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________ ACADEMIC BACKGROUND & WORK EXPERIENCE________ Marcela Segundo received her BSc in Microbiology (1997) and her PhD in Biotechnology, with specialization in Chemistry (2002), from the College of Biotechnology, Portuguese Catholic University. In 2022, she obtained her habilitation (agregação) in Pharmaceutical Sciences, specialization in Analytical Chemistry. She joined REQUIMTE, Portuguese Government Associate Laboratory for Green Chemistry, Clean technologies and processes (LAQV/REQUIMTE) as Researcher in 2003. In 2006, she joined the Department of Chemistry, Faculty of Pharmacy, U.Porto as lecturer, promoted to Professor Auxiliar in 2011 and to Professor Associado in 2024. She is currently the Chair of DAC-EuChemS - European Chemical Society (2023-2025) and member of the Executive Board of Faculty of Pharmacy, U.Porto since 2019. In 2013 she has established her independent research group in Separation Science and High-throughput Screening and she was the head of the Analytical Development Group at LAQV/REQUIMTE (2018-2024). Her current research focuses on automation of sample treatment prior to chromatographic analysis, and on development of high-throughput microchemical methods for food, environmental and biological samples. ________ EXPERIENCE IN SUPERVISION________ Overall, she has supervised more than 80 researchers at different stages of their scientific career/training. She has successfully supervised 12 PhD thesis and is currently supervisor of 5 PhD students. She also participates in PhD programs funded by FCT, namely as member of the Direction Board of SANFEED program - Sustainable Animal Nutrition and Feeding, as member of the Supervision Board of BiotechHealth program - International Doctoral Programme in Molecular and Cellular Biotechnology Applied to Health Sciences. She has also supervised 13 MSc thesis, and 13 research project students in the scope of curricular unit Project (6 ECTS - MSc in Pharmaceutical Sciences, U.Porto). She was responsible for the scientific supervision of 5 post-doc researchers (FCT grant) and other grant holders in the scope of funded research projects, including 3 post-docs and 14 MSc researchers. She has received 13 graduate and 6 undergraduate students from other countries for research (3-9 months) in her lab and she has current cooperation with groups in Germany, Spain, Australia, Brazil, Argentina, and Czech Republic. ________ SCIENTIFIC PRODUCTION________ Her scientific production comprises 14 book chapters, > 160 peer-reviewed papers in international journals, with over 4200 citations (h-index 36). She is the first author in 9 papers and corresponding/senior author of 75 papers. Among all papers, > 70% were published in top (Q1) peer-reviewed journals of Analytical Chemistry and Biochemical Research Methods areas including Nature Protocols, TRAC-Trends in Anal. Chem., Analytical Chemistry, and Analytica Chimica Acta. Her work was well received by the scientific community, testified by several awards received for papers presented in scientific conferences and the FIA Award for Science attributed by the Japanese Association for Flow Injection Analysis in 2016. ________ PARTICIPATION IN PROJECTS________ She was principal investigator of 6 projects, including PTDC/CTA-AMB/31756/2017, (2018-2021, Screening of antibiotic contamination by mobile devices - 230 kEuro) and PTDC/AAC-AMB/104882/2008 (2010-2013, Development and application of fast methodology for evaluation of UV filters levels in coastal bathing waters - 185 kEuro), both funded by Portuguese Foundation of Science and Technology. She is/was member of research team in other 29 projects, including two projects for high-tech equipment and three other projects led by industrial partners. In 2015 she has received the prize Acredita Portugal and the Prémio Empreendedorismo e Inovação Crédito Agrícola, acknowledging her participation in technology transfer to industrial partners.
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Identification

Personal identification

Full name
Marcela Alves Segundo

Citation names

  • Segundo, Marcela

Author identifiers

Ciência ID
0A14-B7A6-E805
ORCID iD
0000-0003-2938-0214
Researcher Id
A-1009-2010
Scopus Author Id
6602384941

Email addresses

  • msegundo@ff.up.pt (Professional)

Telephones

Telephone
  • 220428676 (Professional)

Addresses

  • LAQV/REQUIMTE, Laboratório de Química Aplicada, Faculdade de Farmácia, U.Porto, R. Jorge Viterbo Ferreira, 228, 4050-313, Porto, Porto, Portugal (Professional)

Knowledge fields

  • Exact Sciences - Chemical Sciences
  • Natural sciences - Other Natural Sciences

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2)
Spanish; Castilian Advanced (C1) Advanced (C1) Elementary (A2) Advanced (C1) Advanced (C1)
French Elementary (A2) Elementary (A2) Elementary (A2) Elementary (A2) Elementary (A2)
German Beginner (A1) Elementary (A2) Beginner (A1) Beginner (A1) Beginner (A1)
Italian Elementary (A2) Upper intermediate (B2) Elementary (A2) Upper intermediate (B2) Elementary (A2)
Education
Degree Classification
2022/09/15
Concluded
 Pharmaceutical Sciences (Título de Agregado)
Major in Química Analítica
Universidade do Porto Faculdade de Farmácia, Portugal
 Aprovado por unanimidade
2002/04/08
Concluded
 Biotechnology with specialization in Chemistry (Doutoramento)
Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
"Flow Systems based on Sequential Injection and Multisyringe for Wine Analysis" (THESIS/DISSERTATION)
 Approved by unanimity
1997/07
Concluded
 Microbiology (Licenciatura)
Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal
 18/20
Affiliation

Science

Category
Host institution 		Employer
2018/01 - Current Researcher (Research) REQUIMTE LAQV Porto, Portugal
Universidade do Porto Faculdade de Farmácia, Portugal
2014/01 - 2017/12 Researcher (Research) REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
2006/02 - 2013/12 Researcher (Research) REQUIMTE, Portugal
2003/01 - 2006/01 Auxiliary Researcher (Research) REQUIMTE, Portugal
2002/04 - 2002/12 Researcher (Research) Portuguesa Centro de Biotecnologia e Química Fina, Portugal

Teaching in Higher Education

Category
Host institution 		Employer
2024/01 - Current Associate Professor (University Teacher) Universidade do Porto Faculdade de Farmácia, Portugal
2011/07 - 2023/12 Assistant Professor (University Teacher) Universidade do Porto Faculdade de Farmácia, Portugal
2007/03 - 2011/07 Assistant (University Teacher) Universidade do Porto Faculdade de Farmácia, Portugal
2006/02 - 2007/03 Trainee Assistant (University Teacher) Universidade do Porto Faculdade de Farmácia, Portugal
1998/03 - 2002/12 Trainee Assistant (University Teacher) Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal

Positions / Appointments

Category
Host institution 		Employer
2023/01/01 - Current Chair to the Division of Analytical Chemistry EuChemS, Belgium
2019/03 - Current Member of Executive Board Universidade do Porto Faculdade de Farmácia, Portugal
2014/07 - Current Member of Direction Board - FCT PhD program SANFEED REQUIMTE, Portugal
2018/01 - 2024/02 Head of Analytical Chemistry Group REQUIMTE - Associated Laboratory for Green Chemistry, Portugal
2018/01 - 2022/12/31 Secretary to the Division of Analytical Chemistry EuChemS, Belgium
2018/09 - 2019/03 Member of Council of Representatives Universidade do Porto Faculdade de Farmácia, Portugal
2016/06 - 2018/03 President of Analytical Chemistry Division Sociedade Portuguesa de Química, Portugal
Projects

Grant

Designation Funders
2021/09/01 - 2024/08/31 Greenness of official standard sample preparation methods
2021-015-2-500
Principal investigator
International Union of Pure and Applied Chemistry
Ongoing
2022/06 - 2022/06 Grant Papers@USA
Papers@USA
Grant awardee
Fundação Luso-Americana para o Desenvolvimento
Concluded
2019/03 - 2019/03 Iacobus grant for mobility
PDI-218
Grant awardee - mobility
Universidade da Coruña Facultade de Ciencias, Spain
 Comissao de Coordenacao e Desenvolvimento Regional do Norte

