Manuel José Estevez Prieto (081E-5F1A-C476)

Curriculum Vitae

Manuel José Estevez Prieto

Manuel Prieto (PhD, 1981, Habilitation, 1993) is Full Professor of Instituto Superior Técnico (IST) of Universidade de Lisboa (Portugal), and Past-President of IUPAB (http://iupab.org/)(2024-2027). The work (February 2024) (ISI Web of Science: 264 publications , and 15 book chapters, 7242 citations; Google Scholar: 294 publications, 10144 citations ) obtained international recognition, in the area of advanced photophysical methodologies in life sciences, and object of invited communications, including a significant number of plenary talks. His work is centered in Molecular and Cell Biophysics, namely on the application of state-of-the art fluorescence methodologies, both in ensemble average and under the microscope, allowing to retrieve topological and dynamic information on biological systems, using quantitative approaches. Examples of the broad research interests, are membrane biophysics such as phase diagrams, detection and study of membrane nanodomains, lipid-protein/peptide and lipid-DNA interaction, amyloid fiber formation, and ion channels. At IST he has been involved in the teaching of Structural Biology, General Chemistry, and Spectroscopy, receiving the award of Excellent Teacher (2012, 2013). Of his previous PhD students (28 supervised/co-supervised), a significant number went into high profile careers such as Full Profs. (Univ. Lisboa), Assoc. Profs. (Univ. Coimbra, Porto, Lisboa and Madeira), Assistant Profs. (Univ. Lisboa and Coimbra), Assistant Res. (Univ. Lisboa, Nova), scientific edition (As. Editor, Nature; Senior Editor, Spandidos Pub.) and industry. MP is at the origin of the Portuguese biophysics school, is the founder of the Portuguese Biophysical Society, and has been deeply involved in Schools of Biophysics in Europe, Latin-America and Asia, both as organizer and lecturer, and is International Mentor of the Biophysical Society (USA). MP has a strong involvement in science outreach activities both in Portugal (secondary school presentations to >6000 students, Chemistry Olympiad, European Researchers Night), and abroad (Brazil, Uruguay). MP was the PI or co-PI of a large number of projects, both national (FCT), transnational (Brazil, Canada, Spain), and European (Marie-Curie, COST, EUREKA), and has also been actively involved in the organization of many international congresses. He is member of the Editorial Board of Biochim. Biophys. Acta and Chem. Phys. Lipids (Elsevier), Biophys. Rev. (Springer), Int. J. Mol. Sciences (MDPI), Membranes (MDPI), and Associate Editor of Membrane Physiology and Biophysics (Frontiers). MP has a regular participation as evaluator of international agencies e.g., NSF (USA), EPSRS (UK), ANR (France), NOSR (Netherlands), CNF (Czech Republic), CSIR (India). At the European level, he has been evaluator for Marie-Curie and COST actions. Also participated abroad in committees for academic positions, and Habilitation and PhD thesis. At the national level, he was member of the Scientific Council for Exact Sciences and Engineering (FCT, Portugal). MP has a strong record of participation in international scientific organizations: i) Council of LAFeBS (Latin American Fed. of Biophys. Societies), ii) Science Europe- Sci. Committee, iii) FEBS Publications Committee (2009-2013), iv) Collaborator Initiative for Science in Europe (2013). Also, he was Faculty Member of the UNESCO Chair of Biophysics (Argentina, 1999-2003), President of EBSA (European Biophysical Societies' Association), and is President of the major Biophysics organization, IUPAB (International Union of Pure and Applied Biophysics). He is also Emeritus Member of BPS. MP received the awards: 2005- Estímulo à Excelência - FCT (Portugal); 2006- Prémio União Latina, UTL (Portugal); 2007- Prémio Científico UTL/ Santander Totta- Biophysics Hon. Mention; 2008- Prémio Científico UTL/ Santander Totta- Biophysics; 2011- Honorary Member of the Spanish Biophysical Society; 2013-Distinguished Visitor-Univ.de Córdoba, Argentina.

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Identification

Personal identification

Full name: Manuel José Estevez Prieto
Date of birth: 1949/03/30
Gender: Male

Citation names

Prieto, Manuel
Prieto, MJ
Prieto, MJE

Author identifiers

Ciência ID: 081E-5F1A-C476
ORCID iD: 0000-0003-3012-6598

Email addresses

manuel.prieto@tecnico.ulisboa.pt (Professional)

Telephones

Telephone

(351) 2184193251 (Professional)

Addresses

iBB-Institute for Bioengineering and Biosciences, Complexo Interdisciplinar, IST, Av. Rovisco Pais, 1049-001, Lisboa, Lisboa, Portugal (Professional)

Websites

http://web.ist.utl.pt/ist10881/ (Professional)

Knowledge fields

Natural sciences - Biological Sciences - Biophysics

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Portuguese (Mother tongue)
English	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)
French	Advanced (C1)	Proficiency (C2)	Intermediate (B1)	Proficiency (C2)	Proficiency (C2)
Spanish; Castilian	Proficiency (C2)	Proficiency (C2)	Advanced (C1)	Proficiency (C2)	Proficiency (C2)
Italian	Elementary (A2)	Intermediate (B1)	Beginner (A1)	Intermediate (B1)	Intermediate (B1)
Galician	Upper intermediate (B2)	Proficiency (C2)	Intermediate (B1)	Proficiency (C2)	Proficiency (C2)

Education

	Degree	Classification
1993 Concluded	Engenharia Química (Título de Agregado) Universidade de Lisboa Instituto Superior Técnico, Portugal	Unanimidade
1982 Concluded	Engenharia Química (Doutoramento) Universidade de Lisboa Instituto Superior Técnico, Portugal	Cum Laude
1966 - 1971 Concluded	Engenharia Química (Licenciatura) Universidade de Lisboa Instituto Superior Técnico, Portugal	16 valores

Affiliation

Teaching in Higher Education

	Category Host institution	Employer
2011 - Current	Full Professor (University Teacher)	Universidade de Lisboa Instituto Superior Técnico, Portugal
	Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
1992/02/01 - 2011/05/30	Associate Professor (University Teacher)	Universidade de Lisboa Instituto Superior Técnico, Portugal
	Universidade de Lisboa Instituto Superior Técnico, Portugal
1982/01/02 - 1992/01/01	Assistant Professor (University Teacher)	Universidade de Lisboa Instituto Superior Técnico, Portugal
	Universidade de Lisboa Instituto Superior Técnico, Portugal
1971 - 1981	Assistant (University Teacher)	Universidade de Lisboa Instituto Superior Técnico, Portugal
	Universidade de Lisboa Instituto Superior Técnico, Portugal
1968 - 1970	Lecturer (University Teacher)	Universidade de Lisboa Instituto Superior Técnico, Portugal
	Universidade de Lisboa Instituto Superior Técnico, Portugal

Projects

Grant

	Designation	Funders
2017 - 2018	Membrane binding of retinol dehydrogenase 8 and its C-terminal segment Vison Research Network Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal	Natural Sciences and Engineering Research Council of Canada Concluded
2016/01/01 - 2016/12/31	Mechanism for the abolition of the enzymatic activity of the lecithin retinol acyltransferase mutants which lead to pigmentary retinitis and congenital Leber's amaurosis Réseau de recherche en santé de la vision, (RRSV), Québec, Canada Researcher	Natural Sciences and Engineering Research Council of Canada Concluded
2014 - 2015	Hybrid Polymer/Lipid Vesicles as New Particles for Drug-Delivery and Cell Mimics IDS-FunMat- ERASMUS MUNDUS Principal investigator Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	European Commission Seventh Framework Programme for Research and Technological Development Concluded
2008 - 2010	Clustering of GPI-anchored proteins in rafts model systems and in cell membranes. From membrane biophysiscs to cell biology Bilateral Coperation Portugal-India), FCT Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2005 - 2009	European Marie-Curie Action EST “BioMem" European Marie-Curie Action EST “BioMem" Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	European Commission Seventh Framework Programme for Research and Technological Development Concluded
2006 - 2007	Interaction of W-rich membrane peptides and proteins (HIV, HCV, SARS) with lipid rafts Acção Integrada Luso-Espanhola-E-41/06 Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	Concluded
2000 - 2005	European Concerted Research Action Cost D:22 (Protein-Lipid Interactions) European Concerted Research Action Cost D:22 (Protein-Lipid Interactions) Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	European Commission Seventh Framework Programme for Research and Technological Development Concluded
2000 - 2001	Time-resolved fluorescence of channel-inhibiting and non-inhibiting peptides dependent on a potential difference Acção Integrada Luso-Espanhola (E-10/00) Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal	Concluded
1999 - 2000	Structure and interaction with model membranes of two peptides derived from the human immunodeficiency virus (HIV-1) protein gp41 Acção Integrada Luso- Espanhola. (E-41/99) Acção Integrada Luso - Espanhola 1997. (E-36/97) Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	Concluded
1992 - 1995	Biophysical applications of spectroscopy with nano and picosecond temporal resolution Acção Integrada Luso - Espanhola Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	Concluded
1990 - 1992	Modification of model membranes and biomembranes by incorporation of diacylglycerols Acção Integrada Luso - Espanhola Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	Concluded
1987 - 1989	Gradient of water concentration in biomembranes Acção Integrada Luso - Espanhola Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	Concluded

Contract

	Designation	Funders
2021/01/01 - 2025/12/31	Institute for Health and Bioeconomy LA/P/0140/2020 10.54499/LA/P/0140/2020 Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal Universidade Nova de Lisboa, Portugal Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal Unidade de Ciências Biomoleculares Aplicadas, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal INESC Microsistemas e Nanotecnologias, Portugal Universidade Nova de Lisboa Associação para a Inovação e Desenvolvimento da FCT, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2019/01/01 - 2023/12/31	Applied Molecular Biosciences Unit UIDP/04378/2020 10.54499/UIDP/04378/2020 UIDB/04378/2020 10.54499/UIDB/04378/2020 Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal Universidade Nova de Lisboa, Portugal Universidade do Porto, Portugal Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal Unidade de Ciências Biomoleculares Aplicadas, Portugal Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal INESC Microsistemas e Nanotecnologias, Portugal Universidade Nova de Lisboa Associação para a Inovação e Desenvolvimento da FCT, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2022/01/17 - 2023/07/16	Engineering broad-spectrum defensosomes for a bacterial antivirulence therapeutic strategy EXPL/BTM-MAT/0910/2021 Researcher Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2018/10/01 - 2021/09/30	Exploring the biophysical link between Gaucher disease and Parkinson's disease PTDC/BIA-BFS/29448/2017 Researcher Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal Universidade de Lisboa Faculdade de Farmácia, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2018/10/01 - 2021/09/30	Organoid Engineering for Production of 3D Cardiovascular Microtissues from Human Induced Pluripotent Stem Cells for Cardiotoxicity Assays PTDC/EMD-TLM/29728/2017 Researcher Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2018/10/01 - 2021/09/30	The aggregation-prone structures of Huntingtin exon 1 at the single-molecule level: Influence of membranes and implications in Huntington disease PTDC/BIA-BFS/30959/2017 Researcher Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2018/08/10 - 2021/08/09	Engenharia de Exossomas Inteligentes para Remoção de Proteína beta-amilóide na doença de Alzheimer. PTDC/BTM-SAL/31057/2017 Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2020/01/06 - 2021	Bioactive lipids at the ocular surface: from Langmuir surface balance to ophthalmic nanoemulsions - EU Project info:eu-repo/grantAgreement/EC/H2020/839315/EU Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	European Commission Ongoing
2018 - 2021	Engineering of Smart Exosomes for Amyloid-beta Clearance in Alzheimer disease. PTDC/BTM-SAL/31057/2017 Researcher Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2017/01/01 - 2020/12/31	PRECISION ONCOLOGY BY INNOVATIVE THERAPIES AND TECHNOLOGIES SAICTPAC/0019/2015 Researcher Universidade de Lisboa Faculdade de Farmácia, Portugal Instituto de Engenharia de Sistemas e Computadores Microsistemas e Nanotecnologias, Portugal Instituto Politécnico de Leiria, Portugal Universidade de Coimbra, Portugal Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2017/01/15 - 2020/01/14	Lipid Oxidation in membrane and cell Biophysics: From Functional nanosensors to the impact on amyloid formation. Application of advanced fluorescence, X-Ray scattering and microscopy Techniques FAPESP/20107/2014 Principal investigator Universidade de Lisboa Centro de Química-Física Molecular, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2019/01/01 - 2019/12/31	Instituto de Nanociência e Nanotecnologia UID/NAN/50024/2019 INESC Microsistemas e Nanotecnologias, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2016/05/02 - 2019/12/31	Biophysical and biological properties of atypical sphingolipids: implications to physiology and pathophysiology PTDC/BBB-BQB/3710/2014 PTDC/BBB-BQB/3710/2014 Researcher Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal Universidade de Lisboa Faculdade de Farmácia, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2013/06/01 - 2016/11/30	MULTIFUNCTIONAL NANOMATERIALS FOR FLUORESCENCE IMAGING AND SENSING RECI/CTM-POL/0342/2012 Researcher Universidade de Lisboa Centro de Química-Física Molecular, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2013 - 2016	Ceramide platforms: the role of the membrane biophysical properties on the biological effects of ceramide PTDC/BBB-BQB/0506/2012 Researcher Universidade de Lisboa Faculdade de Farmácia, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2013/07/01 - 2015/12/31	Modulation of Calcitonin Amyloid Formation by Lipid Membranes and Extracellular Matrix: Implications for the Mechanism of Cytotoxicity of Amyloidogenic Polypeptides PTDC/BBB-BQB/2661/2012 Principal investigator Universidade de Lisboa Centro de Química-Física Molecular, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal Universidade de Coimbra, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2012/03/01 - 2015/08/31	Interaction of bile acids with lipid membranes: creating a shield or bursting the bubble. PTDC/QUI-BIQ/119494/2010 info:eu-repo/grantAgreement/FCT/3599-PPCDT/119494/PT Researcher Universidade de Lisboa Centro de Química-Física Molecular, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal Universidade de Lisboa Faculdade de Farmácia, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2011/02/01 - 2014/07/31	Relationship between PI(4,5)P2 compartmentalization and palmitoylated synaptic proteins in membrane model systems and in the plasma membrane of neuron and non-neuron like cells. PTDC/QUI-BIQ/112067/2009 Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal Universidade de Lisboa Centro de Química-Física Molecular, Portugal Universidade de Évora, Portugal Instituto de Medicina Molecular, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2011/01/01 - 2014/06/30	Ceramide and Glucosylceramide impact on membrane biophysical properties: from models of plasma- and lysosomal-membranes to the cell membrane PTDC/QUI-BIQ/111411/2009 info:eu-repo/grantAgreement/FCT/3599-PPCDT/111411/PT Researcher Universidade de Lisboa Faculdade de Farmácia, Portugal Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010/01/01 - 2013/06/30	Amyloidogenic proteins: role of lipid-protein interaction in amyloid-like fibril formation PTDC/QUI-BIQ/099947/2008 info:eu-repo/grantAgreement/FCT/3599-PPCDT/99947/PT Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal Universidade de Lisboa Centro de Química-Física Molecular, Portugal Universidade de Évora, Portugal Instituto de Medicina Molecular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010/01/01 - 2013/06/30	Bacterial protein azurin as a new candidate drug to treat poor-prognosis breast cancer PTDC/EBB-BIO/100326/2008 info:eu-repo/grantAgreement/FCT/3599-PPCDT/100326/PT Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal Instituto de Biotecnologia e Bioengenharia, Portugal Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2011/01/01 - 2012/12/31	Strategic Project - LA 24 - 2011-2012 PEst-OE/CTM/LA0024/2011 Universidade do Porto, Portugal Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal INESC Microsistemas e Nanotecnologias, Portugal Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2009 - 2011	Behaviour of fluorescent membrane probes in lipid bilayers: combining theory and experiment PTDC/QUI-QUI/098198/2008 Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2007/10/01 - 2010/09/30	Lipid Rafts, Ceramide, and Human Immunodeficiency Virus: a Biophysical Connection info:eu-repo/grantAgreement/FCT/3599-PPCDT/68151/PT Researcher	Fundação para a Ciência e a Tecnologia Concluded
2005/09/01 - 2008/06/30	Molecular interactions in lipid raft models: from lipid phases to membrane protein organization POCI/QUI/57123/2004 POCTI/QUI/57123/2004 Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal Universidade de Lisboa Centro de Química-Física Molecular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2005/06/01 - 2008/05/31	Molecular mechanism of inhibition of the vacuolar H+-ATPase of osteoclasts by potential antiosteporotic drugs POCI/SAU-FCF/57114/2004 POCI/SAU-FCF/57114/2004 Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal Universidade de Lisboa Centro de Química-Física Molecular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2000/09/01 - 2004/09/01	The Effect of Membrane Microdomains on the Mode of Action of Polyene Antibiotics at the Molecular Level. The Role of Sterols info:eu-repo/grantAgreement/FCT/POCI/36389/PT Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2002 - 2004	Ultra-fast molecular and supramolecular dynamics of excited states and microscopy: Applications in chemistry, biochemistry, biophysics and materials sciences CON-REEQ/487/2001 Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia
2001/05/01 - 2003/11/01	Phase behaviour of biological membrane components: kinetics, thermodynamics, composition and topology. A photophysical study. info:eu-repo/grantAgreement/FCT/POCI/36458/PT Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2000/11/01 - 2002	The interaction of HIV-1 membrane protein gp41 fusion peptide with biomembranes and its inhibition JNICT/PRAXIS/SAU/14025/1998 PRAXIS/P/BIO/12049/1998 Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
1997 - 1999	Structural study of the interaction of the hemagglutinin protein from the influenza virus (Influenza) and a peptide derived from the gp41 protein (HIV-1), with model membrane systems JNICT, PRAXIS/PCNA/P/BIO/56/96 Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
1996 - 1998	Action of weak acid preservatives in yeast saccharomyces cerevisiae: acquisition of tolerance and underlying mechanisms Programa Base de Investigação Científica e Tecnológica (JNICT 1996-1998) Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
1996 - 1997	Modeling the mode of action of fungicidal polyenic antibiotics on membranes. The relevance of sterols PECS/C/SAU/144/95 Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
1993 - 1997	Use of supramolecular biovectors for the transport of immunoactive molecules EUREKA (PUEM/S/ERC/53/93) Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	European Commission Seventh Framework Programme for Research and Technological Development Concluded
1993 - 1994	The physiological relevance of cholesterol and ergosterol in the mechanism of action of fungicidal antibiotics STRD/SAU/257/92 Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
1991 - 1992	Photophysical processes in homogeneous and heterogeneous media CIÊNCIA. (0014/M/90) Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
1987 - 1989	National Network of Photophysics and Photochemistry Subprograma III, Medida M Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Other

	Designation	Funders
1996 - 1998	Molecular probe spectroscopy Centro de Química-Física Molecular (Linha I) Principal investigator Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Outputs

