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Rui Barbosa is an associate professor at the Faculty of Pharmacy, University of Coimbra (FFUC), principal investigator at the Center for Neuroscience and Cell Biology (CNC-UC), and also affiliated with the Electroanalysis and Corrosion Laboratory of Institute Pedro Nunes. Currently, he is the Coordinator of the Laboratory of Analytical and Bioanalytical Methods and Coordinator of the Bioanalytical Sciences course of the FFUC. He obtained his Ph.D. in Pharmaceutical Chemistry in 1998 at FFUC. His research is now focused on the design and development of sensors and biosensors for in vivo and ex vivo detection and monitoring of neurochemicals based on microelectrode platforms. He has authored or co-authored more than 80 articles and reviews in specialized journals, counting ca. 2000 citations and an h-factor of 28. He has supervised or co-supervised several Post-Doc, Ph.D., and MSc students. He has had grants approved as Principal Investigator and participated in many other funded projects. Over the past decades, he has been a member of the management bodies of the FFUC and was vice-dean of the Faculty and the Library of Health Sciences of the University. In your Science Vitae curriculum, the most frequent terms in the context of scientific and technological production are pancreatic ß-cells, bursting electrical activity, pulsatile insulin release, nitric oxide microsensor, carbon nanotubes; in vivo electrochemistry, hippocampus, microelectrode arrays, carbon fiber microelectrodes, glucose, and lactate biosensor.
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Identificação

Identificação pessoal

Nome completo
Rui Manuel Silva Gomes Barbosa

Identificadores de autor

Ciência ID
5B16-6FC6-725F
ORCID iD
0000-0002-1604-6324

Endereços de correio eletrónico

  • rbarbosa@ff.uc.pt (Profissional)
  • rbarbosa@ci.uc.pt (Profissional)

Telefones

Telefone
  • 239488463 Ext.: 517614 (Profissional)

Moradas

  • Azinhaga de Santa Comba, Polo Ciências da Saúde- UC, 3000-548, Coimbra, Coimbra, Portugal (Profissional)

Websites

Domínios de atuação

  • Ciências Exatas - Química - Eletroquímica
  • Ciências Médicas e da Saúde - Biotecnologia Médica - Biotecnologia Aplicada à Saúde
Formação
Grau Classificação
1998
Concluído
 Farmácia (Doutoramento)
Especialização em Química Farmacêutica
Universidade de Coimbra Faculdade de Farmácia, Portugal
"Mecanismos Iónicos Subjacentes À Secreção Pulsátil de Insulina pelo Ilhéu de Langerhans" (TESE/DISSERTAÇÃO)
1987/03/01 - 1990/01/01
Concluído
 Provas de Aptidão Pedagógica e Capacidade Científica (Outros)
Especialização em Métodos Instrumentais de Análise
Universidade de Coimbra Faculdade de Farmácia, Portugal
1980/10/01 - 1986/07/31
Concluído
 Ciências Farmacêuticas (Licenciatura)
Especialização em Especialização em Farmácia Industrial
Universidade de Coimbra Faculdade de Farmácia, Portugal
 15.3
Percurso profissional

Docência no Ensino Superior

Categoria Profissional
Instituição de acolhimento 		Empregador
2017 - Atual Professor Associado (Docente Universitário) Universidade de Coimbra, Portugal
Universidade de Coimbra Faculdade de Farmácia, Portugal
1998/07/15 - 2017/03/23 Professor Auxiliar (Docente Universitário) Universidade de Coimbra, Portugal
Universidade de Coimbra Faculdade de Farmácia, Portugal

Cargos e Funções

Categoria Profissional
Instituição de acolhimento 		Empregador
2016 - 2018 Conselho geral ou orgão correspondente Universidade de Coimbra, Portugal
2016 - 2018 Coordenação ou direção de centro de investigação, departamento ou equivalente Universidade de Coimbra, Portugal
2013 - 2016 Conselho científico/técnico-científico ou orgão correspondente Universidade de Coimbra Faculdade de Farmácia, Portugal
2013 - 2016 Conselho de gestão ou orgão correspondente Universidade de Coimbra Faculdade de Farmácia, Portugal
2013 - 2016 Conselho geral ou orgão correspondente Universidade de Coimbra Faculdade de Farmácia, Portugal
Projetos

