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Gabriel António Amaro Monteiro. Completed the Doutoramento in Biotecnologia in 1998 by Universidade de Lisboa Instituto Superior Técnico, Mestrado in Biotecnologia in 1991 by Universidade de Lisboa Instituto Superior Tecnico and Licenciatura in Biologia in 1988 by Universidade de Coimbra Faculdade de Ciencias e Tecnologia. Is Associate Professor in Universidade de Lisboa Instituto Superior Tecnico. Published 122 articles in journals. Has 11 section(s) of books. Supervised 10 PhD thesis(es) e co-supervised 7. Supervised 22 MSc dissertation(s) e co-supervised 31. Supervised 1 work(s) of course completion of LSc/BSc. Has received 1 awards and/or honors. Participates and/or participated as Principal investigator in 7 project(s) and Researcher in 23 project(s). Works in the area(s) of Engineering and Technology with emphasis on Industrial Biotechnology. In their professional activities interacted with 286 collaborator(s) co-authorship of scientific papers. In his curriculum Ciência Vitae the most frequent terms in the context of scientific, technological and artistic-cultural output are: DNA vaccines; Escherichia coli; plasmid; minicircle; gene therapy; Bactérias ácido lácticas; DNA plasmídico; Biologia sintética; Engenharia de estirpes; Biofilmes; Proteínas recombinantes; Celulose; Módulos de Ligação a Carbohidratos; Reconhecimento Biomolecular; Materiais Funcionais Avançados; Vírus Influenza aviário; Vacinas de DNA; Sequências de direccionamento; DIVA; Plasmídeo; Entrega de fármacos; Endereçamento sub-celular; Tráfego intracelular; Biologia de sistemas; Agentes terapêuticos; Cancro; Lab-on-Chip; fluorescência 2D; biosensores; fotosensores de silício amorfo; Gripe Aviária; Influenzavirus A; Genética Reversa; microarrays; biochips; DNA; hibridização; Tuberculose multiresistente; Mycobacterium tuberculosis; Bombas de efluxo; Novos anti-bacilares; protótipos de vacinas de DNA; tripanossomose africana animal; resposta imunológica; direccionamento de antigénios; barreira de nucleases; vectores plasmidícos; tráfego plasmídico; terapia génica; Biochemistry Engineering and Biotechnology; Chemistry; .
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Identification

Personal identification

Full name
Gabriel António Amaro Monteiro

Citation names

  • Monteiro, Gabriel António Amaro

Author identifiers

Ciência ID
0213-4B36-CE79
ORCID iD
0000-0003-4840-6299

Email addresses

  • gabmonteiro@tecnico.ulisboa.pt (Professional)

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions
  • Engineering and Technology - Industrial Biotechnology
Education
Degree Classification
1998
Concluded
 Biotecnologia (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Mecanismos Moleculares Envolvidos na Activação da H+-atpase da Membrana Plasmática de Saccharomyces Cerevisiae pelo Etanol" (THESIS/DISSERTATION)
1991
Concluded
 Biotecnologia (Mestrado)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
 Muito Bom (média de 17 valores na parte escolar)
1988
Concluded
 Biologia (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
 15 valores
Affiliation

Teaching in Higher Education

Category
Host institution 		Employer
2016/01/01 - Current Associate Professor (University Teacher) Universidade de Lisboa Instituto Superior Tecnico, Portugal
2000/10/01 - 2015/12/01 Assistant Professor (University Teacher) Universidade de Lisboa Instituto Superior Tecnico, Portugal
1998/03/01 - 2000/09/30 Associate Professor (University Teacher) Universidade Lusofona de Humanidades e Tecnologias, Portugal
1994/03/01 - 1998/02/28 Assistant Professor (University Teacher) Universidade Lusofona de Humanidades e Tecnologias, Portugal
Projects

Grant

Designation Funders
2022/01 - 2024/12 Mecanismos de fixação e durabilidade do tratamento de solos por biocimentação. Aplicação a um talude real para evitar ravinamento.
Provided by PTCRIS: PTDC/ECI-EGC/1086/2021
 Fundação para a Ciência e a Tecnologia
2022/01 - 2024/12 Plataforma de produção de células NK modificadas com CAR e suas vesículas extracelulares para imunoterapia
Provided by PTCRIS: PTDC/EQU-EQU/3708/2021
 Fundação para a Ciência e a Tecnologia
2018/10 - 2022/03 Utilização de biofilmes de Escherichia coli para a produção de proteínas recombinantes e outros compostos de elevado valor acrescentado
Provided by PTCRIS: PTDC/BII-BIO/29589/2017
 Fundação para a Ciência e a Tecnologia
2018/09 - 2021/12 Funcionalização de Materiais à Base de Celulose com Biomoléculas e Nanoestruturas Utilizando Estratégias de Reconhecimento Biomolecular
Provided by PTCRIS: PTDC/CTM-CTM/30790/2017
 Fundação para a Ciência e a Tecnologia
2014/03 - 2015/05 Vacinas de DNA contendo a subunidade hemaglutinina do vírus da gripe aviária e sequências de direccionamento para uso num contexto DIVA
Provided by PTCRIS: EXPL/CVT-EPI/0360/2013
133382
133382
Provided by PTCRIS: 133382
EXPL/CVT-EPI/0360/2013
 Fundação para a Ciência e a Tecnologia
2010/05 - 2013/11 SYNBIOBACTHER - Construção de bactérias "terapêuticas" usando uma abordagem de biologia sintética
Provided by PTCRIS: PTDC/EBB-BIO/102863/2008
 Fundação para a Ciência e a Tecnologia
2007/10 - 2010/12 Caracterização Biológica e Molecular de Vírus da Gripe Aviária e Desenvolvimento de Testes DIVA
Provided by PTCRIS: PTDC/CVT/67189/2006
 Fundação para a Ciência e a Tecnologia
2007/01 - 2009/03 Overcoming the nucleolytic barrier during trafficking of plasmid DNA vectors
Provided by PTCRIS: 82365
82365
 Fundação para a Ciência e a Tecnologia, I.P.
2005/07 - 2007/12 Bombas de efluxo em Mycobacterium tuberculosis: caracterizacão molecular dos mecanismos de efluxo e uso de inibidores de efluxo como novos compostos anti-bacilares
Provided by PTCRIS: POCI/SAU-MMO/59370/2004
 Fundação para a Ciência e a Tecnologia

Contract

Designation Funders
2018/05/01 - Current Phage4BacID, Bacteriófagos no Diagnóstico e Prevenção de Infecções Multiresistentes em Portugal e no Mundo
111
Researcher
Universidade de Lisboa Instituto de Bioengenharia e Biociencias, Portugal
Ongoing
2021/09/01 - 2025/08/31 Urinary bladder bioprinting for fully autologous transplantation
964883
10.3030/964883
Researcher
European Commission
Ongoing
2020/09/01 - 2025/08/31 AMELIE - Anchored Muscle cELls for IncontinencE
874807
10.3030/874807
Researcher
European Commission
Ongoing
2022/01/01 - 2024/12/31 Fixation mechanisms and durability of biocementation treatment of soils. Application to a real slope to prevent ravine development.
PTDC/ECI-EGC/1086/2021
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto de Investigação e Inovação em Engenharia Civil para a Sustentabilidade, Portugal

INESC Microsistemas e Nanotecnologias, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2022/01/01 - 2024/12/31 Quality by design-centered platform for the manufacturing of CAR-engineered NK cells and extracellular vesicles for immunotherapy
PTDC/EQU-EQU/3708/2021
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Instituto Politécnico de Lisboa Instituto Superior de Engenharia de Lisboa, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2020/10/01 - 2023/09/30 CAPTURA- Uso de partículas funcionalizadas para enriquecimento e detecção eficiente de SARS-CoV-2 em amostras clínicas e ambientais
72536
Universidade de Lisboa Instituto Superior Técnico, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2020/10/01 - 2023/06/30 Functionalized nanoparticles for enrichment and efficient detection of SARS-CoV-2 in<br>clinical and environmental samples
SAICTCOVID/72536/2020
Universidade de Lisboa Instituto Superior Técnico, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2018/10/01 - 2022/05/31 The use of Escherichia coli biofilms for the production of recombinant proteins and other added-value compounds
PTDC/BII-BIO/29589/2017
Universidade do Porto, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2018/10/01 - 2022/04/30 Lactic acid bacteria as cell factories: a Synthetic Biology approach for plasmid DNA and recombinant protein production
PTDC/BTM-SAL/28624/2017
Provided by PTCRIS: PTDC/BTM-SAL/28624/2017
Principal investigator
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2018/09/01 - 2021/12/31 Biorecognition as a Tool for the Functionalization of Cellulose-based Materials with Biomolecules and Nanostructures
PTDC/CTM-CTM/30790/2017
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2020/07/01 - 2020/12/31 LFA4Covid: Development of a Lateral Flow Assay working prototype for detection of antibodies to SARS-CoV-2
Research COVID 19-576
Researcher
Universidade de Lisboa Instituto de Bioengenharia e Biociencias, Portugal
Concluded
2013 - 2016 Gaining Productivity, Cost Efficiency and Sustainability in the Downstream Processing of Bio-Products by novel Integration and Intensification strategies
FP7-KBBE-2012-6
Researcher
Directorate-General for Research and Innovation
2014/03 - 2015/02 Avian influenza hemagglutinin subunit DNA vaccines with targeting sequences in frame with DIVA strategy
133382
Provided by PTCRIS: 133382
EXPL/CVT-EPI/0360/2013
Provided by PTCRIS: EXPL/CVT-EPI/0360/2013
Researcher
Universidade de Lisboa Instituto Superior Tecnico, Portugal

Instituto Nacional de Investigação Agrária e Veterinária, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia, I.P.

Fundação para a Ciência e a Tecnologia
Concluded
2011/01/01 - 2013/12/31 Improvement of the immunogenicity of DNA vaccines
PTDC/EBB-EBI/113650/2009
Provided by PTCRIS: PTDC/EBB-EBI/113650/2009
Principal investigator
Universidade de Lisboa Instituto Superior Técnico, Portugal

Instituto de Biotecnologia e Bioengenharia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2010/05 - 2013/11 SYNBIOBACTHER - Synthetic biology approaches to engineer "therapeutic" bacteria
102863
Provided by PTCRIS: 102863
PTDC/EBB-BIO/102863/2008
Researcher
Instituto de Biotecnologia e Bioengenharia, Portugal

Universidade do Minho, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia, I.P.

