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Eduardo Pinho Melo. Concluiu o(a) Título de Agregado em Agregação em Biotecnologia em 2007 pelo(a) Universidade de Lisboa Instituto Superior Técnico, Doutoramento em Biotecnologia em 1995 pelo(a) Universidade de Lisboa Instituto Superior Técnico, Doutoramento em Biotecnologia em 1995 pelo(a) Universidade de Lisboa Instituto Superior Técnico e Licenciatura em Biologia Vegetal Aplicada em 1989 pelo(a) Universidade de Lisboa Faculdade de Ciências. Publicou 52 artigos em revistas especializadas. Atua na(s) área(s) de Ciências Naturais com ênfase em Ciências Biológicas.
Identification

Personal identification

Full name
Eduardo Pinho Melo

Citation names

  • Melo, Eduardo
  • Melo, Eduardo P

Author identifiers

Ciência ID
3C1C-C10C-1510
ORCID iD
0000-0002-0974-8977

Telephones

Mobile phone
  • 938549138 (Personal)

Addresses

  • Campus de Gambelas, 8005-139, Faro, Faro, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
French Intermediate (B1) Intermediate (B1) Upper intermediate (B2) Intermediate (B1)
Spanish; Castilian Intermediate (B1) Intermediate (B1) Intermediate (B1) Upper intermediate (B2) Intermediate (B1)
Education
Degree Classification
2007
Concluded
 Agregação em Biotecnologia (Título de Agregado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Estabilidade, Folding e Misfolding de Proteínas" (THESIS/DISSERTATION)
 Aprovado por Maioria
1995
Concluded
 Biotecnologia (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Actividade e Fluorescência de uma cutinase recombinante: Estudos em micelas invertidas e com a técnica da monocamada" (THESIS/DISSERTATION)
 Distinção e Louvor
1989
Concluded
 Biologia Vegetal Aplicada (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
"Actividade celulásica de fungos" (THESIS/DISSERTATION)
 16 valores
Affiliation

Science

Category
Host institution 		Employer
2017 - 2018 Visiting Researcher (Research) University of Cambridge Clinical Biochemistry, United Kingdom
University of Cambridge Clinical Biochemistry, United Kingdom
2010 - 2011 Visiting Researcher (Research) New York University, United States
New York University, United States
2002 - 2003 Visiting Researcher (Research) UniversitätsSpital Zürich Institut für Neuropathologie, Switzerland
UniversitätsSpital Zürich Institut für Neuropathologie, Switzerland

Teaching in Higher Education

Category
Host institution 		Employer
2003 - Current Associate Professor (University Teacher) Universidade do Algarve, Portugal
Universidade do Algarve, Portugal
1995 - 2003 Assistant Professor (University Teacher) Universidade do Algarve, Portugal
Universidade do Algarve, Portugal
Projects

Grant

Designation Funders
2022/01/01 - 2023/06/30 SMARTCELL - Cryopreservation of induced pluripotent stem cells
47169
Co-Principal Investigator (Co-PI)
Centro de Ciências do Mar, Portugal
Concluded

