Ana Margarida da Cruz Ledo (3B1A-1D1E-097D)

Curriculum Vitae

Ana Margarida da Cruz Ledo

Ana Ledo concluded her degree in Biochemistry at the University of Coimbra in 2000, followed by a Ph.D. in Biochemistry in 2007, also at the University of Coimbra, Portugal. In 2014 she was selected as a Ciencia Researcher and is currently an Assistant Professor at the Faculty of Pharmacy University of Coimbra and a researcher at the Center for Neuroscience and Cell Biology, U. Coimbra, Portugal. Her early research focused on understanding the role of nitric oxide (NO) in the modulation of neurometabolic and neurovascular coupling. Early achievements include the determination of neuronal-derived NO concentration dynamics and its impact on tissue oxygen consumption, confirming the role of NO as a regulator of neurometabolism at the tissue level. These results were published in high-impact journals including PNAS (IF. 11.2, DOI: 10.1073/pnas.0503624102), Mol Asp in Med (IF. 14.2; DOI: 10.1016/j.mam.2004.02.010), and Free Rad Biol Med (IF. 7.4; DOI: 10.1016/j.freeradbiomed.2010.01.024). More recently she has focused on linking changes in NO bioactivity and bioavailability to derailment of neurovascular and neurometabolic in aging and neurodegeneration, published in Exp. Neurol. (DOI: 10.1016/j.expneurol.2017.01.013), Neurobiol. Aging (DOI: 10.1016/j.neurobiolaging.2016.06.012), Cell Mol. Neurobiol. (DOI: 10.1007/s10571-014-0115-0) and Front. Physiol.(DOI: 10.3389/fphys.2018.00913) and understanding the role of high-fat diets in precipitating neurological alterations (J. Alz. Dis. DOI: 10.3233/JAD-210122). Additionally, she investigated the putative role of hydrogen peroxide as a volume transmission signaling molecule in the brain, recently published in Redox Biology (IF. 11.8, DOI: 10.1016/j.redox.2022.102250). In parallel, she has designed novel tools for in vivo monitoring in awake behaving rodent models namely micro(bio)sensors for oxygen, glucose, lactate, glutamate, and novel bioanalytical tools for the study of brain bioenergetic metabolism coupled to neuronal activity in vivo Sens. Act. B (IF. 7.5, DOI: 10.1016/j.snb.2020.127899) and Biosens. Bioelectron. (IF. 10.6; 10.1016/j.bios.2021.113874). She has had 2 grants approved as PI, focused on the role of NO signaling in the pathophysiology of neurodegeneration. She is since 2018 Co-PI of the project "Monitoring neurometabolic markers in vivo using microelectrode-based biosensors". She has co-supervised 1 Ph.D. thesis and 2 MSc Thesis and is currently co-supervisor of one Ph.D. student and one MSc student. Furthermore, she has received 5 awards, the most recent of which is the "Leopold Flohé Redox Pioneer Young Investigator Award" in the 2019 SFRR-Europe Meeting, aimed at distinguishing "scientist < 45 years with outstanding novel findings in the field of biological redox processes, working already independently, and leading an own group of young scientists having published high-quality papers." She has acted as evaluator for two grants (2020 - The Freiburg Institute for Advanced Studies FRIAS COFUND Fellowship Programme (FCFP) and 2016 - France Alzheimer & Maladies Apparentées) and reviewer/referee for relevant journals including Redox Biol., Free Rad. Biol. Med., Neurobiol. Dis., J. Physiol. and Nitric Oxide. To date (December 5th, 2023) her h-factor is 17, counting 797 citations (Scopus).

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Identificação

Identificação pessoal

Nome completo: Ana Margarida da Cruz Ledo

Nomes de citação

Ledo, Ana

Identificadores de autor

Ciência ID: 3B1A-1D1E-097D
ORCID iD: 0000-0002-7737-4241

Endereços de correio eletrónico

analedo@cnc.uc.pt (Profissional)
analedo@ff.uc.pt (Profissional)

Telefones

Telefone

(+351) 239488445 (Profissional)

Moradas

Centro de Neurociências e Biologia Celular, Universidade de Coimbra - Fac. Medicina, Polo 1, Piso 1, Rua Larga, 3005-405, Coimbra, Coimbra, Portugal (Profissional)

Websites

http://www.cnbc.pt/research/areaC1_2.asp?lg=2 (Profissional)
nao se aplica (Pessoal)

Domínios de atuação

Ciências Naturais - Ciências Biológicas - Bioquímica
Ciências Médicas e da Saúde - Medicina Básica - Neurociências
Ciências Médicas e da Saúde - Biotecnologia Médica

Idiomas

Idioma	Conversação	Leitura	Escrita	Compreensão
Inglês (Idioma materno)
Português (Idioma materno)
Espanhol; Castelhano	Utilizador independente (B1)	Utilizador independente (B1)	Utilizador elementar (A1)	Utilizador independente (B1)
Francês	Utilizador elementar (A1)	Utilizador independente (B1)	Utilizador elementar (A1)	Utilizador elementar (A1)

Formação

	Grau	Classificação
2018/11/14 - 2018/11/15 Concluído	CMA Microdialysis Course - In vivo Microdialysis in small rodents" (Outros) CMA - Harvard Apparatus, Suécia
2018/03/24 - 2018/03/30 Concluído	E -COST MitoEAGLE - Short-Term Scientific Mission "Respirometric Protocols for Intact Tissue Preparations - Regulation of mitochondrial respiration by bioactive gases" (Outros) University of Szeged - Institute of Surgical Research, Hungria
2014/03/07 - 2014/03/15 Concluído	“Short Visit” - Center for Microelectrode Technology, University of Kentucky, USA (with Prof. Dr. Greg. A. Gerhardt). (Outros) University of Kentucky - Center for Microelectrode Tecnology, Estados Unidos
2013/06/19 - 2013/06/21 Concluído	Ciência em Diálogo (Outros) CNC.IBILI, Portugal Universidade de Coimbra Centro de Estudos Sociais, Portugal
2011/05/13 - 2011/05/21 Concluído	Second-by-Second Electrochemical Measurements in Biological Systems (Outros) University of Kentucky - Center for Microelectrode Tecnology, Estados Unidos
2010/11/29 - 2010/12/03 Concluído	Image Processing and Analysis in Cell Physiology – Advances Training Workshop (Outros) Universidade de Aveiro Departamento de Biologia, Portugal
2009 Concluído	Laboratory Animal Science - CAT. C (Outros) Centro de Neurociências e Biologia Celular, Portugal
2007/12 - 2008/11 Concluído	Pós-doutoramento (Pós-doutoramento) Universidade do Algarve, Portugal
2007 Concluído	Bioquímica (Doutoramento) Especialização em Toxicologia Molecular Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal "Dinâmica de Concentração do Óxido Nítrico Produzido no Hipocampo de Rato por Activação de Receptores do Glutamato (Concentration Dynamics of Nitric Oxide produced by Activation of Glutamate Receptores in the Hippocampus)" (TESE/DISSERTAÇÃO)	Approved, with honors and destinction
2005/04/27 - 2005/04/29 Concluído	International Course on Toxicology - Mitochondria: Between Life and Death (Outros) CNC.IBILI, Portugal
2003/05/12 - 2003/05/16 Concluído	Polyglutamine Expansion Disease (Outros) CNC.IBILI, Portugal
2000/04/10 - 2000/04/14 Concluído	Organotypic Culture of Hippocampal Slices (Outros) Universidade de Coimbra, Portugal
1995 - 2000 Concluído	Biochemistry (Licenciatura) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal "Desenvolvimento de Microssensores para Medição Electroquímica de Óxido Nítrico" (TESE/DISSERTAÇÃO)	16/20

Percurso profissional

Ciência

	Categoria Profissional Instituição de acolhimento	Empregador
2019/01/01 - 2020/08/31	Investigador Contratado (Investigação)	Universidade de Coimbra, Portugal

2017/05 - 2019/01	Investigador Contratado (Investigação)	Universidade de Coimbra, Portugal

2009/05 - 2014/04	Investigador Auxiliar (carreira) (Investigação)	Centro de Neurociências e Biologia Celular, Portugal

2007/12 - 2008/11	Investigador (Investigação)	Centro Biologia Molecular e Estrutural, Portugal

2001/01 - 2007/11	Investigador (Investigação)	Fundação para a Ciência e a Tecnologia, Portugal

Docência no Ensino Superior

	Categoria Profissional Instituição de acolhimento	Empregador
2020/01/09 - Atual	Professor Auxiliar (Docente Universitário)	Universidade de Coimbra Faculdade de Farmácia, Portugal
	Universidade de Coimbra Faculdade de Farmácia, Portugal

Outros

	Categoria Profissional Instituição de acolhimento	Empregador
2014/07/14 - 2020/12/31	General Manager	BrainSense, Lda., Portugal

Projetos

Bolsa

	Designação	Financiadores
2023/03/01 - Atual	The Neurovascular Coupling as a biomarker and a target for cognitive enhancement in aging and vascular dementia through an innovative dietary nitrate-driven nitric oxide redox cycle: a translational study - Investigador	Fundação para a Ciência e a Tecnologia Em curso

