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Identification

Personal identification

Full name
Madalena Maria Vilela Pimentel

Citation names

  • Pimentel, Madalena

Author identifiers

Ciência ID
0F14-3779-9926
ORCID iD
0000-0002-4598-1290

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Upper intermediate (B2) Advanced (C1) Upper intermediate (B2) Upper intermediate (B2)
Education
Degree Classification
2019/12/19
Concluded
 Farmácia (Título de Agregado)
Major in Microbiologia
Universidade de Lisboa Faculdade de Farmácia, Portugal
 Aprovada por Unanimidade
2000/04/17
Concluded
 Farmácia (Doutoramento)
Major in Microbiologia
Universidade de Lisboa Faculdade de Farmácia, Portugal
"Biologia Molecular da Resistência à Superinfecção por Bacteriofagos:Estudo do Gene pin do Micobacteriofago Ms6" (THESIS/DISSERTATION)
 Aprovado com distinção e louvor
1990
Concluded
 Provas Aptidão Pedagógica e Científica (Outros)
Major in Microbiologia
Universidade de Lisboa Faculdade de Farmácia, Portugal
1985
Concluded
 Ciências Farmacêuticas/Análises Químico-Biológicas (Licenciatura)
Universidade de Lisboa Faculdade de Farmácia, Portugal
 15 valores
Affiliation

Science

Category
Host institution 		Employer
2015 - Current Researcher (Research) Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
2000 - 2014 Researcher (Research) Universidade de Lisboa, Faculdade de Farmácia/Associação para o Desenvolvimeto do Ensino e Investigação da Microbiologia/CPM-Unidade dos Retrovirus e Infeções Associadas, Portugal

Teaching in Higher Education

Category
Host institution 		Employer
2023/05 - Current Full Professor (University Teacher) Universidade de Lisboa Faculdade de Farmácia, Portugal
Universidade de Lisboa Faculdade de Farmácia, Portugal
2012 - Current Associate Professor (University Teacher) Universidade de Lisboa Faculdade de Farmácia, Portugal
Universidade de Lisboa Faculdade de Farmácia, Portugal
Projects

Contract

Designation Funders
2018 - Current Clinical significance of Mycobacterium tuberculosis cell wall structural diversity: contribution to novel mechanisms of antibiotic resistance and relevance towards tuberculosis progression and treatment
PTDC/BIA-MIC/31233/2017
Researcher
Faculdade de Farmácia de Lisbia/FarmID, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2023/01/31 - 2024/07/30 AntibacML: Harnessing shared chemical and activity patterns in antimicrobial compounds to build improved computational models for the discovery of new antibiotics
2022.03752.PTDC
10.54499/2022.03752.PTDC
Researcher
Associação da Faculdade de Farmácia para a Investigação e Desenvolvimento, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2019/09/01 - 2022/04/30 FuFighters Fusion of complementary antibacterial functions as a new strategy to generate alternative agents to fight antibiotic-resistant pathogens
PTDC/EMD-EMD/28109/2017
Co-Principal Investigator (Co-PI)
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2019/01/01 - 2019/12/31 Instituto de Investigação do Medicamento
UID/DTP/04138/2019
Instituto Politécnico de Coimbra Centro de Estudos de Recursos Naturais Ambiente e Sociedade, Portugal

Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2013/01/15 - 2015/09/30 Exploring the Mycobacteriophage lysis proteins to destroy the Mycobacteria Cell envelope
PTDC/IMI-MIC/0694/2012
Principal investigator
Universidade de Lisboa, Faculdade de Farmácia/Associação para o Desenvolvimeto do Ensino e Investigação da Microbiologia/CPM-Unidade dos Retrovirus e Infeções Associadas, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2011/01/01 - 2012/12/31 Projecto Estratégico - UI 775 - 2011-2012
PEst-OE/SAU/UI0775/2011
Universitat Autònoma de Barcelona, Spain

