My undergraduate background is Marine Biology and I have a masters degree in Molecular and Cellular biology. I have large experience in zebrafish manipulation, such as transgenesis assays for enhancer activity, genotyping techniques, microinjection in one-cell stage, immunohistochemistry, confocal imaging, cryopreservation, CRISPR-Cas9 editing and screening in fluorescence microscopy, including in vivo caracterization of disease models, such as Type 2 Diabetes, in zebrafish. Furthermore, having the course FELASA functions A, B and D of the European Directive 2010/63 , I have all the necessary training to handle, manipulate and design research projects using mouse, rat, zebrafish and fish.. Additionally, I am highly experienced in cell culture techniques, including lentiviral infection, luciferase and loss-of-function assays. I held a research fellow (ERC201805) under the project The Pancreas Regulome: From causality to prediction of non-coding mutations in human pancreatic diseases, where I acquired and improved my skills in reporter assays in vivo in zebrafish and in vitro, in cell lines, using CRISPR based techniques. Also, I learned bioinformatic tools to explore data regarding cis-regulatory elements. The knowledge of transcriptional and cis-regulation acquired in the developing of my master project allowed me to have a special interest in how the cis-regulatory elements can work in perfect timepoints and locations. Recently, I had two first-author publications, including an article in the Diabetes American Journal. I have completed my PhD, supported by a FCT PhD fellowship (SFRH/BD/147762/2019), where I investigated the impact of cis-regulatory disruption on the development of type 2 diabetes. Currently, I have joined the Synapse Biology Group to investigate local translation within Müller glia in the retina. My research aims to understand how local translation affects these glial cells, which could provide valuable insights into their roles in synaptic regulation
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Personal identification

Full name
Ana Eufrásio

Citation names

  • Eufrásio, Ana

Author identifiers

Ciência ID
6514-65E1-6C69
ORCID iD
0000-0002-2753-7874

Email addresses

  • ana.eufrasio@i3s.up.pt (Professional)

Telephones

Mobile phone
  • (+351) 912292329 (Personal)

Addresses

  • Rua Sol Vida, nº11, 1º direito Poente, 4400-593, Canidelo, Vila Nova de Gaia, Portugal (Personal)

Knowledge fields

  • Natural sciences - Biological Sciences - Biology

Languages

Language Speaking Reading Writing Listening Peer-review
English Upper intermediate (B2) Advanced (C1) Intermediate (B1) Upper intermediate (B2) Upper intermediate (B2)
Portuguese (Mother tongue)
Spanish; Castilian Elementary (A2) Elementary (A2) Elementary (A2) Elementary (A2) Elementary (A2)
Degree Classification
2020/01 - 2024/07/22
Concluded
 Biologia Molecular e Celular (Doutoramento)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2023/05/08 - 2023/05/19
Concluded
 Laboratory Animal Science Course - Functions A, B and D (Outros)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2023 - 2023
Concluded
 PhD ladder program (Outros)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2023 - 2023
Concluded
 Introduction to Python and Machine Learning for the Biosciences - 6th Edition (Outros)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022/11/21 - 2022/11/25
Concluded
 Advanced Course on Organoids Models (Outros)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022/05/23 - 2022/05/27
Concluded
 High Throughput Screening and Image Analysis for BioSciences, 6th edition (Outros)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019/12/12 - 2019/12/12
Concluded
 Practical introduction to zebrafish sperm freezing and in vitro fertilization methods (Outros)
Karlsruher Institut für Technologie Institut für Toxikologie und Genetik, Germany
2016/09/12 - 2018/12/20
Concluded
 Biologia Molecular e Celular (Mestrado)
Universidade de Aveiro, Portugal
"Testing the cis-regulatory potential of type 2 diabetes associated non-coding sequences" (THESIS/DISSERTATION)
 18
2018 - 2018
Concluded
 Fish introductory course – Zebrafish and seabass – FELASA B (Outros)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2016 - 2016
Concluded
 Using zebrafish as a model in biomedicine and study of human diseases (Outros)
Centro de Ciências do Mar, Portugal
2012/09/10 - 2015/07/24
Concluded
 Biologia Marinha (Licenciatura)
Universidade do Algarve - Campus de Gambelas, Portugal
"Resposta ao stress em peixes antárticos sujeitos a alterações climáticas" (THESIS/DISSERTATION)
 14

