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Daniel Simão is a Lab Head at the Animal Cell Technology Unit of IBET's Health & Pharma Division. D. Simão graduated in Biology (University of Lisbon) and holds an MSc in Molecular Genetics and Biomedicine (NOVA School of Science and Technology). Obtained his PhD in Bioengineering Systems from the MIT-Portugal Doctoral Program in 2016. Having pursued his PhD studies in the Advanced Cell Models Laboratory (iBET and ITQB NOVA), D. Simão focused mostly on the development of stem cell-based 3D in vitro models of central nervous system for preclinical research. His work contributed for the establishment of neural differentiation methodologies based on stirred culture systems, interrogating functional aspects of neuron-glia interactions and applying such models for evaluation of viral vectors for gene therapy. Joined the Adenovirus Receptors, Trafficking and Vectorology Laboratory at IGMM (Montpellier, France) for a post-doc, working on the construction and production of CAV-2 vectors for gene transfer to central nervous system. In 2017, Daniel became Senior Researcher at iBET responsible for the development of a microfluidics-based antibody discovery platform. As Lab Head since 2021, Daniel coordinates a team working on R&D projects for biopharmaceutical industry, focusing on biologics research. D. Simão has authored 18 manuscripts, 3 conference papers and 1 book chapter; Has received 5 awards and/or honors and participated as speaker in 11 national and international conferences. Daniel has been responsible for the co-supervision of one PhD Student and has participated as invited lecturer in Master and PhD programs (NOVA Medical School and ITQB/iBET). Was the Principal Investigator of the FCT funded project AstroReact and the internally-funded (iBET) project FibroTag, in the fields of development of human cell models for preclinical research and antibody discovery; Participated as team member in several other publicly funded research projects (FCT and EC), and operates as project manager in research services with the biopharmaceutical industry.
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Identification

Personal identification

Full name
DANIEL FILIPE MESTRE SIMÃO

Citation names

  • Simão, Daniel

Author identifiers

Ciência ID
0A15-08E9-448D
ORCID iD
0000-0002-7532-8081
Researcher Id
AAF-2747-2021

Addresses

  • Avenida da República, Estação Agronómica Nacional, Edifício iBET/ITQB-NOVA, 2780-157, Oeiras, Oeiras, Portugal (Professional)

Websites

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism
  • Natural sciences - Biological Sciences - Cell Biology
  • Natural sciences - Biological Sciences - Molecular Biology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2)
Spanish; Castilian Beginner (A1) Intermediate (B1) Beginner (A1) Beginner (A1)
Education
Degree Classification
2016
Concluded
 Bioengineering Systems (MIT-Portugal) (Doutoramento)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Development of human central nervous system 3D in vitro models for preclinical research" (THESIS/DISSERTATION)
 Aprovado, por unanimidade
2010
Concluded
 Mestrado em Genética Molecular e Biomedicina (Mestrado)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Novas Linhas Celulares Humanas para Produção de Adenovírus com Aplicação em Terapia Génica" (THESIS/DISSERTATION)
 18
2008
Concluded
 Biologia - Ramo de Biologia Molecular e Genética (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
 15
Affiliation

Science

Category
Host institution 		Employer
2021/02/01 - Current Principal Investigator (Research) Instituto de Biologia Experimental e Tecnológica, Portugal
Instituto de Biologia Experimental e Tecnológica, Portugal
2017/07/01 - 2021/01/31 Contracted Researcher (Research) Instituto de Biologia Experimental e Tecnológica, Portugal
2016/09/01 - 2017/06/30 Postdoc (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2016/04/01 - 2016/08/31 Contracted Researcher (Research) Institut de Génétique Moléculaire Montpellier, France
2016/01/01 - 2016/03/31 Researcher (Research) Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2012/01/01 - 2015/12/31 Researcher (Research) Fundação para a Ciência e a Tecnologia, Portugal
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2010/03/16 - 2011/09/30 Research Assistant (Research) Instituto de Biologia Experimental e Tecnológica, Portugal

Others

Category
Host institution 		Employer
2014/01 - 2014/02 Visiting PhD Student Norges Teknisk-Naturvitenskapelige Universitet Fakultet for medisin og helsevitenskap, Norway
Projects

Grant

Designation Funders
2019/01/01 - 2019/10/31 iNOVA4Health - Programme in Translational Medicine (iBET, CEDOC/FCM, IPOLFG and ITQB)
UID/Multi/04462/2019
Instituto Português de Oncologia de Lisboa Francisco Gentil EPE, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal

Universidade Nova de Lisboa, Portugal

Universidade Nova de Lisboa Centro de Estudos de Doenças Crónicas, Portugal

Universidade Nova de Lisboa Medical School, Portugal

Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2023/09/01 - Current Life Sciences for a Healthy and Sustainable Future
LA/P/0087/2020
10.54499/LA/P/0087/2020
Instituto Português de Oncologia de Lisboa Francisco Gentil EPE, Portugal