Fundação para a Ciência e a Tecnologia
Concluded
2011/01/01 - 2012/12/31 Projecto Estratégico - LA 18 - 2011-2012
PEst-OE/SAU/LA0018/2011
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2010/01 - 2012/12 A Lab-on-a-chip-based 2D fluorescence detection system for organism/cell condition analysis (Micro-2D-FS)
104387
Provided by PTCRIS: 104387
PTDC/CTM/104387/2008
Provided by PTCRIS: PTDC/CTM/104387/2008
Researcher
INESC Microsistemas e Nanotecnologias, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia, I.P.

Fundação para a Ciência e a Tecnologia
Concluded
2007/01 - 2009/06 Efflux-pumps in drug resistance of Mycobacterium tuberculosis: Molecular characterisation of the efflux mechanism and use of efflux inhibitors as new anti-tubercular compounds
Provided by PTCRIS: 82329
82329
Provided by PTCRIS: 59370
59370
Provided by PTCRIS: 61090
61090
 Fundação para a Ciência e a Tecnologia, I.P.
2007/01 - 2008/09 Electronically-addressed BioChips (eBioArray)
82459
Provided by PTCRIS: 82459
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
2005/02/01 - 2008/01/31 Development of experimental DNA vaccines against Maedi-Visna virus
OCTI/CVT/47260/2002
Researcher
Universidade de Lisboa Instituto Superior Técnico, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2004 - 2008 Advanced Interactive Materials by Design-AIMS
NMP3-CT2004-500160
Researcher
Concluded
2005/07/01 - 2007/12/31 Development of DNA vaccine prototypes for veterinary trypanosomiasis
POCI/CVT/61090/2004
Provided by PTCRIS: POCI/CVT/61090/2004
Provided by PTCRIS: 82211
82211
POCTI(PPCDT)/CVT/61090/2004
Principal investigator
Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal

Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2005/07/01 - 2007/12/31 Efflux-pumps in drug resistance of Mycobacterium tuberculosis: Molecular characterisation of the efflux mechanism and use of efflux inhibitors as new anti-tubercular compounds
POCI/SAU-MMO/59370/2004
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal

Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2005/07/01 - 2007/12/31 Overcoming the nucleolytic barrier during trafficking of plasmid DNA vectors
POCI/BIO/55799/2004
Universidade de Lisboa Instituto Superior Técnico, Portugal

Fundação Calouste Gulbenkian, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2005/07 - 2007/10 Electronically-addressed BioChips (eBioArray)
Provided by PTCRIS: 60487
60487
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
2001/01/01 - 2003/12/31 Screening for hot spots in plasmids for gene therapy and DNA vaccination
POCTI/BIO/32875/2000
Principal investigator
Universidade de Lisboa Instituto Superior Técnico, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2001/01/01 - 2003/12/31 Development of microelectronically addressed DNA chips as generic platforms for genomic analysis
info:eu-repo/grantAgreement/FCT/POCI/34022/PT
Researcher
Universidade do Algarve Faculdade de Ciências e Tecnologia
Concluded
2000/09/01 - 2003/08/31 DYNAMICS AND CONFORMATION OF BIOMOLECULES ADSORBED ONTO SMART LATEX PARTICLES
info:eu-repo/grantAgreement/FCT/POCI/33866/PT
Researcher
Universidade do Algarve Faculdade de Ciências e Tecnologia
Concluded
1997 - 2000 Construção de um sistema geral de expressão genética bacteriano, para a produção e purificação de proteínas humanas recombinadas de alto valor comercial e terapêutico
FCT/ADI PRAXIS XXI Medida 3.1B
Researcher
Concluded
Outputs

