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Jose Silva Ramalho (JSR) earned his PhD in Molecular Genetics in 2001 from the Imperial College Division of Molecular Biosciences. Currently, Jose serves as the Principal Investigator/senior scientist in the "Proteostasis and Intercellular Communication" group (Group Leader: Paulo Pereira) at Nova Medical School. His pivotal role involves the development and application of molecular and genetic tools to advance the group's research objectives. Additionally, Jose has supervised and co- supervised several PhD and Master's students within this group, contributing significantly to their academic and research development. Jose’s scholarly contributions include authoring over 70 publications, reflecting extensive national and international collaborations that underscore his prominence in the scientific community. Beyond his academic achievements, Jose has demonstrated a keen entrepreneurial spirit. In 2008, production company located in central Portugal. The company has garnered various prizes and awards, attesting to its innovation and impact. The antibodies developed by SICGEN have been instrumental in numerous high-impact research studies, further establishing Jose's commitment to advancing scientific inquiry and developing innovative applications.
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Identificação

Identificação pessoal

Nome completo
Jose Silva Ramalho

Nomes de citação

  • Ramalho, Jose

Identificadores de autor

Ciência ID
FE13-BC0C-D253
ORCID iD
0000-0002-1927-164X

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1)
Inglês Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1)
Francês Utilizador elementar (A1)
Formação
Grau Classificação
2001
Concluído
 PhD-Molecular Genetics (Doktor (PhD))
Imperial College Division of Molecular Biosciences, Reino Unido
"Analysis of mammalian RAB27A and RAB27B genes and implications for disease" (TESE/DISSERTAÇÃO)
 Satisfied
1995
Concluído
 Biologia Celular (Mestrado)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Influência da glicosilação não enzimática na insolubilização e modificação das proteínas do cristalino" (TESE/DISSERTAÇÃO)
 Muito Bom
1990
Concluído
 Bioquímica (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"....." (TESE/DISSERTAÇÃO)
 13
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento 		Empregador
2009/04/13 - Atual Investigador principal (carreira) (Investigação) Universidade Nova de Lisboa Centro de Estudos de Doenças Crónicas, Portugal
2002/01/01 - 2009/04/12 Investigador Auxiliar (carreira) (Investigação) Universidade de Coimbra Faculdade de Medicina, Portugal

Outros

Categoria Profissional
Instituição de acolhimento 		Empregador
1999/12/01 - 2002/01/01 Assistente de Investigação Universidade de Coimbra Faculdade de Medicina, Portugal
1994/09/01 - 1997/09/01 Assistente de Investigação Universidade de Coimbra Centro de Oftalmologia e Ciências da Visão, Portugal
1992/09/01 - 1994/09/01 Bolseiro de Investigação Universidade de Coimbra Centro de Oftalmologia e Ciências da Visão, Portugal
1990/09/01 - 1992/09/01 Bolseiro de Investigação Universidade de Coimbra Centro de Oftalmologia e Ciências da Visão, Portugal
Projetos

