???global.info.a_carregar???
In 2021, I concluded my BSc in Biotechnology at the University of Aveiro (2018/2021) with a final grade of 17/20 (A in ECTS considering the prior four years). In the last year of my BSc, I did an internship as a research trainee with Dr. Ana Tavares and Dr. Miguel Neto at CICECO - Aveiro Institute of Materials and at the Department of Engineering and Materials, to develop the project “Oxidative enzymes immobilization on functionalized diamond surfaces” (graded 18/20), exploring the very first steps of a boron-doped diamond-based enzymatic biosensor for phenolic compounds with potential applications in monitoring neurologic biomarkers. Next, I completed the MSc in Biochemistry and Biomedicine (2021/2024) at the Faculty of Sciences of the University of Lisbon with a final grade of 19/20. In my master’s project, I worked with Dr. Sandra Vaz at Instituto de Medicina Molecular, Faculty of Medicine of the University of Lisbon, in the project “Role of astrocytes in the endocannabinoid-mediated long-term depression in the medial prefrontal cortex (mPFC)“ (graded 20/20). Our aim was to unveil the astroglial CB1R role in the synaptic plasticity of mPFC and its translational value for depressive behavior. Briefly, we proposed a new mechanism behind long-term depression (LTD) maintenance where astrocytes respond to endocannabinoids by increasing their intracellular Ca2+ frequency and decreasing glutamate uptake, ultimately shaping LTD. This work resulted in two publications as contributing author: one already submitted for peer-reviewed publication in Nature Communications journal, in collaboration with the highly regarded Dr. Alfonso Araque (Nat. Commun, doi:10.3390/cells12030381); and another still under preparation reviewing the astrocyte’s contribution to neuroplastic alterations underlying depression highlighting the glutamatergic/endocannabinoid systems. During the last year, I presented this work in several science communications, including three international conferences (FENS Regional Meeting 2023; VII symposium of Portuguese Glial Network and AIMS Meeting 2023) and a national oral communication (53º Encontro da Sociedade Portuguesa de Farmacologia). Additionally, I had the opportunity to participate in several workshops, including the highly competitive “Synaptic function in epileptic disorders” workshop (EpiEpiNet) hosted by Sapienza University in Rome. I also did a Laboratory Animal Science Course for Function A (+D) (former FELASA Category B) CONGENTO to become fully independent on animal manipulation. Since 2023 I have been a member of Sociedade Portuguesa de Neurociências, Sociedade Portuguesa de Farmacologia, and Portuguese Glial Network. Currently, I am visiting Dr. Luisa Pinto’s lab, whose work focuses on novel therapeutics for depression with a link to neuro-glial plasticity. Here, I learned new concepts and techniques while having the opportunity to engage in the team's ongoing projects. Moreover, I was recentrly awarded with a PhD Studentship (2024.01292.BDANA) by FCT, to develop the project "AstroMap: Understanding Adult Astrogliogenesis in Health and Depression" under the supervision of Dr. Luisa Pinto, Dr. Sandra Vaz and Dr. Teresa Canedo. During this period, I was also able to participate in the organizing committee of the VIII symposium of the Portuguese Glial Network and to enroll in a science communication program (Scientist Returns to School) that promotes the communication of science to children through interactive workshops. To date, I already conducted two workshops in Escola Básica de Arões Santa-Cristina and in Escola Básica de Arões São-Romão where we playfully explored how the brain works.
Identificação

Identificação pessoal

Nome completo
Leandro José Rodrigues Freitas

Nomes de citação

  • Rodrigues-Freitas, Leandro
  • Rodrigues-Freitas, L.
  • Leandro R. Freitas

Identificadores de autor

Ciência ID
8113-FAB2-51B9
ORCID iD
0000-0002-4374-1816

Endereços de correio eletrónico

  • lfreitas@medicina.ulisboa.pt (Profissional)

Telefones

Telefone
  • 217999411 Ext.: 47126 (Profissional)

Moradas

  • Instituto de Farmacologia e Neurociências, Faculdade de Medicina da Universidade de Lisboa, Avenida Professor Egas Moniz, 1649-028, Lisboa, Lisboa, Portugal (Profissional)
  • Life and Health Sciences Research Institute (ICVS) School of Medicine, University of Minho, Campus de Gualtar, 4710-057, Gualtar, Braga, Portugal (Profissional)

Websites

Domínios de atuação

  • Ciências Médicas e da Saúde - Medicina Básica - Neurociências
  • Ciências Médicas e da Saúde - Biotecnologia Médica

