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Diana Sousa has a bachelor’s degree in biology and Geology (2019) and a M.Sc. degree in Biophysics and Bionanosystems concluded in 2022 both from the University of Minho. Her master's thesis was entitled "Optimization of nanoparticles for topical delivery of icilin in rheumatic diseases" and it was developed at the Bioprocess and Bionanotechnology Research Group at the Centre of Biological Engineering, University of Minho. Her work focused on the preparation, characterization and optimization of two types of nanoparticles, polymeric and liposomes. Throughout her career, Diana expanded her scientific skills. She is a self-sufficient user of distinct techniques across different areas, like: the development of nanoparticles (high-pressure homogenization, and ethanolic injection methods); characterization technics like Dynamic Light Scattering, Nuclear Magnetic Resonance, Fourier Transformed Infra-Red, differential scanning calorimeter, thermal gravimetric analysis, Circular dichroism). Diana as also expertise in drug release studies (dialysis bag method), transdermal permeation assay using Franz diffusion cells. Diana main interests are related with the interaction of compounds with distinct cells; therefore, she acquired expertise in cell culture maintenance and propagation. She started with by culturing and characterizing mono-cultures, moving to co-cultures and recently she is started to explore 3D in vitro cell models. The importance of a thorough characterization in well known, so Diana focused on the of distinct techniques to characterize the distinct cellular models, form the simplest ones Bradford assay and Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis, cellular viability to migration, flow cytometry (cell cycle and apoptosis), evaluation of the production of reactive oxygen species, as well as, the inflammatory response using ELISA kits. To success in her endevours Diana has attended and successfully concluded advanced courses in Bionanodevices for Clinic Diagnosis, Bionanosystems with Pharmaceutical and Dermacosmetic applications, and Functional and Intelligent Nanomaterials for Biomedical Applications, at the University of Minho. She also attended the course “Nutrition for Health and Sustainability”. Her qualifications earned her a position as a researcher in a project founded by a European Grant. The project consortium is composed by the University of Minho, University of Azores, Mesosystem S.S. company and the Norwegian food research institute Nofima (PT-INNOVATION-0096). Simultaneously, Diana has been involved in different projects that led to the publication of scientific papers: “Exploration of Polyphenols Extracted from Cytisus Plants and Their Potential Applications: A Review” (https://doi.org/10.3390/antiox13020192) and “Polymeric Biomaterials for Wound Healing” (https://doi.org/10.3389/fbioe.2023.1136077). Her extensive experimental work has a common base: the evaluation of natural compounds at a cellular level. The obtain results are under analysis to be submitted in less than month time. Diana presented her work at the AL4aninals Congress on Comparative and Translational Medicine and Biotechnology, in the format of an oral presentation. She also presented her work at the “Encontro Ciência 2023: Ciência e o Oceano Para Além do Horizonte” in the format of a poster. For the past two years, Diana has mentored several masters students. Two of them have already enrolled in a PhD program. Diana has collaborated in several science communication events, like Sientia@laboratorio, Noite Europeia dos Investigadores and “Universidade de portas abertas”.
Identificação

Identificação pessoal

Nome completo
DIANA ISABEL FERREIRA DE SOUSA

Nomes de citação

  • Ferreira-Sousa, Diana
  • Sousa, Diana

Identificadores de autor

Ciência ID
431F-5030-A600
ORCID iD
0000-0002-2252-6666

Endereços de correio eletrónico

  • id10389@alunos.uminho.pt (Profissional)
  • dianaifsousa@gmail.com (Pessoal)
  • dianaifsousa@hotmail.com (Pessoal)

Websites

Domínios de atuação

  • Ciências da Engenharia e Tecnologias - Nanotecnologia
  • Ciências Naturais - Ciências Biológicas - Biofísica
  • Ciências Naturais - Ciências Biológicas - Biologia
  • Ciências Naturais - Ciências Biológicas - Biologia Molecular
  • Ciências Naturais - Ciências Biológicas - Biologia Celular