Publications

Book chapter	Loura, Luís M. S.; Prieto, Manuel; Fernandes, Fábio. "Förster Resonance Energy Transfer as a Tool for Quantification of Protein–Lipid Selectivity". In Methods in Molecular Biology, 369-382. Springer New York, 2019. Published • 10.1007/978-1-4939-9512-7_16 Sousa, Tânia; Castro, Rui E.; Coutinho, Ana; Rodrigues, Cecília M. P.; Prieto, Manuel; Fernandes, Fábio. "Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy". In Experimental Cholestasis Research, 99-115. Springer New York, 2019. Published • 10.1007/978-1-4939-9420-5_7 Dao, Thi Phuong Tuyen; Ferji, Khalid; Fernandes, Fabio; Prieto, Manuel; Lecommandoux, Sébastien; Ibarboure, Emmanuel; Sandre, Olivier; Meins, Jean-François Le. "Giant hybrid polymer/lipid vesicles". In The Giant Vesicle Book, 551-568. CRC Press, 2019. Published • 10.1201/9781315152516-27 Loura, Luís M. S.; Prieto, Manuel. "Fluorescence and FRET in Membranes". In Encyclopedia of Biophysics, 1-6. Springer Berlin Heidelberg, 2019. Published • 10.1007/978-3-642-35943-9_559-1 Prieto, Manuel; Berberan-Santos, Mário N.. "Molecules full of energy: Light and colors in nature". In Enseñanza de Ciencias Básicas, edited by Domínguez L., Peluffo R. D. Uruguay: CENUR Litoral Norte. Universidad de la Republica Uruguaya, 2018. Published Prieto, Manuel; F. Fernandes; L. Loura. "Biophysical studies of V-ATPase inhibitors in model membranes". In A Toxicological/Pharmacological Approach to Chemico-Biological Interactions at a Membrane Level, edited by A. Jurado. R. Signpost, 2013. Published Prieto, Manuel. "Fluorescence and FRET". In Encyclopedia of Biophysics. Springer, 2013. Published Loura, Luís M. S.; Prieto, Manuel. "Lateral Membrane Heterogeneity Probed by FRET Spectroscopy and Microscopy". In Fluorescent Methods to Study Biological Membranes, 71-113. Springer Berlin Heidelberg, 2012. Published • 10.1007/4243_2012_59 Prieto, Manuel; L. Loura. "Time-resolved fluorescence methodologies in the study of lipid-peptide interaction". In Membrane-Active Peptides: Methods and Results on Structure and Function, edited by M. Castanho. International University Line, 2010. Published Loura, Luís M.S.; Prieto, Manuel. "Resonance Energy Transfer in Biophysics: Formalisms and Application to Membrane Model Systems". In Fluorescence of Supermolecules, Polymers, and Nanosystems, edited by M. N. Berberan-Santos, 299-322. Springer Berlin Heidelberg, 2007. Published • 10.1007/4243_2007_016 Mateo, C. Reyes; de Almeida, Rodrigo F.M.; Loura, Luis M.S.; Prieto, Manuel. "From Lipid Phases to Membrane Protein Organization: Fluorescence Methodologies in the Study of Lipid-Protein Interactions". In Springer Series in Biophysics, 1-33. Springer Berlin Heidelberg, 2006. Published • 10.1007/3-540-28435-4_1 Prieto, Manuel; R. F. M. de Almeida; J. W. Borst; M. Hink; T. Visser. "Lipid domains and rafts studied by time-resolved fluorescence microspectroscopy". In Biochemistry and biophysics of lipids, edited by A. Pramanik, 31-62. Research Signpost, 2006. Published Antollini, S; Baier, J; Blanton, M; Bonini, I; De los Santos, B; Gallegos, M; Garbus, I; et al. "Structure and dynamics of acetylcholine receptor and its lipid microenvironment". In Cholinergic Mechanisms, 33-38. CRC Press, 2004. Published • 10.3109/9780203493878-6 Castanho, Miguel A. R. B.; Prieto, Manuel J. E.; Betbeder, Didier; Santos, Nuno C.. "The Shape, Dimension and Organisation of Maltodextrins Gel Fragments with and without Associated Phospholipids". In Light Scattering and Photon Correlation Spectroscopy, 173-187. Springer Netherlands, 1997. Published • 10.1007/978-94-011-5586-1_15
Conference abstract	Varela, A. R.; Futerman, A. H.; Prieto, M.; Silva, L. C.. "Biomembrane properties in gaucher disease: (Glyco) sphingolipid impact". 2013. Mello-Vieira, Joao; Coutinho, Ana; Fedorov, Alexander; Lucas, Sandra D.; Moreira, Rui; Castro, Rui E.; Rodrigues, Cecilia; Prieto, Manuel; Fernandes, Fabio. "The Apoptotic Bile Acid DCA has Preference for Association to Liquid Disordered Lipid Domains and Inhibits the Rigidifying Effect of Cholesterol in Membranes". 2013. 10.1016/J.BPJ.2012.11.3261 Sarmento, Maria Joao; Coutinho, Ana; Fedorov, Aleksander; Prieto, Manuel; Fernandes, Fabio. "Physiological Calcium Concentrations Induce PI(4,5)P2 Clustering: PI(4,5)P2 as a Lipidic Calcium Sensor". 2013. 10.1016/J.BPJ.2012.11.2069 Fernandes, F.; Sarmento, M. J.; Prieto, M.. "Tethering of lipid membranes reorganizes the distribution of lipid domains". 2012. Sarmento, Maria J.; Prieto, Manuel; Fernandes, Fabio. "High Affinity Immobilization of Giant Unilamellar Vesicles (GUVs) Induces Redistribution of Lipid Domains". 2012. 10.1016/J.BPJ.2011.11.1634 Melo, Ana M.; Fedorov, Aleksander; Prieto, Manuel; Coutinho, Ana. "Exploring Fluorescence Lifetime and Homo-FRET Measurements to Monitor Lysozyme Oligomerization in Anionic Lipid Membranes: Relation to "Amyloid-Like" Fibril Formation". 2012. 10.1016/J.BPJ.2011.11.2375 Mello-Vieira, J.; Fedorov, A.; Coutinho, A.; Lucas, S. D.; Moreira, R.; Castro, R. E.; Rodrigues, C. M. P.; Prieto, M.; Fernandes, F.. "Lipid membranes as possible targets for apoptotic bile acids". 2012. Melo, A. M.; Fedorov, A.; Prieto, M.; Coutinho, A.. "'Amyloid-like' fibril formation driven by anionic lipid membranes: multiparametric fluorescence detection of lysozyme oligomeric intermediates". 2012. Castro, B. M.; Mollinedo, F.; Hornillos, V.; Guerri, F. A.; Acuna, A. U.; Prieto, M.. "Edelfosine and miltefosine fluidize lipid rafts: implications for Fas-mediated apoptosis triggering". 2012. Pinto, Sandra N.; Silva, Liana C.; Futerman, Anthony H.; Prieto, Manuel. "The role of ceramide acyl chain length and unsaturation on membrane structure". 2011. Castro, Bruno M.; de Almeida, Rodrigo F. M.; Prieto, Manuel. "Membrane organization of Fas transmembrane domain: Insights for clustering and apoptosis triggering". 2011. Sarmento, Maria J.; Pinto, Sandra N.; Fernandes, Fabio; Prieto, Manuel. "Regulation of PIP2 clustering in neuron-like model cells by synaptic protein palmitoylation". 2011. Melo, Ana M.; Coutinho, Ana; Prieto, Manuel. "Lipid-protein interaction and amyloid-like fiber formation: FLIM and FCS studies". 2011. Sarmento, Maria Joao; Prieto, Manuel; Fernandes, Fabio. "Influence of avidin-coated surface for GUV immobilization on lipid domain size and distribution". 2011. Pinto, Sandra N.; Silva, Liana C.; de Almeida, Rodrigo F. M.; Prieto, Manuel. "Ceramide Gel Domain Formation in a Phospholipid Bilayer: The Impact of Ceramide Acyl Chain". 2010. 10.1016/J.BPJ.2009.12.4251 Coutinho, A.; Melo, A.; Loura, L.; Prieto, M.. "Amyloid-like fiber formation: the role of lipid-protein interaction". 2010. Castro, Bruno M.; Silva, Liana C.; de Almeida, Rodrigo F. M.; Prieto, Manuel. "Cholesterol-Rich Fluid Membranes Solubilize Ceramide Gel Domains. Implications for the Organization of Mammalian Membranes". 2010. 10.1016/J.BPJ.2009.12.1248 Silva, Liana C.; Futerman, Anthony H.; Prieto, Manuel. "Lipid Raft Composition Modulates Sphingomyelinase Activity and Ceramide-Induced Membrane Physical Alterations". 2010. 10.1016/J.BPJ.2009.12.1094 Bastos, Margarida; Adao, Regina; Nazmi, Kamran; Uhrikova, Daniela; Funari, Sergio S.; Coutinho, Ana; Prieto, Manuel; Bolscher, Jan. "LFampin Derived Antimicrobial Peptide: Biophysical Characterization and Biological Implications of Composition and Structure". 2010. 10.1016/J.BPJ.2009.12.476 Castro, Bruno; Pinto, Sandra; Silva, Liana; Prieto, Manuel. "Rafts and ceramides: biophysical perspective of biologically relevant membrane domains". 2010. 10.1016/j.chemphyslip.2010.05.008 Silva, Liana; Castro, Bruno; de Almeida, Rodrigo; Pinto, Sandra; Prieto, Manuel. "Biophysics of rafts and their interplay with ceramides". 2008. 10.1016/j.chemphyslip.2008.05.005 Cabre, Elisa J.; Lourab, Luis M. S.; Olmeda, Barbara; Fedorov, Alexander; Perez-Gil, Jesus; Prieto, Manuel. "Structural characterization of pulmonary surfactant protein SP-B in model membranes by fluorescence spectroscopy". 2007. 10.1016/j.chemphyslip.2007.06.027 Silva, Liana C.; de Almeida, Rodrigo F. M.; Castro, Bruno M.; Prieto, Manuel. "Ceramide recruits 3 sphingomyelin molecules to form nanometer-sized gel domains that are destroyed by cholesterol in lipid rafts". 2007. Pinto, Sandra; Silva, Liana; Almeida, Rodrigo; Fedorov, Alexander; Prieto, Manuel. "Biophysical behaviour of N-nervonoyl-D-erythro-sphingosine and its interaction with a fluid phospholipid". 2007. 10.1016/j.chemphyslip.2007.06.088 Silva, Liana C; DE ALMEIDA, RODRIGO F.M.; Castro, Bruno M.; Prieto, Manuel. "Modulation of lateral membrane organization by the interplay of ceramide with lipid rafts". Paper presented in 6th EBSA & British Biophysical Society Congress July 14, London, 2007. Published De Almeida, R. F. M.; Loura, L. M. S.; Prieto, M.; Barrantes, F. J.. "Neural glycolipids and glycoproteins in interaction with raft containing membranes". 2006. Silva, L. C.; De Almeida, R. F. M.; Castro, B. M.; Prieto, M.. "Ceramide-domain formation and collapse in lipid rafts: membrane reorganization by an apoptotic lipid. Comparison to ganglioside GM(1)". 2006. Bai, G.; Gomes, P.; Seixas, R.; Hicks, M.; Prieto, M.; Bastos, M.. "Insights on the interactions between two cecropin-melittin peptides and model membranes: Calorimetric and spectroscopic results". 2006. Mano, M.; Henriques, A.; Paiva, A.; Prieto, M.; Gavilanes, F.; Simoes, S.; de Lima, M. C. Pedroso. "Cellular uptake of S4(13)-PV cell penetrating peptide: An endocytosis-independent process following peptide conformational changes induced by peptide-membrane interactions". 2006. Silva, Liana C; DE ALMEIDA, RODRIGO F.M.; Federov, Alexander; Prieto, Manuel. "Ceramide domain formation and its role on sphingomyelin/cholesterol raft modulation". Paper presented in 50th Annual Meeting Biophysical Society, Salt Lake City, Utah, 2006. Published Silva, Liana C; DE ALMEIDA, RODRIGO F.M.; Federov, Alexander; Prieto, Manuel. "Ceramide in low concentrations forms large and rigid platforms in fluid membranes". Paper presented in 50th Annual Meeting Biophysical Society, Salt Lake City, Utah, 2006. Published • 10.4271/820843 Fernandes, F; Loura, L; Koehorst, R; Hemminga, M; Prieto, M. "Interaction of the indole class of V-ATPase inhibitors with lipid bilayers.". 2005. de Almeida, RFM; Loura, LMS; Fedorov, A; Prieto, M. "Lipid rafts can have different sizes depending on membrane composition: A time-resolved fluorescence resonance energy transfer study". 2005. 10.1016/j.jmb.2004.12.026 Silva, L; de Almeida, RFM; Fedorov, A; Prieto, M. "Ceramide affects the properties of sphingomyelin-cholesterol based lipid rafts". 2005. Silva, L; de Almeida, RF; Fedorov, A; Prieto, M. "Biophysical properties of a lipid bilayer containing ceramide: relevance to the biochemical role of ceramide". 2005. DE ALMEIDA, RODRIGO F.M.; Borst, J. W.; Visser, A.J.W.G.; Prieto, Manuel. "Fluorescence lifetime imaging microscopy (FLIM) of membrane lipid domains and rafts". Paper presented in 5th EBSA & 15th IUPAB International Biophysics Congress, Montpellier, 2005. Published DE ALMEIDA, RODRIGO F.M.; Loura, Luís M. S.; Federov, Alexander; Prieto, Manuel. "Rafts big and small. Their size determination on a ternary membrane phase diagram". Paper presented in 5th EBSA & 15th IUPAB International Biophysics Congress, Montpellier, 2005. Published Madeira, C; Loura, L; Aires-Barros, R; Fedorov, A; Prieto, M. "Quantification of free DNA in lipid-DNA complexes using FRET". 2004. de Almeida, RFM; Prieto, M. "A model for raft/non-raft coexistence. Phosphatidylcholine/sphingomyelin/cholesterol ternary phase diagram.". 2003. Coutinho, A; Silva, L; Fedorov, A; Prieto, M. "Probing the role of sterols in the mechanism of aggregate formation by nystatin in membranes. A fluorescence study". 2003. Barrantes, FJ; Antollini, SS; Blanton, MP; Prieto, M. "Two architectural designs in nicotinic acetylcholine receptor membrane-embedded domains". 2001. Lopes, SDDN; Fernandes, M; Castanho, M; Prieto, M. "Orientational order of the membrane probe polyene fatty acid, trans-Parinaric Acid, in LB multilayer films". 2001. de Almeida, RFM; Loura, LMS; Barrantes, FJ; Prieto, M. "Interaction of the Gamma M4 transmembrane segment of the acetylcholine receptor with cholesterol-rich phases.". 2001. Santos, NC; Ter-Ovanesyan, E; Zasadzinski, JA; Prieto, M; Castanho, MARB. "AFM imaging of filipin induced lesions on planar phospholipid bilayer membranes". 1998. Castanho, MARB; Jameson, DM; Prieto, M. "Filipin incorporated in DPPC bilayers shows preference for more rigid media. A pressure dependence study". 1998. Coutinho, A; Prieto, M. "Interaction between nystatin and phospholipid vesicles: The sterol dependence.". 1996. CASTANHO, MARB; BROWN, W; PRIETO, MJE. "STATIC AND DYNAMIC LIGHT-SCATTERING-STUDIES OF FILIPIN IN AQUEOUS-MEDIA AND ITS INTERACTION WITH CHOLESTEROL". 1993.
Conference poster	García, Clara; Renart, María Lourdes; Giudici, Ana Marcela; Poveda, José Antonio; González-Ros, José M.; Berberan-Santos, Mário N.; Prieto, Manuel; Coutinho, Ana. "Molecular basis of ion channel modulation: Mapping the conformational states of the outer vestibule of KcsA, a prokaryote potassium channel, using homo-FRET.". Paper presented in Joint 12th EBSA-10th ICBP-IUPAP Biophysics Congress. Madrid, Spain., 2019. Borges-Araújo, L.; Monteiro, M.E.; Mil-Homens, D.; Bernardes, N.; Sarmento, Maria J.; Coutinho, A.; Prieto, Manuel; Melo, M.N.; Fernandes, F.. "Impact of Ca2+-dependent PI(4,5)P2 clustering on dynamics of PI(4,5)P2 binding proteins.". Paper presented in Joint 12th EBSA, 10th ICBP-IUPAP Biophysics Congres, 2019. Borges-Araújo, L.; Monteiro, M.E.; Mil-Homens, D.; Bernardes, N.; Sarmento, Maria J.; Coutinho, A.; Prieto, Manuel; Fernandes, F.. "Impact of Ca2+-dependent PI(4,5)P2 clustering on the properties of PI(4,5)P2 binding proteins.". Paper presented in 6th International Iberian Biophysics Congress ¿ X Iberoamerican congress of Biophysics, 2018. Díaz-García, Clara; Renart, María Lourdes; Poveda, José Antonio; Prieto, Manuel; Coutinho, Ana. "Structural insights into the molecular basis of lipid-dependent gating of KcsA, a model potassium channel.". Paper presented in XLII Congress of the Brazilian Biophysical Society (SBBf), São Paulo, Brazil., 2017. 10.1109/bmei.2011.6098715 Magalhães, Salomé; Duarte, Sofia; Monteiro, Gabriel A.; Prieto, Manuel; Fernandes, Fábio. "Trafficking Studies of plasmid DNA through Förster Resonance Energy Transfer". Paper presented in MicroBiotec’13 Congress of Microbiology and Biotechnology 2013, 2013. Magalhães, Salomé; Duarte, Sofia; Monteiro, Gabriel A.; Prieto, Manuel; Fernandes, Fábio. "Plasmid DNA delivery followed through Förster Resonance Energy Transfer". Paper presented in 9th EBSA European Biophysics Congress, 2013.
Journal article	Morán-Lalangui, Mishelle; Coutinho, Ana; Prieto, Manuel; Fedorov, Alexander; Pérez-Gil, Jesús; Loura, Luís M. S.; García-Álvarez, Begoña. "Exploring protein¿protein interactions and oligomerization state of pulmonary surfactant protein C (SP-C) through FRET and fluorescence self-quenching". Protein Science 33 1 (2023): http://dx.doi.org/10.1002/pro.4835. 10.1002/pro.4835 Ventura, Ana E.; Pokorna, Sarka; Huhn, Natalie; Santos, Tania C. B.; Prieto, Manuel; Futerman, Anthony H.; Silva, Liana C.. "Cell lipid droplet heterogeneity and altered biophysical properties induced by cell stress and metabolic imbalance". Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids (2023): https://publons.com/wos-op/publon/63015056/. 10.1016/J.BBALIP.2023.159347 Prieto, Manuel; Fernandes, Fabio; Tuyen Dao, T.. "Phase separation and dynamics on "hybrid polymer/lipid vesicles", from time-resolved fluorescence and microscopy". European Biophysics Journal (2023): https://publons.com/wos-op/publon/63686985/. Melo, Ana M.; Sousa, Tania; Damas, Goncalo; Coutinho, Ana; Bernardes, Nuno; Azevedo, Ana; Prieto, Manuel. "Probing the distinct conformational signatures of the flanking polyQ regions in huntingtin exon 1 at the membrane interface: insights into Huntington's disease". European Biophysics Journal (2023): https://publons.com/wos-op/publon/62961254/. Moran-Lalangui, Mishelle; Coutinho, Ana; Prieto, Manuel; Perez-Gil, Jesus; Loura, Luis M. S.; Garcia-Alvarez, Begona. "FRET studies of the Pulmonary Surfactant protein C (SP-C): oligomer stoichiometry and protein-protein interactions". European Biophysics Journal (2023): https://publons.com/wos-op/publon/63688396/. Coutinho, Ana; Diaz-Garcia, Clara; Renart, M. Lourdes; Giudici, Ana M.; Poveda, Jose A.; Gonzalez-Ros, Jose M.; Berberan-Santos, M. N.; Prieto, Manuel. "Probing the gating cycle of KcsA potassium channel using advanced homo-FRET approaches". European Biophysics Journal (2023): https://publons.com/wos-op/publon/64266619/. Fernandes, Fabio; Dao, T. Tuyen; Prieto, Manuel. "Hybrid polymer/lipid vesicles: Insights on phase separation and dynamics from time-resolved fluorescence and microscopy". Biophysical Journal (2023): https://publons.com/wos-op/publon/63686984/. Martinho, Nuno; Marques, Joaquim M. T.; Todoriko, Iryna; Prieto, Manuel; de Almeida, Rodrigo F. M.; Silva, Liana C.. "Effect of Cisplatin and Its Cationic Analogues in the Phase Behavior and Permeability of Model Lipid Bilayers". Molecular Pharmaceutics (2023): https://publons.com/wos-op/publon/56271760/. 10.1021/ACS.MOLPHARMACEUT.2C00321 Tiago P. Dias; Sandra N. Pinto; Sandra Carvalho; Tiago G. Fernandes; Fábio Fernandes; Maria Margarida Diogo; Maria C. Peleteiro; Manuel Prieto; Joaquim M. S. Cabral. "Cost-Effective Mechanical Aggregation of Cardiac Progenitors and Encapsulation in Matrigel Support Self-Organization in a Dynamic Culture Environment". International Journal of Molecular Sciences (2022): https://doi.org/10.3390/ijms232415785. 10.3390/ijms232415785 Tiago P. Dias; Tânia Baltazar; Sandra N. Pinto; Tiago G. Fernandes; Fábio Fernandes; Maria Margarida Diogo; Manuel Prieto; Joaquim M. S. Cabral; Ren-Ke Li. "Xeno-Free Integrated Platform for Robust Production of Cardiomyocyte Sheets from hiPSCs". Stem Cells International (2022): https://doi.org/10.1155/2022/4542719. 10.1155/2022/4542719 Luís Borges-Araújo; Marina E. Monteiro; Dalila Mil-Homens; Nuno Bernardes; Maria J. Sarmento; Ana Coutinho; Manuel Prieto; Fábio Fernandes. "Impact of Ca2+-Induced PI(4,5)P2 Clusters on PH-YFP Organization and Protein-Protein Interactions". Biomolecules (2022): https://doi.org/10.3390/biom12070912. 10.3390/biom12070912 Pokorna, Sarka; Ventura, Ana E.; Santos, Tânia C.B.; Hof, Martin; Prieto, Manuel; Futerman, Anthony H.; Silva, Liana C.. "Laurdan in live cell imaging: Effect of acquisition settings, cell culture conditions and data analysis on generalized polarization measurements". Journal of Photochemistry and Photobiology B: Biology 228 (2022): 112404. http://dx.doi.org/10.1016/j.jphotobiol.2022.112404. Published • 10.1016/j.jphotobiol.2022.112404 Prieto, Manuel; Gómez-Fernández, Juan Carmelo. "Foreword". Biochimica et Biophysica Acta (BBA) - Biomembranes 1864 2 (2022): 183827. http://dx.doi.org/10.1016/j.bbamem.2021.183827. Published • 10.1016/j.bbamem.2021.183827 • Editor Pokorna, Sarka; Ventura, Ana E.; Santos, Tania C. B.; Hof, Martin; Prieto, Manuel; Futerman, Anthony H.; Silva, Liana C.. "Laurdan in live cell imaging: Effect of acquisition settings, cell culture conditions and data analysis on generalized polarization measurements". Journal of Photochemistry and Photobiology B: Biology (2022): https://publons.com/wos-op/publon/54926986/. 