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Nuno Mendes, BSc, MSc, is a Senior Research Technician and PhD candidate at the Instituto de Inovação e Investigação em Saúde (i3S) at the University of Porto. He obtained his degree as a Pathology Technician from Escola Superior de Saúde do Porto and a Master’s in Medicine and Molecular Oncology from Faculdade de Medicina da Universidade do Porto in 2007. Nuno began his research career at the Instituto de Patologia e Imunologia Molecular da Universidade do Porto (IPATIMUP) in 2003, contributing to projects on gastric pathology linked to Helicobacter pylori infection. In 2009, he was contracted by IPATIMUP to work on cancer models (xenograft and transgenic) and to establish IPATIMUP’s Bank of Cell Lines. With contributions to over 30 publications in peer-reviewed journals, Nuno’s expertise spans animal experimentation, histology, and immunohistochemistry—from experimental design and cancer model development to drug testing and sample characterization. Currently pursuing his PhD, he is also a core member of the Histology and Electron Microscopy Scientific Platform at i3S, where he leads the development of high-throughput multiplex imaging techniques using state-of-the-art PhenoImager HT and Hyperion Tissue Imaging systems.
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Identification

Personal identification

Full name
Nuno Miguel Morais Mendes

Citation names

  • Mendes, Nuno

Author identifiers

Ciência ID
0619-093D-BE86
ORCID iD
0000-0002-0570-2277

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2023/09/01 - 2026/07/01
Ongoing
 Patologia e Genética Molecular (Doutoramento)
Major in Patologia, Medicina, Oncologia,
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2023/09/01 - 2024/06/30
Attended
 Patologia e Genética Molecular (Curso de doutoramento (conclusão de unidades curriculares))
Major in Medicina, Saúde, Oncologia Molecular, Patologia
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
 15/20
2015/03/30 - 2015/04/01
Concluded
 Training School in the Experimental Design and Statistical Analysis of Biomedical Experiments (Pós-Graduação)
Major in Experimentação biomédica em modelos animais
CNC.IBILI, Portugal
 Aprovado
2009/03/01 - 2009/03/31
Concluded
 Professional Specialization in Animal Models Science, Category C according to FELASA (Pós-Graduação)
Major in Experimentação biomédica em modelos animais
Universidade do Porto, Portugal
 Aprovado
2008
Concluded
 Medicina e Oncologia Molecular (Mestrado)
Universidade do Porto Faculdade de Medicina, Portugal
""Polymorphisms in the CDX2 gene locus in Portugal and Mozambique. Relation with the development of gastric intestinal metaplasia!" (THESIS/DISSERTATION)
 Muito bom
2005/01/01 - 2007/12/15
Concluded
 Medicina e Oncologia Molecular (Mestrado)
Major in Cancro - modelos de experimentação animal, modelos in vitro, imunohistoquímica
Universidade do Porto Faculdade de Medicina, Portugal
 Muito Bom
2001
Concluded
 Anatomia Patológica, Citológica e Tanatológica (Licenciatura)
Instituto Politécnico do Porto Escola Superior de Saúde, Portugal
 15/20
Affiliation

Others

Category
Host institution 		Employer
2009/02/01 - Current Técnico de Investigação Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
1999/04/01 - Current Técnico de Anatomia Patológica Hospital de Santa Maria, Portugal
2003/01/31 - 2009/01/31 Molecular Pathology Technician at Carcinogenesis Group Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
2000/01/01 - 2004/04/30 Technician of Pathology Centro Hospitalar Universitário de São João, Portugal
Projects

Contract

Designation Funders
2018/07/01 - 2022/06/30 Identificação de Novas Drogas Glicoespecíficos para Combater a Metástase de Células Cancerígenas / Identification of Novel GlycoSpecific Drugs to Tackle Cancer Cell Metastasis
PTDC/MED-QUI/29780/2017
Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Ongoing
2019/03/15 - 2022/03/22 Metastização Cerebral: o impacto molecular e biomecânico das células tumorais no microambiente cerebral / BRAIN METastaseS: uncovering the biomechanical crosstalk between cancer cells and the brain microenvironment
PTDC/MED-ONC/30625/2017
Technical development
Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Ongoing
2018/10/15 - 2021/10/14 MAGICIAM: um sistema imunomodelador de macrófagos para prevenir a invasão e metastização das células tumorais / MAGICIAM: a MAcrophaGe Immunomodulatory-delivery system to prevent Cancer Invasion And Metastasis
PTDC/BTM-SAL/31859/2017
Researcher
Instituto Nacional de Engenharia Biomédica, Portugal

Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Ongoing
2018/09/01 - 2021/08/31 Combate aos cancros metastáticos: o palco é do ruténio; Fighting metastatic cancers: prime time for ruthenium
PTDC/QUI-QIN/28662/2017
Researcher
Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal

Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal
Associação para a Inovação e Desenvolvimento da FCT
Ongoing
2018/09/01 - 2021/08/30 Nanopartículas Multifuncionais para Quimioradioterapia do Glioblastoma Multifunctional / Multifunctional Nanoseeds for Chemoradiotherapy of Glioblastoma
PTDC/MED-QUI/29649/2017
Researcher
Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Ongoing
2018/07/18 - 2021/07/21 Avaliação pré-clínica de novos compostos químicos para o tratamento do cancro: colmatando necessidades clínicas atuais. Pre-clinical evaluation of novel chemical compounds for cancer treatment: tackling unmet clinical needs
PTDC/MED-ONC/31354/2017
Technical development
Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Ongoing
2018/07/15 - 2021/07/14 Previsão da resposta dos pacientes a fármacos antineoplásicos lipossomais: focando na atividade endocítica do LRP1B /Predicting patients' response to liposomal anticancer drugs: focusing on LRP1B endocytic activity
PTDC/MEC-ONC/31520/2017
Technical development
Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Outputs

Publications

Book chapter
  1. Mendes, N.; Dias Carvalho, P.; Martins, F.; Mendonça, S.; Malheiro, A. R.; Ribeiro, A.; Carvalho, J.; Velho, S.. "Animal Models to Study Cancer and Its Microenvironment". In Advances in Experimental Medicine and Biology, 389-401. Springer International Publishing, 2020.
    Published • 10.1007/978-3-030-34025-4_20
Journal article
  1. Carolina Noronha; Ana Sofia Ribeiro; Rita Carvalho; Nuno Mendes; Joaquim Reis; Claudia C. Faria; Ricardo Taipa; Joana Paredes. "Cadherin Expression Profiles Define Glioblastoma Differentiation and Patient Prognosis". Cancers (2024): https://doi.org/10.3390/cancers16132298.
    10.3390/cancers16132298
  2. Tiago Bordeira Gaspar; Sofia Macedo; Ana Sá; Mariana Alves Soares; Daniela Ferreira Rodrigues; Mafalda Sousa; Nuno Mendes; et al. "Characterisation of an Atrx Conditional Knockout Mouse Model: Atrx Loss Causes Endocrine Dysfunction Rather Than Pancreatic Neuroendocrine Tumour". Cancers (2022): https://doi.org/10.3390/cancers14163865.
    10.3390/cancers14163865
  3. Mafalda Santos; Marta Ferreira; Patrícia Oliveira; Nuno Mendes; Ana André; André F. Vieira; Joana B. Nunes; et al. "Epithelial-Mesenchymal Plasticity Induced by Discontinuous Exposure to TGFß1 Promotes Tumour Growth". Biology 11 7 (2022): 1046-1046. https://doi.org/10.3390/biology11071046.
    10.3390/biology11071046
  4. Leonor Côrte-Real; Ana Rita Brás; Adhan Pilon; Nuno Mendes; Ana Sofia Ribeiro; Tiago D. Martins; José Paulo S. Farinha; et al. "Biotinylated Polymer-Ruthenium Conjugates: In Vitro and In Vivo Studies in a Triple-Negative Breast Cancer Model". Pharmaceutics (2022): https://doi.org/10.3390/pharmaceutics14071388.
    10.3390/pharmaceutics14071388
  5. Ricardo Coelho; Sara Ricardo; Ana Luísa Amaral; Yen-Lin Huang; Mariana Nunes; José Pedro Neves; Nuno Mendes; et al. "Regulation of invasion and peritoneal dissemination of ovarian cancer by mesothelin manipulation". Oncogenesis 9 6 (2020): https://doi.org/10.1038/s41389-020-00246-2.
    10.1038/s41389-020-00246-2
  6. Mendes, Nuno. "MEX3A regulates Lgr5 + stem cell maintenance in the developing intestinal epithelium". EMBO reports (2020): http://dx.doi.org/10.15252/embr.201948938.
    10.15252/embr.201948938