Contract

Designation Funders
2017 - 2018 Desenvolvimento de Tecnologia Criogénica para Substâncias Biológicas
Cryocube - Portugal 2020
Researcher
Universidade do Algarve, Portugal
Concluded
2013/04 - 2015/03 Functionalized Metal Nano-Gaps for Plasmon-Enhanced Single Protein Biosensing
Provided by PTCRIS: 128608
PTDC/CTM-NAN/2700/2012
128608
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2012/02 - 2015/01 Bioprospecting function of an ancient gene (CRTAC) encoding a novel protein in a marine teleost by comparative analysis
122296
Provided by PTCRIS: 122296
PTDC/MAR/12296/2010
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2012/02 - 2015/01 A Biophysical Approach to Oxidative Protein Folding in the ER: Relative Importance of Ero1 and Prdx4 alternative pathways
Provided by PTCRIS: 119677
119677
PTDC/QUI-BIQ/119677/2010
Principal investigator
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2010/01 - 2013/03 Controlled freezing to improve the stability and delivery of therapeutic proteins
Provided by PTCRIS: 104318
104318
PTDC/EQU-EQU/104318/2008
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2008/04 - 2012/03 Single Molecule Signalling of Peptide Metal Binding
Provided by PTCRIS: 64658
64658
PTDC/QUI/64658/2006
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2008/04 - 2011/12 Single-Molecule Detection of the Prion Protein: Characterization of Conformational Change Involved on Prion Diseases
Provided by PTCRIS: 73027
73027
PTDC/QUI/73027/2006
Principal investigator
Fundação para a Ciência e a Tecnologia, I.P.
2008/03 - 2011/12 Photoinduced electron transfer by artificial hemoproteins in carbon nanotubes
64112
Provided by PTCRIS: 64112
PTDC/QUI/64112/2006
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2005/06 - 2008/11 A Murine Neuroblastoma Cell-Based Approach to Study and Prevent Prion Diseases
56668
Provided by PTCRIS: 56668
POCI/CVT/56668/2004
Principal investigator
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2005/10 - 2008/06 Lipid-Lipid and Protein-Lipid Interactions in Model Systems of Cell Membranes
Provided by PTCRIS: 58816
58816
POCI/QUI/58816/2004
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2005/09 - 2008/06 Molecular organization of functionalized porphyrins in giant vesicles and supramolecules
Provided by PTCRIS: 57387
57387
POCI/QUI/57387/2004
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2004/01/15 - 2008 Design of Stabilizing Affinity Molecular Probes : A Rational Approach to Assess and Enhance Protein Stability
info:eu-repo/grantAgreement/FCT/POCI/45296/PT
 Universidade do Algarve Faculdade de Ciências e Tecnologia
Concluded
2003/03/15 - 2006 Relationship between CYP3A4 gene polymorphisms, protein structure and activity in disease setting
info:eu-repo/grantAgreement/FCT/POCI/48417/PT
 Universidade do Algarve Faculdade de Ciências e Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Martins, Lígia O.; Melo, Eduardo Pinho; Sanchez-Amat, Antonio; Robalo, Maria Paula. "Bacterial Laccases: Some Recent Advances and Applications". In Laccases in Bioremediation and Waste Valorisation, 27-55. Springer International Publishing, 2020.
    10.1007/978-3-030-47906-0_2
  2. Melo, Eduardo P. "Estabilidade de Proteínas". In Engenharia Enzimática. LIDEL, 2003.
  3. Ransac, Stéphane; Carrière, Frédéric; Rogalska, Ewa; Verger, Robert; Marguet, Frank; Buono, Gérard; Melo, Eduardo Pinho; et al. "The Kinetics, Specificities and Structural Features of Lipases". In Engineering of/with Lipases, 143-182. Springer Netherlands, 1996.
    10.1007/978-94-009-1671-5_10
  4. Carneiro-Da-Cunha, M. G.; Melo, E. P.; Sebastião, M. J.; Aires-Barros, M. R.; Cabral, J. M. S.. "Separation and Purification of Lipases by Liquid-Liquid Extraction Processes". In Engineering of/with Lipases, 551-576. Springer Netherlands, 1996.
    10.1007/978-94-009-1671-5_35
Conference poster
  1. Botequim, David; Paulo, Pedro M. R.; Prazeres, Duarte; Silva, I. I. R.; Serra, S. G.; Melo, Eduardo P; Costa, Sílvia M.B. "Dye-labeled DNAs Conjugated onto Gold Nanorods for Improved Biosensors". Paper presented in EuroNanoForum 2021, 2021.
Journal article
  1. Gupta, Karnika; Maddison, Daniel; 1437361, user; Rosa da Costa, Ana; Avezov, Edward. "Fluorescence Lifetime-Assisted Probing of Protein Aggregation with sub-Organellar Resolution". BIO-PROTOCOL 14 1354 (2024): https://doi.org/10.21769/bioprotoc.5080.
    Published • 10.21769/bioprotoc.5080
  2. André Taborda; Tomás Frazão; Miguel V. Rodrigues; Xavier Fernández-Luengo; Ferran Sancho; Maria Fátima Lucas; Carlos Frazão; et al. "Mechanistic insights into glycoside 3-oxidases involved in C-glycoside metabolism in soil microorganisms". Nature Communications (2023): https://doi.org/10.1038/s41467-023-42000-3.
    10.1038/s41467-023-42000-3
  3. Melo EP. "Rationally Guided Improvement of NOV1 Dioxygenase for the Conversion of Lignin-Derived Isoeugenol to Vanillin". Biochemistry (2022): http://dx.doi.org/10.1021/acs.biochem.2c00168.
    10.1021/acs.biochem.2c00168
  4. Melo, Eduardo Pinho; Konno, Tasuku; Farace I.; Awadelkareem, M. A.; Skov, L.R.; Teodoro, F.; Sancho, T.P.; et al. Corresponding author: Avezov, Edward. "Stress-induced protein disaggregation in the Endoplasmic Reticulum catalysed by BiP". Nature Commun (2022):
    Open access • In press