Projeto

Designação Financiadores
2023/03/01 - 2026/02/28 The Neurovascular Coupling as a biomarker and a target for cognitive enhancement in aging and vascular dementia through an innovative dietary nitrate-driven nitric oxide redox cycle: a translational study
2022.05454.PTDC
10.54499/2022.05454.PTDC
Universidade de Coimbra, Portugal
 Fundação para a Ciência e a Tecnologia
Em curso
2021/01/01 - 2025/12/31 Center for Innovative Biomedicine and Biotechnology - Associate Laboratory
LA/P/0058/2020
Universidade de Coimbra, Portugal
 Fundação para a Ciência e a Tecnologia
Em curso
2018/07/26 - 2021/07/25 Melhoria cognitiva no cérebro idoso e demência vascular em humanos através da funcionalização do acoplamento neurovascular: uma estratégia mecanística molecular promovida por nitrito e ácido ascórbico da dieta
PTDC/MED-NEU/29099/2017
Universidade de Coimbra, Portugal

Centro Hospitalar e Universitário de Coimbra EPE, Portugal

Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
2018/07 - 2021/07 Monitoring neurometabolic markers in vivo using microelectrode-based biosensors
POCI-01-0145-FEDER-28261
 Fundação para a Ciência e a Tecnologia
2019/01/01 - 2019/12/31 CNC. IBILI
UID/NEU/04539/2019
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Universidade de Coimbra, Portugal

Universidade de Coimbra Instituto de Ciências Nucleares Aplicadas à Saúde, Portugal

Universidade de Coimbra Instituto de Investigação Clínica e Biomédica de Coimbra, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2013/07/03 - 2015/09/30 Regulação do metabolismo energético no cérebro pelo óxido nítrico: solução para a glicólise aeróbia
PTDC/BBB-BQB/3217/2012
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluído
2011/03/01 - 2014/12/31 Nitrato:nitrito:óxido nítrico: uma via crítica que suporta o impacto benéfico do vinho e do azeite na fisiologia gastrointestinal e cardiovascular
PTDC/AGR-ALI/115744/2009
Universidade de Coimbra, Portugal

Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2010/05/01 - 2013/10/31 Acoplamento neurovascular entre a actividade neuronal e o fluxo sanguíneo no encéfalo mediado pelo óxido nítrico
PTDC/SAU-NEU/108992/2008
Universidade de Coimbra, Portugal

Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2010/05/01 - 2013/10/31 Design de sensores químicos e biossensores compósitos para a monitorização em tempo-real e em simultâneo de óxido nítrico e oxigénio in vivo no cérebro.
PTDC/SAU-BEB/103228/2008
Universidade de Coimbra, Portugal

Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2010/05 - 2013/10 Design of composite chemical sensors and biosensors for real-time and simultaneous monitoring of nitric oxide and oxygen in vivo in the brain.
Provided by PTCRIS: 103228
103228
 Fundação para a Ciência e a Tecnologia, I.P.
2010/06/01 - 2013/06/30 Perfis dinâmicos do óxido nítrico no cérebro: regulação da respiração celular com implicações para a doença de Alzheimer e para o envelhecimento
PTDC/SAU-NEU/103538/2008
Universidade de Coimbra, Portugal

Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2011/01/01 - 2012/12/31 Strategic Project - LA 1 - 2011-2012
PEst-C/SAU/LA0001/2011
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluído
2010/04 - 2012/03 Development of a micro-probe for capillary laser Doppler flowmetry in rat brain
PTDC/SAU-NEU/101726/2008
Universidade de Coimbra Departamento de Física, Portugal
 Fundação para a Ciência e a Tecnologia
Concluído
2008/01/01 - 2011/12/31 SenRONS - Desenvolvimento de sensores de fibra óptica para a determinação de espécies reactivas de oxigénio ROS e azoto RNS em sistemas biológicos.
PTDC/QUI/71001/2006
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Centro de Investigação em Química, Portugal

Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal

Instituto de Engenharia de Sistemas e Computadores, Portugal

Universidade de Coimbra, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
2007/05/15 - 2010/05/14 Novas funções biológicas de compostos fenólicos do vinho: regulação celular e acção anti-inflamatória via formação de óxido nítrico a partir de nitrito contido na dieta
PTDC/AGR-ALI/71262/2006
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluído
2002/03 - 2006/01 Real-time measurement of nitric oxide in hippocampal brain slices: modulation by tissue redox state with implications for neuronal degeneration
POCTI/BCI/42365/2001
Universidade de Coimbra Faculdade de Farmácia, Portugal