  7. Carla Pereira; Daniel Ferreira; Nuno Mendes; Pedro L. Granja; Gabriela M. Almeida; Carla Oliveira. "Expression of CD44v6-Containing Isoforms Influences Cisplatin Response in Gastric Cancer Cells". Cancers (2020): https://doi.org/10.3390/cancers12040858.
    10.3390/cancers12040858
  8. Raquel T. Lima; Diana Sousa; Ana Sara Gomes; Nuno Mendes; Rune Matthiesen; Madalena Pedro; Franklim Marques; et al. "The Antitumor Activity of a Lead Thioxanthone is Associated with Alterations in Cholesterol Localization". Molecules (2018): http://www.mdpi.com/1420-3049/23/12/3301.
    10.3390/molecules23123301
  9. Mendes, Nuno. "SRC inhibition prevents P-cadherin mediated signaling and function in basal-like breast cancer cells". Cell Communication and Signaling (2018): http://dx.doi.org/10.1186/s12964-018-0286-2.
    10.1186/s12964-018-0286-2
  10. Mendes N; Tortosa F; Valente A; Marques F; Matos A; Morais TS; Tomaz AI; Gärtner F; Garcia MH. "In vivo Performance of a Ruthenium-cyclopentadienyl Compound in an Orthotopic 1 Triple Negative Breast Cancer Model.". (2016): http://europepmc.org/abstract/med/27671310.
  11. Mendes, Nuno. "Anti-influenza neuraminidase inhibitor oseltamivir phosphate induces canine mammary cancer cell aggressiveness". PLoS ONE 10 4 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84929484710&partnerID=40&md5=391dc8921d911ca89e34859369871a3c.
    10.1371/journal.pone.0121590
  12. Vieira AF; Ribeiro AS; Dionísio MR; Sousa B; Nobre AR; Albergaria A; Santiago-Gómez A; et al. "P-cadherin signals through the laminin receptor a6ß4 integrin to induce stem cell and invasive properties in basal-like breast cancer cells.". (2014): http://europepmc.org/abstract/med/24553076.
  13. Mendes, Nuno. "Morphological features and mucin expression profile of breast carcinomas with signet-ring cell differentiation". Pathology Research and Practice (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84930921359&partnerID=40&md5=03969141feef9e601b21dda0b7da797e.
    10.1016/j.prp.2015.05.003
  14. Mendes, Nuno. "P-cadherin functional role is dependent on E-cadherin cellular context: A proof of concept using the breast cancer model". Journal of Pathology 229 5 (2013): 705-718. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875054847&partnerID=40&md5=14115a793b40138dda5448c0dec9ac46.
    10.1002/path.4143
  15. Mendes, Nuno. "P-cadherin is coexpressed with CD44 and CD49f and mediates stem cell properties in basal-like breast cancer". Stem Cells 30 5 (2012): 854-864. http://www.scopus.com/inward/record.url?eid=2-s2.0-84860499799&partnerID=40&md5=77c36be0903c6e9003aed90d35248e41.
    10.1002/stem.1075
  16. Mendes, Nuno. "Association between environmental factors and CDX2 expression in gastric cancer patients". European Journal of Cancer Prevention 21 5 (2012): 423-431. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864358456&partnerID=40&md5=12fd68cb01506c960e20d8df2af3c21d.
    10.1097/CEJ.0b013e32834ef1aa
  17. Mendes, Nuno. "Differential expression of a-2,3-sialyltransferases and a-1,3/4-fucosyltransferases regulates the levels of sialyl Lewis a and sialyl Lewis x in gastrointestinal carcinoma cells". International Journal of Biochemistry and Cell Biology 42 1 (2010): 80-89. http://www.scopus.com/inward/record.url?eid=2-s2.0-70649087034&partnerID=40&md5=f674e92a2a23bbacbfc7ff9c9dd01db7.
    10.1016/j.biocel.2009.09.010
  18. Mendes, Nuno. "Fut2-null mice display an altered glycosylation profile and impaired BabA-mediated Helicobacter pylori adhesion to gastric mucosa". Glycobiology 19 12 (2009): 1525-1536. http://www.scopus.com/inward/record.url?eid=2-s2.0-71049182586&partnerID=40&md5=f51e9a8b6705129dec9e825fd2619912.