Instituto Politécnico de Coimbra Centro de Estudos de Recursos Naturais Ambiente e Sociedade, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2007/07/01 - 2010/12/31 Characterization of the mycobacteriophages lytic genes with antimicrobial activity on mycobacteria
PTDC/SAU-FCF/73017/2006
Principal investigator
Universidade de Lisboa, Faculdade de Farmácia/Associação para o Desenvolvimeto do Ensino e Investigação da Microbiologia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2003/04/15 - 2007/02/28 Functional Analysis of Micobacteriophages Ms6 genes involved in Mycobacteria cell lysis
POCTI/ESP/ 47723/2002
Principal investigator
Universidade de Lisboa, Faculdade de Farmácia/Associação para o Desenvolvimeto do Ensino e Investigação da Microbiologia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Pimentel, Madalena. "Genetics of Phage Lysis". United States, 2014.
    doi:10.1128/9781555818845
Conference abstract
  1. Catalão, Maria João; Rosado, Helena; Pimentel, Madalena; Moniz-Pereira, José. "Changes in the DNA sequence of MS6 bacteriophage attP-int region allows site-specific recombination into the three tRNA Ala genes of M. bovis BCG". Paper presented in 2nd Congress of the Pharmaceutical Sciences_ Revista Portuguesa de Farmácia, Coimbra, 2005.
    Published
  2. Rosado, Helena; Catalão, Maria João; Pimentel, Madalena; Moniz-Pereira, José. "Construction of a new system for DNA expression in Mycobacteria". Paper presented in 2nd Congress of the Pharmaceutical Sciences_ Revista Portuguesa de Farmácia, 2005.
  3. Brízio, A; Da Silva, G.; Duarte, A; Pimentel, Madalena. "Transduction of imipenem resistance by bacteriophages carried by Pseudomonas aeruginosa clinical isolates". Paper presented in 7th European Congress of Chemotherapy and Infection, Florença, 2005.
    Published
  4. Pimentel, Madalena; Rodrigues, Lénia; Moura, Rita; Vital, João; Garcia, Miguel; Moniz-Pereira, José. "Cloning and expression of Mycobacteriophage Ms6 Lytic genes in E.coli.". Paper presented in 1st Congress of the Pharmaceutical Sciences_ Revista Portuguesa de Farmácia, Lisboa, 2003.
    Published
  5. Pimentel, Madalena; Garcia, Miguel,; Moniz-Pereira, José. "Identification of a mycobacteriophage Ms6 DNA gene that confers inhibition to superinfection". Paper presented in 19th Annual Congress of the European Society of Mycobacteriology-Revista Portuguesa de Pneumonologia, Lisboa, 1998.
    Published
  6. Garcia, Miguel; Pimentel, Madalena; Moniz-Pereira, José. "Characterization of a mycobacteriophage Ms6 promoter region". Paper presented in 19th Annual Congress of the European Society of Mycobacteriology-Revista Portuguesa de Pneumonologia, Lisboa, 1998.
    Published
Conference poster
  1. "Identification of a transmembranar protein encoded by the Gene pin of Mycobacteriophage Ms6: Role in Superinfection Exclusion". Paper presented in Workshop Molecular Approaches to Tuberculosis, Madrid, 2020.
  2. Rosa, C; Domingos, A; Asada, M; Pimentel, Madalena; Antunes, S. "Development of Methods for Babesia ovis Genetic Manipulation". Paper presented in International Congress on Tropical Veterinary Medicine and 2nd Joint AITVM-STVM Meeting, 2018.