Projeto

	Designação	Financiadores
2018/07 - Atual	Monitoring neurometabolic markers in vivo using microelectrode-based biosensors POCI-01-0145-FEDER-028261 Investigador Faculdade de Farmacia, Universidade de Coimbra, Portugal CNC.IBILI, Portugal	Fundação para a Ciência e a Tecnologia COMPETE2020 Em curso
2018/07 - Atual	Cognitive enhancement in aged brain and vascular dementia in humans through improving neurovascular coupling: a mechanism based approach driven by nitrite and ascorbic acid from diet POCI-01-0145-FEDER-29090 Investigador CNC.IBILI, Portugal Faculdade de Farmacia, Universidade de Coimbra, Portugal	Fundação para a Ciência e a Tecnologia COMPETE2020 Em curso
2021/01/01 - 2025/12/31	Center for Innovative Biomedicine and Biotechnology - Associate Laboratory LA/P/0058/2020 Universidade de Coimbra, Portugal	Fundação para a Ciência e a Tecnologia Em curso
2018/07/26 - 2021/07/25	Melhoria cognitiva no cérebro idoso e demência vascular em humanos através da funcionalização do acoplamento neurovascular: uma estratégia mecanística molecular promovida por nitrito e ácido ascórbico da dieta PTDC/MED-NEU/29099/2017 Universidade de Coimbra, Portugal Centro Hospitalar e Universitário de Coimbra EPE, Portugal Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal	Fundação para a Ciência e a Tecnologia Em curso
2019/01/01 - 2019/12/31	CNC. IBILI UID/NEU/04539/2019 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal Universidade de Coimbra, Portugal Universidade de Coimbra Instituto de Ciências Nucleares Aplicadas à Saúde, Portugal Universidade de Coimbra Instituto de Investigação Clínica e Biomédica de Coimbra, Portugal	Fundação para a Ciência e a Tecnologia Concluído
2017/05 - 2019/01	HealthyAging 2020 CENTRO-01-0145-FEDER-000012 Bolseiro de Pós-Doutoramento Universidade de Coimbra, Portugal CNC.IBILI, Portugal	CENTRO2020 PORTUGAL2020 Fundo Europeu de Desenvolvimento Regional Concluído
2013/07 - 2015/09	Nitric oxide is a master regulator of brain energetic metabolism: resolving aerobic glycolysis PTDC/BBB-BQB/3217/2012 Investigador	Fundação para a Ciência e a Tecnologia COMPETE2020 Concluído
2013/05 - 2015/04	A Hipercolesterolemia como factor de risco na doença de Alzheimer: impacto na biodisponibilidade e bioactividade do NO CAPES/FCT: Bioquimica - 323/13 Investigador responsável Centro de Neurociências e Biologia Celular, Portugal Universidade Federal de Santa Catarina, Brasil	Fundação para a Ciência e a Tecnologia Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Concluído
2012/04 - 2015/03	Nitric Oxide in Alzheimer´s Disease - Signaling Molecule and Mediator of Pathogenesis PTDC/BIA-BCM/116576/2010 Investigador responsável CNC.IBILI, Portugal	Fundação para a Ciência e a Tecnologia COMPETE2020 Concluído
2010/05 - 2013/10	Design of composite chemical sensors and biosensors for real-time and simultaneous monitoring of nitric oxide and oxygen in vivo in the brain. PTDC/SAU-BEB/103228/2008 Investigador CNC.IBILI, Portugal Universidade de Coimbra, Portugal	Fundação para a Ciência e a Tecnologia COMPETE2020 Concluído
2010/05 - 2013/10	Neurovascular coupling between neuronal activity and blood flow in brain mediated by nitric oxide PTDC/SAU-NEU/108992/2008 Investigador CNC.IBILI, Portugal	Fundação para a Ciência e a Tecnologia COMPETE2020
2010/06 - 2013/06	In vivo dynamics of nitric oxide in the brain: regulation of cellular respiration and implications for Alzheimer´s disease and aging PTDC/SAU-NEU/103538/2008 Investigador CNC.IBILI, Portugal	Fundação para a Ciência e a Tecnologia COMPETE2020 Concluído
2011/01/01 - 2012/12/31	Strategic Project - LA 1 - 2011-2012 PEst-C/SAU/LA0001/2011 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluído
2010/04 - 2012/03	Development of a micro-probe for capillary laser Doppler flowmetry in rat brain PTDC/SAU-NEU/101726/2008 Investigador Universidade de Coimbra Departamento de Física, Portugal	Fundação para a Ciência e a Tecnologia COMPETE2020 Concluído
2002/03 - 2006/01	Real-time measurement of nitric oxide in hippocampal brain slices: modulation by tissue redox state with implications for neuronal degeneration POCTI/BCI/42365/2001 Bolseiro de Doutoramento CNC.IBILI, Portugal Universidade de Coimbra Faculdade de Farmácia, Portugal	Fundação para a Ciência e a Tecnologia Concluído

