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Maria Daniela Silva (MDS) obtained her master's degree in Biomedical Engineering from the University of Minho (UM) in October 2016. From December 2016 to December 2017, she was a research fellow at the Centre of Biological Engineering (CEB), UM, Braga. MDS was then awarded a studentship from Fundação para a Ciência e Tecnologia (FCT) to perform her PhD at the same institution. During her PhD, she had the opportunity to develop part of her work at the International Iberian Nanotechnology Laboratory (INL), Braga. Also, in 2021, she performed a 6-month visiting period at the Massachusetts Eye and Ear Hospital and the Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, USA. In July 2022, MDS completed her PhD degree in Biomedical Engineering from the University of Minho. She worked as a research fellow and then as a post-doc researcher in an FCT-funded project (DeathTrigger - PTDC/BIA-MIC/2312/2020). MDS has published 14 peer-reviewed papers in international journals, of which 9 as first-author (2 of them with a co-authorship), and 2 book chapters (h index = 8). Her work has been published in high quality journals, including mSystems, International Journal of Pharmaceutics, ACS Infectious Diseases, FEMS Microbiology Reviews and Critical Reviews in Microbiology. MDS has also authored several communications, being the presenting author of 4 oral communications and 7 posters in international conferences. Additionally, she is a reviewer for international peer-reviewed journals. MDS has been an instructor in various editions of the Hands-on Phage Biotechnology Course and was a staff member of the 'Viruses of Microbes 2022' international conference. Her involvement extends to outreach activities, including the CEB open day, workshops, and exhibitions. She co-supervised a master's student and mentored other two students. MDS is a member of the COST action CA21145 "European Network for Diagnosis and Treatment of Antibiotic-resistant Bacterial Infections", the Microbiology Society, and the Spanish Portuguese Local Chapter of the Controlled Release Society (SPLC-CRS).
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Identification

Personal identification

Full name
Maria Daniela Ferreira da Silva

Citation names

  • Silva, Maria Daniela

Author identifiers

Ciência ID
0018-D3A5-4674
ORCID iD
0000-0003-2495-6026
Scopus Author Id
57192295120

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Microbiology
  • Natural sciences - Biological Sciences - Virology
  • Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology
  • Engineering and Technology - Medical Engineering - Medical Engineering
  • Engineering and Technology - Nano-technology - Nano-materials

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Upper intermediate (B2) Advanced (C1) Advanced (C1) Advanced (C1)
French Intermediate (B1) Intermediate (B1) Elementary (A2) Intermediate (B1)
Education
Degree Classification
2017/10 - 2022/07
Concluded
 Biomedical Engineering (Doutoramento)
Universidade do Minho, Portugal
"Development of a new approach to control otitis media pathogens" (THESIS/DISSERTATION)
 Approved unanimously with the highest degree of very good
2011/09 - 2016/10
Concluded
 Engenharia Biomédica (Mestrado integrado)
Major in Engenharia Clínica
Universidade do Minho, Portugal
"Novel strategies to control periodontal disease" (THESIS/DISSERTATION)
 16 out of 20
Affiliation

Others

Category
Host institution 		Employer
2023/01/03 - 2024/08/31 Post-doc researcher Universidade do Minho Centro de Engenharia Biológica, Portugal
2022/06/01 - 2022/11/30 Research fellow Universidade do Minho Centro de Engenharia Biológica, Portugal
2019/09/16 - 2022/05/31 PhD student (Associate) International Iberian Nanotechnology Laboratory, Portugal
2018/01/01 - 2022/05/31 PhD student fellow Universidade do Minho Centro de Engenharia Biológica, Portugal
2021/05/28 - 2021/11/16 Visiting PhD student Massachussets Eye and Ear, United States
2021/05/28 - 2021/11/16 Visiting PhD student Harvard University Wyss Institute for Biologically Inspired Engineering, United States
2016/12/27 - 2017/12/26 Research fellow Universidade do Minho Centro de Engenharia Biológica, Portugal
2015/09/01 - 2016/10/21 Master's student Universidade do Minho Centro de Engenharia Biológica, Portugal
Projects