  5. Melo EP. "Unveiling molecular details behind improved activity at neutral to alkaline pH of an engineered DyP-type peroxidase". Computational and Structural Biotechnology Journal 20 (2022): 3899-3910. http://dx.doi.org/10.1016/j.csbj.2022.07.032.
    10.1016/j.csbj.2022.07.032
  6. Tasuku Konno; Eduardo Pinho Melo; Joseph E. Chambers; Edward Avezov. "Intracellular Sources of ROS/H2O2 in Health and Neurodegeneration: Spotlight on Endoplasmic Reticulum". Cells 10 2 (2021): 233-233. https://doi.org/10.3390/cells10020233.
    10.3390/cells10020233
  7. Correia, Cátia; Tavares, Evandro; Lopes, Carlos; Silva, Joana G.; Duarte, Andreia; Geraldes, Vitor; Rodrigues, Miguel A.; Melo, Eduardo P.. "Stability of Protein Formulations at Subzero Temperatures by Isochoric Cooling". Journal of Pharmaceutical Sciences 109 1 (2020): 316-322. http://dx.doi.org/10.1016/j.xphs.2019.06.017.
    10.1016/j.xphs.2019.06.017
  8. David Botequim; Inês I. R. Silva; Sofia G. Serra; Eduardo P. Melo; Duarte M. F. Prazeres; Sílvia M. B. Costa; Pedro M. R. Paulo. "Fluorescent dye nano-assemblies by thiol attachment directed to the tips of gold nanorods for effective emission enhancement". Nanoscale 12 (2020): 6334-6345. https://doi.org/10.1039/D0NR00267D.
    Published • 10.1039/D0NR00267D
  9. Pfluck ACD; de Barros DPC; Fonseca LP; Melo EP. "Stability of lipases in miniemulsion systems: Correlation between secondary structure and activity.". Enzyme and microbial technology 114 (2018): 7-14. http://europepmc.org/abstract/med/29685356.
    Published • 10.1016/j.enzmictec.2018.03.003
  10. Macedo JA; Schrama D; Duarte I; Tavares E; Renaut J; Futschik ME; Rodrigues PM; Melo EP. "Membrane-enriched proteome changes and prion protein expression during neural differentiation and in neuroblastoma cells.". 18 (2017): 319. http://europepmc.org/abstract/med/28431525.
    Published • 10.1186/s12864-017-3694-6
  11. Melo EP; Lopes C; Gollwitzer P; Lortz S; Lenzen S; Mehmeti I; Kaminski CF; Ron D; Avezov E. "TriPer, an optical probe tuned to the endoplasmic reticulum tracks changes in luminal H2O2.". (2017): http://europepmc.org/abstract/med/28347335.
    10.1186/s12915-017-0367-5
  12. Anjos L; Morgado I; Guerreiro M; Cardoso JC; Melo EP; Power DM. "Cartilage acidic protein 1, a new member of the beta-propeller protein family with amyloid propensity.". (2017): http://europepmc.org/abstract/med/27862299.
    10.1002/prot.25210
  13. Konno, Tasuku; Pinho Melo, Eduardo; Lopes, Carlos; Mehmeti, Ilir; Lenzen, Sigurd; Ron, David; Avezov, Edward. "ERO1-independent production of H2O2 within the endoplasmic reticulum fuels Prdx4-mediated oxidative protein folding". The Journal of Cell Biology 211 2 (2015): 253-259. http://dx.doi.org/10.1083/jcb.201506123.
    Published • 10.1083/jcb.201506123
  14. Estrela N; Franquelim HG; Lopes C; Tavares E; Macedo JA; Christiansen G; Otzen DE; Melo EP. "Sucrose prevents protein fibrillation through compaction of the tertiary structure but hardly affects the secondary structure.". (2015): http://europepmc.org/abstract/med/26344410.
  15. Avezov E; Konno T; Zyryanova A; Chen W; Laine R; Crespillo-Casado A; Melo EP; et al. "Retarded PDI diffusion and a reductive shift in poise of the calcium depleted endoplasmic reticulum.". (2015): http://europepmc.org/abstract/med/25575667.
    10.1186/s12915-014-0112-2
  16. Tsunoda, Satoshi; Avezov, Edward; Zyryanova, Alisa; Konno, Tasuku; Mendes-Silva, Leonardo; Pinho Melo, Eduardo; Harding, Heather P; Ron, David. "Intact protein folding in the glutathione-depleted endoplasmic reticulum implicates alternative protein thiol reductants". eLife 3 (2014): http://dx.doi.org/10.7554/elife.03421.
    Published • 10.7554/elife.03421
  17. Tavares, Evandro; Macedo, Joana A.; Paulo, Pedro M.R.; Tavares, Catarina; Lopes, Carlos; Melo, Eduardo P.. "Live-cell FRET imaging reveals clustering of the prion protein at the cell surface induced by infectious prions". Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1842 7 (2014): 981-991. http://dx.doi.org/10.1016/j.bbadis.2014.02.002.
    Published • 10.1016/j.bbadis.2014.02.002
  18. Brissos, Vânia; Gonçalves, Nádia; Melo, Eduardo P.; Martins, Lígia O.. "Improving Kinetic or Thermodynamic Stability of an Azoreductase by Directed Evolution". PLoS ONE 9 1 (2014): e87209. http://dx.doi.org/10.1371/journal.pone.0087209.
    Published • 10.1371/journal.pone.0087209
  19. Anjos, Liliana; Gomes, Ana S.; Melo, Eduardo P.; Canário, Adelino V.; Power, Deborah M.. "Cartilage Acidic Protein 2 a hyperthermostable, high affinity calcium-binding protein". Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1834 3 (2013): 642-650. http://dx.doi.org/10.1016/j.bbapap.2012.12.012.
    Published • 10.1016/j.bbapap.2012.12.012
  20. Avezov, E.; Cross, B.C.S.; Schierle, G.S.K.; Winters, M.; Harding, H.P.; Melo, E.P.; Kaminski, C.F.; Ron, D.. "Lifetime imaging of a fluorescent protein sensor reveals surprising stability of ER thiol redox". Journal of Cell Biology 201 2 (2013): 337-349. http://www.scopus.com/inward/record.url?eid=2-s2.0-84876716167&partnerID=MN8TOARS.
    Published • 10.1083/jcb.201211155
  21. Rosa, M.; Lopes, C.; Melo, E.P.; Singh, S.K.; Geraldes, V.; Rodrigues, M.A.. "Measuring and modeling hemoglobin aggregation below the freezing temperature". Journal of Physical Chemistry B 117 30 (2013): 8939-8946. http://www.scopus.com/inward/record.url?eid=2-s2.0-84881244021&partnerID=MN8TOARS.
    Published • 10.1021/jp4035369
