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Leonardo Mendes e Silva completed his Master's in Biological Engineering in 2015 at Universidade do Algarve, Faculty of Sciences and Technology, and obtained his Ph.D. in Mechanisms of Disease and Regenerative Medicine in 2023 from UAlg/Universidade NOVA de Lisboa, NOVA Medical School. Currently, he serves as a Researcher at Pattern Institute and an Analytical Scientist at Hovione. He has published 9 articles in journals, and participated in 5 events. Leonardo has co-supervised 3 BSc completion projects and received 1 award. He has been involved in 1 project as a PhD Student Fellow and 1 project as a Research Fellow. His expertise lies in Natural Sciences, particularly Biological Sciences. Throughout his career, he has collaborated with 61 individuals on scientific papers. In his Ciência Vitae curriculum, key terms include stem cells, cell therapy, cardiogenesis, pluripotency, zebrafish, regenerative medicine, iPSC, genomics, epigenetics, computanional biology, R, Python.
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Identification

Personal identification

Full name
Leonardo Mendes e Silva

Citation names

  • Mendes-Silva, Leonardo
  • Mendes e Silva, Leonardo
  • Silva, Leonardo

Author identifiers

Ciência ID
7918-CF94-9CF4
ORCID iD
0000-0002-5989-637X

Email addresses

  • lmesilva@pattern.institute (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences
  • Exact Sciences - Computer and Information Sciences - Bioinformatics
Education
Degree Classification
2023/09/04 - 2023/09/15
Concluded
 Summer School in Computational Biology (Curso de Especialização Tecnológica)
Universidade de Coimbra, Portugal
2022/09/23 - 2023/03/06
Concluded
 Data Science Course 2023: Complete Data Science Bootcamp (Curso de Especialização Tecnológica)
Udemy Inc, United States
2018/03/15 - 2023/01/16
Concluded
 Mechanisms of Disease and Regenerative Medicine (Doutoramento)
Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal

Universidade NOVA de Lisboa NOVA Medical School, Portugal
"From pluripotency to early events leading to cardiogenesis" (THESIS/DISSERTATION)
2021/05/17 - 2021/05/21
Concluded
 Smart Microscopy Workshop (Curso de Especialização Tecnológica)
Göteborgs Universitet, Sweden
2018/06/04 - 2018/06/05
Concluded
 R for Absolute Beginners 6th edition (Curso de Especialização Tecnológica)
Centro de Ciências do Mar, Portugal
Affiliation

Science

Category
Host institution 		Employer
2023/09/19 - Current Researcher (Research) Pattern Institute, Portugal
Pattern Institute, Portugal
2016/01/04 - 2016/08/31 Researcher (Research) Centro de Electronica Optoelectrônica e Telecomunicacoes, Portugal

Others

Category
Host institution 		Employer
2023/11/06 - Current Analytical Scientist Hovione, Portugal
Hovione, Portugal
2018/03/15 - 2023/01/16 PhD student Universidade do Algarve - Campus de Gambelas, Portugal
Universidade do Algarve Centro de Investigação em Biomedicina, Portugal
2017/10/01 - 2018/03/14 Junior Researcher 2Siglas- Formação e Investigação, Portugal
2016/09/06 - 2017/04/30 Junior Researcher 2Siglas- Formação e Investigação, Portugal
2014/02/01 - 2014/07/31 Msc dissertation: Screening of process conditions for the production of biogas from diluted organic waste streams using microreactors. Rijksuniversiteit Groningen, Netherlands
2013/09/01 - 2014/01/15 Trainee-ship under the supervision of Prof. Dr. Eduardo Melo Universidade do Algarve Centro de Investigação em Biomedicina, Portugal
Projects

