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Identificação

Identificação pessoal

Nome completo
Antonio Roldao

Nomes de citação

  • Roldao, Antonio

Identificadores de autor

Ciência ID
CB1F-B0AA-9F18
ORCID iD
0000-0003-0414-4899
Researcher Id
J-2396-2013
Scopus Author Id
8892062600

Websites

Domínios de atuação

  • Ciências da Engenharia e Tecnologias - Biotecnologia Industrial
  • Ciências da Engenharia e Tecnologias - Biotecnologia Industrial - Biocombustíveis
  • Ciências da Engenharia e Tecnologias - Biotecnologia Industrial - Tecnologias de BioProcessamento
  • Ciências Médicas e da Saúde - Biotecnologia Médica - Biotecnologia Aplicada à Saúde
  • Ciências da Engenharia e Tecnologias - Engenharia Química - Engenharia dos Processos Químicos
  • Ciências da Engenharia e Tecnologias - Engenharia Química - Engenharia Química

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2)
Inglês Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2) Utilizador proficiente (C2)
Formação
Grau Classificação
2010/06/18
Concluído
 Ciências da Engenharia e Tecnologia (Doutoramento)
Especialização em Especialidade: Biologia de Sistemas
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
"Production Optimization of Rotavirusl-like Particles: a Systems Biology Approach" (TESE/DISSERTAÇÃO)
1997 - 2003
Concluído
 Chemical Engineering (Licenciatura)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Percurso profissional

Outros

Categoria Profissional
Instituição de acolhimento 		Empregador
2021/01/01 - Atual Head of Late-stage R&D Bioproduction Unit Instituto de Biologia Experimental e Tecnológica, Portugal
2021/01/01 - Atual Coordinator of Late-Stage R&D Bioproduction Unit Instituto de Biologia Experimental e Tecnológica, Portugal
2019/01/14 - Atual Head of Cell Based Vaccines Development Lab, Animal Cell Technology Unit Instituto de Biologia Experimental e Tecnológica, Portugal
2015 - Atual Senior researcher, iNOVA4Health – Advancing Precision Medicine (Portugal) Instituto de Biologia Experimental e Tecnológica, Portugal
2014/06/01 - 2020/06/01 Investigador FCT Instituto de Biologia Experimental e Tecnológica, Portugal
2010 - 2014 Post-Doc and Director of Fermentation Infrastructure, Systems and Synthetic Biology group Chalmers tekniska högskola, Suécia
2006 - 2010 PhD Student Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2009 - 2009 Bioprocess Engineer GenIBET, Portugal
2004 - 2006 Junior researcher Instituto de Biologia Experimental e Tecnológica, Portugal
2003 - 2004 Junior researcher Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Projetos

Bolsa

Designação Financiadores
2023/09/01 - 2025/08/31 Towards the clinical development of the new asexual blood-stage malaria vaccine candidate PfRipr5
PfRipr5-PD
Investigador responsável
Instituto de Biologia Experimental e Tecnológica, Portugal
 Global Health Innovative Technology Fund
Em curso
2020/10 - 2024/09 ADDovenom: Novel Snakebite Therapy Platform of Unparalleled Efficacy, Safety and Affordability
899670
Investigador responsável
European Commission
2020 - 2024 TRANSVAC-DS – Design Study for a European Vaccine Infrastructure
951668
Instituto de Biologia Experimental e Tecnológica, Portugal
 European Commission
Em curso
2019 - 2023 CRUZIVAX
815418
Investigador responsável
European Commission
2019 - 2023 ProMeTeus
265558
Investigador responsável
European Commission
2019 - 2023 STACCATO
813453
Investigador responsável
European Commission
2018/08 - 2021/08 Multivalent inactivated vaccine against heartwater in Africa – MuVHA
ANR-18-LEAP-0005
Investigador responsável
Agence nationale de la recherche
2019 - 2021 Further development of a new asexual blood-stage malaria vaccine candidate
T2018-151
Investigador responsável
Global Health Innovative Technology Fund
2018 - 2021 FIGHT-TWO
PTDC/CVT-CVT/29062/2017-PT2020
Investigador responsável
Governo da República Portuguesa Ministério da Ciência Tecnologia e Ensino Superior
2018 - 2021 MetaCardio: Metabolic signatures of human pluripotent stem cells derived cardiomyocytes for improved cell therapy
LISBOA-01-0145-FEDER-032566
Investigador
Ministério da Educação
2017 - 2021 TRANSVAC2
228403
Investigador responsável
European Commission
2015 - 2020 Next Generation Seasonal Influenza Vaccines: Towards a VLP-based Production Platform
IF/01704/2014/CP1229/CT0001
Investigador responsável
Instituto de Biologia Experimental e Tecnológica, Portugal
 Fundação para a Ciência e a Tecnologia
Em curso
2018 - 2019 Desenvolvimento e implementação de medidas práticas impulsionadoras da recuperação dos leporídeos silvestres em portugal
+Coelho2
Investigador
Portuguese Ministry of Agriculture, Forestry and Rural Development
2013 - 2017 EDUFLUVAC
602640
Investigador responsável
European Commission
2013 - 2015 Renewable Energy: an advanced Bio-Platform for 1-Butanol Production in Yeast
n
Investigador responsável
J. Gust. Richert Foundation
2012 - 2014 Integrated Bioprocess Engineering Platform for Biobutanol Production
n.a.
Investigador responsável
ÅForsk
2010/04 - 2013/06 Novel Cell Factories for the production of secreted complex bioproducts: a syntetic biology approach for improved product stoichiometries
grant.Provided by PTCRIS: 102266
Bolseiro de Pós-Doutoramento
Stichting Weten
2010/03 - 2013/05 2D fluorometry: a powerful tool to improve mammalian cell process development
grant.Provided by PTCRIS: 102750
Bolseiro de Pós-Doutoramento
Stichting Weten
2010/03 - 2013/02 BACULOME-Engineering cellular energetics for improvement of bioprocesses: metabolic modeling for enhanced vaccines production.
Provided by PTCRIS: 103359
Bolseiro de Pós-Doutoramento
Fundação para a Ciência e a Tecnologia
2008/12 - 2011/11 Systems Biology as a Driver for Industrial Biotechnology
212766
Bolseiro de Integração na Investigação
European Commission
2005/01 - 2009/12 Development of marker vaccines, companion diagnostic tests and improvement of epidemiological knowledge to facilitate control of rinderpest and peste des petits ruminants viruses
3670
Bolseiro de Integração na Investigação
European Commission
2005/07 - 2009/06 Epidemiology and new generation vaccines for Ehrlichia and anaplasma infections of ruminants
3713
Bolseiro de Integração na Investigação
European Commission
2006 - 2009 BACULOGENES – Baculovirus Vectors for Gene Therapy
37541
Bolseiro de Integração na Investigação
European Commission
2005/05 - 2008/06 THERAVECT - Production, purification and storage of retroviral vectors produced by modular master producer cells: focus on CoIVII vectors for the Epidermolysis Bullosa therapy
Provided by PTCRIS: 63789
Bolseiro de Integração na Investigação
Fundação para a Ciência e a Tecnologia
2005/05 - 2007/12 Genetics, Stoicheometry and Kinetics of Multiple Protein Assembly: the case for a Rotavirus Like Particle
grant.Provided by PTCRIS: 55975
grant.Provided by PTCRIS: 82520
Bolseiro de Doutoramento
Stichting Weten
2003 - 2005 Novel strategies for a safe rotavirus vaccine
QLRT-2001-01249
Bolseiro de Integração na Investigação
European Commission