10.1016/J.JPHOTOBIOL.2022.112404 Sousa, Tania; Bernardes, Nuno; Coutinho, Ana; Prieto, Manuel; Melo, Ana M.. "The conformational dynamics of the flanking polyQ regions in the membrane-bound state of huntingtin exon 1". Biophysical Journal (2022): https://publons.com/wos-op/publon/53621882/. Prieto, Manuel; Gomez-Fernandez, Juan Carmelo. "Foreword". Biochimica et Biophysica Acta (BBA) - Biomembranes (2022): https://publons.com/wos-op/publon/64266625/. 10.1016/J.BBAMEM.2021.183827 Sousa, T.; Damas, G.; Bernardes, N.; Coutinho, A.; Prieto, M.; Melo, A. M.. "The flanking polyQ regions of Huntingtin exon 1 display distinct dynamic signatures in its membrane-bound state: insights into Huntington's disease". FEBS Open Bio (2022): https://publons.com/wos-op/publon/58171357/. Diaz-Garcia, C.; Renart, M. L.; Giudici, A. M.; Poveda, J. A.; Fedorov, A.; Gonzalez-Ros, J. M.; Prieto, M.; Coutinho, A.. "Revealing the allosteric coupling during the gating cycle of the prototypical KcsA potassium channel using advanced homo-FRET approaches". FEBS Open Bio (2022): https://publons.com/wos-op/publon/64266626/. Prieto, Manuel. "On 'Fluorescence and the structure of proteins. I. Effects of substituents on the fluorescence of indole and phenol compounds' by Robert W. Cowgill". Archives of Biochemistry and Biophysics (2022): https://publons.com/wos-op/publon/64370308/. 10.1016/J.ABB.2022.109270 Díaz-García, Clara; Renart, Maria Lourdes; Poveda, José Antonio; Giudici, Ana Marcela; González-Ros, José M.; Prieto, Manuel; Coutinho, Ana. "Probing the Structural Dynamics of the Activation Gate of KcsA Using Homo-FRET Measurements". International Journal of Molecular Sciences 22 21 (2021): 11954. http://dx.doi.org/10.3390/ijms222111954. Published • 10.3390/ijms222111954 Sarmento, Maria J.; Borges-Araújo, Luís; Pinto, Sandra N.; Bernardes, Nuno; Ricardo, Joana C.; Coutinho, Ana; Prieto, Manuel; Fernandes, Fábio. "Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)P2 Confinement". International Journal of Molecular Sciences 22 21 (2021): 11727. http://dx.doi.org/10.3390/ijms222111727. Published • 10.3390/ijms222111727 Hädicke, André; Coutinho, Ana; Roy, Sarah; Otis, François; Lhor, Mustapha; Cantin, Line; Prieto, Manuel; Voyer, Normand; Salesse, Christian. "Membrane binding properties of the C-terminal segment of retinol dehydrogenase 8". Biochimica et Biophysica Acta (BBA) - Biomembranes 1863 9 (2021): 183605. http://dx.doi.org/10.1016/j.bbamem.2021.183605. Published • 10.1016/j.bbamem.2021.183605 Scanavachi, Gustavo; Coutinho, Ana; Fedorov, Alexander Andreevich; Prieto, Manuel; Melo, Ana M.; Itri, Rosangela. "Lipid Hydroperoxide Compromises the Membrane Structure Organization and Softens Bending Rigidity". Langmuir 37 33 (2021): 9952-9963. http://dx.doi.org/10.1021/acs.langmuir.1c00830. Published • 10.1021/acs.langmuir.1c00830 Santos, Tania C.B.; Saied, Essa M.; Arenz, Christoph; Fedorov, Aleksander; Prieto, Manuel; Silva, Liana C.. "The long chain base unsaturation has a stronger impact on 1-deoxy(methyl)-sphingolipids biophysical properties than the structure of its C1 functional group". Biochimica et Biophysica Acta (BBA) - Biomembranes 1863 8 (2021): 183628. http://dx.doi.org/10.1016/j.bbamem.2021.183628. Published • 10.1016/j.bbamem.2021.183628 Lopes-de-Campos, Daniela; Pereira-Leite, Catarina; Fontaine, Philippe; Coutinho, Ana; Prieto, Manuel; Sarmento, Bruno; Jakobtorweihen, Sven; Nunes, Cláudia; Reis, Salette. "Interface-Mediated Mechanism of Action—The Root of the Cytoprotective Effect of Immediate-Release Omeprazole". Journal of Medicinal Chemistry 64 8 (2021): 5171-5184. http://dx.doi.org/10.1021/acs.jmedchem.1c00251. Published • 10.1021/acs.jmedchem.1c00251 Giudici, Ana Marcela; Díaz-García, Clara; Renart, Maria Lourdes; Coutinho, Ana; Prieto, Manuel; González-Ros, José M.; Poveda, José Antonio. "Tetraoctylammonium, a Long Chain Quaternary Ammonium Blocker, Promotes a Noncollapsed, Resting-Like Inactivated State in KcsA". International Journal of Molecular Sciences 22 2 (2021): 490. http://dx.doi.org/10.3390/ijms22020490. Published • 10.3390/ijms22020490 Diaz-Garcia, Clara; Renart, Lourdes; Giudici, Marcela; Poveda, Jose A.; Fedorov, Aleksander; Gonzalez-Ros, Jose M.; Berberan-Santos, Mario N.; Prieto, Manuel; Coutinho, Ana. "Bidirectional crosstalk during the gating of KcsA revealed by homo-FRET methodologies". European Biophysics Journal (2021): https://publons.com/wos-op/publon/53799494/. Santos, Tania C. B.; Saied, Essa M.; Arenz, Christoph; Fedorov, Aleksander; Prieto, Manuel; Silva, Liana C.. "The long chain base unsaturation has a stronger impact on 1-deoxy(methyl)-sphingolipids biophysical properties than the structure of its C1 functional group". Biochimica et Biophysica Acta (BBA) - Biomembranes (2021): https://publons.com/wos-op/publon/46740452/. 10.1016/J.BBAMEM.2021.183628 Hadicke, Andre; Coutinho, Ana; Roy, Sarah; Otis, Francois; Lhor, Mustapha; Cantin, Line; Prieto, Manuel; Voyer, Normand; Salesse, Christian. "Membrane binding properties of the C-terminal segment of retinol dehydrogenase 8". Biochimica et Biophysica Acta (BBA) - Biomembranes (2021): https://publons.com/wos-op/publon/47988298/. 10.1016/J.BBAMEM.2021.183605 Lopes-de-Campos, Daniela; Pereira-Leite, Catarina; Fontaine, Philippe; Coutinho, Ana; Prieto, Manuel; Sarmento, Bruno; Jakobtorweihen, Sven; Nunes, Claudia; Reis, Salette. "Interface-Mediated Mechanism of Action-The Root of the Cytoprotective Effect of Immediate-Release Omeprazole". Journal of Medicinal Chemistry (2021): https://publons.com/wos-op/publon/44786841/. 10.1021/ACS.JMEDCHEM.1C00251 Diaz-Garcia, Clara; Lourdes Renart, Maria; Marcela Giudici, Ana; Antonio Poveda, Jose; Fedorov, Aleksander; Gonzalez-Rosa, Jose M.; Berberan-Santos, Mario Nuno; Prieto, Manuel; Coutinho, Ana. "Mapping the Conformational States of the Outer Vestibule of Kcsa, a Prokaryote Potassium Channel, using the Long Chain Quaternary Ammonium Blocker, Tetraoctylammonium". Biophysical Journal (2021): https://publons.com/wos-op/publon/53799496/. Sousa, Tania; Bernardes, Nuno; Coutinho, Ana; Prieto, Manuel; Melo, Ana M.. "Effects of the Flanking polyQ Regions and Membrane Physical Properties on Huntingtin Binding to Lipid Vesicles". Biophysical Journal (2021): https://publons.com/wos-op/publon/49396934/. 10.1016/J.BPJ.2020.11.446 Scanavachi, Gustavo; Coutinho, Ana; Fedorov, Alexander Andreevich; Prieto, Manuel; Melo, Ana M.; Itri, Rosangela. "Lipid Hydroperoxide Compromises the Membrane Structure Organization and Softens Bending Rigidity". Langmuir (2021): https://publons.com/wos-op/publon/48964730/. 10.1021/ACS.LANGMUIR.1C00830 Ventura, A.E.; Varela, A.R.P.; Dingjan, T.; Santos, T.C.B.; Fedorov, A.; Futerman, A.H.; Prieto, M.; Silva, L.C.. "Lipid domain formation and membrane shaping by C24-ceramide". Biochimica et Biophysica Acta (BBA) - Biomembranes 1862 10 (2020): 183400. http://dx.doi.org/10.1016/j.bbamem.2020.183400. Published • 10.1016/j.bbamem.2020.183400 Martinez-Calle, Marta; Prieto, Manuel; Olmeda, Bárbara; Fedorov, Aleksander; Loura, Luís M.S.; Pérez-Gil, Jesús. "Pulmonary surfactant protein SP-B nanorings induce the multilamellar organization of surfactant complexes". Biochimica et Biophysica Acta (BBA) - Biomembranes 1862 6 (2020): 183216. http://dx.doi.org/10.1016/j.bbamem.2020.183216. In press • 10.1016/j.bbamem.2020.183216 Santos, Tania C.B.; Vaz, Alexandra; Ventura, Ana E; M. Saied, Essa; Arenz, Christoph; Fedorov, Aleksander; Prieto, Manuel; Silva, Liana C. "Canonical and 1-deoxy(methyl) sphingoid bases: tackling the effect of lipid structure on membrane biophysical properties". Langmuir (2020): http://dx.doi.org/10.1021/acs.langmuir.0c01000. In press • 10.1021/acs.langmuir.0c01000 Díaz-García, Clara; Hornos, Felipe; Giudici, A. Marcela; Cámara-Artigas, Ana; Luque-Ortega, Juan Román; Arbe, Arantxa; Rizzuti, Bruno; et al. "Human importin a3 and its N-terminal truncated form, without the importin-ß-binding domain, are oligomeric species with a low conformational stability in solution". Biochimica et Biophysica Acta (BBA) - General Subjects (2020): 129609. http://dx.doi.org/10.1016/j.bbagen.2020.129609. 10.1016/j.bbagen.2020.129609 Pereira-Leite, Catarina; Jamal, Sarah K.; Almeida, João P.; Coutinho, Ana; Prieto, Manuel; Cuccovia, Iolanda M.; Nunes, Cláudia; Reis, Salette. "Neutral Diclofenac Causes Remarkable Changes in Phosphatidylcholine Bilayers: Relevance for Gastric Toxicity Mechanisms". Molecular Pharmacology 97 4 (2020): 295-303. http://dx.doi.org/10.1124/mol.119.118299. In press • 10.1124/mol.119.118299 Veiga, Ana Salome; Pinto, Sandra N.; Dias, Susana A.; Cruz, Ana F.; Mil-Homens, Dalila; Fernandes, Fabio; Valle, Javier; et al. "Unravelling the Mechanism of Action of Pepr, a Viral-Derived Membrane-Active Peptide, Against Staphylococcus Aureus Biofilms". Biophysical Journal (2020): https://publons.com/wos-op/publon/34495070/. 10.1016/J.BPJ.2019.11.2176 Ventura, A. E.; Varela, A. R. P.; Dingjan, T.; Santos, T. C. B.; Fedorov, A.; Futerman, A. H.; Prieto, M.; Silva, L. C.. "Lipid domain formation and membrane shaping by C24-ceramide". Biochimica et Biophysica Acta (BBA) - Biomembranes (2020): https://publons.com/wos-op/publon/34889469/. 10.1016/J.BBAMEM.2020.183400 Diaz-Garcia, Clara; Lourdes Renart, Maria; Marcela Giudici, A.; Antonio Poveda, Jose; Manuel Gonzalez-Ros, Jose; Berberan-Santos, Mario Nuno; Coutinho, Ana; Prieto, Manuel. "Conformational Plasticity of the KcsA Channel from Advanced TimeResolved Homo-FRET Methodologies". Biophysical Journal (2020): https://publons.com/wos-op/publon/53799504/. Santos, Tania C. B.; Vaz, Alexandra; Ventura, Ana E.; Saied, Essa M.; Arenz, Christoph; Fedorov, Aleksander; Prieto, Manuel; Silva, Liana C.. "Canonical and 1-Deoxy(methyl) Sphingoid Bases: Tackling the Effect of the Lipid Structure on Membrane Biophysical Properties". Langmuir (2020): https://publons.com/wos-op/publon/33006583/. 10.1021/ACS.LANGMUIR.0C01000 Diaz-Garcia, Clara; Hornos, Felipe; Giudici, A. Marcela; Camara-Artigas, Ana; Luque-Ortega, Juan Roman; Arbe, Arantxa; Rizzuti, Bruno; et al. "Human importin a3 and its N-terminal truncated form, without the importin-ß-binding domain, are oligomeric species with a low conformational stability in solution". Biochimica et Biophysica Acta (BBA) - General Subjects (2020): https://publons.com/wos-op/publon/31831074/. 10.1016/J.BBAGEN.2020.129609 Pinto, Sandra N; Dias, Susana A; Cruz, Ana F; Mil-Homens, Dalila; Fernandes, Fabio; Valle, Javier; Andreu, David; et al. "The mechanism of action of pepR, a viral-derived peptide, against Staphylococcus aureus biofilms". Journal of Antimicrobial Chemotherapy 74 9 (2019): 2617-2625. http://dx.doi.org/10.1093/jac/dkz223. Published • 10.1093/jac/dkz223 Renart, M. Lourdes; Giudici, A. Marcela; Poveda, José A.; Fedorov, Aleksander; Berberan-Santos, Mário N.; Prieto, Manuel; Díaz-García, Clara; González-Ros, José M.; Coutinho, Ana. "Conformational plasticity in the KcsA potassium channel pore helix revealed by homo-FRET studies". Scientific Reports 9 1 (2019): http://dx.doi.org/10.1038/s41598-019-42405-5. Published • 10.1038/s41598-019-42405-5 Neira, José L.; Díaz-García, Clara; Prieto, Manuel; Coutinho, Ana. "The C-terminal SAM domain of p73 binds to the N terminus of MDM2". Biochimica et Biophysica Acta (BBA) - General Subjects 1863 4 (2019): 760-770. http://dx.doi.org/10.1016/j.bbagen.2019.01.019. Published • 10.1016/j.bbagen.2019.01.019 Borges-Araujo, L.; Monteiro, M.; Mil-Homens, D.; Bernardes, N.; Sarmento, M. J.; Prieto, M.; Melo, M. N.; Fernandes, F.. "Impact of Ca²⁺ induced PI(4,5)P₂ clustering on the dynamics of PI(4,5)P₂ binding proteins". European Biophysics Journal (2019): https://publons.com/wos-op/publon/30362901/. Sousa, T.; Pokorna, S.; Castro, R.; Bernardes, N.; Banerjee, S.; Grobner, G.; Youle, R.; et al. "Towards and understanding of the role of Bax on bile acid-mediated cytoprotection". European Biophysics Journal (2019): https://publons.com/wos-op/publon/28542660/. Pinto, S. N.; Dias, S. A.; Cruz, A. F.; Mil-Homens, D.; Fernandes, F.; Valle, J.; Andreu, D.; et al. "Insights into the mechanism of action of pepR, a viral-derived peptide, against Staphylococcus aureus biofilms". European Biophysics Journal (2019): https://publons.com/wos-op/publon/30362900/. Ricardo, J. C.; Scanavachi, G.; Fernandes, F.; Melo, A.; Itri, R.; Coutinho, A.; Prieto, M.. "Membrane remodeling by the amyloidogenic peptide IAPP: surface crowing relevance". European Biophysics Journal (2019): https://publons.com/wos-op/publon/31764312/. Roy, Sarah; Coutinho, Ana; Cantin, Line; Gauthier, Marie-Eve; Prieto, Manuel; Gagne, Stephane M.; Salesse, Christian. "The Weak Enzymatic Activity of Truncated Lecithin Retinol Acyltransferase (LRAT) Mutants cannot be Explained by their Affinity for All-Trans Retinol". Biophysical Journal (2019): https://publons.com/wos-op/publon/40529413/. 10.1016/J.BPJ.2018.11.2582 Lourdes Renart, M.; Marcela Giudici, A.; Poveda, Jose A.; Fedorov, Aleksander; Berberan-Santos, Mario N.; Prieto, Manuel; Diaz-Garcia, Clara; Gonzalez-Ros, Jose M.; Coutinho, Ana. "Conformational plasticity in the KcsA potassium channel pore helix revealed by homo-FRET studies". Scientific Reports (2019): https://publons.com/wos-op/publon/18613484/. 10.1038/S41598-019-42405-5 Ouellette, Mathieu; Masse, Florence; Lefebvre-Demers, Mathilde; Maestracci, Quentin; Grenier, Philippe; Millar, Robert; Bertrand, Nicolas; Prieto, Manuel; Boisselier, Élodie. "Insights into gold nanoparticles as a mucoadhesive system". Scientific Reports 8 1 (2018): http://dx.doi.org/10.1038/s41598-018-32699-2. Published • 10.1038/s41598-018-32699-2 Miranda, Cláudia C.; Fernandes, Tiago G.; Pinto, Sandra N.; Prieto, Manuel; Diogo, M. Margarida; Cabral, Joaquim M.S.. "A scale out approach towards neural induction of human induced pluripotent stem cells for neurodevelopmental toxicity studies". Toxicology Letters 294 (2018): 51-60. http://dx.doi.org/10.1016/j.toxlet.2018.05.018. Published • 10.1016/j.toxlet.2018.05.018 Da Silva, Eliana S.; Moura, Nuno M. M.; Coutinho, Ana; Dražic, Goran; Teixeira, Bruno M. S.; Sobolev, Nikolai A.; Silva, Cláudia G.; et al. "ß-Cyclodextrin as a Precursor to Holey C-Doped g-C3N4 Nanosheets for Photocatalytic Hydrogen Generation". ChemSusChem 11 16 (2018): 2681-2694. http://dx.doi.org/10.1002/cssc.201801003. Published • 10.1002/cssc.201801003 Videira, Marco A. M.; Lobo, Susana A. L.; Silva, Liliana S. O.; Palmer, David J.; Warren, Martin J.; Prieto, Manuel; Coutinho, Ana; et al. "Staphylococcus aureus haem biosynthesis and acquisition pathways are linked through haem monooxygenase IsdG". Molecular Microbiology 109 3 (2018): 385-400. http://dx.doi.org/10.1111/mmi.14060. Published • 10.1111/mmi.14060 Dias, Tiago P.; Pinto, Sandra N.; Santos, Juliana I.; Fernandes, Tiago G.; Fernandes, Fábio; Diogo, Maria Margarida; Prieto, Manuel; Cabral, Joaquim M.S.. "Biophysical study of human induced Pluripotent Stem Cell-Derived cardiomyocyte structural maturation during long-term culture". Biochemical and Biophysical Research Communications 499 3 (2018): 611-617. http://dx.doi.org/10.1016/j.bbrc.2018.03.198. Published • 10.1016/j.bbrc.2018.03.198 Cabré, Elisa J.; Martínez-Calle, Marta; Prieto, Manuel; Fedorov, Alexander; Olmeda, Bárbara; Loura, Luís M. S.; Pérez-Gil, Jesús. "Homo- and hetero-oligomerization of hydrophobic pulmonary surfactant proteins SP-B and SP-C in surfactant phospholipid membranes". Journal of Biological Chemistry 293 24 (2018): 9399-9411. http://dx.doi.org/10.1074/jbc.ra117.000222. Published • 10.1074/jbc.ra117.000222 Da Silva, E.S.; Moura, N.M.M.; Neves, M.G.P.M.S.; Coutinho, A.; Prieto, M.; Silva, C.G.; Faria, J.L.. "Novel hybrids of graphitic carbon nitride sensitized with free-base meso-tetrakis(carboxyphenyl) porphyrins for efficient visible light photocatalytic hydrogen production". Applied Catalysis B: Environmental 221 (2018): 56-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-85029184428&partnerID=MN8TOARS. 10.1016/j.apcatb.2017.08.079 Dao, T. P. T.; Fernandes, F.; Fauquignon, M.; Ibarboure, E.; Prieto, M.; Le Meins, J. F.; T. P. T. Dao; et al. "The combination of block copolymers and phospholipids to form giant hybrid unilamellar vesicles (GHUVs) does not systematically lead to “intermediate” membrane properties". Soft Matter 14 31 (2018): 6476-6484. http://dx.doi.org/10.1039/c8sm00547h. Published • 10.1039/c8sm00547h Ouellette, Mathieu; Masse, Florence; Lefebvre-Demers, Mathilde; Maestracci, Quentin; Grenier, Philippe; Millar, Robert; Bertrand, Nicolas; Prieto, Manuel; Boisselier, Elodie. "Insights into gold nanoparticles as a mucoadhesive system". Scientific Reports (2018): https://publons.com/wos-op/publon/2475028/. 10.1038/S41598-018-32699-2 Pinto, Sandra N.; Dias, Susana Almeida; Cruz, Ana F.; Mil-Homens, Dalila; Fernandes, Fabio; Valle, Javier; Andreu, David; et al. "Activity of the viral-derived peptide pepR against Staphylococcus aureus biofilms and insights into the mechanism of action". Journal of Peptide Science (2018): https://publons.com/wos-op/publon/16766052/. Dao TP; Brûlet A; Fernandes F; Er-Rafik M; Ferji K; Schweins R; Chapel JP; et al. "Mixing block copolymers with phospholipids at the nanoscale: from hybrid polymer/lipid worm-like micelles to vesicles presenting lipid nano-domains.". (2017): http://europepmc.org/abstract/med/28128560. 10.1021/acs.langmuir.6b04478 Dao TP; Fernandes F; Ibarboure E; Ferji K; Prieto M; Sandre O; Le Meins JF. "Modulation of phase separation at the micron scale and nanoscale in giant polymer/lipid hybrid unilamellar vesicles (GHUVs).". (2017): http://europepmc.org/abstract/med/27991638. 10.1039/c6sm01625a Dao, T.P.T.; Fauquignon, M.; Fernandes, F.; Ibarboure, E.; Vax, A.; Prieto, M.; Le Meins, J.F.. "Membrane properties of giant polymer and lipid vesicles obtained by electroformation and pva gel-assisted hydration methods". Colloids and Surfaces A: Physicochemical and Engineering Aspects 533 (2017): 347-353. http://www.scopus.com/inward/record.url?eid=2-s2.0-85029356087&partnerID=MN8TOARS. 10.1016/j.colsurfa.2017.09.005 Sarmento, M.J.; Coutinho, A.; Fedorov, A.; Prieto, M.; Fernandes, F.. "Membrane Order Is a Key Regulator of Divalent Cation-Induced Clustering of PI(3,5)P2and PI(4,5)P2". Langmuir 33 43 (2017): 12463-12477. http://www.scopus.com/inward/record.url?eid=2-s2.0-85032616471&partnerID=MN8TOARS. 10.1021/acs.langmuir.7b00666 Hadicke, A.; Cantin, L.; Lhor, M.; Coutinho, A. I. A.; Prieto, M.; Salesse, C.. "Membrane binding of betinoldehydrogenase 8 (RDH8) and of its C-terminal segment". European Biophysics Journal (2017): https://publons.com/wos-op/publon/64266628/. Renart, M. L.; Giudici, M.; Poveda, J. A.; Fedorov, A.; Berberan-Santos, M. N.; Prieto, M.; Gonzalez-Ros, J. M.; Coutinho, A.. "Probing conformational changes of K plus channel KcsA by time-resolved homo-FRET studies". European Biophysics Journal (2017): https://publons.com/wos-op/publon/18598047/. Sousa, T.; Pokorna, S.; Bernardes, N.; Castro, R. E.; Hof, M.; Banerjee, S.; Youle, R. J.; et al. "Cytoprotective bile acids inhibit Bax activity". European Biophysics Journal (2017): https://publons.com/wos-op/publon/12145843/. Ricardo, J. C.; Fedorov, A.; Fernandes, F.; Prieto, M.; Coutinho, A. I. A.. "Structure and dynamics of amylin-lipid mixed fibers from advanced fluorescence microscopy". European Biophysics Journal (2017): https://publons.com/wos-op/publon/59075765/. Varela AR; Ventura AE; Carreira AC; Fedorov A; Futerman AH; Prieto M; Silva LC. "Pathological levels of glucosylceramide change the biophysical properties of artificial and cell membranes.". (2016): http://europepmc.org/abstract/med/27905603. 