Xunta de Galicia
Concluded
2012 - 2013 Method for fast screening of chloranisoles in cork
PP_IJUP2012_SOGRAPE
Principal investigator
Universidade do Porto

Sogrape S.A.
Concluded
2012/01 - 2012/12 Synthesis and application of novel molecularly imprinted materials for determination of an aromatase inhibitor in biological samples
IJUP_2012_04
Researcher
Universidade do Porto
Concluded
2011/01 - 2011/12 Evaluation of anti-Leishmania compounds released from polymeric nanoparticles
IJUP_2011_06
Researcher
Universidade do Porto
Concluded
2008 - 2009 Characterization and evaluation of performance of nano-structured sorbents for automatic extraction and preconcentration of pollutants
E-48/2009
Principal investigator
Conselho de Reitores das Universidades Portuguesas
Concluded
1997/10 - 2001/09 FCT PhD grant - PRAXIS XXI BD/13648/97
PRAXIS XXI BD/13648/97
PhD Student Fellow
Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2021/01/01 - 2025/12/31 Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos
LA/P/0008/2020
10.54499/LA/P/0008/2020
Universidade de Lisboa Instituto Dom Luiz, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Universidade NOVA de Lisboa, Portugal

Unidade de Investigação Vidro e Cerâmica para as Artes, Portugal

Universidade de Aveiro, Portugal

Universidade de Évora, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2023/01/01 - 2024/06/30 Analytics2H - Estratégias analíticas avançadas baseadas em impressão 3D para desvendar a transição saúde-doença
2022.06012.PTDC
Researcher
Fundação para a Ciência e a Tecnologia
2020/09/01 - 2023/08/31 AgriFood XXI - Development and consolidation of research in the agrifood sector in Northern Portugal
NORTE-01-0145-FEDER-000041
Researcher
European Regional Development Fund
2021/11/01 - 2023/04/30 ACT4Nano - Analytical strategies for accelerating the clinical translation of nanoparticles
EXPL/QUI-QAN/0360/2021
Researcher
Fundação para a Ciência e a Tecnologia
2018 - 2022 EFSA-CDN - Efficiency and safety improvement of current drugs and nutraceuticals: advanced methods ¿ new challenge
CZ.02.1.01/0.0/0.0/16_019/0000841
Invited Scientist Fellow
Czech Ministry of Education, Youth and Sports
Ongoing
2017 - 2022 STARSS - Establishment of Specialized Team for Advanced Research on Separation Science
CZ.02.1.01/0.0/0.0/15_003/0000465
Invited Scientist Fellow
European Regional Development Fund
Ongoing
2018/07/01 - 2021/12/31 Screening of antibiotic contamination by mobile devices
PTDC/CTA-AMB/31756/2017
Principal investigator
Instituto de Engenharia de Sistemas e Computadores Tecnologia e Ciência, Portugal

Universidade do Porto, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/10 - 2020/09 DEPCAT - Demonstration of new EquiPment involving integrated CATalytic processes for the treatment of organic pollutants and disinfection of water
NORTE-01-0247-FEDER-033330
Researcher
European Regional Development Fund

Adventech
Ongoing
2018 - 2020 AQUASOME - Exposome assessment of emerging contaminants in the aquatic environment
CTM2017-84763-C3-2-R
Invited Scientist Fellow
European Regional Development Fund
Ongoing
2019/01/01 - 2019/12/31 Unidade de Ciências Biomoleculares Aplicadas
UID/Multi/04378/2019
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal

Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016/12 - 2019/11 MinDog - Organic minerals in petfood
POCI-01-0247-FEDER-017616
Researcher
European Regional Development Fund

Soja de Portugal

Alltech International
2016/06/01 - 2019/06/01 Grapevine cultivars metabolic modelling combining weather, soil and non-invasive in-situ vibrational spectroscopy data
PTDC/AGR-PRO/6817/2014
Researcher
Universidade de Lisboa Instituto Superior de Agronomia, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2016/01 - 2017/12 Immuno-based molecular recognition combined to mesofluidics for solid-phase renewable assays
E-20/16
Principal investigator
Deutscher Akademischer Austauschdienst

Conselho de Reitores das Universidades Portuguesas
2015/01 - 2017/09 Plataformas fluidicas de impresion 3D para tests de biodisponibilidad oral de contaminantes ambientales usando una metodologia holistica
CTM2014-61553-EXP
Invited Scientist Fellow
Ministerio Español de Economía y Competitividad
Concluded
2015/01 - 2017/09 Nuevas metodologias para evaluar el impacto de contaminantes emergentes en ecosistemas marinos y consumidores
CTM2014-56628-C3-3-R
Invited Scientist Fellow
Ministerio Español de Economía y Competitividad
Concluded
2014/01 - 2015/06 [Creation of Laboratory of Chemical Forensics in North of Portugal] Dotar a Região Norte de um Laboratório de Química Forense
NORTE-07-0162-FEDER-000124
Researcher
European Regional Development Fund
Concluded
2014/01 - 2015/06 [Creation of Unit for advanced characterization of mechanisms of transport and membranes in North of Portugal] Dotar a região Norte de uma unidade de caracterização avançada de membranas- estrutura e mecanismos de transporte
Norte-07-0162-FEDER-000088
Researcher
European Regional Development Fund
Concluded
2014/01 - 2015/06 NEPCAT - New EquiPment involving integrated CATalytic processes for the treatment of organic pollutants and disinfection of water
NORTE-07-0202-FEDER-038900
Researcher
European Regional Development Fund
Concluded
2013/01 - 2015/06 Nanochemistry
NORTE-07-0124-FEDER-000067
Researcher
European Regional Development Fund
Concluded
2010/06/01 - 2013/11/30 Development and application of fast methodology for evaluation of UV filters levels in coastal bathing waters
PTDC/AAC-AMB/104882/2008
Principal investigator
Universidade do Porto Instituto de Ciências e Tecnologias Agrárias e Agro-Alimentares, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade Católica Portuguesa, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2011/01/01 - 2012/12/31 Projecto Estratégico - LA 6 - 2011-2012
PEst-C/EQB/LA0006/2011
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2010/01 - 2012/12 Automated analytical approaches based on dynamic fractionation for assessment of bioavailable fractions of environmental pollutants in soils, sediments and solid wastes
CTM2010-17214
Invited Scientist Fellow
Gobierno de España Ministerio de Ciencia e Innovación
Concluded
2005/01/01 - 2008/06/30 Assessing the dietary exposure of Portuguese consumers to acrylamide. Improvement, development and validation of rapid screening procedures and confirmatory analytical methodologies.
POCI/AGR/61543/2004
Researcher
REQUIMTE LAQV Porto, Portugal

Universidade do Porto Instituto de Ciências e Tecnologias Agrárias e Agro-Alimentares, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2006/01 - 2006/12 Fluorescence facility for the Centro de Química da Universidade do Porto
REEQ/1079/QUI/2006
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2005/01 - 2006/12 Bioassay-guided isolation and identification of anti-inflammatory and antioxidant compounds from a medicinal extract of Pedilanthus tithymaloides
REQUIMTE-06
Principal investigator
Rede de Química e Tecnologia Laboratório Associado para a Química Verde
Concluded
2001 - 2004 [Development and application of expeditious methodologies for control and safety in agro food sector] Desenvolvimento e Aplicação de Metodologias Expeditas de Controlo e Segurança no Sector Agro-alimentar
AGRO 273
Researcher
Instituto Nacional de Investigação Agrária e das Pescas
Concluded
1999 - 2001 INSIA [Development of instrumentation based on SIA for water and wine analysis] Desenvolvimento de Instrumentação Baseada em SIA para Análise de Água e de Vinho
PO76-P31B-09/97
Researcher
Agência Nacional de Inovação SA