Grant

Designation Funders
2018/03/15 - Current From pluripotency to early events leading to cardiogenesis
PD/BD/137070/2018
PhD Student Fellow
Universidade do Algarve Centro de Investigação em Biomedicina, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2020/04/30 - 2020/10/30 Development of a new assay do detect SARS-CoV-2 directly from clinical samples
126_596760136
Research Fellow
Fundação para a Ciência e a Tecnologia
Ongoing

Other

Designation Funders
2018/09/01 - 2020/06/01 VITAL: Variant sequences Identification genome wide and Their Assignment as true causative mutations for LVNC
ALG-01-0145-FEDER-28044
Researcher
Universidade do Algarve, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
Outputs

Publications

Book
  1. Cavaco, A.M.; Antunes, M.D.; Panagopoulos, T.; Rosendo, M.; Pires, R.; Brázio, A.; Silva, L.; Afonso, A.M.; Guerra, R.. Upgrading current practices in Vis/NIR spectroscopy for in situ orchard monitoring. 2018.
    10.17660/ActaHortic.2018.1194.106
  2. Cavaco, A.M.; Antunes, M.D.; Guerra, R.; Rosendo, M.; Pires, R.; Brázio, A.; Silva, L.; et al. Fruit quality survey techniques to optimize crop management in citrus orchards. 2018.
    10.17660/ActaHortic.2018.1194.21
Conference paper
  1. Mendes-Silva, Leonardo; Rui Guerra. "A possible upgrade of the Algarve Citrus protected geographical indication norm". Paper presented in IX Simpósio Ibérico de Maturação e Pós-Colheita, Lisbon, 2016.
    Published
Conference poster
  1. Fernandes, Mónica T; Mendes-Silva, Leonardo; Sofia M. Calado; Bragança, José. "Exploring CITED2 role in the stemness properties of glioblastoma cancer stem cells.". Paper presented in 12th International Meeting of the Portuguese Society for Stem Cells and Cell Therapies, 2022.
  2. Fernandes, Mónica T; Mendes-Silva, Leonardo; Sofia M. Calado; Bragança, José. "Evaluation of the expression of potential CITED2-interacting partners in glioblastoma cancer stem cells". Paper presented in Meeting ASPIC-ASEICA (Portuguese and Spanish Associations for Cancer Research), 2021.
  3. Ana M. Cavaco; Pires, Rosa; Guerra, Rui Manuel Farinha das Neves; Antunes, M. Dulce; Cruz, Sandra P.; Panagopoulos, Thomas; Brázio, António; et al. Corresponding author: Ana M. Cavaco. "The perks and ills of Vis-NIR spectroscopy in the assessment of Algarve Citrus ripening on-tree". Paper presented in 1st sensorFINT International Workshop of the COST Action 19145 ¿EuropeanNetwork for assuring food integrity using non-destructive spectral sensors¿ dedicated to ¿Smart Spectral Sensors for Agri-Food Quality and Process Control¿, 2021.
  4. Mendes-Silva, Leonardo. "Cited2 induced secretome and its cardiogenic potential". Paper presented in 3ªs Jornadas do ABC, 2019.
  5. Mendes-Silva, Leonardo. "Zebrafish as model to study Cited2-caused developmental defects". Paper presented in Secretome Without Borders, 2018.
  6. Mendes-Silva, Leonardo. "Cited2 in cardiac development: an inside and outside job". Paper presented in Secretome Without Borders, 2018.
  7. Mendes-Silva, Leonardo. "Case study of shelf life of sweet-potato king's cake". Paper presented in 7th Congress of European Microbiologists, 2017.
  8. Mendes-Silva, Leonardo; Rui Guerra. "Effect of orange peel in whole oranges' spectra". Paper presented in IX Simpósio Ibérico de Maturação e Pós-Colheita, 2016.
  9. Mendes-Silva, Leonardo. "Nondestructive Determination of SSC and pH of Pear Using Vis-NIR Spectroscopy". Paper presented in VIII International Postharvest Symposium., 2016.
    10.13031/aim.20141829994
Journal article
  1. Martins, J.A.; Guerra, R.; Pires, R.; Antunes, M.D.; Panagopoulos, T.; Brázio, A.; Afonso, A.M.; et al. "SpectraNet–53: A deep residual learning architecture for predicting soluble solids content with VIS–NIR spectroscopy". Computers and Electronics in Agriculture 197 (2022): http://www.scopus.com/inward/record.url?eid=2-s2.0-85128737131&partnerID=MN8TOARS.
    10.1016/j.compag.2022.106945
  2. Cavaco, Ana M.; Cruz, Sandra P.; Antunes, M. Dulce; Guerra, Rui; Pires, Rosa; Afonso, Andreia M.; Brázio, António; et al. "Spatiotemporal modelling of the quality and ripening of two cultivars of "Algarve Citrus" orchards at different edaphoclimatic conditions". Postharvest Biology and Technology 172 (2021): 111386. http://dx.doi.org/10.1016/j.postharvbio.2020.111386.
    Published • 10.1016/j.postharvbio.2020.111386
  3. Calado, S.M.; Bento, D.; Justino, D.; Mendes-Silva, L.; Marques, N.; Bragança, J.. "Generation of a human induced pluripotent stem cell line (UALGi001-A) from a patient with Left-Ventricular Noncompaction Cardiomyopathy". Stem Cell Research 53 (2021): http://www.scopus.com/inward/record.url?eid=2-s2.0-85103373595&partnerID=MN8TOARS.