10.1039/c6cp07227e Varela AR; Couto AS; Fedorov A; Futerman AH; Prieto M; Silva LC. "Glucosylceramide Reorganizes Cholesterol-Containing Domains in a Fluid Phospholipid Membrane.". (2016): http://europepmc.org/abstract/med/26840726. 10.1016/j.bpj.2015.12.019 Sarmento, M.J.; Pinto, S.N.; Coutinho, A.; Prieto, M.; Fernandes, F.. "Accurate quantification of inter-domain partition coefficients in GUVs exhibiting lipid phase coexistence". RSC Advances 6 71 (2016): 66641-66649. http://www.scopus.com/inward/record.url?eid=2-s2.0-84979587338&partnerID=MN8TOARS. 10.1039/c6ra13170k Fernandes F; Coutinho A; Prieto M; Loura LM. "Electrostatically driven lipid-protein interaction: Answers from FRET.". (2015): http://europepmc.org/abstract/med/25769805. 10.1016/j.bbamem.2015.02.023 Dao, T.P.T.; Fernandes, F.; Er-Rafik, M.; Salva, R.; Schmutz, M.; Brûlet, A.; Prieto, M.; Sandre, O.; Le Meins, J.-F.. "Phase separation and nanodomain formation in hybrid polymer/lipid vesicles". ACS Macro Letters 4 2 (2015): 182-186. http://www.scopus.com/inward/record.url?eid=2-s2.0-84923196880&partnerID=MN8TOARS. 10.1021/mz500748f Sousa, T.; Castro, R.E.; Pinto, S.N.; Coutinho, A.; Lucas, S.D.; Moreira, R.; Rodrigues, C.M.P.; et al. "Deoxycholic acid modulates cell death signaling through changes in mitochondrial membrane properties". Journal of Lipid Research 56 11 (2015): 2158-2171. http://www.scopus.com/inward/record.url?eid=2-s2.0-84948844490&partnerID=MN8TOARS. 10.1194/jlr.M062653 Renart, M. L.; Giudici, M.; Montoya, E.; Poveda, J. A.; Fedorov, A.; Berberan-Santos, M. N.; Prieto, M.; Gonzalez-Ros, J. M.; Coutinho, A.. "Homo-FRET studies of K plus channel KcsA: a closer look to selectivity filter dynamics and regulation". European Biophysics Journal (2015): https://publons.com/wos-op/publon/6116283/. Melo, A. M.; Fedorov, A.; Prieto, M.; Coutinho, A.. "Quantitative characterization of membrane assembly of amphitropic proteins by homo-FRET measurements". European Biophysics Journal (2015): https://publons.com/wos-op/publon/61647080/. Ricardo, J. C.; Caldas, P.; Fedorov, A.; Fernandes, F.; Prieto, M.; Coutinho, A.. "Calcitonin oligomerization in solution and in the presence of anionic lipid vesicles". European Biophysics Journal (2015): https://publons.com/wos-op/publon/59075760/. Sarmento, M. J.; Coutinho, A.; Prieto, M.; Fernandes, F.. "FRET analysis of nanoscale organization of PI(4,5)P2 at the plasma membrane of living cells". European Biophysics Journal (2015): https://publons.com/wos-op/publon/18722287/. Le Meins, J. F.; Dao, Tuyen; Salva, Romain; Fernandes, Fabio; Brulet, Annie; Schmutz, Marc; Er-Rafik, Meriem; et al. "Phase separation in hybrid polymer/lipid vesicles: a rational to obtain lipid or polymer raft-like nanodomains". Abstracts of Papers of the American Chemical Society (2015): https://publons.com/wos-op/publon/16766053/. Sousa, Tania; Coutinho, Ana; Banerjee, Soojay; Castro, Rui; Youle, Richard; Rodrigues, Cecilia; Prieto, Manuel; Fernandes, Fabio. "Binding of Pro-Apoptotic Protein Bax to Cytoprotective UDCA and TUDCA". Biophysical Journal (2015): https://publons.com/wos-op/publon/30227179/. 10.1016/J.BPJ.2014.11.3313 Sarmento, Maria Joao; Coutinho, Ana; Prieto, Manuel; Fernandes, Fabio. "Analysis of PI(4,5)P2 Lateral Organization at the Plasma Membrane of Living Cells Through FRET". Biophysical Journal (2015): https://publons.com/wos-op/publon/18722291/. 10.1016/J.BPJ.2014.11.1873 de Sousa, Tania Patricia Marques; Castro, Rui E.; Pinto, Sandra N.; Coutinho, Ana; Lucas, Susana D.; Moreira, Rui; Rodrigues, Cecilia M. P.; Prieto, Manuel; Fernandes, Fabio. "The Cytotoxic Bile Acid DCA Modulates Apoptotic Signalling through Alteration of Mitochondrial Membrane Properties". Biophysical Journal (2015): https://publons.com/wos-op/publon/6375382/. 10.1016/J.BPJ.2014.11.1342 Melo, Ana M.; Fedorov, A.; Prieto, M.; Coutinho, Ana. "Oligomer Stoichiometry of Membrane-Bound Proteins Involved in a Cooperative Partition Equilibrium: A Homo-FRET Study". Biophysical Journal (2015): https://publons.com/wos-op/publon/47919833/. 10.1016/J.BPJ.2014.11.545 Amaro M; Šachl R; Jurkiewicz P; Coutinho A; Prieto M; Hof M. "Time-resolved fluorescence in lipid bilayers: selected applications and advantages over steady state.". (2014): http://europepmc.org/abstract/med/25517142. 10.1016/j.bpj.2014.10.058 Melo AM; Fedorov A; Prieto M; Coutinho A. "Exploring homo-FRET to quantify the oligomer stoichiometry of membrane-bound proteins involved in a cooperative partition equilibrium.". (2014): http://europepmc.org/abstract/med/24722583. 10.1039/c4cp00060a Suárez-Germà C; Hernández-Borrell J; Prieto M; Loura LM. "Modeling FRET to investigate the selectivity of lactose permease of Escherichia coli for lipids.". (2014): http://europepmc.org/abstract/med/24826799. 10.3109/09687688.2014.915351 Castro BM; Prieto M; Silva LC. "Ceramide: a simple sphingolipid with unique biophysical properties.". (2014): http://europepmc.org/abstract/med/24513486. 10.1016/j.plipres.2014.01.004 Varela AR; Gonçalves da Silva AM; Fedorov A; Futerman AH; Prieto M; Silva LC. "Influence of intracellular membrane pH on sphingolipid organization and membrane biophysical properties.". (2014): http://europepmc.org/abstract/med/24654655. 10.1021/la5003397 Sarmento MJ; Coutinho A; Fedorov A; Prieto M; Fernandes F. "Ca(2+) induces PI(4,5)P2 clusters on lipid bilayers at physiological PI(4,5)P2 and Ca(2+) concentrations.". (2014): http://europepmc.org/abstract/med/24316170. 10.1016/j.bbamem.2013.11.020 Melo AM; Loura LM; Fernandes F; Villalaín J; Prieto M; Coutinho A. "Electrostatically driven lipid-lysozyme mixed fibers display a multilamellar structure without amyloid features.". (2014): http://europepmc.org/abstract/med/24651998. 10.1039/c3sm52586d Melo, A.M.; Loura, L.M.S.; Fernandes, F.; Villalán, J.; Prieto, M.; Coutinho, A.. "Erratum: Electrostatically driven lipid-lysozyme mixed fibers display a multilamellar structure without amyloid features (Soft Matter (2014) 10 DOI: 10.1039/C3SM52586D)". Soft Matter 10 33 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84905457632&partnerID=MN8TOARS. Pinto, S.N.; Laviad, E.L.; Stiban, J.; Kelly, S.L.; Merrill Jr., A.H.; Prieto, M.; Futerman, A.H.; Silva, L.C.. "Changes in membrane biophysical properties induced by sphingomyelinase depend on the sphingolipid N -acyl chain". Journal of Lipid Research 55 1 (2014): 53-61. http://www.scopus.com/inward/record.url?eid=2-s2.0-84891350435&partnerID=MN8TOARS. 10.1194/jlr.M042002 Melo AM; Prieto M; Coutinho A; Melo, A.M.; Prieto, M.; Coutinho, A.. "Quantifying lipid-protein interaction by fluorescence correlation spectroscopy (FCS).". Methods in Molecular Biology 1076 (2014): 575-595. http://europepmc.org/abstract/med/24108645. 10.1007/978-1-62703-649-8_26 Melo, Ana M.; Loura, Luis M. S.; Fernandes, Fabio; Villalain, Jose; Prieto, Manuel; Coutinho, Ana. "Electrostatically driven lipid-lysozyme mixed fibers display a multilamellar structure without amyloid features (vol 10, pg 840, 2014)". Soft Matter (2014): https://publons.com/wos-op/publon/58671202/. Sousa, T.; Pinto, S.; Lucas, S. D.; Moreira, R.; Coutinho, A.; Castro, R. E.; Rodrigues, C. M.; Prieto, M.; Fernandes, F.. "INTERACTION OF APOPTOTIC AND CYTOPROTECTIVE BILE ACIDS WITH BIOMEMBRANES". Journal of Hepatology (2014): https://publons.com/wos-op/publon/6375361/. 10.1016/S0168-8278(14)60279-8 Castro, B.M.; Fedorov, A.; Hornillos, V.; Delgado, J.; Acuña, A.U.; Mollinedo, F.; Prieto, M.. "Edelfosine and miltefosine effects on lipid raft properties: Membrane biophysics in cell death by antitumor lipids". Journal of Physical Chemistry B 117 26 (2013): 7929-7940. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879749224&partnerID=MN8TOARS. 10.1021/jp401407d Pinto, S.N.; Fernandes, F.; Fedorov, A.; Futerman, A.H.; Silva, L.C.; Prieto, M.. "A combined fluorescence spectroscopy, confocal and 2-photon microscopy approach to re-evaluate the properties of sphingolipid domains". Biochimica et Biophysica Acta - Biomembranes 1828 9 (2013): 2099-2110. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879228705&partnerID=MN8TOARS. 10.1016/j.bbamem.2013.05.011 Suárez-Germà, C.; Loura, L.M.S.; Prieto, M.; Domènech, O.; Campanera, J.M.; Montero, M.T.; Hernández-Borrell, J.. "Phospholipid-lactose permease interaction as reported by a head-labeled pyrene phosphatidylethanolamine: A FRET study". Journal of Physical Chemistry B 117 22 (2013): 6741-6748. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879150730&partnerID=MN8TOARS. 10.1021/jp402152n Melo, A.M.; Ricardo, J.C.; Fedorov, A.; Prieto, M.; Coutinho, A.. "Fluorescence detection of lipid-induced oligomeric intermediates involved in lysozyme "amyloid-like" fiber formation driven by anionic membranes". Journal of Physical Chemistry B 117 10 (2013): 2906-2917. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875136314&partnerID=MN8TOARS. 10.1021/jp310396v Varela, A.R.P.; Gonçalves Da Silva, A.M.P.S.; Fedorov, A.; Futerman, A.H.; Prieto, M.; Silva, L.C.. "Effect of glucosylceramide on the biophysical properties of fluid membranes". Biochimica et Biophysica Acta - Biomembranes 1828 3 (2013): 1122-1130. http://www.scopus.com/inward/record.url?eid=2-s2.0-84872166522&partnerID=MN8TOARS. 10.1016/j.bbamem.2012.11.018 Loura, Luis M S; Prieto, Manuel; Fernandes, Fabio. "Forster resonance energy transfer as a tool for quantification of protein-lipid selectivity.". Methods in molecular biology (Clifton, N.J.) 974 (2013): 219-32. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:23404278&KeyUID=MEDLINE:23404278. 10.1007/978-1-62703-275-9_10 Mello-Vieira, Joao; Sousa, Tania; Coutinho, Ana; Fedorov, Aleksander; Lucas, Susana D.; Moreira, Rui; Castro, Rui E.; et al. "Cytotoxic bile acids, but not cytoprotective species, inhibit the ordering effect of cholesterol in model membranes at physiologically active concentrations". Biochimica Et Biophysica Acta-Biomembranes 1828 9 (2013): 2152-2163. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000322353900016&KeyUID=WOS:000322353900016. 10.1016/j.bbamem.2013.05.021 Varela, A. R.; Futerman, A. H.; Prieto, M.; Silva, L. C.. "Glusosylceramide effect on membrane properties: Impact on Gaucher Disease". European Biophysics Journal (2013): https://publons.com/wos-op/publon/45513907/. Pinto, S. N.; Varela, A. R.; Laviad, E. L.; Futerman, A. H.; Prieto, M.; Silva, L. C.. "Ceramide activates endocytosis and forms ordered intracellular lipid domains in response to TNF-a". European Biophysics Journal (2013): https://publons.com/wos-op/publon/45513900/. Ventura, A. E.; Varela, A. R.; Pinto, S. N.; Silva, A. M.; Prieto, M.; Silva, L. C.. "Lipid domain formation and membrane shaping by C24:0-ceramide". European Biophysics Journal (2013): https://publons.com/wos-op/publon/39960163/. Sarmento, M. J.; Coutinho, A.; Fedorov, A.; Prieto, M.; Fernandes, F.. "PI(4,5)P2 acts as a lipid calcium sensor in the presence of physiological calcium concentrations". European Biophysics Journal (2013): https://publons.com/wos-op/publon/18722289/. Castro, B.M.; De Almeida, R.F.M.; Fedorov, A.; Prieto, M.. "The photophysics of a Rhodamine head labeled phospholipid in the identification and characterization of membrane lipid phases". Chemistry and Physics of Lipids 165 3 (2012): 311-319. http://www.scopus.com/inward/record.url?eid=2-s2.0-84858723355&partnerID=MN8TOARS. 10.1016/j.chemphyslip.2012.02.007 Singh, A.; Wang, H.; Silva, L.C.; Na, C.; Prieto, M.; Futerman, A.H.; Luberto, C.; Del Poeta, M.. "Methylation of glycosylated sphingolipid modulates membrane lipid topography and pathogenicity of Cryptococcus neoformans". Cellular Microbiology 14 4 (2012): 500-516. http://www.scopus.com/inward/record.url?eid=2-s2.0-84862818711&partnerID=MN8TOARS. 10.1111/j.1462-5822.2011.01735.x Cabré, E.J.; Loura, L.M.S.; Fedorov, A.; Perez-Gil, J.; Prieto, M.. "Topology and lipid selectivity of pulmonary surfactant protein SP-B in membranes: Answers from fluorescence". Biochimica et Biophysica Acta - Biomembranes 1818 7 (2012): 1717-1725. http://www.scopus.com/inward/record.url?eid=2-s2.0-84859920393&partnerID=MN8TOARS. Published • 10.1016/j.bbamem.2012.03.008 Suárez-Germà, C.; Loura, L.M.S.; Prieto, M.; Domènech, O.; Montero, M.T.; Rodríguez-Banqueri, A.; Vázquez-Ibar, J.L.; Hernández-Borrell, J.. "Membrane protein-lipid selectivity: Enhancing sensitivity for modeling FRET data". Journal of Physical Chemistry B 116 8 (2012): 2438-2445. http://www.scopus.com/inward/record.url?eid=2-s2.0-84857696873&partnerID=MN8TOARS. 10.1021/jp2105665 Esquembre, R.; Pinto, S.N.; Poveda, J.A.; Prieto, M.; Mateo, C.R.. "Immobilization and characterization of giant unilamellar vesicles (GUVs) within porous silica glasses". Soft Matter 8 2 (2012): 408-417. http://www.scopus.com/inward/record.url?eid=2-s2.0-83455168947&partnerID=MN8TOARS. 10.1039/c1sm06264f Sarmento, M.J.; Prieto, M.; Fernandes, F.. "Reorganization of lipid domain distribution in giant unilamellar vesicles upon immobilization with different membrane tethers". Biochimica et Biophysica Acta - Biomembranes 1818 11 (2012): 2605-2615. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864051779&partnerID=MN8TOARS. 10.1016/j.bbamem.2012.05.028 Fernandes, F.; Prieto, M.; Loura, L.M.S.. "Advanced FRET methodologies: Protein-lipid selectivity detection and quantification". Advances in Experimental Medicine and Biology 749 (2012): 171-185. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863730716&partnerID=MN8TOARS. 10.1007/978-1-4614-3381-1_12 Silva, Liana C.; Ben David, Oshrit; Pewzner-Jung, Yael; Laviad, Elad L.; Stiban, Johnny; Bandyopadhyay, Sibali; Merrill, Alfred H., Jr.; Prieto, Manuel; Futerman, Anthony H.. "Ablation of ceramide synthase 2 strongly affects biophysical properties of membranes". Journal of Lipid Research 53 3 (2012): 430-436. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000300880200011&KeyUID=WOS:000300880200011. 10.1194/jlr.M022715 Loura, L.M.S.; Prieto, M.. "FRET in membrane biophysics: An overview". Frontiers in Physiology 2 NOV (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-84860246622&partnerID=MN8TOARS. 10.3389/fphys.2011.00082 Pinto, S.N.; Silva, L.C.; Futerman, A.H.; Prieto, M.. "Effect of ceramide structure on membrane biophysical properties: The role of acyl chain length and unsaturation". Biochimica et Biophysica Acta - Biomembranes 1808 11 (2011): 2753-2760. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052265605&partnerID=MN8TOARS. 10.1016/j.bbamem.2011.07.023 Madeira, C.; Loura, L.M.S.; Aires-Barros, M.R.; Prieto, M.. "Fluorescence methods for lipoplex characterization". Biochimica et Biophysica Acta - Biomembranes 1808 11 (2011): 2694-2705. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052261029&partnerID=MN8TOARS. 10.1016/j.bbamem.2011.07.020 Castro, B.M.; De Almeida, R.F.M.; Goormaghtigh, E.; Fedorov, A.; Prieto, M.. "Organization and dynamics of fas transmembrane domain in raft membranes and modulation by ceramide". Biophysical Journal 101 7 (2011): 1632-1641. http://www.scopus.com/inward/record.url?eid=2-s2.0-80053364521&partnerID=MN8TOARS. 10.1016/j.bpj.2011.08.022 Melo, A.M.; Prieto, M.; Coutinho, A.. "The effect of variable liposome brightness on quantifying lipid-protein interactions using fluorescence correlation spectroscopy". Biochimica et Biophysica Acta - Biomembranes 1808 10 (2011): 2559-2568. http://www.scopus.com/inward/record.url?eid=2-s2.0-80051786035&partnerID=MN8TOARS. 10.1016/j.bbamem.2011.06.001 Coutinho, A.; Loura, L.M.S.; Prieto, M.. "FRET studies of lipid-protein aggregates related to amyloid-like fibers". Journal of Neurochemistry 116 5 (2011): 696-701. http://www.scopus.com/inward/record.url?eid=2-s2.0-79851495279&partnerID=MN8TOARS. 10.1111/j.1471-4159.2010.07000.x Prieto, Manuel; F. Fernandes; L. Loura. "FRET in membranes: Förster resonance energy transfer in membranes: application to lipid domains and protein-lipid selectivity". Canal BQ 8 (2011): 12-19. Published Melo, Ana M.; Coutinho, Ana; Prieto, Manuel. "Quantifying Protein Binding to Lipid Vesicles Using Fluorescence Correlation Spectroscopy (FCS)". Biophysical Journal (2011): https://publons.com/wos-op/publon/57107788/. Picas, L.; Suárez-Germà, C.; Montero, M.T.; Vázquez-Ibar, J.L.; Hernández-Borrell, J.; Prieto, M.; Loura, L.M.S.. "Lactose permease lipid selectivity using Förster resonance energy transfer". Biochimica et Biophysica Acta - Biomembranes 1798 9 (2010): 1707-1713. http://www.scopus.com/inward/record.url?eid=2-s2.0-77954657104&partnerID=MN8TOARS. 10.1016/j.bbamem.2010.05.012 Pewzner-Jung, Y.; Park, H.; Laviad, E.L.; Silva, L.C.; Lahiri, S.; Stiban, J.; Erez-Roman, R.; et al. "A critical role for ceramide synthase 2 in liver homeostasis: I. alterations in lipid metabolic pathways". Journal of Biological Chemistry 285 14 (2010): 10902-10910. http://www.scopus.com/inward/record.url?eid=2-s2.0-77951234061&partnerID=MN8TOARS. 10.1074/jbc.M109.077594 Loura, L.M.S.; Fernandes, F.; Prieto, M.. "Membrane microheterogeneity: Förster resonance energy transfer characterization of lateral membrane domains". European Biophysics Journal 39 4 (2010): 589-607. http://www.scopus.com/inward/record.url?eid=2-s2.0-77952098602&partnerID=MN8TOARS. 10.1007/s00249-009-0547-5 Loura, L.M.S.; Prieto, M.; Fernandes, F.. "Quantification of protein-lipid selectivity using FRET". European Biophysics Journal 39 4 (2010): 565-578. http://www.scopus.com/inward/record.url?eid=2-s2.0-77952093185&partnerID=MN8TOARS. 10.1007/s00249-009-0532-z de Almeida, R.F.M.; Loura, L.M.S.; Prieto, M.. "Membrane lipid domains and rafts: current applications of fluorescence lifetime spectroscopy and imaging". Chemistry and Physics of Lipids 157 2 (2009): 61-77. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149469939&partnerID=MN8TOARS. 10.1016/j.chemphyslip.2008.07.011 Loura, Luis M. S.; Prieto, Manuel. "Characterization of Peptide-Induced Morphological Alterations in Membranes by Fluorescence Resonance Energy Transfer". Protein and Peptide Letters 16 7 (2009): 726-735. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000268219500004&KeyUID=WOS:000268219500004. 10.2174/092986609788681698 Silva, L.C.; Futerman, A.H.; Prieto, M.. "Lipid raft composition modulates sphingomyelinase activity and ceramide-induced membrane physical alterations". Biophysical Journal 96 8 (2009): 3210-3222. http://www.scopus.com/inward/record.url?eid=2-s2.0-67649410710&partnerID=MN8TOARS. 10.1016/j.bpj.2008.12.3923 Castro, B.M.; Silva, L.C.; Fedorov, A.; de Almeida, R.F.M.; Prieto, M.. "Cholesterol-rich fluid membranes solubilize ceramide domains: Implications for the structure and dynamics of mammalian intracellular and plasma membranes". Journal of Biological Chemistry 284 34 (2009): 22978-22987. http://www.scopus.com/inward/record.url?eid=2-s2.0-69249087317&partnerID=MN8TOARS. 