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Dalinda Eusébio completed her BSc in Biotechnology (2016) and her MSc in Biomedical Sciences (2018) at the University of Beira Interior, Covilhã, Portugal. Currently, she is working on her Doctoral Program in Biomedicine, at the Health Sciences Research Centre of the University of Beira Interior (CICS-UBI) in collaboration with The University of Texas at Austin, with a Ph.D. fellowship from the Foundation for Science and Technology (FCT). Her research work is focused on the pharmaceutical nanobiotechnology area, particularly on the production, purification, and targeted delivery of the innovative minicircle DNA (mcDNA) vector. The mcDNA delivery has been explored for gene therapy and DNA vaccine applications, including treatments for cervical cancer and coronavirus disease (COVID-19).
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Identificação

Identificação pessoal

Nome completo
Dalinda Isabel da Silva Eusébio
Data de nascimento
1995/02/10
Género
Feminino

Nomes de citação

  • Dalinda Eusébio

Identificadores de autor

Ciência ID
1719-3CAA-4905
ORCID iD
0000-0003-3292-2184

Endereços de correio eletrónico

  • dalinda.eusebio@ubi.pt (Profissional)

Moradas

  • RISE-Health, Department of Medical Sciences, Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Covilhã, Portugal (Profissional)

Domínios de atuação

  • Ciências Médicas e da Saúde - Biotecnologia Médica - Diagnóstico e Terapias de Base Genética

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador independente (B2) Utilizador independente (B2) Utilizador independente (B2) Utilizador independente (B2)
Espanhol; Castelhano Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1) Utilizador independente (B1)
Formação
Grau Classificação
2019 - 2025
Em curso
 Biomedicine (Doutoramento)
Universidade da Beira Interior, Portugal
"Mannosylated minicircle DNA nanovaccine against COVID-19" (TESE/DISSERTAÇÃO)
2018/11/07
Concluído
 Biomedical Sciences (Mestrado)
Universidade da Beira Interior, Portugal
"Multigenic minicircle DNA with potential therapeutic action in cervical cancer" (TESE/DISSERTAÇÃO)
 18
2016/07/01
Concluído
 Biotechnology (Licenciatura)
Universidade da Beira Interior, Portugal
"DNA vectors for the expression of microRNA 375 in the treatment of cervical cancer" (TESE/DISSERTAÇÃO)
 15
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento 		Empregador
2017 - Atual Investigador (Investigação) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Projetos

Bolsa

Designação Financiadores
2021/01 - Atual Mannosylated minicircle DNA nanovaccine against COVID-19
2020.10201.BD
Bolseiro de Doutoramento
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Em curso
2022/10 - 2022/12 Short-Term Research Internship Program at The University of Texas at Austin
UT Austin Portugal Program
Bolseiro de Investigação
The University of Texas at Austin Dell Pediatric Research Institute, Estados Unidos
 Fundação para a Ciência e a Tecnologia
Concluído
2020/02 - 2020/12 Obtainment and targeted delivery of a minicircle DNA vaccine against Human Papillomavirus-associated cervical cancer
BID/FCS/2019
Bolseiro de Doutoramento
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
 Banco Santander Portugal
Concluído
Produções