  22. Fernandes, A.T.; Lopes, C.; Martins, L.O.; Melo, E.P.. "Unfolding pathway of CotA-laccase and the role of copper on the prevention of refolding through aggregation of the unfolded state". Biochemical and Biophysical Research Communications 422 3 (2012): 442-446. http://www.scopus.com/inward/record.url?eid=2-s2.0-84861938261&partnerID=MN8TOARS.
    Published • 10.1016/j.bbrc.2012.05.011
  23. Fernandes, A.T.; Pereira, M.M.; Silva, C.S.; Lindley, P.F.; Bento, I.; Melo, E.P.; Martins, L.O.. "The removal of a disulfide bridge in CotA-laccase changes the slower motion dynamics involved in copper binding but has no effect on the thermodynamic stability". Journal of Biological Inorganic Chemistry 16 4 (2011): 641-651. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955753026&partnerID=MN8TOARS.
    Published • 10.1007/s00775-011-0768-9
  24. Zito, E.; Melo, E.P.; Yang, Y.; Wahlander, Å.; Neubert, T.A.; Ron, D.. "Oxidative Protein Folding by an Endoplasmic Reticulum-Localized Peroxiredoxin". Molecular Cell 40 5 (2010): 787-797. http://www.scopus.com/inward/record.url?eid=2-s2.0-78649918283&partnerID=MN8TOARS.
    Published • 10.1016/j.molcel.2010.11.010
  25. Melo, E.P.; Estrela, N.; Lopes, C.; Matias, A.C.; Tavares, E.; Ochoa-Mendes, V.. "Compacting proteins: Pros and cons of osmolyte-induced folding". Current Protein and Peptide Science 11 8 (2010): 744-751. http://www.scopus.com/inward/record.url?eid=2-s2.0-79951728885&partnerID=MN8TOARS.
    Published • 10.2174/138920310794557727
  26. Fernandes, A.T.; Martins, L.O.; Melo, E.P.. "The hyperthermophilic nature of the metallo-oxidase from Aquifex aeolicus". Biochimica et Biophysica Acta - Proteins and Proteomics 1794 1 (2009): 75-83. http://www.scopus.com/inward/record.url?eid=2-s2.0-56449087972&partnerID=MN8TOARS.
    Published • 10.1016/j.bbapap.2008.09.006
  27. Madeira, C.; Estrela, N.; Ferreira, J.A.B.; Andrade, S.M.; Costa, S.M.B.; Melo, E.P.. "Fluorescence lifetime imaging microscopy and fluorescence resonance energy transfer from cyan to yellow fluorescent protein validates a novel method to cluster proteins on solid surfaces". Journal of Biomedical Optics 14 4 (2009): http://www.scopus.com/inward/record.url?eid=2-s2.0-73349116900&partnerID=MN8TOARS.
    Published • 10.1117/1.3210770
  28. Durão, P.; Chen, Z.; Fernandes, A.T.; Hildebrandt, P.; Murgida, D.H.; Todorovic, S.; Pereira, M.M.; Melo, E.P.; Martins, L.O.. "Copper incorporation into recombinant CotA laccase from Bacillus subtilis: Characterization of fully copper loaded enzymes". Journal of Biological Inorganic Chemistry 13 2 (2008): 183-193. http://www.scopus.com/inward/record.url?eid=2-s2.0-38649103815&partnerID=MN8TOARS.
    Published • 10.1007/s00775-007-0312-0
  29. Baptista, R.P.; Pedersen, S.; Cabrita, G.J.M.; Otzen, D.E.; Cabral, J.M.S.; Melo, E.P.. "Thermodynamics and mechanism of cutinase stabilization by trehalose". Biopolymers 89 6 (2008): 538-547. http://www.scopus.com/inward/record.url?eid=2-s2.0-44849111668&partnerID=MN8TOARS.
    Published • 10.1002/bip.20926
  30. Brissos, V.; Melo, E.P.; Martinho, J.M.G.; Cabral, J.M.S.. "Biochemical and structural characterisation of cutinase mutants in the presence of the anionic surfactant AOT". Biochimica et Biophysica Acta - Proteins and Proteomics 1784 9 (2008): 1326-1334. http://www.scopus.com/inward/record.url?eid=2-s2.0-53149145988&partnerID=MN8TOARS.
    Published • 10.1016/j.bbapap.2008.04.017
  31. Morgado, I.; Melo, E.P.; Lundberg, E.; Estrela, N.L.; Sauer-Eriksson, A.E.; Power, D.M.. "Hormone affinity and fibril formation of piscine transthyretin: The role of the N-terminal". Molecular and Cellular Endocrinology 295 1-2 (2008): 48-58. http://www.scopus.com/inward/record.url?eid=2-s2.0-54949157374&partnerID=MN8TOARS.
    Published • 10.1016/j.mce.2008.06.010
  32. Neves-Petersen, Maria Teresa; Klitgaard, Søren; Carvalho, Ana Sofia Leitão; Petersen, Steffen B.; Aires de Barros, Maria Raquel; e Melo, Eduardo Pinho. "Photophysics and Photochemistry of Horseradish Peroxidase A2 upon Ultraviolet Illumination". Biophysical Journal 92 6 (2007): 2016-2027. http://dx.doi.org/10.1529/biophysj.106.095455.
    Published • 10.1529/biophysj.106.095455
  33. Chen, Z.; Franco, C.F.; Baptista, R.P.; Cabral, J.M.S.; Coelho, A.V.; Rodrigues Jr., C.J.; Melo, E.P.. "Purification and identification of cutinases from Colletotrichum kahawae and Colletotrichum gloeosporioides". Applied Microbiology and Biotechnology 73 6 (2007): 1306-1313. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846176102&partnerID=MN8TOARS.
    Published • 10.1007/s00253-006-0605-1
  34. Otzen, D.E.; Giehm, L.; Baptista, R.P.; Kristensen, S.R.; Melo, E.P.; Pedersen, S.. "Aggregation as the basis for complex behaviour of cutinase in different denaturants". Biochimica et Biophysica Acta - Proteins and Proteomics 1774 2 (2007): 323-333. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846795794&partnerID=MN8TOARS.
    Published • 10.1016/j.bbapap.2006.11.012
  35. Carvalho, A.S.L.; Ferreira, B.S.; Neves-Petersen, M.T.; Petersen, S.B.; Aires-Barros, M.R.; Melo, E.P.. "Thermal denaturation of HRPA2: pH-dependent conformational changes". Enzyme and Microbial Technology 40 4 (2007): 696-703. http://www.scopus.com/inward/record.url?eid=2-s2.0-33847263892&partnerID=MN8TOARS.
    Published • 10.1016/j.enzmictec.2006.05.029