Produções

Publicações

Artigo em conferência	Ledo, Ana. "Monitoring hydrogen peroxide in biological media: electrochemical sensing approaches". Trabalho apresentado em Annual Meeting of the Society-for-Free-Radical-Research- Europe (SFRRE) on Redox Homeostasis - From Signaling to Damage, Ferrara, 2019. Publicado Candida Dias; Cátia F. Lourenço; Rui M. Barbosa; Ana Ledo; João Laranjinha. "Nitrite modulates mitochondrial respiration in brain ischemia/reperfusion". Trabalho apresentado em Annual Meeting of the Society-for-Free-Radical-Research- Europe (SFRRE) on Redox Homeostasis - From Signaling to Damage, 2019. Publicado Ana Ledo; Candida dias; Cátia F. Lourenço; Rui M. Barbosa; João Laranjinha. "Nitric oxide bioactivity in the brain - aging and disease". Trabalho apresentado em Annual Meeting of the Society-for-Free-Radical-Research- Europe (SFRRE) on Redox Homeostasis - From Signaling to Damage, Ferrara, 2019. Publicado Tiago Monteiro; Dias, Cândida; Lourenço, Cátia F.; Ledo, Ana; Laranjinha, João; Barbosa, Rui M.; Almeida, M. Gabriela. "Real-time detection of nitrite in the brain using carbon fiber microelectrodes". Trabalho apresentado em The 3rd International Congress of CiiEM-2018 - Translational Research and Innovation in Human and Health Science, Monte da Caparica, 2018. Publicado • https://doi.org/10.1080/07853890.2018.1427445 Ledo, Ana; Lourenço, Cátia; Nunes, Carla; Santos, Daniela; Laranjinha, João; Barbosa, Rui. "Electrochemical (bio)sensors for high-resolution measurements of hydrogen peroxide in biological media". Trabalho apresentado em 19th Biennial Meeting SFRRi2018 Lisbon, Lisbon, 2018. Publicado • 10.1016/j.freeradbiomed.2018.04.476 Gonçalves, J.S.; Dias, C.; Ferreira, N.; Ledo, A.; Barbosa, R.M.; Laranjinha, J.. "The interplay of nitrite:ascorbate:nitric oxide and its contribution to neurovascular coupling in the hippocampus". Trabalho apresentado em 19th Biennial Meeting SFRRi2018 Lisbon, Lisbon, 2018. 10.1016/j.freeradbiomed.2018.04.469 Lourenço, Cátia F.; Ledo, Ana; Caetano, Miguel; Barbosa, Rui M.; Laranjinha, João. "Age-dependent changes of neurovascular coupling and brain metabolism in Fischer 344 rats". Trabalho apresentado em 19th Biennial Meeting SFRRi2018 Lisbon, Lisbon, 2018. Publicado • 10.1016/j.freeradbiomed.2018.04.145 Dias, Cândida; Lourenço, Cátia F.; Barbosa, Rui M.; Laranjinha, João; Ledo, Ana. "Age-dependent impairment of the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic mouse model of Alzheimer's disease". Trabalho apresentado em 19th Biennial Meeting SFRRi2018 Lisbon, Lisbon, 2018. 10.1016/j.freeradbiomed.2018.04.459 Mancini, Gianni; Dias, Cândida; Lourenço, Cátia; Barbosa, Rui M.; Laranjinha, João; de Bem, Andreza F.; Ledo, Ana. "High-fat and high cholesterol diet induces Alzheimer's like disease in mice". Trabalho apresentado em 19th Biennial Meeting SFRRi2018 Lisbon, Lisbon, 2018. 10.1016/j.freeradbiomed.2018.04.027 Dias, Cândida; Monteiro, Tiago; Lourenço, Cátia F.; Ledo, Ana; Almeida, M. Gabriela; Laranjinha, João; Barbosa, Rui M.. "Probing nitrite and nitric oxide biochemistry in the brain in vivo by a novel sensing approach". Trabalho apresentado em 2017 ISN-ESN Meeting, Paris, 2017. Publicado • https://doi.org/10.1111/jnc.14094 Laranjinha, João; Marques, Cátia; Ledo, Ana; Dias, Cândida; Ferreira, Nuno; Barbosa, Rui. "Regulation of neurovascular coupling in the brain by nitrite". Trabalho apresentado em OCC World Congress and Annual SFRR-E Conference 2017 Metabolic Stress and Redox Regulation, Berlin, 2017. 10.1016/j.freeradbiomed.2017.04.065 Lourenço, Cátia F.; Ledo, Ana; Gerhardt, Greg A.; Laranjinha, João; Barbosa, Rui M.. "Neurometabolic changes during cortical spreading depolarization: high-resolution measurements with a lactate-glucose dual microbiosensor array". Trabalho apresentado em Berlin BRAIN & BRAIN PET 2017 — 28th International Symposium on Cerebral Blood Flow, Metabolism and Function and the 13th International Conference on Quantification of Brain Function with PET, Berlim, 2017. Publicado • https://doi.org/10.1177/0271678X17695978 Lourenço, Cátia F.; Ledo, Ana; Gerhardt, Greg A.; Laranjinha, João; Barbosa, Rui M.. "Oxidase-based microbiosensor real-time monitoring of glucose and neuronal activity in awake-behaving animals". Trabalho apresentado em XXVIIth International Symposium on Cerebral Blood Flow, Metabolism and Function and the XIIth International Conference on Quantification of Brain Function with PET, Vancouver, 2015. Publicado • https://doi.org/10.1177/0271678X16639008 Lourenço, Cátia F.; Ledo, Ana; Barbosa, Rui M.; Laranjinha, João. "Derailment in nitric oxide-depedendent neurovascular and neurometabolic coupling in aging". Trabalho apresentado em XXVIIth International Symposium on Cerebral Blood Flow, Metabolism and Function and the XIIth International Conference on Quantification of Brain Function with PET, Vancouver, 2015. Publicado • https://doi.org/10.1177/0271678X16639008 Ferreira, Nuno; Lourenço, Cátia; Ana, Ledo; Rui, Barbosa; Laranjinha, João. "Nitrite supports neurovascular coupling via redox cycling with ascorbate". Trabalho apresentado em Oxygen Club California World Congress “Oxidants and Antioxidants in Biology, Valencia, 2015. Publicado • 10.1016/j.freeradbiomed.2015.07.070 Dias, Candida; Barbosa, Rui M.; Laranjinha, Joao; Ledo, Ana. "Evaluation of Mitochondrial Function in the CNS of Rodent Models of Alzheimer's Disease - High Resolution Respirometry Applied to Acute Hippocampal Slices". Trabalho apresentado em SFRR-E Meeting 2014, Paris, 2014. Publicado • 10.1016/j.freeradbiomed.2014.10.780 Ledo, Ana; Barbosa, Rui M.; Laranjinha, João. "Interacting Profiles of Oxygen and Nitric Oxide Concentration Dynamics in the Hippocampus". Trabalho apresentado em 16th Annual Meeting SFRBM, San Farncisco, 2009. Publicado • 10.1016/j.niox.2012.04.125 Ledo, Ana; Barbosa, Rui M; Laranjinha, Joao. "Endogenously produced nitric oxide regulates oxygen consumption in rat hippocampal slices". Trabalho apresentado em SFRR-Eurpoe 2007 Meeting, Vilamoura, 2007. Publicado • 10.1080/10715760701615118 Laranjinha, J.; Ledo, A.; Frade, J.; Barbosa, R.. "Monitoring brain nitric oxide formation and respiration". Trabalho apresentado em Free Radicals in Montevideo 2007, 2007. Publicado Laranjinha, J; Ledo, A; Frade, J; Ferreira, NN; Barbosa, RM. "Subregional concentration dynamics of nitric oxide in hippocampal slices evoked by stimulation of glutamate NMDA receptor". Trabalho apresentado em SFRR’s 12th Biennial Meeting, Buenos Aires, 2004. Publicado • 10.1016/j.freeradbiomed.2004.02.048 Ledo, Ana; Frade, João; Ferreira, Nuno Ricardo; Barbosa, Rui M; Laranjinha, Joao. "Mapping of Nitric Oxide Production in Rat Hippocampal Slices – an Electrochemical Approach". Trabalho apresentado em 10th Annual Meeting of the Society for Free Radical Biology and Medicine, Seattle, 2003. Publicado • 10.1016/j.freeradbiomed.2003.09.040 Laranjinha, Joao; Ledo, Ana; Frade, João; Barbosa, Rui M. "Real-time Monitoring of Nitric Oxide in Rat Hippocampal Brain Slices". Trabalho apresentado em XIth Meeting of the Society for Free Radical Research Internacional, Paris, 2002. Publicado • 10.1016/S0891-5849(02)00928-0 Ledo, A; Barbosa, RM; Frade, J; Laranjinha, J. "Nitric oxide measurement in rat hippocampal brain slices using electrochemical techniques". Trabalho apresentado em XIth Meeting of the Society for Free Radical Research Internacional, Paris, 2002. Publicado • 10.1016/S0891-5849(02)00928-0
Artigo em revista	Ledo, A.; Fernandes, E.; Barbosa, RM.. "Real-time Neurochemical Sensing of Hydrogen Peroxide Production in Brain Tissue using Advanced Nanostructured Au Microelectrodes Functionalized with Ruthenium Purple Film". Electrochimica Acta 512 (2025): 145447. https://doi.org/10.1016/j.electacta.2024.145447. Acesso aberto • 10.1016/j.electacta.2024.145447 Ledo, Ana; Laranjinha, João; Barbosa, Rui M.. "A biosensor-based approach for the determination of hydrogen peroxide in biological samples". Free Radical Biology and Medicine 218 (2024): 31. http://dx.doi.org/10.1016/j.freeradbiomed.2024.04.112. 10.1016/j.freeradbiomed.2024.04.112 Dias, Cândida; Lourenço, Cátia F.; Laranjinha, João; Ledo, Ana. "Modulation of oxidative neurometabolism in ischemia/reperfusion by nitrite". Free Radical Biology and Medicine 218 (2024): 48. http://dx.doi.org/10.1016/j.freeradbiomed.2024.04.172. 10.1016/j.freeradbiomed.2024.04.172 Fernandes, Eliana; Ledo, Ana; Gerhardt, Greg A.; Barbosa, Rui M.. Autor correspondente: Barbosa, Rui M.. "Amperometric bio-sensing of lactate and oxygen concurrently with local field potentials during status epilepticus". Talanta 268 (2024): 125302. http://dx.doi.org/10.1016/j.talanta.2023.125302. 10.1016/j.talanta.2023.125302 Ledo, Ana; Baldeiras, Inês; Laranjinha, João; Barbosa, Rui M.. "Determination of Hydrogen Peroxide Concentration in Biological Samples: a Biosensor-Based Approach". Free Radical Biology and Medicine 201 (2023): 41-42. http://dx.doi.org/10.1016/j.freeradbiomed.2023.03.171. 10.1016/j.freeradbiomed.2023.03.171 Dias, Cândida; Fernandes, Eliana; Barbosa, Rui M.; Laranjinha, João; Ledo, Ana. Autor correspondente: Ledo, Ana. "Astrocytic Aerobic Glycolysis Provides Lactate to Support Neuronal Oxidative Metabolism in the Hippocampus". Biofactors (2023): No prelo Dias, C.; Lourenço, C.F.; Laranjinha, J.; Ledo, A.. "Modulation of oxidative neurometabolism in ischemia/reperfusion by nitrite". Free Radical Biology and Medicine 193 (2022): 779-786. http://dx.doi.org/10.1016/j.freeradbiomed.2022.11.021. 10.1016/j.freeradbiomed.2022.11.021 Dias, Cândida; Fernandes, Eliana; Barbosa, Rui M.; Ledo, Ana. "A Platinized Carbon Fiber Microelectrode-Based Oxidase Biosensor for Amperometric Monitoring of Lactate in Brain Slices". Sensors 22 18 (2022): 7011. http://dx.doi.org/10.3390/s22187011. 10.3390/s22187011 Regiart, Matias; Ledo, Ana; Fernandes, Eliana; Messina, German A.; Brett, Christopher M.A.; Bertotti, Mauro; Barbosa, Rui M.. "Highly sensitive and selective nanostructured microbiosensors for glucose and lactate simultaneous measurements in blood serum and in vivo in brain tissue". Biosensors and Bioelectronics 199 (2022): 113874. http://dx.doi.org/10.1016/j.bios.2021.113874. 