Universidade de Coimbra Instituto de Investigação Clínica e Biomédica de Coimbra, Portugal
Fundação para a Ciência e a Tecnologia
Concluído
Produções

Publicações

Artigo em conferência
  1. Candida Dias; Cátia F. Lourenço; Rui M. Barbosa; Ana Ledo; João Laranjinha. "Nitrite modulates mitochondrial respiration in brain ischemia/reperfusion". Trabalho apresentado em Annual Meeting of the Society-for-Free-Radical-Research- Europe (SFRRE) on Redox Homeostasis - From Signaling to Damage, 2019.
    Publicado
  2. Ana Ledo; Candida dias; Cátia F. Lourenço; Rui M. Barbosa; João Laranjinha. "Nitric oxide bioactivity in the brain - aging and disease". Trabalho apresentado em Annual Meeting of the Society-for-Free-Radical-Research- Europe (SFRRE) on Redox Homeostasis - From Signaling to Damage, Ferrara, 2019.
    Publicado
Artigo em revista
  1. Fonseca Carla; Cunha Susana; Ferreira Ricardo; Lapo Pais Mariana; Gonçalves Joana; Camins Antoni; Ettcheto Miren; Barbosa Rui; Ana Fortuna. "Cannabinoid Modulation of Monoamine Levels in Mouse Brain: Unveiling Neurochemical Dynamics through an Innovative High-performance Liquid Chromatography-Fluorescence Detection Bioanalysis". Journal of Analysis and Testing (2024): http://dx.doi.org/10.1007/s41664-024-00307-0.
    10.1007/s41664-024-00307-0
  2. Eliana Fernandes; Ana Ledo; Greg A. Gerhardt; Rui M. Barbosa. "Amperometric bio-sensing of lactate and oxygen concurrently with local field potentials during status epilepticus". Talanta (2024): https://doi.org/10.1016/j.talanta.2023.125302.
    10.1016/j.talanta.2023.125302
  3. Isabel Barbosa; Cátia Domingues; Rui M. Barbosa; Fernando Ramos. "Amanitins in Wild Mushrooms: The Development of HPLC-UV-EC and HPLC-DAD-MS Methods for Food Safety Purposes". Foods (2022): https://doi.org/10.3390/foods11233929.
    10.3390/foods11233929
  4. Cândida Dias; Eliana Fernandes; Rui M. Barbosa; Ana Ledo. "A Platinized Carbon Fiber Microelectrode-Based Oxidase Biosensor for Amperometric Monitoring of Lactate in Brain Slices". Sensors (2022): https://doi.org/10.3390/s22187011.
    10.3390/s22187011
  5. "In vivo hydrogen peroxide diffusivity in brain tissue supports volume signaling activity". Redox Biology 50 (2022): 102250-102250. http://dx.doi.org/10.1016/j.redox.2022.102250.
    10.1016/j.redox.2022.102250
  6. "Highly sensitive and selective nanostructured microbiosensors for glucose and lactate simultaneous measurements in blood serum and in vivo in brain tissue". Biosensors and Bioelectronics 199 (2022): 113874-113874. http://dx.doi.org/10.1016/j.bios.2021.113874.
    10.1016/j.bios.2021.113874
  7. Eliana Fernandes; Ana Ledo; Rui M. Barbosa. "Design and Evaluation of a Lactate Microbiosensor: Toward Multianalyte Monitoring of Neurometabolic Markers In Vivo in the Brain". Molecules (2022): https://doi.org/10.3390/molecules27020514.
    10.3390/molecules27020514
  8. Ledo, A.; Lourenço, C.F.; Cadenas, E.; Barbosa, R.M.; Laranjinha, J.. "The bioactivity of neuronal-derived nitric oxide in aging and neurodegeneration: Switching signaling to degeneration". Free Radical Biology and Medicine 162 (2021): 500-513. http://dx.doi.org/10.1016/j.freeradbiomed.2020.11.005.
    10.1016/j.freeradbiomed.2020.11.005
  9. Ledo, Ana; Fernandes, Eliana; Quintero, Jorge E.; Gerhardt, Greg A.; Barbosa, Rui M.. "Electrochemical Evaluation of a Multi-Site Clinical Depth Recording Electrode for Monitoring Cerebral Tissue Oxygen". Micromachines 11 7 (2020): 632. http://dx.doi.org/10.3390/mi11070632.
    10.3390/mi11070632