Bolsa

Designação Financiadores
2014/04 - 2015/03 Molecular mechanism of melanin transfer in skin pigmentation
133401
Provided by PTCRIS: 133401
 Fundação para a Ciência e a Tecnologia, I.P.
2011/03 - 2015/02 Tumorigenesis as a working model for the physiological role of HIF-1alpha degradation by chaperone-mediated autophagy
113542
Provided by PTCRIS: 113542
 Fundação para a Ciência e a Tecnologia, I.P.
2011/03 - 2014/08 Molecular mechanisms of melanosome transfer and processing by keratinocytes
Provided by PTCRIS: 111735
111735
 Fundação para a Ciência e a Tecnologia, I.P.
2010/02 - 2013/07 Functional genomics studies of phagocytosis in retinal pigment epithelium
Provided by PTCRIS: 105175
105175
 Fundação para a Ciência e a Tecnologia, I.P.
2010/01 - 2013/06 Regulation of retinal growth factors secretion by Rab GTPases
104668
Provided by PTCRIS: 104668
 Fundação para a Ciência e a Tecnologia, I.P.
2008/11 - 2012/03 A new route for endothelial dysfunction on diabetes: From phenotypes to molecules
67498
Provided by PTCRIS: 67498
 Fundação para a Ciência e a Tecnologia, I.P.
2007/06 - 2010/12 Regulation of AMPA receptors by hyperglycemia in the retina
Provided by PTCRIS: 71228
71228
 Fundação para a Ciência e a Tecnologia, I.P.
2007/01 - 2008/11 Filling in the GAP: the missing link between intercellular communication and diabetic retinopathy.
Provided by PTCRIS: 82324
82324
 Fundação para a Ciência e a Tecnologia, I.P.
2007/01 - 2008/10 What HIF? Degradation is better than growth in preventing angiogenesis in diabetic retinopathy
82730
Provided by PTCRIS: 82730
 Fundação para a Ciência e a Tecnologia, I.P.
2005/10 - 2007/12 Filling in the GAP: the missing link between intercellular communication and diabetic retinopathy.
Provided by PTCRIS: 57216
57216
 Fundação para a Ciência e a Tecnologia, I.P.
2005/09 - 2007/12 What HIF? Degradation is better than growth in preventing angiogenesis in diabetic retinopathy
Provided by PTCRIS: 57772
57772
 Fundação para a Ciência e a Tecnologia, I.P.

Projeto

Designação Financiadores
2020/06/01 - 2021/12/31 Desenvolvimento e produção de anticorpos anti-SARS-CoV-2
69378
Investigador responsável
SICGEN – INVESTIGAÇÃO E DESENVOLVIMENTO EM BIOTECNOLOGIA LDA, Portugal
 IAPMEI
Concluído
Produções