10.1074/jbc.M109.026567 Scolari, S.; Engel, S.; Krebs, N.; Piazzo, A.P.; De Almeida, R.F.M.; Prieto, M.; Veit, M.; Hermann, A.. "Lateral distribution of the transmembrane domain of influenza virus hemagglutinin revealed by time-resolved fluorescence imaging". Journal of Biological Chemistry 284 23 (2009): 15708-15716. http://www.scopus.com/inward/record.url?eid=2-s2.0-67650103574&partnerID=MN8TOARS. 10.1074/jbc.M900437200 Guillén, J.; De Almeida, R.F.M.; Prieto, M.; Villalaín, J.. "Interaction of a peptide corresponding to the loop domain of the S2 SARS-CoV virus protein with model membranes". Molecular Membrane Biology 26 4 (2009): 236-248. http://www.scopus.com/inward/record.url?eid=2-s2.0-67650317982&partnerID=MN8TOARS. 10.1080/09687680902926203 Loura, L.M.S.; de Almeida, R.F.M.; Silva, L.C.; Prieto, M.. "FRET analysis of domain formation and properties in complex membrane systems". Biochimica et Biophysica Acta - Biomembranes 1788 1 (2009): 209-224. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149201036&partnerID=MN8TOARS. 10.1016/j.bbamem.2008.10.012 Loura, L.M.S.; Prieto, M.. "Characterization of peptide-induced morphological alterations in membranes by fluorescence resonance energy transfer". Protein and Peptide Letters 16 7 (2009): 726-735. http://www.scopus.com/inward/record.url?eid=2-s2.0-67849104239&partnerID=MN8TOARS. 10.2174/092986609788681698 Castro, Bruno M.; de Almeida, Rodrigo F. M.; Silva, Liana C.; Prieto, Manuel. "Interactions of Ceramide and Sphingomyelin Quantified in Mixtures with an Unsaturated Phosphatidylcholine". Biophysical Journal (2009): https://publons.com/wos-op/publon/18228137/. 10.1016/J.BPJ.2008.12.1792 Baumgart, Florian; Loura, Luis M. S.; Prieto, Manuel; Gil, Jesus Pierez. "Pulmonary Surfactant Protein C Reduces the Size of Liquid Ordered Domains in a Ternary Membrane Model System". Biophysical Journal (2009): https://publons.com/wos-op/publon/58671201/. 10.1016/J.BPJ.2008.12.3219 Pinto, Sandra N.; Silva, Liana C.; de Almeida, Rodrigo F. M.; Prieto, Manuel. "Interdigitation, Domains and Morphology, in Membranes of the Chain Asymmetric C24:1 Ceramide". Biophysical Journal (2009): https://publons.com/wos-op/publon/18228142/. 10.1016/J.BPJ.2008.12.1789 Guillén, J.; De Almeida, R.F.M.; Prieto, M.; Villalaín, J.. "Structural and dynamic characterization of the interaction of the putative fusion peptide of the S2 SARS-CoV virus protein with lipid membranes". Journal of Physical Chemistry B 112 23 (2008): 6997-7007. http://www.scopus.com/inward/record.url?eid=2-s2.0-48549104349&partnerID=MN8TOARS. 10.1021/jp7118229 Hesselink, R.W.; Fedorov, A.; Hemminga, M.A.; Prieto, M.. "Membrane-bound peptides from V-ATPase subunit a do not interact with an indole-type inhibitor". Journal of Peptide Science 14 4 (2008): 383-388. http://www.scopus.com/inward/record.url?eid=2-s2.0-42149092185&partnerID=MN8TOARS. 10.1002/psc.980 Madeira, C.; Loura, L.M.S.; Prieto, M.; Fedorov, A.; Aires-Barros, M.R.. "Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET". BMC Biotechnology 8 (2008): http://www.scopus.com/inward/record.url?eid=2-s2.0-41349094583&partnerID=MN8TOARS. 10.1186/1472-6750-8-20 Holt, A.; De Almeida, R.F.M.; Nyholm, T.K.M.; Loura, L.M.S.; Daily, A.E.; Staffhorst, R.W.H.M.; Rijkers, D.T.S.; et al. "Is there a preferential interaction between cholesterol and tryptophan residues in membrane proteins?". Biochemistry 47 8 (2008): 2638-2649. http://www.scopus.com/inward/record.url?eid=2-s2.0-39749193962&partnerID=MN8TOARS. 10.1021/bi702235k Pastor, I.; Prieto, M.; Mateo, C.R.. "Effect of sol-gel confinement on the structural dynamics of the enzyme bovine Cu,Zn superoxide dismutase". Journal of Physical Chemistry B 112 47 (2008): 15021-15028. http://www.scopus.com/inward/record.url?eid=2-s2.0-57449094610&partnerID=MN8TOARS. 10.1021/jp805368s Coutinho, A.; Loura, L.M.S.; Fedorov, A.; Prieto, M.. "Pinched multilamellar structure of aggregates of lysozyme and phosphatidylserine-containing membranes revealed by FRET". Biophysical Journal 95 10 (2008): 4726-4736. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149288322&partnerID=MN8TOARS. 10.1529/biophysj.108.134379 Goñi, F.M.; Alonso, A.; Bagatolli, L.A.; Brown, R.E.; Marsh, D.; Prieto, M.; Thewalt, J.L.. "Phase diagrams of lipid mixtures relevant to the study of membrane rafts". Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids 1781 11-12 (2008): 665-684. http://www.scopus.com/inward/record.url?eid=2-s2.0-57649107937&partnerID=MN8TOARS. 10.1016/j.bbalip.2008.09.002 Pinto, S.N.; Silva, L.C.; De Almeida, R.F.M.; Prieto, M.. "Membrane domain formation, interdigitation, and morphological alterations induced by the very long chain asymmetric C24:1 ceramide". Biophysical Journal 95 6 (2008): 2867-2879. http://www.scopus.com/inward/record.url?eid=2-s2.0-55549135378&partnerID=MN8TOARS. 10.1529/biophysj.108.129858 Fernandes, F.; Loura, L.M.S.; Chichón, F.J.; Carrascosa, J.L.; Fedorov, A.; Prieto, M.. "Role of helix 0 of the N-BAR domain in membrane curvature generation". Biophysical Journal 94 8 (2008): 3065-3073. http://www.scopus.com/inward/record.url?eid=2-s2.0-43149102504&partnerID=MN8TOARS. 10.1529/biophysj.107.113118 Bastos, M.; Bai, G.; Gomes, P.; Andreu, D.; Goormaghtigh, E.; Prieto, M.. "Energetics and partition of two cecropin-melittin hybrid peptides to model membranes of different composition". Biophysical Journal 94 6 (2008): 2128-2141. http://www.scopus.com/inward/record.url?eid=2-s2.0-44049094436&partnerID=MN8TOARS. 10.1529/biophysj.107.119032 Fernandes, Fábio; Neves, Patrícia; Gameiro, Paula; Loura, Luís M. S.; Prieto, Manuel; Fernandes, F.; Neves, P.; et al. "Ciprofloxacin interactions with bacterial protein OmpF: Modelling of FRET from a multi-tryptophan protein trimer". Biochimica et Biophysica Acta - Biomembranes 1768 11 (2007): 2822-2830. http://hdl.handle.net/10316/5850. 10.1016/j.bbamem.2007.07.016 Loura, L.M.S.; Prieto, M.; Loura, Luis M S; Prieto, Manuel. "Fluorescence resonance energy transfer to characterize cholesterol-induced domains". Methods in Molecular Biology 400 (2007): 489-501. http://www.scopus.com/inward/record.url?eid=2-s2.0-36549051638&partnerID=MN8TOARS. 10.1385/1-59745-519-9:489 Poveda, J.A.; Fernández-Ballester, G.; Prieto, M.; Neira, J.L.. "Dynamics of tryptophan in the histidine-containing phosphocarrier protein of Streptomyces coelicolor: Evidence of multistate equilibrium unfolding". Biochemistry 46 24 (2007): 7252-7260. http://www.scopus.com/inward/record.url?eid=2-s2.0-34250845865&partnerID=MN8TOARS. 10.1021/bi7002923 Mano, M.; Henriques, A.; Paiva, A.; Prieto, M.; Gavilanes, F.; Simões, S.; Pedroso de Lima, M.C.; et al. "Interaction of S413-PV cell penetrating peptide with model membranes: Relevance to peptide translocation across biological membranes". Journal of Peptide Science 13 5 (2007): 301-313. http://www.scopus.com/inward/record.url?eid=2-s2.0-34248347468&partnerID=MN8TOARS. 10.1002/psc.842 de Almeida, Rodrigo F. M.; Borst, Jan Willem; Fedorov, Alexandre; Prieto, Manuel; Visser, Antoine J. W. G.; Borst, JanWillem; Fedorov, Alexander; Visser, Antonie J. W. G.. "Complexity of lipid domains and rafts in giant unilamellar vesicles revealed by combining imaging, microscopic and macroscopic time-resolved fluorescence". Biophysical Journal 93 2 (2007): 356A-356A. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000243972402211&KeyUID=WOS:000243972402211. 10.1529/biophysj.106.098822 Castro, B.M.; De Almeida, R.F.M.; Silva, L.C.; Fedorov, A.; Prieto, M.. "Formation of ceramide/sphingomyelin gel domains in the presence of an unsaturated phospholipid: A quantitative multiprobe approach". Biophysical Journal 93 5 (2007): 1639-1650. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548629303&partnerID=MN8TOARS. 10.1529/biophysj.107.107714 Madeira, C.; Loura, L.M.S.; Prieto, M.; Fedorov, A.; Aires-Barros, M.R.. "Liposome complexation efficiency monitored by FRET: Effect of charge ratio, helper lipid and plasmid size". European Biophysics Journal 36 6 (2007): 609-620. http://www.scopus.com/inward/record.url?eid=2-s2.0-34347399346&partnerID=MN8TOARS. 10.1007/s00249-007-0130-x Silva, L.C.; De Almeida, R.F.M.; Castro, B.M.; Fedorov, A.; Prieto, M.. "Ceramide-domain formation and collapse in lipid rafts: Membrane reorganization by an apoptotic lipid". Biophysical Journal 92 2 (2007): 502-516. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846411845&partnerID=MN8TOARS. 10.1529/biophysj.106.091876 Fernandes, F.; Loura, L.M.S.; Fedorov, A.; Dixon, N.; Kee, T.P.; Prieto, M.; Hemminga, M.A.. "Binding assays of inhibitors towards selected V-ATPase domains". Biochimica et Biophysica Acta - Biomembranes 1758 11 (2006): 1777-1786. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750505433&partnerID=MN8TOARS. 10.1016/j.bbamem.2006.07.006 Fernandes, F.; Loura, L.M.S.; Fedorov, A.; Prieto, M.. "Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine". Journal of Lipid Research 47 7 (2006): 1521-1525. http://www.scopus.com/inward/record.url?eid=2-s2.0-33746058677&partnerID=MN8TOARS. 10.1194/jlr.M600121-JLR200 De Almeida, R.F.M.; Loura, L.M.S.; Prieto, M.; Watts, A.; Fedorov, A.; Barrantes, F.J.. "Structure and dynamics of the ¿M4 transmembrane domain of the acetylcholine receptor in lipid bilayers: Insights into receptor assembly and function". Molecular Membrane Biology 23 4 (2006): 305-315. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750481856&partnerID=MN8TOARS. 10.1080/09687860600703613 Silva, L.; Coutinho, A.; Fedorov, A.; Prieto, M.. "Competitive binding of cholesterol and ergosterol to the polyene antibiotic nystatin. A fluorescence study". Biophysical Journal 90 10 (2006): 3625-3631. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646201285&partnerID=MN8TOARS. 10.1529/biophysj.105.075408 Fernandes, F.; Loura, L.; Koehorst, R.B.M.; Dixon, N.; Kee, T.P.; Hemminga, M.A.; Prieto, M.. "Interaction of the indole class of vacuolar H+-ATPase inhibitors with lipid bilayers". Biochemistry 45 16 (2006): 5271-5279. http://www.scopus.com/inward/record.url?eid=2-s2.0-33645965883&partnerID=MN8TOARS. 10.1021/bi0522753 Loura, L.M.S.; Coutinho, A.; Silva, A.; Fedorov, A.; Prieto, M.. "Structural effects of a basic peptide on the organization of dipalmitoylphosphatidylcholine/dipalmitoylphosphatidylserine membranes: A fluorescent resonance energy transfer study". Journal of Physical Chemistry B 110 15 (2006): 8130-8141. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646432489&partnerID=MN8TOARS. 10.1021/jp055855i Silva, L.; Coutinho, A.; Fedorov, A.; Prieto, M.. "Nystatin-induced lipid vesicles permeabilization is strongly dependent on sterol structure". Biochimica et Biophysica Acta - Biomembranes 1758 4 (2006): 452-459. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646785059&partnerID=MN8TOARS. 10.1016/j.bbamem.2006.03.008 Mano, M.; Henriques, A.; Paiva, A.; Prieto, M.; Gavilanes, F.; Simões, S.; Pedroso de Lima, M.C.. "Cellular uptake of S413-PV peptide occurs upon conformational changes induced by peptide-membrane interactions". Biochimica et Biophysica Acta - Biomembranes 1758 3 (2006): 336-346. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646534241&partnerID=MN8TOARS. 10.1016/j.bbamem.2006.01.014 Silva, L.; De Almeida, R.F.M.; Fedorov, A.; Matos, A.P.A.; Prieto, M.. "Ceramide-platform formation and -induced biophysical changes in a fluid phospholipid membrane". Molecular Membrane Biology 23 2 (2006): 137-150. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646880484&partnerID=MN8TOARS. 10.1080/09687860500439474 de Almeida, Rodrigo F.M.; Loura, Luís M.S.; Fedorov, Alexander; Prieto, Manuel. "Lipid Rafts have Different Sizes Depending on Membrane Composition: A Time-resolved Fluorescence Resonance Energy Transfer Study". Journal of Molecular Biology 346 4 (2005): 1109-1120. http://dx.doi.org/10.1016/j.jmb.2004.12.026. Published • 10.1016/j.jmb.2004.12.026 Madeira, C.; Fedorov, A.; Aires-Barros, M.R.; Prieto, M.; Loura, L.M.S.. "Photophysical behavior of a dimeric cyanine dye (BOBO-1) within cationic liposomes". Photochemistry and Photobiology 81 6 (2005): 1450-1459. http://www.scopus.com/inward/record.url?eid=2-s2.0-29844457440&partnerID=MN8TOARS. 10.1562/2005-03-16-RA-464 Pascual, R.; Contreras, M.; Fedorov, A.; Prieto, M.; Villalaín, J.. "Interaction of a peptide derived from the N-heptad repeat region of gp41 Env ectodomain with model membranes. Modulation of phospholipid phase behavior". Biochemistry 44 43 (2005): 14275-14288. http://www.scopus.com/inward/record.url?eid=2-s2.0-27444434548&partnerID=MN8TOARS. 10.1021/bi050928+ de Almeida, RFM; Loura, LMS; Prieto, M; Geddes, CD; Lakowicz, JR. "Application of fluorescence to understand the interaction of peptides with binary lipid membranes". Reviews in Fluorescence 2005 2 (2005): 271-323. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=CCC&KeyUT=CCC:000228206200012&KeyUID=CCC:000228206200012. de Almeida, RFM; Loura, LMS; Prieto, M; Watts, A; Fedorov, A; Barrantes, FJ. "Cholesterol modulates the organization of the gamma M4 transmembrane domain of the muscle nicotinic acetylcholine receptor". Biophysical Journal 86 4 (2004): 2261-2272. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000220567600031&KeyUID=WOS:000220567600031. 10.1016/S0006-3495(04)74284-8 Coutinho, A.; Silva, L.; Fedorov, A.; Prieto, M.. "Cholesterol and ergosterol influence nystatin surface aggregation: Relation to pore formation". Biophysical Journal 87 5 (2004): 3264-3276. http://www.scopus.com/inward/record.url?eid=2-s2.0-16344382233&partnerID=MN8TOARS. 10.1529/biophysj.104.044883 Fernandes, F.; Loura, L.M.S.; Koehorst, R.; Spruijt, R.B.; Hemminga, M.A.; Fedorov, A.; Prieto, M.. "Quantification of protein-lipid selectivity using FRET: Application to the M13 major coat protein". Biophysical Journal 87 1 (2004): 344-352. http://www.scopus.com/inward/record.url?eid=2-s2.0-3042771646&partnerID=MN8TOARS. 10.1529/biophysj.104.040337 De Almeida, R.F.M.; Loura, L.M.S.; Prieto, M.; Watts, A.; Fedorov, A.; Barrantes, F.J.. "Cholesterol Modulates the Organization of the ¿M4 Transmembrane Domain of the Muscle Nicotinic Acetylcholine Receptor". Biophysical Journal 86 4 (2004): 2261-2272. http://www.scopus.com/inward/record.url?eid=2-s2.0-1942519443&partnerID=MN8TOARS. Fernandes, F; Loura, LMS; Prieto, M; Koehorst, R; Fernandes, F.; Loura, L.M.S.; Prieto, M.; et al. "Dependence of M13 major coat protein oligomerization and lateral segregation on bilayer composition". Biophysical Journal 85 4 (2003): 2430-2441. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000185575400032&KeyUID=WOS:000185575400032. 10.1016/S0006-3495(03)74666-9 Coutinho, A; Prieto, M; Coutinho, A.; Prieto, M.. "Cooperative partition model of nystatin interaction with phospholipid vesicles". Biophysical Journal 84 5 (2003): 3061-3078. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000183123200025&KeyUID=WOS:000183123200025. 10.1016/S0006-3495(03)70032-0 Silva, L.; Coutinho, A.; Fedorov, A.; Prieto, M.. "Solution conformation of a nitrobenzoxadiazole derivative of the polyene antibiotic nystatin: A FRET study". Journal of Photochemistry and Photobiology B: Biology 72 1-3 (2003): 17-26. http://www.scopus.com/inward/record.url?eid=2-s2.0-0344237181&partnerID=MN8TOARS. 10.1016/j.jphotobiol.2003.08.005 Loura, L.M.S.; De Almeida, R.F.M.; Prieto, M.. "Methodologies and formalisms of resonance energy transfer in biophysics. Application to membrane model systems". International Journal of Photoenergy 5 4 (2003): 223-231. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547711580&partnerID=MN8TOARS. 10.1155/S1110662X03000369 Madeira, C.; Loura, L.M.S.; Aires-Barros, M.R.; Fedorov, A.; Prieto, M.; Madeira, C; Loura, LMS; et al. "Characterization of DNA/Lipid Complexes by Fluorescence Resonance Energy Transfer". Biophysical Journal 85 5 (2003): 3106-3119. http://www.scopus.com/inward/record.url?eid=2-s2.0-0242353819&partnerID=MN8TOARS. 10.1016/S0006-3495(03)74729-8 Silva, L.; Coutinho, A.; Fedorov, A.; Prieto, M.. "Conformation and self-assembly of a nystatin nitrobenzoxadiazole derivative in lipid membranes". Biochimica et Biophysica Acta - Biomembranes 1617 1-2 (2003): 69-79. http://www.scopus.com/inward/record.url?eid=2-s2.0-0345017109&partnerID=MN8TOARS. 10.1016/j.bbamem.2003.09.004 De Almeida, R.F.M.; Fedorov, A.; Prieto, M.; de Almeida, RFM; Fedorov, A; Prieto, M. "Sphingomyelin/phosphatidylcholine/cholesterol phase diagram: Boundaries and composition of lipid rafts". Biophysical Journal 85 4 (2003): 2406-2416. http://www.scopus.com/inward/record.url?eid=2-s2.0-0141642121&partnerID=MN8TOARS. 10.1016/S0006-3495(03)74664-5 Poveda, J.A.; Prieto, M.; Encinar, J.A.; González-Ros, J.M.; Mateo, C.R.. "Intrinsic tyrosine fluorescence as a tool to study the interaction of the Shaker B "ball" peptide with anionic membranes". Biochemistry 42 23 (2003): 7124-7132. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038470046&partnerID=MN8TOARS. 10.1021/bi027183h Santos, N.C.; Prieto, M.; Castanho, M.A.R.B.. "Quantifying molecular partition into model systems of biomembranes: An emphasis on optical spectroscopic methods". Biochimica et Biophysica Acta - Biomembranes 1612 2 (2003): 123-135. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038392423&partnerID=MN8TOARS. 10.1016/S0005-2736(03)00112-3 Rodrigues, C.; Gameiro, P.; Prieto, M.; De Castro, B.. "Interaction of rifampicin and isoniazid with large unilamellar liposomes: Spectroscopic location studies". Biochimica et Biophysica Acta - General Subjects 1620 1-3 (2003): 151-159. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037451743&partnerID=MN8TOARS. 10.1016/S0304-4165(02)00528-7 Loura, L.M.S.; De Almeida, R.F.M.; Coutinho, A.; Prieto, M.. "Interaction of peptides with binary phospholipid membranes: Application of fluorescence methodologies". Chemistry and Physics of Lipids 122 1-2 (2003): 77-96. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037274881&partnerID=MN8TOARS. 10.1016/S0009-3084(02)00180-9 de Almeida, RFM; Loura, LMS; Fedorov, A; Prieto, M; De Almeida, R.F.M.; Loura, L.M.S.; Fedorov, A.; Prieto, M.. "Nonequilibrium phenomena in the phase separation of a two-component lipid bilayer". Biophysical Journal 82 2 (2002): 823-834. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000173497600025&KeyUID=WOS:000173497600025. 10.1016/S0006-3495(02)75444-1 Loura, L.M.S.; Fedorov, A.; Prieto, M.. "Exclusion of a cholesterol analog from the cholesterol-rich phase in model membranes". Biochimica et Biophysica Acta - Biomembranes 1511 2 (2001): 236-243. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035795019&partnerID=MN8TOARS. 10.1016/S0005-2736(01)00269-3 Lopes, S; Fernandes, MX; Prieto, M; Castanho, MARB; Lopes, S.; Fernandes, M.X.; Prieto, M.; Castanho, M.A.R.B.. "Orientational order of the polyene fatty acid membrane probe trans-parinaric acid in Langmuir-Blodgett multilayer films". Journal of Physical Chemistry B 105 2 (2001): 562-568. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000166490900028&KeyUID=WOS:000166490900028. 10.1021/jp0026742 Contreras, LM; de Almeida, RFM; Villalain, J; Fedorov, A; Prieto, M; Contreras, L.M.; De Almeida, R.F.M.; et al. "Interaction of alpha-melanocyte stimulating hormone with binary phospholipid membranes: Structural changes and relevance of phase behavior". Biophysical Journal 80 5 (2001): 2273-2283. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000168485800023&KeyUID=WOS:000168485800023. 10.1016/S0006-3495(01)76199-1 Loura, LMS; Fedorov, A; Prieto, M; Loura, L.M.S.; Fedorov, A.; Prieto, M.. "Fluid-fluid membrane microheterogeneity: A fluorescence resonance energy transfer study". Biophysical Journal 80 2 (2001): 776-788. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000166765100020&KeyUID=WOS:000166765100020. 10.1016/S0006-3495(01)76057-2 Loura, L.M.S.; De Almeida, R.F.M.; Prieto, M.; Loura, LMS; de Almeida, RFM; Prieto, M. "Detection and characterization of membrane microheterogeneity by resonance energy transfer". Journal of Fluorescence 11 3 (2001): 197-209. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001528446&partnerID=MN8TOARS. 10.1023/A:1012249117580 Loura, L.M.S.; Castanho, M.A.R.B.; Fedorov, A.; Prieto, M.. "A photophysical study of the polyene antibiotic filipin: Self-aggregation and filipin-ergosterol interaction". Biochimica et Biophysica Acta - Biomembranes 1510 1-2 (2001): 125-135. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035830590&partnerID=MN8TOARS. 10.1016/S0005-2736(00)00341-2 Mateo, C.R.; Prieto, M.; Micol, V.; Shapiro, S.; Villalaín, J.. "A fluorescence study of the interaction and location of (+)-totarol, a diterpenoid bioactive molecule, in model membranes". Biochimica et Biophysica Acta - Biomembranes 1509 1-2 (2000): 167-175. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034695069&partnerID=MN8TOARS. 10.1016/S0005-2736(00)00291-1 Barrantes, F.J.; Antollini, S.S.; Blanton, M.P.; Prieto, M.. "Topography of nicotinic acetylcholine receptor membrane-embedded domains". Journal of Biological Chemistry 275 48 (2000): 37333-37339. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034532112&partnerID=MN8TOARS. 10.1074/jbc.M005246200 Loura, L.M.S.; Fedorov, A.; Prieto, M.. "Partition of membrane probes in a gel/fluid two-component lipid system: A fluorescence resonance energy transfer study". Biochimica et Biophysica Acta - Biomembranes 1467 1 (2000): 101-112. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034738620&partnerID=MN8TOARS. 10.1016/S0005-2736(00)00211-X Loura, L.M.S.; Prieto, M.; Loura, LMS; Prieto, M. "Resonance energy transfer in heterogeneous planar and bilayer systems: Theory and simulation". Journal of Physical Chemistry B 104 29 (2000): 6911-6919. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034228089&partnerID=MN8TOARS. 10.1021/jp000245y Loura, L.M.S.; Fedorov, A.; Prieto, M.; Loura, LMS; Fedorov, A; Prieto, M. "Membrane probe distribution heterogeneity: A resonance energy transfer study". Journal of Physical Chemistry B 104 29 (2000): 6920-6931. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034226955&partnerID=MN8TOARS. 10.1021/jp000246q Fernandes, A.R.; Prieto, M.; Sá-Correia, I.. "Modification of plasma membrane lipid order and H+-ATPase activity as part of the response of Saccharomyces cerevisiae to cultivation under mild and high copper stress". Archives of Microbiology 173 4 (2000): 262-268. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034119202&partnerID=MN8TOARS. 10.1007/s002030000138 Castanho, M.A.R.B.; Prieto, M.; Jameson, D.M.. "The pentaene macrolide antibiotic filipin prefers more rigid DPPC bilayers: A fluorescence pressure dependence study". Biochimica et Biophysica Acta - Biomembranes 1419 1 (1999): 1-14. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032998493&partnerID=MN8TOARS. 10.1016/S0005-2736(99)00049-8 Castanho, M.A.R.B.; Prieto, M.J.E.. "Fluorescence quenching data interpretation in biological systems. The use of microscopic models for data analysis and interpretation of complex systems". Biochimica et Biophysica Acta - Biomembranes 1373 1 (1998): 1-16. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032516728&partnerID=MN8TOARS. 10.1016/S0005-2736(98)00081-9 Santos, N.C.; Ter-Ovanesyan, E.; Zasadzinski, J.A.; Prieto, M.; Castanho, M.A.R.B.; Santos, NC; Ter-Ovanesyan, E; et al. "Filipin-induced lesions in planar phospholipid bilayers imaged by atomic force microscopy". Biophysical Journal 75 4 (1998): 1869-1873. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031690831&partnerID=MN8TOARS. 10.1016/S0006-3495(98)77627-1 Santos, N.C.; Prieto, M.; Castanho, M.A.R.B.. "Interaction of the major epitope region of HIV protein gp41 with membrane model systems. A fluorescence spectroscopy study". Biochemistry 37 24 (1998): 8674-8682. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032537597&partnerID=MN8TOARS. 10.1021/bi9803933 Castanho, MARB; Prieto, MJE; Betbeder, D; Santos, NC; Pike, ER; Abbiss, JB. "The shape, dimension and organisation of maltodextrins gel fragments with and without associated phospholipids". Light Scattering and Photon Correlation Spectroscopy 40 (1997): 173-187. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000071347400013&KeyUID=WOS:000071347400013. 10.1007/978-94-011-5586-1_15 Loura, LMS; Prieto, M; Loura, L.M.S.; Prieto, M.. "Dehydroergosterol structural organization in a model system of membranes". Biophysical Journal 72 2 (1997): WPMC3-WPMC3. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1997WE74701371&KeyUID=WOS:A1997WE74701371. 10.1016/S0006-3495(97)78866-0 Santos, N.C.; Prieto, M.J.E.; Morna-Gomes, A.; Betbeder, D.; Castanho, M.A.R.B.; Santos, NC; Prieto, MJE; et al. "Structural characterization (shape and dimensions) and stability of polysaccharide/lipid nanoparticles". Biopolymers 41 5 (1997): 511-520. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031127551&partnerID=MN8TOARS. Published • 10.1002/(SICI)1097-0282(19970415)41:5<511::AID-BIP3>3.0.CO;2-P Santos, N.C.; Sousa, A.M.A.; Betbeder, D.; Prieto, M.; Castanho, M.A.R.B.. "Structural characterization of organized systems of polysaccharides and phospholipids by light scattering spectroscopy and electron microscopy". Carbohydrate Research 300 1 (1997): 31-40. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030988131&partnerID=MN8TOARS. 10.1016/S0008-6215(97)00025-6 Loura, LMS; Fedorov, A; Prieto, M; Loura, L.M.S.; Fedorov, A.; Prieto, M.. "Resonance energy transfer in a model system of membranes: Application to gel and liquid crystalline phases". Biophysical Journal 71 4 (1996): 1823-1836. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1996VK29500016&KeyUID=WOS:A1996VK29500016. 10.1016/S0006-3495(96)79383-9 Castanho, M.; Prieto, M.; Acuña, A.U.. "The transverse location of the fluorescent probe trans-parinaric acid in lipid bilayers". Biochimica et Biophysica Acta - Biomembranes 1279 2 (1996): 164-168. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029971806&partnerID=MN8TOARS. 10.1016/0005-2736(95)00251-0 Coutinho, A; Prieto, M; Coutinho, A.; Prieto, M.. "Self-association of the polyene antibiotic nystatin in dipalmitoylphosphatidylcholine vesicles: A time-resolved fluorescence study". Biophysical Journal 69 6 (1995): 2541-2557. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1995TV01800036&KeyUID=WOS:A1995TV01800036. 10.1016/S0006-3495(95)80125-6 CASTANHO, M; PRIETO, M. "FILIPIN FLUORESCENCE QUENCHING BY SPIN-LABELED PROBES - STUDIES IN AQUEOUS-SOLUTION AND IN A MEMBRANE MODEL SYSTEM". Biophysical Journal 69 1 (1995): 155-168. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1995RH66100016&KeyUID=WOS:A1995RH66100016. 10.1016/S0006-3495(95)79886-1 Prieto, M.J.E.; Castanho, M.; Coutinho, A.; Ortiz, A.; Aranda, F.J.; Gómez-Fernández, J.C.. "Fluorescence study of a derivatized diacylglycerol incorporated in model membranes". Chemistry and Physics of Lipids 69 1 (1994): 75-85. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028157976&partnerID=MN8TOARS. 10.1016/0009-3084(94)90029-9 CASTANHO, MARB; BROWN, W; PRIETO, MJE. "FILIPIN AND ITS INTERACTION WITH CHOLESTEROL IN AQUEOUS-MEDIA STUDIED USING STATIC AND DYNAMIC LIGHT-SCATTERING". Biopolymers 34 4 (1994): 447-456. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1994NE56300001&KeyUID=WOS:A1994NE56300001. 10.1002/bip.360340402 Castanho, M.A.R.B.; Brown, W.; Prieto, M.J.E.. "Filipin and its interaction with cholesterol in aqueous media studied using static and dynamic light scattering". Biopolymers 34 4 (1994): 447-456. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028419772&partnerID=MN8TOARS. COUTINHO, A; PRIETO, M. "RIBONUCLEASE-T(1) AND ALCOHOL-DEHYDROGENASE FLUORESCENCE QUENCHING BY ACRYLAMIDE - A LABORATORY EXPERIMENT FOR UNDERGRADUATE STUDENTS". Journal of Chemical Education 70 5 (1993): 425-428. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1993LD68200040&KeyUID=WOS:A1993LD68200040. 10.1021/ED070P425 MORENO, MJ; PRIETO, M. "INTERACTION OF THE PEPTIDE-HORMONE ADRENOCORTICOTROPIN, ACTH(1-24), WITH A MEMBRANE MODEL SYSTEM - A FLUORESCENCE STUDY". Photochemistry and Photobiology 57 3 (1993): 431-437. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1993KT69900006&KeyUID=WOS:A1993KT69900006. 10.1111/j.1751-1097.1993.tb02314.x Moreno, M.J.; Prieto, M.. "Interaction of the peptide hormone adrenocorticotropin, ACTH(1-24), with a membrane model system: a fluorescence study.". Photochemistry and Photobiology 57 3 (1993): 431-437. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027569610&partnerID=MN8TOARS. Coutinho, A.; Prieto, M.. "Ribonuclease T1 and alcohol dehydrogenase fluorescence quenching by acrylamide - A laboratory experiment for undergraduate students". Journal of Chemical Education 70 5 (1993): 425-428. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001336544&partnerID=MN8TOARS. Castanho, M.A.R.B.; Prieto, M.J.E.. "Fluorescence study of the macrolide pentaene antibiotic filipin in aqueous solution and in a model system of membranes". European Journal of Biochemistry 207 1 (1992): 125-134. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026720196&partnerID=MN8TOARS. 10.1111/j.1432-1033.1992.tb17029.x BERBERANSANTOS, MN; PRIETO, MJE; SZABO, AG. "PICOSECOND ELECTRONIC ENERGY-TRANSFER STUDIES IN SODIUM DODECYL-SULFATE MICELLES". Journal of the Chemical Society-Faraday Transactions 88 2 (1992): 255-261. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1992HC03100016&KeyUID=WOS:A1992HC03100016. 10.1039/ft9928800255 CASTANHO, MARB; BROWN, W; PRIETO, MJE. "ROD-LIKE CHOLESTEROL MICELLES IN AQUEOUS-SOLUTION STUDIED USING POLARIZED AND DEPOLARIZED DYNAMIC LIGHT-SCATTERING". Biophysical Journal 63 6 (1992): 1455-1461. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1992KF55100001&KeyUID=WOS:A1992KF55100001. 10.1016/S0006-3495(92)81733-2 CASTANHO, MARB; COUTINHO, A; PRIETO, MJE. "ABSORPTION AND FLUORESCENCE-SPECTRA OF POLYENE ANTIBIOTICS IN THE PRESENCE OF CHOLESTEROL". Journal of Biological Chemistry 267 1 (1992): 204-209. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1992GY43900037&KeyUID=WOS:A1992GY43900037. Castanho, M.A.R.B.; Coutinho, A.; Prieto, M.J.E.. "Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol". Journal of Biological Chemistry 267 1 (1992): 204-209. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026530622&partnerID=MN8TOARS. Villalain, J.; Prieto, M.. "Location and interaction of N-(9-anthroyloxy)-stearic acid probes incorporated in phosphatidylcholine vesicles". Chemistry and Physics of Lipids 59 1 (1991): 9-16. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025740410&partnerID=MN8TOARS. 10.1016/0009-3084(91)90058-J BERBERANSANTOS, MN; PRIETO, MJE; SZABO, AG. "EXCITED-STATE INTRAMOLECULAR RELAXATION OF THE LIPOPHILIC PROBE 12-(9-ANTHROYLOXY)STEARIC ACID". Journal of Physical Chemistry 95 14 (1991): 5471-5475. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1991FW47700023&KeyUID=WOS:A1991FW47700023. 10.1021/j100167a023 Berberan-Santos, M.N.; Prieto, M.J.E.; Szabo, A.G.. "Excited-state intramolecular relaxation of the lipophilic probe 12-(9-anthroyloxy)stearic acid". Journal of Physical Chemistry 95 14 (1991): 5471-5475. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037696346&partnerID=MN8TOARS. FARIA, JL; BERBERANSANTOS, M; PRIETO, MJE. "A COMMENT ON THE LOCALIZATION OF CYANINE DYE BINDING TO BRUSH-BORDER MEMBRANES BY THE FLUORESCENCE QUENCHING OF NORMAL-(9-ANTHROYLOXY) FATTY-ACID PROBES". Biochimica Et Biophysica Acta 1026 1 (1990): 133-134. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1990DT10200019&KeyUID=WOS:A1990DT10200019. 10.1016/0005-2736(90)90343-M COUTINHO, A; COSTA, J; FARIA, JL; BERBERANSANTOS, MN; PRIETO, MJE. "DIBUCAINE INTERACTION WITH PHOSPHOLIPID-VESICLES - A RESONANCE ENERGY-TRANSFER STUDY". European Journal of Biochemistry 189 2 (1990): 387-393. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1990DD27600023&KeyUID=WOS:A1990DD27600023. 10.1111/j.1432-1033.1990.tb15500.x Faria, J.L.; Berberan-Santos, M.; Prieto, M.J.E.. "A comment on the localization of cyanine dye binding to brush-border membranes by the fluorescence quenching of n-(9-anthroyloxy) fatty acid probes". Biochimica et Biophysica Acta - Biomembranes 1026 1 (1990): 133-134. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025299435&partnerID=MN8TOARS. Coutinho, A.; Costa, J.; Faria, J.L.; Berberan-Santos, M.N.; Prieto, M.J.E.. "Dibucaine interaction with phospholipid vesicles. A resonance energy-transfer study". European Journal of Biochemistry 189 2 (1990): 387-393. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025264182&partnerID=MN8TOARS. GOMEZFERNANDEZ, JC; VILLALAIN, J; ARANDA, FJ; ORTIZ, A; MICOL, V; COUTINHO, A; BERBERANSANTOS, MN; PRIETO, MJE. "LOCALIZATION OF ALPHA-TOCOPHEROL IN MEMBRANES". Annals of the New York Academy of Sciences 570 (1989): 109-120. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1989CV07100010&KeyUID=WOS:A1989CV07100010. 10.1111/j.1749-6632.1989.tb14912.x ARANDA, FJ; COUTINHO, A; BERBERANSANTOS, MN; PRIETO, MJE; GOMEZFERNANDEZ, JC. "FLUORESCENCE STUDY OF THE LOCATION AND DYNAMICS OF ALPHA-TOCOPHEROL IN PHOSPHOLIPID-VESICLES". Biochimica Et Biophysica Acta 985 1 (1989): 26-32. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1989AV81100004&KeyUID=WOS:A1989AV81100004. 10.1016/0005-2736(89)90098-9 Gomez-Fernandez, J.C.; Villalain, J.; Aranda, F.J.; Ortiz, A.; Micol, V.; Coutinho, A.; Berberan-Santos, M.N.; Prieto, M.J.E.. "Localization of a-tocopherol in membranes". Annals of the New York Academy of Sciences 570 (1989): 109-120. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024947916&partnerID=MN8TOARS. Aranda, F.J.; Coutinho, A.; Berberan-Santos, M.N.; Prieto, M.J.E.; Gómez-Fernández, J.C.. "Fluorescence study of the location and dynamics of a-tocopherol in phospholipid vesicles". BBA - Biomembranes 985 1 (1989): 26-32. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024421908&partnerID=MN8TOARS. BERBERANSANTOS, M; PRIETO, MJE. "MONTE-CARLO SIMULATION OF ORIENTATIONAL EFFECTS ON DIRECT ENERGY-TRANSFER". Journal of Chemical Physics 88 10 (1988): 6341-6349. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1988N555800036&KeyUID=WOS:A1988N555800036. 10.1063/1.454472 VILLALAIN, J; GOMEZFERNANDEZ, JC; PRIETO, MJE. "STRUCTURAL INFORMATION ON PROBE SOLUBILIZATION IN MICELLES BY FT-IR SPECTROSCOPY". Journal of Colloid and Interface Science 124 1 (1988): 233-237. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1988P165900026&KeyUID=WOS:A1988P165900026. 10.1016/0021-9797(88)90343-8 MELO, TSE; MACANITA, A; PRIETO, M; BAZIN, M; RONFARDHARET, JC; SANTUS, R. "PHOTOPHYSICAL BEHAVIOR OF 5-METHOXYPSORALEN IN DIOXANE-WATER MIXTURES". Photochemistry and Photobiology 48 4 (1988): 429-437. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1988Q515300005&KeyUID=WOS:A1988Q515300005. 10.1111/j.1751-1097.1988.tb02841.x Berberan-Santos, M.; Prieto, M.J.E.. "Monte Carlo simulation of orientational effects on direct energy transfer". The Journal of Chemical Physics 88 10 (1988): 6341-6349. http://www.scopus.com/inward/record.url?eid=2-s2.0-0039834292&partnerID=MN8TOARS. Sá e Melo, T.; Maçanita, A.; Prieto, M.; Bazin, M.; Ronfard-Haret, J.C.; Santus, R.. "Photophysical behaviour of 5-methoxypsoralen in dioxane-water mixtures.". Photochemistry and Photobiology 48 4 (1988): 429-437. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024100845&partnerID=MN8TOARS. Villalaín, J.; Gómez-Fernandez, J.C.; Prieto, M.J.E.. "Structural information on probe solubilization in micelles by FT-IR spectroscopy". Journal of Colloid And Interface Science 124 1 (1988): 233-237. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024050777&partnerID=MN8TOARS. BERBERANSANTOS, MN; PRIETO, MJE. "ENERGY-TRANSFER IN SPHERICAL GEOMETRY - APPLICATION TO MICELLES". Journal of the Chemical Society-Faraday Transactions Ii 83 (1987): 1391-1410. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1987J838000007&KeyUID=WOS:A1987J838000007. 10.1039/f29878301391 COSTA, SMD; PRIETO, MJ. "CHARGE AND ENERGY-TRANSFER PROCESSES IN EXCITED AMINOESTERS". Journal of Photochemistry 23 4 (1983): 343-354. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1983RV52700007&KeyUID=WOS:A1983RV52700007. De B. Costa, S.M.; Prieto, M.J.. "Charge and energy transfer processes in excited aminoesters". Journal of Photochemistry 23 4 (1983): 343-354. http://www.scopus.com/inward/record.url?eid=2-s2.0-0013121792&partnerID=MN8TOARS. 10.1016/0047-2670(83)87108-1 COSTA, SMB; PRIETO, MJ; GHIGGINO, KP; PHILLIPS, D; ROBERTS, AJ. "TIME-RESOLVED SPECTRA OF AN INTRA-MOLECULAR EXCIPLEX". Journal of Photochemistry 12 1 (1980): 11-16. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1980JC42500002&KeyUID=WOS:A1980JC42500002. COSTA, SMDB; PRIETO, MJ; DAVIDSON, RS. "INTRA-MOLECULAR EXCITED-STATE INTERACTIONS IN AMINO-ESTERS". Journal of Photochemistry 12 1 (1980): 1-10. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1980JC42500001&KeyUID=WOS:A1980JC42500001. de B. Costa, S.M.; Prieto, M.J.; Ghiggino, K.P.; Phillips, D.; Roberts, A.J.. "Time-resolved spectra of an intramolecular exciplex". Journal of Photochemistry 12 1 (1980): 11-16. http://www.scopus.com/inward/record.url?eid=2-s2.0-49149143324&partnerID=MN8TOARS. 10.1016/0047-2670(80)85075-1 de B. Costa, S.M.; Prieto, M.J.; Davidson, R.S.. "Intramolecular excited state interactions in amino-esters". Journal of Photochemistry 12 1 (1980): 1-10. http://www.scopus.com/inward/record.url?eid=2-s2.0-49149149488&partnerID=MN8TOARS. 10.1016/0047-2670(80)85074-X COSTA, SMDB; MACANITA, AL; MELO, ECC; PRIETO, MJ. "QUENCHING OF EXCITED AROMATIC ESTERS BY TRIETHYLAMINE". Journal of Photochemistry 11 5 (1979): 361-367. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1979HV98700006&KeyUID=WOS:A1979HV98700006. COSTA, SMDB; MACANITA, AL; PRIETO, MJ. "EXCITED-STATES OF AROMATIC ESTERS". Journal of Photochemistry 11 2 (1979): 109-119. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:A1979HJ54100005&KeyUID=WOS:A1979HJ54100005. de B. Costa, S.M.; Maçanita, A.L.; Prieto, M.J.. "Excited states of aromatic esters". Journal of Photochemistry 11 2 (1979): 109-119. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000330258&partnerID=MN8TOARS. 10.1016/0047-2670(79)80043-X de B. Costa, S.M.; Maçanita, A.L.; Melo, E.C.C.; Prieto, M.J.. "The quenching of excited aromatic esters by triethylamine". Journal of Photochemistry 11 5 (1979): 361-367. http://www.scopus.com/inward/record.url?eid=2-s2.0-49249151375&partnerID=MN8TOARS. 10.1016/0047-2670(79)85024-8
Thesis / Dissertation	Prieto, Manuel. "Interacções intramoleculares no estado excitado. Transferência de carga e transferência de energia". PhD, Universidade de Lisboa Instituto Superior Técnico, 1981.