Paralab
Concluded
Outputs

Publications

Book chapter
  1. S. Peixoto, Patrícia; Machado, Ana; P. Oliveira, Hélder; A. Bordalo, Adriano; A. Segundo, Marcela. "Paper-Based Biosensors for Analysis of Water". In Biosensors for Environmental Monitoring. IntechOpen, 2019.
    10.5772/intechopen.84131
  2. Almeida, M.I.G.S.; Barreiros, L.; Kolev, S.D.; Segundo, M.A.; Almeida, M. Inês G.S.; Barreiros, Luisa; Kolev, Spas D.; Segundo, Marcela A.. "Salivary cotinine assays". In Neuroscience of Nicotine, 411-418. Elsevier, 2019.
    10.1016/b978-0-12-813035-3.00050-2
  3. Magalhães, Luís M.; Ramos, Inês I.; Barreiros, Luísa; Reis, Salette; Segundo, Marcela A.. "Kinetic matching approach for rapid assessment of endpoint antioxidant capacity". In Measurement of Antioxidant Activity & Capacity, 321-331. John Wiley & Sons, Ltd, 2017.
    10.1002/9781119135388.ch15
  4. Tóth, I.V.; Marques, S.S.; Magalhães, L.M.; Segundo, M.A.. "Determination of tartaric acid". 2015.
  5. Magalhñes, L.M.; Ramos, I.; Tóth, I.V.; Reis, S.; Segundo, M.A.. "Determination of antioxidant activity by fia and microplates". 2015.
  6. Segundo, M.A.; Barreiros, L.; Tóth, I.V.; Magalhães, L.M.. "Flow injection analysis: Recent developments and future trends". 2015.
  7. Segundo, M.A.; Tóth, I.V.; Magalhães, L.M.; Reis, S.. "Automatic flow injection analysis (FIA) determination of total reducing capacity in serum and urine samples". In Advanced Protocols in Oxidative Stress III. New York: Springer Science+Business Media, 2014.
    Published
  8. Segundo, M.A.; Castro, C.; Magalhães, L.M.; Reis, S.. "Determination of scavenging capacity against hydrogen peroxide: Recent trends on chemical methods". 2013.
  9. Segundo, Marcela A.; Osório, Marcelo V.; Oliveira, Hugo M.; Barreiros, Luísa; Magalhães, Luís M.. "CHAPTER 18. Assays of Riboflavin in Food using Solid-phase Extraction". In Food and Nutritional Components in Focus, 271-284. Royal Society of Chemistry, 2013.
    10.1039/9781849734714-00271
  10. A., Marcela; S. Almeida, M. Ines G.; M., Hugo. "Multisyringe Flow Injection Analysis for Environmental Monitoring: Applications and Recent Trends". In Environmental Monitoring. InTech, 2011.
    10.5772/28770
  11. Segundo, Marcela A.; Magalhães, Luís M.; Ribeiro, Joana P.N.; Lúcio, Marlene; Reis, Salette. "Determination of the Scavenging Scavenging Capacity Against Reactive Nitrogen Species by Automatic Flow Injection-Based Methodologies". In Methods in Molecular Biology, 91-104. Humana Press, 2010.
    10.1007/978-1-61737-964-2_8
  12. Reis, Salette; Lúcio, Marlene; Segundo, Marcela; Lima, José L. F. C.. "Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity". In Methods in Molecular Biology, 167-188. Humana Press, 2009.
    10.1007/978-1-60761-447-0_13
  13. Tóth, I.; Segundo, M.A.; Rangel, A.. "Chapter 18 Food, Beverages and Agricultural Applications". 2008.
    10.1016/S0166-526X(08)00618-1
Conference paper
  1. Carvalho, P.H.; Rocha, I.; Azevedo, F.; Peixoto, P.S.; Segundo, M.A.; Oliveira, H.P.. "Cost-Efficient Color Correction Approach on Uncontrolled Lighting Conditions". 2021.
    10.1007/978-3-030-89128-2_9
  2. Reis, P.; Carvalho, P.H.; Peixoto, P.S.; Segundo, M.A.; Oliveira, H.P.. "Mobile application for determining the concentration of sulfonamides in water using digital image colorimetry". 2021.
    10.1007/978-3-030-78095-1_34
  3. Carvalho, P.H.; Bessa, S.; Silva, A.R.M.; Peixoto, P.S.; Segundo, M.A.; Oliveira, H.P.. "Estimation of Sulfonamides Concentration in Water Based on Digital Colourimetry". 2019.
    10.1007/978-3-030-31332-6_31
Journal article
  1. Carolina Liana Ronca; Sara Marques; Prof. Dr. H.c. PhD Alberto Ritieni; Rafael Giménez-Martínez; Luisa Barreiros; Marcela Segundo. "Olive Oil Waste as a Source of Functional Food Ingredients: Assessing Polyphenolic Content and Antioxidant Activity in Olive Leaves". Foods (2024): https://www.mdpi.com/2304-8158/13/2/189.
    10.3390/foods13020189
  2. Joana R. B. Carvalho; Andreia N. Meireles; Sara S. Marques; Bruno J. R. Gregório; Inês I. Ramos; Eduarda M. P. Silva; Luisa Barreiros; Marcela Segundo. "Exploiting Kinetic Features of ORAC Assay for Evaluation of Radical Scavenging Capacity". Antioxidants (2023): https://www.mdpi.com/2076-3921/12/2/505.
    10.3390/antiox12020505
  3. Silva, L.A.P.; Campagnolo, S.; Fernandes, S.R.; Marques, S.S.; Barreiros, L.; Sampaio-Maia, B.; Segundo, M.A.. "Rapid and sustainable HPLC method for the determination of uremic toxins in human plasma samples". Analytical and Bioanalytical Chemistry 415 4 (2023): 683-694. http://www.scopus.com/inward/record.url?eid=2-s2.0-85143276652&partnerID=MN8TOARS.
    10.1007/s00216-022-04458-w
  4. Fernandes, S.R.; Meireles, A.N.; Marques, S.S.; Silva, L.; Barreiros, L.; Sampaio-Maia, B.; Miró, M.; Segundo, M.A.. "Sample preparation and chromatographic methods for the determination of protein-bound uremic retention solutes in human biological samples: An overview". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 1215 (2023): http://www.scopus.com/inward/record.url?eid=2-s2.0-85145963905&partnerID=MN8TOARS.
    10.1016/j.jchromb.2022.123578
  5. Marques, S.S.; Ramos, I.I.; Silva, C.; Barreiros, L.; Domingues, M.R.; Segundo, M.A.. "Lab-on-Valve Automated and Miniaturized Assessment of Nanoparticle Concentration Based on Light-Scattering". Analytical Chemistry 95 10 (2023): 4619-4626. http://www.scopus.com/inward/record.url?eid=2-s2.0-85148770194&partnerID=MN8TOARS.
    10.1021/acs.analchem.2c04631
  6. Machado, S.; Barreiros, L.; Graça, A.R.; Páscoa, R.N.M.J.; Segundo, M.A.; Lopes, J.A.. "A data mining tool for untargeted biomarkers analysis: Grapes ripening application". Chemometrics and Intelligent Laboratory Systems 233 (2023): http://www.scopus.com/inward/record.url?eid=2-s2.0-85145730136&partnerID=MN8TOARS.
    10.1016/j.chemolab.2022.104745
  7. Varandas, P.A.M.M.; Belinha, R.; Marques, S.S.; Cobb, A.J.A.; Serra, V.V.; Segundo, M.A.; Silva, E.M.P.. "Fluorescent head-labelled phospholipid coumarin bioconjugate as a chemical reporter for antioxidant protection in liposomes". Dyes and Pigments 218 (2023): http://www.scopus.com/inward/record.url?eid=2-s2.0-85161295529&partnerID=MN8TOARS.
    10.1016/j.dyepig.2023.111440
  8. Fernandes, S.R.; Barreiros, L.; Sampaio-Maia, B.; Miró, M.; Segundo, M.A.. "Total analysis system for the determination of uremic toxins in human plasma based on bead injection solid phase extraction hyphenated to mass spectrometry". Analytica Chimica Acta 1277 (2023): http://www.scopus.com/inward/record.url?eid=2-s2.0-85168508933&partnerID=MN8TOARS.
    10.1016/j.aca.2023.341668
  9. Machado, S.; Barreiros, L.; Graça, A.R.; Madeira, M.; Páscoa, R.N.M.J.; Segundo, M.A.; Lopes, J.A.. "Assessing the differences of two vineyards soils’ by NIR spectroscopy and chemometrics". Heliyon 9 12 (2023): http://www.scopus.com/inward/record.url?eid=2-s2.0-85178138990&partnerID=MN8TOARS.
    10.1016/j.heliyon.2023.e23000
  10. Cant, D.J.H.; Pei, Y.; Shchukarev, A.; Ramstedt, M.; Marques, S.S.; Segundo, M.A.; Parot, J.; et al. "Cryo-XPS for Surface Characterization of Nanomedicines". Journal of Physical Chemistry A 127 39 (2023): 8220-8227. http://www.scopus.com/inward/record.url?eid=2-s2.0-85173556161&partnerID=MN8TOARS.
    10.1021/acs.jpca.3c03879
  11. Román-Hidalgo, C.; Barreiros, L.; Villar-Navarro, M.; López-Pérez, G.; Martín-Valero, M.J.; Segundo, M.A.. "Electromembrane extraction based on biodegradable materials: Biopolymers as sustainable alternatives to plastics". TrAC - Trends in Analytical Chemistry 162 (2023): http://www.scopus.com/inward/record.url?eid=2-s2.0-85151572085&partnerID=MN8TOARS.