  36. Viseu, M.I.; Melo, E.P.; Carvalho, T.I.; Correia, R.F.; Costa, S.M.B.. "Unfolding kinetics of ß-lactoglobulin induced by surfactant and denaturant: A stopped-flow/fluorescence study". Biophysical Journal 93 10 (2007): 3601-3612. http://www.scopus.com/inward/record.url?eid=2-s2.0-36549015809&partnerID=MN8TOARS.
    Published • 10.1529/biophysj.106.101667
  37. Melo, E.P.; Fernandes, A.T.; Durão, P.; Martins, L.O.. "Insight into stability of CotA laccase from the spore coat of Bacillus subtilis". Biochemical Society Transactions 35 6 (2007): 1579-1582. http://www.scopus.com/inward/record.url?eid=2-s2.0-37749011277&partnerID=MN8TOARS.
    Published • 10.1042/BST0351579
  38. Chen, L.; Ferreira, J.A.B.; Costa, S.M.B.; Cabrita, G.J.M.; Otzen, D.E.; Melo, E.P.. "Compaction of ribosomal protein S6 by sucrose occurs only under native conditions". Biochemistry 45 7 (2006): 2189-2199. http://www.scopus.com/inward/record.url?eid=2-s2.0-33144477068&partnerID=MN8TOARS.
    Published • 10.1021/bi051490g
  39. Durão, P.; Bento, I.; Fernandes, A.T.; Melo, E.P.; Lindley, P.F.; Martins, L.O.. "Perturbations of the T1 copper site in the CotA laccase from Bacillus subtilis: Structural, biochemical, enzymatic and stability studies". Journal of Biological Inorganic Chemistry 11 4 (2006): 514-526. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745747078&partnerID=MN8TOARS.
    Published • 10.1007/s00775-006-0102-0
  40. Pedersen, S.; Nesgaard, L.; Baptista, R.P.; Melo, E.P.; Kristensen, S.R.; Otzen, D.E.. "pH-dependent aggregation of cutinase is efficiently suppressed by 1,8-ANS". Biopolymers 83 6 (2006): 619-629. http://www.scopus.com/inward/record.url?eid=2-s2.0-33845596138&partnerID=MN8TOARS.
    Published • 10.1002/bip.20598
  41. Rodrigues, M.L.; Archer, M.; Martel, P.; Miranda, S.; Thomaz, M.; Enguita, F.J.; Baptista, R.P.; et al. "Crystal structures of the free and sterol-bound forms of ß-cinnamomin". Biochimica et Biophysica Acta - Proteins and Proteomics 1764 1 (2006): 110-121. http://www.scopus.com/inward/record.url?eid=2-s2.0-33644834490&partnerID=MN8TOARS.
    Published • 10.1016/j.bbapap.2005.09.008
  42. Carvalho, A.S.L.; Neves-Petersen, M.T.; Petersen, S.B.; Aires-Barros, M.R.; Pinho E Melo, E.. "Formation of a misfolded conformation during refolding of HRPA1 in the presence of calcium". Biochimica et Biophysica Acta - Proteins and Proteomics 1747 1 (2005): 99-107. http://www.scopus.com/inward/record.url?eid=2-s2.0-12844249413&partnerID=MN8TOARS.
    Published • 10.1016/j.bbapap.2004.10.002
  43. Chen, L.; Cabrita, G.J.M.; Otzen, D.E.; Melo, E.P.. "Stabilization of the ribosomal protein S6 by trehalose is counterbalanced by the formation of a putative off-pathway species". Journal of Molecular Biology 351 2 (2005): 402-416. http://www.scopus.com/inward/record.url?eid=2-s2.0-22444433527&partnerID=MN8TOARS.
    Published • 10.1016/j.jmb.2005.05.056
  44. Carvalho, A.S.L.; Santos, A.M.; Neves-Petersen, M.T.; Petersen, S.B.; Aires-Barros, M.R.; Pinho E Melo, E.. "Conformational states of HRPA1 induced by thermal unfolding: Effect of low molecular weight solutes". Biopolymers 75 2 (2004): 173-186. http://www.scopus.com/inward/record.url?eid=2-s2.0-4944228100&partnerID=MN8TOARS.
    Published • 10.1002/bip.20100
  45. Melo, E.P.; Chen, L.Y.; Cabral, J.M.S.; Fojan, P.; Petersen, S.B.; Otzen, D.E.. "Trehalose favors a cutinase compact intermediate off-folding pathway". Biochemistry 42 24 (2003): 7611-7617. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038070096&partnerID=MN8TOARS.
    Published • 10.1021/bi034267x
  46. Baptista, R.P.; Chen, L.Y.; Paixão, A.; Cabral, J.M.S.; Melo, E.P.. "A novel pathway to enzyme deactivation: The cutinase model". Biotechnology and Bioengineering 82 7 (2003): 851-857. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038247895&partnerID=MN8TOARS.
    Published • 10.1002/bit.10641
  47. Melo, E.P.; Baptista, R.P.; Cabral, J.M.S.. "Improving cutinase stability in aqueous solution and in reverse micelles by media engineering". Journal of Molecular Catalysis B: Enzymatic 22 5-6 (2003): 299-306. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038692188&partnerID=MN8TOARS.
    Published • 10.1016/S1381-1177(03)00044-4
  48. Carvalho, A.S.L.; Melo, E.P.E.; Ferreira, B.S.; Neves-Petersen, M.T.; Petersen, S.B.; Aires-Barros, M.R.. "Heme and pH-dependent stability of an anionic horseradish peroxidase". Archives of Biochemistry and Biophysics 415 2 (2003): 257-267. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038385478&partnerID=MN8TOARS.
    Published • 10.1016/S0003-9861(03)00275-3
  49. Melo, E.P.; Costa, S.M.B.; Cabral, J.M.S.; Fojan, P.; Petersen, S.B.. "Cutinase - AOT interactions in reverse micelles: The effect of 1-hexanol". Chemistry and Physics of Lipids 124 1 (2003): 37-47. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037685261&partnerID=MN8TOARS.
    Published • 10.1016/S0009-3084(03)00031-8
  50. Melo, E.P.; Faria, T.Q.; Martins, L.O.; Gonalves, A.M.; Cabral, J.M.S.. "Cutinase unfolding and stabilization by trehalose and mannosylglycerate". Proteins: Structure, Function and Genetics 42 4 (2001): 542-552. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035282963&partnerID=MN8TOARS.
    Published • 10.1002/1097-0134(20010301)42:4<542::AID-PROT120>3.0.CO;2-4
  51. Melo, E.P.; Aires-Barros, M.R.; Cabral, J.M.S.. "Reverse micelles and protein biotechnology". Biotechnology Annual Review 7 (2001): 87-129. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035237050&partnerID=MN8TOARS.