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2020	Conformational Plasticity of the KcsA Channel from Advanced Time-Resolved Homo-FRET Methodologies	64th Annual Meetimng of the Biophysical Society Biophysical Society (US) (San Diego, United States)
2019	Insights into the mechanism of action of pepR, a viral-derived peptide, against Staphylococcus aureus biofilms	Gordon Research Conference on Antimicrobial Peptides Gordon Research Conferences (Lucca (Barga), Italy)
2019	Optimization of confocal microscopy imaging techniques to study the diffusion-controlled action of a cationic antimicrobial peptide along Staphylococcus biofilms	29th ECCMID ECCMID (Amsterdam, Netherlands)
2019	Remarkable Solar-Driven H2 Production from Polymeric Carbon Nitride Doped with Carbon and with Distorted Structure	XXVI Encontro Nacional da SPQ Sociedade Portuguesa de Química (Porto, Portugal)
2019	The hydrophobic pulmonary surfactant proteins SP-B and SP-C in interaction with membranes: topology, lipid selectivity and homo and hetero-oligomerization	48ª Reunião Anual da SBBq Sociedade Brasileira de Bioquímica e Biologia Molecular (Águas de Lindóia, Brazil)
2019	The effect of canonical and 1-deoxy(methyl) sphingoid bases in membrane fluidity and lateral organization	FEBS Special Meeting in Sphingolipid Biology: Sphingolipids in Physiology and Pathology FEBS (Cascais, Portugal)
2019	Fluorescence methodologies as key tools in Biology and Biophysics	Biophysics in the 21th Century European Biophysics' Societies Association (EBSA) (El Escorial, Spain)
2019	Membrane remodeling by the amyloidogenic peptide IAPP: surface crowing relevance	Joint 12th EBSA / 10th ICBP-IUPAP Biophysics Congress European Biophysics' Societies Association (EBSA) (Madrid, Spain)
2019	Fibers from proteins and lipids: Structure and dynamics from advanced spectroscopy and fluorescence microscopy methodologies (FCS, FRET, FLIM, and FRAP)	Visiting Fellow “Institute of Advanced Studies”, University of Warwick (England) (Warwick, United Kingdom)
2019	Lipid-protein supramolecular complexes	Vsiting Fellow Institut Charles Saudron, CNRS (Strasbourg, France)
2019	Hidratação e viscosidade da interface da membrana lipídica na presença de lípidos oxidados, e fibras amilóides de IAPP. Aplicação de metodologias avançadas de fluorescência resolvida no tempo	Simpósio FCT/FAPESP: “Oxidação Lipídica em Biofísica de Membranas e Celular: de nanosensores funcionais ao impacto sobre a formação de amilóides. Aplicação de técnicas avançadas de fluorescência, difusão de Raios-X e microscopias” Universidade de Sao Paulo (São Paulo, Brazil)
2018	Advanced Methodologies in Fluorescence and Fluorescence Microscopy	Advanced School: Protein-Protein Interaction and Protein-Membrane Interaction: experimental and theoretical approaches IUBMB/IUPAB (Havana, Cuba)
2018	About Biophysics	1st Biophysics Party Portuguese Biophysical Society (Lisbon, Portugal)
2018	g-c3n4/zn2al-ldh 2d/2d heterojunction for light-induced photcatalytic hydrogen evolution	VIth Jornadas Ibéricas de Fotoquímica Portuguese and Spanish Chemical Societies (Aveiro, Portugal)
2018	The membrane potassium channel KcsA - Exact analytical solutions for advanced homo-FRET to retrieve structural information	VIth Jornadas Ibéricas de Fotoquímica Portuguese Chemical Society (Aveiro, Portugal)
2018	Zn(ii)-meso_-tetrakis(_meta_-carboxyphenyl)porphyrin and g-c3n4: Efficient light-driven hydrogen generation from water without addition of co-catalyst	7th EuCheMS Chemistry Congress European Chemical Societies (Liverpool, United Kingdom)
2018	Surface crowding and membrane remodeling by IAPP	XLIII Congresso da Sociedade Brasileira de Biofísica Brazilian Biophysics Society (Santos, Brazil)
2018	The membrane potassium channel KcsA: Exact analytical solutions for advanced homo-FRET to retrieve structural information	7th Wroclaw-Prague Seminar on Biophysics of Lipids (Wroclaw, Poland)
2018	Modified g-C3N4 with Improved Enhanced Charge Separation for Efficient Visible-light Photocatalytic H2 Production	10th European meeting on Solar Chemistry and Photocatalysis: Environmental Applications (SPEA10) (Almería, Spain)
2018	Studying the antibiofilm properties of the peptide pepR using a biophysical approach	Biofilms 8 (Aarhus, Denmark)
2018	Unravelling the activity and mechanism of action of pepR, a viral-derived membrane-active peptide, against Staphylococcus aureus biofilms	6th International Iberian Biophysics Congress and X Ibero-american Congress of Biophysics Portuguese and Spanish Biophysical Societies (Castellón, Spain)
2018	Moléculas llenas de energía: Luz y colores en la naturaleza	Congreso de Enseñanza de Ciencias Básicas Universidad de la Republica Uruguaya (Salto, Uruguay)
2018	Principles of Fluorescence and Energy Transfer (FRET) Methodologies	Training School on the basics of Photochemical, Photophysical and Photobiological Processes 6th Jornadas Ibéricas de Fotoquímica (Aveiro, Portugal)
2017	Biophysics: a quantitative approach to life sciences	Biophysics Week Universidad Complutense (Madrid, Spain)
2017	Membrane biophysics, phases and lipid domains. Cholesterol in membranes.	Biophysics in the 21st Century European Biophysics' Societies Association (EBSA) (Edinburgh, United Kingdom)
2017	Nanoscale Compartmentalization of PI(4,5)P2 in Living Cells and Model Membranes. A Fluorescence Spectroscopy and FRET Imaging study	XXV National SPQ Meeting Sociedade Portuguesa de Química (Lisbon, Portugal)
2017	Proteins on membrane interfaces: Structure and dynamics of lipid-protein fibers from advanced FRET methodologies and microscopy	. VII Iberian Meeting on Colloids and Interfaces (RICI-VII) Universidad Complutense (Madrid, Spain)
2017	Advanced homo-FRET – Exact analytical solutions for tetramers and their application for retrieving structural information of the KcsA channel	10th International Weber Symposium on Fluorescence Methodologies in Biochemistry and Medicine Sociedade Brasileira Biofísica (Búzios, Brazil)
2017	Modulation of Bax-induced membrane permeabilization by cytoprotective bile acids: A Biophysical study.	Congresso da Sociedade Brasileira de Biofísica Sociedade Brasileira Biofísica (São Paulo, Brazil)
2017	Fibers from proteins and lipids: Structure and dynamics from advanced spectroscopy and fluorescence microscopy methodologies	XXXIII Encontro Galego-Portugues de Quimica Portuguese and Spanish Chemical Societies (El Ferrol, Spain)
2017	Molecules full of energy: Light and colors in nature	Congreso Internacional de Enseñanza de las Ciencias Basicas UNESCO (Salto, Uruguay)
2017	Advanced FRET modelling in biophysics – Exact analytical solutions on structural studies of the KcsA channel and FRET in 2D under the microscope	11th International Symposium on Cell Surface Macromolecules (Chandigarh, India)
2016	Nanoparticle and lipid nanodomain insertion in the membrane of large and giant copolymer vesicles	6th Somas School Synthetic and Biological Membranes, (Mittelwihr, France)
2016	Quantification of nanoscale compartmentalization in the plasma membrane of living cells through FRET imaging	II Congresso Regional da Sociedade Brasileira de Biofísica Sociedade Brasileira Biofísica (Aracaju, Brazil)
2016	Analytical solutions for advanced ensemble average FRET in biophysics	III Latin American Federation of Biophysical Societies/ IX Ibero American Congress of Biophysics Latin American Federation of Biophysical Societies (Tucumán, Argentina)
2016	Analysis of nanoscale compartmentalization of PI(4,5)P2 in the plasma membrane through FRET imaging	Spanish-Portuguese Meeting for Advanced Optical Microscopy (SPAOM) (Bilbao, Spain)
2016	Analysis of nanoscale compartmentalization of PI(4,5)P2 in living cells and model membranes through fluorescence spectroscopy and FRET imaging	5 th International Iberian Biophysics Congress Portuguese and Spanish Biophysical Societies (Porto, Portugal)
2016	Ceramide-gel domains in living cells: Understanding the role of embalmer biophysical properties in cell (patho)physiology	5 th International Iberian Biophysics Congress Portuguese and Spanish Biophysical Societies (Porto, Portugal)
2015	Quantifying the membrane assembly of amphitropic proteins by homo-FRET analysis	Biophysics of proteins and surfaces: Assembly, Activation, Signaling (Madrid, Spain)
2015	FRET analysis of the nanoscale organization of PI(4,5)P2 in living cells	Biophysics of proteins and surfaces: Assembly, Activation, Signaling Spanish and USA Biophysical Societies (Madrid, Spain)
2015	Amyloid-like fibers and the role of lipids: Structure and dynamics from advanced FRET methodologies and microscopy	Latin American Crosstalk in Biophysics Latin American Federation of Biophysical Societies (Salto, Uruguay)
2015	Lipid-Protein interaction from advanced fluorescence and microscopy methodologies: Topology, dynamics and lipid selectivity	VIII Poslatam Course: Membrane Lipids, Tranposters, Channels and all that Crosstalk Latin American Federation of Biophysical Societies (Salto, Uruguay)
2015	Lipid-protein supramolecular complexes: structure and dynamics of “amyloid- like” fibers	WE-Heraeus Seminar: Avanced microscopy of membrane biophysics (Bad Honnef, Germany)
2015	Ceramide-Induced Biophysical Alterations in Membranes of Live Cells	International Ceramide Conference/Sphingolipid Club joint meeting (Izmir (Cesme), Turkey)
2015	Unravelling the Biophysical Properties of Sphingolipids: From Model to Cell Membranes	International Ceramide Conference/Sphingolipid Club joint meeting (Izmir (Cesme), Turkey)
2015	Interaction of cytotoxic and cytoprotective bile acids with lipid membranes: Merging molecular and cell biophysics	I Congresso Regional da Sociedade Brasileira de Biofísica Sociedade Brasileira Biofísica (Natal, Brazil)
2015	Analysis of PI(4,5)P2 lateral organization at the plasma membrane of living cells through FRET	59th meeting of the Biophysical Society Biophysical Society (US) (Baltimore, United States)
2014	Fluorescence techniques and methodologies in membrane biophysics: Structural and dynamic information	XXXIX Congresso da Sociedade Brasileira de Biofísica Sociedade Brasileira Biofísica (Caxambu, Brazil)
2014	Modulators of PI(4,5)P2 Lateral Distribution in Model Systems and Cellular Membranes	Cellular imaging of Lipids - EMBO Workshop EMBO (Vico Equense, Italy)
2014	Mechanisms of action of cytotoxic and cytoprotective bile acids – shaping membrane structure or permeability?	XIV Congress of the Spanish Biophysical Society Spanish Biophysical Society (Alcalá de Henares, Spain)
2014	Impact of Ca2+ on PI(4,5)P2 interactions with PI(4,5)P2 binding proteins	Membrane, Morphology and Function -FEBS Meeting FEBS (Abruzzo)
2014	Phase separation within Hybrid Polymer/Lipid Vesicles used as Biomimetic Membranes	The Physics of Soft and Biological Matter IOP Conference IOP (London, United Kingdom)
2014	Detection and characterization of calcium induced PI(4,5)P2 clustering in membranes. Application of advanced fluorescence and microscopy methodologies	43ª Annual Meeting SBBQ Sociedade Brasileira de Bioquímica (Foz de Iguaçu, Brazil)
2014	Physiological Ca2+ concentrations induce PI(4,5)P2 clustering and have an impact in PI(4,5)P2 partition properties	4th Young Portuguese Chemists Meeting Portuguese Chemical Society
2014	Tackling sphingolipids’ biophysical properties to decipher their biological roles	Symposium Sphingolipids in Health and Disease (Porto, Portugal)
2014	Characterization of ceramide-induced changes on the biophysical properties of cellular membranes	2nd International Workshop on Molecular Medicine of Sphingolipids (Kloster Banz, Germany)
2014	Ceramide-platforms@the endolysosomal pathway: a novel mechanism by which ceramide activates intracellular targets?	International Conference on the Bioscience of Lipids (Aberdeen, United Kingdom)
2014	Ceramide-platforms@the endo/lysosomal pathway: a novel mechanism by which ceramide activates intracellular targets?	Gordon Research Conference on Glycolipid and Sphingolipid Biology Gordon Research Conferences (Ventura, United States)
2014	Imaging Ceramide platforms in model and cell membranes	EMBO Workshop on “Lipid Imaging EMBO (Naples, Italy)
2014	Application of advanced fluorescence and microscopy approaches to the study of PI(4,5)P2 clustering in membranes	Xth International Symposium on Cell Surface Macromolecules (Calcutta, India)
2014	Characterization of PI(4,5)P2 distribution in membranes through fluorescence spectroscopy and FRET microscopy	1st International Caparica Conference on Chromogenic and Emissive Materials Universidade Nova de Lisboa (Costa da Caparica, Portugal)
2014	Structure and dynamics of amyloid-like protein fibers: FCS, FRET, FLIM, and FRAP	XIV Iberian Peptide Meeting (Bilbao, Spain)
2014	Insights into the biophysics underlying lipid interactions in nanoliposomes: clues from fluorescence spectroscopy, confocal and two-photon microscopy	1st International Symposium on Nanoparticles/Nanomaterials and Applications (Costa da Caparica, Portugal)
2013	PI(4,5)P2 acts as a lipid calcium sensor in the presence of physiological calcium concentrations	9th European Biophysics Congress European Biophysics' Societies Association (EBSA) (Lisbon, Portugal)
2013	Size and dynamics of PI(4,5)P2 clusters, induced by calcium concentrations in the physiological range in model systems of membranes: Application of advanced fluorescence and microscopy approaches	VIII Ibero-American Congress of Biophysics (Valparaíso, Chile)
2013	Physiological concentrations of calcium induce PI(4,5)P2 clustering in membranes: Application of advanced fluorescence and microscopy approaches	25th Annual Meeting of the Turkish Biophysical Society Turkish Biophysical Society (Trabzon, Turkey)
2013	Glucosylceramide as a signaling lipid: link between unbalanced metabolism, membrane properties and development of Gaucher Disease	7th International Ceramide Conference (Montauk, New York, United States)
2013	Ceramide C24:0 sharply changes membrane organization and morphology	5th Post-graduate iMed.UL Students Meeting Faculty of Pharmacy, Lisbon University
2013	Amyloid-like fibers formed upon interaction of proteins and anionic lipids: Structure and dynamics from advanced FRET methodologies and microscopy	Physics Meets Biology at the Cell Membrane” The Iberian Membrane Biophysics Colloquium (San Sebastian, Spain)
2013	Ceramide and Glucosylceramide impact on membrane biophysical properties: from model to cell membranes	XLII Annual Meeting Argentinian Biophysical Society Argentinian Biophysical Society (Cordoba, Argentina)
2013	Ceramide C24:0 sharply changes membrane organization and morphology	5th Post-graduate iMed.UL Students Meeting Faculdade de Farmácia, Universidade de Lisboa (Lisbon, Portugal)
2013	Ceramide activates endocytosis and forms ordered intracellular lipid domains in response to TNF-alpha	9th European Biophysics Congress EBSA (Lisbon, Portugal)
2013	Physiological calcium concentrations induce PI(4,5)P2 clustering: PI(4,5)P2 as a lipidic calcium sensor	Biophysical Society 57th Annual Meeting Biophysical Society USA (San Diego, United States)
2013	Structure and dynamics of “amyloid-like” lipid-protein fibers: a FCS, FRET, FLIM and FRAP study	Spring 2013 American Chemical Society National Meeting ACS (New Orleans, United States)
2012	Tethering of lipid membranes reorganizes the distribution of lipid domains	XXXV BM) Spanish Society for Biochemistry and Molecular Biology (SEBBM) Congress Spanish Society for Biochemistry and Molecular Biology (SEBBM) (Seville, Spain)
2012	Amyloid-like fiber formation driven by lipid-protein interaction: Structure and dynamics from homo-FRET, FLIM and FCS	Lipid-protein interaction in membranes CCMB Hyderabad (Hyderabad, India)
2012	Membrane biophysics and lipid-protein interaction	2nd EBSA Course on Membranes EBSA (Arcachon, France)
2012	Membranes domains, lipid phase diagrams, rafts and ceramides: Advanced fluorescence methodologies in membrane biophysics	Strasbourg Workshop on Membrane Biophysics CNRS Strasbourg (Strasbourg, France)
2012	Coupled lysozyme partition/oligomerization triggered by anionic lipid vesicles: a time-resolved study	XIII Iberian Peptide Meeting (Alicante, Spain)
2012	Exploring fluorescence lifetime and homo-FRET measurements to monitor lysozyme oligomerization in anionic lipid membranes: relation to “amyloid-like” fibril formation	Biophysical Society 56th Annual Meeting Biophysical Society USA (San Diego, United States)
2012	Fluorescence and microscopy in membrane biophysics: rafts, ceramides, amyloids, and lipid-protein interaction	XV International Symposium on Luminescence Spectrometry, ISLS (Barcelona, Spain)
2012	Ceramide platforms and microdomains function	Gordon Research Conference on Glycolipid and Sphingolipid Biology Gordon Research Conferences (Il Ciocco in Lucca, Italy)
2011	Gel and fluid membrane domains in interaction with proteins: Membrane morphological alterations and amyloid-like fiber formation	Congreso XXV Aniversario de la SBE Sociedad Biofísca España (Murcia, Spain)
2011	Advanced FRET methodologies in membrane biophysics	III Jornadas Ibéricas de Fotoquímica Portuguese and Spanish Chemical Societies (Granada, Spain)
2011	Advanced FRET methodologies: Lipid selectivity and membrane protein-drug interaction	9th International Symposium on Biochemical Roles of Eukaryotic Cell Surface Macromolecules (Trivandrum, India)
2010	Lysozyme binding to phosphatidylserine-containing membranes: implications in amyloid-like fiber formation	XII Iberian Peptide Meeting (Lisbon, Portugal)
2010	Amyloid-like fiber formation: the role of lipid-protein interaction	4th ISN Special Conference on Membrane Domains in CNS Physiology and Pathology ISN (Erice, Italy)
2010	Lipid Raft Composition Modulates Sphingomyelinase Activity and Ceramide-Induced Membrane Physical Alterations	54 th Annual Meeting of the Biophysical Society Biophysical Society (US) (San Francisco, United States)
2010	Rafts and ceramides: biophysical perspective of biologically relevant membrane domains	51st International Conference of the Bioscience of Lipids (ICBL) (Bilbao, Spain)
2010	Lipid-protein interaction related to amyloid-like fiber formation	Survival or Death as a Matter of Fat (Coimbra, Portugal)
2009	Amyloid-like fiber formation driven by lipid-protein interaction	XXXVIII Reunião Anual da Sociedade Brasileira de Bioquímica e Biologia Molecular Sociedade Brasileira de Bioquímica e Biologia Molecular (Águas de Lindóia, Brazil)
2009	Rafts, ceramides, and fluid lipids: Phase diagrams determination from photophysical methodologies	International Conference of Fluorescence in Biology (Mumbai, India)
2009	Sphingomyelinase activity and ceramide-induced membrane physical alterations are strongly dependent on lipid raft composition	5th International Charleston Ceramide Conference (Charleston, USA, United States)
2009	Effects of pulmonary surfactant protein SP-C on liquid-ordered/liquid-disordered phase coe-existence in a ternary membrane model system	Final General Assembly-BIOMEM EU-COST (Rovinji, Croatia)
2009	RBP-receptor structure and funcion	Final General Assembly-BIOMEM COST-EU (Rovinji, Croatia)
2008	Biophysics of rafts and their interplay with ceramides	49th International Conference on the Bioscience of Lipids (Maastricht, Netherlands)
2008	Glucosylceramide and Ceramide induce similar alterations in membrane biophysical properties	Gordon Research Conference on Glycolipid and Sphingolipid Biology Gordon Research Conferences (Il Ciocco in Lucca, Italy)
2008	Ceramide, sphingomyelin and unsaturated lipids in the absence of cholesterol: Phase diagrams, gel phases and their biological relevance	1st Portuguese-Spanish-British Joint Biophysical Congress Portuguese, Spanish, British Biophysical Societies (Lisbon, Portugal)
2008	Nanostructure of protein amyloid-like fibers induced by membranes	The 8th International Symposium on Biochemical Roles o Eukaryotic Cell Surface Macromolecules (Hyderabad, India)
2008	Time-resolved resonance energy transfer in membrane biophysics	XXI Reunión nacional de Espectroscopia (Murcia, Spain)
2008	Lipid domains and lipid-protein interaction: Physical-chemistry and spectroscopy	XXI Encontro Nacional da Sociedade Portuguesa de Química Sociedade Portuguesa de Química (Porto, Portugal)
2008	Lipid raft composition modulates sphingomyelinase activity and ceramide-induced membrane physical alterations	FEBS Workshop- Lipids as regulators of cell function FEBS (Spetses, Greece)
2007	Modulation of lateral membrane organization by the interplay of ceramide with lipid rafts	6th European Biophysics Congress European Biophysics' Societies Association (EBSA) (London, United Kingdom)
2007	Peptide/protein-induced structural alterations in membranes	2 nd Worshop on the Biophysics of Membrane-active peptides (Lisbon, Portugal)
2007	Structural characterization of pulmonary surfactant SP-B in model membranes by fluorescence spectroscopy	48 th International Conference on the Bioscience of Lipids (ICBL) ICBL (Turku, Finland)
2007	Ceramide recruits 3 sphingomyelin molecules to form nanometer-sized gel domains that are destroyed by cholesterol in lipid rafts	51th Annual Meeting of the Biophysical Society Biophysical Society (US) (Baltimore, United States)
2007	Complexity of Lipid Domains and Rafts in Giant Unilamellar Vesicles Revealed by Combining Imaging, Microscopic and Macroscopic Time-resolved Fluorescence	51th Annual Meeeting of the Biophysical Society Biophysical Society (US) (Baltimore, United States)
2007	Fluorescence: Principles and Methodologies	II Curso Ibérico de Fotoquímica Portuguese and Spanish Chemical Societies (Faro, Portugal)
2006	Sphingomyelin/cholesterol rafts and ceramide platforms: Biophysical perspectives	Symposium: To Raft or not to Raft, that is the question (Leuven, Belgium)
2006	Interaction of Two Cecropin-Melittin Antimicrobial Peptides with Model Membranes.Calorimetric, Time Resolved Fluorescence Spectroscopy and Linear Dichroism results	19 th International Conference on Chemical Thermodynamics (Bpulder, Colorado, United States)
2006	Sphingomyelin/cholesterol rafts, ceramide platforms, PIP2 domains and basic peptides in interaction with membranes: Biophysical perspectives	Final COST Report COST-EU (Murcia, Spain)
2006	Membrane Proteins: Structural studies from fluorescence methodologies	First Assembly of the Marie Curie EST Program in Membrane Biology Marie Curie EST Program ( Utrecht, Netherlands)
2006	Lipid domains, rafts and lipid-protein interaction: Biophysical perspectives from fluorescence methodologies	Meeting of the Members of the IUPAB Council IUPAB (Ottawa, Canada)
2006	Interaction of S413-PV cell penetrating peptide with model membranes: Relevance to peptide translocation across biological membranes	VI Ibero-American Congress of Biophysics (Madrid, Spain)
2006	Ceramide-domain formation and collapse in lipid rafts: membrane reorganization by an apoptotic lipid. Comparison to ganglioside GM1	2nd ISN special Neurochemistry conference: Neural glycoproteins and glycolipids ISN (Antigua, Antigua and Barbuda)
2005	Fluorescence methodologies in membrane biophysics. Lipid rafts and lipid-protein interaction	9 th International Conference on Methods and Applications of Fluorescence. Spectroscopy, Imaging and Probes (Lisbon, Portugal)
2005	Biophysical properties of ceramide-containing lipid bilayers using fluorescence methodologies	Workshop COST D22: Membrane Domains and Lipid Asymmetry COST-EU
2005	Photophysical techniques applied to the detection of the binding site of V-ATPase inhibitors	8º Encontro Nacional de Fotoquímica Sociedade Portuguesa de Química (Coimbra, Portugal)
2005	Resonance energy transfer and topologies in membrane biophysics	I Jornadas do Centro de Química da Universidade do Minho Centro de Química da Universidade do Minho (Guimarães, Portugal)
2005	Fluorescence resonance energy transfer and domain formation in membranes	VII Congreso de Fotoquímica da Real Sociedad Española de Química Real Sociedad Española de Química (Logroño, Spain)
2005	Membrane biophysics, lipid rafts and lipid-protein interaction	Biophysical chemistry meets molecular medicine” CERC 3 (Sesimbra, Portugal)
2005	Binding of V-ATPase inhibitors towards the 4th transmembrane segment of the enzyme’s subunit c	COST D22 Working Group Meeting on Lipid-Protein Interactions COST-EU (Cosenza, Italy)
2005	Ternary phase diagrams and lipid domains	COST Action D22: Membrane domains and lipid asymmetry COST-EU (Utrecht, Netherlands)
2005	The organization of the gamma M4 transmembrane domain of the muscle nicotinic acetylcholine receptor in cholesterol containing membranes	COST Action D22: Principles of membrane protein folding and stability COST-EU (Lisbon, Portugal)
2004	Lipid rafts and lipid-protein interaction. Fluorescence methodologies in membrane biophysics	3rd Portuguese - Spanish Biophysics Congress Portuguese and Spanish Biophysical Societies (Lisbon, Portugal)
2004	Fluorescence spectroscopy of membrane protein assembly	1st Domain WG meeting- COST Action D22 Working Group Meeting COST-EU (Szeged, Hungary)
2004	Lipid rafts have different sizes depending on the membrane composition	XIV Congresso Nacional de Bioquímica Sociedade Portuguesa de Bioquímica (Vilamoura, Portugal)
2004	Phase diagrams and raft sizes as determined by fluorescence methods	Mid – Term Review Meeting COST Action D:22, Lipid – Protein interaction COST-EU (Madrid, Spain)
2003	Photophysical approaches to the structure and dynamics of lipid microdomains in model membrane systems	7 th International Conference on Solar Energy and Applied Photochemistry”, and “4th International Training Workshop on Environmental Photochemistry (Luxor, Egypt)
2003	Peptide interaction with two-component membranes. Domain formation or vesicle aggregation	4 th European Biophysics Congress European Biophysics' Societies Association (EBSA) (Alicante, Spain)
2003	A ternary phase diagram for rafts model system	Microdomains, lipid rafts and caveolae; ESF/EMBO Conference ESF/EMBO (Tomar, Portugal)
2003	Fluorescence in Biochemistry and Biophysics	I Curso Ibérico de Fotoquímica Sociedades Portuguesa e Espanhola de Química (Santiago de Compostela, Spain)
2003	A model for raft / non-raft coexistence. Phosphatidylcholine / sphingomyelin / cholesterol ternary phase diagram	47 th Annual Meeting of the Biophysical Society Biophysical Society (US) (San Antonio, Texas, United States)
2002	Peptide interaction with two-component lipidic systems. Application of photophysical methodologies	Structure and dynamics of lipid interfaces”- Satellite Meeting of the “XIV International Biophysics Congress" IUPAB (Buenos Aires, Argentina)
2002	Phase separation in biomembranes - proteins and rafts: studies in model systems	XIII Congresso Nacional de Bioquímica Sociedade Portuguesa de Bioquímica (Lisboa, Portugal)
2002	Structure and dynamics of acetylcholine receptor and its lipid microenvironment: from molecule to cell	XI th International Symposium on cholinergic mechanisms (St. Moritz, Switzerland)
2002	The role of sterols in the mechanism of aggregate formation by the polyene antibiotic nystatin	1st Domain WG meeting- “Membrane domains and rafts”-COST Action D22: Protein-Lipid Interaction COST-EU (Segovia, Spain)
2002	Phase diagrams of rafts model systems	1st Domain WG meeting- “Membrane domains and rafts”-COST Action D22: Protein-Lipid Interaction COST-EU (Segovia, Spain)
2002	Application of optical spectroscopic techniques (UV-Vis absorption, ORD and CD, steady-state and time-resolved fluorescence, fluorescence anisotropy) to biochemical and macromolecular systems	Lipids and Proteins: Structural aspects Argentinian Biophysical Society (Buenos Aires, Argentina)
2002	M13 major coat protein aggregation in model systems of membranes. FRET methodology and preliminary data	Molecular interactions of the lipid–protein interface (Calabria, Italy)
2001	Detection and characterization of membrane microheterogeneity by resonance energy transfer	Final workshop of the bilateral scientific and technological cooperation between Flanders and Poland (Leuven, Belgium)
2001	Photophysical approaches to the structure and dynamics of lipid microdomains in model membrane systems	Workshop on Fluorescence Techniques in Biophysics (Lyngby, Denmark)
2001	Lipid microdomains in membranes probed by fluorescence spectroscopy	Lipid-protein supramolecular assemblies (Zagreb, Croatia)
2000	Structure and dynamics of lipidic domains in model membrane systems	IV Congreso Iberoamericano de Biofísica (Alicante, Spain)
1999	Heterogeneidades em sistemas modelo de membranas biológicas. Aplicação de técnicas de fluorescência	Encontro Luso-Galego de Química (Vigo, Spain)
1999	Interaction of the major epitope region of HIV protein gp41 with membrane model systems	4th International Weber Symposium in Innovative Methodologies in Biochemistry and Medicine (Kaanapali, Maui, United States)
1999	Lipid microdomains in membrane model systems probed by fluorescence energy transfer	Symposium on Membranes, Sensors and Cell Surfaces (Hyderabad, India)
1999	Distribution heterogeneity in membrane model systems probed by fluorescence resonance energy transfer	4th International Weber Symposium in Innovative Methodologies in Biochemistry and Medicine (Kaanapali, Maui, United States)
1998	Interaction of the major epitope region of HIV protein gp41 with membrane model systems	2nd Portuguese-Spanish Biophysics Congress / VI Congreso de la Sociedad de Biofísica de España Portuguese and Spanish Biophysical Societies (Madrid, Spain)
1998	Strong and very weak coupling limits in electric dipolar interactions. Applications in structural biochemistry	2nd International Biophysics Conference (Cairo, Egypt)
1998	Distribution heterogeneity in two-phase, two-component lipid mixtures probed by resonance energy transfer	2nd Portuguese-Spanish Biophysics Congress / VI Congreso de la Sociedad de Biofísica de España Portuguese and Spanish Biophysical Societies (Madrid, Spain)
1998	Dipolar interactions in systems of restricted dimensionality. Application to membrane model systems and micelles	Reunión Bianual de la Real Sociedad Española de Química Spanish Chemical Society (Cádiz, Spain)
1997	Dehydroergosterol structural organization in a model system of membranes	41st Annual Meeting of the Biophysical Society Biophysical Society (US) (New Orleans, United States)
1996	Organização de esteróis em sistemas modelo de membranas biológicas	X Congresso Nacional de Bioquímica Sociedade Portuguesa de Bioquímica (Braga, Portugal)
1995	Energy transfer in restricted dimensionality. Application to a model system of membranes	1st Portuguese-Spanish Biophysics Congress-V Congreso de la Sociedad de Biofísica de Espanha Portuguese and Spanish Biophysical Societies (Lisbon, Portugal)
1995	A new drug carrier system made of dextran gel fragments and phospholipids. Caracterization by light scattering	1st International Meeting of the Portuguese Carbohydrate Chemistry Group Sociedade Portuguesa de Química (Lisbon, Portugal)
1994	Electronic dipolar interactions: Formalisms, methodologies and its application to membranes	IV Congreso de la Sociedad de Biofísica de España Spanish Biophysical Society (Cáceres, Spain)
1993	Static and dynamic light scattering studies of filipin in aqueous media and its interaction with cholesterol	37th Annual Meeting of the Biophysical Society Biophysical Society (US) (Washington, United States)
1993	Localização transversal de compostos em sistemas modelo de membranas	9º Congresso Nacional de Bioquímica Sociedade Portuguesa de Bioquímica (Albufeira, Portugal)
1985	Informação estrutural sobre agregados moleculares a partir de técnicas espectroscópicas	8º Encontro Anual da Sociedade Portuguesa de Química Sociedade Portuguesa de Química (Braga, Portugal)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2018 - 2021	The role of PI(4,5)P2 acyl-chains as regulators of PI(4,5)P2/HIV-1 Gag clustering in model membranes Co-supervisor of Luís Pedro Borges Araújo	Universidade de Lisboa Instituto Superior Técnico, Portugal
2018 - 2021	Structural insights into the molecular basis of lipid-dependent gating of KcsA, a model potassium channel Co-supervisor of Clara Díaz García	Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 - 2020	Implication of stress stimuli-derived ceramides on cell membrane biophysical properties: understanding the molecular mechanisms of ceramides action Co-supervisor of Ana Ester Limede e Ventura	Universidade de Lisboa Faculdade de Farmácia, Portugal
2015 - 2020	Biological and biophysical properties of atypical sphingolipids: implications to physiology and pathophysiology Co-supervisor of Tânia da Cunha Branco dos Santos	Universidade de Lisboa Faculdade de Farmácia, Portugal
2016 - 2019	Probing the interplay between calcitonins and islet amyloid polypeptides with membranes using advanced fluorescence methodologies Co-supervisor of Joana Catarino Ribeiro Ricardo	Universidade de Lisboa Instituto Superior Técnico, Portugal
2015 - 2018	Molecular mechanisms of bile-acid induced apoptosis and cytoprotection Co-supervisor of Tânia Marques de Patrícia Sousa	Universidade de Lisboa Instituto Superior Técnico, Portugal
2013 - 2016	Lateral organization of PI(4,5)P2 in model membranes and at the plasma membrane of living cells Co-supervisor of Maria João Martins Sarmento	Universidade de Lisboa Instituto Superior Técnico, Portugal
2013 - 2016	Hybrid Polymer/Lipid Vesicles as New Particles for Drug-Delivery and Cell Mimics Co-supervisor of Dao Thi Phuong Tuyen	Université de Bordeaux, France
2012 - 2015	The link between altered glucosylceramide composition and membrane properties: From membrane biophysics to Gaucher Diseases Co-supervisor of Ana Raquel Pinto Varela	Universidade de Lisboa Faculdade de Farmácia, Portugal
2011 - 2014	Lipid-protein interaction in amyloid-like fiber formation Supervisor of Ana Margarida Pereira de Melo	Universidade de Lisboa Instituto Superior Técnico, Portugal
2010 - 2013	Finding the biophysical mechanisms underlying the distinct biological action of ceramide Supervisor of Sandra Cristina Nunes Trigo Pinto	Universidade de Lisboa Instituto Superior Técnico, Portugal
2009 - 2012	Ceramide and alkyl ether lipids interactions with lipid rafts: effects on membrane physical properties, cell death receptor organization and apoptosis induction Supervisor of Bruno Miguel Mota Castro	Universidade de Lisboa Instituto Superior Técnico, Portugal
2008 - 2011	Lipocalin receptor structure/function Supervisor of Mari Cármen López Ruiz	Universidade de Lisboa Instituto Superior Técnico, Portugal
2007 - 2010	Lateral sorting of proteins into lipid rafts and protein-protein interactions as prerequisite for assembly of influenza virus Co-supervisor of Florian Baumgart	Universidad Complutense de Madrid, Spain
2006 - 2009	Lateral organization of the transmembrane domain and cytoplasmic tail of influenza virus hemagglutinin revealed by time-resolved imaging Co-supervisor of Silvia Scolari	Humboldt-Universität zu Berlin, Germany
2004 - 2007	Estudo de proteínas membranares em sistemas modelo de biomembranas. Caracterização das interacções proteína-proteína e proteína-lípido por técnicas de fluorescência Supervisor of Fábio Monteiro Fernandes	Universidade de Lisboa Instituto Superior Técnico, Portugal
2003 - 2006	Obtenção de parâmetros estruturais de orientação e distribuição lateral de sondas de membrana e moléculas clinicamente relevantes inseridas em sistemas modelo de membranas biológicas Supervisor of Sílvia do Carmo Dias Neves Lopes	Universidade de Lisboa Instituto Superior Técnico, Portugal
2003 - 2006	Nystatin and ceramide interaction with multicomponent model membranes: relating membrane biophysics with antibiotic action and apoptosis Supervisor of Liana Casquinha da Silva	Universidade de Lisboa Instituto Superior Técnico, Portugal
2002 - 2005	Structural and physical-chemical characterization of lipoplexes for gene therapy and DNA vaccines Co-supervisor of Teresa Catarina Páscoa Madeira	Universidade de Lisboa Instituto Superior Técnico, Portugal
2001 - 2004	Estudos biofísicos em bicamadas lipídicas: separação de fases e interacção lípido-proteína Supervisor of Rodrigo Freire Martins de Almeida	Universidade de Lisboa Instituto Superior Técnico, Portugal
1997 - 2000	Kinetics of bimolecular reactions with stochastic effects and dimensional constraints Co-supervisor of Maria João Pedrosa Ferreira Moreno Silvestre	Universidade Nova de Lisboa, Portugal
1997 - 2000	A determinação de propriedades estruturais, conformacionais e dinâmicas de moléculas em sistemas modelo de membranas e em solução. Estudos de simulação por Dinâmica Browniana Co-supervisor of Miguel Xavier Jesus Josefat Fernandes
1997 - 2000	Estudo do mecanismo de acção do antibiótico poliénico nistatina por técnicas de fluorescência Supervisor of Ana Isabel Abrantes Coutinho	Universidade de Lisboa Faculdade de Ciências, Portugal
1996 - 1999	Transferência de energia em sistemas modelo de membranas Supervisor of Luís Miguel Santos Loura	Universidade de Lisboa Instituto Superior Técnico, Portugal
1996 - 1999	Estudos estruturais em sistemas modelo de membranas biológicas. Interacção com poliósidos e fármacos Co-supervisor of Nuno Fernando Duarte Cordeiro Correia dos Santos	Universidade de Lisboa Faculdade de Ciências, Portugal
1990 - 1993	Fotoionização e fotoprotonação de sistemas aromáticos em solução: Um estudo de transientes por fotólise de laser de um e dois pulsos Co-supervisor of Joaquim Luís Bernardes Martins de Faria	Universidade de Lisboa Instituto Superior Técnico, Portugal
1990 - 1993	Modelo de acção bioquímica do antibiótico filipina Supervisor of Miguel Augusto Rico Botas Castanho	Universidade de Lisboa Instituto Superior Técnico, Portugal
1986 - 1989	Estudo dos efeitos orientacional e geométrico em transferência de energia electrónica com aplicação ao meio micelar Supervisor of Mário Nuno de Matos Sequeira Berberan e Santos	Universidade de Lisboa Instituto Superior Técnico, Portugal