Publications

Book chapter
  1. Duarte, S.O.D.; Monteiro, G.A.. "A Real-Time Quantitative PCR Protocol for the Quantification of Plasmid Copy Number in Lactococcus lactis". 151-157. 2023.
    10.1007/978-1-0716-3358-8_12
  2. Alves, C.P.A.; Prazeres, D.M.F.; Monteiro, G.A.. "Real-Time PCR Method for Assessment of ParA-Mediated Recombination Efficiency in Minicircle Production". 117-131. 2023.
    10.1007/978-1-0716-3358-8_10
  3. "Gene delivery as a tool to improve therapeutic features of mesenchymal stromal cells: methods and applications". In Gene delivery: methods and applications. United States: Nova Science Publishers, Inc., New York, 2019.
    Published
  4. Cardoso, Rafaela; Pedreira, Rita; Duarte, Sofia; Monteiro, Gabriel; Borges, Hugo; Flores-Colen, Inês. "Biocementation as Rehabilitation Technique of Porous Materials". In New Approaches to Building Pathology and Durability, 99-120. Springer Singapore, 2016.
    10.1007/978-981-10-0648-7_5
  5. Pereira, P.M.M.; Monteiro, G.A.; Prazeres, D.M.F.. "General aspects of biomimetic materials". 57-79. 2015.
  6. "Plasmid biopharmaceuticals". In Plasmids: Biology and impact in biotechnology and discovery, 669-688. United States: ASM press, Washington DC, 2015.
    Published • 10.1128/microbiolspec. PLAS-0022-2014
  7. Matos, Joana; Monteiro, Gabriel; Goncavles, Clara. "Silica and ORMOSIL nanoparticles for gene delivery". In Nano based drug delivery, 239-265. IAPC Publishing, 2015.
    10.5599/obp.8.9
  8. "DNA instability in bacterial genomes: causes and consequences". In Genome Analysis: Current Procedures and Applications, 263-285. United Kingdom: Caister Academic Press, 2014.
    Published
  9. Monteiro, G.M.; Silva, M.S.; Henriques, A.M.; Carvalho, J.A.; Atouguia, J.; Fevereiro, M.; Prazeres, D.M.F.. "Advances in DNA vaccination: Design, immunology and manufacturing". 131-157. 2013.
  10. Oliveira, P.H.; Prazeres, D.M.F.; Monteiro, G.A.. "Structural and segregational instability in plasmid biology". 79-99. 2012.
  11. "DNA Vaccines: Construction, production and in vivo testing". In A Portrait of State-of-the-art Research at the Technical University of Lisbon, 219-232. Springer, The Netherlands, 2007.
    Published
  12. Mergulhão, Filipe J.; Monteiro, Gabriel A.; Mergulhao, Filipe J.. "Periplasmic Targeting of Recombinant Proteins in Escherichia coli". In Protein Targeting Protocols, 47-61. Humana Press, 2007.
    10.1007/978-1-59745-466-7_4
  13. "Essential guides for isolation/purification of nucleic acids". In Encyclopedia of Separation Science, 4560-4568. United Kingdom: Academic Press Ldt., London, 2000.
    Published
Conference paper
  1. Centeno Dias, F.; Borges, I.; Duarte, S.O.D.; Monteiro, G.A.; Cardoso, R.. "Comparison of experimental techniques for biocementation of sands considering homogeneous volume distribution of precipitated calcium carbonate". 2020.
    10.1051/e3sconf/202019505004
  2. Albuquerque, D.C.; Cardoso, R.; Monteiro, G.A.; Duarte, S.O.; Martins, V.C.; Cardoso, S.. "Towards a portable magnetoresistive biochip for urease-based biocementation monitoring". 2019.
    10.1109/ENBENG.2019.8692505
  3. Ulpiano, C.; Alves, C.A.P.; Silva-Santos, A.R.; Serra, J.; Da Silva, C.L.; Monteiro, G.A.. "RNAi as a tool to inhibit the angiogenic potential of human Mesenchymal Stem/Stromal Cells in malignancy". 2019.
    10.1109/ENBENG.2019.8692558
  4. Felício, F.; Silverio, V.; Duarte, S.; Galvão, A.; Monteiro, G.; Cardoso, S.; Cardoso, R.. "Preliminary tests on a microfluidic device to study pore clogging during biocementation". 2019.
    10.1051/e3sconf/20199211018
  5. Cardoso, R.; Borges, H.; Flores-Colen, I.; Monteiro, G.. "Experimental study on using biodeposition in mortar surfaces". 2016.
  6. Prazeres, D.M.F.; Martins, S.A.M.; Trabuco, J.R.C.; Monteiro, G.A.; Juskowiak, A.; Conde, J.P.; Chu, V.. "Towards a high-throughput drug discovery platform for the screening of GPCR targets in cells". 2011.
    10.1109/ENBENG.2011.6026042
Conference poster
  1. Gomes, Luciana C.; Monteiro, Gabriel António Amaro; Filipe J. M. Mergulhão. "Curcumin production by engineered Escherichia coli biofilms". Paper presented in SurfSAFE Conference - Biofilm detection and control in the food industry, 2023.
  2. Carvalho, Fábio; Monteiro, Gabriel António Amaro; Gomes, Luciana C.; Filipe J. M. Mergulhão. "Expression of recombinant Green Fluorescent Protein (GFP) using Escherichia coli biofilms: effects of culture media and surface properties". Paper presented in Biofilms 10, 2022.
  3. Duarte, Sofia O. D. ; Bruno Costa; 031C-B4B2-0B2E; Moreira, Leonilde M; Prazeres, Duarte Miguel F.; Pinheiro, Pedro F.; Justino, Gonçalo C.; Monteiro, Gabriel António Amaro. "Characterization of a novel ferulic acid pathway in Lactococcus lactis". Paper presented in Microbiotec21, 2021.
  4. 031C-B4B2-0B2E; 3510-7BD2-BCD1; Antunes, Diogo; Duarte, Sofia O. D. ; Moreira, Leonilde M; Monteiro, Gabriel António Amaro. "Genome Sequence and Annotation of Lactococcus lactis Strain LMG 19460 and Novel Strains IST-LL01 and IST-LL02". Paper presented in Microbiotec21, 2021.
  5. 031C-B4B2-0B2E; Diogo de Lucena; Bruno Costa; Duarte, Sofia O. D. ; Pinheiro, Pedro F.; Justino, Gonçalo C.; Moreira, Leonilde M; Monteiro, Gabriel António Amaro. "Reconstruction of the Metabolic Network of Lactococcus lactis strains IL1403 and LMG 19460". Paper presented in Microbiotec21, 2021.
  6. Silva, Bruno; Duarte, Sofia O. D. ; Moreira, Leonilde M; Monteiro, Gabriel António Amaro. "Screening of Lactic Acid Bacteria strains for Electrotransformation Efficiency". Paper presented in Microbiotec21, 2021.
  7. Azevedo, Ana; Gomes, Luciana Calheiros; Teixeira-Santos, Rita; Monteiro, Gabriel António Amaro; Mergulhão, Filipe. Corresponding author: Mergulhão, Filipe. "Expression of Green Fluorescent Protein (GFP) in Escherichia coli biofilms: impact of surface and nutrient conditions". Paper presented in IBBS'18 - International Biodeterioration & Biodegradation Symposium, 2021.
  8. 3510-7BD2-BCD1; Duarte, Sofia O. D. ; Prazeres, Duarte M. F.; Monteiro, Gabriel António Amaro. "Deletion of the nuclease gene nth leads to a decrease in plasmid DNA yields in Lactococcus lactis subsp. lactis LMG19460". Paper presented in 13th International Symposium on Lactic Acid Bacteria, 2021.
  9. Duarte, Sofia O. D. ; 031C-B4B2-0B2E; Moreira, Leonilde M; Prazeres, Duarte Miguel F.; Pinheiro, Pedro F.; Justino, Gonçalo C.; Monteiro, Gabriel António Amaro. "Identification of the ferulic acid metabolism in Lactococcus lactis". Paper presented in 13th International Symposium on Lactic Acid Bacteria, 2021.
  10. 031C-B4B2-0B2E; Diogo de Lucena; Duarte, Sofia O. D. ; Pinheiro, Pedro F.; Moreira, Leonilde M; Monteiro, Gabriel António Amaro. "Reconstruction of the Metabolic Network of Lactococcus lactis LMG 19460". Paper presented in 13th International Symposium on Lactic Acid Bacteria, 2021.
  11. Silva, Bruno; Duarte, Sofia O. D. ; Monteiro, Gabriel António Amaro. "A high copy number plasmid with a high level of heterologous protein expression for Lactic Acid Bacteria". Paper presented in 13th International Symposium on Lactic Acid Bacteria, 2021.
  12. Gomes, L. C. ; Monteiro, Gabriel António Amaro; Filipe J. M. Mergulhão. "Heterologous Protein Expression in E. coli Biofilms: Gene Dosage and Induction Effect". Paper presented in Biointerfaces International 2021 Zurich Conference Online Conference, 2021.
  13. Azevedo, Ana; Gomes, Luciana Calheiros; Teixeira-Santos, Rita; Monteiro, Gabriel António Amaro; Mergulhão, Filipe. Corresponding author: Mergulhão, Filipe. "Surface and nutrient media: impact on E. coli biofilms expressing Green Fluorescent Protein (GFP)". Paper presented in Biointerfaces International Zurich 2021, 2021.
  14. Duarte, Sofia O. D. ; Monteiro, Gabriel António Amaro. "Redesign of Lactococcus lactis genome for increasing pDNA and recombinant protein production using a CRISPR/Cas9 strategy". Paper presented in 2021 Synthetic Biology: Engineering, Evolution & Design (SEED), 2021.
  15. Azevedo, Ana; Gomes, Luciana Calheiros; Teixeira-Santos, Rita; Monteiro, Gabriel António Amaro; Mergulhão, Filipe. Corresponding author: Mergulhão, Filipe. "Optimization of enhanced Green Fluorescent Protein (eGFP) production in Escherichia coli biofilms". Paper presented in DCE 2021 ¿ Doctoral Congress in Engineering 2021, 2021.
  16. 3510-7BD2-BCD1; Duarte, Sofia O. D. ; Prazeres, Duarte M. F.; Monteiro, Gabriel António Amaro. "Application of a CRISPR/Cas9-based genome editing system for improved biopharmaceutical grade plasmid DNA and recombinant protein production in Lactococcus lactis". Paper presented in 5th Applied Synthetic Biology in Europe, 2020.
  17. Santos, José; Monteiro, Gabriel António Amaro; Prazeres, Miguel; Duarte, Sofia Oliveira Dias. "Adaptation of a one-plasmid genome editing system for increased production of biopharmaceutical grade recombinant molecules in Lactococcus lactis". Paper presented in Microbiotec19 - Congress of Microbiology and Biotechnology 2019, 2019.
Journal article
  1. Cristiana Ulpiano; Cláudia L. da Silva; Gabriel A. Monteiro. "Bioengineered Mesenchymal-Stromal-Cell-Derived Extracellular Vesicles as an Improved Drug Delivery System: Methods and Applications". Biomedicines (2023): https://doi.org/10.3390/biomedicines11041231.
    10.3390/biomedicines11041231
  2. Cardoso, R.; Borges, I.; Vieira, J.; Duarte, S.O.D.; Monteiro, G.A.. "Interactions between clay minerals, bacteria growth and urease activity on biocementation of soils". Applied Clay Science 240 (2023): http://www.scopus.com/inward/record.url?eid=2-s2.0-85159051928&partnerID=MN8TOARS.
    10.1016/j.clay.2023.106972
  3. Alexandra Soares; Luciana C. Gomes; Gabriel A. Monteiro; Filipe J. Mergulhão. "Hydrodynamic Effects on Biofilm Development and Recombinant Protein Expression". Microorganisms 10 5 (2022): 931-931. https://doi.org/10.3390/microorganisms10050931.
    10.3390/microorganisms10050931
  4. Gabriel A. Monteiro; Sofia O. D. Duarte. "The Effect of Recombinant Protein Production in Lactococcus lactis Transcriptome and Proteome". Microorganisms 10 2 (2022): 267-267. https://doi.org/10.3390/microorganisms10020267.
    10.3390/microorganisms10020267
  5. Cristiana Ulpiano; Cláudia L. da Silva; Gabriel A. Monteiro. "Mesenchymal Stromal Cells (MSCs): A Promising Tool for Cell-Based Angiogenic Therapy". Current Gene Therapy 21 (2021): https://doi.org/10.2174/1566523221666210917114353.
    10.2174/1566523221666210917114353
  6. Cláudia P.A. Alves; Duarte Miguel F. Prazeres; Gabriel A. Monteiro. "Recombination efficiency measurement by real-time PCR: A strategy to evaluate ParA-mediated minicircle production". Analytical Biochemistry 628 (2021): 114285-114285. https://doi.org/10.1016/j.ab.2021.114285.
    10.1016/j.ab.2021.114285
  7. Joana Serra; Cláudia P. A. Alves; Joaquim M. S. Cabral; Gabriel A. Monteiro; Cláudia L. Silva; Duarte Miguel F. Prazeres. "Minicircle-based expression of vascular endothelial growth factor in mesenchymal stromal cells from diverse human tissues". The Journal of Gene Medicine (2021): https://doi.org/10.1002/jgm.3342.