Publicações

Artigo em revista
  1. Inês Afonso; Ana R. Neves; Dalinda Eusébio (1719-3CAA-4905); Tânia Albuquerque; Eric Vivès; Prisca Boisguérin; Adriana O. Santos; Ângela Sousa; Diana Costa. "Design of Experiments to Tailor the Potential of BSA-Coated Peptide Nanocomplexes for Temozolomide/p53 Gene Co-Delivery". Pharmaceutics 16 11 (2024): 1389. https://doi.org/10.3390/pharmaceutics16111389.
    10.3390/pharmaceutics16111389
  2. Dalinda Eusébio (1719-3CAA-4905); Milan Paul; Swati Biswas; Zhengrong Cui; Diana Costa; Ângela Sousa. "Mannosylated polyethylenimine-cholesterol-based nanoparticles for targeted delivery of minicircle DNA vaccine against COVID-19 to antigen-presenting cells". International Journal of Pharmaceutics 654 (2024): 123959. http://dx.doi.org/10.1016/j.ijpharm.2024.123959.
    10.1016/j.ijpharm.2024.123959
  3. Cathy Ventura; Dalinda Eusébio (1719-3CAA-4905); Ana M. Gonçalves; Jorge Barroca-Ferreira; Diana Costa; Zhengrong Cui; Luís A. Passarinha; Ângela Sousa. "Maximization of the Minicircle DNA Vaccine Production Expressing SARS-CoV-2 RBD". Biomedicines 10 5 (2022): 990. http://dx.doi.org/10.3390/biomedicines10050990.
    10.3390/biomedicines10050990
  4. Dalinda Eusébio (1719-3CAA-4905); Ana R. Neves; Diana Costa; Swati Biswas; Gilberto Alves; Zhengrong Cui; Ângela Sousa. "Methods to improve the immunogenicity of plasmid DNA vaccines". Drug Discovery Today 26 11 (2021): 2575-2592. http://dx.doi.org/10.1016/j.drudis.2021.06.008.
    10.1016/j.drudis.2021.06.008
  5. Carlos Rodolfo; Dalinda Eusébio (1719-3CAA-4905); Cathy Ventura; Renato Nunes; Helena F. Florindo; Diana Costa; Ângela Sousa. "Design of Experiments to Achieve an Efficient Chitosan-Based DNA Vaccine Delivery System". Pharmaceutics 13 9 (2021): 1369. http://dx.doi.org/10.3390/pharmaceutics13091369.
    10.3390/pharmaceutics13091369
  6. Ana Sofia Serra; Dalinda Eusébio (1719-3CAA-4905); Ana Raquel Neves; Tânia Albuquerque; Himanshu Bhatt; Swati Biswas; Diana Costa; Ângela Sousa. "Synthesis and characterization of mannosylated formulations to deliver a minicircle DNA vaccine". Pharmaceutics 13 5 (2021): 673. https://doi.org/10.3390/pharmaceutics13050673.
    https://doi.org/10.3390/pharmaceutics13050673
  7. Dalinda Eusébio (1719-3CAA-4905); Ana M. Almeida; Joel M. Alves; Cláudio J. Maia; João A. Queiroz; Fani Sousa; Ângela Sousa. "The Performance of Minicircle DNA Versus Parental Plasmid in p53 Gene Delivery Into HPV-18-Infected Cervical Cancer Cells". Nucleic Acid Therapeutics 31 1 (2021): 82-91. http://dx.doi.org/10.1089/nat.2020.0904.
    10.1089/nat.2020.0904
  8. Ana M. Almeida; Dalinda Eusébio (1719-3CAA-4905); João A. Queiroz; Fani Sousa; Ângela Sousa. "The use of size-exclusion chromatography in the isolation of supercoiled minicircle DNA from Escherichia coli lysate". Journal of Chromatography A 1609 (2020): 460444-460444. https://doi.org/10.1016/j.chroma.2019.460444.
    10.1016/j.chroma.2019.460444
  9. Joana Coelho; Dalinda Eusébio (1719-3CAA-4905); Diana Gomes; Filipe Frias; Luís A. Passarinha; Ângela Sousa. "Biosynthesis and isolation of gellan polysaccharide to formulate microspheres for protein capture". Carbohydrate Polymers 220 (2019): 236-246. https://doi.org/10.1016/j.carbpol.2019.05.011.
    10.1016/j.carbpol.2019.05.011
Capítulo de livro
  1. Dalinda Eusébio (1719-3CAA-4905); Andressa Giusti; Matilde Costa; Ângela Sousa. "Production, Purification, and Delivery of Minicircle DNA Encoding p53 Using Polyethyleneimine for Cancer Gene Therapy". In Therapeutic Genome Editing - Methods in Molecular Biology. Springer, 2025.
    Submetido
  2. Dalinda Eusébio (1719-3CAA-4905); Tânia Albuquerque; Ana R. Neves; Rúben Faria ; Cathy Ventura; Miguel Ferreira; Ângela Sousa; Diana Costa. "Drug delivery for bone tissue engineering". In Advanced and Modern Approaches for Drug Delivery, 783-815. Elsevier, 2023.