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador independente (B2) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1) Utilizador proficiente (C1)
Formação
Grau Classificação
2021 - 2023
Concluído
Bioquimica e Biomedicina (Mestrado integrado)
Universidade de Lisboa, Portugal
"Role of astrocytes in the endocannabinoid-mediated long-term depression in the medial prefrontal cortex" (TESE/DISSERTAÇÃO)
19
2022/11/09 - 2022/11/21
Concluído
Course on Laboratory Animal Sciences (EU Functions A+D) (Outros)
Congento Consortium for Genetically Tractable Organisms, Portugal

Universidade de Lisboa Instituto de Medicina Molecular, Portugal
2018 - 2021
Concluído
Biotecnologia (Licenciatura)
Universidade de Aveiro, Portugal
"Immobilization of oxidative enzymes in functionalized diamond" (TESE/DISSERTAÇÃO)
17
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2023/11/26 - Atual Investigador visitante (Investigação) Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
Universidade do Minho, Escola de Medicina, Portugal
2022/09/11 - 2023/09/11 Investigador (Investigação) Universidade de Lisboa Instituto de Medicina Molecular, Portugal
Universidade de Lisboa Faculdade de Medicina, Portugal
2020/10/06 - 2021/07/02 Estagiário de Investigação (Investigação) Universidade de Aveiro CICECO, Portugal
Universidade de Aveiro, Portugal
Projetos

Bolsa

Designação Financiadores
2025 - 2029 AstroMap: Understanding Adult Astrogliogenesis in Health and Depression
2024.01292.BDANA
Bolseiro de Doutoramento
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
Produções

Publicações

Poster em conferência
  1. Gonçalves-Ribeiro, Joana; Rodrigues-Freitas, L.; Nanclares C; Costa-Pinto, Sara; Sebastião, AM; Araque, Alfonso; Vaz SH. Autor correspondente: Gonçalves-Ribeiro, Joana. "CB1R MODULATES GLUTAMATE TRANSPORTERS: SHAPING LTD". Trabalho apresentado em VIII Symposium Portuguese Glial Network, 2024.
  2. Bernardo L. Tavares; Rodrigues-Freitas, L.; R. Fernandes, Diogo; Ebinuma, Valeria; João C. F. Nunes; Neves, Márcia C.; Girão, Ana V.; Neto, Miguel Ângelo da Costa; Tavares, Ana P. M.. Autor correspondente: Bernardo L. Tavares. "Boron-doped diamond surface as a platform for the development of an enzymatic biosensor". Trabalho apresentado em XXVIII Encontro Nacional da Sociedade Portuguesa de Química, 2023.
  3. Rodrigues-Freitas, L.; Gonçalves-Ribeiro, Joana; Sebastião, AM; Vaz, Sandra H.. Autor correspondente: Rodrigues-Freitas, L.. "Astrocytic CB1R impact on Long-Term Depression in the medial Prefrontal Cortex". Trabalho apresentado em FENS REGIONAL MEETING 2023, 2023.
  4. Gonçalves-Ribeiro, Joana; Rodrigues-Freitas, L.; Savchak O; Costa-Pinto, Sara; Sebastian R; Lillo A; Navarro-Brugal G; et al. Autor correspondente: Gonçalves-Ribeiro, Joana. "Astroglial CB1R activity is dictated by adenosine receptors in the rodent mPFC". Trabalho apresentado em FENS REGIONAL MEETING 2023, 2023.
  5. Rodrigues-Freitas, L.; Gonçalves-Ribeiro, Joana; Sebastião, AM; Vaz, Sandra H.. Autor correspondente: Rodrigues-Freitas, L.. "The relevance of astroglial CB1R in the rodent mPFC synaptic plasticity". Trabalho apresentado em VII Symposium of the Portuguese Glial Network, 2023.
  6. Gonçalves-Ribeiro, Joana; Nanclares C; Costa-Pinto, Sara; Neuparth-Sottomayor, Mariana; Rodrigues-Freitas, L.; Gualdino M; Sebastião, AM; Araque, Alfonso; Vaz SH. Autor correspondente: Gonçalves-Ribeiro, Joana. "CB1R downregulates glutamate transporters activity in a astroglial Ca2+-manner in the rodent mPFC". Trabalho apresentado em Glial Portuguese Meeting VII Symposium, 2023.
  7. Rodrigues-Freitas, L.; Gonçalves-Ribeiro, Joana; Sebastião, AM; Vaz, Sandra H.. "Unveiling the role of astrocytes in the eCB Long-Term Depression in the rodent mPFC". Trabalho apresentado em AIMS Meeting 2023 Research Competition, 2023.
  8. Tavares, Bernardo L.; Rodrigues-Freitas, L.; R. Fernandes, Diogo; Ebinuma, Valeria; João C. F. Nunes; Neves, Márcia C.; Girão, Ana V.; Neto, Miguel Ângelo da Costa; Tavares, Ana P. M.. Autor correspondente: Tavares, Bernardo L.. "Development of an Enzymatic Biosensor Based on Boron-doped Diamond Surface". Trabalho apresentado em XXVI Encontro Galego-Portugués de Química, 2022.
  9. Tavares, Bernardo L.; Rodrigues-Freitas, L.; R. Fernandes, Diogo; Ebinuma, Valeria; João C. F. Nunes; Neves, Márcia C.; Girão, Ana V.; Neto, Miguel Ângelo da Costa; Tavares, Ana P. M.. Autor correspondente: Tavares, Bernardo L.. "Development of an Enzymatic Biosensor Based on Boron-doped Diamond Surface". Trabalho apresentado em CICECO Jornadas 22 – Hand in Hand with Society, 2022.
Pré-impressão
  1. Sandra Vaz; Joana Gonçalves-Ribeiro; Leandro Rodrigues-Freitas; Carmen Nanclares; Ana Sebastião; Alfonso Araque. "Modulation of Astroglial Glutamate Transporters by CB1 Receptor Shapes Endocannabinoid-Mediated Long-Term Depression in the Medial Prefrontal Cortex". 2024. https://doi.org/10.21203/rs.3.rs-4281560/v1.
    10.21203/rs.3.rs-4281560/v1
Tese / Dissertação
  1. "Role of astrocytes in the endocannabinoid-mediated long-term depression in the medial prefrontal cortex". Mestrado, Universidade de Lisboa Instituto de Medicina Molecular, 2024.
  2. "Oxidative enzymes immobilization on functionalized diamond surfaces". Licenciatura, Universidade de Aveiro CICECO, 2021.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2023/02/01 Role of astrocytes in the endocannabinoid-mediated long-term depression in the medial prefrontal cortex 53º Encontro da Sociedade Portuguesa de Farmacologia
Sociedade Portuguesa de Farmacologia (Coimbra, Portugal)
2022/01/16 Role of astrocytes in the endocannabinoid-mediated long-term depression in the mPFC Neurosciences Seminar
Instituto de Farmacologia e Neurociência, iMM, FMUL (Lisboa, Portugal)