    10.1016/j.trac.2023.117048
  12. Marques, S.S.; Cant, D.J.H.; Minelli, C.; Segundo, M.A.. "Combining orthogonal measurements to unveil diclofenac encapsulation into polymeric and lipid nanocarriers". Analytica Chimica Acta 1262 (2023): http://www.scopus.com/inward/record.url?eid=2-s2.0-85153292064&partnerID=MN8TOARS.
    10.1016/j.aca.2023.341234
  13. Ana Merino Ribas; Ricardo Araujo; Luciano Pereira; Joana Campos; Luisa Barreiros; Marcela Segundo; Nádia Silva; et al. "Vascular Calcification and the Gut and Blood Microbiome in Chronic Kidney Disease Patients on Peritoneal Dialysis: A Pilot Study". Biomolecules (2022): https://www.mdpi.com/2218-273X/12/7/867.
    10.3390/biom12070867
  14. Patrícia S. Peixoto; Pedro H. Carvalho; Machado, A.; Luisa Barreiros; Adriano A. Bordalo; Helder P. Oliveira; Marcela Segundo. "Development of a Screening Method for Sulfamethoxazole in Environmental Water by Digital Colorimetry Using a Mobile Device". Chemosensors (2022): https://www.mdpi.com/2227-9040/10/1/25.
    10.3390/chemosensors10010025
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  129. Magalhães, L.M.; Santos, M.; Segundo, M.A.; Reis, S.; Lima, J.L.F.C.. "Flow injection based methods for fast screening of antioxidant capacity". Talanta 77 5 (2009): 1559-1566. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149520171&partnerID=MN8TOARS.
    10.1016/j.talanta.2008.10.034
  130. Magalhães, L.M.; Segundo, M.A.; Reis, S.; Lima, J.L.F.C.. "Methodological aspects about in vitro evaluation of antioxidant properties". Analytica Chimica Acta 613 1 (2008): 1-19. http://www.scopus.com/inward/record.url?eid=2-s2.0-40949083840&partnerID=MN8TOARS.
    10.1016/j.aca.2008.02.047
  131. Abreu, P.M.; Matthew, S.; González, T.; Vanickova, L.; Costa, D.; Gomes, A.; Segundo, M.A.; Fernandes, E.. "Isolation and identification of antioxidants from Pedilanthus tithymaloides". Journal of Natural Medicines 62 1 (2008): 67-70. http://www.scopus.com/inward/record.url?eid=2-s2.0-38749128028&partnerID=MN8TOARS.
    10.1007/s11418-007-0186-z
  132. Almeida, M.I.G.S.; Segundo, M.A.; Lima, J.L.F.C.; Rangel, A.O.S.S.. "Direct introduction of slurry samples in multi-syringe flow injection analysis: Determination of potassium in plant samples". Analytical Sciences 24 5 (2008): 601-606. http://www.scopus.com/inward/record.url?eid=2-s2.0-48849107858&partnerID=MN8TOARS.
    10.2116/analsci.24.601
  133. Magalhães, L.M.; Segundo, M.A.; Reis, S.; Lima, J.L.F.C.; Tóth, I.V.; Rangel, A.O.S.S.. "Automatic flow system for sequential determination of ABTS{radical dot}+ scavenging capacity and Folin-Ciocalteu index: A comparative study in food products". Analytica Chimica Acta 592 2 (2007): 193-201. http://www.scopus.com/inward/record.url?eid=2-s2.0-34248550131&partnerID=MN8TOARS.
    10.1016/j.aca.2007.04.028
  134. Magalhães, L.M.; Segundo, M.A.; Reis, S.; Lima, J.L.F.C.; Estela, J.M.; Cerdà, V.. "Automatic in vitro determination of hypochlorous acid scavenging capacity exploiting multisyringe flow injection analysis and chemiluminescence". Analytical Chemistry 79 10 (2007): 3933-3939. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249094815&partnerID=MN8TOARS.
    10.1021/ac0700068
  135. Manera, M.; Miró, M.; Estela, J.M.; Cerdà, V.; Segundo, M.A.; Lima, J.L.F.C.. "Flow-through solid-phase reflectometric method for simultaneous multiresidue determination of nitrophenol derivatives". Analytica Chimica Acta 600 1-2 SPEC. (2007): 155-163. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548846410&partnerID=MN8TOARS.
    10.1016/j.aca.2007.01.040
  136. Dasgupta, P.; Lima, J.L.F.C.; Saraiva, L.; Segundo, M.. "Foreword". Analytica Chimica Acta 600 1-2 SPEC. (2007): http://www.scopus.com/inward/record.url?eid=2-s2.0-34548849142&partnerID=MN8TOARS.
    10.1016/j.aca.2007.06.051
  137. Magalhães, L.M.; Segundo, M.A.; Siquet, C.; Reis, S.; Lima, J.L.F.C.. "Multi-syringe flow injection system for the determination of the scavenging capacity of the diphenylpicrylhydrazyl radical in methanol and ethanolic media". Microchimica Acta 157 1-2 (2007): 113-118. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846000261&partnerID=MN8TOARS.
    10.1007/s00604-006-0633-7
  138. Magalhães, L.M.; Segundo, M.A.; Reis, S.; Lima, J.L.F.C.; Rangel, A.O.S.S.. "Automatic method for the determination of Folin-Ciocalteu reducing capacity in food products". Journal of Agricultural and Food Chemistry 54 15 (2006): 5241-5246. http://www.scopus.com/inward/record.url?eid=2-s2.0-33746868852&partnerID=MN8TOARS.
    10.1021/jf060324s
  139. Oliveira, H.M.; Segundo, M.A.; Lima, J.L.F.C.; Grassi, V.; Zagatto, E.A.G.. "Kinetic enzymatic determination of glycerol in wine and beer using a sequential injection system with spectrophotometric detection". Journal of Agricultural and Food Chemistry 54 12 (2006): 4136-4140. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745742477&partnerID=MN8TOARS.
    10.1021/jf060229j
  140. Abreu, P.; Matthew, S.; González, T.; Costa, D.; Segundo, M.A.; Fernandes, E.. "Anti-inflammatory and antioxidant activity of a medicinal tincture from Pedilanthus tithymaloides". Life Sciences 78 14 (2006): 1578-1585. http://www.scopus.com/inward/record.url?eid=2-s2.0-32944458843&partnerID=MN8TOARS.
    10.1016/j.lfs.2005.07.037
  141. Almeida, M.I.G.S.; Segundo, M.A.; Lima, J.L.F.C.; Rangel, A.O.S.S.. "Potentiometric multi-syringe flow injection system for determination of exchangeable potassium in soils with in-line extraction". Microchemical Journal 83 2 (2006): 75-80. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646767430&partnerID=MN8TOARS.
    10.1016/j.microc.2006.02.004
  142. Magalhães, L.M.; Segundo, M.A.; Reis, S.; Lima, J.L.F.C.. "Automatic method for determination of total antioxidant capacity using 2,2-diphenyl-1-picrylhydrazyl assay". Analytica Chimica Acta 558 1-2 (2006): 310-318. http://www.scopus.com/inward/record.url?eid=2-s2.0-31044441881&partnerID=MN8TOARS.
    10.1016/j.aca.2005.11.013
  143. Segundo, M.A.; Magalhães, L.M.. "Multisyringe flow injection analysis: State-of-the-art and perspectives". Analytical Sciences 22 1 (2006): 3-8. http://www.scopus.com/inward/record.url?eid=2-s2.0-30744465962&partnerID=MN8TOARS.
    10.2116/analsci.22.3
  144. Almeida, M.I.G.S.; Segundo, M.A.; Lima, J.L.F.C.; Rangel, A.O.S.S.. "Multi-syringe flow injection system for the determination of available phosphorus in soil samples". International Journal of Environmental Analytical Chemistry 85 1 (2005): 51-62. http://www.scopus.com/inward/record.url?eid=2-s2.0-27744462658&partnerID=MN8TOARS.
    10.1080/03067310512331324718
  145. Oliveira, H.M.; Segundo, M.A.; Reis, S.; Lima, J.L.F.C.. "Multi-syringe flow injection system with in-line pre-concentration for the determination of total phenolic compounds". Microchimica Acta 150 2 (2005): 187-196. http://www.scopus.com/inward/record.url?eid=2-s2.0-21244450383&partnerID=MN8TOARS.
    10.1007/s00604-005-0345-4
  146. Gomes, D.M.C.; Segundo, M.A.; Lima, J.L.F.C.; Rangel, A.O.S.S.. "Spectrophotometric determination of iron and boron in soil extracts using a multi-syringe flow injection system". Talanta 66 3 (2005): 703-711. http://www.scopus.com/inward/record.url?eid=2-s2.0-18844444473&partnerID=MN8TOARS.
    10.1016/j.talanta.2004.12.011
  147. Segundo, M.A.; Oliveira, H.M.; Lima, J.L.F.C.; Almeida, M.I.G.S.; Rangel, A.O.S.S.. "Sample introduction in multi-syringe flow injection systems: Comparison between time-based and volume-based strategies". Analytica Chimica Acta 537 1-2 (2005): 207-214. http://www.scopus.com/inward/record.url?eid=2-s2.0-17144368442&partnerID=MN8TOARS.
