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I obtained my BSc in Biomedical Sciences, in 2021, with a final grade of 15/20. In my last year I produced my first research study entitled: “Estudo do papel do componente dos grânulos de stress CARHSP1 nas doenças de poliglutaminas”. In 2023, I completed my MSc in Biomedical Sciences-disease mechanisms, obtaining a grade of 18/20. Throughout my master’s thesis, I established a novel Cockayne syndrome B neuronal culture model using induced pluripotent stem cells derived from patients, where I obtained a grade of 19/20. This endeavor aimed to draw parallels with the human condition, facilitating the translation of promising outcomes into clinical testing for new therapeutic approaches. In the end of 2023, I transitioned into a role as a research fellow at the Molecular Neuroscience and Gene Therapy (MNGT) Laboratory, under the guidance of Professor Clévio Nóbrega at the Algarve Biomedical Center Research Institute (ABC-RI), University of Algarve. Throughout my research activities I have gained proficiency in a diverse array of techniques, including fibroblast reprogramming into induced pluripotent stem cells, cell culture, Western blot analysis, immunocytochemistry, PCR, immunohistochemistry, analysis of animal slices, and statistical analysis. Furthermore, I have been selected for two poster presentations at international meetings: European Neuroscience Conference by Doctoral Students (ENCONDS) (2023) and Federation of European Neuroscience Societies (FENS) Regional Meeting (FRM) (2023). I was also selected for an oral presentation at 54º Meeting da Sociedade Portuguesa de Farmacologia (SPF) (2024).
Identification

Personal identification

Full name
Cristiana Raquel Rodrigues Madeira

Citation names

  • Cristiana R. Madeira

Author identifiers

Ciência ID
641A-814C-55AF

Knowledge fields

  • Natural sciences - Biological Sciences - Cell Biology
  • Natural sciences - Biological Sciences - Molecular Biology
  • Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism
  • Medical and Health Sciences - Basic Medicine - Human Genetics
  • Medical and Health Sciences - Basic Medicine - Neurosciences
  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2)
Spanish; Castilian Elementary (A2) Intermediate (B1) Elementary (A2) Intermediate (B1)
Education
Degree Classification
2021 - 2023/12/15
Concluded
Ciências Biomédicas- Mecanismos de doenças (Mestrado)
Major in Células estaminais pluripotentes induzidas (iPSCs)
Universidade do Algarve - Campus de Gambelas, Portugal
"Generation of a novel Cockayne syndrome B neuronal culture model from patient-derived induced pluripotent stem cells " (THESIS/DISSERTATION)
18
2018 - 2021/07/01
Concluded
Ciências Biomédicas (Licenciatura)
Universidade do Algarve - Campus de Gambelas, Portugal
"Estudo do papel do componente dos grânulos de stress CARHSP1 nas doenças de poliglutaminas" (THESIS/DISSERTATION)
15
Affiliation

Science

Category
Host institution
Employer
2023/11 - 2024/08 Research Trainee (Research) Universidade do Algarve - Campus de Gambelas, Portugal
Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
Outputs

Publications

Conference abstract
  1. Cristiana R. Madeira; Afonso-Reis, Ricardo; Nóbrega, Clévio David Rodrigues ; Sofia M. Calado; Matos, Carlos A. Corresponding author: Matos, Carlos A. "Generation of a CSB-iPSC disease model as a platform to develop gene therapy approaches for Cockayne Syndrome type B". Paper presented in Meeting of the Portuguese Society of Pharmacology (SPF Meeting), Lisbon, 2024.
    Published
Conference poster
  1. Cristiana R. Madeira; Afonso-Reis, Ricardo; Paulino, Rodrigo; Nóbrega, Clévio David Rodrigues ; Matos, Carlos A; Sofia M. Calado. Corresponding author: Matos, Carlos A. "Neuronal cultures differentiated from patient-derived induced pluripotent stem cells as a novel model of Cockayne syndrome B". Paper presented in European Neuroscience Conference by Doctoral Students (ENCONDS), 2023.
  2. Cristiana R. Madeira; Afonso-Reis, Ricardo; Matos, Carlos A; Sofia M. Calado. Corresponding author: Sofia M. Calado. "Generation of a novel Cockayne syndrome B neuronal culture model from patient-derived induced pluripotent stem cells". Paper presented in FENS Regional Meeting (FRM), 2023.
  3. Paulino, Rodrigo; Costa, Rafael; Cristiana R. Madeira; Nóbrega, Clévio David Rodrigues . Corresponding author: Nóbrega, Clévio David Rodrigues. "Comet assay: optimization and evaluation of DNA damage in neuronal cells". Paper presented in European Neuroscience Conference by Doctoral Students (ENCONDS), 2023.
  4. Paulino, Rodrigo; Afonso-Reis, Ricardo; Cristiana R. Madeira; Nóbrega, Clévio David Rodrigues. "Evaluation of DNA damage on neuronal cells through single cell gel electrophorasis". Paper presented in Ciência, 2023.
Thesis / Dissertation
  1. "Generation of a novel Cockayne syndrome B neuronal culture model from patient-derived induced pluripotent stem cells". Master, Universidade do Algarve Faculdade de Ciências e Tecnologia, 2023.
  2. "Estudo do papel do componente dos grânulos de stress CARHSP1 nas doenças de poliglutaminas". Degree, Universidade do Algarve - Campus de Gambelas, 2021.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/02/08 Generation of a CSB-iPSC disease model as a platform to develop gene therapy approaches for Cockayne Syndrome type B Meeting of the Portuguese Society of Pharmacology (SPF Meeting) 2024
SOF 2024 (Lisbon, Portugal)