Universidade Nova de Lisboa Molecular Structural and Cellular Microbiology, Portugal

Universidade Nova de Lisboa, Portugal

Instituto de Biologia Experimental e Tecnológica, Portugal

Instituto Gulbenkian de Ciência, Portugal

Universidade de Lisboa Centro de Estudos Comparatistas, Portugal

BioResources 4 Sustainability, Portugal

Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2020/06/01 - 2022/05/31 Novel antibody-based tools for targeting cardiac fibrosis
iBETxplore_2019_738
Principal investigator
Instituto de Biologia Experimental e Tecnológica, Portugal
 Instituto de Biologia Experimental e Tecnológica
Ongoing
2018/10/01 - 2021/09/30 Impacto das alterações do microambiente neural induzidas por astrócitos na patobiologia de lesões traumáticas cerebrais
PTDC/BTM-ORG/29580/2017
Principal investigator
Instituto de Biologia Experimental e Tecnológica, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2014/12/01 - 2018/05/31 CARDIOSTEM: Tecidos cardíacos e terapias baseadas em células estaminais para aplicações cardiovasculares
MITP-TB/ECE/0013/2013
Research Fellow
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal

Centro Hospitalar de Lisboa Central EPE, Portugal

Instituto de Biologia Experimental e Tecnológica, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2012/03 - 2015/08 Human central nervous system in vitro models for preclinical research: new tools for studying viral vector-mediated gene delivery in 3D cell context
Provided by PTCRIS: 119243
Research Fellow
Fundação para a Ciência e a Tecnologia, I.P.
2014/04/01 - 2015/03/31 Microscopia microfluídica de "light-sheet" para characterização de modelos celulares tridimensionais e peixa-zebra
EXPL/BBB-IMG/0363/2013
Fundação Champalimaud, Portugal

Fundação Calouste Gulbenkian, Portugal

Instituto Gulbenkian de Ciência, Portugal

Instituto de Biologia Experimental e Tecnológica, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2014/04 - 2015/03 Microfluidics light-sheet microscopy for increased throughput analysis of 3D cell models and zebra-fish
Provided by PTCRIS: 133385
Research Fellow
Fundação para a Ciência e a Tecnologia, I.P.
Outputs