Grant

Designation Funders
2018/01/01 - 2022/05/31 Development of a new approach to control otitis media pathogens
SFRH/BD/128825/2017
COVID/BD/152363/2022
PhD Student Fellow
Universidade do Minho Centro de Engenharia Biológica, Portugal

International Iberian Nanotechnology Laboratory, Portugal

Massachusetts Eye and Ear Eaton-Peabody Laboratories, United States

Harvard University Wyss Institute for Biologically Inspired Engineering, United States
Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2022/06/01 - 2024/08/31 DeathTrigger - Activating Dormant Bacteria with phage-derived proteins to enhance antibiotic efficacy
PTDC/BIA-MIC/2312/2020
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2016/12/27 - 2017/12/26 Phages to control foodborne pathogens
UID/BIO/04469/2013
Research Fellow
Universidade do Minho Centro de Engenharia Biológica, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Silva, Maria Daniela; Melo, Luís D. R.. "Phage-Host Interaction Analysis Using Flow Cytometry". 2024.
    10.1007/978-1-0716-3523-0_8
  2. Milho, Catarina; Silva, Maria Daniela; Sillankorva, Sanna; Harper, David R.. "Biofilm Applications of Bacteriophages". In Bacteriophages, 789-822. Springer International Publishing, 2021.
    10.1007/978-3-319-41986-2_27
Conference poster
  1. Silva, Maria Daniela; Pinto, Graça; França, Ângela; Azeredo, Joana; Melo, Luís D. R.. "Comparative RNA-Seq analysis of S. epidermidis under SEP1 phage infection during exponential and stationary growth". Paper presented in Encontro Ciência 2024, 2024.
  2. Silva, Maria Daniela; Paris, Juan L.; Ray, Kaelin; Gama, Miguel; Silva, Bruno F. B.; Sillankorva, Sanna. "Exploring endolysin-loaded liposomes for a transtympanic treatment of S. pneumoniae otitis media". Paper presented in Viruses of Microbes 2022, 2022.
  3. Silva, Maria Daniela; Ray, Kaelin; Gama, Miguel; Remenschneider, Aaron K.; Sillankorva, Sanna. "Transtympanic delivery of the pneumococcal endolysin MSlys via PEGylated liposomes". Paper presented in XIV Spanish-Portuguese Conference on Controlled Drug Delivery, 2022.
  4. Silva, Maria Daniela; Oliveira, Hugo; Faustino, Alberta; Sillankorva, Sanna. "Characterization and antipneumococcal efficacy of MSlys endolysin". Paper presented in Phage Futures Congress 2021, 2021.
  5. Silva, Maria Daniela; Paris, Juan L.; Gama, Miguel; Silva, Bruno F. B.; Sillankorva, Sanna. "Endolysin-loaded liposomes for treatment of S. pneumoniae otitis media". Paper presented in 3rd SPCL-CRS Young Scientists Virtual Meeting, 2021.
  6. Silva, Maria Daniela; Sillankorva, Sanna. "Seeking a phage-based therapy to control otitis media". Paper presented in 23rd Biennial Evergreen International Phage Meeting, 2019.
  7. Milho, Catarina; Silva, Maria Daniela; Alves, Diana F. ; Azeredo, Joana; Sillankorva, Sanna. "Interspecies interactions of Salmonella Enteritidis and Escherichia coli dual-species biofilms and their control by bacteriophages". Paper presented in Viruses of Microbes 2018 (EMBO Workshop), 2018.
  8. Milho, Catarina; Silva, Maria Daniela; Alves, Diana F. ; Azeredo, Joana; Sillankorva, Sanna. "Interspecies interactions and bacteriophage control in S. Enteritidis and E. coli mixed biofilms". Paper presented in Microbiotec'17 - Congress of Microbiology and Biotechnology 2017, 2017.
  9. Silva, Maria Daniela; Pinto, Graça; Azeredo, Joana; Sillankorva, Sanna. "Control of Porphyromonas gingivalis using a bacterial-derived peptidoglycan hydrolase". Paper presented in Microbiotec'17 - Congress of Microbiology and Biotechnology 2017, 2017.
  10. Oliveira, Ana; Ribeiro, Henrique; Silva, Ana Catarina; Silva, Maria Daniela; Sousa, Jéssica ; Rodrigues, Célia F.; Melo, Luís D. R.; Sillankorva, Sanna. Corresponding author: Sillankorva, Sanna. "Antimicrobial interaction of honey and phage against E. coli biofilms". Paper presented in PHAGES 2017 - Bacteriophages in Medicine, Food and Biotechnology, 2017.
  11. Milho, Catarina; Silva, Maria Daniela; Melo, Luís D. R.; Santos, Sílvio B.; Joana Azeredo; Sillankorva, Sanna. "Control of Salmonella Enteritidis on food contact surfaces with bacteriophage PVP-SE2". Paper presented in PHAGES 2017 - Bacteriophages in Medicine, Food and Biotechnology, 2017.
Journal article
  1. Lopes, Maria Sequeira; Silva, Maria Daniela; Azeredo, Joana; Melo, Luís D.R.. "Coagulase-Negative Staphylococci phages panorama: Genomic diversity and in vitro studies for a therapeutic use". Microbiological Research 290 (2025): 127944. http://dx.doi.org/10.1016/j.micres.2024.127944.