10.1016/j.bios.2021.113874 Fernandes, Eliana; Ledo, Ana; Barbosa, Rui M.. "Design and Evaluation of a Lactate Microbiosensor: Toward Multianalyte Monitoring of Neurometabolic Markers In Vivo in the Brain". Molecules 27 2 (2022): 514. http://dx.doi.org/10.3390/molecules27020514. 10.3390/molecules27020514 Ledo, A.; Fernandes, E.; Salvador, A.; Laranjinha, J.; Barbosa, R.M.. "In vivo hydrogen peroxide diffusivity in brain tissue supports volume signaling activity". Redox Biology (2022): 102250. http://dx.doi.org/10.1016/j.redox.2022.102250. Acesso aberto • No prelo • 10.1016/j.redox.2022.102250 Monteiro, Tiago; Dias, Cândida; Lourenço, Cátia F.; Ledo, Ana; Barbosa, Rui M.; Almeida, M. Gabriela. "Microelectrode Sensor for Real-Time Measurements of Nitrite in the Living Brain, in the Presence of Ascorbate". Biosensors 11 8 (2021): 277. http://dx.doi.org/10.3390/bios11080277. 10.3390/bios11080277 Mancini, Gianni; Dias, Candida; Lourenço, Catia F.; Laranjinha, Joao; de Bem, Andreza; Ledo, Ana. "A High Fat/Cholesterol Diet Recapitulates Some Alzheimer's Disease-Like Features in Mice: Focus on Hippocampal Mitochondrial Dysfunction". Journal of Alzheimer's Disease (2021): 1-15. http://dx.doi.org/10.3233/jad-210122. 10.3233/jad-210122 Ledo, Ana; Fernandes, Eliana; Laranjinha, João; Barbosa, Rui M.. "Diffusion and Removal Kinetics of Hydrogen Peroxide in Brain Tissue". Free Radical Biology and Medicine 165 (2021): 28. http://dx.doi.org/10.1016/j.freeradbiomed.2020.12.326. Publicado • 10.1016/j.freeradbiomed.2020.12.326 Laranjinha, João; Nunes, Carla; Ledo, Ana; Lourenço, Cátia; Rocha, Bárbara; Barbosa, Rui M.. "The Peculiar Facets of Nitric Oxide as a Cellular Messenger: From Disease-Associated Signaling to the Regulation of Brain Bioenergetics and Neurovascular Coupling". Neurochemical Research 46 1 (2021): 64-76. http://dx.doi.org/10.1007/s11064-020-03015-0. Publicado • 10.1007/s11064-020-03015-0 Ledo, A.; Lourenço, C.F.; Cadenas, E.; Barbosa, R.M.; Laranjinha, J.. "The bioactivity of neuronal-derived nitric oxide in aging and neurodegeneration: Switching signaling to degeneration". Free Radical Biology and Medicine 162 (2021): 500-513. http://dx.doi.org/10.1016/j.freeradbiomed.2020.11.005. 10.1016/j.freeradbiomed.2020.11.005 Rodrigues, Matilde S.; Ferreira, Cláudia; Dias, Cândida; Pliássova, Anna; Souza, Lisiane; Ledo, Ana; Laranjinha, João; Cunha, Rodrigo A.; Köfalvi, Attila. "An optimized spectrophotometric assay reveals increased activity of enzymes involved in 2-arachidonoyl glycerol turnover in the cerebral cortex of a rat model of Alzheimer’s disease". European Journal of Neuroscience 55 4 (2020): 1051-1062. http://dx.doi.org/10.1111/ejn.14944. 10.1111/ejn.14944 Ledo, Ana; Fernandes, Eliana; Quintero, Jorge E.; Gerhardt, Greg A.; Barbosa, Rui M.. "Electrochemical Evaluation of a Multi-Site Clinical Depth Recording Electrode for Monitoring Cerebral Tissue Oxygen". Micromachines 11 7 (2020): 632. http://dx.doi.org/10.3390/mi11070632. 10.3390/mi11070632 Ledo, Ana; Fernandes, Eliana; Brett, Christopher M.A.; Barbosa, Rui M.; Brett, Christopher. "Enhanced Selectivity and Stability of Ruthenium Purple-Modified Carbon Fiber Microelectrodes for Detection of Hydrogen Peroxide in Brain Tissue". Sensors and Actuators B: Chemical (2020): 127899. http://dx.doi.org/10.1016/j.snb.2020.127899. Publicado • 10.1016/j.snb.2020.127899 Lourenço, Cátia F.; Caetano, Miguel; Ledo, Ana; Barbosa, Rui M.. "Platinized carbon fiber-based glucose microbiosensor designed for metabolic studies in brain slices". Bioelectrochemistry 130 (2019): 107325. http://dx.doi.org/10.1016/j.bioelechem.2019.06.010. 10.1016/j.bioelechem.2019.06.010 Ana Ledo; Cátia F. Lourenço; João Laranjinha; Greg A. Gerhardt; Rui M. Barbosa. "Concurrent measurements of neurochemical and electrophysiological activity with microelectrode arrays: New perspectives for constant potential amperometry". Current Opinion in Electrochemistry 12 (2018): 129-140. https://www.sciencedirect.com/science/article/pii/S2451910318300073?via%3Dihub. 10.1016/j.coelec.2018.05.018Get Lourenço, Cátia F.; Ledo, Ana; Caetano, Miguel; Barbosa, Rui M.; Laranjinha, João. "Age-Dependent Impairment of Neurovascular and Neurometabolic Coupling in the Hippocampus". Frontiers in Physiology 9 (2018): 913. http://dx.doi.org/10.3389/fphys.2018.00913. Acesso aberto • Publicado • 10.3389/fphys.2018.00913 Dias, Cândida; Lourenço, Cátia F.; Barbosa, Rui M.; Laranjinha, João; Ledo, Ana. "Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices". Analytical Biochemistry 551 (2018): 43-50. http://dx.doi.org/10.1016/j.ab.2018.05.010. Publicado • 10.1016/j.ab.2018.05.010 Ferreira, N.R.; Ledo, A.; Laranjinha, J.; Gerhardt, G.A.; Barbosa, R.M.. "Simultaneous measurements of ascorbate and glutamate in vivo in the rat brain using carbon fiber nanocomposite sensors and microbiosensor arrays". Bioelectrochemistry 121 (2018): 142-150. http://www.scopus.com/inward/record.url?eid=2-s2.0-85041463844&partnerID=MN8TOARS. Publicado • 10.1016/j.bioelechem.2018.01.009 Monteiro, Tiago; Dias, Cândida; Lourenço, Cátia F; Ledo, Ana; Laranjinha, João; Barbosa, Rui M.; Almeida, M. Gabriela. "Real-time detection of nitrite in the brain using carbon fiber microelectrodes". Annals of Medicine 50 S1 (2018): S29-S30. Publicado Ledo, A.; Lourenço, C.F.; Laranjinha, J.; Gerhardt, G.A.; Barbosa, R.M.. "Combined in vivo amperometric oximetry and electrophysiology in a single sensor: A tool for epilepsy research". Analytical Chemistry 89 21 (2017): 12383-12390. http://www.scopus.com/inward/record.url?eid=2-s2.0-85034812431&partnerID=MN8TOARS. Publicado • 10.1021/acs.analchem.7b03452 Ledo, A.; Lourenço, C.F.; Laranjinha, J.; Brett, C.M.A.; Gerhardt, G.A.; Barbosa, R.M.. "Ceramic-Based Multisite Platinum Microelectrode Arrays: Morphological Characteristics and Electrochemical Performance for Extracellular Oxygen Measurements in Brain Tissue". Analytical Chemistry 89 3 (2017): 1674-1683. http://www.scopus.com/inward/record.url?eid=2-s2.0-85026352061&partnerID=MN8TOARS. Publicado • 10.1021/acs.analchem.6b03772 Lourenço, C.F.; Ledo, A.; Barbosa, R.M.; Laranjinha, J.. "Neurovascular uncoupling in the triple transgenic model of Alzheimer's disease: Impaired cerebral blood flow response to neuronal-derived nitric oxide signaling". Experimental Neurology 291 (2017): 36-43. http://www.scopus.com/inward/record.url?eid=2-s2.0-85011654048&partnerID=MN8TOARS. Publicado • 10.1016/j.expneurol.2017.01.013 Lourenço, C.F.; Ledo, A.; Barbosa, R.M.; Laranjinha, J.. "Neurovascular-neuroenergetic coupling axis in the brain: master regulation by nitric oxide and consequences in aging and neurodegeneration". Free Radical Biology and Medicine 108 (2017): 668-682. http://www.scopus.com/inward/record.url?eid=2-s2.0-85018262522&partnerID=MN8TOARS. Publicado • 10.1016/j.freeradbiomed.2017.04.026 Lourenço, C.F.; Ledo, A.; Gerhardt, G.A.; Laranjinha, J.; Barbosa, R.M.. "Neurometabolic and electrophysiological changes during cortical spreading depolarization: Multimodal approach based on a lactate-glucose dual microbiosensor arrays". Scientific Reports 7 1 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85026399048&partnerID=MN8TOARS. Acesso aberto • Publicado • 10.1038/s41598-017-07119-6 Lourenço, Cátia F; Ledo, Ana; Laranjinha, João; Gerhardt, Greg A.; Barbosa, Rui M. "Microelectrode array biosensor for high-resolution measurements of extracellular glucose in the brain". Sensors and Actuators B: Chemical 237 1 (2016): 298-307. Publicado • 10.1016/j.snb.2016.06.083 Dias, Cândida; Lourenço, Cátia F; Ferreiro, Elisabete; Barbosa, Rui M; Laranjinha, João; Ledo, Ana. "Age-dependent changes in the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic model of Alzheimer's disease: implications for neurometabolic regulation". Neurobiology of Aging 46 1 (2016): 84-95. Publicado • 10.1016/j.neurobiolaging.2016.06.012 Lourenço, Cátia F.; Ledo, Ana; Dias, Cândida; Barbosa, Rui M; Laranjinha, João. "Neurovascular and neurometabolic derailment in aging and Alzheimer's disease". Frontiers in Aging Neuroscience 7 103 (2015): 1-8. Publicado • 10.3389/fnagi.2015.00103 Ledo, Ana; Lourenço, Cátia F; Caetano, Miguel; Barbosa, Rui M; Laranjinha, João. "Age-Associated Changes of Nitric Oxide Concentration Dynamics in the Central Nervous System of Fisher 344 Rats". Cellular and Molecular Neurobiology 35 1 (2014): 33-44. Publicado • 10.1007/s10571-014-0115-0 Santos, Ricardo M.; Lourenço, Cátia F.; Ledo, Ana; Barbosa, Rui M.; Laranjinha, João. "Nitric Oxide Inactivation Mechanisms in the Brain: Role in Bioenergetics and Neurodegeneration". International Journal of Cell Biology 2012 na (2012): 1-13. Publicado • 10.1155/2012/391914 Laranjinha, João; Santos, Ricardo M.; Lourenço, Cátia F.; Ledo, Ana; Barbosa, Rui M.. "Nitric oxide signaling in the brain: translation of dynamics into respiration control and neurovascular coupling". Annals of the New York Academy of Sciences 1259 1 (2012): 10-18. Publicado • 10.1111/j.1749-6632.2012.06582.x Barbosa, Rui M; Jesus, António J.; Santos, Ricardo; Pereira, Cassilda; Lourenço, Cátia F; Rocha, Bárbara; Ferreira, Nuno Ricardo; Ledo, Ana; Laranjinha, Joao. "Preparation, Standardization and Measurement of Nitric Oxide Solutions". Global Journal of Analytical Chemsitry - (2011): 1-26. Publicado Ledo, Ana; Barbosa, Rui M; Cadenas, Enrique; Laranjinha, Joao. "Dynamic and Interacting profiles of ·NO and O2 in rat hippocampal slices". Free Radical Biology and Medicine 48 8 (2010): 1044-1050. http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T38-4Y7P6TR-4&_user=2656661&_coverDate=04%2F15%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000057391&_version=1&_urlVersion=0&_userid=2656661&md5=2c67ecc3f4fd88742b1810. Publicado • 10.1016/j.freeradbiomed.2010.01.024 Laranjinha, Joao; Ledo, Ana. "Coordination of physiologic and toxic pathways in hippocampus by nitric oxide and mitochondria.". Frontiers in Bioscience 12 1 (2007): 1094-1106. http://www.bioscience.org/2007/v12/af/2129/fulltext.htm. Publicado • 10.2741/2129 Ledo, Ana; Frade, João; Barbosa, Rui M; Laranjinha, Joao. "Regulation in Nitric Oxide Dynamics in Hippocampus by NMDA and AMPA Glutamate Receptors". Current Topics in Pharmacology 10 2 (2006): 55-70. Publicado Ledo, Ana; Barbosa, Rui M; Gerhardt, G.; Cadenas, Enrique; Laranjinha, Joao. "Concentration dynamics of nitric oxide in rat hippocampal subregions evoked by stimulation of the NMDA glutamate receptor.". Proceedings of the National Academy of Sciences of the United States of America 102 48 (2005): 17483-17488. http://www.pnas.org/content/102/48/17483.long. Publicado • 10.1073/pnas.0503624102 Ferreira, N.R.; Ledo, A.; Frade, J.G.; Gerhardt, G.A.; Laranjinha, J.; Barbosa, R.M.. "Electrochemical measurement of endogenously produced nitric oxide in brain slices using Nafion/o-phenylenediamine modified carbon fiber microelectrodes". Analytica Chimica Acta 535 1-2 (2005): 1-7. Publicado • 10.1016/j.aca.2004.12.017 Ledo, Ana; Frade, João; Barbosa, Rui M; Laranjinha, Joao. "Nitric oxide in brain: diffusion, targets and concentration dynamics in hippocampal subregions.". Molecular Aspects in Medicine 25 1-2 (2004): 75-89. http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T9P-4BY3ND9-2&_user=2656661&_coverDate=04%2F30%2F2004&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=2656661&md5=4c0c44937c20af5c5eb3ae. Publicado • 10.1016/j.mam.2004.02.010 Ledo, Ana; Barbosa, Rui M.; Frade, João; Laranjinha, João. "Nitric oxide monitoring in hippocampal brain slices using electrochemical methods". Methods in Enzymology 359 (2002): 111-125. Publicado
Capítulo de livro	Ledo, Ana; Rocha, Bárbara S.; Laranjinha, João. "Bioactive Lipids and the gut-brain axis: diet as a modulator of bioactivity and diversity of lipids in the brain". In Bioactive Foods in Promoting Health, editado por Homero Rubbo. Springer, 2019. Ledo, Ana; Barbosa, R. M.; Laranjinha, J.. "Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices". In Methods in Molecular Biology, editado por Carlos Palmeira; Antonio Moreno, 89-107. Humana Press, 2018. Publicado • 10.1007/978-1-61779-382-0_5 Lourenço, Cátia F; Ledo, Ana; Barbosa, Rui M; Laranjinha, João. "Nitric oxide in neurovascular coupling: underlying mechanisms and dysfunction in aging and Alzheimer’s disease". In Biochemistry of oxidative stress. Physiopathology and clinical aspects, editado por Juan J Poderoso; Alberto Boveris, 1-10. New York: Spinger, 2016. Publicado Frade, João; Fernandez, C.; Ledo, Ana; Barbosa, Rui M; Laranjinha, João. "The Modulation Of Glutamate NMDA Receptors Activity By Ethanol And Polyphenols” in “In Vitro Systems & the Toxic Effect of Chemicals". In A Toxicological/Pharmacological Approach to Chemico-Biological Interactions at a Membrane Level, editado por Amália Jurado; Maria Conceição Pedroso Lima; Leonor Almeida, 1-10. EUA: Research Signpost, 2012. Publicado Ledo, Ana; Barbosa, Rui M; Laranjinha, João. "Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices". In Mitochondrial Bioenergetics, 73-88. Totowa, NJ: Humana Press, 2012. Publicado • 10.1007/978-1-61779-382-0_5 Frade, João; Ledo, Ana; Nunes, Carla; Barbosa, Rui M; Laranjinha, Joao. "17. Nitric oxide in neurodegenerative pathways.". In Free Radical Pathophysiology, editado por Silvia Alvarez; Pablo Evelson, 309-327. Kerala, Índia: Transworld Research Network, 2008. Publicado
Poster em conferência	Agostinho, Paula; Dias, Cândida; Carvalho, RA; Carvalho, R; Carvalho Rui; Ledo, Ana; Laranjinha, João; Madeira, Daniela; Liliana Dias; et al. Autor correspondente: Attila Köfalvi. "Astrocytic GPR55 Receptors Fuel Glycolysis". Trabalho apresentado em XVII Meeting of the Portuguese Society for Neuroscience, 2021. Fernandes, Eliana; Ledo, Ana; Barbosa, Rui M.. "Development of a microelectrode array biosensor for monitoring lactate dynamics in the brain". Trabalho apresentado em Analítica2020, 2020. Matilde S. Rodrigues; Dias, Cândida; Cláudia Ferreira; Pliássova, Anna; Souza, Lisiane; Ledo, Ana; Laranjinha, João; Cunha, Rodrigo A.; Attila Köfalvi. Autor correspondente: Attila Köfalvi. "Lecture: An optimized spectrophotometric assay reveals increased 2-arachidonoyl glycerol turnover in the cerebral cortex of a rat model of Alzheimer¿s disease". Trabalho apresentado em Meeting of the Portuguese Pharmacological Society, 2020. Ledo, Ana; Lourenço, Cátia F.; Laranjinha, João; Barbosa, Rui M.. ""Aberrant Changes in Oxygen Levels in the Brain during Seizures in Awake Freely Behaving Rodents". Trabalho apresentado em Europhysiology 2018, 2018. Ledo, Ana; Lourenço, Cátia F.; Laranjinha, João; Gerhardt, Greg A.; Barbosa, Rui M.. "Simultaneous recording of neurometabolic changes and local field potential in the central nervous system of awake-behaving rodent models of epilepsy". Trabalho apresentado em 11th FENS Forum of Neuroscience, 2018. Ledo, Ana; Lourenço, Cátia F.; Nunes, Carla; Santos, Daniela; Laranjinha, João; Barbosa, Rui M.. "Electrochemical (bio)sensors for high-resolution measurements of hydrogen peroxide in biological media". Trabalho apresentado em 19th Biennial Meeting SFRRi2018 Lisbon, 2018. Gonçalves, J.S.; Dias, Cândida; Ferreira, N.R.; Ledo, Ana; Barbosa, Rui M.; Laranjinha, João. "The interplay of nitrite:ascorbate:nitric oxide and its contribution to neurovascular coupling in the hippocampus". Trabalho apresentado em 19th Biennial Meeting SFRRi2018 Lisbon, 2018. Dias, Cândida; Lourenço, Cátia F.; Barbosa, Rui M.; Laranjinha, João; Ledo, Ana. "Age-dependent impairment of the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic mouse model of Alzheimer's disease". Trabalho apresentado em 19th Biennial Meeting SFRRi2018 Lisbon, 2018. Barbosa, Rui M.; Lourenço, Cátia F.; Gerhardt, Greg A.; Laranjinha, João; Ledo, Ana. "Monitoring Neurometabolic Markers in Vivo with Electrochemical Microsensor Arrays". Trabalho apresentado em 68th Annual Meeting of the International Society of Electrochemistry, Providence, EUA, 2017. Dias, Cândida; Monteiro, Tiago; Lourenço, Cátia F.; Ledo, Ana; Almeida, M. Gabriela; Laranjinha, João; Barbosa, Rui M.. "“Probing Nitrite and Nitric Oxide Biochemistry in The Brain in vivo by a Novel Sensing Approach". Trabalho apresentado em 2017 ISN-ESN Meeting, Paris, França, 2017. Monteiro, Tiago; Dias, Cândida; Lourenço, Cátia F.; Ledo, Ana; Barbosa, Rui M.; Laranjinha, João. "Micro(bio)sensing tools for nitrite detection in the brain". Trabalho apresentado em XXIV International Symposium on Bioelectrochemistry and Bioenergetics of the Bioelectrochemical Society, Lion, França, 2017. Dias, Cândida; Lourenço, Cátia F.; Barbosa, Rui M.; Laranjinha, João; Ledo, Ana. "Alterations in the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic model of Alzheimer’s disease are age-dependent and affect neurometabolic regulation". Trabalho apresentado em XIX National Congress of Biochemistry (SPB2016), Guimarães, Portugal,, 2016. Lourenço, Cátia F.; Ledo, Ana; Gerhardt, Greg A.; Laranjinha, João; Barbosa, Rui M.. "Neurometabolic changes during cortical spreading depolarization: high-resolution measurements with a lactate-glucose dual microbiosensor array". Trabalho apresentado em XIX National Congress of Biochemistry (SPB2016), Guimarães, Portugal, 2016. Ledo, Ana; Lourenço, Cátia F.; Laranjinha, João; Huettl, Peter; Pomerleau, Francois; Barbosa, Rui M.; Gerhardt, Greg A.. "Simultaneous recording of neurometabolic changes and local eld poten al related currents in rodent models of Epilepsy.”". Trabalho apresentado em Neuroscience 2016, Society for Neuroscience, Chicago, USA, 2016. Ledo, Ana; Lourenço, Cátia F.; Huettl, Peter; Hampson, Robert E. ; Deadwyler, SA.; Laranjinha, João; Barbosa, Rui M.; Gerhardt, Greg A.. "Optimising biomorphic microelectrode arrays for electrophysiological and neurochemical recordings". Trabalho apresentado em Neuroscience 2016, Society for Neuroscience, Chicago, USA, 2016. Ledo, Ana; Lourenço, Cátia F; Laranjinha, João; Gerhardt, Greg A.; Barbosa, Rui M. "Monitoring Neurometabolic Changes in the Central Nervous System of Awake-Behaving Rodent Models of Epilepsy". Trabalho apresentado em 16th Conference of the International Society for Monitoring Molecules in Neuroscience, 2016. Barbosa, Rui M.; Ledo, Ana; Lourenço, Cátia F.; Ferreira, N.R.; Gerhardt, Greg A.; Laranjinha, João; Barbosa, Rui M; Lourenço, Cátia F; Ferreira, Nuno Ricardo. "Microelectrode-based sensors and biosensors for high resolution measurements of neurochemicals in the brain". Trabalho apresentado em Oxygen Club California World Congress “Oxidants and Antioxidants in Biology”, Valência, Espanha, 2015. Ledo, Ana; Lourenço, Cátia Filipa; Gerhardt, G.; Laranjinha, Joao; Barbosa, Rui M. "Neurometabolic Coupling: from Glutamate Neurotransmission to Oxygen and Glucose. Enzyme-Modified Microelectrodes for Monitoring Neurochemicals In Vivo". Trabalho apresentado em IBRO 2015, 2015. Lourenço, Cátia F.; Ledo, Ana; Barbosa, Rui M.; Laranjinha, João. "Derailment in nitric oxide-dependent neurovascular and neurometabolic coupling in aging.". Trabalho apresentado em XXVII Symposium on Cerebral Blood Flow, Metabolism and Function, 2015. Lourenço, Cátia F; Ledo, Ana; Gerhardt, Greg A.; Laranjinha, Joao; Barbosa, Rui M. "Oxidase-based microbiosensor for real time monitoring of glucose and neuronal activity in awake behaving animals". Trabalho apresentado em XXVII International Symposium on Cerebral Blood Flow, Metabolism and Function., 2015. Dias, Cândida; Lourenço, Cátia F; Barbosa, Rui M; Laranjinha, João; Ledo, Ana. "Age-dependent changes in the NMDAr-NO pathway in the hippocampus of a triple transgenic mouse model of Alzheimer’s disease: implications for neurometabolic regulation". Trabalho apresentado em Oxidants and antioxidants in Biology, 2015. Ledo, Ana; Lourenço, Cátia F; Gerhardt, Greg A.; Laranjinha, João; Barbosa, Rui M. "Neurometabolic changes in the central nervous system of awake-behaving rodent models of epilepsy.". Trabalho apresentado em Oxidants and antioxidants in Biology, 2015. Ledo, Ana; Lourenço, Cátia F.; Caetano, Miguel; Barbosa, Rui M.; Laranjinha, João. "In vivo Nitric Oxide Concentration Dynamics in the Central Nervous System of Aging Fischer 344 rats". Trabalho apresentado em XVIII Congress of the Portuguese Society for Biochemistry, Coimbra, Portugal, 2014. Dias, Cândida; Barbosa, Rui M; Laranjinha, Joao; Ledo, Ana. "Evaluation of Mitochondrial Function in the CNS of Rodent Models of Alzheimer's Disease - High Resolution Respirometry Applied to Acute Hippocampal Slices". Trabalho apresentado em SFRR-E Meeting 2014, Cordeliers Campus, Paris, France, 2014. 