  3. Ana R. Neves; Tânia Albuquerque; Dalinda Eusébio (1719-3CAA-4905); Ângela Sousa; Diana Costa. "Challenges and future perspectives of natural biopolymers in drug delivery". In Natural Biopolymers for Drug Delivery. Elsevier, 2023.
  4. Ana M. Almeida; Dalinda Eusébio (1719-3CAA-4905); João A. Queiroz; Fani Sousa; Ângela Sousa. "Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment". In DNA vaccines: Methods and Protocols, 207-222. New York, Estados Unidos: Humana Press, 2020.
Poster em conferência
  1. Dalinda Eusébio (1719-3CAA-4905); Milan Paul; Swati Biswas; Zhengrong Cui; Diana Costa; Ângela Sousa. "Mannosylated chitosan-octa-arginine-based nanoparticles for targeted delivery of minicircle DNA vaccine against COVID-19 to antigen-presenting cells". Trabalho apresentado em Congress of Microbiology and Biotechnology, 2023.
  2. Dalinda Eusébio (1719-3CAA-4905); Milan Paul; Swati Biswas; Zhengrong Cui; Diana Costa; Ângela Sousa. "Targeted delivery of minicircle DNA vaccine against COVID-19 to antigen-presenting cells using mannosylated polyethylenimine-cholesterol-based nanoparticles". Trabalho apresentado em Congress of Microbiology and Biotechnology, 2023.
  3. Dalinda Eusébio (1719-3CAA-4905); Cathy Ventura; Ana M. Gonçalves; Jorge Barroca-Ferreira; Diana Costa; Zhengrong Cui; Luís A. Passarinha; Ângela Sousa. "Optimization of the minicircle DNA vaccine expressing SARS-CoV-2 RBD production using orbital shaker experiments and a mini-bioreactor platform". Trabalho apresentado em IV International Congress on Health Sciences Research Towards Innovation and Entrepreneurship - Challenges from Research to Clinics, 2023.
  4. Dalinda Eusébio (1719-3CAA-4905); Milan Paul; Swati Biswas; Zhengrong Cui; Diana Costa; Ângela Sousa. "Multifunctional nanoparticles for targeted delivery of minicircle DNA vaccine against COVID-19 to antigen-presenting cells". Trabalho apresentado em XVII International CICS-UBI Symposium, 2022.
  5. Ana Sofia Serra; Dalinda Eusébio (1719-3CAA-4905); Ana Raquel Neves; Diana Costa; Ângela Sousa. "Formulation of R8-based nanosystems to delivery a minicircle DNA vaccine to dendritic cells". Trabalho apresentado em XV Annual CICS-UBI Symposium, 2020.
  6. Diana C. Pereira; Adriana R. Pinto; Dalinda Eusébio (1719-3CAA-4905); Ana M. Almeida; João A. Queiroz; Fani Sousa; Ângela Sousa. "Production and Purification of minicircle DNA vectors for use in cervical cancer cells". Trabalho apresentado em III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Aging and Cancer, 2019.
  7. Adriana R. Pinto; Diana C. Pereira; Dalinda Eusébio (1719-3CAA-4905); Ana M. Almeida; João A. Queiroz; Luís Passarinha; Ângela Sousa. "Evaluation of tumor suppressor and HPV oncoprotein expression in cervical cancer cells by using minicircle DNA vectors". Trabalho apresentado em III International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Aging and Cancer, 2019.
  8. Dalinda Eusébio (1719-3CAA-4905); Ana M. Almeida; Joel M. Alves; Cláudio J. Maia; João A. Queiroz; Fani Sousa; Ângela Sousa. "Recombinant p53 minicircle DNA: from vector construction to biopharmaceutical purification". Trabalho apresentado em 12th Symposium of the European Society of Biochemical Engineering Sciences (ESBES), 2018.
  9. Dalinda Eusébio (1719-3CAA-4905); Ana M. Almeida; Joel M. Alves; Cláudio J. Maia; João A. Queiroz; Fani Sousa; Ângela Sousa. "Recombinant p53 minicircle DNA: biopharmaceutical purification and re-establishing of the p53 levels in cancer cells.". Trabalho apresentado em XIII Annual CICS-UBI Symposium, 2018.
Website
  1. Dalinda Eusébio (1719-3CAA-4905); Ângela Sousa. The performance of minicircle DNA versus parental plasmid in p53 gene delivery into HPV-18-infected cervical cancer cells. 2021. http://www.aspic.pt/pt-pt/compara%C3%A7%C3%A3o-entre-os-vetores-de-dna-minicircular-e-plasm%C3%ADdeo-parental-na-entrega-do-gene-p53-c%C3%A9lulas#.YCpLCGj7TIU.