Science

Category
Host institution 		Employer
2024/08/01 - Current Postdoc (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Others

Category
Host institution 		Employer
2020/01/01 - 2024/07/22 PhD scholarship (SFRH/BD/147762/2019) Fundação para a Ciência e a Tecnologia, Portugal
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2018/11/01 - 2019/09/01 Fellowship (ERC201805-ZPR-BIL) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Grant

Designation Funders
2018/11/01 - 2019/09/01 The Pancreas Regulome: From causality to prediction of non-coding mutations in human pancreatic diseases
info:eu-repo/grantAgreement/EC/H2020/680156/EU
Master Student Fellow
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 European Commission
Ongoing

Contract

Designation Funders
2019/01/01 - 2019/12/31 Institute for Research and Innovation in Health Sciences
UID/BIM/04293/2019
LA/P/0070/2020
10.54499/LA/P/0070/2020
Instituto Nacional de Engenharia Biomédica, Portugal

Universidade do Porto, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Other

Designation Funders
2015/11/01 - 2016/08/31 ZEBRAFEEDS
23000
Other
Centro de Ciências do Mar, Portugal

Sparos Lda, Portugal
Fundo Europeu de Desenvolvimento Regional
Concluded
2015/11/01 - 2016/08/31 Cryopreservation of zebrafish germ cells: technological improvements and methodological standardization for gene banking and management
SFRH/BD/97466/2013
Other
Centro de Ciências do Mar, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing

Publications

Conference abstract
  1. Eufrásio, Ana; Bessa, José. "Genetic variants associated to type 2 diabetes modulate endocrine coding enhancers in vivo". Paper presented in 15th International Symposium on Variants in the Genome, Porto, 2024.
  2. Eufrásio, Ana; Jose, Jovel Varghese; José Bessa. "Genetic variants associated to type 2 diabetes modulate endocrine coding enhancers in vivo". Paper presented in 6th Phday - Brilliant PhDs, brighter future: Shaping Science together!, Porto, 2023.
  3. A.Eufrásio; C.Perrod; F.J. Ferreira; M. Duque; M. Galhardo; J. Bessa. "Genetic variants associated to type 2 diabetes modulate endocrine enhancers in vivo". Paper presented in 8th MCBiology Symposium, Porto, 2022.
  4. Bordeira-Carriço, R.; Teixeira, J.; Duque, M.; Ribeiro, D.; Galhardo, M; Dominguez-Acemel, Rafael; Firbas,Panos; et al. "Cis-regulatory similarities in the zebrafish and human pancreas uncover potential disease-related enhancers". Paper presented in 7th Molecular and Cell Biology Virtual Symposium | Science Must Go On, 2021.
  5. Eufrásio, Ana; Perrod, C. AE13-ACCD-75F7; Ferreira, F. J.; Duque, M.; Galhardo, M.; Bessa, J.. "Genetic variants associated to type 2 diabetes modulate endocrine enhancers in vivo". Paper presented in 11th European Zebrafish Meeting 2020, 2020.
  6. Bordeira-Carriço, R; Teixeira, J.; Duque, M.; Ribeiro, D.; Galhardo, M.; Dominguez-Acemel, R; Firbas,Panos; et al. "Cis-regulatory similarities in the zebrafish and human pancreas uncover potential disease-related enhancers". Paper presented in Virtual EMBL Conference: Transcription and Chromatin, 2020.
  7. Eufrásio, Ana; José Bessa. "Testing the cis-regulatory potential of diabetes associated non-coding sequences". Paper presented in Special DiA meeting Algarve - Sociedade Portuguesa de Biologia do Desenvolvimento, 2018.
  8. Diogo, Patrícia; Gil Martins; Eufrásio, Ana; Rita Nogueira; Isa Quinzico; Silva, Tomé; Cabrita, Elsa; Gavaia, Paulo. "Guidelines for zebrafish sperm cryopreservation". Paper presented in Special DiA meeting, 2017.
  9. Diogo, Patrícia; Gil Martins; Eufrásio, Ana; Rita Nogueira; Isa Quinzico; Tomé Silva; Cabrita, Elsa; Gavaia, Paulo. "Guidelines for zebrafish sperm cryopreservation.". Paper presented in 6th International Workshop on the Biology of Fish Gametes, 2017.
  10. Pedro M. Guerreiro; Louro B; Alves A; Couto E; Cruz B; Ana Eufrásio; Deloffre L; Wilson JM; Canario A. "Antarctic fish in a changing world: metabolic, osmoregulatory and endocrine stress response". Paper presented in XV European Congress of Ichthyology, Porto, 2015.
    Accepted
  11. Guerreiro PM; Alves A; Louro B; Wilson JM; Eufrásio, Ana; Canario A. "The renal responses of Notothenia rossi:what makes it tinkle?". Paper presented in 7th Portuguese Conference on PolarSciences, Évora, 2015.
Conference paper
  1. Diogo, Patricia; Eufrásio, Ana; Martins, Gil; Cardeira, João; Cancela, M. Leonor; Cabrita, Elsa; Gavaia, Paulo. "Analysis of sperm quality in a type I diabetes zebrafish model". 2016.
    Published
Conference poster
  1. Nogueira, João M.; Ferreira, Fábio J.; Borges, Sofia; Eufrásio, Ana; Magalhães, Francisco; Sousa, Miguel; Cruz, Mariana; Macedo, Rui; Bessa, Jose. Corresponding author: Bessa, Jose. "Prediction of variant effect in diabetes-associated regulatory elements using random mutagenesis in reporter assays". Paper presented in 15th International Symposium on Variants in the Genome, 2024.
  2. Eufrásio, Ana; Azevedo, Joana; Alexandre Ferreira; Moutinho, Ana; Filipe Henriques; Jesus, Ana; Tavares, Joana; et al. "On the role of a short 3’UTR sequence in gene expression regulation and disease". Paper presented in 30th Porto Cancer Meeting, 2024.
  3. Nogueira, João M.; Ferreira, Fábio J.; Borges, Sofia; Eufrásio, Ana; Cruz, Marta ; Sousa, Miguel; Macedo, Rui; Bessa, Jose. Corresponding author: Bessa, Jose. "A Novel Lentivirus System to Decode the Non-Coding DNA Associated with Type-2 Diabetes Risk". Paper presented in 20th Portugaliæ Genetica: DNA - Ancient and New, 2024.
  4. Machado, Joana; Eufrásio, Ana; Ferreira, Alexandre; Isabel Pereira-Castro; Moreira, Alexandra; Bessa, José. "Unravelling the regulatory function of a short sequence in the 3´UTR in zebrafish and in human cells". Paper presented in IV International Meeting of the Portuguese Society of Genetics, 2023.
  5. Eufrásio, Ana; Perrod, Chiara; J.Ferreira, Fábio; Duque, Marta; Bessa,José. "Genetic variants associated to type 2 diabetes modulate endocrine enhancers in vivo". Paper presented in European Human Genetics Conference 2023 Glasgow, 2023.
  6. Ferreira, Fábio J.; Nogueira, João; Eufrásio, Ana; Santos Cruz, Mariana; Macedo, Rui; Bessa, José. "Cracking the genetic code of Diabetes: a cis-regulatory perspective". Paper presented in EMBL Conference: Chromatin and Epigenetics, Heidelberg, Germany, 2023.
  7. Moutinho, Ana; Eufrásio, Ana; Azevedo, Joana; Joana Teixeira; Machado, Joana; Alexandre Ferreira; Isabel Pereira-Castro; José Bessa; Alexandra Moreira. "Unravelling the function of a short non-coding sequence in gene expression". Paper presented in 4th International Conference on the Long and the Short of Non-Coding RNAs, 2022.