    10.1016/j.scr.2021.102302
  4. Fernandes, Mónica T; Calado, Sofia M; Mendes-Silva, Leonardo; Bragança, José. "CITED2 and the modulation of the hypoxic response in cancer". World Journal of Clinical Oncology 11 5 (2020): 260-274. http://dx.doi.org/10.5306/wjco.v11.i5.260.
    10.5306/wjco.v11.i5.260
  5. João M. A. Santos; Leonardo Mendes-Silva; Vanessa Afonso; Gil Martins; Rui S. R. Machado; João A. Lopes; Leonor Cancela; et al. "Exogenous WNT5A and WNT11 proteins rescue CITED2 dysfunction in mouse embryonic stem cells and zebrafish morphants". Cell Death & Disease 10 8 (2019): https://doi.org/10.1038/s41419-019-1816-6.
    10.1038/s41419-019-1816-6
  6. Mendes-Silva, Leonardo. "CITED Proteins in the Heart of Pluripotent Cells and in Heart’s Full Potential". Regenerative Medicine Frontiers (2019): http://dx.doi.org/10.20900/rmf20190005.
    10.20900/rmf20190005
  7. Bragança, José; Lopes, João André; Mendes-Silva, Leonardo; Santos, João Miguel Almeida. "Induced pluripotent stem cells, a giant leap for mankind therapeutic applications". World Journal of Stem Cells 11 7 (2019): 421-430. http://dx.doi.org/10.4252/wjsc.v11.i7.421.
    10.4252/wjsc.v11.i7.421
  8. Cavaco, A.M.; Pires, R.; Antunes, M.D.; Panagopoulos, T.; Brázio, A.; Afonso, A.M.; Silva, L.; et al. "Validation of short wave near infrared calibration models for the quality and ripening of ‘Newhall’ orange on tree across years and orchards". Postharvest Biology and Technology 141 (2018): 86-97. http://www.scopus.com/inward/record.url?eid=2-s2.0-85044481284&partnerID=MN8TOARS.
    10.1016/j.postharvbio.2018.03.013
  9. Tsunoda, S.; Avezov, E.; Zyryanova, A.; Konno, T.; Mendes-Silva, L.; Melo, E.P.; Harding, H.P.; Ron, D.. "Intact protein folding in the glutathione-depleted endoplasmic reticulum implicates alternative protein thiol reductants". eLife 2014 3 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84905170031&partnerID=MN8TOARS.
    10.7554/eLife.03421.001
Magazine article
  1. Mendes-Silva, Leonardo. "Os poderes secretos das células estaminais para limitar as malformações do coração", UAlgoritmo, 2020, https://ualgoritmo.wixsite.com/website/c%C3%B3pia-u2-2-7.
Newspaper article
  1. Mendes-Silva, Leonardo. "Citrinos do Algarve IGP: porquê e como melhorar a respetiva norma?", PH- Revista da Associação Portuguesa de Horticultura, 2016
Online resource
  1. Mendes-Silva, Leonardo. Prevention of congenital heart anomalies: the secret kept by embryonic stem cells. 2021. https://atlasofscience.org/prevention-of-congenital-heart-anomalies-the-secret-kept-by-embryonic-stem-cells/.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2019/03/28 Zebrafish as a model to study cited2-caused developmental defects 3ªs Jornadas do ABC
Algarve Biomedical Center (Albufeira, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2021/05/01 - 2021/07/01 qPCR validation of genes differentially expressed in embryonic stem cells in response to decreased Cited2 expression
Co-supervisor
 Ciências Biomédicas (Degree)
Universidade do Algarve Faculdade de Ciências e Tecnologia, Portugal
2019/09/15 - 2020/04/25 Validação por qPCR de genes diferencialmente expressos nas células estaminais embrionárias em resposta à diminuição da expressão de Cited2
Co-supervisor
 Bioquímica (Other)
Universidade do Algarve Faculdade de Ciências e Tecnologia, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2022/11/07 - 2022/11/11 During this innovation event, participants were divided into interdisciplinary workgroups tasked with addressing real challenges presented by hospital managers from the central region of Portugal. Over the course of four days, our group collaborated on developing a solution to the challenge assigned to us. On the final day of the event, I had the opportunity to co-present half of the pitch alongside a colleague, articulating our proposed solution. Additionally, we effectively defended our idea against inquiries from the jury panel, showcasing our ab
Symposium
 Cantanhede Innovation Days 2022
Biocant Associação de Transferência de Tecnologia, Portugal
2021/10/13 - 2021/10/13 At this event, I participated by managing the filming process and single-handedly handling all video editing tasks for the promotional video related to "Dia das Células Estaminais 2021". (https://youtu.be/RNZIqOkCWWE?si=VlNk_iiWrh8_f4Lm)
Other
 Dias das Célula Estaminais
Sociedade Portuguesa de Células Estaminais e Terapia Celular, Portugal