    10.1002/jgm.3342
  8. Monteiro, Gabriel António Amaro. "The influence of stone joints width and roughness on the efficiency of biocementation sealing". Construction and Building Materials (2021): https://www.sciencedirect.com/science/article/pii/S0950061821005031.
    10.1016/j.conbuildmat.2021.122743
  9. Monteiro, Gabriel António Amaro. "Minicircle Biopharmaceuticals–An Overview of Purification Strategies". (2021): https://www.frontiersin.org/articles/10.3389/fceng.2020.612594/full.
    10.3389/fceng.2020.612594
  10. Duarte, S.O.D.; Monteiro, G.A.. "Plasmid replicons for the production of pharmaceutical-grade pDNA, proteins and antigens by lactococcus lactis cell factories". International Journal of Molecular Sciences 22 3 (2021): 1-30. http://www.scopus.com/inward/record.url?eid=2-s2.0-85100040210&partnerID=MN8TOARS.
    10.3390/ijms22031379
  11. Gomes, L.; Monteiro, G.; Mergulhão, F.. "The impact of IPTG induction on plasmid stability and heterologous protein expression by escherichia coli biofilms". International Journal of Molecular Sciences 21 2 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85078252486&partnerID=MN8TOARS.
    10.3390/ijms21020576
  12. Cardoso, R.; Pedreira, R.; Duarte, S.O.D.; Monteiro, G.A.. "About calcium carbonate precipitation on sand biocementation". Engineering Geology 271 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85082662578&partnerID=MN8TOARS.
    10.1016/j.enggeo.2020.105612
  13. Silva, M.; Monteiro, G.A.; Fialho, A.M.; Bernardes, N.; da Silva, C.L.. "Conditioned Medium From Azurin-Expressing Human Mesenchymal Stromal Cells Demonstrates Antitumor Activity Against Breast and Lung Cancer Cell Lines". Frontiers in Cell and Developmental Biology 8 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85088539374&partnerID=MN8TOARS.
    10.3389/fcell.2020.00471
  14. Sofia O. D. Duarte; Maria C. Martins; Sílvia M. Andrade; Duarte M. F. Prazeres; Gabriel A. Monteiro. "Plasmid Copy Number of pTRKH3 in Lactococcus lactis is Increased by Modification of the repDE Ribosome-Binding Site". Biotechnology Journal 14 8 (2019): 1800587-1800587. https://doi.org/10.1002/biot.201800587.
    10.1002/biot.201800587
  15. Sofia O. D. Duarte; Maria C. Martins; Sílvia M. Andrade; Duarte M. F. Prazeres; Gabriel A. Monteiro. "Cover Picture: Biotechnology Journal 8/2019". Biotechnology Journal (2019): https://doi.org/10.1002/biot.201970081.
    10.1002/biot.201970081
  16. Silva-Santos, A.R.; Alves, C.P.A.; Monteiro, G.; Azevedo, A.M.; Prazeres, D.M.F.. "Multimodal chromatography of supercoiled minicircles: A closer look into DNA-ligand interactions". Separation and Purification Technology 212 (2019): 161-170. http://www.scopus.com/inward/record.url?eid=2-s2.0-85056601221&partnerID=MN8TOARS.
    10.1016/j.seppur.2018.11.015
  17. Cardoso, Rafaela; Pires, Ines; Duarte, Sofia O. D.; Monteiro, Gabriel A.. "Effects of clay's chemical interactions on biocementation". Applied Clay Science 156 (2018): 96-103. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000430620800012&KeyUID=WOS:000430620800012.
    10.1016/j.clay.2018.01.035
  18. Alves, Claudia P. A.; Simcikova, Michaela; Brito, Liliana; Monteiro, Gabriel A.; Prazeres, Duarte Miguel F.. "Production and Purification of Supercoiled Minicircles by a Combination of In Vitro Endonuclease Nicking and Hydrophobic Interaction Chromatography". Human Gene Therapy Methods 29 4 (2018): 157-168. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000442383500001&KeyUID=WOS:000442383500001.
    10.1089/hgtb.2018.046
  19. Pandey, Rajat; Kumar, Nitin; Monteiro, Gabriel A.; Veeranki, Venkata Dasu; Prazeres, D. M. F.. "Re-engineering of an Escherichia coli K-12 strain for the efficient production of recombinant human Interferon Gamma". Enzyme and Microbial Technology 117 (2018): 23-31. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000440882000004&KeyUID=WOS:000440882000004.
    10.1016/j.enzmictec.2018.06.001
  20. Serra, Joana; Alves, Claudia P. A.; Brito, Liliana; Monteiro, Gabriel A.; Cabral, Joaquim M. S.; Prazeres, Duarte Miguel F.; da Silva, Claudia L.. "Engineering of Human Mesenchymal Stem/Stromal Cells with Vascular Endothelial Growth Factor-Encoding Minicircles for Angiogenic Ex Vivo Gene Therapy". Human Gene Therapy (2018): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000449926200001&KeyUID=WOS:000449926200001.
    10.1089/hum.2018.154
  21. Rauta, Pradipta R.; Nayak, Bismita; Monteiro, Gabriel A.; Mateus, Marilia. "Design and characterization of plasmids encoding antigenic peptides of Aha1 from Aeromonas hydrophila as prospective fish vaccines". Journal of Biotechnology 241 (2017): 116-126. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000392677900017&KeyUID=WOS:000392677900017.
    10.1016/j.jbiotec.2016.11.019
  22. Silva, Ines N; Duarte, Sofia; Moreira, Leonilde M; Monteiro, Gabriel A. "Draft Genome Sequence of the Plasmid-Free Lactococcus lactis subsp. lactis Strain LMG 19460.". Genome announcements 5 16 (2017): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:28428305&KeyUID=MEDLINE:28428305.
    10.1128/genomeA.00210-17
  23. Dias, T. M.; Fernandes, E.; Cardoso, S.; Monteiro, G.; Freitas, P. P.. "One-step trapping of droplets and surface functionalization of sensors using gold-patterned structures for multiplexing in biochips (vol 7, pg 43273, 2017)". Rsc Advances 7 73 (2017): 46124-46124. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000412211300029&KeyUID=WOS:000412211300029.
    10.1039/c7ra90095c
  24. Matos, J. C.; Soares, A. R.; Domingues, I.; Monteiro, G. A.; Goncalves, M. C.. "ORMOPLEXEs for gene therapy: In vitro and in vivo assays". Materials Science & Engineering C-Materials For Biological Applications 63 (2016): 546-553. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000374916800061&KeyUID=WOS:000374916800061.
    10.1016/j.msec.2016.03.006
  25. Alves, Claudia P. A.; Duarte, Sofia O. D.; Monteiro, Gabriel A.; Prazeres, Duarte Miguel F.. "Use of an E. coli pgi Knockout Strain as a Plasmid Producer". Biopharm International 29 2 (2016): 38-42. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000370766200008&KeyUID=WOS:000370766200008.
  26. Alves, Claudia P. A.; Simcikova, Michaela; Brito, Liliana; Monteiro, Gabriel A.; Prazeres, Duarte Miguel F.. "Development of a nicking endonuclease-assisted method for the purification of minicircles". Journal of Chromatography a 1443 (2016): 136-144. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000374362600017&KeyUID=WOS:000374362600017.
    10.1016/j.chroma.2016.03.035
  27. Henriques, Ana M; Fevereiro, Miguel; Monteiro, Gabriel A. "DNA Vaccines Against Maedi-Visna Virus.". Methods in molecular biology (Clifton, N.J.) 1404 (2016): 59-76. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:27076290&KeyUID=MEDLINE:27076290.
    10.1007/978-1-4939-3389-1_4
  28. Dias, T. M.; Cardoso, F. A.; Martins, S. A. M.; Martins, V. C.; Cardoso, S.; Gaspar, J. F.; Monteiro, G.; Freitas, P. P.. "Implementing a strategy for on-chip detection of cell-free DNA fragments using GMR sensors: A translational application in cancer diagnostics using ALU elements". Analytical Methods 8 1 (2016): 119-128. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000366905700016&KeyUID=WOS:000366905700016.
    10.1039/c5ay01587a
  29. Simcikova, Michaela; Alves, Cludia P. A.; Brito, Liliana; Prather, Kristala L. J.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Improvement of DNA minicircle production by optimization of the secondary structure of the 5 '-UTR of ParA resolvase". Applied Microbiology and Biotechnology 100 15 (2016): 6725-6737. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000379317300018&KeyUID=WOS:000379317300018.
    10.1007/s00253-016-7565-x
  30. Jonathan De la, Vega; Gabriel, A. Monteiro and Duarte Miguel F. Prazeres. "Use of DNA Stabilizers to Extend Plasmid Biological Activity". Current Bionanotechnology (Discontinued) 1 2 (2015): 102-109. http://www.eurekaselect.com/node/137823/article.
    http://dx.doi.org/10.2174/2213529402666151214222447
  31. Monteiro, Gabriel António Amaro. "Towards effective non-viral gene delivery vector". Biotechnology and Genetic Engineering Reviews, Vol 31, Issue 1-2 31 (2015): 82-107. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000403312800005&KeyUID=WOS:000403312800005.
    10.1080/02648725.2016.1178011
  32. Lopes, Marta B.; Goncalves, Geisa A. L.; Felicio-Silva, Daniel; Prather, Kristala L. J.; Monteiro, Gabriel A.; Prazeres, Duarte M. F.; Calado, Cecilia R. C.. "In situ NIR spectroscopy monitoring of plasmid production processes: effect of producing strain, medium composition and the cultivation strategy". Journal of Chemical Technology and Biotechnology 90 2 (2015): 255-261. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000347778100006&KeyUID=WOS:000347778100006.
    10.1002/jctb.4431
  33. Goncalves, Geisa A. L.; Prather, Kristala L. J.; Monteiro, Gabriel A.; Carnes, Aaron E.; Prazeres, Duarte M. F.. "Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: Fermentation strategies and impact on downstream processing". Journal of Biotechnology 186 (2014): 119-127. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000343836000014&KeyUID=WOS:000343836000014.
    10.1016/j.jbiotec.2014.06.008
  34. Magalhaes, Salome; Duarte, Sofia; Monteiro, Gabriel A.; Fernandes, Fabio. "Quantitative Evaluation of DNA Dissociation from Liposome Carriers and DNA Escape from Endosomes During Lipid-Mediated Gene Delivery". Human Gene Therapy Methods 25 5 (2014): 303-313. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000345826900004&KeyUID=WOS:000345826900004.
    10.1089/hgtb.2014.080
  35. Goncalves, Geisa A. L.; Oliveira, Pedro H.; Gomes, Ana G.; Prather, Kristala L. J.; Lewis, Leslie A.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters". Applied Microbiology and Biotechnology 98 15 (2014): 6609-6619. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000339880300007&KeyUID=WOS:000339880300007.
    10.1007/s00253-014-5695-6
  36. Martins, S. A. M.; Moulas, G.; Trabuco, J. R. C.; Monteiro, G. A.; Chu, V.; Conde, J. P.; Prazeres, D. M. F.. "Monitoring intracellular calcium in response to GPCR activation using thin-film silicon photodiodes with integrated fluorescence filters". Biosensors & Bioelectronics 52 (2014): 232-238. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000328095400035&KeyUID=WOS:000328095400035.
    10.1016/j.bios.2013.08.037
  37. Simcikova, Michaela; Prather, Kristala L. J.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "On the dual effect of glucose during production of pBAD/AraC-based minicircles". Vaccine 32 24 (2014): 2843-2846. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000336704900014&KeyUID=WOS:000336704900014.
    10.1016/j.vaccine.2014.02.035
  38. Prazeres, Duarte Miguel F; Monteiro, Gabriel A. "Plasmid Biopharmaceuticals.". Microbiology spectrum 2 6 (2014): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:26104457&KeyUID=MEDLINE:26104457.
