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Dr. José Bernardo Gama is an Investigator at the CICS-UBI (Health Sciences Research Centre - University of Beira Interior). He has published 9 manuscripts in international peer-reviewed journals, 3 of which as a first or co-first author. His papers have a total of 337 citations, which give him an h-index of 9. Dr. Gama graduated in Biochemistry in 2008, after one full year of internship in the lab of Prof. Ferreira (ICBAS, Porto), where he carried out his senior year research project. He studied, the efficiency and safety of the vaccination trials against Group B Streptococcus (GBS) infection, using the GBS virulence factor, GAPDH, as an antigenic target. In 2009, Dr. Gama was awarded a fellowship from FCT to pursue a PhD in Prof. Pedrosa's lab (ICVS, Braga), where he studied the molecular cytopathogenesis induced by mycolactone, the toxin of Mycobacterium ulcerans. His proteomic analysis revealed that cytoskeleton dynamics and collagen synthesis are processes sensitive to mycolactone (Gama JB et al., PLoS Negl Trop Dis 2014), which was also found to trigger a BIM-mediated apoptosis. After receiving his PhD in 2014, Dr. Gama joined Dr. Gassmann's lab (IBMC, Porto) to dissect the molecular mechanisms used by Dynein-1 adaptors to recruit the motor machinery to the different cargos. His studies contributed to the current understanding of how functionally distinct adaptors with diverse structures use a similar mechanism to engage the molecular motor Dynein-1 and its cofactor dynactin (GAMA JB et al., J Cell Biology 2017; and Celestino R*, Henen MA*, GAMA JB*, et al., PLoS Biology 2019; *: Equal contribution). In 2020, Dr. Gama joined the group of Dr. Tiago Dantas (i3S, Porto), to study how mutations in regulators or subunits of Dynein-1 and Dynein-2 result in severe developmental disorders, such as microcephaly and skeletal ciliopathies, respectively. Since 2021, he is collaborating with Prof. Maia (CICS-UBI), to study the role of STEAP1 on the oncobiology of prostate cancer in order to evaluate its potential as biomarkers and/or therapeutic target.
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Identification

Personal identification

Full name
José Bernardo Costa Gama

Citation names

  • Gama, José Bernardo

Author identifiers

Ciência ID
0518-D7F8-0B75
ORCID iD
0000-0002-7011-1502
Education
Degree Classification
2020 - 2026
Ongoing
 Integrated Master's Degree in Medicine (Licenciatura)
Universidade da Beira Interior Faculdade de Ciências da Saúde, Portugal
2018
Concluded
 Course in Laboratory Animal Science accredited by FELASA following the recommendations for Category C (Outros)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Aprovado
2014
Concluded
 Health sciences (Doutoramento)
Universidade do Minho Escola de Medicina, Portugal
"Molecular cytopathogenesis induced by mycolactone, the Mycobacterium ulcerans toxin" (THESIS/DISSERTATION)
 Aprovado
2008
Concluded
 Ensaios de vacinação em fêmeas BALB/c contra a infecção por Streptococcus agalactiae nos recém-nascidos usando a gliceraldeído-3-fosfato desidrogenase recombinante como antigénio alvo (Outros)
Major in Dissertação estágio
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
 20/20
2008
Concluded
 Biochemistry (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
 15/20
Affiliation

Science

Category
Host institution 		Employer
2021/01 - Current Researcher (Research) Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2020/03 - 2020/10 Contracted Researcher (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019/11 - 2020/02 Contracted Researcher (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2014/11 - 2019/10 Postdoc (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Projects

Grant

Designation Funders
2009/01/01 - 2012/12/31 Molecular cytopathogenesis induced by the Mycobacterium ulcerans toxin, mycolactone
SFRH/BD/33573/2009
PhD Student Fellow
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2020/03/01 - Current Investigating the molecular causes of severe Microcephaly: Primary cilium dependent cell cycle control of neural stem cell proliferation during brain development (POCI grant)
PTDC/BIA-BID/29471/2017
Researcher
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2019/11/01 - 2020/02/29 Molecular mechanisms of the dynein-dynactin transport machinery in health and disease
PTDC/BIA-CEL/30507/2017
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2014/03/01 - 2019/10 Cytoplasmic Dynein: Mechanisms of Regulation and Novel Interactors
info:eu-repo/grantAgreement/EC/FP7/338410/EU
Post-doc Fellow
European Commission
2009/01/01 - 2012/12/31 Úlcera do Buruli - Da compreensão dos mecanismos de resistência do hospedeiro ao desenvolvimento de novas intervenções contra uma micobacteriose emergente
94776
PhD Student Fellow
Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
 Fundação Calouste Gulbenkian
Concluded
Outputs