    10.1093/glycob/cwp131
  19. Mendes, Nuno. "Juvenile polyps have gastric differentiation with MUC5AC expression and downregulation of CDX2 and SMAD4". Histochemistry and Cell Biology 131 6 (2009): 765-772. http://www.scopus.com/inward/record.url?eid=2-s2.0-67349211762&partnerID=40&md5=d01b2e8f6ff027d75336b9d22265e187.
    10.1007/s00418-009-0579-z
  20. Mendes, Nuno. "Mixed lineage kinase 3 gene mutations in mismatch repair deficient gastrointestinal tumours". Human Molecular Genetics 19 4 (2009): 697-706. http://www.scopus.com/inward/record.url?eid=2-s2.0-77950353937&partnerID=40&md5=26dd3984e26151acc24dd3d253313be3.
    10.1093/hmg/ddp536
  21. Mendes, Nuno. "Relevance of MUC1 mucin variable number of tandem repeats polymorphism in Hpylori adhesion to gastric epithelial cells". World Journal of Gastroenterology 14 9 (2008): 1411-1414. http://www.scopus.com/inward/record.url?eid=2-s2.0-41149171821&partnerID=40&md5=fd1cc6864675451d607e1171267d59c3.
    10.3748/wjg.14.1411
  22. Mendes, Nuno. "Infection by Helicobacter pylori expressing the BabA adhesin is influenced by the secretor phenotype". Journal of Pathology 215 3 (2008): 308-316. http://www.scopus.com/inward/record.url?eid=2-s2.0-46849109568&partnerID=40&md5=46f181c4a8b2e7b5350bbb2c73f4d802.
    10.1002/path.2363
  23. Mendes, Nuno. "Key elements of the BMP/SMAD pathway co-localize with CDX2 in intestinal metaplasia and regulate CDX2 expression in human gastric cell lines". Journal of Pathology 215 4 (2008): 411-420. http://www.scopus.com/inward/record.url?eid=2-s2.0-49449092484&partnerID=40&md5=6d185aad2d43e85d5b001f7a2ed4b82c.
    10.1002/path.2369
  24. Mendes, Nuno. "Helicobacter pylori induces ß3GnT5 in human gastric cell lines, modulating expression of the SabA ligand sialyl-Lewis x". Journal of Clinical Investigation 118 6 (2008): 2325-2336. http://www.scopus.com/inward/record.url?eid=2-s2.0-45649085812&partnerID=40&md5=07589d88fdd22b6638f0e7de1f7236fd.
    10.1172/JCI34324
  25. Mendes, Nuno. "Expression of Lea in gastric cancer cell lines depends on FUT3 expression regulated by promoter methylation". Cancer Letters 242 2 (2006): 191-197. http://www.scopus.com/inward/record.url?eid=2-s2.0-33749246714&partnerID=40&md5=8be07654ca7cd363495c127c88d39e55.
    10.1016/j.canlet.2005.11.009
  26. Mendes, Nuno. "OCT-1 is over-expressed in intestinal metaplasia and intestinal gastric carcinomas and binds to, but does not transactivate, CDX2 in gastric cells". Journal of Pathology 207 4 (2005): 396-401. http://www.scopus.com/inward/record.url?eid=2-s2.0-29744467037&partnerID=40&md5=bd3771aac808fb6e0f2bc0429b54f59f.
    10.1002/path.1861
  27. Mendes, Nuno. "Distribution of HPV infection and tumour markers in cervical intraepithelial neoplasia from cone biopsies of Mozambican women". Journal of Clinical Pathology 58 1 (2005): 61-68. http://www.scopus.com/inward/record.url?eid=2-s2.0-11244292359&partnerID=40&md5=dd22cf77d400caa4d83363af7eee341d.
    10.1136/jcp.2004.020552
  28. Mendes, Nuno. "Two new FUT2 (fucosyltransferase 2 gene) missense polymorphisms, 739G ¿ A and 839T ¿ C, are partly responsible for non-secretor status in a Caucasian population from Northern Portugal". Biochemical Journal 383 3 (2004): 469-474. http://www.scopus.com/inward/record.url?eid=2-s2.0-9144251105&partnerID=40&md5=89566f63dfb827a1c655e0beee0464e7.
    10.1042/BJ20040803
  29. Mendes, Nuno. "Lewis and Secretor status and Helicobacter pylori eradication (multiple letters) [1]". Epidemiology and Infection 132 5 (2004): 997-999. http://www.scopus.com/inward/record.url?eid=2-s2.0-4744362250&partnerID=40&md5=400dd165f414807ee23cec81b0b296a3.
    10.1017/S0950268804212900

