Personal identification

Full name

David Rufino-Ramos

Citation names

• Rufino-Ramos, David

Author identifiers

Ciência ID

0014-6760-FB96

ORCID iD

0000-0002-5125-9667

Email addresses

• rufinoramosdavid@gmail.com (Professional)

Knowledge fields

• Natural sciences - Biological Sciences - Cell Biology
• Natural sciences - Biological Sciences - Molecular Biology
• Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology
• Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism
• Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions
• Medical and Health Sciences - Basic Medicine - Neurosciences

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Portuguese (Mother tongue)
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
Spanish; Castilian	Upper intermediate (B2)	Upper intermediate (B2)	Intermediate (B1)	Upper intermediate (B2)

	Degree	Classification
2017 - 2022/12/12 Concluded	Doutoramento em Ciência Farmacêuticas (Doutoramento) Universidade de Coimbra Faculdade de Farmácia, Portugal "Extracellular vesicles: from brain communication to therapeutic delivery" (THESIS/DISSERTATION)
2019/06/02 - 2019/06/07 Concluded	Extracellular Vesicles: From Biology to Biomedical Applications (Outros) (Postgraduate Certificate) Gesellschaft zur Förderung der Lebenswissenschaften Heidelberg GmbH, Germany
2017 - 2017 Concluded	FELASA Category B certified researcher for carrying out animal experiments (Outros) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2011 - 2016 Concluded	Mestrado Integrado em Ciências Farmacêuticas (Mestrado integrado) Universidade de Coimbra Faculdade de Farmácia, Portugal "CRISPR/CAS9 : uma ferramenta de edição genética para investigação e novas terapias" (THESIS/DISSERTATION)	16,3

Science

	Category Host institution	Employer
2023/05/08 - Current	Postdoc (Research)	Harvard Medical School, United States
	Harvard Medical School, United States (...)
2017/10/01 - 2022/09/30	Researcher (Research)	Fundação para a Ciência e a Tecnologia, Portugal
	CNC.IBILI, Portugal
2020/10 - 2021/04	Visiting Researcher (Research)	Fundação para a Ciência e a Tecnologia, Portugal
	Harvard Medical School, United States (...)
2016/09 - 2017/09	Researcher (Research)	Fundação para a Ciência e a Tecnologia, Portugal
	CNC.IBILI, Portugal

Contract

	Designation	Funders
2017 - Current	EU Joint Programme - Neurodegenerative Disease Research (2013 JPND Transnational call) project SynSpread JPND-CD/0001/2013	Ongoing
2024/03/01 - 2026/02/28	Developing and delivering of novel gene editing technologies to contract GAA repeats in Friedreich's ataxia NA Principal investigator Friedreich's Ataxia Research Alliance (FARA), United States	Friedreich's Ataxia Research Alliance (FARA) Ongoing
2017/10/01 - 2021/11/30	Exosome­-based silencing of Machado-­Joseph disease  SFRH/BD/132618/2017 Universidade de Coimbra, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2018/07/01 - 2021/06/30	Bloqueio da neurodegenerescência por dispersão de silenciadores génicos PTDC/BTM-SAL/29716/2017 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2016/05/01 - 2020/03/31	Iniciativa Europeia para a doença de Machado-Joseph / Ataxia Espinocerebelosa do tipo 3 JPCOFUND/0001/2015 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2015/03/01 - 2018/12/31	Papel e mecanismos da propagação da sinucleina e da ataxina-3 nas doenças de Parkinson e Machado-Joseph JPND-CD/0001/2013 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Other

	Designation	Funders
2020 - Current	Covid-19 Lab @ CNC / ViraVector NA Researcher Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal	Ongoing
2020 - Current	COVID-19 UC Lab NA Researcher Universidade de Coimbra Faculdade de Farmácia, Portugal