Propriedade Intelectual

Patente
  1. Dalinda Eusébio (1719-3CAA-4905); Swati Biswas; Diana Costa; Ângela Sousa. 2025. "NANOSSISTEMAS HÍBRIDOS PARA ENTREGA DE VACINAS DE ÁCIDOS NUCLEICOS". Portugal.
    Pendente
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2024/10/26 Targeted delivery of a minicircle DNA vaccine against COVID-19 to antigen-presenting cells using mannosylated polyethylenimine-cholesterol-based nanoparticles 5th Edition of International Vaccines Congress (IVC 2024)
2021/09/30 Optimization of minicircle DNA recombination conditions exploring design of experiments using erlenmerys XVI Annual CICS-UBI Symposium
University of Beira Interior (Covilhã, Portugal)
2021/05/22 Development of a minicircle DNA vaccine against COVID-19 XI National Biomedical Sciences Conference
University of Beira Interior (Covilhã, Portugal)
2020/10/01 The performance of minicircle DNA versus parental plasmid in p53 gene delivery into HPV-18-infected cervical cancer cells XV Annual CICS-UBI Symposium
University of Beira Interior (Covilhã, Portugal)
2019/09 Gellan exopolysaccharide biosynthesis and recovery to formulate microspheres optimized by Design of experiment 13th International Meeting of the Portuguese Carbohydrate Group (GLUPOR13)
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto (Porto, Portugal)
2019/07 Therapeutic effect evaluation of minicircle DNA (mcDNA vectors) XIV Annual CICS-UBI Symposium
Universidade da Beira Interior (Covilhã, Portugal)

Orientação

Título / Tema
Papel desempenhado 		Curso (Tipo)
Instituição / Organização
2023 - Atual Development of DNA nanovaccines based on functionalized RALA and Chitosan nanoparticles bearing HPV-16 oncogenes
Coorientador de Andressa Moreira Giusti
Universidade da Beira Interior, Portugal

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação) 		Instituição / Organização
2024 - 2024 Organization of the activity "How to Produce a Vaccine in the Laboratory?" within the scope of the STEAM Junior Academy. (2024/01)
Oficina (workshop) (Coorganizador)
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2023 - 2023 Organization of the activity 'How to Obtain a DNA Vaccine?' within the scope of Science and Technology Week. (2023/11 - 2023/11)
Oficina (workshop) (Coorganizador)
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2021 - 2023 IV International Congress on Health Sciences Research Towards Innovation and Entrepreneurship - Challenges from Research to Clinics. (2023/02/13 - 2023/02/15)
Congresso (Membro da Comissão Organizadora)
 Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
2022/07/18 - 2022/07/22 Organization of the activity "Different Therapeutic Approaches Against Cervical Cancer" within the scope of the Ciência Viva no Laboratório program. (2022)
Oficina (workshop) (Coorganizador)
 Universidade da Beira Interior Centro de Investigação em Sistemas Electromecatrónicos, Portugal
2021/07/19 - 2021/07/23 Organization of the activity "Preparation of a Mannosylated Nanovaccine Against COVID-19" within the scope of the Ciência Viva no Laboratório program. (2021)
Oficina (workshop) (Coorganizador)
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2021 - 2021 Organization of the activity 'Rally of Biopharmaceuticals' within the scope of Science and Technology Week. (2021/11/23 - 2021/11/23)
Oficina (workshop) (Coorganizador)
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2019 - 2019 Organization of the activity 'Game Lab - CICS UBI - Discover the Criminal' within the scope of Science and Technology Week. (2019/11 - 2019/11)
Oficina (workshop) (Coorganizador)
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2017 - 2017 VI Symposium on Employment and Opportunities in Biomedical Sciences. (2017/05/24 - 2017/05/24)
Simpósio (Membro da Comissão Organizadora)
 Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal

Participação em evento

Descrição da atividade
Tipo de evento 		Nome do evento
Instituição / Organização
2022/05/13 - 2022/05/13 Academic and professional perspective of students and teachers.
Mesa-redonda
 VI Cycle of Conferences on Biotechnology
Universidade da Beira Interior, Portugal
2018/07/05 - 2018/07/06 XIII Annual CICS-UBI Symposium
Simpósio
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2017/05/17 - 2017/05/20 II International Congress in Health Sciences Research: Towards Innovation and Entrepreneurship - Trends in Biotechnology for Biomedical Application
Congresso
Universidade da Beira Interior, Portugal
2017/04/06 - 2017/04/06 III Conference Series in Biotechnology – Medicinal and Pharmaceutical
Conferência
Universidade da Beira Interior, Portugal
2017/03/08 - 2017/03/11 VIII National Biomedical Sciences Conference
Conferência
Universidade da Beira Interior, Portugal
2016/04/29 - 2016/04/29 II Conference Series in Biotechnology – Environmental and Ecotoxicology
Conferência
Universidade da Beira Interior, Portugal
2016/03 - 2016/03 II Biomedical Conference Series in the Afternoon
Conferência
Universidade da Beira Interior, Portugal
2015/02/27 - 2015/02/27 I Conference Series in Food Safety and Biotechnology
Conferência
Universidade da Beira Interior, Portugal