10.1016/j.freeradbiomed.2014.10.780 Dias, Cândida; Ledo, Ana. "Analysis of the change in mitochondrial OxPhos in intact hippocampal slices from a model of Alzheimer’s disease". Trabalho apresentado em Physiology 2014, London, U.K, 2014. Ledo, Ana; Lourenço, Cátia Filipa; Caetano, Miguel; Barbosa, Rui M; Laranjinha, Joao. "The glutamate-nNOS-nitric oxide pathway is impaired in aging. An in vivo study un Fischer 344 rats". Trabalho apresentado em XVIII Congress of the Portuguese Biochemical Society, 2014. Dias, Cândida; Laranjinha, Joao; Ledo, Ana. "Assessing changes in mitochondrial function in the hippocampus of two rodent models for Alzheimer’s disease using high-resolution respirometry with intact hippocampal slices.". Trabalho apresentado em XVIII Congress of the Portuguese Biochemical Society, 2014. Dias, Cândida; Barbosa, Rui M; Laranjinha, Joao; Ledo, Ana. "Changing Synaptic and Bioenergetic Functions in the Hippocampus in Alzheimer’s Disease - Nitric Oxide Concentration Dynamics in Crisis.". Trabalho apresentado em XVIII Congress of the Portuguese Biochemical Society, 2014. Ledo, Ana; Barbosa, Rui M; Laranjinha, Joao. "Oxygen and Nitric Oxide Concentration Profiles Linked to NMDA Receptor Activity in the Hippocampus of a Model of Alzheimer's Disease". Trabalho apresentado em Gordon Research Conference on Oxidative Stress and Disease, 2013. Ledo, Ana; Barbosa, Rui M; Laranjinha, Joao. "ENDOGENOUS NITRIC OXIDE GOVERNS OXYGEN CONSUMPTION IN THE RAT HIPPOCAMPUS". Trabalho apresentado em INTERNATIONAL SYMPOSIUM ON REDOX SIGNALING AND OXIDATIVE STRESS IN HEALTH AND DISEASE, 2012. Ledo, Ana; Barbosa, Rui M; Laranjinha, Joao. "Regulation of Oxygen Consumption by Endogenous Nitric Oxide in the Hippocampus of a Triple Transgenic Model of Alzeimer´s Disease.". Trabalho apresentado em 8th FENS Forum of Neuroscience, 2012. Ledo, Ana; Barbosa, Rui M; Cadenas, Enrique; Laranjinha, Joao. "Endogenous nitric oxide governs oxygen consumption in the rat hippocampus". Trabalho apresentado em III International Symposium on the pathiphysiology of REactive Oxygen and Nitrogen Species, 2010. Ferreira, Nuno Ricardo; Lourenço, Cátia F; Santos, Ricardo; Ledo, Ana; Laranjinha, Joao; Barbosa, Rui M. "In vivo monitoring of nitric oxide in the rat brain: Characterization of a carbon fiber based probe". Trabalho apresentado em XVII Congresso Nacional de Bioquimica, 2010. Bento, Margaret; Ledo, Ana; Belo, José António. "Functional analysis of novel genes involved in the differentiation of heart/hemangioblast precursor cells (H/HPC)". Trabalho apresentado em Cardiac Development and Regeneration Symposium, 2008. Ledo, Ana; Barbosa, Rui M; Laranjinha, Joao. "Dynamic and Interacting Profiles of Oxygen and Nitric Oxide in the Hippocampus". Trabalho apresentado em 10th Meeting of Portuguese Society for Neurosciences, 2007. Ferreira, Nuno Ricardo; Ledo, Ana; Frade, João; Gerhardt, G.; Laranjinha, Joao. "Electrochemical Monitoring of Endogenously Produced Nitric Oxide in Hippocampal Brain Slices Using Carbon Fiber Microelectrodes". Trabalho apresentado em XVIII International Symposium on Bioelectrochemistry and Bioenergetics of the Bioelectrochemistry Society and 3rd Spring Meeting Bioelectrochemistry of the International Society of Electrochemistry, 2005. Ledo, Ana; Frade, João; Ferreira, Nuno Ricardo; Gerhardt, G.; Barbosa, Rui M; Laranjinha, Joao. "Oxygen Profiles in Acute Rat Hippocampal Slices - Regulation by NMDA Evoked Nitric Oxide Transients". Trabalho apresentado em XVIII International Symposium on Bioelectrochemistry and Bioenergetics of the Bioelectrochemistry Society and 3rd Spring Meeting Bioelectrochemistry of the International Society of Electrochemistry, 2005. Ledo, Ana; Frade, João; Ferreira, Nuno Ricardo; Barbosa, Rui M; Laranjinha, Joao. "Endogenously-produced nitric oxide in hippocampus modulates oxygen profiles". Trabalho apresentado em Oxidants and Antioxidants in Biology, Oxygen Club California Meeting, 2005. Frade, João; Ledo, Ana; Ferreira, Nuno Ricardo; Barbosa, Rui M; Laranjinha, Joao. "Ethanol modulation of nitric oxide production evoked by stimulation of glutamate receptors in hippocampal slices". Trabalho apresentado em Oxidants and Antioxidants in Biology, Oxygen Club California Meeting, 2005. Ledo, Ana; Frade, João; Ferreira, Nuno Ricardo; Barbosa, Rui M; Laranjinha, Joao. "From NMDA receptor activation to nitric oxide concentration dynamics". Trabalho apresentado em 4th Forum of European Neuroscience Societies, 2004. Ledo, Ana; Frade, João; Ferreira, Nuno Ricardo; Barbosa, Rui M; Laranjinha, Joao. "From NMDA Receptor Activation to Nitric Oxide Concentration Dynamics: a Study in Rat Hippocampal Slices". Trabalho apresentado em 4th Forum of European Neuroscience, 2004. Frade, João; Ledo, Ana; Ferreira, Nuno Ricardo; Barbosa, Rui M; Laranjinha, Joao; Ledo, Ana. "NMDAR- and AMPAR-dependent Production of Nitric Oxide in the CA1 Region of Hippocampal Slices: a Comparison". Trabalho apresentado em 4th Forum of European Neuroscience, 2004. Ledo, Ana; Frade, João; Ferreira, Nuno Ricardo; Barbosa, Rui M; Laranjinha, Joao. "Electrochemical Measuremente of Nitric Oxide Production in Rat Hippocampal Slices". Trabalho apresentado em 8th Meeting of the Portuguese Society for Neurosciences, 2003. Frade, João; Ledo, Ana; Barbosa, Rui M; Laranjinha, Joao. "Detection and Modulation of Nitric Oxide in Rat Hippocampus Using Modified Microelectrodes". Trabalho apresentado em 8th Meeting of the Portuguese Society for Neurosciences, 2003. Ferreira, Nuno Ricardo; Ledo, Ana; Frade, João; Laranjinha, Joao; Barbosa, Rui M. "Monitoring Nitric Oxide in Hippocampal Brain Slices Using Modified Carbon Fibre Microelectrodes". Trabalho apresentado em 3º Encontro Nacional de Química Analítica, 2003. Ferreira, Nuno Ricardo; Ledo, Ana; Frade, João; Laranjinha, Joao; Barbosa, Rui M. "Real Time Monitoring of Nitric Oxide in Rat Hippocampal Slices Using Carbon Fibre Microelectrodes". Trabalho apresentado em 1st Luso-Spanish Congress on Free Radicals and 6th Portuguese Congress on Free Radicals in Chemistry, Biology and Medicine, Coimbra, 2003. Frade, João; Ledo, Ana; Ferreira, Nuno Ricardo; Barbosa, Rui M; Laranjinha, Joao. "Modulation of Nitric Oxide Production/Decay in Rat Hippocampus by Caffeic Acid". Trabalho apresentado em 1st Luso-Spanish Congress on Free Radicals and 6th Portuguese Congress on Free Radicals in Chemistry, Biology and Medicine, 2003. Ledo, Ana; Barbosa, Rui M; Frade, João; Laranjinha, Joao. "Medição Electroquímica de Óxide Nítrico em Fatias de Hipocampo de Rato". Trabalho apresentado em VII Reunión del Grupo Español de Radicales Libres y III Reunión Iberoamericana, 2002. Ledo, Ana; Barbosa, Rui M; Frade, João; Laranjinha, Joao. "Selective Measurement of Nitric Oxide in Hippocampal Brain Slices Challenged with L-Glutamate". Trabalho apresentado em XIII Congresso Nacional de Bioquímica, 2002. Frade, João; Ledo, Ana; Barbosa, Rui M; Laranjinha, Joao. "Modulation of NO Concentration in Rat Hippocampal Brain Slices with Phenolic Antioxidants". Trabalho apresentado em XIII Congresso Nacional de Bioquímica, 2002. Ledo, Ana; Laranjinha, Joao; Barbosa, Rui M. "Electrochemical Detection of Nitric Oxide (NO) in Biological Media Using Modified Carbon Fiber Electrodes". Trabalho apresentado em XII Congresso Nacional de Bioquímica, 2000. Ledo, Ana; Laranjinha, Joao; Barbosa, Rui M. "Modified Carbon Electrodes for Electrochemical Detection of Nitric Oxide (NO) in Biological Media". Trabalho apresentado em II Congresso de Investigação em Medicina, 2000.
Resumo em conferência	Laranjinha, João; Lourenço, Cátia F.; Ledo, Ana; Dias, Cândida; Ferreira, Nuno Ricardo Esteves; Barbosa, Rui M.. "Redox regulation of neurovascular-neuroenergetic coupling axis by nitric oxide in animal models of aging and neurodegeneration". Trabalho apresentado em EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Coimbra, 2019. Publicado Ledo, Ana; Mancini, Gianni; Dias, Cândida; Lourenço, Cátia F.; Laranjinha, João; de Bem, Andreza. "Connecting the dots between a High-Fat/Cholesterol Diet and Sporadic Alzheimer's Disease". Trabalho apresentado em EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Coi8mbra, 2019. Publicado Ledo, Ana; Dias, Cândida; Lourenço, Cátia F.; Barbosa, Rui M.; Laranjinha, João. "Nitric oxide bioactivity in the hippocampus in aging and neurodegeneration". Trabalho apresentado em EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Coimbra, 2019. Publicado Monteiro, Tiago; Dias, Cândida; Lourenço, Cátia F.; Ledo, Ana; Laranjinha, João; Barbosa, Rui M.; Almeida, Maria G.. "Real-time detection of nitrite in the brain using carbon fibre microelectrodes". Trabalho apresentado em 2nd International Congress of CiiEM - Translational Research and Innovation in Human and Health Sciences, Almada, 2018. Publicado Lourenco, C. F.; Ledo, A.; Barbosa, R. M.; Laranjinha, J.. "DERAILMENT IN NITRIC OXIDE-DEPENDENT NEUROVASCULAR AND NEUROMETABOLIC COUPLING IN AGING". 2010. Publicado Lourenco, C. F.; Ledo, A.; Gerhardt, G. A.; Laranjinha, J.; Barbosa, R. B.. "OXIDASE-BASED MICROBIOSENSOR FOR REAL TIME MONITORING OF GLUCOSE AND NEURONAL ACTIVITY IN AWAKE BEHAVING ANIMALS". 2010.
Tese / Dissertação	Ledo, Ana. "Dinâmica de Concentração do Óxido Nítrico Produzido no Hipocampo de Rato por Activação de Receptores do Glutamato". Doutoramento, Universidade de Coimbra Faculdade de Ciencias e Tecnologia, 2007. https://estudogeral.sib.uc.pt/jspui/handle/10316/9660. Ledo, Ana. "Desenvolvimento de Microssensores para Medição Eletroquímica de Óxido Nítrico". Licenciatura, Universidade de Coimbra Faculdade de Ciencias e Tecnologia, 2001.