Publicações

Artigo em revista
  1. Elizeth Lopes; Gisela Machado-Oliveira; Catarina Guerreiro Simões; Inês S. Ferreira; Cristiano Ramos; José Ramalho; Maria I. L. Soares; et al. "Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts". Cells (2023): https://doi.org/10.3390/cells12242826.
    10.3390/cells12242826
  2. Juliana Gonçalves; Magda Melro; Marta Alenquer; Catarina Araújo; Júlia Castro-Neves; Daniela Amaral-Silva; Filipe Ferreira; et al. "Balance between maternal antiviral response and placental transfer of protection in gestational SARS-CoV-2 infection". JCI Insight (2023): https://doi.org/10.1172/jci.insight.167140.
    10.1172/jci.insight.167140
  3. Luís C. Cabaço; Liliana Bento-Lopes; Matilde V. Neto; Andreia Ferreira; Wanja B.L. Staubli; José S. Ramalho; Miguel C. Seabra; Duarte C. Barral. "RAB3A Regulates Melanin Exocytosis and Transfer Induced by Keratinocyte-Conditioned Medium". JID Innovations (2022): https://doi.org/10.1016/j.xjidi.2022.100139.
    10.1016/j.xjidi.2022.100139
  4. Hugo Moreiras; Liliana Bento-Lopes; Matilde V. Neto; Cristina Escrevente; Luís C. Cabaço; Michael J. Hall; José S. Ramalho; Miguel C. Seabra; Duarte C. Barral. "Melanocore uptake by keratinocytes occurs through phagocytosis and involves protease-activated receptor-2 internalization". Traffic (2022): https://doi.org/10.1111/tra.12843.
    10.1111/tra.12843
  5. João Vasco Ferreira; Ana da Rosa Soares; José Ramalho; Catarina Máximo Carvalho; Maria Helena Cardoso; Petra Pintado; Ana Sofia Carvalho; et al. "LAMP2A regulates the loading of proteins into exosomes". Science Advances 8 12 (2022): https://doi.org/10.1126/sciadv.abm1140.
    10.1126/sciadv.abm1140
  6. Cristina Escrevente; Liliana Bento-Lopes; José S. Ramalho; Duarte C. Barral. "Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB". Journal of Cell Science 134 11 (2021): https://doi.org/10.1242/jcs.246694.
    10.1242/jcs.246694
  7. Catarino, Steve; Ribeiro-Rodrigues, Teresa M; Sá Ferreira, Rita; Ramalho, José; Abert, Christine; Martens, Sascha; Girão, Henrique. "A Conserved LIR Motif in Connexins Mediates Ubiquitin-Independent Binding to LC3/GABARAP Proteins". Cells 9 4 (2020): 902. http://dx.doi.org/10.3390/cells9040902.
    10.3390/cells9040902
  8. Ferreira, Joao Vasco; Rosa Soares, Ana; Ramalho, José S.; Ribeiro-Rodrigues, Teresa; Máximo, Catarina; Zuzarte, Mónica; Girão, Henrique; Pereira, Paulo. "Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis". PLOS ONE 14 10 (2019): e0223790. http://dx.doi.org/10.1371/journal.pone.0223790.
    10.1371/journal.pone.0223790
  9. Casalou, Cristina; Faustino, Alexandra; Silva, Fernanda; Ferreira, Inês C.; Vaqueirinho, Daniela; Ferreira, Andreia; Castanheira, Pedro; et al. "Arl13b Regulates Breast Cancer Cell Migration and Invasion by Controlling Integrin-Mediated Signaling". Cancers 11 10 (2019): 1461. http://dx.doi.org/10.3390/cancers11101461.
    10.3390/cancers11101461
  10. Kuhns, Stefanie; Seixas, Cecília; Pestana, Sara; Tavares, Bárbara; Nogueira, Renata; Jacinto, Raquel; Ramalho, José S; et al. "Rab35 controls cilium length, function and membrane composition". EMBO reports 20 10 (2019): http://dx.doi.org/10.15252/embr.201847625.
    10.15252/embr.201847625