    Published • 10.1016/S1387-2656(01)07034-X
  52. Melo, E.P.; Fojan, P.; Cabral, J.M.S.; Petersen, S.B.. "Dynamic light scattering of cutinase in AOT reverse micelles". Chemistry and Physics of Lipids 106 2 (2000): 181-189. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033897324&partnerID=MN8TOARS.
    Published • 10.1016/S0009-3084(00)00152-3
  53. Melo, E.P.; Taipa, M.A.; Castellar, M.R.; Costa, S.M.B.; Cabral, J.M.S.. "A spectroscopic analysis of thermal stability of the Chromobacterium viscosum lipase". Biophysical Chemistry 87 2-3 (2000): 111-120. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034735624&partnerID=MN8TOARS.
    Published • 10.1016/S0301-4622(00)00171-X
  54. Baptista, R.P.; Cabral, J.M.S.; Melo, E.P.. "Trehalose delays the reversible but not the irreversible thermal denaturation of cutinase". Biotechnology and Bioengineering 70 6 (2000): 699-703. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034694819&partnerID=MN8TOARS.
    Published • 10.1002/1097-0290(20001220)70:6<699::AID-BIT13>3.0.CO;2-N
  55. Carvalho, C.M.L.; Melo, E.P.; Costa, S.M.B.; Cabral, J.M.S.; Aires-Barros, M.R.. "A steady-state fluorescence study of cutinase microencapsulated in AOT reversed micelles at optimal stability conditions". Biotechnology Letters 21 8 (1999): 673-681. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032830485&partnerID=MN8TOARS.
    Published • 10.1023/A:1005550213632
  56. Melo, E.P.; Carvalho, C.M.L.; Aires-Barros, M.R.; Costa, S.M.B.; Cabral, J.M.S.. "Deactivation and conformational changes of cutinase in reverse micelles". Biotechnology and Bioengineering 58 4 (1998): 380-386. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032550831&partnerID=MN8TOARS.
    Published • 10.1002/(SICI)1097-0290(19980520)58:4<380::AID-BIT5>3.0.CO;2-F
  57. Pinho Melo, E.; Aires-Barros, M.R.; Costa, S.M.B.; Cabral, J.M.S.. "Thermal unfolding of proteins at high pH range studied by UV absorbance". Journal of Biochemical and Biophysical Methods 34 1 (1997): 45-59. http://dx.doi.org/10.1016/s0165-022x(97)01202-5.
    Published • 10.1016/s0165-022x(97)01202-5
  58. Carneiro-da-Cunha, M.G.; Melo, E.P.; Cabral, J.M.S.; Aires-Barros, M.R.. "Liquid-liquid extraction of a recombinant cutinase from fermentation media with AOT reversed micelles". Bioprocess Engineering 15 3 (1996): 151-157. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029763705&partnerID=MN8TOARS.
    Published • 10.1007/s004490050249
  59. Melo, E.P.; Costa, S.M.B.; Cabral, J.M.S.. "Denaturation of a recombinant cutinase from Fusarium solani in AOT-iso-octane reverse micelles: A steady-state fluorescence study". Photochemistry and Photobiology 63 2 (1996): 169-175. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030038712&partnerID=MN8TOARS.
    Published
  60. Melo, E. Pinho; Ivanova, Margarita G.; Aires-Barros, M. Raquel; Cabral, Joaquim M. S.; Verger, Robert. "Glyceride synthesis catalyzed by cutinase using the monomolecular film technique". Biochemistry 34 5 (1995): 1615-1621. http://dx.doi.org/10.1021/bi00005a018.
    Published • 10.1021/bi00005a018
  61. Melo, E. P.; Airesbarros, M. R.; Cabral, J. M. S.. "Triglyceride hydrolysis and stability of a recombinant cutinase fromFusarium solani in AOT-iso-octane reversed micelles". Applied Biochemistry and Biotechnology 50 1 (1995): 45-56. http://dx.doi.org/10.1007/bf02788039.
    Published • 10.1007/bf02788039
  62. Melo, E.P.; Costa, S.M.B.; Cabral, J.M.S.. "Structural effect of reversed micelles of AOT over a recombinant cutinase from Fusarium solani pisi - A steady state fluorescence study". Annals of the New York Academy of Sciences 750 (1995): 85-88. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029129510&partnerID=MN8TOARS.
    Published • 10.1111/j.1749-6632.1995.tb19931.x
  63. Setti, L.; Fevereiro, P.; Melo, E.P.; Pifferi, P.G.; Cabral, J.M.S.; Aires-Barros, M.R.. "Superactivity of peroxidase solubilized in reversed micellar systems". Applied Biochemistry and Biotechnology 55 3 (1995): 207-218. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029436379&partnerID=MN8TOARS.
    Published • 10.1007/BF02786860
  64. Barreto, M. T. O.; Melo, E. P.; Almeida, J. S.; Xavier, A. M. R. B.; Carrondo, M. J. T.. "A kinetic method for calculating the viability of lactic starter cultures". Applied Microbiology and Biotechnology 34 5 (1991): 648-652. http://dx.doi.org/10.1007/bf00167916.
    Published • 10.1007/bf00167916
  65. Barreto, M. T. O.; Melo, E. P.; Moreira, J. L.; Carrondo, M. J. T.. "High cell density reactor for the production of Lactobacillus plantarum". Journal of Industrial Microbiology 7 1 (1991): 63-69. http://dx.doi.org/10.1007/bf01575605.
    Published • 10.1007/bf01575605
  66. Barreto, M. T. O.; Melo, E. P.; Carrondo, M. J. T.. "Starter culture production in fluidized bed reactor with a flocculent strain of L. plantarum". Biotechnology Letters 11 5 (1989): 337-342. http://dx.doi.org/10.1007/bf01024514.
    Published • 10.1007/bf01024514
Preprint
  1. Eduardo P. Melo; Soukaina El-Guendouz; Cátia Correia; Fernando Teodoro; Carlos Lopes; Paulo J Martel. "A conformational-dependent interdomain redox relay at the core of Protein Disulfide Isomerase activity". 2023. https://doi.org/10.1101/2023.02.24.529848.
    10.1101/2023.02.24.529848

