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Identification

Personal identification

Full name
Maria João Martinho de Freitas

Citation names

  • FREITAS, MARIA JOÃO

Author identifiers

Ciência ID
0D1F-3491-5270
ORCID iD
0000-0002-7276-0522
Researcher Id
D-3816-2018

Knowledge fields

  • Natural sciences - Biological Sciences - Cell Biology
  • Natural sciences - Biological Sciences - Molecular Biology
  • Social Sciences - Media and Communications

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2) Advanced (C1)
Education
Degree Classification
2020/04/01 - 2020/04/30
Concluded
Coaching for Postdocs (Outros)
Katholieke Universiteit Leuven, Belgium
2019/10/01 - 2019/10/31
Concluded
Writing Skills for Biomedical Researchers (Outros)
Katholieke Universiteit Leuven, Belgium
2018/01/15
Concluded
Biologia (Doutoramento)
Universidade de Aveiro, Portugal
"Addressing sperm motility regulation through characterization and modulation of the GSK3/PPP1R2/PPP1 signaling pathway" (THESIS/DISSERTATION)
2015/11/02 - 2015/11/06
Concluded
Networks and Pathways 2015 (Outros)
European Bioinformatics Institute, United Kingdom
2012/06/06
Concluded
Biomedicina Molecular (Mestrado integrado)
Universidade de Aveiro, Portugal
"Identification of Tctex1d4 interactome in human testis" (THESIS/DISSERTATION)
2010/06/28
Concluded
Ciências Biomédicas (Licenciatura)
Universidade de Aveiro, Portugal
Affiliation

Science

Category
Host institution
Employer
2023/09/25 - Current Science and Technology Management Universidade de Coimbra, Portugal
2018/10/01 - 2022/04/30 Postdoc (Research) Katholieke Universiteit Leuven Departement Cellulaire en Moleculaire Geneeskunde, Belgium
Katholieke Universiteit Leuven Departement Cellulaire en Moleculaire Geneeskunde, Belgium
2018/03/01 - 2018/09/01 Postdoc (Research) Universidade de Aveiro, Portugal
Universidade de Aveiro, Portugal
Projects

Grant

Designation Funders
2013/12/01 - 2017/11/30 ADDRESSING SPERM MOTILITY REGULATION BY SPECIFIC PPP1 INHIBITORS
SFRH/BD/84876/2012
Universidade de Aveiro, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2018/10/01 - 2021/09/01 Selective tyrosine phosphorylation of the PKD2 activation segment: a novel regulatory mechanism to achieve isoenzyme specific signaling within the protein kinase D family.
G0C4118N
Post-doc Fellow
Katholieke Universiteit Leuven, Belgium
Fonds Wetenschappelijk Onderzoek Vlaanderen
Concluded
2019/01/01 - 2019/12/31 Instituto de Biomedicina - Aveiro
UID/BIM/04501/2019
Universidade de Aveiro, Portugal

Universidade de Aveiro Instituto de Biomedicina, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016/07/01 - 2019/12/31 Interacções proteína-proteína como alvos terapêuticos: modulação de complexos da Fosfoproteina Fosfatase 1 na fertilidade masculina
PTDC/BBB-BQB/3804/2014
Universidade de Aveiro, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016/01/01 - 2018/01/15 Protein-protein interactions as drug targets: modulation of Phosphoprotein Phosphatase 1 complexes in male fertility
PTDC/BBB-BQB/3804/2014
Researcher
Universidade de Aveiro, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2013/07/01 - 2015/06/30 Plataforma para o diagnóstico e tratamento da infertilidade masculina baseada em vias de sinalização nos espermatozóides humanos
PTDC/DTP-PIC/0460/2012
Universidade de Aveiro, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Other

Designation Funders
2021/10/01 - 2022/04/30 Deciphering the functional role of PKD2 driver mutations in Multiple Myeloma via an integrated multiomics approach.
00000
Post-doc Fellow
Katholieke Universiteit Leuven, Belgium
Concluded
Outputs