    10.1016/j.aca.2005.01.009
  148. Almeida, M.I.G.S.; Segundo, M.A.; Lima, J.L.F.C.; Rangel, A.O.S.S.. "Multi-syringe flow injection system with in-line microwave digestion for the determination of phosphorus". Talanta 64 5 SPEC. IS (2004): 1283-1289. http://www.scopus.com/inward/record.url?eid=2-s2.0-7044229327&partnerID=MN8TOARS.
    10.1016/j.talanta.2004.04.006
  149. Segundo, M.A.; Lima, J.L.F.C.; Rangel, A.O.S.S.. "Automatic flow systems based on sequential injection analysis for routine determinations in wines". Analytica Chimica Acta 513 1 (2004): 3-9. http://www.scopus.com/inward/record.url?eid=2-s2.0-2442486670&partnerID=MN8TOARS.
    10.1016/j.aca.2003.11.061
  150. Segundo, M.A.; Rangel, A.O.S.S.. "Kinetic determination of L(-)malic acid in wines using sequential injection analysis". Analytica Chimica Acta 499 1-2 (2003): 99-106. http://www.scopus.com/inward/record.url?eid=2-s2.0-0344413677&partnerID=MN8TOARS.
    10.1016/S0003-2670(03)00885-7
  151. Silva, H.R.; Segundo, M.A.; Rangel, A.O.S.S.. "Use of a mixing chamber for sample preparation and multiple collection in sequential injection analysis: Determination of sulfate in wines". Journal of the Brazilian Chemical Society 14 1 (2003): 59-64. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037740975&partnerID=MN8TOARS.
  152. Segundo, M.A.; Rangel, A.O.S.S.. "Sequential injection flow system with improved sample throughput: Determination of glycerol and ethanol in wines". Analytica Chimica Acta 458 1 (2002): 131-138. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037193054&partnerID=MN8TOARS.
    10.1016/S0003-2670(01)01525-2
  153. Segundo, M.A.; Rangel, A.O.S.S.. "A gas diffusion sequential injection system for the determination of sulphur dioxide in wines". Analytica Chimica Acta 427 2 (2001): 279-286. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035951716&partnerID=MN8TOARS.
    10.1016/S0003-2670(00)01197-1
  154. Segundo, M.A.; Rangel, A.O.S.S.; Cladera, A.; Cerda, V.. "Multisyringe flow system: Determination of sulfur dioxide in wines". Analyst 125 8 (2000): 1501-1505. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033839807&partnerID=MN8TOARS.
    10.1039/b002225j
  155. Araújo, A.N.; Lima, J.L.F.C.; Rangel, A.O.S.S.; Segundo, M.A.. "Sequential injection system for the spectrophotometric determination of reducing sugars in wines". Talanta 52 1 (2000): 59-66. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034737995&partnerID=MN8TOARS.
    10.1016/S0039-9140(99)00338-0
Journal issue
  1. Segundo, Marcela. "Special Issue Analytical Techniques in the Pharmaceutical Sciences". Pharmaceuticals (2021): https://www.mdpi.com/journal/pharmaceuticals/special_issues/analytical_techniques_pharm.
    Published • Invited editor
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2023/05 - Current Study of bioaccessibility/bioavailability and interaction of emerging and priority pollutants: continuous flow methodologies and implications in biological membranes
Supervisor of Joel Sánchez Piñero
 Post-doc, REQUIMTE-LAQV/FFUP (Other)
REQUIMTE LAQV Porto, Portugal
2022/09 - Current Development of an electrochemical analytical method for the quality control of biopharmaceuticals based on monoclonal antibodies
Supervisor
 Mestrado em Controlo de Qualidade
Universidade do Porto Faculdade de Farmácia, Portugal
2021/09/01 - Current Exploring the potential of fluorescent carbon and quantum dots for highly specific detection by molecular recognition of virus miRNAs
Co-supervisor of Ana Luísa Rodrigues da Silva
 Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2021/04/01 - Current Implementation of reusable microfluidic devices embedding enzymes of the cytochrome P450 superfamily for in vitro metabolic assessment
Co-supervisor of Carina Sofia Pereira Vieira
 Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2021/01/01 - Current Development of analytical strategies for evaluation of biopharmaceuticals
Supervisor of Diana Raquel de Lima Cunha
 Doutoramento em Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2019/01 - Current Dual Mass-Fluorescence Tags for Lipidomics
Co-supervisor of Pedro António Martins Mira Varandas
 Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2018/01 - Current Total analysis systems based on mesofluidics coupled to tandem mass spectrometry
Supervisor of Sara Regina Rodrigues Fernandes
 Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2019/01 - 2023/05 Mesofluidic analytical systems based on renewable solid-phase chemistry and molecular recognition
Supervisor of Bruno José Rodrigues Gregório
 Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2017/01 - 2023/05 Grapevine cultivars metabolic modeling combining metabolic, (micro)climate, soil and spectroscopy data
Co-supervisor of Sandia Santos Machado
 Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2019/09/01 - 2021/12/07 Implementação de ensaios in vitro para avaliação da atividade antioxidante de suplementos alimentares contendo L-carnitina
Co-supervisor of Hellen da Silva Cabral
 Mestrado em Controlo de Qualidade
Universidade do Porto Faculdade de Farmácia, Portugal
2019/09 - 2020/12/04 Evaluation of uremic solutes in biological samples of Chronic Kidney Disease patients
Supervisor of Luís António Miranda de Sousa Pinto Silva
 Contaminação e Toxicologia Ambientais (Master)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2016/01 - 2020/09/04 Nanometrics - analytical developments targeted to nanostructures
Supervisor of Sara Cristina da Silva Marques
 Biotecnologia Molecular e Celular Aplicada às Ciências da Saúde (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2016/01 - 2020/07/29 Improving bioavailability of trace elements in dog feed: The role of organic sources
Co-supervisor of Ana Margarida Batista Pereira
 Ciência Animal (PhD)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2018/09 - 2019/07 Chemical and biological dual sensor for evaluation of sulfonamides in environmental waters
Supervisor of Ana Rosa Moreira da Silva
 Bioengenharia (Master)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2015/09 - 2019/06 Molecular recognition strategies combined to mesofluidics for solid-phase renewable assays
Supervisor of Inês Isabel Barros Moreira Ramos
 Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2013/12 - 2018/10 Automation of biochemical assays using lab-on-valve and bead injection techniques
Co-supervisor of Luisa Maria Ribeiro da Silva Barreiros
 Post-Doc, REQUIMTE/FFUP, grant FCT SFRH/BPD/89668/2012 (Other)
Universidade do Porto Faculdade de Farmácia, Portugal
2017/09 - 2018 Development and application of a high-throughput method for determination of zinc in pet food
Supervisor of Rute Isabel Correia Martins
 Controlo de Qualidade (Master)
Universidade do Porto Faculdade de Farmácia, Portugal
2016/09 - 2017/07 Digital image colorimetry for determination of sulfonamides in water
Co-supervisor of Paulo Jorge Teixeira Silva
 Engenharia Electrotécnica e de Computadores (Master)
Universidade do Porto Faculdade de Engenharia, Portugal
2013/09 - 2017/06 Micro- and Nano-flow approaches for monitoring the fate and environmental impacts of veterinary antibiotics
Co-supervisor of Patrícia Lisandri de Souza Peixoto
 Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2013/09 - 2017/01 Phospholipid nitroxidation: Development of analytical strategies based on mass spectrometry toward identifying nitro oxidative modifications in phospholipids