  11. Casal, Diogo; Iria, Inês; Ramalho, José S.; Alves, Sara; Mota-Silva, Eduarda; Mascarenhas-Lemos, Luís; Pontinha, Carlos; et al. "BD-2 and BD-3 increase skin flap survival in a model of ischemia and Pseudomonas aeruginosa infection". Scientific Reports 9 1 (2019): http://dx.doi.org/10.1038/s41598-019-44153-y.
    10.1038/s41598-019-44153-y
  12. Robinson, Christopher L.; Evans, Richard D.; Sivarasa, Kajana; Ramalho, Jose S.; Briggs, Deborah A.; Hume, Alistair N.. "The adaptor protein melanophilin regulates dynamic myosin-Va:cargo interaction and dendrite development in melanocytes". Molecular Biology of the Cell 30 6 (2019): 742-752. http://dx.doi.org/10.1091/mbc.e18-04-0237.
    10.1091/mbc.e18-04-0237
  13. Oriol Bover; Michael Schubert; Tiago Justo; Paulo N. G. Pereira; João Facucho-Oliveira; José M. Inácio; José S. Ramalho; Ibrahim J. Domian; José António Belo. "Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation in mouse embryonic stem cells". PLOS ONE (2018): https://doi.org/10.1371/journal.pone.0205108.
    10.1371/journal.pone.0205108
  14. Calado, Joaquim; Santos, Ana Rita; Aires, Inês; Lebre, Firmina; Nolasco, Fernando; Rueff, José; Ramalho, José. "The Na + -coupled glucose transporter SGLT 2 interacts with its accessory unit MAP 17 in vitro and their expressions overlap in the renal proximal tubule". FEBS Letters 592 19 (2018): 3317-3326. http://dx.doi.org/10.1002/1873-3468.13233.
    10.1002/1873-3468.13233
  15. Carrascal, Mylène A.; Silva, Mariana; Ramalho, José S.; Pen, Cláudia; Martins, Manuela; Pascoal, Carlota; Amaral, Constança; et al. "Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation". Molecular Oncology 12 5 (2018): 579-593. http://dx.doi.org/10.1002/1878-0261.12163.
    10.1002/1878-0261.12163
  16. Correia, Maria S.; Moreiras, Hugo; Pereira, Francisco J.C.; Neto, Matilde V.; Festas, Tiago C.; Tarafder, Abul K.; Ramalho, José S.; Seabra, Miguel C.; Barral, Duarte C.. "Melanin Transferred to Keratinocytes Resides in Nondegradative Endocytic Compartments". Journal of Investigative Dermatology 138 3 (2018): 637-646. http://dx.doi.org/10.1016/j.jid.2017.09.042.
    10.1016/j.jid.2017.09.042
  17. Robinson CL; Evans RD; Briggs DA; Ramalho JS; Hume AN. "Deficiency in kinesin-1 recruitment to melanosomes precludes it from facilitating their centrifugal transport.". (2017): http://europepmc.org/abstract/med/28490438.
    10.1242/jcs.186064
  18. Ramalho, Jose. "Correction: shRNA-Based Screen Identifies Endocytic Recycling Pathway Components That Act as Genetic Modifiers of Alpha-Synuclein Aggregation, Secretion and Toxicity". PLOS Genetics 12 6 (2016): e1006148. http://dx.doi.org/10.1371/journal.pgen.1006148.
    10.1371/journal.pgen.1006148
  19. Marisa Encarnação; Lília Espada; Cristina Escrevente; Denisa Mateus; José Ramalho; Xavier Michelet; Inês Santarino; et al. "A Rab3a-dependent complex essential for lysosome positioning and plasma membrane repair". Journal of Cell Biology 213 6 (2016): 631-640. https://doi.org/10.1083/jcb.201511093.
    10.1083/jcb.201511093
  20. Vale-Costa, S.; Alenquer, M.; Sousa, A.L.; Kellen, B.; Ramalho, J.; Tranfield, E.M.; Amorim, M.J.. "Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors". Journal of Cell Science 129 8 (2016): 1697-1710. http://www.scopus.com/inward/record.url?eid=2-s2.0-84965031549&partnerID=MN8TOARS.