Atividades

Apresentação oral de trabalho

	Título da apresentação	Nome do evento Anfitrião (Local do evento)
2020/10	Diffusion and Removal Kinetics of Hydrogen Peroxide in Brain Tissue – a Study Using Ruthenium Purple Modified Carbon Fiber Microelectrodes	Analítica 2020 Sociedade Portuguesa de Química
2018/06/20	Keynote Lecture: Concurrent Recording of Neurometabolic Changes and Local Field Potential in the Central Nervous System of Awake-Behaving Rodent Models of Epilepsy¿.	3rd International Congress of the CiiEM - Research, Innovation in Human & Health Sciences. Egas Moniz Cooperativa de Ensino Superior (Costa da Caparica, Portugal)
2018/06/06	Age-dependent changes of neurovascular coupling and brain metabolism in Fischer 344 rats	19th Biennial Meeting of the Society for Free Radical Research (SFRRi2018) SFRRi (Lisbon, Portugal)
2018/06/04	High-fat and high cholesterol diet induces Alzheimer's like disease in mice	19th Biennial Meeting of the Society for Free Radical Research (SFRRi2018) SFRRi (Lisbon, Portugal)
2018/03/27	Age-dependent changes in the glutamate-nitric-oxide pathway in the hippocampus of the triple transgenic mouse model of Alzheimer’s disease: implications on mitochondrial function	Joint Meeting - WISe3 (Ludwig Boltzmann Institute for Experimental and Clinical Traumatology and AUVA Research Center; Ororboros Instruments and MitoFit Lab Institute of Surgical Research, Universidade de Szeged, Hungary (Szeged, Hungria)
2017/04/01	Neurometabolic and electrophysiological changes during cortical spreading depolarization: multimodal approach based on a lactate-glucose dual microbiosensor array.	28th Symposium on Cerebral Blood Flow, Metabolism and Function and 13th Conference on Quantification of Brain Function with PET Society for Cerebral Blood Flow and Metabolism (Berlin, Alemanha)
2017	Regulation of neurovascular coupling in the brain by nitrite	OCC World Congress and Annual SFRR-E Conference 2017 Metabolic Stress and Redox Regulation OCC - Oxygen Club California (Berlin, Alemanha)
2016/06/05	Platinum Microelectrode Arrays for High Spatio-temporal Resolution Recordings in the Brain: Surface Properties, Electrochemical Characteristics and In Vivo Analytical Performance	8th Meeting of the Analytical Chemistry Division Instituto Superior Técnico, Lisboa (Lisbon, Portugal)
2016/05/30	Enzyme-based microbiosensor arrays for high-resolution measurements of glucose and other metabolites in the brain	16th Conference of the International Society for Monitoring Molecules in Neuroscience Society for Monitoring Molecules in Neuroscience (Gothengurg, Suécia)
2016	ENZYME-BASED MICROBIOSENSOR ARRAYS FOR HIGH-RESOLUTION MEASUREMENTS OF GLUCOSE AND OTHER METABOLITES IN THE BRAIN	16th Conference of the International Society for Monitoring Molecules in Neuroscience International Society for Monitoring Molecules in Neuroscience (Gothenburg, Suécia)
2015/12/08	How a high-fat high cholesterol diet induces Alzheimer's disease like alterations in mice - evidence of cerebrovascular dysfunction	XIX National Conference of Biochemistry (SPB2016) Portuguese Society for Biochemistry (Guimarães, Portugal)
2015/07/07	Profiling Nitrergic Signaling Pathways in a Transgenic Mouse Model of Huntington’s Disease. Signaling Pathways and Novel Targets in Neurodegenerative Diseases	Satellite Symposium, IBRO 2015 IBRO (Rio de Janeiro, Brasil)
2015/05/13	Design of implantable enzyme-based microelectrodes for real-time measurements of neurochemicals and metabolic markers in vivo in the brain.”	4th International Conference on Bio-Sensing Technology (Lisbon, Portugal)
2015	Profiling Nitrergic Signaling Pathways in a Transgenic Mouse Model of Huntington’s Disease	Satellite Symposium‘ ‘Signaling Pathways and Novel Targets in Neurodegenerative Diseases.’ Brazilian Society for Neuroscience and Behavior (Rio de Janeiro, Brasil)
2015	Design of implantable enzyme-based microelectrodes for real-time measurements of neurochemicals and metabolic markers in vivo in the brain.	4th International Conference on Bio-Sensing Technology (Lisbon, Portugal)
2013/09/08	In Vivo Monitoring of Nitric Oxide and Other Relevant Neurochemicals in the Rat Brain Using Electrochemical Microsensor	64th Annual Meeting of the International Society of Electrochemistry International Society of Electrochemistry (Queratero, México)
2013	Oxygen and Nitric Oxide Concentration Profiles Linked to NMDA Receptor Activity in the Hippocampus of a Model of Alzheimer's Disease	Gordon Research Seminar on Oxidative Stress and Disease Gordon Research Conferences (Les Diablerets, Suiça)
2013	Dinâmicas de Concentração de Óxido Nítrico Endógeno no Sistema Nervoso Central: Desde Fatias a Modelos Animais de Neurodegenerescência	Seminar Series from the Graduate Program in Pharmacology Universidade Feredal de Santa Catarina, Departamento de Farmacologia (Florianópolis, Brasil)
2013	Concentration Dynamics of Nitric Oixde in Rat Hippocampal Subregions Evoked by Stimulation of the NMDA Receptor	Antônio Luiz Vianna Pós-Graduação do Instituto de Bioquímica Médica - UFRJ (Rio de Janeiro, Brasil)
2012/07/05	Endogenous Nitric Oxide Governs Oxygen Consumption in the rat Hippocampus	International Symposium on Redox Signalling and Oxidative Stress in Health and Disease (Valencia, Espanha)
2010	Neurovascular coupling in the brain mediated by nitric oxide. Implications in Alzheimer´s disease.	Joint Workshop of the COST Actions D34 and B35 on Oxidative stress and neurodegenerative diseases COST (Lisboa, Portugal)
2010	Monitoring Nitric Oxide Concentration Dynamics in a Transgenic Mouse Model of Huntington’s Disease	13th International Conference on In Vivo Methods (Bruxelas, Bélgica)
2010	Endogenous nitric oxide governs oxygen consumption in the rat hippocampus	III International Symposium on the Pathophysiology of Reactive Oxygen ans Nitrogen Species (Salamanca, Espanha)
2010	Molecular Targets of Endogenous nitric oxide in the rat hippocampus: regulation of mitochondrial respiration	XVII Congresso Nacional de Bioquímica Sociedade Portuguesa de Bioquimica (Porto, Portugal)
2008	In vivo dynamics of nitric oxide and oxygen changes in the rat brain	XVIth National Congress of Biochemistry Sociedade Portuguesa de Bioquímica (Ponta Delgada, São Miguel, Açores, Portugal)
2007	Dynamics of nitric oxide and oxygen changes in the hippocampus	FREE RADICALS IN MONTEVIDEO 2007 - V Meeting of SFRBM - South American Group and V International Conference on Peroxynitrite and Reactive Nitrogen Species V Meeting of SFRBM - South American Group (Montevideo, Uruguai)
2005	Modulation of NMDA evoked nitric oxide transients in the hippocampus by superoxide mimetic compounds	9ª Reunião Científica Nacional da Sociedade Portuguesa de Neurociências Sociedade Portuguesa de Neurociências (Luso, Portugal)
2005	Ethanol modulation of nitric oxide production in hippocampal slices	9ª Reunião Científica Nacional da Sociedade Portuguesa de Neurociências Sociedade Portuguesa de Neurociências (Luso, Portugal)
2005	Nitric oxide dynamics in hippocampus following activation of NMDA and AMPA receptors	Oxidants and Antioxidants in Biology, Oxygen Club of California Meeting Oxygen Club of California (Alba, Itália)
2004	Subregional Concentration Dynamics of Nitric Oxide in Hippocampal Slices Evoked by Stimulation of Glutamate NMDA Receptors	XII Biennial Meeting of the Society for Free Radical Research International, Buenos Aires Society for Free Radical Research International (Buenos Aires, Argentina)
2003	Selective Measurement of Nitric Oxide Production Evoked by Glutamate Agonists in Rat Hippocampal Brain Slices	Oxidants and Antioxidants in Biology (Cádiz, Espanha)
2003	Electrochemical Measurement of Nitric Oxide in Brain Slices Using o-Phenylenediamine-modified Carbon Fiber Microelectrode	1º Congresso da Sociedade Portuguesa de Ciências Farmacêuticas Sociedade Portuguesa de Ciências Farmacêuticas (Lisboa, Portugal)
2003	Real-time and Direct Measurement of Nitric Oxide in the Differente Subregions of Rat Hippocampal Slices Challenged with NMDA	1st Luso-Spanish Congress on Free Radicals and 6th Portuguese Congress on Free Radicals in Chemistry, Biology and Medicine (Coimbra, Portugal)
2002	“Real-time Monitoring of Nitric Oxide in Rat Hippocampal Brain Slices	Real-time Monitoring of Nitric Oxide in Rat Hippocampal Brain Slices (Paris, França)
2002	Selective Measurement of Nitric Oxide in rat Hippocampal Brain Slices	VII Reunión del Grupo Español de Radicales Libres y III Reunión Iberoamericana (Cáceres, Espanha)
2002	Nitric Oxide Signals in Hippocampal Brain Slices: Real-time Mapping and Modulation	XIII Congresso Nacional de Bioquímica Sociedade Portuguesa de Bioquímica (Lisboa, Portugal)
2001	Nitric Oxide Measurement in Hippocampal Brain Slices	5º Congresso de Radicais Livres em Química, Biologia e Medicina” Porto, Portugal (Porto, Portugal)
2000	Nitric Oxide Measurement in Biological Media	XXXI Reunião da Sociedade Portuguesa de Farmacologia e XIX Reunião de Farmacologia Clínica Sociedade Portuguesa de Farmacologia (Porto, Portugal)

Orientação

	Título / Tema Papel desempenhado	Curso (Tipo) Instituição / Organização
2023/01/01 - Atual	Design and development of implantable microelectrode-based sensors and biosensors for monitoring neurotransmitters and neurometabolic markers in vivo in the brain Coorientador	Doctoral Program in Pharmaceutical Sciences (Doutoramento) Universidade de Coimbra Faculdade de Farmácia, Portugal
2020/10/01 - Atual	Desenvolvimento e validação analítica de biossensores para medição de marcadores redox e/ou metabólicos em amostras clínicas Coorientador	Biomedicina Molecular (Mestrado) Universidade de Aveiro, Portugal
2019/02 - Atual	Construção de micro(bio)sensores baseados em microeléctrodos de fibra de carbono modificados com partículas e/ou enzimas oxidase imobilizadas, para monitorização da concentração de marcadores neurometabólicos Coorientador de Rita Durão Rosa Pereira	Bioquímica (Licenciatura/Bacharelato) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2016/01 - 2022/09/30	Dynamic changes of aerobic brain oxidative metabolism revealed by lactate and the redox modulation by nitrite Coorientador de Cândida Marília das Neves Dias	Ciências Farmacêuticas (Doutoramento) Universidade de Coimbra Faculdade de Farmácia, Portugal
2020/09/01 - 2022/09/26	Carbon nanotube modified carbon fiber microelectrodes for in vivo oximetry in an animal model of temporal lobe epilepsy Orientador de João Miguel Vieira	Engenharia Biomédica (Mestrado) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2021/09 - 2022/09/15	Personalized nutrition in the context of high performance sport: Biochemical Mechanisms Orientador	Ciências Farmacêuticas (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2020/10 - 2021/07	The Role of Diabetes in the Pathology of Alzheimer's Disease Orientador	Ciências Farmacêuticas (Outra) Universidade de Coimbra Faculdade de Farmácia, Portugal
2017/09 - 2018/06	Aplicação computacional para o processamento e análise de sinais de O2 recolhidos de modelos animais de epilepsia / Computational Software for the Processing and Analysis of Oxygen Signals Obtained in Animal Models of Epilepsy Coorientador de Maria José Mateus Branco	Engenharia Biomédica (Mestrado) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2013 - 2017	A Hipercolesterolemia como fator de risco na Doença de Alzheimer: impacto na biodisponibilidade e bioactividade do óxido nítrico Coorientador	Neurociencias (Doutoramento) Universidade Federal de Santa Catarina Centro de Ciências Biológicas, Brasil
2015 - 2015	Nitric oxide in Alzheimer's Disease - Signaling Molecule and Mediator of Pathogensis Orientador	Iniciação à Investigação (Iniciação científica) Centro de Neurociências e Biologia Celular, Portugal
2012 - 2013	High-Resolution Respirometry for Metabolic Profiling of Acute Rat Hippocampal Slices Coorientador de Tiago André Ferreira Henriques	Bioquímica (Mestrado) Faculdade de Ciências e Tecnologia, Universidade de Coimbra, Portugal

Organização de evento

	Nome do evento Tipo de evento (Tipo de participação)	Instituição / Organização
2018/10 - Atual	Simposium "The microbiota-gut-brain axis: a journey from dietary nitrate to nitric oxide via inorganic nitrite", accepted for the SFRR-Europe Ferrara 2019 Meeting, to take place 19-21 June 2019, Ferrara, Italy. (2019/06/19 - 2019/06/21) Simpósio (Coorganizador)	Society for Free Radical Research Europe, Reino Unido
2018/01 - Atual	19th Biennial Meeting of the Society for Free Radical Research (SFRRi2018) (2018/06/04 - 2018/06/07) Congresso (Membro da Comissão Organizadora)	Society for Free Radical Research - Europe, Reino Unido
2013 - Atual	4th PF2MUC Symposium “Personalized Medicine: Myth or Reality?” - Organization of Scientific Meeting integrated in the celebration of the Faculty of Medicine Day (University of Coimbra); organization of poster session and evaluation. (2013/10/25) Simpósio (Membro da Comissão Organizadora)	Universidade de Coimbra Faculdade de Medicina, Portugal
2009/05 - Atual	Organization of Laboratorial Course (2009/05) Outro	CNC.IBILI, Portugal
2022/08/22 - 2023/05/25	1st FEBS Redox Medicine Workshop: from cellular signaling to systems physiology and therapeutic targets (2023/05/22 - 2023/05/25) Oficina (workshop) (Coorganizador)	Universidade de Coimbra Faculdade de Farmácia, Portugal Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2013 - 2013	Grant Writing and Funding Opportunities Oficina (workshop) (Outra)	n.a., Portugal
2012 - 2012	2nd PF2MUC Symposium - Changing Paradigms in Science Simpósio (Outra)	Faculdade de Medicina da Universidade de Coimbra, Portugal
2009 - 2009	Vascular Biology Outro (Coorganizador)	Fundação para a Ciência e a Tecnologia, Portugal
2007 - 2007	SFRR Europe 2007 Meeting Congresso (Outra)	Fundação para a Ciência e a Tecnologia, Portugal