Co-supervisor of Tânia Sofia Rodrigues de Melo
 Bioquímica (PhD)
Universidade de Aveiro, Portugal
2015/09 - 2016/12 Validation and application of a targeted metabolomics approach to vineyard samples using UPLC/QqQ-MS/MS
Supervisor of Pedro Santos Garcia Ramos
 Controlo de Qualidade (Master)
Universidade do Porto Faculdade de Farmácia, Portugal
2014/01 - 2016/12 Estudio de la reactividad de óxidos metálicos y óxidos metálicos funcionalizados en los procesos de adsorción de contaminantes emergentes de interés ambiental. Evaluación analítica en técnicas de extracción en fase sólida
Co-supervisor of Valeria Haydée Springer
 Post-Doc, Departamento de Química, Universidad Nacional del Sur, Bahía Blanca, Argentina, grant CONICET (Other)
Universidade do Porto Faculdade de Farmácia, Portugal
2011/09 - 2016/10 Desenvolvimento e validação de métodos rápidos não-invasivos para a determinação de Archaea metanogénica no rúmen
Co-supervisor of Hugo Miguel Rodrigues Cunha Oliveira
 Post-Doc, REQUIMTE/ICBAS, grant FCT SFRH/BPD/75065/2010 (Other)
Universidade do Porto Faculdade de Farmácia, Portugal
2015/07 - 2016/08 HI-Tech - Hybridization mesofluidic techniques for determination of microRNA in biological fluids
Supervisor of Luís Miguel Andrade de Magalhães
 Post-doc, REQUIMTE/FFUP, grant FCT SFRH/BPD/101722/2014 (Other)
Universidade do Porto Faculdade de Farmácia, Portugal
2016/04 - 2016/06 Development and validation of analytical methods based on MS/MS coupled to liquid chromatography applied to biological and environmental samples
Supervisor of Eduarda Marlene Peixoto da Silva
 Post-doc, REQUIMTE/FFUP, grant FCT project UID/MULTI/04378 (Other)
Universidade do Porto Faculdade de Farmácia, Portugal
2011/09 - 2015/04 Skin and seed grape extract as an antioxidant for mechanically deboned chicken meat, during frozen storage
Co-supervisor of Hernan Horácio Tournour
 Ciências do Consumo Alimentar e Nutrição (PhD)
Universidade do Porto Faculdade de Ciências da Nutrição e Alimentação, Portugal
2013/03 - 2014/12 Development and application of environmentally friendly analytical methods for evaluation and monitoring of emerging pollutants
Co-supervisor of Marcelo Gil Machado Vaz Osório
 Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2008/09 - 2012/12 Desenvolvimento de métodos baseados em sistemas biomiméticos ou biológicos para avaliação de propriedades antioxidantes
Supervisor of Carla Sandra Mota Castro
 Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2011/09 - 2012/11 Preparação de materiais de impressão molecular para fármacos e a sua aplicação em técnicas de extração miniatura Lab-on-a-Valve
Co-supervisor of Maria Teresa Barroso Regueiras Silva Carvalho
 Química
Universidade do Porto Faculdade de Ciências, Portugal
2006/09 - 2010/12 Desenvolvimento de métodos analíticos automáticos para determinação da capacidade antioxidante visando espécies com relevância biológica
Co-supervisor of Joana Patrícia Niz Ribeiro
 Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2006/09 - 2010/12 Metodologias automáticas para o processamento não-poluente de amostras e acoplamento a cromatografia líquida
Supervisor of Hugo Miguel Rodrigues Cunha Oliveira
 Ciências Farmacêuticas (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2009/09 - 2010/11 Automatização do processo de extracção em fase sólida para a determinação de compostos fenólicos em alimentos
Supervisor of Sara Cristina Magalhães Monteiro
 Controlo de Qualidade (Master)
Universidade do Porto Faculdade de Farmácia, Portugal
2004/09 - 2008/03 Desenvolvimento de métodos analíticos automáticos baseados em técnicas de fluxo contínuo para avaliação de propriedades antioxidantes em produtos farmacêuticos e em alimentos
Supervisor of Luís Miguel Andrade de Magalhães
 Química Analítica (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2005/09 - 2006/12 Metodologias automáticas para controlo de qualidade de formulações de alopurinol baseadas em biocatalisadores
Co-supervisor of Cristina Isabel da Cunha Silvestre
 Controlo de Qualidade (Master)
Universidade do Porto Faculdade de Farmácia, Portugal
2004/09 - 2005/12 Determinação de ião bromato em águas através de um sistema automático baseado em multi-seringa
Supervisor of Liliana Sara Melo Oliveira
 Química Analítica Ambiental (Master)
Universidade do Porto Faculdade de Farmácia, Portugal
2004/03 - 2005/01 Desenvolvimento de uma metodologia automática de fluxo baseada em multi-seringa com pré-concentração em linha para a determinação de compostos fenólicos
Supervisor of Hugo Miguel Rodrigues Cunha Oliveira
 Química Analítica Ambiental (Master)
Universidade do Porto Faculdade de Farmácia, Portugal
2003/09 - 2004/12 Desenvolvimento de uma metodologia automática de fluxo contínuo para a determinação de compostos fenólicos em meios micelares
Supervisor of Ana Mafalda Lopes Coelho
 Química Analítica Ambiental (Master)
Universidade do Porto Faculdade de Farmácia, Portugal
2003/09 - 2004/10 Sistemas baseados em multi-seringa para a determinação de micronutrientes em extractos de solo
Supervisor of Delfina Maria Coelho Gomes Vasconcelos
 Química Analítica Ambiental (Master)
Universidade do Porto Faculdade de Farmácia, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2022/11/08 Desarrollo y evaluación de metodologías de microextracción para el análisis de volúmenes pequeños de muestra
Thesis Member
 José Grau Escribano (PhD)
Universitat de València Departament de Química Analítica, Spain
2021/07/02 Aplicació de nous materials i formats amb propietats millorades per a lanàlisi de drogues
Thesis Member
 Aitor Sorribes Soriano (PhD)
Universitat de València Departament de Química Analítica, Spain
2021/06/28 Phaseolus vulgaris L. A contribution for the valorization of Portuguese varieties
Thesis Member
 Elsa Velez Mecha (PhD)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2021/04/12 Multi-layer carbon cartridges for determination of EU multi-class organic micropollutants
(Thesis) Main arguer
 Marta Sofia Oliveira Barbosa (PhD)
Universidade do Porto Faculdade de Engenharia, Portugal
2020/11/18 Electromembrane extraction - enhanced theoretical understanding and new applications
(Thesis) Main arguer
 Magnus Saed Restan (PhD)
Universitetet i Oslo, Norway
2017/12/14 Desarrollo de metodologias analíticas para la caracterización química de aceite de oliva virgen
(Thesis) Main arguer
 Felipe Jesús Lara Ortega (PhD)
Universidad de Jaén, Spain
2015/11/20 Estudio de disruptores endocrinos en el medio ambiente
(Thesis) Main arguer
 Noelia Salgueiro González (PhD)
Universidade da Coruña, Spain
2011/12/15 Técnicas luminescentes en fase sólida acopladas a sistemas en flujo continuo. Aplicaciones.
(Thesis) Main arguer
 Silvia Ortega Algar (PhD)
Universidad de Jaén, Spain
2011/09/22 Desarollo e estúdio comparativo de nuevos métodos automáticos de extracción dinâmica para la determinación de elementos traza en muestras sólidas medioambientales
(Thesis) Main arguer
 María Emilia Rosende Mustillo (PhD)
Universitat de les Illes Balears, Spain
2008/11/17 Development of automated methods using syringe based flow analysis techniques and capillary electrophoresis for biotechnological process monitoring and environmental analysis
(Thesis) Main arguer
 Burkhard Horstkotte (PhD)
Universitat de les Illes Balears, Spain
2006/12/18 Métodos de análisis en flujo para la determinación y especiación de elementos traza de interés ambiental
(Thesis) Main arguer
 Luz Olívia Leal Quezada (PhD)
Universitat de les Illes Balears, Spain