Other

Other output
  1. CD44v6 expression is a novel predictive marker of therapy response and poor prognosis in gastric cancer patients. 2018. Carla Pereira; Daniel Ferreira; Carolina Lemos; Diana Martins; Nuno Mendes; Daniela Almeida; Pedro Granja; et al. https://doi.org/10.1101/468934.
    10.1101/468934
Activities

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2022/11/22 - 2022/11/23 Formação realizada nos dias 22 e 23 de Novembro de 2022 “PhenoImager, Akoya Bioscience” , com a duração de 12 horas
Other
 Formação no “PhenoImager, Akoya Bioscience”
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022/11/01 - 2022/11/01 Participação em trabalho apresentado numa conferência online: Brás AR, Lopes N, Gartner F, Preto A, Valente A. New ruthenium-cyclopentadienyl complexes target specifically mutated KRAS in colorectal cancer. 8th International Electronic Conference on Medicinal Chemistry session Small molecules as drug candidates, Published: 01 November 2022
Other
 Participação em póster apresentado em conferência - 8th International Electronic Conference on Medicinal Chemistry
MDPI AG, Switzerland
2022/05/26 - 2022/05/27 Participação em póster apresentado em evento: Brás, Ana Rita; Lopes, Ana; Mendes, Nuno ; Fernandes, PedroA; Moreira, Tiago; Gärtner, Fátima; Valente, Andreia; Preto, Ana. New ruthenium-based complex for specific therapy of colorectal cancers with KRAS mutation. CQE days 2022, 26-27th of May , Lisbon
Other
 CQE days 2022
Universidade de Lisboa Faculdade de Ciências, Portugal
2017/01/01 - 2017/01/01 Participação em póster apresentado em evento: Palmeira C, Gaiteiro C, Mendes N, Gartnerf, Rocha L, Schaberle F, Martins G, Santos L. Redaporfin PDT combined with Anti-PD-1 treatment in melanoma mouse model: alterations in T-cell subsets. Reunião Anual da Sociedade Portuguesa de Imunologia, i3S ¿ Instituto de Investigação e Inovação em Sáude, Porto, Portugal, 2017
Other
 Participação em Póster apresentado numa conferência - Reunião Anual da Sociedade Portuguesa de Imunlogia
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2023/04/11 - 2023/04/14 The main aim of this course is to present the fundamentals of organoid models, specifically intestinal, gastric, and lung models. Participants will learn the basic principles of working with these models, and how to culture and maintain them. Moreover, they will be instructed on how to plan and run experiments using organoids, as well as on methods to analyze experimental data/ results. Microfluidics and organs-on-chip will also be explored. The specific protocols used for imaging, molecular biology, and histology using organoids will be covered. (Pós-Graduação) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022/11/21 - 2022/11/25 Theoretical and practical training on histology fundamentals when using 3D structures, namely from lung, gastric and intestinal organoids cultures. Advanced Course on Organoid Models - 2nd Edition (Pós-Graduação) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022/04/26 - 2022/04/29 Theoretical and practical training on histology fundamentals when using 3D structures, namely from lung, gastric and intestinal organoids cultures. Advanced Course on Organoid Models - 1st Edition Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2009/05/01 - 2009/12/31 Imunohistoquímica Anatomia Patológica, Citológica e Tanatológica (Licenciatura) Instituto Politécnico do Porto Escola Superior de Saúde, Portugal
2009/05/01 - 2009/12/31 Projeto em Anatomia Patológica Anatomia Patológica, Citológica e Tanatológica (Licenciatura) Instituto Politécnico do Porto Escola Superior de Saúde, Portugal

Scientific expedition

Activity description Institution / Organization
2006/12/01 - 2006/12/14 Visit to Pathology Department of Hospital Central de Maputo, Maputo, Mozambique, in order to conclude the formation of a Mozambican Laboratory Technician concerning basic molecular biology and histochemical procedures. This visit was included in the context of a research project between IPATIMUP and Hospital Central de Maputo. Hospital Central de Maputo, Mozambique