  8. Moutinho, Ana; Eufrásio, Ana; Beatriz C. Garcia; Bessa, Jose; Isabel Pereira-Castro; Alexandra Moreira. "The USE non-coding sequence: on the function and therapeutic applications". Paper presented in IJUP- Young Researchers Meeting 2021, 2021.
  9. Eufrásio, Ana; Perrod, C. AE13-ACCD-75F7; Ferreira, F. J.; Duque, M.; Galhardo, M.; Bessa, J.. "Understanding the impact of cis regulatory disruption in the development of type 2 diabetes". Paper presented in 7th MCBiology Symposium, 2021.
  10. Eufrásio, Ana; Duque, Marta; Bessa, José. "Testing the cis-regulatory potential of type 2 diabetes associated non-coding sequences". Paper presented in EDBC - European Developmental Biology Congress, 2019.
  11. Bordeira-Carriço, Renata; Teixeira, Joana; Duque, Marta; Ribeiro, Diogo; Galhardo, Mafalda; Dominguez-Acemel, R.; Firbas, P.; et al. "Cis-regulatory similarities of human and zebrafish pancreas identify disease related enhancers". Paper presented in EDBC - European Developmental Biology Congress, 2019.
  12. Bordeira-Carriço, Renata; Teixeira, Joana; Duque, Marta; Ribeiro, Diogo; Galhardo, Mafalda; Dominguez-Acemel, R.; Firbas, P.; et al. "Cis-regulatory similarities of human and zebrafish pancreas identify disease related enhancers". Paper presented in From Genes to Organisms: Transcriptional Control in Development and Disease, 2019.
  13. Bordeira-Carriço, Renata; Duque, Marta; Ribeiro, Diogo; Galhardo, Mafalda; Dominguez-Acemel, R.; Firbas, P.; Tena, J.; et al. "Cis-regulatory similarities of human and zebrafish pancreas identify disease related enhancers". Paper presented in Sociedade Portuguesa de Genética Humana Annual Meeting, 2019.
  14. Eufrásio, Ana; Perrod, C. AE13-ACCD-75F7; Ferreira, F. J.; Duque, M.; Galhardo, M.; Bessa, J.. "Testing the cis-regulatory potential of type 2 diabetes associated non-coding sequences.". Paper presented in 8th i3s Annual Meeting, 2019.
  15. Bordeira-Carriço, R; Teixeira, Joana; Duque,M; Ribeiro,D; Galhardo,M; Dominguez-Acemel,R; Panos,F; et al. "Cis-regulatory similarities of the human andzebrafish pancreas identify disease related enhancers". Paper presented in 8th I3SAnnual Meeting. Porto, Portugal, 2019.
  16. Bordeira-Carriço, R; Teixeira, Joana; Duque,M; Ribeiro,D; Galhardo,M; Dominguez-Acemel,R; Panos,F; et al. "The zebrafish pancreas regulome: from prediction to disease". Paper presented in EDBC Congress. Alicante, Spain., 2019.
  17. Eufrásio, Ana; José Bessa. "Testing the cis-regulatory potential of diabetes associated non-coding sequences". Paper presented in SPBD/SEBD/SFBD joint meeting, 2018.
  18. Diogo, Patricia; Eufrásio, Ana; Martins, Gil; Cardeira, João; Cancela, Leonor; Cabrita, Elsa; Gavaia, Paulo. "Analysis of sperm quality in type I diabetes zebrafish model". Paper presented in 20th Annual ESDAR Conference 2016, 2016.
  19. Diogo, Patrícia; Gil Martins; Ana Eufrásio; Rita Nogueira; Isa Quinzico; Tomé Silva ; Elsa Cabrita; Gavaia, Paulo. "Analysis of sperm quality in a type I diabetes zebrafish". Paper presented in European Society for Domestic Animal Reproduction (ESDAR) Annual conference, 2016.
Journal article
  1. Patrícia Diogo; Gil Martins; Márcio Simão; Ana Marreiros; Ana Catarina Eufrásio; Elsa Cabrita; Paulo Jorge Gavaia. "Type I Diabetes in Zebrafish Reduces Sperm Quality and Increases Insulin and Glucose Transporter Transcripts". International Journal of Molecular Sciences (2023): https://doi.org/10.3390/ijms24087035.
    10.3390/ijms24087035
  2. Jorge Matinha-Cardoso; Filipe Coutinho; Steeve Lima; Ana Eufrásio; António Paulo Carvalho; Aires Oliva-Teles; José Bessa; et al. "Novel protein carrier system based on cyanobacterial nano-sized extracellular vesicles for application in fish". Microbial Biotechnology (2022): https://doi.org/10.1111/1751-7915.14057.
    10.1111/1751-7915.14057
  3. Bordeira-Carriço, Renata; Teixeira, Joana; Duque, Marta; Galhardo, Mafalda; Ribeiro, Diogo; Acemel, Rafael D.; Firbas, Panos. N.; et al. "Multidimensional chromatin profiling of zebrafish pancreas to uncover and investigate disease-relevant enhancers". Nature Communications 13 1 (2022): http://dx.doi.org/10.1038/s41467-022-29551-7.
    10.1038/s41467-022-29551-7
  4. Eufrásio, Ana; AE13-ACCD-75F7; Ferreira, Fábio J.; Duque, Marta; Galhardo, Mafalda; Bessa, José. "In Vivo Reporter Assays Uncover Changes in Enhancer Activity Caused by Type 2 Diabetes–Associated Single Nucleotide Polymorphisms". Diabetes 69 12 (2020): 2794-2805. http://dx.doi.org/10.2337/db19-1049.
    10.2337/db19-1049
  5. Diogo, Patrícia; Martins, Gil; Eufrásio, Ana; Silva, Tomé; Cabrita, Elsa; Gavaia, Paulo. "Selection Criteria of Zebrafish Male Donors for Sperm Cryopreservation". Zebrafish (2018): http://dx.doi.org/10.1089/zeb.2018.1660.
    10.1089/zeb.2018.1660
Preprint
  1. Eufrásio, Ana; Azevedo, Joana; Machado, Joana; Ferreira, Alexandre; Moutinho, Ana; Henriques, Filipe; Jesus, Ana; et al. "A short 3’UTR motif regulates gene expression in bilaterians". 2023. http://dx.doi.org/10.1101/2023.11.15.567165.
    10.1101/2023.11.15.567165
Thesis / Dissertation
  1. "Understanding the impact of cis-regulatory disruption in the development of type 2 diabetes". PhD, Universidade do Porto Instituto de Investigação e Inovação em Saúde, 2024.
  2. Eufrásio, Ana. "Testing the cis-regulatory potential of type 2 diabetes associated non-coding sequences". Master, Universidade de Aveiro, 2018.