Event organisation

	Event name Type of event (Role)	Institution / Organization
2019 - Current	Co-organizer of the Symposium ““Biophysics of Membrane Oxidation" on the “Joint 12th EBSA / 10th ICBP-IUPAP Biophysics Congress” (2019 - 2019) Congress (Co-organisor)	European Biophsyics Societies' Association (EBSA), United Kingdom

Event participation

	Activity description Type of event	Event name Institution / Organization
2019 - Current	Science Outreach activities "Museu Nacional de Histópria Natural" Exhibition	Feira de Ciência/Festa no Museu- Dia Mundial da Criança Universidade de Lisboa Museu Nacional de História Natural, Portugal
2019 - Current	Seminars on fluorescence and microscopy (Plataforma Portuguesa de Bioimagem) Other	Microscopy Open Day (MICRODia) Plataforma Portuguesa de Bioimagem, Portugal
2008 - Current	Science Outreach activities "Olympics of Chemistry" Conference	Portuguese Chemical Society
2020 - 2020	Conference: "Moléculas, materiais e seres luminosos" Conference	Laboratórios Abertos do DEQ Universidade de Lisboa Instituto Superior Técnico, Portugal
2019 - 2019	Participation in "Noite Europeia dos Investigadores (NEI) 2019" Festival	Noite Europeia dos Investigadores (NEI) 2019

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2019	Lipid-lipid interactions-in bilayers containing polar sphinglipids, ceramide and cholesterol President of the jury	Emilio José González Ramírez (PhD) Universidad del País Vasco, Spain
2019	Model Phospholipid membranes: Physical properties, interactions and oxidation (Thesis) Arguer	André Schroeder (Aggregation) Centre National de la Recherche Scientifique, France
2018	ABC and MFS transporters involved in multistress defense mechanisms in the model eukaryote saccharomyces cerevisiae: Roles of Pdr18 and Tpo1 (Thesis) Arguer	Cláudia Sofia Pires Godinho (PhD) Universidade de Lisboa Instituto Superior Técnico, Portugal
2018	Réorganisation des lipides des membranes par des peptides vecteurs d'internalisation cellulaire (Thesis) Main arguer	Cláudia Almeida (PhD) Polytech Sorbonne, France
2018	Physical Chemistry of Colloidal Systems	Eduardo Jorge Figueira Marques (Aggregation) Universidade do Porto, Portugal
2018	Structure-function relationship of pulmonar surfactant protein SP-B: Participation in protein complexes and in alveolar homeostasis (Thesis) Main arguer	Marta Martínez Calle (PhD) Universidad Complutense de Madrid, Spain

Association member

	Society Organization name	Role
2017 - Current	International Union of Pure and Applied Biophysics (IUPAB)	President-Elect
2014 - Current	Council Member of “LAFeBS” (Latin American Federation of Biophysical Societies)	Council Member
2000 - Current	Centro de Biología Molecular y Celular (Elche University-Spain)	Associate Researcher
1999 - Current	UNESCO Chair of Biophysics (Argentina)	Faculty Member
2013 - 2019	Scientific Coordinator of Grupo III of CQFM/IST – “Fluorescence and Biosystems"	Scientific Coordinator
2010 - 2016	European Biophysical Societies' Association (EBSA)	President of EBSA and Member of the Executive Committee

Committee member

	Activity description Role	Institution / Organization
2014 - Current	Member of the Scientific Committee "Biofisika", Basque Center for Biophysics Member	Biofisika Basque Center for Biophysics, Spain
2013 - Current	Member of the “Supervisory Board”, “Recruitment Committee” e “Mentorship Advisory Board (Research)” of the Doctoral Program “Medical Biochemistry and Biophysics” (M2B-PhD) (FCT) Coordinator	Fundação para a Ciência e a Tecnologia, Portugal
1995 - Current	Founder and President of the General Assembly of the "Portuguese Biophysical Society"	Portuguese Biophysical Society, Portugal
2013 - 2017	Member of the Scientific Council for the Exact Sciences and Engineering (FCT, Portugal) Member	Fundação para a Ciência e a Tecnologia, Portugal
2013 - 2015	Member of the Scientific Committee (PHYCHEMA)- Physics, Chemistry, Materials Member	Science Europe, Belgium
2009 - 2013	Member of FEBS (Federation of European Biochemical Societies) Publication Committee Member	Federation of European Biochemical Societies, United Kingdom
2006 - 2009	Member of the “Executive Board”and “Cluster co-coordinator” of the “European Marie-Curie Action EST “BioMem”	European Union, Belgium
1976 - 1977	Member of the "Pedagogical Council" of IST Member	Universidade de Lisboa Instituto Superior Técnico, Portugal

Conference scientific committee

	Conference name	Conference host
2018 - Current	6th International Iberian Biophysics Congress and X Iberoamerican Congress of Biophysics	Portuguese and Spanish Biophysical Societies
2018 - 2018	Lisbon Biophysics Networking Meeting: From Protein Dynamics to Membrane Biophysics	Univerisdade de Lisboa Instituto Superior Técnico
2018 - 2018	Luso-Brazilian Workshop on SAXS and fluorescence methodologies on biological systems	Universidade de Lisboa Instituto Superior Técnico

Consulting

	Activity description	Institution / Organization
2013 - 2013	Expert advice for "Initiative for Science in Europe"	Initiative for Science in Europe, Belgium

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
1981 - 2017	Structural Biology	(Mestrado integrado)	Universidade de Lisboa Instituto Superior Técnico, Portugal
1981 - 2017	Molecular Biophysics	(Mestrado integrado)	Universidade de Lisboa Instituto Superior Técnico, Portugal
1970 - 2017	General Chemistry	(Mestrado integrado)	Universidade de Lisboa Instituto Superior Técnico, Portugal

Evaluation committee

	Activity description Role	Institution / Organization	Funding entity
2020 - Current	H2020-FETOPEN-2014-2015-RIA-686637 Evaluator	European Commission, Belgium	European Commission
2017 - Current	Evaluator for "Academy of Finland" Evaluator	Suomen Akatemia, Finland
2013 - Current	Evaluator for "Fundação para a Ciência e Tecnologia" (FCT) Evaluator	Fundação para a Ciência e a Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia
2010 - Current	Evaluator for “CASP/FAPERJ” (Brasil). Evaluator	Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro, Brazil	Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2007 - Current	Evaluator for FONCyT (Fondo para la Investigación Científica y Tecnológica) Evaluator	Fondo para la Investigación Científica y Tecnológica, Argentina	Fondo para la Investigación Científica y Tecnológica
2006 - Current	Evaluator for European Marie Curie Actions Fellowships (EIF, OIF, IIF) (Panel Life Sciences), RTN, EST Evaluator	European Union, Belgium	European Union
2019 - 2019	Jury Member for Tenure-Eligible Lecturer in Physical Chemistry-Pharmacy Evaluator	Universitat Autónoma de Barcelona Departament de Farmacologia Terapèutica i Toxicologia, Spain	Universitat Autònoma de Barcelona
2019 - 2019	Jury Member for Professor (Química-Física/Faculdade de Farmácia) Universidade de Barcelona Evaluator	Universitat de Barcelona Departament de Química-Física, Spain	Universitat de Barcelona Departament de Farmacologia i Química Terapèutica
2019 - 2019	Evaluator for "Swedish Research Council” (Natural and Engineering Sciences) Evaluator	Vetenskapsradet, Sweden	Vetenskapsradet
2018 - 2018	Project Evaluator (Academy of Czech Republic) Evaluator	Grantová agentura Ceské republiky, Czech Republic	Akademie ved Ceske republiky
2018 - 2018	Project evaluator for Slovenian Research Agency (ARRS) Evaluator	Slovenian Research Agency (ARRS) , Slovenia	Slovenian Research Agency (ARRS)
2018 - 2018	Avaliador for Shanti Swarup Bhatnagar Prize for Science and Technology Evaluator	Council of Scientific and Industrial Research (CSIR), India	Council of Scientific and Industrial Research (CSIR).
2018 - 2018	Evaluator of “Council of Scientific & Industrial Research" (CSIR) Evaluator	Council of Scientific & Industrial Research (CSIR) , India
2018 - 2018	Evaluator for "Agence Nationale de la Recherche" (ANR) (France) Evaluator	Agence nationale de la recherche, France
2018 - 2018	Evaluator for the "Shanti Swarup Bhatnagar Prize for Science and Technology" (India) Evaluator	Council of Scientific and Industrial Research (CSIR), India	Council of Scientific and Industrial Research (CSIR)
2018 - 2018	Jury Member for "Professor in Physics of Biological Systems" (Univ. Sao Paulo, Brazil) Member	Universidade de São Paulo, Brazil	Universidade de São Paulo
2017 - 2017	Evaluator for “National Science Foundation” Evaluator	National Science Foundation, United States	National Science Foundation
2016 - 2016	Project evaluator for Volkswagen Foundation Evaluator	VolkswagenStiftung, Germany	VolkswagenStiftung
2016 - 2016	Evaluator for “Projetti Fondazione Cassa di Risparmio di Modena”; Evaluator	Projetti Fondazione Cassa di Risparmio di Modena , Italy
2016 - 2016	Member of "Comissão de Visita do Processo de Apreciação das Actividades de Investigação e Ensino do Departamenhto de Bioengenharia do IST" Evaluator	Universidade de Lisboa Instituto Superior Técnico, Portugal
2015 - 2015	Evaluator for "The Wellcome Trust/DBT India Alliance" Evaluator	The Wellcome Trust/DBT India Alliance, India
2015 - 2015	Evaluator for "Shota Rustaveli National Science Foundation" (Georgia)	Shota Rustaveli National Science Foundation, Georgia
2015 - 2015	Evaluator for "National Council for Scientific and Technological Development" (CNPq) Brazil Evaluator	Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil	Conselho Nacional de Desenvolvimento Científico e Tecnológico
2013 - 2013	Evaluator for "Investigadores FCT" Evaluator	Fundação para a Ciência e a Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia
2012 - 2012	Evaluator for Engineering and Physical Sciences Research Council (EPSRC)	Engineering and Physical Sciences Research Council (EPSRC), United Kingdom	Engineering and Physical Sciences Research Council (EPSRC)
2011 - 2011	Evaluator of TWAS “The Third World Academy of Sciences”; Evaluator	World Academy of Sciences, Italy	World Academy of Sciences
2010 - 2010	Evaluator of "Acções-Integradas" of "Fundação das Universidades Portuguesas" Evaluator	Fundação das Universidades Portuguesas, Portugal	Fundação das Universidades Portuguesas
2010 - 2010	Evaluator of Outreach Activities of "Universidade Federal do Rio de Janeiro" at Amazonia (Oriximiná) Evaluator	Universidade Federal do Rio de Janeiro, Brazil	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
2008 - 2010	Evaluator for "Avaliação Final dos Projectos de Química e Bioquímica" (FCT) Evaluator	Fundação para a Ciência e a Tecnologia, Portugal	Fundação para a Ciência e a Tecnologia
2009 - 2009	Evaluator of "Netherlands Organization for Scientific Research” (NWO) – Vici Program Evaluator	"Netherlands Organization for Scientific Research" (NWO), Netherlands	"Netherlands Organization for Scientific Research" (NWO)
2009 - 2009	Evalauator for "Universidade Federal do Rio de Janeiro" (UFRJ) Evaluator	Universidade Federal do Rio de Janeiro, Brazil	Universidade Federal do Rio de Janeiro
2009 - 2009	Evaluator of "Departamento de Bioquímica" of "Universidade Federal do Rio de Janeiro" (Brazil) Evaluator	Universidade Federal do Rio de Janeiro, Brazil	Universidade Federal do Rio de Janeiro
2008 - 2008	Evaluator for "Colciencias" (Colombia) Evaluator	Sistema Nacional de Ciencia Tecnología e Innovación, Colombia	Sistema Nacional de Ciencia Tecnología e Innovación
2007 - 2007	Evaluator for INTAS (International Association for the Promotion of Co-operation with Scientists from the New Independent States of the former Soviet Union); Evaluator	(International Association for the Promotion of Co-operation with Scientists from the New Independent States of the former Soviet Union); , Belgium	European Union
2006 - 2006	Evaluator of COST (European Co-operation in the field of Scientific and Technical Research) Action Evaluator	European Union, Belgium
2006 - 2006	Evaluator for "Georgian National Science Foundation" Evaluator	Georgian National Science Foundation, Georgia	Georgian National Science Foundation

Journal scientific committee

	Journal title (ISSN)	Publisher
2020/07/01 - Current	Membranes (ISSN 2077-0735)	MDPI
2018 - Current	International Journal of Molecular Sciences (1422-0067)	MDPI AG
2009 - Current	Biophysical Reviews (1867-2469)	Springer-Verlag
2009 - Current	Frontiers in Membrane Physiology and Biophysics	Frontiers
2008 - Current	Biochimica et Biophysica Acta (BBA) - Biomembranes (0005-2736)	Elsevier
2008 - Current	Chemistry and Physics of Lipids (0009-3084)	Elsevier

Other jury / evaluation

	Activity description	Institution / Organization
2011 - 2017	Member of the Jury of “Gregorio Weber International Prize in Biological Fluorescence”
2013 - 2013	Member of the Jury of the award “Prémio Fundação António de Almeida”, Universidade do Porto	Universidade do Porto, Portugal

Distinctions

Award

2013	Distinguished Visitor Universidad Nacional de Córdoba, Argentina
2013	Excellent Professor Universidade de Lisboa Instituto Superior Técnico, Portugal
2012	Excellent Professor Universidade de Lisboa Instituto Superior Técnico, Portugal
2011	Honorary Member Sociedad Biofísica de España, Spain
2008	Prémio Científico UTL/Santander Totta Universidade de Lisboa Instituto Superior Técnico, Portugal
2007	“Prémio Científico UTL / Santander Totta”- Biophysics and Physics - Honorary mention Universidade de Lisboa Instituto Superior Técnico, Portugal
2006	Prémio União Latina Universidade de Lisboa, Portugal
2005	Estímulo à Excelência Fundação para a Ciência e a Tecnologia, Portugal

Title

2020	Emeritus Member Biophysical Society, United States