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Joana Couceiro is a PhD student, enrolled in the PhD program in Biotechnology and Biosciences from Instituto Superior Técnico (IST), University of Lisbon (UL), since 2022. She is funded by an individual PhD scholarship from the highly competitive MIT Portugal program, and her dissertation focuses on the sustainable production of secondary metabolites from bacterial symbionts of octocorals. In the scope of her PhD, Joana described two novel marine bacterial species in the genus Aquimarina, A. aquimarini sp. nov. and A. spinulae sp. nov., published in the International Journal of Systematic and Evolutionary Microbiology (IJSEM). She also wrote a book chapter on bioactive natural products and biotechnological applications of the coral microbiome for the Springer book titled 'Coral Reef Microbiome', currently in press. Joana has also developed experimental work where she applies metabolomics and transcriptomics to test if co-cultures of sympatric marine bacteria enhances secondary metabolite production in the peptide antibiotic producing bacterium Aquimarina aquimarini, and a manuscript is currently in preparation. In her current experimental work, Joana is establishing a culture collection of bacteria associated with the temperate octocoral Leptogorgia sarmentosa. The collection encompasses ca. 400 isolates from three different oxygen conditions (aerobic, microaerophilic and anaerobic) which will later be tested for their antimicrobial and antifouling capacities. The prokaryotic diversity hosted by this octocoral will further be explored employing 16S rRNA amplicon sequencing. Joana obtained her Integrated Master's degree in Biological Engineering from IST-UL in January 2021. Her Master thesis at the Institute for Bioengineering and Biosciences (iBB/IST) focused on the diversity and structure of bacterioplankton communities in ice and water samples from the Subarctic, using metagenomics tools, and was rated excellent (18 on a 0-20 scale). Her MSc thesis work contributed to a research article published in the Journal "Science of the Total Environment" and an iPoster presented at the World Microbe Forum (ASM/FEMS). Between her Master and PhD studies, Joana was hired as research assistant on the R&D project SymbioReactor, funded through the FundoAzul program of the Portuguese Ministry of the Sea (DPGM). Here she acquired scientific training in marine genomics, annotating symbiosis-related genes and secondary metabolite biosynthesis gene clusters on the genomes of Roseibium album (Alphaproteobacteria) and Aquimarina megaterium (Flavobacteriia) strains isolated from octocorals. This work was published in two articles in the ASM journal "Microbiology Resource Announcements", both with Joana as first author. During her time as a Bachelor student, in summer 2017, Joana performed an internship in Microbial Biotechnology at the Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Beja, investigating bacterial tolerance to inhibitors derived from plant biomass hydrolysis. From August 2018 to January 2019, she gained international experience as an Erasmus exchange student at the KTH Royal Institute of Technology, in Stockholm, Sweden, taking courses on Drug Development and Microbial Cultivation in Bioreactors. From 2019 to the beginning of 2020, Joana was part of a student team developing a design project which won the 3rd ESBES-BIOT Biotechnical Design Competition, with a project entitled 'Continuous production of lentivirus for use in gene and/or cell therapy products'. So far, Joana has authored/co-authored five research articles and presented her work in over 10 poster and oral presentations in multiple national and international conferences.
Identificação

Identificação pessoal

Nome completo
Joana Andreia Fernandes Couceiro

Nomes de citação

  • Couceiro, Joana F.

Identificadores de autor

Ciência ID
8D1E-D651-4AAC
ORCID iD
0000-0003-1824-5017
Google Scholar ID
uiBfPXoAAAAJ

Endereços de correio eletrónico

  • joana.couceiro@tecnico.ulisboa.pt (Profissional)

Domínios de atuação

  • Ciências Naturais - Ciências Biológicas - Microbiologia
  • Ciências da Engenharia e Tecnologias - Biotecnologia Industrial - Tecnologias de BioProcessamento
  • Ciências Naturais - Ciências Biológicas - Bioquímica