Association member

Society Organization name Role
2007 - Current Portuguese Biochemistry Society (Sociedade Portuguesa de Bioquímica) Member
2004 - Current IUPAC – International Union of Pure and Applied Chemistry member
2001 - Current Portuguese Chemical Society (Sociedade Portuguesa de Química) Sócio efetivo

Committee member

Activity description
Role 		Institution / Organization
2018/06 - Current Member of the Steering Committee of the Division of Analytical Chemistry - European Chemical Society (EuChemS)
Member
 EuChemS European Chemical Society, Belgium
2020/03 - 2023/03 Vice-leader of WG4 - Sample Treatment and Microfluidic in COST Action CA 16215 PortASAP - European network for the promotion of portable, affordable and simple analytical platforms
Coordinator
 European Cooperation in Science and Technology, Belgium

Conference scientific committee

Conference name Conference host
2021 - 2023 25th International Symposium on Advances in Extraction Technologies (ExTech 2023), Tenerife Tenerife, Espanha
2021 - 2022/12 12º Encontro Nacional de Cromatografia, Aveiro
2021 - 2022/06 Flow Analysis XV, Cracóvia
2021 - 2022/03 2nd European Sample Preparation e-Conference (EuSP2022), online e Creta
2021/06 - 2021/06 XXIII International Symposium on Advances in Extraction Technologies (ExTech 2021), Alicante
2018 - 2019 Euroanalysis 2019, Istanbul Turkish Chemical Society
2018 - 2018 Flow Analysis XIV, Bangkok
2018 - 2018 Polish-Czech Symposium FA & CE 2018- Flow Analysis & Capillary Electrophoresis, Prague
2014 - 2015 Flow Analysis XIII - 13th International Conference on Flow Analysis, Prague
2014 - 2014 IUPAC Workshop on Determining Antioxidants as Reactive Species Scavengers
2012 - 2012 Flow Analysis XII - 12th International Conference on Flow Analysis, Thessaloniki

Evaluation committee

Activity description
Role 		Institution / Organization Funding entity
2023 - Current Research grant evaluator in competitive call - FCT PhD grants, call 2023
Evaluator
 Fundação para a Ciência e a Tecnologia, Portugal
2022/06 - Current Research grant evaluator in competitive call
Evaluator
 Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil
2018 - 2023 Research grant evaluator in competitive call
Evaluator
 Fonds National de la Recherche Scientifique
2014 - 2021 Research grant evaluator in competitive call
Evaluator
 Grantová agentura Ceské republiky
2020/09 - 2020/10 Research grant evaluator in competitive call
Evaluator
 Latvijas Zinatnes Padome
2012 - 2012 Research grant evaluator in competitive call
Evaluator
 Eidgenössische Technische Hochschule Zürich