    10.1242/jcs.188409
  21. Gonçalves, S.A.; Macedo, D.; Raquel, H.; Simões, P.D.; Giorgini, F.; Ramalho, J.S.; Barral, D.C.; Ferreira Moita, L.; Outeiro, T.F.. "shRNA-Based Screen Identifies Endocytic Recycling Pathway Components That Act as Genetic Modifiers of Alpha-Synuclein Aggregation, Secretion and Toxicity". PLoS Genetics 12 4 (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-84964900124&partnerID=MN8TOARS.
    10.1371/journal.pgen.1005995
  22. Thieleke-Matos, C.; Lopes da Silva, M.; Cabrita-Santos, L.; Portal, M.D.; Rodrigues, I.P.; Zuzarte-Luis, V.; Ramalho, J.S.; et al. "Host cell autophagy contributes to Plasmodium liver development". Cellular Microbiology 18 3 (2016): 437-450. http://www.scopus.com/inward/record.url?eid=2-s2.0-84958112950&partnerID=MN8TOARS.
    10.1111/cmi.12524
  23. Pereira, P.M.R.; Silva, S.; Ramalho, J.S.; Gomes, C.M.; Girão, H.; Cavaleiro, J.A.S.; Ribeiro, C.A.F.; Tomé, J.P.C.; Fernandes, R.. "The role of galectin-1 in in vitro and in vivo photodynamic therapy with a galactodendritic porphyrin". European Journal of Cancer 68 (2016): 60-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-84990857207&partnerID=MN8TOARS.
    10.1016/j.ejca.2016.08.018
  24. Reichhart N; Markowski M; Ishiyama S; Wagner A; Crespo-Garcia S; Schorb T; Ramalho JS; et al. "Rab27a GTPase modulates L-type Ca(2+) channel function via interaction with the II-III linker of CaV1.3 subunit.". (2015): http://europepmc.org/abstract/med/26235199.
    10.1016/j.cellsig.2015.07.023
  25. Ferreira-Teixeira, M.; Parada, B.; Rodrigues-Santos, P.; Alves, V.; Ramalho, J.S.; Caramelo, F.; Sousa, V.; Reis, F.; Gomes, C.M.. "Functional and molecular characterization of cancer stem-like cells in bladder cancer: A potential signature for muscle-invasive tumors". Oncotarget 6 34 (2015): 36185-36201. http://www.scopus.com/inward/record.url?eid=2-s2.0-84946866394&partnerID=MN8TOARS.
    10.18632/oncotarget.5517
  26. Ferreira JV; Soares AR; Ramalho JS; Pereira P; Girao H. "K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy.". (2015): http://europepmc.org/abstract/med/25958982.
    10.1038/srep10210
  27. Vitorino M; Silva AC; Inácio JM; Ramalho JS; Gur M; Fainsod A; Steinbeisser H; Belo JA. "Xenopus Pkdcc1 and Pkdcc2 Are Two New Tyrosine Kinases Involved in the Regulation of JNK Dependent Wnt/PCP Signaling Pathway.". (2015): http://europepmc.org/abstract/med/26270962.
    10.1371/journal.pone.0135504
  28. Loureiro LR; Carrascal MA; Barbas A; Ramalho JS; Novo C; Delannoy P; Videira PA. "Challenges in Antibody Development against Tn and Sialyl-Tn Antigens.". (2015): http://europepmc.org/abstract/med/26270678.
    10.3390/biom5031783
  29. Chutna O; Gonçalves S; Villar-Piqué A; Guerreiro P; Marijanovic Z; Mendes T; Ramalho J; et al. "The small GTPase Rab11 co-localizes with a-synuclein in intracellular inclusions and modulates its aggregation, secretion and toxicity.". (2014): http://europepmc.org/abstract/med/25092884.
    10.1093/hmg/ddu391
  30. Thieleke-Matos C; da Silva ML; Cabrita-Santos L; Pires CF; Ramalho JS; Ikonomov O; Seixas E; et al. "Host PI(3,5)P2 activity is required for Plasmodium berghei growth during liver stage infection.". (2014): http://europepmc.org/abstract/med/24992508.
    10.1111/tra.12190