Projeto

Designação Financiadores
2023 - Atual Production of recombinant proteins
n.a.
Co-Investigador Responsável (Co-IR)
Concluído
2023 - Atual Production of virus-based vaccine
n.a.
Co-Investigador Responsável (Co-IR)
Em curso
2022 - Atual Production of viral vector
n.a.
Co-Investigador Responsável (Co-IR)
Em curso
2022 - Atual Production of viral vector
n.a.
Co-Investigador Responsável (Co-IR)
Concluído
2022 - Atual Production of viral vector
n.a.
Co-Investigador Responsável (Co-IR)
Em curso
2022 - Atual Production of viral vector
n.a.
Co-Investigador Responsável (Co-IR)
Concluído
2021 - Atual Production of viral vector
n.a.
Co-Investigador Responsável (Co-IR)
Concluído
2021 - Atual Production of recombinant proteins
n.a.
Co-Investigador Responsável (Co-IR)
Concluído
2021 - Atual Production of recombinant proteins and viral vector
n.a.
Co-Investigador Responsável (Co-IR)
Concluído
2021 - Atual Production of recombinant proteins
n.a.
Co-Investigador Responsável (Co-IR)
Em curso
2021 - Atual Production of recombinant proteins
n.a.
Co-Investigador Responsável (Co-IR)
Em curso
2019 - Atual Process development for Turnstone Biologics
n.a.
Co-Investigador Responsável (Co-IR)
Concluído
2016 - Atual Production of recombinant AAVs in insect cells
n.a.
Co-Investigador Responsável (Co-IR)
Concluído
2015 - 2020 Investigador FCT
n.a.
Investigador responsável
Instituto de Biologia Experimental e Tecnológica, Portugal
 Fundação para a Ciência e a Tecnologia
Em curso
2018 - 2018 Metabolic flux analysis (MFA) of insect Sf9 cell cultures producing AAV vectors
n.a.
Investigador responsável
Concluído
2018 - 2018 Beta testing of a chemically defined culture medium for insect cells
n.a.
Investigador responsável
Sartorius AG
Concluído
2017 - 2017 Elucidating the engineering principles in small-scale bioreactor systems for rational process intensification
n.a.
Investigador responsável
Instituto de Biologia Experimental e Tecnológica
Concluído
2015 - 2015 Production of Dengue VLPs in Sf9-RMCE cell line
n.a.
Investigador responsável
Concluído
2015 - 2015 Production of Intracellular Proteins using IC-BEVS
n.a.
Investigador responsável
Redbiotec AG
Concluído
Produções