Curso / Disciplina lecionado

Disciplina Curso (Tipo) Instituição / Organização
2023/06/26 - 2023/07/13 Completed Training on Laboratory Animal Procedures Theoretical component (56 hours) Universidade da Beira Interior, Portugal
2023/07/06 - 2023/07/08 Completed Training on Laboratory Animal Procedures Practical component (60 hours) Universidade da Beira Interior, Portugal

Entrevista (jornal / revista)

Descrição da atividade Jornal / Forum
2021/11/21 Portuguese Vaccines: What Stage Are They at and the Funding Issue. Teams Attempting to Develop COVID-19 Vaccines in Earlier Development Phases. Jornal Público
2020/12/25 A young portuguese woman from Pombal is developing an innovative vaccine to prevent and treat COVID-19, which promises to be more effective and much more affordable. Jornal Região de Leiria
2020/08/22 Interview with Dalinda Eusébio, PhD Student/Scholar in Biomedicine at the University of Beira Interior: "We hope that our vaccine will be effective for both infected and non-infected individuals". Jornal O Interior
2020/08/17 PhD student in Biomedicine at the University of Beira Interior, awarded a research grant for the development of a preventive and therapeutic nanovaccine against COVID-19. Revista Digital Viva Serra
2020/08/12 A Portuguese woman is developing an innovative vaccine for COVID-19. A young PhD student from the University of Beira Interior has received a grant to create a vaccine that, in addition to being preventive, also aims to be therapeutic. Diário de Notícias
2020/08 A young woman from Pombal receives a grant to create a vaccine for COVID-19. Jornal de Pombal

Entrevista / Programa (rádio / tv)

Programa Tema
2021/02/09 - 2021/02/09 É Ou Não É? - O Grande Debate - RTP1 In Search of Immunity - Debate on the current status of vaccination in Portugal.
2020/12/05 - 2020/12/05 Flagrante Direto - Rádio Cova da Beira Researcher at UBI is working on the creation of an innovative COVID-19 vaccine.
2020/11/21 - 2020/11/21 Página 2 - RTP2 A PhD student in Biomedicine at the University of Beira Interior is working on the development of a powder-based vaccine that is both preventive and therapeutic against COVID-19.
2020/11/20 - 2020/11/20 Grande Reportagem - TVI IMMUNITY - The Race Against COVID-19
2020/09/02 - 2020/09/02 Primeiro Jornal - SIC A PhD student in Biomedicine at the University of Beira Interior is working on the development of a powder vaccine that will be both preventive and therapeutic against COVID-19. The goal is to create a more effective and affordable vaccine.

Membro de comissão

Descrição da atividade
Tipo de participação 		Instituição / Organização
2021 - 2023 Doctoral Students Committee from the Health Sciences Research Centre of the University of Beira Interior (CAD-CICS-UBI)
Membro
 Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal

Tutoria

Tópico Nome do aluno
2023/04 - 2023/06 Formulate and characterize delivery systems for a DNA vaccine against COVID-19 Inês Bastos Pinheiro
2023/04 - 2023/06 Formulate and characterize delivery systems for a DNA vaccine against COVID-19 Rodrigo Miguel Ferreira Teixeira
2022/04 - 2022/06 Functionalized systems for targeted delivery of DNA vaccines to antigen-presenting cells Cinderela Ferreira Sezudo
2022/03 - 2022/05 Targeted delivery of a DNA vaccine against the COVID-19 to antigen-presenting cells Íris Maria Pereira Torres
2020/02 - 2020/06 Obtaining a minicircle DNA vector: Formulation and characterization of functionalized delivery systems Diana Filipa Cacheira Nunes
2020/02 - 2020/06 Obtaining a minicircle DNA vaccine: Purification by size exclusion chromatography Sofia Martins Gomes
Distinções

Prémio

2020 5th place of the contest 3 minutos, 1 slide .... A Tua tese!
Universidade da Beira Interior Instituto Coordenador da Investigação, Portugal

Banco Santander Portugal, Portugal