    10.1128/microbiolspec.PLAS-0022-2014
  39. Matos Pereira, Pedro Miguel; Moita, Ana Sofia; Monteiro, Gabriel Amaro; Franca Prazeres, Duarte Miguel. "Characterization of the Topography and Wettability of English Weed Leaves and Biomimetic Replicas". Journal of Bionic Engineering 11 3 (2014): 346-359. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000339601200002&KeyUID=WOS:000339601200002.
    10.1016/S1672-6529(14)60048-2
  40. Goncalves, Geisa A. L.; Prather, Kristala L. J.; Monteiro, Gabriel A.; Prazeres, Duarte M. F.. "Engineering of Escherichia coli strains for plasmid biopharmaceutical production: Scale-up challenges". Vaccine 32 24 (2014): 2847-2850. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000336704900015&KeyUID=WOS:000336704900015.
    10.1016/j.vaccine.2014.02.023
  41. Freitas, Elisabete Borges; Henriques, Ana Margarida; Fevereiro, Miguel; Prazeres, Duarte Miguel; Monteiro, Gabriel Amaro. "Enhancement of DNA vaccine efficacy by intracellular targeting strategies.". Methods in molecular biology (Clifton, N.J.) 1143 (2014): 33-59. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:24715281&KeyUID=MEDLINE:24715281.
    10.1007/978-1-4939-0410-5_4
  42. Goncalves, Geisa A. L.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.; Prather, Kristala L. J.. "De novo creation of MG1655-derived E-coli strains specifically designed for plasmid DNA production". Applied Microbiology and Biotechnology 97 2 (2013): 611-620. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000313651700012&KeyUID=WOS:000313651700012.
    10.1007/s00253-012-4308-5
  43. De La Vega, Jonathan; Ter Braak, Bas; Azzoni, Adriano R.; Monteiro, Gabriel A.; Prazeres, Duarte Miguel F.. "Impact of Plasmid Quality on Lipoplex-Mediated Transfection". Journal of Pharmaceutical Sciences 102 11 (2013): 3932-3941. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000325550400009&KeyUID=WOS:000325550400009.
    10.1002/jps.23709
  44. Lewis, Leslie A.; Astatke, Mekbib; Umekubo, Peter T.; Alvi, Shaheen; Saby, Robert; Afrose, Jehan; Oliveira, Pedro H.; Monteiro, Gabriel A.; Prazeres, Duarte M. F.. "Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition". Mobile DNA 3 (2012): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000318291100001&KeyUID=WOS:000318291100001.
    10.1186/1759-8753-3-1
  45. Goncalves, Geisa A. L.; Bower, Diana M.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.; Prather, Kristala L. J.. "Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing". Biotechnology Journal 7 2 (2012): 251-261. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000302022900009&KeyUID=WOS:000302022900009.
    10.1002/biot.201100062
  46. Martins, Sofia A. M.; Trabuco, Joao R. C.; Monteiro, Gabriel A.; Chu, Virginia; Conde, Joao P.; Prazeres, D. Miguel F.. "Towards the miniaturization of GPCR-based live-cell screening assays". Trends in Biotechnology 30 11 (2012): 566-574. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000310939900003&KeyUID=WOS:000310939900003.
    10.1016/j.tibtech.2012.07.004
  47. Toledo, Marcelo A. S.; Janissen, Richard; Favaro, Marianna T. P.; Cotta, Monica A.; Monteiro, Gabriel A.; Prazeres, Duarte Miguel F.; Souza, Anete P.; Azzoni, Adriano R.. "Development of a recombinant fusion protein based on the dynein light chain LC8 for non-viral gene delivery". Journal of Controlled Release 159 2 (2012): 222-231. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000305001100009&KeyUID=WOS:000305001100009.
    10.1016/j.jconrel.2012.01.011
  48. Ribeiro, Sofia; Mairhofer, Juergen; Madeira, Catarina; Diogo, Maria Margarida; da Silva, Claudia Lobato; Monteiro, Gabriel; Grabherr, Reingard; Cabral, Joaquim M.. "Plasmid DNA Size Does Affect Nonviral Gene Delivery Efficiency in Stem Cells". Cellular Reprogramming 14 2 (2012): 130-137. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000302523600005&KeyUID=WOS:000302523600005.
    10.1089/cell.2011.0093
  49. Monteiro, Gabriel António Amaro; Martins, S.M.A.; Trabuco, J.R.G.; Monteiro, G.A.; Prazeres, D.M.. "GPCR screening and drug discovery: Challenges and latest trends". European Pharm. Rev. 17, 57-62 (2012): https://www.europeanpharmaceuticalreview.com/article/12421/gpcr-screening-and-drug-discovery-challenges-and-latest-trends/,.
  50. Goncalves, G. A. L.; Oliveira, P. H.; Lewis, L. A.; Prazeres, D. M. F.; Monteiro, G. A.. "Identification of IS2 transposition in pVAX1-based plasmid, a common vector for DNA vaccine development". New Biotechnology 29 (2012): S205-S205. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000209805600568&KeyUID=WOS:000209805600568.
    10.1016/j.nbt.2012.08.575
  51. Martins, S. A. M.; Moulas, G.; Trabuco, J. R. C.; Monteiro, G. A.; Chu, V.; Conde, J. P.; Prazeres, D. M. F.; Walczak, R; Dziuban, J. "Integrated on-chip photodetection of intracellular calcium in response to the activation of G-protein coupled receptors". 26th European Conference on Solid-State Transducers, Eurosensor 2012 47 (2012): 993-996. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000312953300245&KeyUID=WOS:000312953300245.
    10.1016/j.proeng.2012.09.314
  52. Oliveira, Pedro H.; Prather, Kristala L. J.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Mutation detection in plasmid-based biopharmaceuticals". Biotechnology Journal 6 4 (2011): 378-391. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000289214500003&KeyUID=WOS:000289214500003.
    10.1002/biot.201000372
  53. Cruz Lanca, Andreia Sofia; de Sousa, Karina Fires; Atouguia, Jorge; Prazeres, Duarte Miguel F.; Monteiro, Gabriel Amaro; Silva, Marcelo Sousa. "Trypanosoma brucei: Immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis". Experimental Parasitology 127 1 (2011): 18-24. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000286486800004&KeyUID=WOS:000286486800004.
    10.1016/j.exppara.2010.06.017
  54. Martins, S. A. M.; Prazeres, D. M. F.; Fonseca, L. P.; Monteiro, G. A.. "Quantitation of non-amplified genomic DNA by bead-based hybridization and template mediated extension coupled to alkaline phosphatase signal amplification". Biotechnology Letters 32 2 (2010): 229-234. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000273683000008&KeyUID=WOS:000273683000008.
    10.1007/s10529-009-0149-4
  55. Carvalho, Joana A.; Azzoni, Adriano R.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.; Carvalho, JA; Azzoni, AR; Prazeres, DMF; Monteiro, GA. "Comparative Analysis of Antigen-Targeting Sequences Used in DNA Vaccines". Molecular Biotechnology 44 3 (2010): 204-212. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000274303900004&KeyUID=WOS:000274303900004.
    10.1007/s12033-009-9229-x
  56. Carvalho, Joana A.; Rodgers, Jean; Atouguia, Jorge; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "DNA vaccines: a rational design against parasitic diseases". Expert Review of Vaccines 9 2 (2010): 175-191. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000274499300011&KeyUID=WOS:000274499300011.
    10.1586/erv.09.158
  57. Ribeiro, Sofia C.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Evidence for the in vivo expression of a distant downstream gene under the control of ColE1 replication origin". Applied Microbiology and Biotechnology 86 2 (2010): 671-679. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000274707600027&KeyUID=WOS:000274707600027.
    10.1007/s00253-009-2339-3
  58. Martins, S. A. M.; Prazeres, D. M. F.; Fonseca, L. P.; Monteiro, G. A.. "Optimization of DNA Hybridization on Aminopropyl-Controlled Pore-Glass Particles: Detection of Non-Labeled Targets by PicoGreen Staining". Analytical Letters 43 17 (2010): 2694-2704. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000284891000005&KeyUID=WOS:000284891000005.
    10.1080/00032711003731431
  59. Ribeiro, S. C.; Mendes, R.; Madeira, C.; Monteiro, G. A.; da Silva, C. L.; Cabral, J. M. S.. "A Quantitative Method to Evaluate Mesenchymal Stem Cell Lipofection Using Real-Time PCR". Biotechnology Progress 26 5 (2010): 1501-1504. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000283482100036&KeyUID=WOS:000283482100036.
    10.1002/btpr.451
  60. Oliveira, Pedro H.; Prather, Kristala Jones; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events". Applied Microbiology and Biotechnology 87 6 (2010): 2157-2167. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000280373100018&KeyUID=WOS:000280373100018.
    10.1007/s00253-010-2671-7
  61. Alexandra Soares; Luciana C. Gomes; Gabriel A. Monteiro; Filipe J. Mergulhão. "The Influence of Nutrient Medium Composition on Escherichia coli Biofilm Development and Heterologous Protein Expression". Applied Sciences (2010): https://doi.org/10.3390/app11188667.
    10.3390/app11188667
  62. Oliveira, P.H.; Prather, K.J.; Prazeres, D.M.F.; Monteiro, G.A.. "Erratum: Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events (Applied Microbiology and Biotechnology DOI: 10.1007/s00253-010-2671-7)". Applied Microbiology and Biotechnology 87 6 (2010): 2373-2373. http://www.scopus.com/inward/record.url?eid=2-s2.0-77955536867&partnerID=MN8TOARS.
    10.1007/s00253-010-2745-6
  63. Martins, S. A. M.; Prazeres, D. M. F.; Fonseca, L. P.; Monteiro, G. A.. "Application of central composite design for DNA hybridization onto magnetic microparticles". Analytical Biochemistry 391 1 (2009): 17-23. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000267063200003&KeyUID=WOS:000267063200003.
    10.1016/j.ab.2009.05.006
  64. Carvalho, Joana A.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Bringing DNA vaccines closer to commercial use". Idrugs 12 10 (2009): 642-647. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000270158300012&KeyUID=WOS:000270158300012.
  65. Oliveira, Pedro H.; Prather, Kristala Jones; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Structural instability of plasmid biopharmaceuticals: challenges and implications". Trends in Biotechnology 27 9 (2009): 503-511. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000269868000002&KeyUID=WOS:000269868000002.
    10.1016/j.tibtech.2009.06.004
  66. Martins, S. A. M.; Prazeres, D. M. F.; Fonseca, L. P.; Monteiro, G. A.. "The role of probe-probe interactions on the hybridization of double-stranded DNA targets onto DNA-modified magnetic microparticles". Analytical and Bioanalytical Chemistry 394 6 (2009): 1711-1716. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000267329400024&KeyUID=WOS:000267329400024.
    10.1007/s00216-009-2843-4
  67. Silva, Marcelo Sousa; Prazeres, Duarte Miguel F.; Lanca, Andreia; Atouguia, Jorge; Monteiro, Gabriel Amaro. "Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis". Parasitology Research 105 5 (2009): 1223-1229. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000269914800005&KeyUID=WOS:000269914800005.
    10.1007/s00436-009-1542-6
  68. Ribeiro, S. C.; Monteiro, G. A.; Prazeres, D. M. F.. "Evaluation of the Effect of Non-B DNA Structures on Plasmid Integrity Via Accelerated Stability Studies". Journal of Pharmaceutical Sciences 98 4 (2009): 1400-1408. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000264851400010&KeyUID=WOS:000264851400010.