Publicações

Artigo em revista
  1. Stefanie Menzies; Raquel Arinto-Garcia; Fernanda Gobbi Amorim; Iara Aimê Cardoso; Camille Abada; Thomas Crasset; Fabien Durbesson; et al. "ADDovenom: Thermostable Protein-Based ADDomer Nanoparticles as New Therapeutics for Snakebite Envenoming". Toxins (2023): https://www.mdpi.com/2072-6651/15/12/673.
    10.3390/toxins15120673
  2. Mooij, Petra; Mortier, Daniella; Aartse, Aafke; Murad, Alexandre B.; Correia, Ricardo; Roldão, António; Alves, Paula M.; et al. "Vaccine-induced neutralizing antibody responses to seasonal influenza virus H1N1 strains are not enhanced during subsequent pandemic H1N1 infection". Frontiers in Immunology 14 (2023): http://dx.doi.org/10.3389/fimmu.2023.1256094.
    10.3389/fimmu.2023.1256094
  3. Geada, Isabelle; Roldão, Antonio; Betenbaugh, Michael J.; Alves, Paula M.. "Advanced cell technologies: Making protein, cell, and gene therapies a reality". Biotechnology and Bioengineering 120 9 (2023): 2385-2388. http://dx.doi.org/10.1002/bit.28521.
    10.1002/bit.28521
  4. Escandell, José; Moura, Filipa; Carvalho, Sofia B.; Silva, Ricardo J. S.; Correia, Ricardo; Roldão, Antonio; Gomes-Alves, Patrícia; Alves, Paula M.. "Towards a scalable bioprocess for rAAV production using a HeLa stable cell line". Biotechnology and Bioengineering 120 9 (2023): 2578-2587. http://dx.doi.org/10.1002/bit.28394.
    10.1002/bit.28394
  5. Virgolini, Nikolaus; Silvano, Marco; Hagan, Ryan; Correia, Ricardo; Alves, Paula M.; Clarke, Colin; Roldão, António; Isidro, Inês A.. "Impact of dual baculovirus infection on the Sf9 insect cell transcriptome during AAV production using single-cell RNA-seq". Biotechnology and Bioengineering (2023): http://dx.doi.org/10.1002/bit.28377.
    10.1002/bit.28377
  6. Silvano, Marco; Virgolini, Nikolaus; Correia, Ricardo; Clarke, Colin; Isidro, Inês A.; Alves, Paula M.; Roldão, António. "Dissecting insect cell heterogeneity during influenza VLP production using single-cell transcriptomics". Frontiers in Bioengineering and Biotechnology 11 (2023): http://dx.doi.org/10.3389/fbioe.2023.1143255.
    10.3389/fbioe.2023.1143255
  7. Virgolini, Nikolaus; Hagan, Ryan; Correia, Ricardo; Silvano, Marco; Fernandes, Sofia; Alves, Paula M.; Clarke, Colin; Roldão, António; Isidro, Inês A.. "Transcriptome analysis of Sf9 insect cells during production of recombinant Adeno-associated virus". Biotechnology Journal 18 2 (2022): 2200466. http://dx.doi.org/10.1002/biot.202200466.
    10.1002/biot.202200466
  8. Mendes, João P.; Fernandes, Bárbara; Pineda, Earl; Kudugunti, Shashi; Bransby, Mike; Gantier, René; Peixoto, Cristina; et al. "AAV process intensification by perfusion bioreaction and integrated clarification". Frontiers in Bioengineering and Biotechnology 10 (2022): http://dx.doi.org/10.3389/fbioe.2022.1020174.
    10.3389/fbioe.2022.1020174
  9. Alexandre B. Murad; Marcos Q. Sousa; Ricardo Correia; Inês A. Isidro; Manuel J. T. Carrondo; António Roldão. "Targeted Metabolic Analysis and MFA of Insect Cells Expressing Influenza HA-VLP". Processes (2022): https://doi.org/10.3390/pr10112283.
    10.3390/pr10112283
  10. Takashima, Eizo; Nagaoka, Hikaru; Correia, Ricardo; Alves, Paula M.; Roldão, António; Christensen, Dennis; Guderian, Jeffrey A.; et al. "A novel asexual blood-stage malaria vaccine candidate: PfRipr5 formulated with human-use adjuvants induces potent growth inhibitory antibodies". Frontiers in Immunology 13 (2022): http://dx.doi.org/10.3389/fimmu.2022.1002430.
    10.3389/fimmu.2022.1002430
  11. Marco Silvano; Ricardo Correia; Nikolaus Virgolini; Colin Clarke; Paula M. Alves; Inês A. Isidro; António Roldão. "Gene Expression Analysis of Adapted Insect Cells during Influenza VLP Production Using RNA-Sequencing". Viruses (2022): https://doi.org/10.3390/v14102238.
    10.3390/v14102238
  12. Correia, Ricardo; Fernandes, Bárbara; Castro, Rute; Nagaoka, Hikaru; Takashima, Eizo; Tsuboi, Takafumi; Fukushima, Akihisa; et al. "Asexual Blood-Stage Malaria Vaccine Candidate PfRipr5: Enhanced Production in Insect Cells". Frontiers in Bioengineering and Biotechnology 10 (2022): http://dx.doi.org/10.3389/fbioe.2022.908509.
    10.3389/fbioe.2022.908509
  13. Fernandes, Bárbara; Correia, Ricardo; Alves, Paula M.; Roldão, António. "Intensifying Continuous Production of Gag-HA VLPs at High Cell Density Using Stable Insect Cells Adapted to Low Culture Temperature". Frontiers in Bioengineering and Biotechnology 10 (2022): http://dx.doi.org/10.3389/fbioe.2022.917746.
    10.3389/fbioe.2022.917746
  14. Fernandes, Bárbara; Sousa, Marcos; Castro, Rute; Schäfer, Anja; Hauser, Julia; Schulze, Kai; Amacker, Mario; et al. "Scalable Process for High-Yield Production of PfCyRPA Using Insect Cells for Inclusion in a Malaria Virosome-Based Vaccine Candidate". Frontiers in Bioengineering and Biotechnology 10 (2022): http://dx.doi.org/10.3389/fbioe.2022.879078.
    10.3389/fbioe.2022.879078