Universidade do Algarve, Portugal
2020/04/01 - 2020/06/30 Volunteer for COVID-19 Diagnostic Tests at CBMR in collaboration with ABC and ARS-Algarve
Other
Universidade do Algarve, Portugal

Universidade do Algarve Centro de Investigação em Biomedicina, Portugal
2020/02/20 - 2020/02/20 Collaborator in the UAlg Open Day for high school students
Other
Universidade do Algarve Centro de Investigação em Biomedicina, Portugal
2019/10/10 - 2019/10/12 Participation at SPCE-TC
Conference
 11th International Meeting of the SPCE-TC
Universidade de Lisboa Faculdade de Farmácia, Portugal

Association member

Society Organization name Role
2018/11 - Current Sociedade Portuguesa de Células Estaminais e Terapia Celular

Mentoring / Tutoring

Topic Student name
2021/06/01 - 2021/07/12 Estágio Optativo em Laboratório Cátia Correia
2019/09/15 - 2019/12/20 Validação por qPCR de genes diferencialmente expressos nas células estaminais embrionárias em resposta à diminuição da expressão de Cited2 Daniela Botinas
2019/03/01 - 2019/06/29 Validação por qPCR de genes diferencialmente expressos nas células estaminais embrionárias em resposta à diminuição da expressão de Cited2 Anita Ferreira
Distinctions

Award

2018 PhD fellowship
Fundação para a Ciência e a Tecnologia, Portugal