Publications

Conference poster	"Extracellular Vesicles carrying engineered miRNAs alleviate Machado- Joseph Disease (MJD)". Paper presented in BEB Day 2022: The overview effect, 2022. "Using genetically modified extracellular vesicles as a non-invasive strategy to evaluate brain cargo". Paper presented in BEB Day 2022: The overview effect, 2022. "Intranasal delivery of Extracellular Vesicles carrying silencing sequences alleviates Machado-Joseph Disease". Paper presented in XVII Meeting of the Portuguese Society for Neuroscience, 2021. Rufino-Ramos, David. "An effective packaging cell line to highly produce Extracellular Vesicles for therapy of Machado-Joseph Disease". Paper presented in MicroBiotec 19, 5 - 7 December 2019, Coimbra, Portugal, 2019. Rufino-Ramos, David. "Extracellular Vesicles: a novel promising tool for therapy of Machado-Joseph Disease". Paper presented in 1st Annual Meeting in Pharmaceutical Sciences, 15 November 2019, Coimbra, Portugal, 2019. Rufino-Ramos, David. "Extracellular vesicles for therapy of spinocerebellar ataxia type 3". Paper presented in ESGCT 27th Annual, 22 - 25 October 2019, Barcelona, Spain, 2019. Rufino-Ramos, David. "Extracellular vesicles as a deliver system of miRNAs in Spinocerebellar ataxia type 3". Paper presented in EMBO PRACTICAL COURSE: Extracellular Vesicles: From Biology to Biomedical Applications, 2 - 7 Jun 2019, EMBL Heidelberg, Germany, 2019. Rufino-Ramos, David. "miRNA delivery via Exosomes in Machado-Joseph Disease". Paper presented in VIII National RNA Meeting: ptRNA2019, 2019. Rufino-Ramos, David. "EVs: a promising tool to deliver miRNAs against Machado-Joseph Disease". Paper presented in BBEV2018-The Biomarkers and Biogenesis of Extracellular Vesicles Workshop, 2018. Rufino-Ramos, David. "Exosomal isolation: towards an improved protocol". Paper presented in III ENEBT, 3rd-5th March 2017, University of Aveiro, Portugal, 2017.
Journal article	Rufino-Ramos, David; Leandro, Kevin; Perdigão, Pedro R.L.; O'Brien, Killian; Pinto, Maria Manuel; Santana, Magda M.; van Solinge, Thomas S.; et al. Corresponding author: Breyne, Koen. "Extracellular communication between brain cells through functional transfer of Cre mRNA mediated by extracellular vesicles". Molecular Therapy 31 7 (2023): 2220-2239. http://dx.doi.org/10.1016/j.ymthe.2023.05.012. 10.1016/j.ymthe.2023.05.012 Rufino-Ramos, David; Albuquerque, Patrícia R.; Leandro, Kevin; Carmona, Vitor; Martins, Inês M.; Fernandes, Rita; Henriques, Carina; et al. "Extracellular vesicle-based delivery of silencing sequences for the treatment of Machado-Joseph disease/spinocerebellar ataxia type 3". Molecular Therapy 31 5 (2023): 1275-1292. http://dx.doi.org/10.1016/j.ymthe.2023.04.001. Published • 10.1016/j.ymthe.2023.04.001 Breyne, Koen; Ughetto, Stefano; Rufino-Ramos, David; Mahjoum, Shadi; Grandell, Emily A.; de Almeida, Luís P.; Breakefield, Xandra O.. "Exogenous loading of extracellular vesicles, virus-like particles, and lentiviral vectors with supercharged proteins". Communications Biology 5 1 (2022): http://dx.doi.org/10.1038/s42003-022-03440-7. 10.1038/s42003-022-03440-7 Rufino-Ramos, David; Lule, Sevda; Mahjoum, Shadi; Ughetto, Stefano; Cristopher Bragg, D.; Pereira de Almeida, Luís; Breakefield, Xandra O.; Breyne, Koen. "Using genetically modified extracellular vesicles as a non-invasive strategy to evaluate brain-specific cargo". Biomaterials 281 (2022): 121366. http://dx.doi.org/10.1016/j.biomaterials.2022.121366. 10.1016/j.biomaterials.2022.121366 Mahjoum, Shadi; Rufino-Ramos, David; Almeida, Luís Pereira de; Broekman, Marike L. D.; Breakefield, Xandra O.; van Solinge, Thomas S.. "Living Proof of Activity of Extracellular Vesicles in the Central Nervous System". (2021): http://hdl.handle.net/10316/95919. 10.3390/ijms22147294 Barros, Inês; Marcelo, Adriana; Silva, Teresa P.; Barata, João; Rufino-Ramos, David; Pereira de Almeida, Luís; Miranda, Catarina O.. "Mesenchymal Stromal Cells’ Therapy for Polyglutamine Disorders: Where Do We Stand and Where Should We Go?". Frontiers in Cellular Neuroscience 14 (2020): http://dx.doi.org/10.3389/fncel.2020.584277. 10.3389/fncel.2020.584277 Rufino-Ramos, António. "Extracellular vesicles: Novel promising delivery systems for therapy of brain diseases". Extracellular vesicles: Novel promising delivery systems for therapy of brain diseases (2017): http://www.sciencedirect.com/science/article/pii/S0168365917306971.
Newspaper article	Rufino-Ramos, David. "Vinhas, Ana Catarina; Vasconcelos-Ferreira, Ana; Henriques, Carina S.; Silva, Ana Carolina; Miranda, Catarina Oliveira; Henriques, Daniel; Oliveira, Daniela; et al. "Uma viagem rara". PÚBLICO, 2020/02/28. https://www.publico.pt/2020/02/28/ciencia/fotogaleria/viagem-rara-400481.", 2020
Preprint	Rufino-Ramos, David; Leandro, Kevin; Perdigão, Pedro R.L.; O’Brien, Killian; Pinto, Maria Manuel; Santana, Magda M.; van Solinge, Thomas S; et al. "Extracellular communication between brain cells through functional transfer of Cre mRNA". 2023. http://dx.doi.org/10.1101/2023.01.29.525937. 10.1101/2023.01.29.525937 da Silva, Joana Ferreira; Tou, Connor J.; King, Emily M.; Eller, Madeline L.; Ma, Linyuan; Rufino-Ramos, David; Kleinstiver, Benjamin P.. "Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases". 2023. http://dx.doi.org/10.1101/2023.09.12.557440. 10.1101/2023.09.12.557440
Thesis / Dissertation	"Extracellular vesicles: from brain communication to therapeutic delivery". PhD, Universidade de Coimbra, 2022. Ramos, António David Rufino. "Relatório de estágio curricular em farmácia comunitária". Master, 2016. http://hdl.handle.net/10316/42063.