Publications

Book chapter
  1. Simão D; Arez F; Terasso AP; Pinto C; Sousa MF; Brito C; Alves PM. "Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells.". In Bioreactors in Stem Cell Biology. New York, United States: Humana Press, 2016.
    Published • 10.1007/7651_2016_333
Conference paper
  1. Simão, Daniel. "Development of an anti-HER2 phage to monitor untargeted cell pannings". Paper presented in 26th European Society for Animal Cell Technology Meeting - Cell culture technologies: bridging academia and industry to provide solutions for patients, 2019.
  2. Simão D; Pinto C; Teixeira AP; Alves PM; Brito C. "1H-NMR spectroscopy for human 3D neural stem cell cultures metabolic profiling.". Paper presented in 3rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health, 2013.
    10.1186/1753-6561-7-s6-o8
  3. Simão D; Costa I; Serra M; Schwarz J; Brito C; Alves PM. "Towards human central nervous system in vitro models for preclinical research: strategies for 3D neural cell culture.". Paper presented in 22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies, 2011.
    10.1186/1753-6561-5-S8-P53
Journal article
  1. Catarina M. Gomes; Maria João Sebastião; Gabriela Silva; Filipa Moura; Daniel Simão; Patrícia Gomes-Alves; Paula M. Alves; Catarina Brito. "Miniaturization of hiPSC-derived 3D neural cultures in stirred-tank bioreactors for parallelized preclinical assessment of rAAV". Frontiers in Bioengineering and Biotechnology (2024): http://dx.doi.org/10.3389/fbioe.2024.1379597.
    10.3389/fbioe.2024.1379597
  2. Inês C. Ferreira; Estefania Torrejón; Bernardo Abecasis; Bruno M. Alexandre; Ricardo A. Gomes; Chris Verslype; Jos van Pelt; et al. "Aldehyde Dehydrogenase 2 (ALDH2): A novel sorafenib target in hepatocellular carcinoma unraveled by the proteome-wide cellular thermal shift assay". SLAS Discovery (2024): https://doi.org/10.1016/j.slasd.2024.100154.
    10.1016/j.slasd.2024.100154
  3. Gabriela Silva; Ana F. Rodrigues; Susana Ferreira; Carolina Matos; Rute P. Eleutério; Gonçalo Marques; Khrystyna Kucheryava; et al. "Novel scFv against Notch Ligand JAG1 Suitable for Development of Cell Therapies toward JAG1-Positive Tumors". Biomolecules (2023): https://www.mdpi.com/2218-273X/13/3/459.
    10.3390/biom13030459
  4. Moita MR; Silva MM; Diniz C; Serra M; Hoet RM; Barbas A; Simão D. "Transcriptome and proteome profiling of activated cardiac fibroblasts supports target prioritization in cardiac fibrosis.". Frontiers in cardiovascular medicine (2022): http://europepmc.org/abstract/med/36531712.
    10.3389/fcvm.2022.1015473
  5. Simão D; Gomes CM; Alves PM; Brito C. "Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models.". Biotechnology advances (2021): http://europepmc.org/abstract/med/34875362.
    10.1016/j.biotechadv.2021.107883
  6. Vasconcelos E Sá J; Simão D; Terrasso AP; Silva MM; Brito C; Isidro IA; Alves PM; Carrondo MJT. "Unveiling dynamic metabolic signatures in human induced pluripotent and neural stem cells.". PLoS computational biology (2020): http://europepmc.org/abstract/med/32298259.
    10.1371/journal.pcbi.1007780
  7. Arez F; Rebelo SP; Fontinha D; Simão D; Martins TR; Machado M; Fischli C; et al. "Flexible 3D Cell-Based Platforms for the Discovery and Profiling of Novel Drugs Targeting Plasmodium Hepatic Infection.". ACS infectious diseases (2019): http://europepmc.org/abstract/med/31479238.
    10.1021/acsinfecdis.9b00144
  8. Bayó-Puxan N; Terrasso AP; Creyssels S; Simão D; Begon-Pescia C; Lavigne M; Salinas S; et al. "Lysosomal and network alterations in human mucopolysaccharidosis type VII iPSC-derived neurons.". Scientific reports (2018): http://europepmc.org/abstract/med/30413728.
    10.1038/s41598-018-34523-3
  9. Silva MM; Gomes-Alves P; Rosa S; Simão D; Inácio JM; Peixoto C; Serra M; Belo JA; Alves PM. "Full-length human CCBE1 production and purification: leveraging bioprocess development for high quality glycosylation attributes and functionality.". Journal of biotechnology (2018): http://europepmc.org/abstract/med/30165116.
    10.1016/j.jbiotec.2018.08.015
  10. Simão D; Silva MM; Terrasso AP; Arez F; Sousa MFQ; Mehrjardi NZ; Šaric T; et al. "Recapitulation of Human Neural Microenvironment Signatures in iPSC-Derived NPC 3D Differentiation.". Stem cell reports (2018): http://europepmc.org/abstract/med/30057262.
    10.1016/j.stemcr.2018.06.020
  11. Simão D; Pinto C; Fernandes P; Peddie CJ; Piersanti S; Collinson LM; Salinas S; et al. "Evaluation of helper-dependent canine adenovirus vectors in a 3D human CNS model.". (2016): http://europepmc.org/abstract/med/26181626.
    10.1038/gt.2015.75
  12. Simão D; Terrasso AP; Teixeira AP; Brito C; Sonnewald U; Alves PM. "Functional metabolic interactions of human neuron-astrocyte 3D in vitro networks.". (2016): http://europepmc.org/abstract/med/27619889.
    10.1038/srep33285
  13. Silva AC; Simão D; Küppers C; Lucas T; Sousa MF; Cruz P; Carrondo MJ; Kochanek S; Alves PM. "Human amniocyte-derived cells are a promising cell host for adenoviral vector production under serum-free conditions.". (2015): http://europepmc.org/abstract/med/25943527.
    10.1002/biot.201400765
  14. Simão D; Pinto C; Piersanti S; Weston A; Peddie CJ; Bastos AE; Licursi V; et al. "Modeling human neural functionality in vitro: three-dimensional culture for dopaminergic differentiation.". (2015): http://europepmc.org/abstract/med/25257211.