Intellectual property

Patent
  1. Melo, Eduardo P. 2014. "PROCESSO E APARELHO DE CONGELAÇÃO EM PRATOS PARA OBTENÇÃO DE MATRIZES DE GELO COM DISTRIBUIÇÃO DE SOLUTOS UNIFORME". Portugal.
    Granted/Issued

Other

Other output
  1. TriPer, an optical probe tuned to the endoplasmic reticulum tracks H2O2 consumption by glutathione. 2016. Eduardo Pinho Melo; Carlos Lopes; Peter Gollwitzer; Stephan Lortz; Sigurd Lenzen; Ilir Mehmeti; Clemens F. Kaminski; David Ron; Edward Avezov. https://doi.org/10.1101/088351.
    10.1101/088351
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/09/06 A conformational-dependent intramolecular redox relay at the core of Protein Disulfide Isomerase activity Discover Molecular Interactions, User meeting_Dynamic Biosensors
(Munich, Germany)
2021 Expression of the prion protein and membrane-enriched proteome changes during neural differentiation and in neuroblastoma cells 7th International Caparica Conference on Analytical Proteomics
Universidade Nova de Lisboa (Costa da Caparica)
2019 Stability of protein formulations at sub-zero temperatures by isochoric cooling Biological and pharmaceutical complex fluids III: Protein self-assembly, rheology and interfacial properties
(Hernstein, Austria)
2018 Live-cell imaging of protein disaggregation in the endoplasmic reticulum 7th Iberian congress on prions
(Laguardia, Spain)
2016 The Yin and Yang of Protein Aggregation ProtStab 2016, 11th International Conference on Protein Stabilisation
(Istanbul, Turkey)
2015 Getting proteins more compact prevents protein amyloidosis 4th Iberian Congress on Prions
(Lisboa, Portugal)
2015 Membrane-enriched proteomic changes associated with prion protein expression during neural differentiation and in neuroblastoma cells 4th Iberian Congress on Prions
(Lisboa, Portugal)
2014 Membrane proteome and prion protein profiling during neural differentiation of stem cells 3rd Iberian Congress on Prions
(Zaragoza, Spain)
2014 Osmolyte induce compaction of the protein tertiary structure preventing amyloid fiber formation and protein aggregation below the freezing temperature 10th International Conference on Protein Stabilization
(Stresa, Italy)
2014 Accelerated Formulation Studies for Frozen Storage of Proteins 9th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology
(Lisboa, Portugal)
2013 Live-Cell imaging FRET reveals clustering of the prion protein at the cell surface induced by infectious prions 2nd Iberian Congress on Prions
(Faro, Portugal)
2013 Stabilization of FMN-dependent NADPH:dye/quinone reductase from Pseudomonas putida MET94 by directed evolution 9th European Biophysical Congress
(Lisboa, Portugal)
2012 Detection and Neurotoxicity of Prions 1st Iberian Congress on Prions
(Santiago de Compostela, Spain)
2012 Protein fibrillation, osmolytes and prion diseases 9th International Conference on Protein Stabilization
(Lisboa, Portugal)
2011 Protein Fibrillation, Prion Diseases and the Origin of Life I Ciclo de Conferências – Núcleo de Estudantes de Ciências Biomédicas
(Faro, Portugal)
2010 Stability of CotA – laccase from Bacillus subtilis and Metallo-Oxidase McoA from Aquifex aeolicus to thermal and chemical stability Oxidative Enzymes as Sustainable Industrial Biocatalysts
(Santiago de Compostela, Spain)
2010 Insight into the stability of multicopper oxidases Protein-Protein Interactions and Protein folding
Universidade Nova de Lisboa (Lisboa, Portugal)
2010 Insight into the stability of multicopper oxidases Journées Jeunes Chercheurs
(Bourdeaux, France)
2010 Insight into the stability of multicopper oxidases Oxizymes & 9th International Symposium of Peroxidases
(Leipzig, Germany)
2010 Insight into the stability of multicopper oxidases 3rd Annual World Congress of Industrial Biotechnology – IBIO 2010
(Dalian, China)
2009 Protein Fibrillation is prevented by Osmolytes 8th International Conference on Protein Stabilization
(Graz, Austria)
2008 Folding and amyloidosis of proteins: The prevention of amyloidosis by osmolytes XVIth National Congress of Biochemistry
(Ponta Delgada, Portugal)
2008 Fluorescence studies of the pair CFP-YFP: FLIM-FRET in vitro studies and expression in neuronal cells to probe the prion protein at the cell surface Focus on Microscopy
(Osaka, Japan)
2008 Copper incorporation into recombinant CotA-laccase from Bacillus subtilis Oxizymes, 4th European meeting in Oxizymes
(Helsinki, Finland)
2007 Insight Into the Stability of Multi-Copper Oxidases 7th International Conference on Protein Stabilization
(Exeter, United Kingdom)
2006 Priões Forum da Química
Universidade Nova de Lisboa (Lisboa, Portugal)
2006 Enzyme Stabilization: different strategies for different enzymes 6th European Symposiun on Biochemical Engineering Science
(Salzburg, Austria)
2006 Perturbations of the T1 copper site in the CotA-laccase from Bacillus subtilis: Structural, Biochemical, Enzymatic and Stability Studies Eurobic8. 8th European Biological Inorganic Chemistry Conference
(Aveiro, Portugal)
2006 Enzymatic properties, structural insights and stability of a highly efficient metallo-oxidase from the hyperthermophilic bacterium Aquifex aeolicus Copper 06. 5th International Copper Meeting
(Alghero, Italy)
2005 Protein Folding and Stabilization by Osmolytes International Conference on Enzyme Technology, RELATENZ 2005
(Varadero, Cuba)
2004 Effect of Trehalose on Folding and Stability of the Ribosomal Protein S6 6th International Conference on Protein Stabilization
(Bratislava, Slovakia)
2001 Cutinase Folding and Stabilization by Trehalose 10th European Congress on Biotechnology
(Madrid, Spain)
1997 Deactivation Equilibria for Cutinase in Reverse Micelles 8th European Congress on Biotechnology
(Budapeste, Hungary)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2021 - 2021 In vitro protein thiol oxidation by endoplasmic reticulum enzymatic pathways
Supervisor
 Biological Engineering (Master)
Universidade do Algarve, Portugal
2012 - 2016 Development of Technology for cryopreservation and formulation of therapeutic proteins
Co-supervisor
 Chemical Engineering (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2013 - 2013 Catálise de pontes dissulfureto pelas vias enzimáticas que actuam no retículo endoplasmático usando como substrato a proteína fluorescente HyPer
Supervisor
 Biological Engineering (Master)
Universidade do Algarve, Portugal
2013 - 2013 Neurotoxicidade de fibrilhas da proteína priónica
Supervisor
 Biological Engineering (Master)
Universidade do Algarve, Portugal
2012 - 2012 Fibrillation and neurotoxicity of the prion protein
Supervisor
 Biological Engineering (Master)
Universidade do Algarve - Campus de Gambelas, Portugal
2012 - 2012 Oxidative protein folding in the endoplasmic reticulum
Supervisor
 Biological Engineering (Master)
Universidade do Algarve, Portugal
2005 - 2010 Prion-Diseases: A Biophysical approach to the pathological protein conformer
Supervisor
 Biological Sciences (PhD)
Universidade do Algarve, Portugal
2009 - 2009 Prião Murinico: Produção, separação, mutagenese e estabilidade à autólise
Supervisor
 Biological Engineering (Master)
Universidade do Algarve, Portugal
2005 - 2009 Structure-Function relationships and stability of extremophilic lacases
Co-supervisor
 Biochemistry (PhD)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2003 - 2007 Protein Stabilization by media engineering and directed evolution
Supervisor
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2001 - 2005 Folding and Stability of Proteins in the presence of Low Molecular Weight Solutes
Supervisor of Chen Luyang
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2000 - 2004 Conformational Stability Studies of Two Anionic Horseradish Peroxidases Isoenzymes
Co-supervisor
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2001 - 2001 Improving thermal/structural stability of cutinase in the presence of low molecular weight solutes and by directed evolution
Supervisor
 Biotechnology (Master)
Aalborg Universitet Institut for Kemi og Biovidenskab, Denmark