  31. Evans RD; Robinson C; Briggs DA; Tooth DJ; Ramalho JS; Cantero M; Montoliu L; et al. "Myosin-Va and dynamic actin oppose microtubules to drive long-range organelle transport.". (2014): http://europepmc.org/abstract/med/25065759.
    10.1016/j.cub.2014.06.019
  32. Casalou C; Seixas C; Portelinha A; Pintado P; Barros M; Ramalho JS; Lopes SS; Barral DC. "Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration.". (2014): http://europepmc.org/abstract/med/24777479.
    10.1242/jcs.143446
  33. Gomes-da-Silva LC; Fernández Y; Abasolo I; Schwartz S Jr; Ramalho JS; Pedroso de Lima MC; Simões S; Moreira JN. "Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform.". (2013): http://europepmc.org/abstract/med/23394132.
    10.2217/nnm.12.174
  34. Gomes-Da-Silva, L.C.; Ramalho, J.S.; Pedroso De Lima, M.C.; Simões, S.; Moreira, J.N.. "Impact of anti-PLK1 siRNA-containing F3-targeted liposomes on the viability of both cancer and endothelial cells". European Journal of Pharmaceutics and Biopharmaceutics 85 3 PART A (2013): 356-364. http://www.scopus.com/inward/record.url?eid=2-s2.0-84888308868&partnerID=MN8TOARS.
    10.1016/j.ejpb.2013.04.007
  35. Tarafder, A.K.; Bolasco, G.; Correia, M.S.; Pereira, F.J.C.; Iannone, L.; Hume, A.N.; Kirkpatrick, N.; et al. "Rab11b Mediates Melanin Transfer between Donor Melanocytes and Acceptor Keratinocytes via Coupled Exo/Endocytosis". Journal of Investigative Dermatology (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84887281343&partnerID=MN8TOARS.
    10.1038/jid.2013.432
  36. Ferreira, J.V.; Fofo, H.; Bejarano, E.; Bento, C.F.; Ramalho, J.S.; Girao, H.; Pereira, P.. "STUB1/CHIP is required for HIF1A degradation by chaperone-mediated autophagy". Autophagy 9 9 (2013): 1349-1366. http://www.scopus.com/inward/record.url?eid=2-s2.0-84884315657&partnerID=MN8TOARS.
    10.4161/auto.25190
  37. Gomes, C.P.; Gomes-da-Silva, L.C.; Ramalho, J.S.; de Lima, M.C.P.; Simões, S.; Moreira, J.N.. "Impact of PLK-1 silencing on endothelial cells and cancer cells of diverse histological origin". Current Gene Therapy 13 3 (2013): 189-201. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878633310&partnerID=MN8TOARS.
    10.2174/1566523211313030004
  38. Barral, D.C.; Garg, S.; Casalou, C.; Watts, G.F.M.; Sandoval, J.L.; Ramalho, J.S.; Hsu, V.W.; Brenner, M.B.. "Arl13b regulates endocytic recycling traffic". Proceedings of the National Academy of Sciences of the United States of America 109 52 (2012): 21354-21359. http://www.scopus.com/inward/record.url?eid=2-s2.0-84871834183&partnerID=MN8TOARS.
    10.1073/pnas.1218272110
  39. Lopes da Silva, M.; Thieleke-Matos, C.; Cabrita-Santos, L.; Ramalho, J.S.; Wavre-Shapton, S.T.; Futter, C.E.; Barral, D.C.; Seabra, M.C.. "The host endocytic pathway is essential for plasmodium berghei late liver stage development". Traffic 13 10 (2012): 1351-1363. http://www.scopus.com/inward/record.url?eid=2-s2.0-84866290594&partnerID=MN8TOARS.
    10.1111/j.1600-0854.2012.01398.x
  40. Gomes-Da-Silva, L.C.; Santos, A.O.; Bimbo, L.M.; Moura, V.; Ramalho, J.S.; Pedroso De Lima, M.C.; Simões, S.; Moreira, J.N.. "Toward a siRNA-containing nanoparticle targeted to breast cancer cells and the tumor microenvironment". International Journal of Pharmaceutics 434 1-2 (2012): 9-19. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864129727&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2012.05.018