    10.1016/j.micres.2024.127944
  2. Silva, Maria Daniela; Pinto, Graça; França, Angela; Azeredo, Joana; Melo, Luís D. R.. "Phage SEP1 hijacks Staphylococcus epidermidis stationary cells’ metabolism to replicate". mSystems 9 7 (2024): http://dx.doi.org/10.1128/msystems.00263-24.
    10.1128/msystems.00263-24
  3. Vinayakumar, K. B.; Silva, Maria Daniela; Martins, Artur; Mundy, Stephen; González-Losada, Pedro; Sillankorva, Sanna. "Levofloxacin-Loaded Microneedles Produced Using 3D-Printed Molds for Klebsiella Pneumoniae Biofilm Control". Advanced Therapeutics (2023): http://dx.doi.org/10.1002/adtp.202200320.
    10.1002/adtp.202200320
  4. Silva, Maria Daniela; André, Camille; Bispo, Paulo J.M.. "Targeted Killing of Ocular Streptococcus pneumoniae by the Phage Endolysin MSlys". Ophthalmology Science 2 4 (2022): 100193. http://dx.doi.org/10.1016/j.xops.2022.100193.
    10.1016/j.xops.2022.100193
  5. Silva, Maria Daniela; Ray, Kaelin; Gama, Miguel; Remenschneider, Aaron K.; Sillankorva, Sanna. "Ex vivo transtympanic permeation of the liposome encapsulated S. pneumoniae endolysin MSlys". International Journal of Pharmaceutics 620 (2022): 121752. http://dx.doi.org/10.1016/j.ijpharm.2022.121752.
    10.1016/j.ijpharm.2022.121752
  6. Silva, Maria Daniela; Lima, António; Marçal, Nuno; Dias, Luís; Gama, Miguel; Sillankorva, Sanna. "Identification of the Bacterial Pathogens in Children with Otitis Media: A Study in the Northwestern Portuguese District of Braga". Microorganisms 10 1 (2021): 54. http://dx.doi.org/10.3390/microorganisms10010054.
    10.3390/microorganisms10010054
  7. Silva, Maria Daniela; Paris, Juan L.; Gama, Francisco Miguel; Silva, Bruno F. B.; Sillankorva, Sanna. "Sustained Release of a Streptococcus pneumoniae Endolysin from Liposomes for Potential Otitis Media Treatment". ACS Infectious Diseases 7 8 (2021): 2127-2137. http://dx.doi.org/10.1021/acsinfecdis.1c00108.
    10.1021/acsinfecdis.1c00108
  8. Pinto, Ana Mafalda; Silva, Maria Daniela; Pastrana, Lorenzo M; Bañobre-López, Manuel; Sillankorva, Sanna. "The clinical path to deliver encapsulated phages and lysins". FEMS Microbiology Reviews 45 5 (2021): http://dx.doi.org/10.1093/femsre/fuab019.
    10.1093/femsre/fuab019
  9. Silva, Maria Daniela; Oliveira, Hugo; Faustino, Alberta; Sillankorva, Sanna. "Characterization of MSlys, the endolysin of Streptococcus pneumoniae phage MS1". Biotechnology Reports 28 (2020): e00547. http://dx.doi.org/10.1016/j.btre.2020.e00547.
    10.1016/j.btre.2020.e00547
  10. Milho, Catarina; Silva, Maria Daniela; Alves, Diana; Oliveira, Hugo; Sousa, Clara; Pastrana, Lorenzo M.; Azeredo, Joana; Sillankorva, Sanna. "Escherichia coli and Salmonella Enteritidis dual-species biofilms: interspecies interactions and antibiofilm efficacy of phages". Scientific Reports 9 1 (2019): https://doi.org/10.1038/s41598-019-54847-y.
    10.1038/s41598-019-54847-y
  11. Silva, Maria Daniela; Sillankorva, Sanna. "Otitis media pathogens - A life entrapped in biofilm communities". Critical Reviews in Microbiology 45 5-6 (2019): 595-612. https://doi.org/10.1080/1040841X.2019.1660616.
    10.1080/1040841x.2019.1660616
  12. Milho, Catarina; Silva, Maria Daniela; Melo, Luís; Santos, Sílvio; Azeredo, Joana; Sillankorva, Sanna. "Control of Salmonella Enteritidis on food contact surfaces with bacteriophage PVP-SE2". Biofouling 34 7 (2018): 753-768. https://doi.org/10.1080/08927014.2018.1501475.
    10.1080/08927014.2018.1501475
  13. Oliveira, Ana; Ribeiro, Henrique G.; Silva, Ana C.; Silva, Maria D.; Sousa, Jessica C.; Rodrigues, Célia F.; Melo, Luís D. R.; Henriques, Ana F.; Sillankorva, Sanna. "Synergistic Antimicrobial Interaction between Honey and Phage against Escherichia coli Biofilms". Frontiers in Microbiology 8 (2017): https://doi.org/10.3389/fmicb.2017.02407.
    10.3389/fmicb.2017.02407
  14. Pinto, Graça; Silva, Maria Daniela; Peddey, Mark; Sillankorva, Sanna; Azeredo, Joana. "The role of bacteriophages in periodontal health and disease". Future Microbiology 11 10 (2016): 1359-1369. https://doi.org/10.2217/fmb-2016-0081.
    10.2217/fmb-2016-0081
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/04/29 Staphylococcus epidermidis phage activates metabolic and biosynthetic processes in stationary cells ESCMID Global 2024 - 34th Congress of the European Society of Clinical Microbiology and Infectious Diseases