Publications

Book
  1. Joana Vieira Silva; FREITAS, MARIA JOÃO; Fardilha, Margarida. Tissue-Specific Cell Signaling. Springer International Publishing. 2020.
    10.1007/978-3-030-44436-5
Book chapter
  1. Freitas, Maria João; Fardilha, Margarida. "Profiling Signaling Proteins in Sertoli Cells by Co-immunoprecipitation". In Methods in Molecular Biology, 73-84. Springer New York, 2018.
    10.1007/978-1-4939-7698-0_7
  2. Freitas, Maria João; Fardilha, Margarida. "Sertoli cell Preparation Co-immunoprecipitation". In Methods in Molecular Biology, 73-84. Springer New York, 2018.
    10.1007/978-1-4939-7698-0_7
  3. FREITAS, MARIA JOÃO; Daniela Patrício; Fardilha, Margarida. "Sinalização no espermatozoide". 2015.
Conference abstract
  1. Silva, Joana Vieira; FREITAS, MARIA JOÃO; Santiago, Joana; Jones, Sarah; Guimarães, Sofia Cunha; Vijayaraghavan, Srinivasan; Colombo, Giorgio; Howl, John; Fardilha, M.. "A novel approach for male contraception: modulation of phosphoprotein phosphatase I complexes using bioportides". Paper presented in 36th Virtual Annual Meeting of the European-Society-of-Human-Reproduction-and-Embryology (ESHRE), 2020.
    Published
Conference poster
  1. Bárbara Matos; Daniela Patrício; Henriques, Magda Carvalho; Maria João Freitas; Vitorino, Rui; I. F. Duarte; Paula Oliveira; et al. "Chronic exercise training attenuates prostate cancer induced molecular remodelling in the testis". Paper presented in ¿XXI SPB: National Congress of Biochemistry, 2021.
  2. Maria João Freitas; Rudra Kashyap; Johan Van Lint. "PKD2 mutations identified in multiple myeloma cells result in altered PKD2 activity". 2021.
  3. Silva, Joana Vieira; FREITAS, MARIA JOÃO; Santiago, Joana; Jones, Sarah; Howl, John; Fardilha, Margarida. "Modulation of Sperm Motility using bioportides: A novel approach for male contraception". Paper presented in NYRA Meeting 2019, 2019.
  4. Maria João Freitas; Cameron Brothag; Joana Vieira Silva; Fardilha, Margarida; Srinivasan Vijayaraghavan. "Identification and in silico characterization of GSK3 human testis and spermatozoa interactome". Paper presented in From protein structure to biological function through interactomics: an integrated view, 2nd edition, 2018.
  5. Joana Vieira Silva; Maria João Freitas; Bárbara Regadas Correia; António Patrício; Pelech, Steven; Fardilha, Margarida. "Signaling proteins as biomarkers for sperm quality evaluation". Paper presented in 33rd Annual Meeting of the European Society of Human Reproduction and Embryology, 2017.
  6. Daniela Patrício; Maria João Freitas; Sofia Guimarães; Fardilha, Margarida. "Drug screening using Mammalian Two-Hybrid System". Paper presented in 3rd Post-Graduate Symposium on Biomedicine, 2017.
  7. Daniela Patrício; Sofia Guimarães; Maria João Freitas; Fardilha, Margarida. "Targeting protein-protein interaction using Mammalian Two-Hybrid". Paper presented in 8th Meeting on Signal Transduction (SINAL), 2017.
  8. Maria João Freitas; Cameron Brothag; Souvik Dey; Fardilha, Margarida; Srinivasan Vijayaraghavan. "Protein interactors potentially involved in the isoform-specific function of sperm GSK3alpha in male fertility". Paper presented in Society for the study of Reproduction annual meeting 2016, San Diego, USA., 2016.
Journal article
  1. Silva, Joana Vieira; Freitas, Maria João; Santiago, Joana; Jones, Sarah; Guimarães, Sofia; Vijayaraghavan, Srinivasan; Publicover, Steven; et al. "Disruption of protein phosphatase 1 complexes with the use of bioportides as a novel approach to target sperm motility". Fertility and Sterility 115 2 (2021): 348-362. http://dx.doi.org/10.1016/j.fertnstert.2020.08.013.
    10.1016/j.fertnstert.2020.08.013
  2. Gilles, Philippe; Kashyap, Rudra S.; Freitas, Maria João; Ceusters, Sam; Van Asch, Koen; Janssens, Anke; De Jonghe, Steven; et al. "Design, synthesis and biological evaluation of pyrazolo[3,4-d]pyrimidine-based protein kinase D inhibitors". European Journal of Medicinal Chemistry 205 (2020): 112638. http://dx.doi.org/10.1016/j.ejmech.2020.112638.
    10.1016/j.ejmech.2020.112638
  3. Freitas, M J; Silva, J V; Brothag, C; Regadas-Correia, B; Fardilha, M; Vijayaraghavan, S. "Isoform-specific GSK3A activity is negatively correlated with human sperm motility". MHR: Basic science of reproductive medicine 25 4 (2019): 171-183. http://dx.doi.org/10.1093/molehr/gaz009.
    10.1093/molehr/gaz009
  4. Vieira Silva, J.; Freitas, M. J.; Correia, B.; Patricio, A.; Pelech, S.; Fardilha, M.. "Signaling proteins as biomarkers for sperm quality evaluation". Human Reproduction 32 (2017): 154-154. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000416390501128&KeyUID=WOS:000416390501128.
  5. Freitas, M.J.; Vijayaraghavan, S.; Fardilha, M.. "Signaling mechanisms in mammalian sperm motility". Biology of Reproduction 96 1 (2017): 2-12. http://www.scopus.com/inward/record.url?eid=2-s2.0-85022323534&partnerID=MN8TOARS.
    10.1095/biolreprod.116.144337
  6. Silva, J.V.; Cruz, D.; Gomes, M.; Correia, B.R.; Freitas, M.J.; Sousa, L.; Silva, V.; Fardilha, M.. "Study on the short-term effects of increased alcohol and cigarette consumption in healthy young men's seminal quality". Scientific Reports 7 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85017028715&partnerID=MN8TOARS.