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1)
Formação
Grau Classificação
2022/05/11 - 2026/05
Em curso
PhD program in Biotechnology and Biosciences (Doutoramento)
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2015/09/11 - 2021/01
Concluído
Engenharia Biológica (Mestrado integrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Discovering the polar microbiome: structure and diversity of prokaryotic communities in ice and seawater along a salinity gradient in the Subarctic " (TESE/DISSERTAÇÃO)
15 (Bachelor 15, Master 16)
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2022/01/01 - 2022/05/11 Assistente de Investigação (carreira) (Investigação) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2020/02 - 2021 Estagiário de Investigação (Investigação) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2017/07/03 - 2017/09/15 Estagiário de Investigação (Investigação) Centro de Biotecnologia Agrícola e Agro-Alimentar do Alentejo, Portugal

Outros

Categoria Profissional
Instituição de acolhimento
Empregador
2022/05/11 - Atual PhD Student Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2018/08 - 2019/01 Erasmus Exchange Student Kungliga Tekniska Högskolan, Suécia
Kungliga Tekniska Högskolan, Suécia
Projetos

Bolsa

Designação Financiadores
2023/02/01 - Atual Blue Bioeconomy Pack (PBA) - Recovery and Resilience Plan (PRR); Active on Work Package WP9 - Portuguese Blue Biobank
C632741873-00467082
Bolseiro de Doutoramento
Em curso
2022/05 - 2022/12 Symbioreactor - Sustainable production of bioactive metabolites from microbial symbionts of marine sponges and corals
FA05-2017-032
Bolseiro de Doutoramento
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Concluído
2022/01 - 2022/05 Symbioreactor - Sustainable production of bioactive metabolites from microbial symbionts of marine sponges and corals
FA05-2017-032
Bolseiro de Técnico de Investigação
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Concluído
Produções