Mentoring / Tutoring

Topic Student name
2024/01 - Current Chromatographic method for quantification of antifibrinolytic compounds Federica Guidetti
2019 - Current Desenvolvimento de estratégias de fluxo para o acoplamento de cumarinas a fosfolípidos Ricardo de Lima Belinha
2019 - Current Desenvolvimento de uma metodologia de despiste de atividade estrogénica em amostras de água utilizado o ensaio Yeast Estrogen Screen Diego Pettigrosso
2022/11/01 - 2023/04 Advanced training in analytical methods - automatic evaluation of enzyme activity Michelangelo Sacco
2021/11/01 - 2022/04/30 Advanced training in analytical methods - validation of chromatographic method for determination of uremic solutes Stefano Compagnolo
2021/03/01 - 2021/08/31 Development and validation of a liquid chromatography method for determination of L-carnitine and acetyl-L-carnitine in food suplements Giulia Pia Pizzorno
2021/07/01 - 2021/07/31 Evaluation of estrogenic activity using YES assay Llucia García Moll
2020/09/04 - 2021/05/28 Advanced training in analytical methods - validation of chromatographic method for determination of saccharin Martin Durc
2019 - 2021 Despiste da presença de fluoroquinolonas em água através de dispositivos móveis Leonor Pereira Lima
2019 - 2020 Avaliação de metodologias rápidas para determinação da atividade enzimática da beta-galactosidase em aplicações biotecnológicas Aline Hsiao Mei Cheong
2018 - 2020 Desenvolvimento de sondas do tipo cumarina-iTRAQ para conjugação com estruturas biológicas Inês Dias Baía
2018/09 - 2019/07/25 Recent advances on the synthesis and application of glycerophospholipid fluorescent molecular probes Pedro António Martins Mira Varandas
2018/09 - 2019/07/19 Flow-based dynamic approach to assess bioaccessible zinc in solid pet food samples Bruno José Rodrigues Gregório
2018/09/24 - 2019/02/13 Antibiotic detection in environmental waters using mobile phones Antonina Konarzewska
2018/09 - 2019/01 Determination of antibiotics in water using digital image colorimetry Ana Rosa Moreira da Silva
2018/10/01 - 2018/11/30 Estudo da toxicidade de poluentes emergentes em culturas celulares Miquel Oliver Rodríguez
2017/09 - 2018/03 Chromatographic method for the simultaneous quantification of dapsone and clofazimine in nanoformulations Sandia Santos Machado
2017/09 - 2018 Determination of tranexamic acid in human plasma by UHPLC coupled with tandem mass spectrometry targeting sub-microgram per milliliter levels Sara Regina Rodrigues Fernandes
2017 - 2018 Implementação de uma metodologia para determinação de imunoglobulinas por espectrometria de massa em tandem Gabriela dos Santos Guerra
2017 - 2018 Desenvolvimento de métodos cromatográficos para aplicações biológicas Eduardo Filipe Teixeira Gouveia Sendas Moreira
2017/07/03 - 2017/07/31 Aplicação e validação de um método baseado em sondas imunoquímicas para a determinação de poluentes emergentes Peter Carl
2016/09 - 2017/07 Determinação de zinco em amostras alimentares Rute Isabel Correia Martins
2016 - 2017 Desenvolvimento e validação de uma metodologia cromatográfica para determinação de metotrexato em nanopartículas Patricia Raquel Raimundo de Sousa
2016 - 2017 Desenvolvimento e validação de uma metodologia cromatográfica para determinação de ácido tranexâmico em amostras biológicas Ana Júlia Leiria Amoreira
2015/09 - 2016/12 Metabolomics in wine analysis: state-of-the art Pedro Santos Garcia Ramos
2016/06/01 - 2016/07/31 Desenvolvimento de métodos baseados em técnicas separativas para a avaliação de poluentes emergentes Svetlana Djogo Mracevic
2015 - 2016 Desenvolvimento e validação de uma metodologia analítica para avaliação da captação do radical peroxilo baseada na sonda espectrofotométrica piranina Bruno José Rodrigues Gregório
2015 - 2016 Utilização da fluoresceína como sonda de oxidação em ensaios de avaliação antioxidante: aspetos metodológicos Joana Rodrigues Braz de Carvalho
2014/09 - 2015/07 Programmable flow system for automation of ORAC-pyrogallol red methodology towards estimation of antioxidant reactivity Inês Isabel Barros Moreira Ramos
2013/09/15 - 2013/12/22 Desenvolvimento de um sistema automático para a determinação de cotinina em saliva Adlin Ramdzan
2012/09/10 - 2013/07/31 Determinação da capacidade de méis oriundos de diferentes zonas mediterrânicas Ana Claudia Monteiro Braga
2012/09 - 2013/07 Analytical features of diclofenac as a potential marker of anthropogenic pollution Marcelo Gil Machado Vaz Osório
2012 - 2013 Avaliação do efeito protetor das drogas cardiovasculares sobre a oxidação de proteica mediada pelas espécies reativas do oxigénio Ana Isabel Pimenta da Mota
2012 - 2013 Desenvolvimento e aplicação de um método espectrofotométrico com elevada sensibilidade para a avaliação de antioxidantes através da capacidade de redução do cobre (II) Sara Cristina da Silva Marques
2012 - 2013 Desenvolvimento de uma metodologia de fluxo para a avaliação da capacidade antioxidante de amostras alimentares sólidas Tânia Raquel Pereira Soares
2012/01/23 - 2012/06/23 Desenvolvimento de uma metodologia para extração dinâmica de antioxidantes presentes em alimentos Barbara Korsak
2012/03/01 - 2012/05/31 Development of a fast response and sensitive antioxidant assay based on flow injection analysis Matylda Partyka
2012/03/01 - 2012/05/31 Avaliação das propriedades antioxidants de resíduos de café Joanna Zofia Kwiatkowska
2011 - 2012 Desenvolvimento de uma metodologia de fluxo para a avaliação da capacidade antioxidante de amostras sólidas Miguel Araújo Maia
2010 - 2011 Desenvolvimento de uma metodologia baseada em polímeros de impressão molecular para a determinação de riboflavina em leites Marcelo Gil Machado Vaz Osório
2010/07/12 - 2010/08/09 Desenvolvimento de estratégias para imobilização de biomoléculas em colunas monolíticas Mário Almir Feres Júnior
2009/09 - 2010/07 Métodos automáticos baseados em técnicas de fluxo acoplados à determinação cromatográfica para a preparação em linha de amostras Sara Cristina Magalhães Monteiro
2010/02/01 - 2010/05/01 Desenvolvimento de um sistema automático para a pré-concentração de crómio (VI) utilizando adsorventes nano-estruturados Maria Emília Rosende Mustillo
2009/09/15 - 2009/12/15 Desenvolvimento de uma metodologia automática para pré-concentração e determinação de bromato em águas para consumo humano Liliana Sara Melo Oliveira
2009/05/11 - 2009/08/11 Desenvolvimento de um sistema automático para a manipulação de adsorventes nano-estruturados em sistemas de fluxo Warunya Bonjoob
2008/10/24 - 2009/06/20 Desenvolvimento de sistemas automáticos baseados em detecção por quimioluminescência para determinações em amostras biológicas e ambientais Mortaza Iranifam
2007 - 2008 Avaliação comparativa de metodologias colorimétricas baseadas na análise por injeção em fluxo para a determinação de ferro em amostras ambientais Elisa Pinto Freitas
2007 - 2008 Avaliação comparativa de metodologias colorimétricas baseadas na análise por injeção em fluxo para a determinação de ferro em amostras ambientais Filipa Alexandra de Carvalho Benites Dias
2006 - 2007 Desenvolvimento de um método rápido para a determinação de compostos fenólicos totais usando a reação de Folin-Ciocalteu Fernando Miguel Moreira Santos
2005/09 - 2006/11 Utilização de enzimas em sistemas SIA: estado da arte e perspectivas Cristina Isabel da Cunha Silvestre
2005/05/01 - 2006/03/31 Desenvolvimento de um sistema automático para a determinação de nitrofenóis baseado recorrendo a um óptrodo e calibração multivariada Matias Manera Fuentes
2005/06/15 - 2005/08/31 Desenvolvimento de uma metodologia automática para acoplamento com técnicas de atomização por chama Maria Inês Gameiro de Sá Almeida
2003/11/01 - 2003/12/31 Desenvolvimento de um sistema de fluxo para a determinação de diidroxiacetona através da inibição da enzima glicerol desidrogenase Viviane Grassi
2000 - 2001 Desenvolvimento de um sistema de análise por injeção sequencial para a determinação de sulfatos em vinhos Helena I. O. R. Silva
Distinctions

Award

2016 FIA Award for Science
Japanese Association for Flow Injection Analysis, Japan
2015 Award for Entrepreneurship (Prémio Empreendedorismo e Inovação Crédito Agrícola)
Crédito Agrícola, Portugal
2015 Award for Entrepreneurship (concurso INOVPORTUGAL - categoria de Novas Ideias)
Associação Acredita Portugal, Portugal

Other distinction

2023 Cover feature at Analytical Chemistry journal
American Chemical Society, United States
2023 Best oral presentation award at 12º Encontro Nacional de Cromatografia
Sociedade Portuguesa de Química, Portugal
2021 Cover feature at Analytical Chimica Acta journal
Elsevier BV, Netherlands
2020 Cover feature at Bioconjugate Chemistry journal
American Chemical Society, United States
2020 Female Role Model in Analytical Chemistry
Springer Nature Deutschland GmbH Heidelberg, Germany
2019 Best poster award at 11º Encontro Nacional de Cromatografia
Springer - Analytical & Bioanalytical Chemistry Journal, Germany
2019 Best oral presentation at Euroanalysis 2019
Elsevier BV, Netherlands
2018 Cover feature at Analytical Methods journal
Royal Society of Chemistry, United Kingdom
2014 Cover feature at Analytica Chimica Acta journal
Elsevier BV, Netherlands
2014 Cover feature at Analytical Methods journal
Royal Society of Chemistry, United Kingdom
2010 Cover feature at Luminescence journal
John Wiley & Sons Inc, United States