    10.1038/srep45457
  7. Bastos, Paulo; Freitas, Maria Joao; Gomes, Andre; Vitorino, Rui; Fardilha, Margarida. "Unravelling the Power of Omics for the Infertile Aging Male". Current Pharmaceutical Design 23 30 (2017): 4451-4469. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000416929300004&KeyUID=WOS:000416929300004.
    10.2174/1381612822666161018155247
  8. Silva, Joana V.; Freitas, Maria Joao; Correia, Barbara R.; Korrodi-Gregorio, Luis; Patricio, Antonio; Pelech, Steven; Fardilha, Margarida. "Signaling fingerprint of the human spermatozoa: correlation with clinical data". Medicine 95 10 (2016): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000375209300103&KeyUID=WOS:000375209300103.
  9. Freitas, Maria Joao; Silva, Joana V.; Felgueiras, Juliana; Patricio, Antonio; Maia, Nuno; Pelech, Steven; Fardilha, Margarida. "Protein Profile in Human Prostate Cancer". Medicine 95 10 (2016): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000375209300178&KeyUID=WOS:000375209300178.
  10. Freitas, Maria Joao; Silva, Joana Vieira; Korrodi-Gregorio, Luis; Fardilha, Margarida. "Non-Stop Lab Week: A Real Laboratory Experience for Life Sciences Postgraduate Courses". Biochemistry and Molecular Biology Education 44 3 (2016): 297-303. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000380265400010&KeyUID=WOS:000380265400010.
    10.1002/bmb.20947
  11. Silva, J.V.; Freitas, M.J.; Correia, B.R.; Korrodi-Gregório, L.; Patrício, A.; Pelech, S.; Fardilha, M.. "Profiling signaling proteins in human spermatozoa: Biomarker identification for sperm quality evaluation". Fertility and Sterility 104 4 (2015): 845-856e8. http://www.scopus.com/inward/record.url?eid=2-s2.0-84942817659&partnerID=MN8TOARS.
    10.1016/j.fertnstert.2015.06.039
  12. Silva, J.V.; Freitas, M.J.; Felgueiras, J.; Fardilha, M.. "The power of the yeast two-hybrid system in the identification of novel drug targets: Building and modulating PPP1 interactomes". Expert Review of Proteomics 12 2 (2015): 147-158. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925301188&partnerID=MN8TOARS.
    10.1586/14789450.2015.1024226
  13. Gomes, M.; Freitas, M.J.; Fardilha, M.. "Physical Activity, Exercise, and Mammalian Testis Function: Emerging Preclinical Protein Biomarker and Integrative Biology Insights". OMICS A Journal of Integrative Biology 19 9 (2015): 499-511. http://www.scopus.com/inward/record.url?eid=2-s2.0-84941043263&partnerID=MN8TOARS.
    10.1089/omi.2015.0065
  14. Cruz, Daniel Filipe; Freitas, Maria João; Patrício, António; Fardilha, Margarida. "Vias de sinalização reguladoras das funções do espermatozoide". Revista Internacional de Andrología 12 3 (2014): 104-111. http://dx.doi.org/10.1016/j.androl.2014.04.006.
    10.1016/j.androl.2014.04.006
  15. Freitas, Maria João; Fardilha, Margarida. "Intraflagellar Protein 88 Interactome Analysis: A Bioinformatics Approach Highlights Its Role in Testis and Sperm Function". Journal of Biophysical Chemistry 05 03 (2014): 99-106. http://dx.doi.org/10.4236/jbpc.2014.53011.
    10.4236/jbpc.2014.53011
  16. Silva, J.V.; Freitas, M.J.; Fardilha, M.. "Phosphoprotein phosphatase 1 complexes in spermatogenesis". Current Molecular Pharmacology 7 2 (2014): 136-146. http://www.scopus.com/inward/record.url?eid=2-s2.0-84930979437&partnerID=MN8TOARS.
  17. Freitas, M.J.; Korrodi-Gregório, L.; Morais-Santos, F.; Da Cruz E Silva, E.; Fardilha, M.. "TCTEX1D4 interactome in human testis: Unraveling the function of dynein light Chain in spermatozoa". OMICS A Journal of Integrative Biology 18 4 (2014): 242-253. http://www.scopus.com/inward/record.url?eid=2-s2.0-84897506850&partnerID=MN8TOARS.
    10.1089/omi.2013.0133
  18. Korrodi-Gregório, L.; Silva, J.V.; Santos-Sousa, L.; Freitas, M.J.; Felgueiras, J.; Fardilha, M.. "TGF-ß cascade regulation by PPP1 and its interactors -impact on prostate cancer development and therapy". Journal of Cellular and Molecular Medicine 18 4 (2014): 555-567. http://www.scopus.com/inward/record.url?eid=2-s2.0-84897964073&partnerID=MN8TOARS.
    10.1111/jcmm.12266
  19. Korrodi-Gregório, L.; Vieira, S.I.; Esteves, S.L.C.; Silva, J.V.; Freitas, M.J.; Brauns, A.-K.; Luers, G.; et al. "TCTEX1D4, a novel protein phosphatase 1 interactor: Connecting the phosphatase to the microtubule network". Biology Open 2 5 (2013): 453-465. http://www.scopus.com/inward/record.url?eid=2-s2.0-84974652624&partnerID=MN8TOARS.
    10.1242/bio.20131065
  20. Freitas, M.J.; Korrodi-Gregório, L.; Esteves, S.; Fardilha, M.. "Identification of therapeutic and diagnotic targets through yeast two hybrid system: Molecular biology in medicine | Identificação de alvos terapêuticos e de diagnóstico através do yeast two hybrid system: A biologia molecular na medicina". Acta Medica Portuguesa 25 6 (2012): 427-432. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873514438&partnerID=MN8TOARS.
  21. Freitas, M. J.; Korrodi-Gregorio, L.; Esteves, S. L. C.; Fardilha, M.. "TCTEX1D4 interactome: relevance for male reproductive function". Febs Journal 279 (2012): 163-163. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000308128601355&KeyUID=WOS:000308128601355.