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Francês Utilizador elementar (A2) Utilizador elementar (A2) Utilizador elementar (A2) Utilizador elementar (A2) Utilizador elementar (A2)
Inglês Utilizador independente (B2) Utilizador independente (B2) Utilizador independente (B2) Utilizador independente (B2) Utilizador independente (B2)
Espanhol; Castelhano Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1) Utilizador elementar (A1)
Formação
Grau Classificação
2022/09 - 2027
Em curso
Douturamento em Engenharia Biomédica (Doutoramento)
Universidade do Minho, Portugal
2023/01/20 - 2023/01/20
Frequentou
Segurança em Laboratório: Regras Gerais, Equipamentos de Proteção (EP's), Resíduos e Derrames (Outros)
Universidade do Minho Departamento de Biologia, Portugal
2019/09 - 2022/02
Concluído
Biophysics and Bionanosystems (Master)
Universidade do Minho, Portugal
"Optimization of nanoparticles for the topical delivery of icilin in rheumatic diseases" (TESE/DISSERTAÇÃO)
17
2020 - 2020
Concluído
Advanced course in Bionanodevices for Clinic Diagnosis (Outros)
Universidade do Minho, Portugal
18
2020 - 2020
Concluído
Advanced course in Bionanosystems with Pharmaceutical and Dermacosmetic applications (Outros)
Universidade do Minho, Portugal
17
2020 - 2020
Concluído
Advanced course in Functional and Intelligent Nanomaterials for Biomedical Applications (Outros)
Universidade do Minho, Portugal
16
2016/09 - 2019/06
Concluído
Biology and Geology (Bachelor)
Universidade do Minho, Portugal
13
Projetos

Bolsa

Designação Financiadores
2023/11/01 - 2024/09 Physalia Physalis - Innovative and unexploited source of high added-value cosmetic products
PT-INNOVATION-0096
Investigador
Universidade do Minho Centro de Engenharia Biológica, Portugal
EEA Grants
Em curso
Produções

Publicações

Artigo em revista
  1. Ferreira-Sousa, Diana; Zlatina Genisheva; María Jesús Rodríguez-Yoldi; Beatriz Gullón; Carlos E. Costa; José Teixeira; Claudia Botelho; Pedro Miguel Ferreira Santos. "Exploration of Polyphenols Extracted from Cytisus Plants and Their Potential Applications: A Review". Antioxidants (2024): http://dx.doi.org/10.3390/antiox13020192.
    10.3390/antiox13020192
  2. Oliveira, Cristiana; SOUSA, DIANA; Botelho, Claudia; Ferreira-Santos, Pedro; Teixeira, José A.. Autor correspondente: Botelho, Claudia. "Polymeric Biomaterials for Wound Healing". Frontiers in Bioengineering and Biotechnology (2023): http://journal.frontiersin.org/article/10.3389/fbioe.2023.1136077/full?&utm_source=Email_to_authors_&utm_medium=Email&utm_content=T1_11.5e1_author&utm_campaign=Email_publication&field=&journalName=Frontiers_in_Bioengineering_and_Biotechnology&id=1136077.
    Publicado
Poster em conferência
  1. Ferreira-Sousa, Diana. "Optimization of microave-assisted extraction for recovery of phenolic compounds from Cytisus flowers". Trabalho apresentado em 5th Iberoamerican congress on Biorefineries (5th-CIAB), 2024.
  2. Ferreira-Sousa, Diana. "Physalia physalis – Add Value to natural resources". Trabalho apresentado em Ciência 2023, 2023.
Tese / Dissertação
  1. "Optimization of nanoparticles for topical delivery of icilin in rheumatic diseases". Mestrado, Universidade do Minho, 2022.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2023/10/20 Natural bioactive compounds for the prevention of non-communicable diseases Comparative and translational medicine and biotechnology
ICBAS- Instituto de Ciências Biomédicas Abel Salazar (Porto, Portugal)