  10. Ledo, Ana; Fernandes, Eliana; Brett, Christopher M.A.; Barbosa, Rui M.. "Enhanced selectivity and stability of ruthenium purple-modified carbon fiber microelectrodes for detection of hydrogen peroxide in brain tissue". Sensors and Actuators B: Chemical 311 (2020): 127899. http://dx.doi.org/10.1016/j.snb.2020.127899.
    10.1016/j.snb.2020.127899
  11. Laranjinha, João; Nunes, Carla; Ledo, Ana; Lourenço, Cátia; Rocha, Bárbara; Barbosa, Rui M.. "The Peculiar Facets of Nitric Oxide as a Cellular Messenger: From Disease-Associated Signaling to the Regulation of Brain Bioenergetics and Neurovascular Coupling". Neurochemical Research 46 1 (2020): 64-76. http://dx.doi.org/10.1007/s11064-020-03015-0.
    10.1007/s11064-020-03015-0
  12. Lourenço, Cátia F.; Caetano, Miguel; Ledo, Ana; Barbosa, Rui M.. "Platinized carbon fiber-based glucose microbiosensor designed for metabolic studies in brain slices". Bioelectrochemistry 130 (2019): 107325. http://dx.doi.org/10.1016/j.bioelechem.2019.06.010.
    10.1016/j.bioelechem.2019.06.010
  13. Ana Ledo; Cátia F. Lourenço; João Laranjinha; Greg A. Gerhardt; Rui M. Barbosa. "Concurrent measurements of neurochemical and electrophysiological activity with microelectrode arrays: New perspectives for constant potential amperometry". Current Opinion in Electrochemistry 12 (2018): 129-140. https://doi.org/10.1016/j.coelec.2018.05.018.
    10.1016/j.coelec.2018.05.018
  14. Nuno R. Ferreira; Ana Ledo; João Laranjinha; Greg A. Gerhardt; Rui M. Barbosa. "Simultaneous measurements of ascorbate and glutamate in vivo in the rat brain using carbon fiber nanocomposite sensors and microbiosensor arrays". Bioelectrochemistry 121 (2018): 142-150. https://doi.org/10.1016/j.bioelechem.2018.01.009.
    10.1016/j.bioelechem.2018.01.009
  15. Cândida Dias; Cátia F. Lourenço; Rui M. Barbosa; João Laranjinha; Ana Ledo. "Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices". Analytical Biochemistry 551 (2018): 43-50. https://doi.org/10.1016/j.ab.2018.05.010.
    10.1016/j.ab.2018.05.010
  16. Cátia F. Lourenço; Ana Ledo; Greg A. Gerhardt; João Laranjinha; Rui M. Barbosa. "Neurometabolic and electrophysiological changes during cortical spreading depolarization: multimodal approach based on a lactate-glucose dual microbiosensor arrays". Scientific Reports (2017): https://doi.org/10.1038/s41598-017-07119-6.
    10.1038/s41598-017-07119-6
  17. Ana Ledo; Cátia F. Lourenço; João Laranjinha; Greg A. Gerhardt; Rui M. Barbosa. "Combined in Vivo Amperometric Oximetry and Electrophysiology in a Single Sensor: A Tool for Epilepsy Research". Analytical Chemistry (2017): https://doi.org/10.1021/acs.analchem.7b03452.
    10.1021/acs.analchem.7b03452
  18. Ana Ledo; Cátia F. Lourenço; João Laranjinha; Christopher M. A. Brett; Greg A. Gerhardt; Rui M. Barbosa. "Ceramic-Based Multisite Platinum Microelectrode Arrays: Morphological Characteristics and Electrochemical Performance for Extracellular Oxygen Measurements in Brain Tissue". Analytical Chemistry 89 3 (2017): 1674-1683. https://doi.org/10.1021/acs.analchem.6b03772.
    10.1021/acs.analchem.6b03772
  19. Egea, J.; Fabregat, I.; Frapart, Y.M.; Ghezzi, P.; Görlach, A.; Kietzmann, T.; Kubaichuk, K.; et al. "European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)". Redox Biology 13 (2017): 94-162. http://www.scopus.com/inward/record.url?eid=2-s2.0-85020032301&partnerID=MN8TOARS.
    10.1016/j.redox.2017.05.007
  20. Nunes, C.; Almeida, L.; Barbosa, R.M.; Laranjinha, J.. "Luteolin suppresses the JAK/STAT pathway in a cellular model of intestinal inflammation". Food and Function 8 1 (2017): 387-396. http://www.scopus.com/inward/record.url?eid=2-s2.0-85010953233&partnerID=MN8TOARS.