  41. Seixas, E.; Ramalho, J.S.; Mota, L.J.; Barral, D.C.; Seabra, M.C.. "Bacteria and protozoa differentially modulate the expression of rab proteins". PLoS ONE 7 7 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84864093386&partnerID=MN8TOARS.
    10.1371/journal.pone.0039858
  42. Barral D; Garg S; Casalou C; Watts G; Ramalho J; Hsu V; Brenner M. "Arl13b regulates early endocytic vesicle trafficking.". (2012): http://europepmc.org/abstract/PMC/PMC3555714.
  43. Tarafder, A.K.; Wasmeier, C.; Figueiredo, A.C.; Booth, A.E.G.; Orihara, A.; Ramalho, J.S.; Hume, A.N.; Seabra, M.C.. "Rab27a targeting to melanosomes requires nucleotide exchange but not effector binding". Traffic 12 8 (2011): 1056-1066. http://www.scopus.com/inward/record.url?eid=2-s2.0-79960175612&partnerID=MN8TOARS.
    10.1111/j.1600-0854.2011.01216.x
  44. Catarino, S.; Ramalho, J.S.; Marques, C.; Pereira, P.; Girão, H.. "Ubiquitin-mediated internalization of connexin43 is independent of the canonical endocytic tyrosine-sorting signal". Biochemical Journal 437 2 (2011): 255-267. http://www.scopus.com/inward/record.url?eid=2-s2.0-79959687376&partnerID=MN8TOARS.
    10.1042/BJ20102059
  45. Mizuno, K.; Ramalho, J.S.; Izumi, T.. "Exophilin8 transiently clusters insulin granules at the actin-rich cell cortex prior to exocytosis". Molecular Biology of the Cell 22 10 (2011): 1716-1726. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955979816&partnerID=MN8TOARS.
    10.1091/mbc.E10-05-0404
  46. Bento, C.F.; Fernandes, R.; Ramalho, J.; Marques, C.; Shang, F.; Taylor, A.; Pereira, P.. "The chaperone-dependent ubiquitin ligase CHIP targets HIF-1a for degradation in the presence of methylglyoxal". PLoS ONE 5 11 (2010): http://www.scopus.com/inward/record.url?eid=2-s2.0-78649753203&partnerID=MN8TOARS.
    10.1371/journal.pone.0015062
  47. Lança, T.; Correia, D.V.; Moita, C.F.; Raquel, H.; Neves-Costa, A.; Ferreira, C.; Ramalho, J.S.; et al. "The MHC class Ib protein ULBP1 is a nonredundant determinant of leukemia/lymphoma susceptibility to ¿d T-cell cytotoxicity". Blood 115 12 (2010): 2407-2411. http://www.scopus.com/inward/record.url?eid=2-s2.0-77950622607&partnerID=MN8TOARS.
    10.1182/blood-2009-08-237123
  48. da Silva LC; Ramalho JS; de Lima MCP; Simões S; Moreira JN. "Targeted gene silencing for cancer treatment.". (2010): http://europepmc.org/abstract/PMC/PMC3255043.
  49. Ramalho, J.S.; Lopes, V.S.; Tarafder, A.K.; Seabra, M.C.; Hume, A.N.. "Myrip uses distinct domains in the cellular activation of myosin VA and myosin VIIA in melanosome transport". Pigment Cell and Melanoma Research 22 4 (2009): 461-473. http://www.scopus.com/inward/record.url?eid=2-s2.0-67749098050&partnerID=MN8TOARS.
    10.1111/j.1755-148X.2009.00567.x
  50. Figueiredo, A.C.; Wasmeier, C.; Tarafder, A.K.; Ramalho, J.S.; Baron, R.A.; Seabra, M.C.. "Rab3GEP is the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes". Journal of Biological Chemistry 283 34 (2008): 23209-23216. http://www.scopus.com/inward/record.url?eid=2-s2.0-53149101728&partnerID=MN8TOARS.
    10.1074/jbc.M804134200
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Outros