Intellectual property

Patent
  1. Fardilha, Margarida; Silva, Joana Vieira; FREITAS, MARIA JOÃO; Howl, John; Jones, Sarah. 2021. "Peptides Modulating Sperm Motility By Modulating The Phosphoprotein Phosphatase 1 Complex.".
    Allowed
  2. Fardilha, M; FREITAS, MARIA JOÃO; Howl, J.; Jones, S; Silva, J.V. 2017. "Modulation of Phosphoprotein Phosphatase 1 complexes in male fertility by protein-protein interaction modulator peptide".
    Allowed
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2018/02/09 Identification and in silico characterization of GSK3 human testis and spermatozoa interactome From protein structure to biological function through interactomics: an integrated view, 2nd edition
(Cantanhede, Portugal)
2017/06/07 GSK3 interactome: a sneak peek into isoform-specific functions of GSK3 in male fertility 3rd Post-Graduate Symposium on Biomedicine
iBiMED (Aveiro, Portugal)

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2021/07/01 - 2021/07/31 Obtain a monoclonal cell line from a population of cells expressing PKD2
Supervisor
Katholieke Universiteit Leuven, Belgium
2021/07/01 - 2021/07/31 Obtain a monoclonal cell line from a population of cells expressing PKD2
Supervisor
Katholieke Universiteit Leuven, Belgium
2020/02/01 - 2020/03/30 Cloning EGFR family members
Supervisor
Katholieke Universiteit Leuven, Belgium
2018/01/02 - 2018/05/31 Cloning of Molecular tools to study PPP1 interactions
Supervisor
Universidade de Aveiro, Portugal

Event organisation

Event name
Type of event (Role)
Institution / Organization
2018/03/22 - 2018/03/24 Member of the Medical Sciences department team on “Open Days 2018”, 22-24th of March, University of Aveiro. (2018/03/22 - 2018/03/24)
Exhibition (Member of the Organising Committee)
Universidade de Aveiro, Portugal
2017/04/27 - 2017/04/27 Organizer of the conference: To move or not to move: How to control sperm motility, 27th April, 2017, University of Aveiro. (2017/04/27 - 2017/04/27)
Conference (Member of the Organising Committee)
Universidade de Aveiro, Portugal