    10.1039/c6fo01529h
  21. Lourenço, C.F.; Ledo, A.; Barbosa, R.M.; Laranjinha, J.. "Neurovascular-neuroenergetic coupling axis in the brain: master regulation by nitric oxide and consequences in aging and neurodegeneration". Free Radical Biology and Medicine 108 (2017): 668-682. http://www.scopus.com/inward/record.url?eid=2-s2.0-85018262522&partnerID=MN8TOARS.
    10.1016/j.freeradbiomed.2017.04.026
  22. Lourenço, C.F.; Ledo, A.; Barbosa, R.M.; Laranjinha, J.. "Neurovascular uncoupling in the triple transgenic model of Alzheimer's disease: Impaired cerebral blood flow response to neuronal-derived nitric oxide signaling". Experimental Neurology 291 (2017): 36-43. http://www.scopus.com/inward/record.url?eid=2-s2.0-85011654048&partnerID=MN8TOARS.
    10.1016/j.expneurol.2017.01.013
  23. Dias, C.; Lourenço, C.F.; Ferreiro, E.; Barbosa, R.M.; Laranjinha, J.; Ledo, A.. "Age-dependent changes in the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic model of Alzheimer's disease: implications for neurometabolic regulation". Neurobiology of Aging 46 (2016): 84-95. http://www.scopus.com/inward/record.url?eid=2-s2.0-84978903965&partnerID=MN8TOARS.
    10.1016/j.neurobiolaging.2016.06.012
  24. Lourenço, C.F.; Ledo, A.; Laranjinha, J.; Gerhardt, G.A.; Barbosa, R.M.. "Microelectrode array biosensor for high-resolution measurements of extracellular glucose in the brain". Sensors and Actuators, B: Chemical 237 (2016): 298-307. http://www.scopus.com/inward/record.url?eid=2-s2.0-84976594136&partnerID=MN8TOARS.
    10.1016/j.snb.2016.06.083
  25. Santos, R.M.; Laranjinha, J.; Barbosa, R.M.; Sirota, A.. "Simultaneous measurement of cholinergic tone and neuronal network dynamics in vivo in the rat brain using a novel choline oxidase based electrochemical biosensor". Biosensors and Bioelectronics 69 (2015): 83-94. http://www.scopus.com/inward/record.url?eid=2-s2.0-84923082333&partnerID=MN8TOARS.
    10.1016/j.bios.2015.02.003
  26. Rocha, B.S.; Gago, B.; Barbosa, R.M.; Cavaleiro, C.; Laranjinha, J.. "Ethyl nitrite is produced in the human stomach from dietary nitrate and ethanol, releasing nitric oxide at physiological pH: Potential impact on gastric motility". Free Radical Biology and Medicine 82 (2015): 160-166. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925061584&partnerID=MN8TOARS.
    10.1016/j.freeradbiomed.2015.01.021
  27. Pereira, C.; Barbosa, R.M.; Laranjinha, J.. "Dietary nitrite induces nitrosation of the gastric mucosa: The protective action of the mucus and the modulatory effect of red wine". Journal of Nutritional Biochemistry 26 5 (2015): 476-483. http://www.scopus.com/inward/record.url?eid=2-s2.0-84928275461&partnerID=MN8TOARS.
    10.1016/j.jnutbio.2014.12.004
  28. Ferreira, N.R.; Lourenço, C.F.; Barbosa, R.M.; Laranjinha, J.. "Coupling of ascorbate and nitric oxide dynamics in vivo in the rat hippocampus upon glutamatergic neuronal stimulation: A novel functional interplay". Brain Research Bulletin 114 (2015): 13-19. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925442099&partnerID=MN8TOARS.
    10.1016/j.brainresbull.2015.03.002
  29. Lourenço, C.F.; Ledo, A.; Dias, C.; Barbosa, R.M.; Laranjinha, J.. "Neurovascular and neurometabolic derailment in aging and alzheimer's disease". Frontiers in Aging Neuroscience 7 MAY (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84931266835&partnerID=MN8TOARS.
    10.3389/fnagi.2015.00103