Publicações

Artigo em revista
  1. Couceiro, Joana F.; Fernandes, Ana S.; Costa, Rodrigo; Keller-Costa, Tina. "Co-cultivation of bacterial competitors enhances secondary metabolite biosynthesis in the marine bacterium Aquimarina aquimarini.". FEMS Microbiology Ecology (2025):
  2. Couceiro, Joana F.; Marques, Matilde; Silva, Sandra G.; Keller-Costa, Tina; Costa, Rodrigo. "Aquimarina aquimarini sp. nov. and Aquimarina spinulae sp. nov., novel bacterial species with versatile natural product biosynthesis potential isolated from marine sponges". International Journal of Systematic and Evolutionary Microbiology 74 1 (2024): http://dx.doi.org/10.1099/ijsem.0.006228.
    10.1099/ijsem.0.006228
  3. Roveta, Camilla; Calcinai, Barbara; Girolametti, Federico; Fernandes Couceiro, Joana; Puce, Stefania; Annibaldi, Anna; Costa, Rodrigo. "The prokaryotic community of Chondrosia reniformis Nardo, 1847: from diversity to mercury detection". Zoology 158 (2023): 126091. http://dx.doi.org/10.1016/j.zool.2023.126091.
    10.1016/j.zool.2023.126091
  4. Couceiro, Joana Fernandes; Keller-Costa, Tina; Kyrpides, Nikos C.; Woyke, Tanja; Whitman, William B.; Costa, Rodrigo. "Genome Sequencing Suggests Diverse Secondary Metabolism in Coral-Associated Aquimarina megaterium". Microbiology Resource Announcements 11 11 (2022): http://dx.doi.org/10.1128/mra.00620-22.
    10.1128/mra.00620-22
  5. Coelho, Lígia Fonseca; Couceiro, Joana Fernandes; Keller-Costa, Tina; Valente, Sara Martinez; Ramalho, Tiago Pereirinha; Carneiro, Joana; Comte, Jérôme; et al. "Structural shifts in sea ice prokaryotic communities across a salinity gradient in the subarctic". Science of The Total Environment 827 (2022): 154286. http://dx.doi.org/10.1016/j.scitotenv.2022.154286.
    10.1016/j.scitotenv.2022.154286
  6. Couceiro, Joana Fernandes; Keller-Costa, Tina; Marques, Matilde; Kyrpides, Nikos C.; Woyke, Tanja; Whitman, William B.; Costa, Rodrigo. "The Roseibium album (Labrenzia alba) Genome Possesses Multiple Symbiosis Factors Possibly Underpinning Host-Microbe Relationships in the Marine Benthos". Microbiology Resource Announcements 10 34 (2021): http://dx.doi.org/10.1128/mra.00320-21.
    Acesso aberto • 10.1128/mra.00320-21
Capítulo de livro
  1. Couceiro, Joana F.; Costa, Rodrigo; Keller-Costa, Tina. "Chapter 16 - Beyond Restauration: Coral Microbiome Biotechnology". In Coral Reef Microbiome, editado por Raquel Peixoto; Christian R. Voolstra. Springer, 2024.
    No prelo
Poster em conferência
  1. Couceiro, Joana F.; Costa, Rodrigo; Keller-Costa, Tina. "Pharmaceutical and Biotechnological Applications of the Coral Microbiome". Trabalho apresentado em MICROBIOTEC'23 - Congress of Microbiology and Biotechnology, 2023.
  2. Fernandes, Ana M.; Couceiro, Joana F.; Keller-Costa, Tina; Costa, Rodrigo. "Exploring the potential of co-cultures to enhance secondary metabolites production in the marine bacterium Aquimarina sp. Aq135". Trabalho apresentado em MICROBIOTEC'23 - Congress of Microbiology and Biotechnology, 2023.
  3. Couceiro, Joana F.; Costa, Rodrigo; Keller-Costa, Tina. "Pharmaceutical and Biotechnological Applications of the Coral Microbiome". Trabalho apresentado em 3rd iBB workshop, 2023.
  4. Couceiro, Joana F.; Costa, Rodrigo; Keller-Costa, Tina. "Pharmaceutical and Biotechnological Applications of the Coral Microbiome". Trabalho apresentado em 2nd Microbiome Summit, 2023.
  5. Couceiro, Joana F.; Costa, Rodrigo; Keller-Costa, Tina. "Pharmaceutical and Biotechnological Applications of the Coral Microbiome". Trabalho apresentado em MIT Portugal Annual Conference, 2023.
  6. Couceiro, Joana F.; Marques, Matilde; Silva, Sandra G.; Keller-Costa, Tina; Costa, Rodrigo. "Aquimarina aquimarinae sp. nov. and Aquimarina spinosulus sp. nov., new bacterial species with versatile natural product biosynthesis potential". Trabalho apresentado em 16th Symposium on Bacterial Genetics and Ecology, 2023.
  7. Couceiro, Joana F.; Costa, Rodrigo; Keller-Costa, Tina. "Sustainable Production of Bioactive Metabolites from Octocoral Symbionts". Trabalho apresentado em SymbNET Metabolomics Workshop for Biologists, 2023.
  8. Couceiro, Joana F.; Marques, Matilde; Silva, Sandra G.; Keller-Costa, Tina; Costa, Rodrigo. "Aquimarina aquimarinae sp. nov. and Aquimarina spinosulus sp. nov., new bacterial species with versatile natural product biosynthesis potential". Trabalho apresentado em MIT Portugal Annual Conference, 2022.
  9. Roveta, Camilla; Annibaldi, Anna; Calcinai, Barbara; Coppari, M; Di Camillo, C.G; Girolametti, Federico; Puce, S.; et al. "Heavy meals for a light sponge. Mercury bioaccumulation in the sponge Chondrosia reniformis Nardo, 1847 and its prokaryotic community". Trabalho apresentado em 11th World Sponge Conference, Leiden, The Netherlands, 2022.
  10. Couceiro, Joana F.; Keller-Costa, Tina; Marques, Matilde; Kyrpides, Nikos C.; Woyke, Tanja; Whitman, William B.; Costa, Rodrigo. "The Roseibium album (Labrenzia alba) pangenome reveals multiple symbiosis factors likely underpinning host-microbe relationships in the marine benthos". Trabalho apresentado em MICROBIOTEC'21 - Congress of Microbiology and Biotechnology, 2021.
  11. Coelho, Lígia F.; Couceiro, Joana F.; Keller-Costa, Tina; Valente, Sara; Ramalho, Tiago; Carneiro, Joana; Comte, Jerôme; et al. "Structural shifts revealed for seawater and ice microbiomes across a salinity gradient in the high Subarctic". Trabalho apresentado em World Microbe Forum, 2021.
Tese / Dissertação
  1. Couceiro, Joana F.. "Discovering the polar microbiome: structure and diversity of prokaryotic communities in ice and seawater along a salinity gradient in the Subarctic". Mestrado, Universidade de Lisboa Instituto Superior Técnico, 2021.
Tradução
  1. Marques, Matilde; Couceiro, Joana F.; Keller-Costa, Tina. Coral Heroes. SECORE. 2023.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2022/10/18 Aquimarina aquimarinae sp. nov. e Aquimarina spinosulus sp. nov., novas espécies bacterianas com potencial biossintético de produtos naturais Microbiologia 2022
Sociedade Portuguesa de Microbiologia (SPM)