  3. Gigante, Adriano M; Hampton C; Dillard, RS; Wright ER; Moniz-Pereira, José; Pimentel, Madalena. "Mycobacteriophage Ms6 LysB: an essential lysis protein for an efficient lysis". Paper presented in Molecular Genetics of Bacteria and Phages Meeting, 2016.
  4. Gigante, Adriano M; Hampton C; Dillard, RS; Moniz-Pereira, José; Wright, ER; Pimentel, Madalena. "Mycobacteriophage Ms6: role of LysB on host cell lysis assessed by Cryo-Electron Microscopy". Paper presented in Microbiotec'15, 2015.
  5. Pombo, Sofia; Martins, Francisco; Gigante, Adriano M; Moniz-Pereira, José; Pimentel, Madalena. "Construction of recombinant Mycobacteriophage Ms6 expressing Green Fluorescent Protein using BRED technology". Paper presented in Viruses of Microbes III, 2014.
  6. Gigante, Adriano M; Martins, Francisco; Moniz-Pereira, José; Pimentel, Madalena. "The impact of Ms6 LysB in mycobacteria phage-mediated lysis". Paper presented in Viruses of Microbes III, 2014.
  7. Martins, Francisco; Gigante, Adriano M; Moniz-Pereira, José; Pimentel, Madalena. "Exploring mycobacteriophage-induced lysis: subcellular localization of the Ms6 endolysin LysA and its chaperone". Paper presented in Viruses of Microbes III, 2014.
  8. Gigante, Adriano M; Martins, Francisco; Moniz-Pereira, José; Pimentel, Madalena. "The impact of Ms6 LysB in mycobacteria phage-mediated lysis". Paper presented in Microbiotec'13, 2013.
  9. Martins, Francisco; Gigante, Adriano M; Moniz-Pereira, José; Pimentel, Madalena. "Contribution of Ms6 Gp1 to Mycobacterium smegmatis lysis". Paper presented in Microbiotec'13, 2013.
  10. Pimentel, Madalena; Catalão, Maria João; Gil, Filipa; Martins, Francisco; Pinheiro-Torres, Beatriz; McNeil, M.R.; Moniz-Pereira, José. "Mycobacteriophage Ms6: New insights into the mechanisms of lysis". Paper presented in Viruses of Microbes II, 2012.
  11. Proença, D; Leandro, C.; Silva, F; Santos, S.; Lopes, F; Mato, R; Cavaco-Silva, P.; et al. "Genome sequence analysis and functional study of the endolysins of two bacteriophages with broad lytic spectrum against Enterococcus faecalis clinical strains". Paper presented in Viruses of Microbes II, 2012.
  12. Proença, D; Pimentel, Madalena; Lopes, F; Mata, R; Garcia, Miguel; São-José, C. "Cell wall activity of two endolysins targeting enterococcal clinical strains". Paper presented in The Great Wall Symposium: The Dynamics of Peptidoglycan Strucutre and Function: new Insights into the ‘Great Wall, 2011.
  13. Fernandes, S; Cantante, C.; Pimentel, Madalena; Lencastre, H. "Novel chimerical endolysins with broad antimicrobial activity against MRSA". Paper presented in The Great Wall Symposium: The Dynamics of Peptidoglycan Strucutre and Function: new Insights into the ‘Great Wall, 2011.
  14. Catalão, Maria João; Gil,Filipa; McNeil, M.R.; Moniz-Pereira, José; Pimentel, Madalena. "Bacteriophage Ms6 encodes enzymes that target the cell envelope of mycobacteria". Paper presented in The Great Wall Symposium: The Dynamics of Peptidoglycan Strucutre and Function: new Insights into the ‘Great Wall, 2011.
  15. Fernandes, S; Cantante, C; Pimentel, Madalena; Lencastre, H; São-José, C. "Novel chimerical phage endolysins with antimicrobial activity against MRSA and other pathogenic Gram-positive bacteria". Paper presented in Irish Branch Meeting: Microbial Viruses: Genomics, Evolution and Applications in Ecology, Biotechnology and Medicine, 2011.