    10.1089/ten.tea.2014.0079
  15. Fernandes P; Simão D; Guerreiro MR; Kremer EJ; Coroadinha AS; Alves PM. "Impact of adenovirus life cycle progression on the generation of canine helper-dependent vectors.". (2015): http://europepmc.org/abstract/med/25338917.
    10.1038/gt.2014.92
  16. Piersanti S; Burla R; Licursi V; Brito C; La Torre M; Alves PM; Simao D; et al. "Transcriptional Response of Human Neurospheres to Helper-Dependent CAV-2 Vectors Involves the Modulation of DNA Damage Response, Microtubule and Centromere Gene Groups.". (2015): http://europepmc.org/abstract/med/26207738.
    10.1371/journal.pone.0133607
  17. Gualda EJ; Simão D; Pinto C; Alves PM; Brito C. "Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy.". (2014): http://europepmc.org/abstract/med/25161607.
    10.3389/fncel.2014.00221
  18. Brito C; Simão D; Costa I; Malpique R; Pereira CI; Fernandes P; Serra M; et al. "3D cultures of human neural progenitor cells: dopaminergic differentiation and genetic modification. [corrected].". (2012): http://europepmc.org/abstract/med/22433395.
    10.1016/j.ymeth.2012.03.005
  19. Brito, C.; Simão, D.; Costa, I.; Malpique, R.; Pereira, C.I.; Fernandes, P.; Serra, M.; et al. "Corrigendum to " Generation and genetic modification of 3D cultures of human dopaminergic neurons derived from neural progenitor cells" [Methods 56 (2012) 452-460]". Methods 57 1 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84865089501&partnerID=MN8TOARS.
    10.1016/j.ymeth.2012.07.014
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023 In Vitro Tools to Explore Cardiac Fibrosis Target Space Antibody Engineering & Therapeutics Europe
(Amsterdam, Netherlands)
2021/03/08 Human Stem Cells in Disease Modelling and Drug Discovery VI Semana da Bioengenharia
Instituto Superior Técnico (Lisbon, Portugal)
2019/04/19 Mimicking disease microenvironments in Bioreactors 3D Cell Cultures and Organ-on-a-Chip in the Pharmaceutical Toxicology and Pathology - Use and Applicability
Dutch Society of Toxicology (NVT) (Leiden, Netherlands)
2019/04/05 Recapitulation of Human Neural Microenvironment Signatures in iPSC-derived NPC 3D Differentiation Advancing Biologics Characterization by Mass Spectrometry Seminar
iBET/ITQB and Sciex (Oeiras, Portugal)
2018/11/29 Metabolic profiling of human neural cells for disclosing metabolic determinants and cell-cell interactions World Preclinical Congress 2018
WPC Europe (Lisbon, Portugal)
2018/06/05 iPSC-derived neurospheroids recapitulate development and pathological signatures of brain microenvironment 3D Cell Culture 2018
DECHEMA (Freiburg, Germany)
2017/10/18 Dynamic remodelling of neural cellular and extracellular signatures depicted in 3D in vitro differentiation of human iPSC-derived NSC 25th Anniversary Congress of the European Society for Gene and Cell Therapy
ESGCT (Berlin, Germany)
2017/05/14 Dynamic remodeling of neural cellular and extracellular signatures depicted in 3D in vitro differentiation of human iPSC-derived NSC 25th European Society for Animal Cell Technology meeting
ESACT (Lausanne, Switzerland)
2016/10/18 Towards improved predictability in pre-clinical research: Human 3D neural in vitro model for assessment of gene therapy vectors 19th International Congress ESTIV - European Society of Toxicology In Vitro
ESTIV (Juan-les-Pins, France)
2015/06/01 Metabolic profiling of human neural cells: combining 3D in vitro models and 13C-NMR Spectroscopy 24th European Society for Animal Cell Technology meeting
ESACT (Barcelona, Spain)
2015/03/11 Towards improved predictability in pre-clinical research: Human 3D neural in vitro model for assessment of gene therapy vectors 5th Spring Meeting
ISBiotech (Washington D.C., United States)
2012/09/20 Novel Strategies for 3D neural culture & gene delivery: towards human CNS in vitro models for preclinical research Bioimaging 2012 - 1st International Symposium in Applied Bioimaging
Instituto de Engenharia Biomédica (INEB) (Porto, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2017/01/01 - Current Phenotypic screening for identification of disease-relevant antigens using phage-display technology
Co-supervisor
 Biociências Moleculares (PhD)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2021/03/05 Live cell biosensors for detection and quantification of viral pathogens
(Thesis) Main arguer
 Ana Raquel Fernandes (Master)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2017 - 2020 Modelos in vitro em biomedicina Medicina (Mestrado integrado) Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal
Distinctions

Award

2016 Best Young Scientist Oral Presentation
European Society of Toxicology In Vitro (ESTIV), Netherlands
2013 Registration Award for top 10 selected abstracts for 8th SPCE-TC International meeting
Sociedade Portuguesa de Células Estaminais e Terapia Celular (SPCE-TC), Portugal
2010 Highest classification in Molecular Genetics and Biomedicine MSc
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Other distinction

2016 Travel grant for attendance in the EMBO|EMBL Symposium: Organoids – Modelling Organ Development and Disease in 3D Culture
European Molecular Biology Laboratory, Germany
2015 Travel grant for attendance in the BIOMS|EMBL conference on Physics of Cells and Tissues 2015 - Modeling meets Experiment
European Society for Animal Cell Technology (ESACT), Germany