1. Pires de Miranda, Marta. "Studies on the vaccinia virus C2L protein: a member of the kelch family of proteins". Doutoramento, University of Oxford, 2002.
Atividades

Orientação

Título / Tema
Papel desempenhado
Curso (Tipo)
Instituição / Organização
2011/04/01 - 2015/03/31 Structural Virology of the murid Gamma-Herpesvirus 4 (MuHV-4) - Elucidating the roles of viral latency proteins M2 and ORF73.
Coorientador
Structural biology (Doutoramento)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
1997 - Atual Practical class within Virology module, degree in Biologia Microbiana e Genética, University of Lisbon - Faculdade de Ciências Biologia Microbiana e Genética (Licenciatura) Universidade de Lisboa Faculdade de Ciências, Portugal
2014 - 2015 Organizer and demonstrator of four practical classes, Masters in Microbiology, Universidade de Lisboa - Faculdade de Medicina (Mestrado) Universidade de Lisboa Faculdade de Medicina, Portugal
2005 - 2007 Organizer and demonstrator of several practical classes, MSc in Infectious Diseases and BSc in Microbiology, Universidade de Lisboa - Faculdade de Medicina (Licenciatura) Universidade de Lisboa Faculdade de Medicina, Portugal
2000 - 2000 Demonstrator, Virology Practicals, Medical students, University of Oxford - Sir William Dunn School of Pathology (Bachelor) University of Oxford - Sir William Dunn School of Pathology, Portugal

Tutoria

Tópico Nome do aluno
2021/01 - 2021/10 Expression and purification of proteins from E. coli. (BSc graduate, internship). André Ventura Gomes
2019/09 - 2020/09 Expression and purification of viral proteins for vaccination (work placement BSc Genetics, University of Leeds) Beatriz Arede
2018/02 - 2018/06 Caracterização de soros policlonais de ratinho contra LANA, proteína codificada pelo vírus herpes associado ao sarcoma de Kaposi (BSc Project) Ana Sofia Marques
2018/01/01 - 2018/04/01 Immunofluorescence for kLANA and mLANA detection (BSc Project) Joana Jesus
2016/09 - 2017/09 Utilization of a mouse model of infection to investigate Kaposi´s sarcoma-associated herpesvirus LANA function in vivo (MSc thesis) Aura Franco
2016/09 - 2017/09 Using chimeric viruses to study Kaposi´s sarcoma-associated herpesvirus pathogenesis in mice (MSc thesis) Kateryna Kholod
2015/09 - 2016/09 Using chimeric viruses to study Kaposi´s sarcoma-associated herpesvirus pathogenesis in mice (MSc thesis) André Filipe Seixas
2015/05 - 2016/05 Investigation of Kaposi´s Sarcoma-associated herpesvirus in vivo (MSc research assistant) Ana Patrícia Quendera