    10.1002/jps.21503
  69. Paixao, Laura; Rodrigues, Liliana; Couto, Isabel; Martins, Marta; Fernandes, Pedro; de Carvalho, Carla C C R; Monteiro, Gabriel A; et al. "Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli.". Journal of biological engineering 3 (2009): 18-18. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:19835592&KeyUID=MEDLINE:19835592.
    10.1186/1754-1611-3-18
  70. Wu, Ming L.; Freitas, Sindelia S.; Monteiro, Gabriel A.; Prazeres, Duarte M. F.; Santos, Jose A. L.. "Stabilization of naked and condensed plasmid DNA against degradation induced by ultrasounds and high-shear vortices". Biotechnology and Applied Biochemistry 53 (2009): 237-246. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000268925700003&KeyUID=WOS:000268925700003.
    10.1042/ba20080215
  71. Oliveira, Pedro H.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Deletion formation mutations in plasmid expression vectors are unfavored by runaway amplification conditions and differentially selected under kanamycin stress". Journal of Biotechnology 143 4 (2009): 231-238. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000271042700001&KeyUID=WOS:000271042700001.
    10.1016/j.jbiotec.2009.08.002
  72. Henriques, A. M.; Madeira, C.; Fevereiro, M.; Prazeres, D. M. F.; Aires-Barros, M. R.; Monteiro, G. A.. "Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus". International Journal of Pharmaceutics 377 1-2 (2009): 92-98. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000268471300012&KeyUID=WOS:000268471300012.
    10.1016/j.ijpharm.2009.05.005
  73. Madeira, CP; Ribeiro, SC; dos Santos, F; Monteiro, GA; da Silva, L; Cabral, JMS. "COMPARISON OF POLYADENYLATION SIGNALS FOR USE IN TRANSIENT GENETIC MODIFICATION OF HUMAN MESENCHYMAL STEM CELLS". Experimental Hematology 37 9 (2009): S97-S98. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000269396400194&KeyUID=WOS:000269396400194.
  74. de Toledo, MAS; Monteiro, G; Prazeres, M; de Souza, A; Azzoni, A. "The use of recombinant dynein light chains to mediate enhanced plasmidial DNA transfection". Human Gene Therapy 20 11 (2009): 1441-1441. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000271441000267&KeyUID=WOS:000271441000267.
  75. Cruz Lanca, A. S.; Pires de Sousa, K.; Atouguia, J.; Amaro Monteiro, G.; Miguel Prazeres, D.; Sousa Silva, M.; Schmidt, RE. "Induction of humoral response following immunization with three different plasmid DNA vaccines against African trypanosomiasis". 2nd European Congress of Immunology (Eci) (2009): 459-464. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000292190700090&KeyUID=WOS:000292190700090.
  76. de Toledo, Marcelo Szymanski; Monteiro, Gabriel; Prazeres, Miguel; Souza, Anete; Azzoni, Adriano. "Development of recombinant proteins for non viral gene delivery taking advantage of microtubule motor proteins". Human Gene Therapy 20 11 (2009): 1438-1438. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000271441000259&KeyUID=WOS:000271441000259.
  77. Martins, SA; Prazeres, MD; Fonseca, LP; Monteiro, G. "DNA biosensors: towards a microparticle based-platform for genomic DNA detection". New Biotechnology 25 (2009): S19-S20. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000281760100045&KeyUID=WOS:000281760100045.
    10.1016/j.nbt.2009.06.049
  78. Cardoso, M. A. Tavares; Monteiro, G. A.; Cardoso, J. P.; Prazeres, T. J. V.; Figueiredo, J. M. F.; Martinho, J. M. G.; Cabral, J. M. S.; Palavra, A. M. F.. "Supercritical antisolvent micronization of minocycline hydrochloride". Journal of Supercritical Fluids 44 2 (2008): 238-244. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000254430100014&KeyUID=WOS:000254430100014.
    10.1016/j.supflu.2007.09.035
  79. Martins, S. A. M.; Prazeres, D. M. F.; Fonseca, L. P.; Monteiro, G. A.. "Chemiluminescent bead-based hybridization assay for the detection of genomic DNA from E-coli in purified plasmid samples". Analytical and Bioanalytical Chemistry 391 6 (2008): 2179-2187. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000257200200023&KeyUID=WOS:000257200200023.
    10.1007/s00216-008-2142-5
  80. Ribeiro, Sofia C.; Oliveira, Pedro H.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "High Frequency Plasmid Recombination Mediated by 28 bp Direct Repeats". Molecular Biotechnology 40 3 (2008): 252-260. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000260068300005&KeyUID=WOS:000260068300005.
    10.1007/s12033-008-9082-3
  81. Oliveira, Pedro H.; Lemnos, Francisco; Monteiro, Gabriel A.; Prazeres, Duarte M. F.. "Recombination frequency in plasmid DNA containing direct repeats-predictive correlation with repeat and intervening sequence length". Plasmid 60 2 (2008): 159-165. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000259173500009&KeyUID=WOS:000259173500009.
    10.1016/j.plasmid.2008.06.004
  82. Silva, MS; Martinez-Salas, E; Lanca, AS; De Sousa, KP; Ferrao, C; Monteiro, GA. "Bicistronic Plasmid Approach for DNA Vaccine Development Against African Trypanosomiasis". Vaccine (2008): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000268750600185&KeyUID=WOS:000268750600185.
  83. Azzoni, Adriano R.; Ribeiro, Sofia C.; Monteiro, Gabriel A.; Prazeres, Duarte M. F.. "The impact of polyadenylation signals on plasmid nuclease-resistance and transgene expression". Journal of Gene Medicine 9 5 (2007): 392-402. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000247051100008&KeyUID=WOS:000247051100008.
    10.1002/jgm.1031
  84. Carapuca, Elisabete; Azzoni, Adriano R.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.; Mergulhao, Filipe J. M.. "Time-course determination of plasmid content in eukaryotic and prokaryotic cells using Real-Time PCR". Molecular Biotechnology 37 2 (2007): 120-126. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000249766800003&KeyUID=WOS:000249766800003.
    10.1007/s12033-007-0007-3
  85. Freitas, S. S.; Azzoni, A. R.; Santos, J. A. L.; Monteiro, G. A.; Prazeres, D. M. F.. "On the stability of plasmid DNA vectors during cell culture and purification". Molecular Biotechnology 36 2 (2007): 151-158. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000247271300008&KeyUID=WOS:000247271300008.
    10.1007/s12033-007-0028-y
  86. Mergulhao, Filipe J.; Monteiro, Gabriel A.. "Analysis of factors affecting the periplasmic production of recombinant proteins in Escherichia coli". Journal of Microbiology and Biotechnology 17 8 (2007): 1236-1241. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000249111700002&KeyUID=WOS:000249111700002.
  87. Monteiro, Gabriel António Amaro. "Design and production of DNA vaccines". Bioforum Europe (discontinued, ISSN:1611-597X) 12/07:20-22 (2007): http://www.academia.edu/7520738/DNA_Vaccines_Design_and_Production.
  88. Henriques, Ana M.; Fevereiro, Miguel; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Development of a candidate DNA vaccine against Maedi-Visna virus". Veterinary Immunology and Immunopathology 119 3-4 (2007): 222-232. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000250022200007&KeyUID=WOS:000250022200007.
    10.1016/j.vetimm.2007.05.004
  89. Wu, Ming Liang; Prazeres, Duarte; Monteiro, Gabriel; Santos, Jose. "Stabilization of plasmid DNA against mechanical damage". Journal of Biotechnology 131 2 (2007): S189-S189. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000249687100324&KeyUID=WOS:000249687100324.
    10.1016/j.jbiotec.2007.07.336
  90. Carvalho, Joana; Azzoni, Adriano R.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Targeting antigens to the major pathways of presentation and processing of antigens: A strategy to obtain improved DNA vaccines". Human Gene Therapy 18 10 (2007): 1057-1057. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000250339500306&KeyUID=WOS:000250339500306.
  91. Oliveira, PHC; Nunes, SC; Ribeiro, SC; Prazeres, DMF; Monteiro, GA. "Kanamicin-induced recombination in E-coli cells harbouring plasmids with direct repeats". Febs Journal 274 (2007): 195-195. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000253283800603&KeyUID=WOS:000253283800603.
  92. Azzoni, Adriano R.; Beira, Jorge; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Replacement of nuclease labile sequences in the origin of replication of plasmid vectors can significantly improve transgene expression". Human Gene Therapy 18 10 (2007): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000250339500294&KeyUID=WOS:000250339500294.
  93. Azzoni, Adriano R.; Wu, Ming L.; Freitas, Sindelia S.; Monteiro, Gabriel A.; Prazeres, Duarte M. F.. "Gene delivery using non-viral vectors: Trace impurities in plasmid DNA preparations can strongly affect transfection of mammalian cells". Human Gene Therapy 18 10 (2007): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000250339500295&KeyUID=WOS:000250339500295.
  94. Valente, C. A.; Monteiro, G. A.; Cabral, J. M. S.; Fevereiro, M.; Prazeres, D. M. F.. "Optimization of the primary recovery of human interferon alpha 2b from Escherichia coli inclusion bodies". Protein Expression and Purification 45 1 (2006): 226-234. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000234521100028&KeyUID=WOS:000234521100028.
    10.1016/j.pep.2005.06.014
  95. Passarinha, L. A.; Diogo, M. M.; Queiroz, J. A.; Monteiro, G. A.; Fonseca, L. P.; Prazeres, D. M. F.; Passarinha, L.A.; et al. "Production of ColE1 type plasmid by Escherichia coli DH5 alpha cultured under nonselective conditions". Journal of Microbiology and Biotechnology 16 1 (2006): 20-24. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000234980800003&KeyUID=WOS:000234980800003.
  96. Silva, MS; Monteiro, GA; Atouguia, J; Liberato, P; Prazeres, DMF. "Humoral immune response induced in outbred CD1 mice by DNA vaccine against African Trypanosomiasis". Icopa Xi: Proceedings of the 11th International Congress of Parasitology (2006): 597-602. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000243529300106&KeyUID=WOS:000243529300106.
  97. Ribeiro, SC; Oliveira, PH; Prazeres, DMF; Monteiro, GA. "Kanamicin-induced adaptive mutation in E. coli cells harbouring plasmids with direct repeats". Febs Journal 273 (2006): 50-50. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000238914000164&KeyUID=WOS:000238914000164.
  98. Carvalho, J; Silva, M; Atouguia, J; Prazeres, M; Monteiro, G. "Targeting of antigen to the secretory and endocytic pathway: development of a vaccine against sleeping sickness". Febs Journal 273 (2006): 69-69. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000238914000230&KeyUID=WOS:000238914000230.
  99. Mergulhao, F. J. M.; Summers, D. K.; Monteiro, G. A.. "Recombinant protein secretion in Escherichia coli". Biotechnology Advances 23 3 (2005): 177-202. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000228314600001&KeyUID=WOS:000228314600001.
    10.1016/j.biotechadv.2004.11.003