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2018 - 2018 Organizing Committee Member of Prion 2018 (2018 - 2018)
Conference (Member of the Organising Committee)
 Universidade de Santiago de Compostela, Spain
2013 - 2013 Head of the organizing committee (2013 - 2013)
Conference (President of the Organising Committee)
 Universidade do Algarve, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2013 - 2013 Chairman of the “Protein Folding” session
Congress
 9th European Biophysical Congress
2012 - 2012 Chairman at the 9th International Conference on Protein Stabilisation 9th International Conference on Protein Stabilisation
2005 - 2005 Chairman at the International Conference on Enzyme Technology, RELATENZ 2005
Congress
 International Conference on Enzyme Technology, RELATENZ 2005
2004 - 2004 Chairman of the 6th International Conference on Protein Stabilization
Congress
 6th International Conference on Protein Stabilization
2000 - 2000 Chairman of the Protein Folding session
Congress
 2nd International Conference on Protein Stabilization

Conference scientific committee

Conference name Conference host
2020 - 2020 9th Iberian Congress on Prions Corunha, Espanha
2018 - 2018 7th Iberian Congress on Prions Laguardia, Espanha
2016 - 2016 5th Iberian Congress on Prions Barcelona, Espanha
2015 - 2015 4th Iberian Congress on Prions Lisboa, Portugal
2012 - 2012 1st Iberian Congress on Prions Santiago de Compostela, Espanha

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2011 - 2011 Global approach to proteomics (Especialização pós-licenciatura) Universidade do Algarve, Portugal
2010 - 2010 Folding kinetics and Stability Protein folding, misfolding and disease, 9th short course of the Portuguese Biophysical Society (Especialização pós-licenciatura)
2007 - 2007 Proteins at the Cell Surface Probed by Fluorescence Lifetime Imaging Microscopy Portugal-MIT program on Bioengineering (Especialização pós-licenciatura) Universidade de Lisboa Instituto Superior Técnico, Portugal
1999 - 1999 Lipase interactions with Micelles Structure, Function and Stability of Lipases (Doktor (PhD)) Aalborg Universitet, Denmark