  15. Bárbara Dias Fernandes; Rute Castro; Farien Bhoelan; Denzel Bemelman; Gomes, R.A.; Julia Costa; Patrícia Gomes-Alves; et al. "Insect Cells for High-Yield Production of SARS-CoV-2 Spike Protein: Building a Virosome-Based COVID-19 Vaccine Candidate". Pharmaceutics (2022): https://www.mdpi.com/1999-4923/14/4/854.
    10.3390/pharmaceutics14040854
  16. "Bioanalytics for Influenza Virus-Like Particle Characterization and Process Monitoring". Frontiers in Bioengineering and Biotechnology 10 (2022): http://dx.doi.org/10.3389/fbioe.2022.805176.
    10.3389/fbioe.2022.805176
  17. "Functionalizing Ferritin Nanoparticles for Vaccine Development". Pharmaceutics 13 10 (2021): 1621-1621. http://dx.doi.org/10.3390/pharmaceutics13101621.
    10.3390/pharmaceutics13101621
  18. "Insect High Five™ cell line development using site-specific flipase recombination technology". G3 Genes|Genomes|Genetics 11 8 (2021): http://dx.doi.org/10.1093/g3journal/jkab166.
    10.1093/g3journal/jkab166
  19. Bárbara Fernandes; Ricardo Correia; Marcos Sousa; Manuel J. T. Carrondo; Paula M. Alves; António Roldão. "Integrating high cell density cultures with adapted laboratory evolution for improved Gag-HA virus-like particles production in stable insect cell lines". Biotechnology and Bioengineering (2021): https://doi.org/10.1002/bit.27766.
    10.1002/bit.27766
  20. Ricardo Correia; Liane Meneses; Carolina Richheimer; Paula M. Alves; Manuel J.T. Carrondo; Ana Rita C. Duarte; Alexandre Paiva; António Roldão. "Improved storage of influenza HA-VLPs using a trehalose-glycerol natural deep eutectic solvent system". Vaccine 39 24 (2021): 3279-3286. https://doi.org/10.1016/j.vaccine.2021.04.033.
    10.1016/j.vaccine.2021.04.033
  21. Sousa, B.B.; Sousa, M.F.Q.; Marques, M.C.; Seixas, J.D.; Brito, J.A.; Matias, P.M.; Bernardes, G.J.L.; Roldão, A.. "A scalable insect cell-based production process of the human recombinant BMX for in-vitro covalent ligand high-throughput screening". Bioprocess and Biosystems Engineering 44 1 (2021): 209-215. http://www.scopus.com/inward/record.url?eid=2-s2.0-85089656524&partnerID=MN8TOARS.
    10.1007/s00449-020-02421-6
  22. Bárbara Fernandes; João Vidigal; Ricardo Correia; Manuel J.T. Carrondo; Paula M. Alves; Ana P. Teixeira; António Roldão. "Adaptive laboratory evolution of stable insect cell lines for improved HIV-Gag VLPs production". Journal of Biotechnology 307 (2020): 139-147. https://doi.org/10.1016/j.jbiotec.2019.10.004.
    10.1016/j.jbiotec.2019.10.004
  23. Correia, R.; Fernandes, B.; Alves, P.M.; Carrondo, M.J.T.; Roldão, A.. "Improving influenza HA-VLPs production in insect high five cells via adaptive laboratory evolution". Vaccines 8 4 (2020): 1-15. http://www.scopus.com/inward/record.url?eid=2-s2.0-85092491644&partnerID=MN8TOARS.
    10.3390/vaccines8040589
  24. Marcos Sousa; Christel Fenge; Jens Rupprecht; Alexander Tappe; Gerhard Greller; Paula Alves; Manuel Carrondo; António Roldão. "Process intensification for Peste des Petites Ruminants Virus vaccine production". Vaccine 37 47 (2019): 7041-7051. https://doi.org/10.1016/j.vaccine.2019.07.009.
    10.1016/j.vaccine.2019.07.009
  25. Hayley K. Charlton Hume; João Vidigal; Manuel J. T. Carrondo; Anton P. J. Middelberg; António Roldão; Linda H. L. Lua. "Synthetic biology for bioengineering virus-like particle vaccines". Biotechnology and Bioengineering (2018): https://doi.org/10.1002/bit.26890.
    10.1002/bit.26890
  26. Vidigal, J.; Fernandes, B.; Dias, M.M.; Patrone, M.; Roldão, A.; Carrondo, M.J.T.; Alves, P.M.; Teixeira, A.P.. "RMCE-based insect cell platform to produce membrane proteins captured on HIV-1 Gag virus-like particles". Applied Microbiology and Biotechnology (2017): 1-12. http://www.scopus.com/inward/record.url?eid=2-s2.0-85034256685&partnerID=MN8TOARS.
    10.1007/s00253-017-8628-3
  27. Sequeira, D.P.; Correia, R.; Carrondo, M.J.T.; Roldão, A.; Teixeira, A.P.; Alves, P.M.. "Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production". Vaccine (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85014803559&partnerID=MN8TOARS.
    10.1016/j.vaccine.2017.02.043
  28. Sousa, M.F.Q.; Silva, M.M.; Giroux, D.; Hashimura, Y.; Wesselschmidt, R.; Lee, B.; Roldão, A.; et al. "Production of oncolytic adenovirus and human mesenchymal stem cells in a single-use, Vertical-Wheel bioreactor system: Impact of bioreactor design on performance of microcarrier-based cell culture processes". Biotechnology Progress 31 6 (2015): 1600-1612. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956595618&partnerID=MN8TOARS.
    10.1002/btpr.2158
  29. Kim, I.-K.; Roldão, A.; Siewers, V.; Nielsen, J.. "A systems-level approach for metabolic engineering of yeast cell factories". FEMS Yeast Research 12 2 (2012): 228-248. http://www.scopus.com/inward/record.url?eid=2-s2.0-84857058761&partnerID=MN8TOARS.
    10.1111/j.1567-1364.2011.00779.x
  30. Roldão, A.; Mellado, M.C.M.; Lima, J.C.; Carrondo, M.J.T.; Alves, P.M.; Oliveira, R.. "On the effect of thermodynamic equilibrium on the assembly efficiency of complex multi-layered virus-like particles (VLP): The case of rotavirus VLP". PLoS Computational Biology 8 2 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84861122841&partnerID=MN8TOARS.