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação)
Instituição / Organização
2024/04/05 - Atual 8th Symposium of the Portuguese Glial Network. This annual event covers topics from molecular determinants of glial identity and development, their physiological roles in controlling circuit function and, ultimately, behavior. Top european researchers were present, including Andrea Volterr (University of Lausanne), Gonçalo Castelo-Branco (Karolinska Institutet), Ana Paula Pêgo (INEB), Catarina Gomes (University of Coimbra), Diogo Trigo (University of Aveiro) and Olga Barca (CiMUS) (2024/04/05)
Simpósio (Membro da Comissão Organizadora)
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2023 - Atual Dynamic neuroscience workshops given to children in the following schools with the support of Município de Fafe: 2024/03/11 - Escola Básica de Arões Santa Cristina (Turma M34) Escola Básica de Arões São Romão (Turma ASR4) (2023)
Oficina (workshop) (Coorganizador)
Native Scientists, Portugal

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2023/08/05 - 2023/08/06 The Astrocytic Control of Brain Circuits and Behavior is an ISN Satellite Workshop of the ISN-ESN Meeting 2023. This satellite event will gather experts to discuss the contribution of the underlying astrocyte physiological mechanisms to this equation: molecular, morphological, metabolic, and functional heterogeneity; and the balance between focal and global calcium astrocyte activity. This diverse panel will also ensure a stimulating discussion based on various opinions and approaches to tackle the common goal of understanding astrocyte modu
Oficina (workshop)
ISN-ESN Meeting Satellite Workshop “Astrocytic Control of Brain Circuits and Behavior”
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2023/05/03 - 2023/05/05 The FRM 2023 celebrates in-person interaction and knowledge exchange with the highest academic and professional standards through an outstanding scientific programme reflecting the latest breakthroughs in neuroscience.
Conferência
FENS Regional Meeting 2023
Federation of European Neuroscience Societies, Bélgica