Outra produção
  1. Rab3a regulates melanin exocytosis induced by keratinocyte-conditioned medium. 2021. Luís C. Cabaço; Liliana Bento-Lopes; Matilde V. Neto; José S. Ramalho; Miguel C. Seabra; Duarte C. Barral. https://doi.org/10.1101/2021.05.24.445450.
    10.1101/2021.05.24.445450
  2. Melanocore uptake by keratinocytes occurs through phagocytosis and involves Protease-activated receptor-2 activation. 2021. Hugo Moreiras; Matilde V. Neto; Liliana Bento-Lopes; Cristina Escrevente; José S. Ramalho; Miguel C. Seabra; Duarte C. Barral. https://doi.org/10.1101/2021.04.13.439501.
    10.1101/2021.04.13.439501
  3. A novel Rab11-Rab3a cascade required for lysosome exocytosis. 2021. Cristina Escrevente; Liliana Bento-Lopes; José S Ramalho; Duarte C Barral. https://doi.org/10.1101/2021.03.06.434066.
    10.1101/2021.03.06.434066
Atividades

Orientação

Título / Tema
Papel desempenhado 		Curso (Tipo)
Instituição / Organização
2019/06/24 - 2019/08/23 ERASMUS - Recombinant protein production using MBP tag
Orientador
Uniwersytet Gdanski, Polónia
2019/06/17 - 2019/08/16 ERASMUS - Purification and characterization of polyclonal antibodies
Orientador
Uniwersytet Gdanski, Polónia
2019/06/17 - 2019/08/16 ERASMUS - Recombinant protein production using GST tag
Orientador
Uniwersytet Gdanski, Polónia
2019/06/17 - 2019/08/16 ERASMUS - Polyclonal antibody production
Orientador
Uniwersytet Gdanski, Polónia
2018/09/01 - 2019/07/31 ERASMUS - Molecular Biology
Orientador
2018/07/09 - 2018/09/14 ERASMUS - Polyclonal Antibody Production
Orientador
Manisa Celal Bayar Üniversitesi, Turquia
2016/10/01 - 2017/09/30 In vitro and in vivo models to assess cancer metastasis
Coorientador
2016/10/01 - 2017/09/30 Assessment of the effect of nucleolin expression on the phenotype of breast cancer cells
Coorientador
Universidade de Coimbra, Portugal
2017/07/11 - 2017/09/12 ERASMUS - Molecular Biology
Orientador
Uniwersytet Gdanski, Polónia
2017/03/01 - 2017/04/30 Validação de anticorpos policlonais por Western Blot
Orientador
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2017/03/01 - 2017/04/30 Produção de proteínas recombinantes em E. coli para imunização e purificação de anticorpos
Orientador
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2017/03/01 - 2017/04/30 Produção de proteínas recombinantes e caracterização de anticorpos policlonais
Orientador
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2016/03/01 - 2016/04/30 Caracterização de anticorpos policlonais por Western Blot
Orientador
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2016/03/01 - 2016/04/30 Produção de proteínas recombinantes para produção de anticorpos policlonais
Orientador
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2016/03/01 - 2016/03/31 MSc in Cellular and Molecular Biology
Coorientador
Universidade de Coimbra, Portugal
2015/11/16 - 2015/12/11 ERASMUS - Molecular Biology
Orientador
Uniwersytet Gdanski, Polónia
2015/11/16 - 2015/12/11 ERASMUS - Molecular Biology
Orientador
Uniwersytet Gdanski, Polónia
2015/07/01 - 2015/08/31 ERASMUS - Antibody production
Orientador
Uniwersytet Gdanski, Polónia
2015/07/01 - 2015/08/31 ERASMUS - Antibody production
Orientador
Uniwersytet Gdanski, Polónia
2015/03/01 - 2015/04/30 Produção e Caracterização de Anticorpo Policlonal Anti-TUBA4A para uso em Investigação
Orientador
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013/10/01 - 2014/09/30 Optimização de métodos de manipulação da expressão de genes em células primárias e tecidos
Coorientador
Universidade Lusófona, Portugal
2014/06/10 - 2014/09/10 ERASMUS - Antibody production and characterisation
Orientador
Uniwersytet Gdanski, Polónia
2013/06/30 - 2013/09/30 ERASMUS - Antibody production and characterisation
Orientador
Uniwersytet Gdanski, Polónia
2010/10/01 - 2013/09/30 New roles of Rab GTPases on eye diseases: targeting VEGF secretion
Coorientador
Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
2013/06/01 - 2013/08/30 Antibody production
Orientador
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
2012/07/01 - 2012/08/31 Antibody production
Orientador
Universidade de Coimbra, Portugal
2011/05/21 - 2011/08/21 Antibody production
Orientador
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal

Outro júri / avaliação

Descrição da atividade Instituição / Organização
2019/01/25 - 2019/01/25 Júri do Concurso para provimento de um lugar de Investigador auxiliar, na área científica de Ciências da Saúde, do mapa de pessoal do Instituto Politécnico de Leiria Instituto Politécnico de Leiria, Portugal
2015/03/31 - 2015/03/31 Júri do concurso para ocupação de um posto de Investigador Auxiliar, área de Ciências da Visão e Oncobiologia da carreira de Investigação Científica, do mapa de pessoal da Universidade de Coimbra
2010/05/18 - 2010/05/18 Júri do Concurso para provimento de um lugar de Investigador Principal para a área científica de Biologia Celular da carreira de Investigação Científica, do mapa de pessoal da Faculdade de Ciências Médicas da Universidade NOVA de Lisboa Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
Distinções

Prémio

2013 Grant
Bluepharma, Portugal
2012 Grant
Banco Santander Totta, Portugal
1995 Travel
Fundação Calouste Gulbenkian, Portugal
1993 Training