    10.1371/journal.pcbi.1002367
  31. Vicente, T.; Roldão, A.; Peixoto, C.; Carrondo, M.J.T.; Alves, P.M.. "Large-scale production and purification of VLP-based vaccines". Journal of Invertebrate Pathology 107 SUPPL. (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-79960620240&partnerID=MN8TOARS.
    10.1016/j.jip.2011.05.004
  32. Roldão, A.; Vicente, T.; Peixoto, C.; Carrondo, M.J.T.; Alves, P.M.. "Quality control and analytical methods for baculovirus-based products". Journal of Invertebrate Pathology 107 SUPPL. (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-79960636554&partnerID=MN8TOARS.
    10.1016/j.jip.2011.05.009
  33. Roldão, A.; Mellado, M.C.M.; Castilho, L.R.; Carrondo, M.J.T.; Alves, P.M.. "Virus-like particles in vaccine development". Expert Review of Vaccines 9 10 (2010): 1149-1176. http://www.scopus.com/inward/record.url?eid=2-s2.0-77957936654&partnerID=MN8TOARS.
    10.1586/erv.10.115
  34. Roldao, Antonio. "In silico rotavirus-like particle production in the recombinant baculovirus/insect cell system". New Biotechnology (2009): http://dx.doi.org/10.1016/j.nbt.2009.06.861.
    10.1016/j.nbt.2009.06.861
  35. Roldão, A.; Oliveira, R.; Carrondo, M.J.T.; Alves, P.M.. "Error assessment in recombinant baculovirus titration: Evaluation of different methods". Journal of Virological Methods 159 1 (2009): 69-80. http://www.scopus.com/inward/record.url?eid=2-s2.0-67349134657&partnerID=MN8TOARS.
    10.1016/j.jviromet.2009.03.007
  36. Roldão, A.; Carrondo, M.J.T.; Alves, P.M.; Oliveira, R.. "Stochastic simulation of protein expression in the baculovirus/insect cells system". Computers and Chemical Engineering 32 1-2 (2008): 68-77. http://www.scopus.com/inward/record.url?eid=2-s2.0-35548954557&partnerID=MN8TOARS.
    10.1016/j.compchemeng.2007.04.017
  37. Marcelino, I.; Vachiéry, N.; Amaral, A.I.; Roldão, A.; Lefrançois, T.; Carrondo, M.J.T.; Alves, P.M.; Martinez, D.. "Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater". Vaccine 25 26 (2007): 4903-4913. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249824277&partnerID=MN8TOARS.
    10.1016/j.vaccine.2007.04.055
  38. Roldão, A.; Vieira, H.L.A.; Charpilienne, A.; Poncet, D.; Roy, P.; Carrondo, M.J.T.; Alves, P.M.; Oliveira, R.. "Modeling rotavirus-like particles production in a baculovirus expression vector system: Infection kinetics, baculovirus DNA replication, mRNA synthesis and protein production". Journal of Biotechnology 128 4 (2007): 875-894. http://www.scopus.com/inward/record.url?eid=2-s2.0-33847390211&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2007.01.003
  39. Roldão, A.; Vieira, H.L.A.; Alves, P.M.; Oliveira, R.; Carrondo, M.J.T.. "Intracellular dynamics in rotavirus-like particles production: Evaluation of multigene and monocistronic infection strategies". Process Biochemistry 41 10 (2006): 2188-2199. http://www.scopus.com/inward/record.url?eid=2-s2.0-33748567742&partnerID=MN8TOARS.
    10.1016/j.procbio.2006.06.019
  40. Rodrigues, T.; Carvalho, A.; Roldão, A.; Carrondo, M.J.T.; Alves, P.M.; Cruz, P.E.. "Screening anion-exchange chromatographic matrices for isolation of onco-retroviral vectors". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 837 1-2 (2006): 59-68. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646896734&partnerID=MN8TOARS.
    10.1016/j.jchromb.2006.03.061
  41. Coroadinha, A.S.; Ribeiro, J.; Roldão, A.; Cruz, P.E.; Alves, P.M.; Merten, O.-W.; Carrondo, M.J.T.. "Effect of medium sugar source on the production of retroviral vectors for gene therapy". Biotechnology and Bioengineering 94 1 (2006): 24-36. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646551820&partnerID=MN8TOARS.
    10.1002/bit.20778
  42. Cruz, P.E.; Silva, A.C.; Roldão, A.; Carmo, M.; Carrondo, M.J.T.; Alves, P.M.. "Screening of novel excipients for improving the stability of retroviral and adenoviral vectors". Biotechnology Progress 22 2 (2006): 568-576. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646066281&partnerID=MN8TOARS.
    10.1021/bp050294y
  43. Vieira, H.L.A.; Estêvão, C.; Roldão, A.; Peixoto, C.C.; Sousa, M.F.Q.; Cruz, P.E.; Carrondo, M.J.T.; Alves, P.M.. "Triple layered rotavirus VLP production: Kinetics of vector replication, mRNA stability and recombinant protein production". Journal of Biotechnology 120 1 (2005): 72-82. http://www.scopus.com/inward/record.url?eid=2-s2.0-25644448228&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2005.03.026
Capítulo de livro
  1. Roldão, A.; Silva, A.C.; Mellado, M.C.M.; Alves, P.M.; Carrondo, M.J.T.. "Viruses and virus-like particles in biotechnology: Fundamentals and applications". 633-656. 2019.
    10.1016/B978-0-08-088504-9.00072-6
  2. Roldao, Antonio; Roldão, A.; Vieira, H.L.A.; Carrondo, M.J.T.; Alves, P.M.; Oliveira, R.. "Rotavirus-like particle production: Simulation of protein production and particle assembly". In Computer Aided Chemical Engineering, 1673-1678. 2006.