  100. Azzoni, A. R.; Tavares, A.; Monteiro, G. A.; Prazeres, D. M. F.. "Overcoming the nuclease barrier to gene expression during trafficking of plasmid DNA vectors". Journal of Biotechnology 118 (2005): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000231195200249&KeyUID=WOS:000231195200249.
  101. Mergulhao, FJM; Monteiro, GA. "The secretion efficiency of the Escherichia coli Sec pathway is dependent on the growth phase but not on protein size". Journal of Biotechnology 118 (2005): S109-S109. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000231195200378&KeyUID=WOS:000231195200378.
  102. Valente, C. A.; Prazeres, D. M. F.; Cabral, J. M. S.; Monteiro, G. A.. "Translational features of human alpha 2b interferon production in Escherichia coli". Applied and Environmental Microbiology 70 8 (2004): 5033-5036. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000223290100080&KeyUID=WOS:000223290100080.
    10.1128/aem.70.8.5033-5036.2004
  103. Mergulhao, F. J. M.; Taipa, M. A.; Cabral, J. M. S.; Monteiro, G. A.. "Evaluation of bottlenecks in proinsulin secretion by Escherichia coli". Journal of Biotechnology 109 1-2 (2004): 31-43. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000220934300005&KeyUID=WOS:000220934300005.
    10.1016/j.jbiotec.2003.10.024
  104. Mergulhao, F. J. M.; Monteiro, G. A.. "Secretion capacity limitations of the Sec pathway in Escherichia coli". Journal of Microbiology and Biotechnology 14 1 (2004): 128-133. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000189267900019&KeyUID=WOS:000189267900019.
  105. Ribeiro, S. C.; Monteiro, G. A.; Prazeres, D. M. F.. "The role of polyadenytation signal secondary structures on the resistance of plasmid vectors to nucleases". Journal of Gene Medicine 6 5 (2004): 565-573. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000221654100008&KeyUID=WOS:000221654100008.
    10.1002/jgm.536
  106. Mergulhão, F.J.M.; Monteiro, G.A.; Cabral, J.M.S.; Taipa, M.A.; Mergulhao, F. J. M.; Monteiro, G. A.; Cabral, J. M. S.; Taipa, M. A.. "Design of bacterial vector systems for the production of recombinant proteins in Escherichia coli". Journal of Microbiology and Biotechnology 14 1 (2004): 1-14. http://www.scopus.com/inward/record.url?eid=2-s2.0-1542285371&partnerID=MN8TOARS.
  107. Martins, S. A. M.; Prazeres, D. M. F.; Cabral, J. M. S.; Monteiro, G. A.. "Comparison of real-time polymerase chain reaction and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products". Analytical Biochemistry 322 1 (2003): 127-129. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000186008800016&KeyUID=WOS:000186008800016.
    10.1016/j.ab.2003.07.004
  108. Mergulhao, F. J. M.; Monteiro, G. A.; Larsson, G.; Bostrom, M.; Farewell, A.; Nystrom, T.; Cabral, J. M. S.; Taipa, M. A.. "Evaluation of inducible promoters on the secretion of a ZZ-proinsulin fusion protein in Escherichia coli". Biotechnology and Applied Biochemistry 38 (2003): 87-93. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000184671200012&KeyUID=WOS:000184671200012.
    10.1042/ba20030043
  109. Mergulhao, F. J. M.; Monteiro, G. A.; Larsson, G.; Sanden, A. M.; Farewell, A.; Nystrom, T.; Cabral, J. M. S.; Taipa, M. A.. "Medium and copy number effects on the secretion of human proinsulin in Escherichia coli using the universal stress promoters uspA and uspB". Applied Microbiology and Biotechnology 61 5-6 (2003): 495-501. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000183861300016&KeyUID=WOS:000183861300016.
    10.1007/s00253-003-1232-8
  110. Ribeiro, S. C.; Monteiro, G. A.; Cabral, J. M. S.; Prazeres, D. M. F.. "Isolation of plasmid DNA from cell lysates by aqueous two-phase systems". Biotechnology and Bioengineering 78 4 (2002): 376-384. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000175353900003&KeyUID=WOS:000175353900003.
    10.1002/bit.10227
  111. Mergulhao, F. J. M.; Monteiro, G. A.; Cabral, J. M. S.; Taipa, M. A.. "A quantitative ELISA for monitoring the secretion of ZZ-fusion proteins using SpA domain as immunodetection reporter system". Molecular Biotechnology 19 3 (2001): 239-244. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000172109800002&KeyUID=WOS:000172109800002.
    10.1385/mb:19:3:239
  112. Diogo, M. M.; Ribeiro, S. C.; Queiroz, J. A.; Monteiro, G. A.; Tordo, N.; Perrin, P.; Prazeres, D. M. F.. "Production, purification and analysis of an experimental DNA vaccine against rabies". Journal of Gene Medicine 3 6 (2001): 577-584. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000172871600006&KeyUID=WOS:000172871600006.
    10.1002/jgm.218
  113. Prazeres, D. M.; Monteiro, G. A.; Ferreira, G. N.; Diogo, M. M.; Ribeiro, S. C.; Cabral, J. M.. "Purification of plasmids for gene therapy and DNA vaccination". Biotechnology annual review 7 (2001): 1-30. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:11686041&KeyUID=MEDLINE:11686041.
    10.1016/s1387-2656(01)07031-4
  114. Diogo, M. M.; Ribeiro, S.; Queiroz, J. A.; Monteiro, G. A.; Perrin, P.; Tordo, N.; Prazeres, D. M. F.. "Scale-up of hydrophobic interaction chromatography for the purification of a DNA vaccine against rabies". Biotechnology Letters 22 17 (2000): 1397-1400. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000089338500009&KeyUID=WOS:000089338500009.
    10.1023/a:1005665003375
  115. Ferreira, G. N. M.; Monteiro, G. A.; Prazeres, D. M. F.; Cabral, J. M. S.. "Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications". Trends in Biotechnology 18 9 (2000): 380-388. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000089000700006&KeyUID=WOS:000089000700006.
    10.1016/s0167-7799(00)01475-x
  116. Diogo, M. M.; Queiroz, J. A.; Monteiro, G. A.; Martins, S. A. M.; Ferreira, G. N. M.; Prazeres, D. M. F.. "Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography". Biotechnology and Bioengineering 68 5 (2000): 576-583. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000086928600013&KeyUID=WOS:000086928600013.
    10.1002/(sici)1097-0290(20000605)68:5<576::aid-bit13>3.0.co;2-5
  117. Ribeiro, S. C.; Monteiro, G. A.; Martinho, G.; Cabral, J. M. S.; Prazeres, D. M. F.. "Quantitation of plasmid DNA in aqueous two-phase systems by fluorescence analysis". Biotechnology Letters 22 13 (2000): 1101-1104. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000088035000012&KeyUID=WOS:000088035000012.
    10.1023/a:1005610113677
  118. Mergulhao, F. J. M.; Monteiro, G. A.; Kelly, A. G.; Taipa, M. A.; Joaquim, M. S. C.. "Recombinant human proinsulin: A new approach in gene assembly and protein expression". Journal of Microbiology and Biotechnology 10 5 (2000): 690-693. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000165095600019&KeyUID=WOS:000165095600019.
  119. Monteiro, G. A.; Ferreira, G. N. M.; Cabral, J. M. S.; Prazeres, D. M. F.. "Analysis and use of endogenous nuclease activities in Escherichia coli lysates during the primary isolation of plasmids for gene therapy". Biotechnology and Bioengineering 66 3 (1999): 189-194. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000084141100007&KeyUID=WOS:000084141100007.
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    10.1016/s0167-7799(98)01291-8
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    10.1006/abio.1999.4297
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Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2021/01 - Current The use of Escherichia coli for biofilms for the production of recombinante proteins and other added-value compounds
Co-supervisor
 Doutoramento em Engenharia Química e Biológica (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal
2019/09 - Current Mesenchymal Stromal Cells (MSC)-derived extracellular vesicles for the delivery of RNAi to modulate angiogenesis in malignancy
Supervisor
 Biotecnologia e Biociências (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/12 - 2021/07 Endosomal dynamics at the host-plasmodium interface through liver stage parasite development
Co-supervisor
 Bioengenharia (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/09 - 2021/05 CRISPR/Cas9-based engineering of Escherichia coli for BioBrick based minicircles
Supervisor
 Biotecnologia (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2020/03 - 2020/10 Development of an oral pDNA vaccine against Vibrio species
Co-supervisor
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019 - 2019/11 CRISPR/Cas9-Based Genome Editing Of Lactic Acid Bacteria For The Improvement Of Biopharmaceutical-Grade Plasmid And Recombinant Protein Production
Co-supervisor of José Miguel Miquelão Santos
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019 - 2019/11 Lactic acid bacteria as producers of pharmaceuticalgrade molecules: genetic engineering tools for improvement of Lactococcus lactis strains
Co-supervisor of Rita Pereira Coutinho Relvas Pires
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018 - 2018/11 RNAi as a tool to inhibit the angiogenic potential of human Mesenchymal Stem/Stromal cells in malignancy
Co-supervisor of Cristiana da Silva Ulpiano
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018 - 2018/11 Synthesis and characterization of nanoparticles as DNA vaccine carriers for fish immunization
Co-supervisor of Martim de Sousa Gil Cardeira
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2013/09 - 2018/04 Heterologous production of curcumin by probiotic lactic acid bacteria towards gastrointestinal cancer therapy
Supervisor of Sofia de Oliveira Dias Duarte
 Biotecnologia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
2011/09 - 2017/12 Prototyping a Spintronics Lab-on-Chip system for cancer liquid biopsy analysis
Co-supervisor of Tomás Miguel de Freitas Dias
 Bioengenharia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
2016 - 2017/03 Genomic deletions in Escherichia coli K-12 MG1655 for plasmid DNA production
Supervisor of Diana Tamára Vaz Cipriano
 Microbiologia Aplicada (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2017 - 2017 Estudos sobre o síndrome de Hutchinson-Gilford
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 - 2017 Genome Editing via CRISPR/Cas9 Targeted Integration in CHO cells
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 - 2017 Avian Reovirus: Development of ELISA and Serum Neutralization Tests and Preliminary Gene Cloning for the Development of a Virus-Like Particle Vaccine
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 - 2017 Improvement of biopharmaceutical-grade plasmid production by targeted genome editing of Escherichia coli
Supervisor of Marisa Mariano Faustino
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 - 2017 Synthetic biology based assembly of new plasmids for Lactic Acid Bacteria
Supervisor of Carolina Filipa Fernandes Gonçalves
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 - 2017 Seroprevalence of Schmallenberg virus antibodies in cattle, Portugal, 2017
Co-supervisor of Beatriz Rocha Tavares
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 - 2017 Evaluation of a Genome Editing Approach in Lactic Acid Bacteria Based on the CRISPR/Cas9 System
Supervisor of Inês da Costa Gonçalves