Júri de grau académico

	Tema Tipo de participação	Nome do candidato (Tipo de grau) Instituição / Organização
2023/04/14	Electrochemical immunosensors for detection of neurodegenerative diseases biomarkers Vogal	Pedro Jorge da Silva Carneiro (Doutoramento) Universidade do Porto Faculdade de Engenharia, Portugal
2022/09	Dynamic changes of aerobic brain oxidative metabolism revealed by lactate and the redox modulation by nitrite Orientador	Cândida Marília das Neves Dias (Doutoramento) Universidade de Coimbra Faculdade de Farmácia, Portugal
2022/09	A Potential Role for GLCM-based textural parameters in the Study of Alzheimer’s disease as measured by PiB PET images Presidente do júri	Filipe Lopes Amado (Mestrado) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2022/09	Carbon nanotube modified carbon fiber microelectrodes for in vivo oximetry in an animal model of temporal lobe epilepsy Orientador	João Miguel Fernandes Vieira (Mestrado) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2021/12	Internship Report and Monography entitled "Epigenetic therapy applied to cancer - new challenges in biomedicine" Arguente principal	Diana Soraia Ferreira Dias (Outro) Faculdade de Farmacia, Universidade de Coimbra, Portugal
2021/10	Bioanalytical challenges in detection and monitoring of hydrogen peroxide in redox signaling Arguente principal	João Pedro de Sousa Martins (Outro) Universidade de Coimbra Faculdade de Farmácia, Portugal
2021/07	Relatório de Estágio e Monografia intitulada "Utilização de biossensores na deteção precoce da Doença de Alzheimer" Arguente principal	Ana Catarina Gonçalves Amado (Outro) Faculdade de Farmacia, Universidade de Coimbra, Portugal
2021/07	Anti-dyslipidemic therapy in prevention of atherosclerotic cardiovascular diseases Arguente	Inês Alexandra da Silva Marçal (Outro) Universidade de Coimbra Faculdade de Farmácia, Portugal
2021	Anti-Inflammatory Action of Nitric Oxide in Inflammatory Bowel Disease Arguente principal	Fabiana Almeida Benigno (Outro) Universidade de Coimbra Faculdade de Farmácia, Portugal
2020/12	Exploring new biomarkers of aging Arguente principal	Inês de Sousa Oliveira (Outro) Universidade de Coimbra Faculdade de Farmácia, Portugal
2020/11/20	The Influence of Gut Microbiota in Neurodegenerative Diseases Arguente principal	Maria João de Sousa Sacramento (Outro) Faculdade de Farmacia, Universidade de Coimbra, Portugal
2020/11/06	Cerebrospinal fluid biomarkers in the diagnosis of Alzheimer's disease Arguente principal	Bruno Manuel Ferreira de Sousa (Outro) Faculdade de Farmacia, Universidade de Coimbra, Portugal
2020/09/15	Diagnosis of cardiac markers: the case of troponin I Presidente do júri	Ana Carolina Monteiro Costa (Outro) Faculdade de Farmacia, Universidade de Coimbra, Portugal
2020/09/14	Multiple Sclerosis: molecular mechanisms of the disease and therapeutic potencial with mesenchymal stem cells Arguente principal	Maria Inês Barroso Pereira da Costa (Mestrado) Faculdade de Farmacia, Universidade de Coimbra, Portugal
2020/07/13	Sleep and Insomnia: From molecular bases to new therapeutic approaches Arguente principal	Duarte Nuno Sampaio Loio (Mestrado) Faculdade de Farmacia, Universidade de Coimbra, Portugal
2019/09/19	In vivo monitoring of neurotransmitters and neuromodulators with microelectrode-based sensors and biosensors Arguente principal	Jessica Carina Simões Giraldo (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2019/09/09	Fecal Microbiota Transplant as a New Therapeutic Approach Arguente principal	Sara Santos Coimbra (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2019/02/22	Monitoring of ascorbate and glutamate in the brain with electrochemical sensors: application in the study of Huntington's disease Arguente principal	Daniela Sofia Barros Monteiro (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2018/09	Innovative pathways underlying the etiology and the therapeutics via modulation of the gut microbiota Arguente principal	Ricardo Manuel Esteves Ribeiro (Outro) Universidade de Coimbra Faculdade de Farmácia, Portugal
2018/07/27	As HDL e o Papel Benéfico na Prevenção da Aterosclerose: Estratégias Terapêuticas para o seu Aumento Arguente principal	Adriana Sofia Tomás (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2017/10/04	MicroRNAs e HDLs: Alvos Promissores na Terapêutica das Doenças Cardiovasculares? Arguente principal	Sara Andreia Zuzarte Cardoso (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2017/09/29	Motor excitability modulation during the delay period - a TMS and pupillometry study Arguente	Andreia Martins Almeida Verdade (Mestrado) Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2017	Mecanismos Epigenéticos na Formação de Memória e Capacidades Cognitivas Arguente principal	João António Lima de Andrade Conde Brazão (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2016	Neurovascular and neurmetabolic coupling in the brain: modulation by nitrite and its effects on neurodegeneration.	Cândida Marília das Neves Dias (Outro) Faculdade de Farmacia, Universidade de Coimbra, Portugal
2016	Neurovascular and neurometabolic coupling in the brain: modulation by nitrite and its effects on neurodegeneration	Cândida Marília das Neves Dias (Outro) Faculdade de Farmacia, Universidade de Coimbra, Portugal
2013	Neurovascular and Neurometabolic Coupling: Literature Review and Experimental approaches.	Miguel António Azinheira Caetano (Outro) Faculdade de Farmacia, Universidade de Coimbra, Portugal

Arbitragem científica em revista

	Nome da revista (ISSN)	Editora
2019 - Atual	Redox Biology (2213-2317)	Elsevier
2018 - Atual	Molecular Neurobiology (0893-7648)	Springer-Verlag
2018 - Atual	The Journal of Physiology (1469-7793)	Wiley (Blackwell Publishing)
2018 - Atual	Nitric Oxide (1089-8611)	Elsevier
2018 - Atual	Sensors (1424-8220)	MDPI AG
2017 - Atual	Experimental Gerontology	Elsevier
2017 - Atual	International Journal of Molecular Sciences (1422-0067)	MDPI AG
2017 - Atual	Molecules (1420-3049)	MDPI AG
2017 - Atual	Polymers (2073-4360)	MDPI AG
2017 - Atual	Genes (2073-4425)	MDPI AG
2017 - Atual	Nanomaterials (2079-4991)	MDPI AG
2017 - Atual	OBM Neurobiology	LIDSEN Publishing Inc
2017 - Atual	Biosensors	MDPI
2017 - Atual	Antioxidants (2076-3921)	MDPI AG
2017 - Atual	The Open Neurology Journal (1874-205X)	Bentham Science
2016 - Atual	Free radical Biology and Medicine	Elsevier
2015/10/27 - Atual	Oxidative Medicine and Cellular Longevity	Hindawi Publishing Corporation

Curso / Disciplina lecionado

	Disciplina	Curso (Tipo)	Instituição / Organização
2021/09 - Atual	Cell and Molecular Biology	Farmácia Biomédica (Licenciatura)	Universidade de Coimbra Faculdade de Farmácia, Portugal
2021/07 - Atual	Advanced Course	Bioanalytical Methods for Neurochemical Research (Curso de doutoramento (conclusão de unidades curriculares))	Faculdade de Farmacia, Universidade de Coimbra, Portugal
2021/02/15 - Atual	Biochemistry II	Ciências Farmacêuticas (Licenciatura)	Faculdade de Farmacia, Universidade de Coimbra, Portugal
2021/02/15 - Atual	Metabolism	Ciências Bioanalíticas (Licenciatura)	Faculdade de Farmacia, Universidade de Coimbra, Portugal
2021 - Atual	Metodologias de Laboratorio	Ciências Bioanalíticas (Licenciatura)	Universidade de Coimbra Faculdade de Farmácia, Portugal
2020/09 - Atual	Analytical Biochemistry	Licenciatura em Ciências Bioanalíticas (Licenciatura)	Faculdade de Farmacia, Universidade de Coimbra, Portugal
2020/09 - Atual	Cell Biology	Ciências Bioanalíticas (Licenciatura)	Faculdade de Farmacia, Universidade de Coimbra, Portugal
2020/09 - Atual	Cell Biology	Ciências Farmacêuticas (Mestrado integrado)	Faculdade de Farmacia, Universidade de Coimbra, Portugal
2020/09 - Atual	Cell Biology and Molecular Biology	Farmácia Biomédica (Licenciatura)	Faculdade de Farmacia, Universidade de Coimbra, Portugal
2015/09 - Atual	Practical Classes: Stereotaxic Surgery; Animal Handling-Mice; Sutures. Theoretical Classes: Rodent Biology; Basic Principals of Stereotaxy	Animal Science Course - Cat. C (Felasa Accredited) (Outros)	CNC.IBILI, Portugal
2012/01 - Atual	MIT-Portugal PhD Program - Neuroscience Module "Biosensors, NO and Neurovascular Coupling"	MIT-Portugal PhD Program (Curso de doutoramento (conclusão de unidades curriculares))	CNC.IBILI, Portugal
2017/02 - 2020/07	Biologia Molecular	Ciências Farmacêuticas (Mestrado integrado)	Universidade de Coimbra Faculdade de Farmácia, Portugal
2014 - 2014/10	Practical Classes: Handling and restraint of laboratory animals I and II; Anesthesia, analgesia and euthanasia II; Administration and Colection of Biological Samples I and II; Basic Principles of Surgery/Stereotaxy; Sutures; Necropsy.	Laboratory Animal Science - Cat. B (FELASA reccomended) (Outros)	CNC.IBILI, Portugal
2013 - 2013	Real-Time Electrochemical Measurements in the brain of living animals	Outra (Curso médio)	PDBEB Programme, 2012-2013 - Center for Neuroscience and Cell Biology, Portugal
2009 - 2009	Session 2: Modulation of Cytochrome c oxidase activity by nitric oxide	Extensão e divulgação científica (Curso médio)	CNC.IBILI, Portugal

Entrevista / Programa (rádio / tv)

	Programa	Tema
2017/02/13 - Atual	Antena 2 Ciência	Promoting the paper Dias, C. et al., “Age-dependent changes in the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic model of Alzheimer's disease: implications for neurometabolic regulation”. Neurobiol Aging. (2016) 46
2017/02/03 - Atual	Mentes que Brilham - Porto Canal	"Age-dependent changes in the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic model of Alzheimer's disease: implications for neurometabolic regulation"

Membro de associação

	Nome da associação	Tipo de participação
2018/03 - Atual	Physiological Society	Full member
2018/01 - Atual	Society for Free Radical Research - Europe	Full Member
2010 - Atual	Sociedade Portuguesa de Neurociências	Post-doctoral Member
2001 - Atual	Sociedade Portuguesa de Bioquímica

Revisão ad hoc de artigos em revista

	Nome da revista (ISSN)	Editora
2021 - Atual	Neurobiology of Disease	Elsevier
2015 - Atual	Journal of Visualized Experiments (JoVE)

Distinções

Prémio

2019	Leopold Flohe Redox Pioneer Young Investigator Award Society for Free Radical Research - Europe, Reino Unido
2014	Poster Prize
2010	Biochemical Journal Energy Poster Prize at the III International Symposium of the Pathophysiology of Reactive Oxygen and Nitrogen Species
2004	Prémio Estímulo à Investigação Fundação Calouste Gulbenkian, Portugal
2003	Travel Award

Outra distinção

2006	Research Highlight: Nature Reviews in Neuroscience for the publication in Proc. Natl. Acad. Sci. USA (Ledo et al. 2005, 102, 17483-88)