    10.1016/s1570-7946(06)80288-9
Livro
  1. Roldão, A.; Cox, M.; Alves, P.; Carrondo, M.; Vicente, T.. Industrial Large Scale of Suspension Culture of Insect Cells. 2014.
    10.1002/9783527683321.ch10
  2. Roldão, A.; Kim, I.-K.; Nielsen, J.. Bridging omics technologies with synthetic biology in yeast industrial biotechnology. 2012.
    10.1007/978-94-007-4534-6_9
Tese / Dissertação
  1. SEQUEIRA, Daniela Filipa Policarpo. "Exploring insect cells versatility for production of influenza virus-like particles". Mestrado, 2015. http://hdl.handle.net/10362/19341.
  2. Roldão, António Manuel Missionário. "Production optimization of rotavirus-like particles: a system biology approach". Doutoramento, 2010. http://hdl.handle.net/10362/5246.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2024/02/05 Enhancing rAAV biomanufacturing: Process intensification strategies for streamlined upstream and downstream processing Advancing Manufacture of Cell and Gene Therapies VIII
2023/12/06 Continuous production of influenza VLPs as vaccine candidates: a multi-stage bioreactor approach MicroBiotec23
2023/06/09 Leveraging single-cell transcriptomics to tailor insect cells for production of rAAV and influenza VLPs 16th PEACe - Protein Expression in Animal Cells Conference
2023 Single cell and Bulk transcriptomics of insect cells during production of AAV and influenza HA-VLPs ISBioTech 12th Spring Meeting,
(Norfolk, Estados Unidos)
2022 Influenza VLP Vaccine Production Done Better: The Perks of Innovating Upstream, Downstream, Storage, and Analytics ISBioTech 11th Spring Meeting
2022 Advances in bioprocessing and analytics to accelerate vaccine production Analytical Technologies in the Biopharmaceutical Industry
(Lisboa, Portugal)
2022 Advances in bioprocessing, analytics and formulation of influenza HA-VLP vaccine candidates produced by insect cells Vaccine Technology VIII
(Sitges, Espanha)
2022 The perks of innovating influenza VLP-based vaccine production 27th ESACT Meeting
(Lisboa, Portugal)
2021 Produção de mRNA e vacinas 2º Encontro de ATMPs | SPFCS
2021 Bioprocessos para a produção de vacinas V Ciclo de Conferências em Biotecnologia
2021 Advances in bioprocessing and analytics to accelerate vaccine production ESBES Virtual – Vaccine production in the light of COVID19 pandemic
2021 Vacinas: do desenho conceptual à produção FCUL – Mestrado de Biologia Molecular e Genética
2021 Vaccines: From the conceptual design to manufacturing VI Jornadas de Bioengenharia – UTAD
2021 Como são produzidas as vacinas? SCIENCE 4 ALL - Vacinas: da investigação e produção à imunidade
2021 Contribution of iBET to PPRV vaccines: From production & purification to vaccine stability PPR-GREN - THERMOTOLERANT VACCINE MEETING
2021 Bioprocessos para a produção de vacinas XIV Congresso Científico NEF/AAC
2021 Advances in bioprocessing and analytics to accelerate vaccine production EncontroCIÊNCIA´21
2020 Como são produzidas as vacinas? (2020) Infarmed
(Lisboa, Portugal)
2020 Advances in bioprocessing and analytics to accelerate vaccine production JAACT 2020
online
2020 Integrating High Cell Density cultures with Adapted Laboratory Evolution for vaccine production using stable insect cell lines PEGS Europe Virtual
2020 Vaccine process development: From the conceptual design to manufacturing AICIB/EATRIS/PtCRIN Joint Initiative
2019 Produção de vacinas para aplicação em saúde animal: da molécula ao isco Wildlife & Game Management Innovation Summit 2019
INIAV (Oeiras, Portugal)
2019 Bioprocess Engineering of Insect Cells for Enhanced Gag-VLPs Production Bioprocessing Summit Europe: Bioproduction: Scale, Bioreactors and Disposables
(Lisboa, Portugal)
2019 Vaccines: DNA, mRNA, recombinant proteins and virus-like particles 8th International School on Production of Biologicals
(Rio de Janeiro, Brasil)
2018 Bioprocess Engineering of Insect Cells for Pseudotyped VLP Expression and Optimization ISBioTech 8th Spring Meeting
(Norfolk, Estados Unidos)
2018 Insect cell platforms for production of pseudo-typed VLPs for drug and vaccine development Cell Culture Engineering XVI
(Tampa, Estados Unidos)
2018 Bioprocess intensification for production of a Peste des petits ruminants virus (PPRV) vaccine Vaccine Technology VII
(Mont Tremblant, Canadá)
2017 Bioprocess Engineering of Insect Cells for Accelerating Vaccines Development 9th Annual PEGS Europe: Protein & Antibody Engineering Summit
(Lisboa, Portugal)
2016 Insect cells platform for fast production of pseudo-typed VLPs Vaccine Technology VI
(Albufeira, Portugal)
2016 Lessons learnt on producing and purifying over thirty different VLP«s for a universal influenza vaccine World Vaccine Congress Europe
(Barcelona, Espanha)
2016 Universal Influenza VLP-based vaccine: What can we learn from producing over thirty different VLPs? 10th Vaccine Congress
(Amsterdam, Países Baixos)
2016 Combining stable and baculovirus-mediated expression towards production of a “universal” influenza VLP-based vaccine Virus-Like Particle & Nano-Particle Vaccines
(Leiden, Países Baixos)