Artistic / Performance

Theatre play

2020. Viegas, Teresa Ana; Marques, Andreia; Santos, Bárbara; Henriques, Carina S.; Rufino-Ramos, David; Lobo, Diana Duarte; Alfaiate, Inês; et al. 2019. Holy CIBB..

Other

Other output

CRISPR/CAS9 : uma ferramenta de edição genética para investigação e novas terapias. Monografia realizada no âmbito da unidade Estágio Curricular do Mestrado Integrado em Ciências Farmacêuticas, apresentada à Faculdade de Farmácia da Universidade de Coimbra. Os crescentes avanços na área da terapia génica têm revolucionado a maneira de pensar sobre tudo o que nos rodeia, particularmente na área da saúde. O sistema CRISPR/Cas9 (clustered regularly short palindromic repeats-CRISPR a. 2016. Almeida, Luís Pereira de. http://hdl.handle.net/10316/42065.

Award

2022	1st Prize at "InnoCore Challenge Initiative"
2022	ISEV2022 Young Investigator/Early Career Research Scholarship
2022	27th ESACT Meeting Young Investigator Scholarship
2021	FLAD PhD Mobility Scholarship Fundação Luso-Americana, Portugal
2020	1º Prize at "Building Tomorrow Together" - Acceleration and Open Innovation Competition promoted by Roche Portugal
2019	EMBO Practical Course: Extracellular Vesicles: From Biology to Biomedical Applications - Young Investigator Scholarship Boehringer Ingelheim Fonds, Germany
2019	VIIPtRNA2019 meeting - Young Investigator Scholarship Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017	Poster award in III ENEBT Universidade de Aveiro, Portugal
2017	PhD Scholarship - SFRH/BD/132618/2017 Fundação para a Ciência e a Tecnologia, Portugal
2016	Prémio 3% dos Melhores Estudantes Universidade de Coimbra, Portugal

Other distinction

2024	Postdoctoral Fellowship Friedreich's Ataxia Research Alliance (FARA), United States