(Barcelona, Spain)
2024/04/09 Phage SEP1 activates and replicates efficiently in S. epidermidis stationary phase cells Microbiology Society Annual Conference
(Edinburgh, United Kingdom)
2024/04/05 Phage-host interaction with cells in different metabolic states: A S. epidermidis case XII Congress of Microbiologists of Serbia
(Belgrade, Serbia)
2024/03/22 Bacteriophages effective against stationary cells: promising agents against antibiotic tolerant bacteria EURESTOP (European Network for diagnosis and treatment of antibiotic-resistant bacterial infections – COST Action CA21145) General Meeting
(Rome, Italy)
2024/02/19 Bacteriófagos e suas proteínas no controlo de infeções Outreach event: "O CEB vai à Escola" - Escola Básica de Braga Oeste
(Braga, Portugal)
2023/12/08 The unique ability of Staphylococcus epidermidis phage SEP1 activating dormant cells Microbiotec 23
(Covilhã, Portugal)
2023/10/02 Exploitation of bacteriophage SEP1 interaction with Staphylococcus epidermidis 9142 exponential and stationary cells: a transcriptomic analysis International Symposium of the DFG-Priority Program SPP 2330 “New Concepts in Prokaryotic Virus-host Interactions – From Single Cells to Microbial Communities
(Berlin, Germany)
2022/07/25 Towards a transtympanic treatment of pneumococcal otitis media using endolysin-loaded liposomes Controlled Release Society 2021 Virtual Annual Meeting
2022/04/28 Liposomes loaded with the pneumococcal endolysin MSlys: From in vitro characterization to ex vivo permeation across the tympanic membrane 8th Galenus International Workshop: “The shape of things to come - Drug delivery systems from head to toe”
(Valencia, Spain)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2023/09 - 2024/12 Characterization of Staphylococcus aureus phages active against dormant cells
Co-supervisor of Beatriz Silva Matos
 Biomedical Engineering (Master)
Universidade do Minho, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2024/05/24 - 2024/05/24 Exhibitor on "Feira Internacional Curtir Ciência FIC^2" (Outreach event) (2024/05/24 - 2024/05/25)
Exhibition (Other)
 Universidade do Minho, Portugal
2024/04/19 - 2024/04/19 Instructor of the workshop "Viruses that kill bacteria" at "UPA - UMinho de Portas Abertas" (2024/04/18 - 2024/04/20)
Workshop (Other)
 Universidade do Minho, Portugal
2023/06/19 - 2023/06/23 Staff member and instructor of the practical session "Monitoring bacteriophage/host interaction" of the 5th International Hands-on Phage Biotechnology course (2023/06/19 - 2023/06/23)
Workshop (Other)
 Universidade do Minho Centro de Engenharia Biológica, Portugal
2022/11/25 - 2022/11/25 Instructor of the session "Viruses that treat diseases: where to find them and how to use them" at CEB open day (Outreach event) (2022/11/25 - 2022/11/25)
Exhibition (Other)
 Universidade do Minho Centro de Engenharia Biológica, Portugal
2022/07/18 - 2022/07/22 Staff member of the "Viruses of Microbes 2022" international conference (2022/07/18 - 2022/07/22)
Conference (Other)
 Universidade do Minho Centro de Engenharia Biológica, Portugal
2019/06/17 - 2019/06/21 Staff member and instructor of the practical session "Isolation and characterization of bacteriophages" of the 4th International Hands-on Phage Biotechnology course (2019/06/17 - 2019/06/21)
Workshop (Other)
 Universidade do Minho Centro de Engenharia Biológica, Portugal
2018/11/24 - 2018/11/24 Instructor of the session "What bugs can you find in your ears when you're sick?" at CEB open day (Outreach event) (2018/11/24 - 2018/11/24)
Exhibition (Other)
 Universidade do Minho Centro de Engenharia Biológica, Portugal
2017/06/19 - 2017/06/21 Staff member and instructor of the practical session "Isolation and characterization of bacteriophages" of the 3rd International Hands-on Phage Biotechnology course (2017/06/19 - 2017/06/21)
Workshop (Other)
 Universidade do Minho Centro de Engenharia Biológica, Portugal
2014/12/17 - 2014/12/19 X Jornadas de Engenharia Biomédica (2014/12/17 - 2014/12/19)
Symposium (Member of the Organising Committee)
 Universidade do Minho, Portugal
2013/02/18 - 2013/02/19 VIII Jornadas de Engenharia Biomédica (2013/02/18 - 2013/02/19)
Symposium (Member of the Organising Committee)
 Universidade do Minho, Portugal