Orientação

Título / Tema
Papel desempenhado 		Curso (Tipo)
Instituição / Organização
2024/02/01 - Atual Process optimisation of functionalised Adenovirus-like particle (ADDomer) to use as a snakebite therapy
Orientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2024/02/01 - Atual Optimization of Polio virus-like particles production and purification processes
Orientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2024/02/01 - Atual Optimizing downstream processing for efficient ferritin-based vaccine production
Orientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2023 - Atual Continuous integrated production of Adenovirus-like particles (ADDomer)
Coorientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2022 - Atual Bioprocess optimization of a ferritin-based vaccine candidate against Rift Valley Fever
Orientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2022 - Atual Continuous production of influenza virus like particles using the insect cell baculovirus expression vector system
Orientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2021 - Atual Rational genetic engineering of insect cells to improve rAAVs and influenza VLPs production yield utilizing the baculovirus expression vector system
Orientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2021 - Atual Establishment of an insect cell-based platform for production of Adenovirus-like particles (ADDomers) as a snakebite therapy
Orientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2021 - Atual Bioprocess intensification for production of cell-based vaccines
Orientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2021 - Atual Bioprocess engineering strategies for improve VLP-based vaccines
Orientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2018 - Atual Bioprocess engineering of insect cells for enhanced production of influenza VLPs
Orientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2024/01/01 - 2027/12/31 Improving ADDomer manufacturability with continuous downstream processing
Coorientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2024/01/01 - 2027/12/31 Improving lentiviral vector bioprocessing using an engineering approach
Coorientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2024/01/01 - 2027/12/31 Bridging genetic engineering and transcriptomics of insect cells to enhance production of biopharmaceuticals
Orientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2021 - 2024 Bioprocess Engineering of Ferritin-based Nanoparticles as SARS-CoV-2 Vaccine Candidates
Orientador
Instituto de Biologia Experimental e Tecnológica, Portugal
2019 - 2023 Bioprocess Engineering of Insect Cells for Accelerating Vaccines Development
Orientador de Barbara Fernandes
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019 - 2023 Improving production of influenza VLP-based vaccines in insect cells using single-cell analysis
Coorientador de Marco Silvano
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019 - 2023 Combining single-cell analysis with systems biology to fine-tune production of rAAV vectors in insect cells
Coorientador de Nikolaus Virgolini
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018 - 2022 Bioprocess engineering of an “universal” influenza VLP-based vaccine
Orientador de Ricardo Correia
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2015 - 2020 Streamlining Upstream Processing of Complex Biopharmaceuticals
Orientador de Marcos Sousa
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2013 - 2020 Engineering stable insect cell expression systems using site-specific integration technology for production of complex proteins
Orientador de Mafalda Dias
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018 - 2018 Water-free liquid systems for enhancing stability of next generation Influenza vaccines
Orientador de Carolina Richheimer
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018 - 2018 Bioprocess engineering of insect cells for enhanced production of influenza VLPs
Orientador de Léa Marchand
École Supérieure de Biologie, Biochimie, Biotechnologies , França
2017 - 2018 Optimizing the production of zika and influenza Virus-Like-Particles in insect cells
Orientador de João Miguel Marques Vindeirinho
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015 - 2015 Upstream process optimization for the production of Influenza virus-like particles
Orientador de Evelien Vaessen
Wageningen Universiteit en Research, Países Baixos
2014 - 2015 Exploring insect cells versatility for production of influenza VLPs
Coorientador de Daniela Filipa Policarpo Sequeira
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação) 		Instituição / Organização
2022 - Atual Member of the Organizing Committee of “27th ESACT” meeting, Portugal (2022)
2024/05/17 - 2024/05/24 Chair of the Poster Prize Jury of “Vaccine Technology IX” meeting, Mexico (2024/05/17 - 2024/05/24)
Conferência
2019 - 2019 Co-chair of the Baculovirus Expression Technology track in ISBioTech (International Society for BioProcess Technology) Meeting (2019-now). (2019 - 2019)
Conferência (Membro da Comissão Científica)
2019 - 2019 Member of the Poster Prize Jury of “26th ESACT” meeting, Denmark (2019) (2019 - 2019)
Conferência (Outra)
2016 - 2016 Chair of the Poster Prize Jury of “Vaccine Technology VI” meeting, Portugal (2016) (2016 - 2016)
Conferência (Outra)
2014 - 2015 Participation in the organization of the “Cells for Compound Screening” course, Spain (2014-2015) (2014 - 2015)
Oficina (workshop) (Membro da Comissão Organizadora)
2011 - 2015 Participation in the organization of the “Animal Cell Technology” course, Spain (2011-2015) (2011 - 2015)
Oficina (workshop) (Membro da Comissão Organizadora)
2008 - 2008 Participation in the organization of “Vaccine Technology II” meeting, Portugal (2008) (2008 - 2008)
Conferência (Membro da Comissão Organizadora)
2004 - 2004 Participation in the organization of “3rd Recombinant Protein Production Meeting” meeting, Portugal (2004) (2004 - 2004)
Conferência (Membro da Comissão Organizadora)

Participação em evento

Descrição da atividade
Tipo de evento 		Nome do evento
Instituição / Organização
2023/09/05 - Atual Grant writing course (6h, Margot Pilon), Oeiras, Portugal
2022 - Atual Leadership and Management Skills for Group Leaders (24h, Hfp Consulting, Sintra, Portugal)
Instituto de Biologia Experimental e Tecnológica, Portugal
2017 - Atual Grant Writing Residential Intensive (40h, Magdalena Bak-Maier), NOVA, Portugal
Instituto de Biologia Experimental e Tecnológica, Portugal
2019 - 2019 Prince2 Foundation in Project Management (21h, Axelos/PeopleCert)
Oficina (workshop)
Instituto de Biologia Experimental e Tecnológica, Portugal
2018 - 2018 Effective Communication Skills (16h, ECC)
Oficina (workshop)
Instituto de Biologia Experimental e Tecnológica, Portugal
2014 - 2014 Time Management Course (8h, Synergie)
Oficina (workshop)
Instituto de Biologia Experimental e Tecnológica, Portugal