  30. Santos, R.M.; Lourenço, C.F.; Gerhardt, G.A.; Cadenas, E.; Laranjinha, J.; Barbosa, R.M.. "Erratum: Evidence for a pathway that facilitates nitric oxide diffusion in the brain (Neurochem. Int. (2011) 59 (90-96) DOI: 10.1016/j.neuint.2011.05.016)". Neurochemistry International 91 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84951179683&partnerID=MN8TOARS.
    10.1016/j.neuint.2015.11.004
  31. Lourenço, C.F.; Ferreira, N.R.; Santos, R.M.; Lukacova, N.; Barbosa, R.M.; Laranjinha, J.. "The pattern of glutamate-induced nitric oxide dynamics in vivo and its correlation with nNOS expression in rat hippocampus, cerebral cortex and striatum". Brain Research 1554 (2014): 1-11. http://www.scopus.com/inward/record.url?eid=2-s2.0-84895133025&partnerID=MN8TOARS.
    10.1016/j.brainres.2014.01.030
  32. Lourenço, C.F.; Santos, R.M.; Barbosa, R.M.; Cadenas, E.; Radi, R.; Laranjinha, J.. "Neurovascular coupling in hippocampus is mediated via diffusion by neuronal-derived nitric oxide". Free Radical Biology and Medicine 73 (2014): 421-429. http://www.scopus.com/inward/record.url?eid=2-s2.0-84903846214&partnerID=MN8TOARS.
    10.1016/j.freeradbiomed.2014.05.021
  33. Ledo, A.; Lourenço, C.F.; Caetano, M.; Barbosa, R.M.; Laranjinha, J.. "Age-Associated Changes of Nitric Oxide Concentration Dynamics in the Central Nervous System of Fisher 344 Rats". Cellular and Molecular Neurobiology 35 1 (2014): 33-44. http://www.scopus.com/inward/record.url?eid=2-s2.0-84921938863&partnerID=MN8TOARS.
    10.1007/s10571-014-0115-0
  34. Rocha, B.S.; Nunes, C.; Pereira, C.; Barbosa, R.M.; Laranjinha, J.. "A shortcut to wide-ranging biological actions of dietary polyphenols: Modulation of the nitrate-nitrite-nitric oxide pathway in the gut". Food and Function 5 8 (2014): 1646-1652. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904984090&partnerID=MN8TOARS.
    10.1039/c4fo00124a
  35. Santos, Ricardo M.; Rodrigues, Marcelo S.; Laranjinha, João; Barbosa, Rui M.; Santos, R.M.; Rodrigues, M.S.; Laranjinha, J.; Barbosa, R.M.. "Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain". Biosensors and Bioelectronics 44 1 (2013): 152-159. http://hdl.handle.net/10316/26914.
    10.1016/j.bios.2013.01.015
  36. Figueiras, Edite; Oliveira, Ricardo; Lourenço, Cátia F; Campos, Rita; Humeau-Heurtier, Anne; Barbosa, Rui M; Laranjinha, João; et al. "Self-mixing microprobe for monitoring microvascular perfusion in rat brain". Medical and Biological Engineering and Computing 51 1-2 (2013): 103-112. http://hdl.handle.net/10316/80012.
    10.1007/s11517-012-0973-x
  37. Pereira, C.; Ferreira, N.R.; Rocha, B.S.; Barbosa, R.M.; Laranjinha, J.. "The redox interplay between nitrite and nitric oxide: From the gut to the brain". Redox Biology 1 1 (2013): 276-284. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879005609&partnerID=MN8TOARS.
    10.1016/j.redox.2013.04.004
  38. Ferreira, N.R.; Santos, R.M.; Laranjinha, J.; Barbosa, R.M.. "Real Time In Vivo Measurement of Ascorbate in the Brain Using Carbon Nanotube-Modified Microelectrodes". Electroanalysis 25 7 (2013): 1757-1763. http://www.scopus.com/inward/record.url?eid=2-s2.0-84880348563&partnerID=MN8TOARS.
    10.1002/elan.201300053
  39. Rocha, B.S.; Gago, B.; Barbosa, R.M.; Lundberg, J.O.; Mann, G.E.; Radi, R.; Laranjinha, J.. "Pepsin is nitrated in the rat stomach, acquiring antiulcerogenic activity: A novel interaction between dietary nitrate and gut proteins". Free Radical Biology and Medicine 58 (2013): 26-34. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875974388&partnerID=MN8TOARS.