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação)
Instituição / Organização
2016 - 2019 Collaborator at the Bioengineering Department Open Labs (2016, 2018, 2019) (2016)
Exposição (Membro da Comissão Organizadora)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018 - 2018 Collaborator at the Chemical Engineering Department Open Labs (2018) (2018)
Exposição (Membro da Comissão Organizadora)
Universidade de Lisboa Instituto Superior Técnico, Portugal

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2024/02/06 - 2024/02/06 Workshop on the use and application of cluster computing systems for Big Data.
Oficina (workshop)
Course on Computational Biosciences using HPC systems
Unidade de Ciências Biomoleculares Aplicadas, Portugal
2021/09/17 - 2023/09/17 International Microorganism Day - IMD, yearly since 2021 exhibitor
Exposição
International Microorganism Day
Universidade de Lisboa Instituto Superior Técnico, Portugal
2023/06/06 - 2023/06/08 Application of metabolomics to the study of host-microbiome interactions.
Oficina (workshop)
SymbNET Metabolomics Workshop for Biologists
Université de Lausanne, Suiça

Instituto Gulbenkian de Ciência, Portugal
2021/05/23 - 2023/05/23 Dia do Técnico exhibitor; yearly since 2021 engagement with the public and school classes, showcasing 'The wonderful world of marine bacteria' 'The symbionts of corals and sponges' and 'Life in a drop of water'
Exposição
Dia do Técnico / Técnico Open Day
Universidade de Lisboa Instituto Superior Técnico, Portugal
2023/05 - 2023/05 Introduction to R utilization in Biological sciences (WS1 – introduction to R, WS2 – biostatistical basis in R and WS4 – single cell) Olissipo | EMBL training Introduction and computation with R
Instituto de Engenharia de Sistemas e Computadores Investigação e Desenvolvimento em Lisboa, Portugal
2022/04/04 - 2022/04/08 Overview of Python and R languages with an emphasis on biology related practical problem solving, focused on data plotting and reproducibility.
Oficina (workshop)
Introduction to R and Python for Biologists
Wellcome Sanger Institute, Reino Unido
2021/09/06 - 2021/09/10 35 hours of theoretical and field training on environmental sampling techniques commonly used for water, sediments, soils and biological samples
Oficina (workshop)
1st course of environmental sampling
Universidade de Lisboa Centro de Química Estrutural, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal

Membro de associação

Nome da associação Tipo de participação
2021 - Atual SPM - Sociedade Portuguesa de Microbiologia Member
2017 - 2020 Tuna Feminina do Instituto Superior Técnico Member (2017-2019) and President (2020)
2017 - 2018 Núcleo de Engenharia Biológica do Instituto Superior Técnico Collaborator

Tutoria

Tópico Nome do aluno
2016 - 2018 IST Mentoring program (2016-2018) First Year Students
Distinções

Prémio

2021 ESBES-BIOT Biotechnical Design Competition winner with the project 'Continuous production of lentivirus for use in gene and/or cell therapy products'
American Chemical Society, Estados Unidos

Outra distinção

2022 Honorable mention - oral presentation at Microbiologia'22
Sociedade Portuguesa de Microbiologia, Portugal
2022 Poster shortlisting - 25 best posters at MIT Portugal Annual Conference 2022
Massachusetts Institute of Technology MIT Portugal Program, Portugal