  16. Proença, D; Pimentel, Madalena; Lopes, F; Mata, R; Garcia, Miguel; São-José, C. "Phage endolysins with antimicrobial activity against enterococcal clinical isolates". Paper presented in Irish Branch Meeting: Microbial Viruses: Genomics, Evolution and Applications in Ecology, Biotechnology and Medicine, 2011.
  17. Catalão, Maria João; Gil, F; Moniz-Pereira, José; Pimentel, Madalena. "Functional Analysis of the Holin-Like Proteins of Mycobacteriophage Ms6". Paper presented in Irish Branch Meeting: Microbial Viruses: Genomics, Evolution and Applications in Ecology, Biotechnology and Medicine, 2011.
  18. Catalão, Maria João; Milho, C.; Gil, Filipa; Piechota, C.; McNeil, M.R.; Moniz-Pereira, José; Pimentel, Madalena. "The Ms6 phage LysA is an amidase endolysin with activity against Gram positive bacteria". Paper presented in Viruses of Microbes, Institut Pasteur, Paris,, 2010.
  19. Gil, F; Grzegorzewicz, A; Catalão, M.J.; Vital, João; McNeil, M.R.; Pimentel, Madalena. "The mycobacteriophage Ms6 encodes an additional lysis protein (LysB) that targets the outer membrane of Mycobacterium smegmatis". Paper presented in Viruses of Microbes, Institut Pasteur, Paris, France, 2010.
  20. Gil, Filipa; Grzegorzewicz, A; Catalão, Maria João; Vital, João; McNeil, M.R.; Pimentel, Madalena. "Mycobacteriophage Ms6 LysB specifically targets the outer membrane of Mycobacterium smegmatis". Paper presented in Microbiotec'09, 2009.
  21. Catalão, Maria João; Gil, Filipa; Moniz-Pereira, José; Pimentel, Madalena. "The Mycobacteriophage Ms6 Endolysin Reaches Its Target Assisted by Gp1, a Chaperone-Like Protein". Paper presented in 109th General Meeting of the American Society for Microbiology., 2009.
  22. Gil, Filipa; Catalão, Maria João; Vital, João; Moniz-Pereira, José; McNeil, M.R.; Pimentel, Madalena. "LysB: A Lipolytic Enzyme Encoded within the Lysis Module of the Mycobacteriophage Ms6.". Paper presented in 108th General Meeting of the American Society for Microbiology, 2008.
  23. Catalão, Maria João; Gil, Filipa; Moniz-Pereira, José; Pimentel, Madalena. "A chaperone-like protein is involved in the translocation of the mycobacteriophage Ms6 endolysin". Paper presented in Micro-Biotec-XXXIII, 2007.
  24. Gil, Filipa; Catalão, Maria João; Vital, João; Moniz-Pereira, José; McNeil, M.R.; Pimentel, Madalena. "Mycobacteriophage Ms6 LysB: the first lipase described from a bacteriophage". Paper presented in Micro-Biotec-XXXIII JPG 2007, 2007.
  25. "A Phage Lytic Enztme from Mycobacteriophage Ms6 has Antibacterial Activity". Paper presented in 12th International Congress of Infectious Diseases, 2006.
  26. Gil, Filipa; Catalão, Maria João; Vital, João; Moniz-Pereira, José; McNeil, M.R.; Pimentel, Madalena. "Analysis of the lysis genes lysA and lysB of the mycobacteriophage Ms6". Paper presented in 106th General Meeting of the American Society for Microbiology, 2006.
  27. Pimentel, Madalena; Gil, Filipa; Vital, João; Moniz-Pereira, José. "Role of the lytic genes from micobacteriophage Ms6 in cell lysis". Paper presented in Keystone Symposia, Tuberculosis: Integrating Host and Pathogen Biology, 2005.
  28. Pimentel, Madalena; Gil, Filipa; Vital, João; Moniz-Pereira, José. "Overexpression of gene gp1 of the mycobacteriophage Ms6 lytic cassette causes a lethal effect in E. coli". Paper presented in ASM Conference: The New Phage Biology, 2004.