    10.1016/j.freeradbiomed.2012.12.017
  40. Nunes, C.; Ferreira, E.; Freitas, V.; Almeida, L.; Barbosa, R.M.; Laranjinha, J.. "Intestinal anti-inflammatory activity of red wine extract: Unveiling the mechanisms in colonic epithelial cells". Food and Function 4 3 (2013): 373-383. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874505366&partnerID=MN8TOARS.
    10.1039/c2fo30233k
  41. Oliveira, S.C.B.; Santarino, I.B.; Enache, T.A.; Nunes, C.; Laranjinha, J.; Barbosa, R.M.; Oliveira-Brett, A.M.. "Human colon adenocarcinoma HT-29 cell: Electrochemistry and nicotine stimulation". Bioelectrochemistry 94 (2013): 30-38. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879324150&partnerID=MN8TOARS.
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    10.1089/ars.2010.3297
  50. Rui M. Barbosa; Lopes Jesus, A. J; Ricardo M. Santos; Cassilda L. Pereira; Cátia F. Marques; Bárbara S. Rocha; Nuno R. Ferreira; Ana Ledo; João Laranjinha. "Preparation, standardization and measurement of nitric oxide solutions". Global Journal of Analytical Chemistry 2 (2011): 272-274. http://www.cognizure.com/abstract.aspx?p=101637271.
    Publicado
  51. Ledo, A.; Barbosa, R.; Cadenas, E.; Laranjinha, J.. "Dynamic and interacting profiles of .NO and O2 in rat hippocampal slices". Free Radical Biology and Medicine 48 8 (2010): 1044-1050. http://www.scopus.com/inward/record.url?eid=2-s2.0-77950516684&partnerID=MN8TOARS.
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  57. Lourenço, Cátia F.; Gago, Bruno; Barbosa, Rui M.; Freitas, Víctor de; Laranjinha, João. "LDL Isolated from Plasma-Loaded Red Wine Procyanidins Resist Lipid Oxidation and Tocopherol Depletion". (2008): http://hdl.handle.net/10316/10603.
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Livro
  1. Laranjinha, J.; Santos, R.M.; Lourenço, C.F.; Ledo, A.; Barbosa, R.M.. Nitric oxide signaling in the brain: Translation of dynamics into respiration control and neurovascular coupling. 2012.
    10.1111/j.1749-6632.2012.06582.x
  2. Ledo, A.; Barbosa, R.M.; Laranjinha, J.. Modulation of cellular respiration by endogenously produced nitric oxide in rat hippocampal slices. 2012.
    10.1007/978-1-61779-382-0_5
  3. Barbosa, R.M.; Lourenço, C.F.; Santos, R.M.; Pomerleau, F.; Huettl, P.; Gerhardt, G.A.; Laranjinha, J.. Chapter 20 In Vivo Real-Time Measurement of Nitric Oxide in Anesthetized Rat Brain. 2008.
    10.1016/S0076-6879(08)01220-2
  4. Ledo, A.; Barbosa, R.M.; Frade, J.; Laranjinha, J.. Nitric oxide monitoring in hippocampal brain slices using electrochemical methods. 2002.
    10.1016/S0076-6879(02)59176-X
  5. Santos, R.M.; Barbosa, R.M.; Antunes, C.M.; Silva, A.M.; Salgado, A.P.; Abrunhosa, A.J.; Pereira, F.C.; Seiça, R.M.; Rosário, L.M.. Bursting electrical activity generated in the presence of KATP channel blockers: Pharmacology, sensitivity to intracellular pH and modulation by glucose metabolism. 1997.
Pré-impressão
  1. Isabel Barbosa; Cátia Domingues; Fernando Ramos; Rui M. Barbosa. "Identification and quantification of a- and ß-amanitin in wild mushrooms by HPLC-UV-EC and HPLC-DAD-MS detection". 2022. https://doi.org/10.1101/2022.03.09.483521.
    10.1101/2022.03.09.483521
Resumo em conferência
  1. Ana Ledo; Eliana Fernandes; Christopher M.A. Brett; Rui M. Barbosa. "Diffusion and removal kinetics of hydrogen peroxide in brain tissue ¿ a study using ruthenium purple modified carbon fiber microelectrodes". Trabalho apresentado em Analítica 2020, 10th Meeting of the Analytical Division of Sociedade Portuguesa de Química, on-line, 2020, 2020.
    Publicado