Júri de grau académico

Tema
Tipo de participação 		Nome do candidato (Tipo de grau)
Instituição / Organização
2022 Advancing development of a hepatitis C virus vaccine for human use
Arguente principal
 Anna Falden Offersgaard (Doutoramento)
Københavns Universitet Center for Epidemiologi og Screening, Dinamarca
2021 Scale-down technologies for perfusion culture for rapid biopharmaceutical process development
Arguente principal
 Molly Beth Tregidgo (Doutoramento)
University College London, Reino Unido
2019 Bioprocess engineering and characterization of HIV virus-like particle production in insect cells,
Arguente principal
 Eduard Puente Massaguer (Doutoramento)
Universitat Autònoma de Barcelona, Espanha
2019 Production of a VSV-vectored Ebola vaccine candidate with the Vero cell line
Arguente principal
 José Pedro Losa (Mestrado)
Universidade do Porto Faculdade de Engenharia, Portugal
2018 Taylor-made plant cell cultures for the improved production of recombinant proteins
Arguente
 Ana Rita Basilio Santos (Doutoramento)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014 Efeitos do enriquecimento em oxigénio e da fonte de carbono em operação em biorreactor na produção de pDNA numa estirpe de Escherichia coli
Arguente principal
 Maria Alexandra Vieira Marcelino (Mestrado)
Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal

Consultoria / Parecer

Descrição da atividade Instituição / Organização
2023/09/08 - Atual Consultancy for DiaSorin Italia S.p.A. (Italy)
2017/04/04 - Atual Consultancy for Mymetics (The Netherlands)

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
2021 - Atual Invited lecture at the 11th ESACT Animal Cell Technology Course - Integrated bioprocesses for cell culture based vaccines (Outros)
2021 - Atual Invited lecturer in the Tópicos Avançados em Microbiologia 2 module at the Molecular and Genetic Biology Master Programme from FCUL (Portugal) - lecture on Next Generation Vaccines: Biomanufacturing process. (Mestrado) Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal
2020 - Atual Invited lecturer in the Biofarmacos module at the Biochemistry for Health Master Programme from ITQB-NOVA (Portugal) - Lecture on Comparação sistemas de expressão para a produção proteínas recombinantes produtos; vacinas, vlp's, MAbs (Mestrado) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019 - Atual Coordinator and lecturer in the Manufacturing and Quality Process Control module at the Vaccinology and Drug Development Master Program from University of Siena (Italy) – co-coordination with Otfried Kistner (Senior Consultant & Independent Vaccine Expert, Austria) and Sue Ann Clemens (MD, PhD, Head of IFGH; Director, Master Program in Vaccinology and Drug Development, Prof Pediatric Infectious Diseases, Italy). (Master)
2019 - Atual Coordinator and lecturer in the Fundamentals of Bioengineering module of the Molecular Biosciences PhD Program from ITQB-NOVA (Portugal) - lecture on Bioprocessing Engineering Principles, co-coordination with Manuel Carrondo (iBET, Portugal). (Doutoramento) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019 - Atual Invited lecture at 8th International School on Production of Biologicals, Rio de Janeiro, Brazil - Vaccines: DNA, mRNA, recombinant proteins and virus-like particles (Master) Universidade Federal do Rio de Janeiro, Brasil
2017 - Atual Coordinator of the Curricular Core Unit Animal Cell Technology from PhD Program Bioengineering of Cell Therapies and Regenerative Medicine (IST, CEDOC, IMM and iBET; Portugal) co-coordination with Ana Azevedo (IST, Portugal). (Curso de doutoramento (conclusão de unidades curriculares))
2016 - Atual Invited lecturer in the Tecnologia de Células Animais module at the Chemical and Biochemical Engineering Master of FCT-UNL (Portugal) – lecture on Cells for Production: Insect Cells and the baculovirus system, VLPs and case studies and Bioreactors for Animal Cells Bioprocesses: exercises. (Master)
2015 - Atual Invited lecturer in the Frontiers in biotechnology module of the Molecular Biosciences PhD Program from ITQB-NOVA (Portugal) - lecture on Biofuels. (Curso de doutoramento (conclusão de unidades curriculares))

Membro de associação

Nome da associação Tipo de participação
2011 - Atual Member of the European Society for Animal Cell Technology (ESACT)
Distinções

Prémio

2017 Prize for 2nd Poster Presentation, ESACT 2017 Meeting in Lausanne, Switzerland
2014 Investigador FCT, Fundação para a Ciência e a Tecnologia, Portugal
2013 Travel grant (attend BME conference in Beijing, China), ÅForsk, Sweden
2013 Travel grant (attend BME conference in Beijing, China), Knut och Alice Wallenbergs Stiftelse, Sweden
2013 Travel grant (attend PYFF5 conference in Montpellier, France), Bo Rydins Stiftelse, Sweden
2011 Best PhD thesis, ITQB-UNL/IBET, Portugal
2009 Travel grant (attend ICSB 10 in USA), Fundação Calouste Gulbenkian, Portugal
2008 Travel grant (attend ESF 2nd Functional Dynamics Conference, Germany), ESF, Germany
2006 PhD grant, Fundação para a Ciência e Tecnologia, Portugal
2006 Travel grant (attend CCE X conference in Canada), Cell Culture Engineering X
2003 PRODEP III, PRODEP III, Portugal