Publications

Conference poster
  1. Duro, Joana; Gama, José Bernardo; Barbosa, Daniel José; Carvalho, Cátia; Gassmann, Reto. "A C. elegans dynactin p150 mutant with reduced affinity for dynein intermediate chain uncouples dynein recruitment from dynein activation". Paper presented in ASCB|EMBO Annual Meeting, 2018.
  2. Carvalho, Cátia; Celestino, Ricardo; Gama, José Bernardo; Barbosa, Daniel José; Gassmann, Reto. "A short helix in the C-terminal region of dynein light intermediate chain links the motor to structurally diverse adaptors for cargo transport". Paper presented in EMBO Workshop: C. elegans development, cell biology and gene expression, 2018.
Journal article
  1. Ricardo Celestino; José B. Gama; Artur F. Castro-Rodrigues; Daniel J. Barbosa; Helder Rocha; Ennio A. d’Amico; Andrea Musacchio; et al. "JIP3 interacts with dynein and kinesin-1 to regulate bidirectional organelle transport". Journal of Cell Biology (2022): https://doi.org/10.1083/jcb.202110057.
    10.1083/jcb.202110057
  2. Celestino R*; Henen M*; GAMA JB*; Carvalho, C.; McCabe M; Barbosa D; Born A; et al. "A transient helix in the disordered region of dynein light intermediate chain links the motor to structurally diverse adaptors for cargo transport". PLOS Biology 17 1 (2019): e3000100-e3000100. https://doi.org/10.1371/journal.pbio.3000100.
    10.1371/journal.pbio.3000100
  3. Pereira, Cláudia; Reis, Rita M.; GAMA, José B.; Celestino, Ricardo; Cheerambathur, Dhanya K.; Carvalho, Ana X.; Gassmann, Reto. "Self-Assembly of the RZZ Complex into Filaments Drives Kinetochore Expansion in the Absence of Microtubule Attachment". Current Biology 28 21 (2018): 3408-3421.e8. http://dx.doi.org/10.1016/j.cub.2018.08.056.
    10.1016/j.cub.2018.08.056
  4. GAMA , J.B.; Pereira, C.; Simões, P.A.; Celestin, R.; Reis, R.M.; Barbosa, D.J.; Pires, H.R.; et al. "Molecular mechanism of dynein recruitment to kinetochores by the Rod-Zw10-Zwilch complex and Spindly". Journal of Cell Biology 216 4 (2017): 943-960. http://www.scopus.com/inward/record.url?eid=2-s2.0-85021847678&partnerID=MN8TOARS.
    10.1083/jcb.201610108
  5. Cruz, A.; Ludovico, P.; Torrado, E.; GAMA, J.B.; Sousa, J.; Gaifem, J.; Appelberg, R.; et al. "IL-17A promotes intracellular growth of Mycobacterium by inhibiting apoptosis of infected macrophages". Frontiers in Immunology 6 SEP (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84946550863&partnerID=MN8TOARS.
    10.3389/fimmu.2015.00498
  6. GAMA, J.B.; Ohlmeier, S.; Martins, T.G.; Fraga, A.G.; Sampaio-Marques, B.; Carvalho, M.A.; Proença, F.; et al. "Proteomic analysis of the action of the mycobacterium ulcerans toxin Mycolactone: Targeting host cells cytoskeleton and collagen". PLoS Neglected Tropical Diseases 8 8 (2014): 1-14. http://www.scopus.com/inward/record.url?eid=2-s2.0-84939784611&partnerID=MN8TOARS.
    10.1371/journal.pntd.0003066
  7. Martins, T.G.; GAMA, J.B.; Fraga, A.G.; Saraiva, M.; Silva, M.T.; Castro, A.G.; Pedrosa, J.. "Local and regional re-establishment of cellular immunity during curative antibiotherapy of murine mycobacterium ulcerans infection". PLoS ONE 7 2 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84857676432&partnerID=MN8TOARS.
    10.1371/journal.pone.0032740
  8. Martins, T.G.; Trigo, G.; Fraga, A.G.; GAMA, J.B.; Longatto-Filho, A.; Saraiva, M.; Silva, M.T.; Castro, A.G.; Pedrosa, J.. "Corticosteroid-Induced Immunosuppression Ultimately Does Not Compromise the Efficacy of Antibiotherapy in Murine Mycobacterium ulcerans Infection". PLoS Neglected Tropical Diseases 6 11 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84870754979&partnerID=MN8TOARS.
    10.1371/journal.pntd.0001925
  9. Madureira, P.; Andrade, E.B.; GAMA, J.B.; Oliveira, L.; Moreira, S.; Ribeiro, A.; Correia-Neves, M.; et al. "Inhibition of IL-10 production by maternal antibodies against group b streptococcus GAPDH confers immunity to offspring by favoring neutrophil recruitment". PLoS Pathogens 7 11 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-81755177757&partnerID=MN8TOARS.
    10.1371/journal.ppat.1002363
  10. Torrado, E.; Fraga, A.G.; Logarinho, E.; Martins, T.G.; Carmona, J.A.; GAMA, J.B.; Carvalho, M.A.; et al. "IFN-gamma-dependent activation of macrophages during experimental infections by Mycobacterium ulcerans is impaired by the toxin mycolactone". Journal of Immunology 184 2 (2010): 947-955. http://www.scopus.com/inward/record.url?eid=2-s2.0-76249087225&partnerID=MN8TOARS.
    10.4049/jimmunol.0902717
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2020 - Current Uncovering the mechanisms regulating cargo selection for Dynein transport during brain development
Co-supervisor
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019 - 2019 Functional analysis of novel cargo adaptors for the molecular motor cytoplasmic Dynein-1
Co-supervisor
 BIM (Other)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Mentoring / Tutoring

Topic Student name
2011 - 2012 Effect of mycolactone on the expression of Bcl-2 family proteins in Buruli Ulcer's mouse model Nuno Miguel Lopes Araújo
2008/12/01 - 2009/11/30 Estudo do efeito citotóxico da toxina do Micobacterium ulcerans (micolactona) em diferentes linhas celulares Diogo Pinto e Lobo de Jesus Silva

Other jury / evaluation

Activity description Institution / Organization
2017 - Current Reviewer for several journals including Communications Biology from Nature.
Distinctions

Award

2009 PhD fellowship
Fundação para a Ciência e a Tecnologia, Portugal