  29. "Genes from the Lytic Operon of Mycobacteriophage Ms6 can induce a lethal effect in E.coli". Paper presented in MICRO´2003, 2003.
  30. Pimentel, Madalena; Rodrigues, Lénia; Vital, João; Garcia, Miguel; Moniz-Pereira, José. "Functional analysis of the lytic system of the mycobacteriophage Ms6 in E.col". Paper presented in 103rd General Meeting of the American Society for Microbiology, 2003.
  31. "The gene pin of Mycobacteriophage Ms6 encodes a membrane protein which is involved in a superinfection resistance mechanism". Paper presented in Segundas Jornadas da Sociedade Portuguesa de Ciências Farmacêuticas, 2001.
  32. Pimentel, Madalena; Anes, Elsa; Miguel Gacia; Moniz-Pereira, José. "Identification of a transmembranar protein encoded by the Gene pin of Mycobacteriophage Ms6: Role in Superinfection Exclusion". Paper presented in Workshop Molecular Approaches to Tuberculosis, Madrid, 2000.
  33. Garcia, Miguel; Pimentel, Madalena; Moniz-Pereira, José. "The Expression of the Mycobacteriophage Ms6 Lytic Functions is Controlled by a Premature Transcription Termination Signal". Paper presented in Tuberculosis 2000, 2000.
  34. Pimentel, Madalena; Garcia, Miguel; Anes, Elsa; Moniz-Pereira, José. "The Protein Pin of Mycobacteriophage Ms6 is Involved in a Resistance to Superinfection Mechanism". Paper presented in Tuberculosis 2000, 2000.
Journal article
  1. Cátia Silveiro; Mariana Marques; Francisco Olivença; David Pires; Diana Mortinho; Alexandra Nunes; Madalena Pimentel; Elsa Anes; Maria João Catalão. "CRISPRi-mediated characterization of novel anti-tuberculosis targets: Mycobacterial peptidoglycan modifications promote beta-lactam resistance and intracellular survival". Frontiers in Cellular and Infection Microbiology 13 (2023): http://dx.doi.org/10.3389/fcimb.2023.1089911.
    10.3389/fcimb.2023.1089911
  2. Francisco Olivença; Cláudia Ferreira; Alexandra Nunes; Cátia Silveiro; Madalena Pimentel; João Paulo Gomes; Maria João Catalão. "Identification of drivers of mycobacterial resistance to peptidoglycan synthesis inhibitors". Frontiers in Microbiology 13 (2022): http://dx.doi.org/10.3389/fmicb.2022.985871.
    10.3389/fmicb.2022.985871
  3. "Chemical Characterization and Bioactivity of Commercial Essential Oils and Hydrolates Obtained from Portuguese Forest Logging and Thinning". Molecules (2022): https://doi.org/10.3390/molecules27113572.
    10.3390/molecules27113572
  4. Adriano M. Gigante; Francisco Olivença; Maria João Catalão; Paula Leandro; José Moniz-Pereira; Sérgio R. Filipe; Madalena Pimentel. "The Mycobacteriophage Ms6 LysB N-Terminus Displays Peptidoglycan Binding Affinity". Viruses 13 7 (2021): 1377-1377. https://doi.org/10.3390/v13071377.
    10.3390/v13071377
  5. Gigante, Adriano M.; Olivença, Francisco; Catalão, Maria João; Leandro, Paula; Moniz-Pereira, José; Filipe, Sérgio R.; Pimentel, Madalena. "The mycobacteriophage ms6 lysb n-terminus displays peptidoglycan binding affinity". (2021): http://hdl.handle.net/10362/131840.
    https://doi.org/10.3390/v13071377
  6. André A. Santos; Marta B. Afonso; Ricardo S. Ramiro; David Pires; Madalena Pimentel; Rui E. Castro; Cecília M.P. Rodrigues. "Host miRNA-21 promotes liver dysfunction by targeting small intestinal Lactobacillus in mice". Gut Microbes 12 1 (2020): 1840766-1840766. https://doi.org/10.1080/19490976.2020.1840766.