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 - 2017 Lactic acid bacteria as cell factories: A synthetic biology approach for plasmid DNA and recombinant protein production
Supervisor
 Biotecnologia e Biociências (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 - 2016 Caracterização imunoquímica de antigénios com reatividade serológica cruzada em tripanosomatídeos (Trypanosoma brucei, Trypanosoma cruzi e Leishmania spp)
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 - 2016 Alternative splicing during neuronal development
Co-supervisor
 Engenharia Biomédica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 - 2016 Flexible insect cell platforms for fast production of pseudotyped virus-like particles
Co-supervisor
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 - 2016 Engineering bacterial cells for the redox neutral conversion of alcohols into optically active amines
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 - 2016 Construction of Virus-like Particles (VLPs) for the Development of Alternative Vaccines against Maedi-Visna Virus (MVV) and Caprine Arthritis-Encephalitis Virus (CAEV)
Co-supervisor
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 - 2016 Efeito da presença de finos na bio-cimentação de solos
Co-supervisor of Inês Mendes Pires
 Engenharia Civil (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 - 2016 Cloning and overexpression of the CaiC protein from the caiTABCDE operon involved in the biosynthesis of L-carnitine in Escherichia coli
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 - 2016 Studies on the uptake of olivetol and olivetolic acid by Saccharomyces cerevisiae
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 - 2016 Produção de proteínas membranares de Vibrio alginolyticus para vacinação
Co-supervisor of Ayéwa Lawoé Agognon
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2010/09 - 2015/01 Strategies to increase DNA vaccines resistance to nucleases
Supervisor of Salomé Alexandra de Sá Magalhães
 Biotecnologia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
2015 - 2015 Reconstitution of Ion Channels in a Lipid Bilayer in a Microfluidic Device using Cell-Free Protein Synthesis
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015 - 2015 Purification of PaaK and PaaY from Escherichia coli: Study of the possible points of acetylation of these proteins involved in the metabolism of phenylacetic acid degradation
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015 - 2015 Reverse lipofection for spatially defined transfection with DNA/cationic lipoplexes
Supervisor of Ana Inês Botelho de Gomes Meleiro
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015 - 2015 Redesign of Lactic Acid Bacteria for plasmid DNA production - a strategy for increase the plasmid copy number
Supervisor of Maria Santos Carlos Martins
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014 - 2014/12 Development of a method for the purification of minicircles
Co-supervisor of Cláudia Patrícia Almeida Alves
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2013 - 2014/12 Production of therapeutic plasmid DNA by lactic acid bacteria
Supervisor of Sílvia Marina de Jesus Andrade
 Biologia Molecular e Genética (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2014 - 2014/11 Construction of Escherichia coli strains for higher plasmid production: Evaluation of lon gene knockout and implementation of a marker-free system
Supervisor of Diana Sofia Cardoso Marques
Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014 - 2014/11 Bio-cimentação de solos arenosos para melhoramento das suas características hidro-mecânicas
Co-supervisor of Rita Romeu Pedreira
 Engenharia Civil (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2013 - 2014/06 Design and production of sílica and ORMOSIL nanoparticles for gene delivery
Co-supervisor of Joana Catarina Capinha de Matos
 Ciências de Engenharia - Engenharia Biomédica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014 - 2014 Construction of plasmids for Schmallenberg virus proteins expression
Co-supervisor
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014 - 2014 Assessment of cell response to microcarriers biofunctionalisation with a bi-functional fusion protein
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014 - 2014 The impact of zwf overexpression on plasmid biopharmaceutical production by Escherichia coli
Supervisor of Rita Azevedo Fernandes
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014 - 2014 Minicircle production and delivery to human mesenchymal stem/stromal cells for angiogenesis stimulation
Supervisor of Liliana Isabel Casimiro Brito
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014 - 2014 Construction of Escherichia coli strains for higher plasmid production: Evaluation of lon gene knockout and implementation of a marker-free system
Supervisor
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014 - 2014 Cell stiffness assessment based on nuclei morphological response to patterned surfaces
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2010/09 - 2013/12 Development of a process for the production and purification of minicircles for biopharmaceutical applications
Supervisor of Michaela Simcikova
 Bioengenharia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
2009/03 - 2013/12 Impact of plasmid structure and downstream processing in transient transfection
Co-supervisor of Jonathan De la Vega Olivas
 Biotecnologia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
2013 - 2013/10 Biomimetic replicas. Transfer of complex architectures from plant surfaces onto polymeric materias
Supervisor of Pedro Miguel Matos Pereira
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2009/09 - 2013/04 Rational engineering of E. coli strains and vectors for improved manufacturing of plasmid biopharmaceuticals
Co-supervisor of Geisa Aparecida Gonçalves
 Bioengenharia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
2013 - 2013 Improvement of Avian Influenza DNA vaccine candidates: The impact of antigen-targeting sequences
Supervisor of Margarida Susana Barradas dos Santos Azevedo
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2013 - 2013 Evaluation of rpiA promoter strength in plasmid DNA production
Supervisor of Luís António Menezes Carreira
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2012 - 2012/11 Non-viral engineered human mesenchymal stem/stromal cells to promote angiogenesis
Co-supervisor of Teresa Filipa Oliveira Trindade Pereira
 Ciências de Engenharia - Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2012 - 2012 Construção do clone molecular infeccioso do isolado P1OLV do vírus Maedi-Visna
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2012 - 2012 Non-invasive measurement of steroid hormones in zebrafish holding water
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2012 - 2012 DNA vaccines against avian influenza virus: Enhancing immune response by protein targeting
Supervisor of Elisabete de Figueiredo Quintas Borges de Freitas
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2012 - 2012 Characterization of the coelomic fluid of the starfish Marthasterias glacialis in a wound-healing phase
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2012 - 2012 Purification of Plasmid Minicircles Biopharmaceuticals by Combined Enzymatic and Chromatographic Techniques
Supervisor of Nadiya Kubasova
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2010 - 2011/06 The role of calcium in Saccharomyces sp. In response to ethanol stress
Supervisor of Sofia de Oliveira Dias Duarte
 Microbiologia Aplicada (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2011 - 2011 Magnetoresistive Chip-based platform for the evaluation of cfDNA integrity as a potential biomarker in cancer diagnosis
Supervisor of Tomás Miguel de Freitas Dias
 Bioengenharia e Nanossistemas (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2011 - 2011 Development of DNA vaccines prototypes against avian influenza viruses
Supervisor of Joana Rita Pires Bentes Gil
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2005/12 - 2010/07 Development of DNA vaccines against African Trypanosomiasis encoding antigen-targeting sequences
Supervisor of Joana de Abreu Carvalho
 Biotecnologia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
2006/01 - 2010/01 Structural instability in plasmid DNA
Supervisor of Pedro Henrique Couto Oliveira
 Biotecnologia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
2010 - 2010 Mineral nanoparticles: uptake and cytotoxicity in CaCo2 cells
Co-supervisor
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2003/01 - 2008/12 Development of quantitative microparticle-based assays for DNA hybridization
Supervisor of Sofia Medina Aires Martins
 Biotecnologia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
2005/06 - 2007/11 Effect of chemical agents on plasmid resistance to nucleases
Supervisor of Ana Luísa Monteiro Garrido Castro e Silva
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2002/03 - 2006/12 Vacinas de DNA: um modelo experimental de imunização contra a tripanossomose africana
Co-supervisor of Marcelo de Sousa da Silva
 Biotecnologia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
2001/01 - 2006/03 Screening for hot spots in plasmids for gene therapy and DNA vaccination
Co-supervisor of Sofia Cabral de Andrade Duarte Ribeiro
 Biotecnologia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
2004/06 - 2006/02 Influência da resposta restringente na produção de plasmídeos em Escherichia coli
Supervisor of Elisabete Florêncio Carapuça
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2004/06 - 2005/12 Direccionamento de antigénios no desenvolvimento de vacinas de DNA contra a Tripanossomose Africana
Supervisor of Joana de Abreu Carvalho
 Biotecnologia
Universidade de Lisboa Instituto Superior Técnico, Portugal
2000/09 - 2005/05 Desenvolvimento de uma vacina de DNA contra o vírus Maedi-Visna dos ovinos
Supervisor of Ana Margarida Ferreira Henriques de Oliveira Mourão
 Biotecnologia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
1998/03 - 2002/07 Design of bacterial vector systems for the secretion of recombinant proteins
Co-supervisor of Filipe José Menezes Mergulhão
 Biotecnologia (PhD)
Universidade de Lisboa Instituto Superior Tecnico, Portugal
2000/06 - 2002/06 Monitorização de DNA genómico ao longo do processo de purificação do plasmídeo pVAX1gag
Supervisor of Sofia Medina Aires Martins
 Biotecnologia
Universidade de Lisboa Instituto Superior Técnico, Portugal
1999/06 - 2001/01 Extracção de DNA plasmídico por sistemas de duas fases aquosas
Supervisor of Sofia Cabral de Andrade Duarte Ribeiro
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
1999 - 1999 Quantificação de DNA genómico presente em preparações puras de plasmídeos para terapia génica
Supervisor of Sofia Medina Aires Martins
 Engenharia Biológica (Degree)
Universidade Lusofona de Humanidades e Tecnologias, Portugal
Distinctions

Other distinction

2010 Menção honrosa UTL/Santander Totta 2010