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2024 - Current Portuguese Glial Network (2024 - 2025/05)
Symposium (Member of the Organising Committee)
2023 - 2023 Mostra UP Porto 2023 - Participation in the event representing i3S - Institute for Research and Innovation in Health of the University of Porto (2023 - 2023)
Exhibition (Member of the Scientific Committee)
 Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021/11/30 - 2021/11/30 Support in organizing the 4th PhDay. (2021/11/30 - 2021/11/30)
Congress (Co-organisor)
 Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021/01/29 - 2021/01/29 Support in organizing the 7th Molecular and Cell Biology Symposium - Science Must Go On. (2021/01/29 - 2021/01/29)
Congress (Co-organisor)
 Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2018 - 2018 SPBD, SEBD, SFBD international joint meeting - Support in organizing the event. Placement of posters, secretariat. (2018)
Conference (Member of the Organising Committee)

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2023 - 2023 As mosquinhas vão à escola - Participation in the workshop held within the framework of the Ciência et al school program of i3S
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021 - 2021 Joint event between the BSHP and the Faculty for the History and Philosophy of Medicine and Pharmacy of the Society of Apothecaries.
Symposium
 Unlocking the Medieval Medicine Cabinet
2020 - 2020 Webinar Development presents...
Seminar
 Development presents...
2020 - 2020 Webinar ORGANOIDS WITHOUT BORDERS
Seminar
 ORGANOIDS WITHOUT BORDERS
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2020 - 2020 Covid19 Diagnosis Taskforce - Computational operations
Other
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019 - 2019 Publishing Workshop for Authors and Reviewers - Elsevier Publishing Workshop for Authors and Reviewers - Elsevier
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2019 - 2019 Organoids Workshop - Stem Cell Workshop em organóides - Stem Cell
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2024/12/11 Decoding the endocrine pancreas regulome and its implications in type 2 diabetes development
(Thesis) Main arguer
 Diogo Sousa (Master)
Universidade do Porto Faculdade de Ciências, Portugal

Association member

Society Organization name Role
2019 - Current Sociedade Portuguesa de Biologia do Desenvolvimento (SPBD)

Conference scientific committee

Conference name Conference host
2024/10 - 2024/10 7th PhDay - From Theory to Application: Research in Action i3s, Porto

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2023/05 - 2023/05 Hands-on course in transgenesis and mutagenesis in zebrafish - 1st edition Hands-on course in transgenesis and mutagenesis in zebrafish – 1st edition Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Mentoring / Tutoring

Topic Student name
2023 - 2023 Genetic variants associated to type 2 diabetes modulate endocrine coding enhancers in vivo Jovel Jose
2021 - 2022 Zebrafish microinjection and confocal microscopy Ana Moutinho
2021 - 2021 Zebrafish microinjection and confocal microscopy Inês Pinto

Award

2019 Best poster award at the 8th i3S Annual Meeting