    10.1080/19490976.2020.1840766
  7. Tan, Yong Zi; Zhang, Lei; Rodrigues, José; Zheng, Ruixiang Blake; Giacometti, Sabrina I; Rosário, Ana L; Kloss, Brian; et al. "Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria". Molecular Cell (2020):
    Accepted
  8. Rosa, Catarina; Asada, Masahito; Hakimi, Hassan; Domingos, Ana; Pimentel, Madalena; Antunes, Sandra. "Transient transfection of Babesia ovis using heterologous promoters". Ticks and Tick-borne Diseases 10 6 (2019): 101279. http://dx.doi.org/10.1016/j.ttbdis.2019.101279.
    Published • 10.1016/j.ttbdis.2019.101279
  9. Catalão, Maria João; Filipe, Sérgio R.; Pimentel, Madalena; Pimentel, Madalena. "Revisiting anti-tuberculosis therapeutic strategies that target the peptidoglycan structure and synthesis". Frontiers in Microbiology (2019): http://www.scopus.com/inward/record.url?scp=85065905919&partnerID=8YFLogxK.
    https://doi.org/10.3389/fmicb.2019.00190
  10. Pimentel, Madalena. "Mycobacteriophage Lysis Enzymes: Targeting the Mycobacterial Cell Envelope". Viruses (2018): http://www.mdpi.com/1999-4915/10/8/428.
    10.3390/v10080428
  11. Gigante, A.M.; Hampton, C.M.; Dillard, R.S.; Gil, F.; Catalão, M.J.; Moniz-Pereira, J.; Wright, E.R.; Pimentel, M.. "The Ms6 mycolyl-arabinogalactan esterase lysB is essential for an efficient mycobacteriophage-induced lysis". Viruses 9 11 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85034770173&partnerID=MN8TOARS.
    10.3390/v9110343
  12. Pereira, L.; Diogo, J.; Mateus, R.; Pimentel, M.; Videira, M.. "Host-directed strategies using lipid nanoparticles to reduce mycobacteria survival". Journal of Nanoparticle Research 17 2 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84922352823&partnerID=MN8TOARS.
    10.1007/s11051-015-2892-x
  13. Proença, D.; Leandro, C.; Garcia, M.; Pimentel, M.; São-José, C.. "EC300: a phage-based, bacteriolysin-like protein with enhanced antibacterial activity against Enterococcus faecalis". Applied Microbiology and Biotechnology 99 12 (2015): 5137-5149. http://www.scopus.com/inward/record.url?eid=2-s2.0-84929948662&partnerID=MN8TOARS.
    10.1007/s00253-015-6483-7
  14. Mendes, J.J.; Leandro, C.; Mottola, C.; Barbosa, R.; Silva, F.A.; Oliveira, M.; Vilela, C.L.; et al. "In vitro design of a novel lytic bacteriophage cocktail with therapeutic potential against organisms causing diabetic foot infections". Journal of Medical Microbiology 63 PART 8 (2014): 1055-1065. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905174218&partnerID=MN8TOARS.
    10.1099/jmm.0.071753-0
  15. Proença, D.; Velours, C.; Leandro, C.; Garcia, M.; Pimentel, M.; São-Jose´, C.; Proença, Daniela; et al. "A two-component, multimeric endolysin encoded by a single gene". Molecular Microbiology (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84919667310&partnerID=MN8TOARS.
    10.1111/mmi.12857
  16. Pimentel, M.. "Genetics of phage lysis". Microbiology Spectrum 2 1 (2014): 1-13. http://www.scopus.com/inward/record.url?eid=2-s2.0-84958970567&partnerID=MN8TOARS.
    10.1128/microbiolspec. MGM2-0017-2013