Ad Hoc journal article review

Journal title (ISSN) Publisher
2024/01 - Current Virology Journal Springer Nature
2023/12 - Current International Journal of Molecular Sciences MDPI
2023/11 - Current Scientific Reports Nature Publishing Group
2022/12/09 - Current Microorganisms MDPI
2022/10/17 - Current Biology (2079-7737) MDPI
2021/01 - Current Viruses (1999-4915) MDPI

Association member

Society Organization name Role
2024/01 - Current Microbiology Society Member
2023/02 - Current CA21145 - European Network for diagnosis and treatment of antibiotic-resistant bacterial infections (EURESTOP) Member
2021/01 - Current Spanish Portuguese Local Chapter of the Controlled Release Society (SPLC-CRS) Member
2014/02 - 2015/03 Gabinete de Alunos de Engenharia Biomédica da Universidade do Minho Vice-president of the Direction
2012/06 - 2013/03 Gabinete de Alunos de Engenharia Biomédica da Universidade do Minho Vice-president of the Audit Committee

Mentoring / Tutoring

Topic Student name
2023/08 - 2023/10 Intern under the COST action CA21145 (European Network for diagnosis and treatment of antibiotic-resistant bacterial infections) Semra Tasdurmazli
2021/06/01 - 2021/08/13 Harvard Global Health Institute Summer Research Fellow Kaelin Ray