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação)
Instituição / Organização
2023/03/30 - 2023/04/01 Participation in the activity "Universidade Portas Abertas" with the perspective to make known the research that is carried out in the laboratories for future students, with guided tours to the facilities, workshops, and lectures. (2023/03/30 - 2023/04/01)
Outro (Outra)
Universidade do Minho - Campus de Azurém, Portugal
2023/02/08 - 2023/02/08 Collaborated in the organization of the activity "Scientia@Laboratórios (Scienctia@Laboratories)", an initiative organized by Scientia.com.pt/Experiment@Ciência. This activity aimed to talk to young people about our research and awaken their interest in science. (2023/02/08 - 2023/02/08)
Exposição (Membro da Comissão Organizadora)
Universidade do Minho, Portugal
2022/09/30 - 2022/09/30 Volunteered to join the initiative "Noite Europeia dos Investigadores" ( European Researchers' Night) which aims to bring schools and their students closer to research and science centers, showing them how research is conducted, and motivating them for science. This exchange and personal contact allows a better understanding of how research is developed and how knowledge is produced in my area. (2022/09/30 - 2022/09/30)
Outro (Coorganizador)
Universidade do Minho Escola de Ciências, Portugal
2017/09/29 - 2017/09/29 The European Researchers' Night is an initiative supported by the European Commission and takes place biannually, with the main objective of sharing with society the scientific research carried out in Portuguese institutions, in a relaxed and accessible way in order to break down the barriers that separate the Citizen science and demystify the image of the distant and inaccessible scientist. (2017/09/29 - 2017/09/29)
Exposição (Membro da Comissão Organizadora)
Universidade do Minho Escola de Ciências, Portugal

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2024/11/13 - 2024/11/13 In this activity I learned about the general benefits of performing in vitro experiments in 3D, plus got an in dept introduction to the solutions provided by ibidi. After the course, I aqquired advanced knowledge and a better understanding of the necessary tools needed to perform your own 3D cell culture experiments.
Oficina (workshop)
Cultivation of Cells in 3D
2024/09/11 - 2024/09/11 Cell migration is crucial for physiological processes like embryonic development, tissue repair, and wound healing but can become dysregulated in pathologies such as cancer metastasis and inflammation. Migration assays are essential for studying cell behavior and interactions under various conditions. This course provided an introduction to designing and conducting experiments to investigate cell migration, offering insights into its mechanisms and applications.
Oficina (workshop)
Introduction to Wound Healing and Migration Assays
2019/12/19 - 2019/12/20 I attended the event organized by the Master of Biophysics and Bionanosystems at the University of Minho with the objective of disseminating scientific knowledge. At the event, the work of several professionals in the area, including those of several former students, were publicized.
Simpósio
I Simpósio de Biofísica e Bionanossistemas
2019/03/11 - 2019/03/15 I attended the I Jornadas de Ciência, a week long event organized by students of the undergraduate degrees of the Biology Department . This activity featured several lectures by several nationally and internationally recognized researchers, multiple science-related workshops and a field trip.
Seminário
I Jornadas de Ciência
Universidade do Minho, Portugal
2017/03/18 - 2017/04/01 Contribution with a model under the theme of water, for the World WaterDay, exhibited in the "Sala deExposições da BLCS" and "QuintaPedagógica de Braga", in collaboration with the Scientia.com.pt project, from the School of Sciences of the University of Minho, and registered at UN-WATERof the United Nations. This activity was based on demonstrating inequalities in the distribution and access of water, as well as its management.
Exposição
Água p´ra que te quero… (Water forwhat I want you...)
Universidade do Minho, Portugal
2017/03/08 - 2017/03/08 I attended a lecture "A Biologia e a sua importância no quotidiano (Biology and its importance in everyday life)". This activity was part of the series of lectures organized by NEBGUM, with the aim of promoting interest in the various topics in the areas of both Biology and Geology.
Outro
Let´s talk about science
Universidade do Minho, Portugal

Tutoria

Tópico Nome do aluno
2024/04 - Atual Polyphenol-based nanoparticles for targeted therapies of prostate cancer Raquel Alexandra Gomes Fernandes
2023/03 - 2033/12 Valorization of by-products from the winery industry for the development of new cosmetic products Maria Beatriz Sanguedo Castro da Silva Oliveira
2024/04 - 2024/07 Natural Molecules as a Source of Nutraceuticals Ana Marta
2022/10 - 2024/01 Development of an osteosarcoma in vitro model Ana Carolina Ferreira Martins
2022/06 - 2023/10 Tailor-made Hyaluronic acid hydrogel for Dermocosmetics Cristiana Filipa Leite Oliveira
2022/06 - 2022/11 Simultaneous encapsulation of natural and synthetic therapeutic molecules into cubosomes for the treatment of osteosarcoma Miguel Pereira de Sousa
Distinções

Prémio

2013 Scholastic excellence award
Agrupamento de Escolas Santos Simões, Portugal
2012 Scholastic excellence award
Agrupamento de Escolas Santos Simões, Portugal