Algarve Biomedical Center Research Institute, Portugal
2023/05/02 - 2023/05/02 7th Symposium of the Portuguese Glial Network. This annual event covered topics from the latest on glial cell physiology, biology, signaling, and ultrastructure with the invited speakers Amanda Sierra (Achucarro Basque Center for Neuroscience and Department of Biochemistry and Molecular Biology), Ana Luísa Cardoso (University of Coimbra), Christa Rhiner (Champalimaud Research Program), Corrado Calì (Università degli Studi di Torino), Juliana Rosa (Hospital Nacional de Parapléjicos - IDISCAM, Spain) and Renzo Mancuso (Universiteit Antwerpen).
Simpósio
VII Symposium of the Portuguese Glial Network
Federation of European Neuroscience Societies, Bélgica
2023/04/16 - 2023/04/16 AIMS Meeting [Annual International (bio)Medical Students Meeting] is the largest biomedical conference in Europe organized entirely by medical students, gathering each year over a thousand participants from all the world.
Oficina (workshop)
Workshop on Paediatric Neurosurgery (Theoretical/Practical)
Universidade de Lisboa Faculdade de Medicina, Portugal
2023/04/13 - 2023/04/16 AIMS Meeting [Annual International (bio)Medical Students Meeting] is the largest biomedical conference in Europe organized entirely by medical students, gathering each year over a thousand participants from all the world. It is held annually in Lisboa, Portugal and is advocated by the Faculty of Medicine of the Lisbon Students’ Local Committee. AIMS Meeting highlights the importance of medical education for all students of health sciences through a diverse approach and is a platform to build bridges between biomedical students around the world.
Congresso
AIMS Meeting 2023 [Annual International (bio)Medical Students Meeting]
Universidade de Lisboa Faculdade de Medicina, Portugal
2023/04/15 - 2023/04/15 AIMS Meeting [Annual International (bio)Medical Students Meeting] is the largest biomedical conference in Europe organized entirely by medical students, gathering each year over a thousand participants from all the world.
Oficina (workshop)
Workshop on Palliative Care (Theoretical/Practical)
Universidade de Lisboa Faculdade de Medicina, Portugal
2023/03/07 - 2023/03/07 Workshop held by Rede Glial Portuguesa to explore common techniques to evaluate neuronal excitability and neurotransmitter responses; the relevance of electrophysiological recordings for glial cells and examples from papers to discussion.
Oficina (workshop)
Workshop on electrophysiology recordings, Rede Glial Portuguesa
Universidade de Lisboa Instituto de Medicina Molecular, Portugal
2022/11/25 - 2022/11/25 Este workshop da rede SAÚDE promove uma reflexão sobre perspetivas atuais e futuras associadas à investigação e adoção de terapias génicas.
Oficina (workshop)
Terapia Génica – O Presente e o Futuro
Universidade de Lisboa, Portugal
2022/10/18 - 2022/10/18 6th Symposium of the Portuguese Glial Network. This annual event covers topics from molecular determinants of glial identity and development, physiological roles in and behavior. Top european researchers were present, including Karine Loulier (Institut des Neurosciences de Montpellier), Alex Charlet (University of Strasbourg Institute for Advanced Study), Nathalie Rouach (Centre Interdisciplinaire de Recherche en Biologie), Mick Hastings (MRC Laboratory of Molecular Biology) and Klaus Armin Nave (Max Planck Institute for Multidisciplinary Sciences)
Simpósio
VI Symposium of the Portuguese Glial Network
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Federation of European Neuroscience Societies, Bélgica
2022/09/23 - 2022/09/24 To advance the scientific and technological innovation in epilepsy research, the EU-funded EpiEpiNet project brings together reputed neuroscientists under a common network. The project will promote joint grant applications and joint training of young scientists to contribute to the development of innovative therapies against refractory forms of epilepsy. Further dissemination and training activities will enhance the educational nature of the network and increase awareness of epilepsy among caregivers and patients.
Congresso
From genes, synapses, and circuits to pave the way for novel drugs and strategies
Universidade de Lisboa Instituto de Medicina Molecular, Portugal
2022/09/19 - 2022/09/20 EpiEpiNet brings together reputed neuroscientists that have in common the aim of understanding of the basic mechanisms of epileptogenesis and their impact in synaptic and brain circuitry dysregulation, and to contribute to the development of innovative therapies against refractory forms of epilepsy.
Oficina (workshop)
Synaptic function in epileptic disorders: from basic to clinical implications
Università degli Studi di Roma La Sapienza Dipartimento di Fisiologia e Farmacologia Vittorio Erspamer, Itália

Membro de associação

Nome da associação Tipo de participação
2023 - Atual Sociedade Portuguesa de Neurociências Membro
2023 - Atual Sociedade Portuguesa de Farmacologia Membro
2023 - Atual Rede Glial Portuguesa Membro

Tutoria

Tópico Nome do aluno
2019/09/19 - 2021/07/26 Student mentor Hugo Santos, Mariana Pinto, Rafael Mascarenhas, Alexandre Santiago
Distinções

Prémio

2024 Merit and Excellence Scholarship 2024, Município de Fafe
Município de Fafe, Portugal