  17. Mestre, O.; Hurtado-Ortiz, R.; Dos Vultos, T.; Namouchi, A.; Cimino, M.; Pimentel, M.; Neyrolles, O.; Gicquel, B.. "High Throughput Phenotypic Selection of Mycobacterium tuberculosis Mutants with Impaired Resistance to Reactive Oxygen Species Identifies Genes Important for Intracellular Growth". PLoS ONE 8 1 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84872193109&partnerID=MN8TOARS.
    10.1371/journal.pone.0053486
  18. Mahapatra, S.; Piechota, C.; Gil, F.; Ma, Y.; Huang, H.; Scherman, M.S.; Jones, V.; et al. "Mycobacteriophage Ms6 LysA: A Peptidoglycan Amidase and a Useful Analytical Tool". Applied and Environmental Microbiology 79 3 (2013): 768-773. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873849826&partnerID=MN8TOARS.
    10.1128/AEM.02263-12
  19. Catalão, M.J.; Gil, F.; Moniz-Pereira, J.; São-José, C.; Pimentel, M.. "Diversity in bacterial lysis systems: Bacteriophages Show the way". FEMS Microbiology Reviews 37 4 (2013): 554-571. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878961038&partnerID=MN8TOARS.
    10.1111/1574-6976.12006
  20. Proença, D.; Fernandes, S.; Leandro, C.; Silva, F.A.; Santos, S.; Lopes, F.; Mato, R.; et al. "Phage endolysins with broad antimicrobial activity against Enterococcus faecalis clinical strains". Microbial Drug Resistance 18 3 (2012): 322-332. http://www.scopus.com/inward/record.url?eid=2-s2.0-84862018596&partnerID=MN8TOARS.
    10.1089/mdr.2012.0024
  21. Fernandes, S.; Proença, D.; Cantante, C.; Silva, F.A.; Leandro, C.; Lourenço, S.; Milheiriço, C.; et al. "Novel chimerical endolysins with broad antimicrobial activity against methicillin-resistant Staphylococcus aureus". Microbial Drug Resistance 18 3 (2012): 333-343. http://www.scopus.com/inward/record.url?eid=2-s2.0-84862001592&partnerID=MN8TOARS.
    10.1089/mdr.2012.0025
  22. Catalão, M.J.; Milho, C.; Gil, F.; Moniz-Pereira, J.; Pimentel, M.. "A second endolysin gene is fully embedded in-frame with the lysA gene of mycobacteriophage Ms6". PLoS ONE 6 6 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-79958281245&partnerID=MN8TOARS.
    10.1371/journal.pone.0020515
  23. Catalão, M.J.; Gil, F.; Moniz-Pereira, J.; Pimentel, M.. "The endolysin-binding domain encompasses the N-terminal region of the mycobacteriophage Ms6 Gp1 chaperone". Journal of Bacteriology 193 18 (2011): 5002-5006. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052543330&partnerID=MN8TOARS.
    10.1128/JB.00380-11
  24. Catalão, M.J.; Gil, F.; Moniz-Pereira, J.; Pimentel, M.. "Functional analysis of the Holin-Like proteins of mycobacteriophage Ms6". Journal of Bacteriology 193 11 (2011): 2793-2803. http://www.scopus.com/inward/record.url?eid=2-s2.0-79958121285&partnerID=MN8TOARS.
    10.1128/JB.01519-10
  25. Gil, F.; Grzegorzewicz, A.E.; Catalão, M.J.; Vital, J.; McNeil, M.R.; Pimentel, M.. "Mycobacteriophage Ms6 LysB specifically targets the outer membrane of Mycobacterium smegmatis". Microbiology 156 5 (2010): 1497-1504. http://www.scopus.com/inward/record.url?eid=2-s2.0-77952375559&partnerID=MN8TOARS.
    10.1099/mic.0.032821-0
  26. Catalão, M.J.; Gil, F.; Moniz-Pereira, J.; Pimentel, M.. "The mycobacteriophage Ms6 encodes a chaperone-like protein involved in the endolysin delivery to the peptidoglycan". Molecular Microbiology 77 3 (2010): 672-686. http://www.scopus.com/inward/record.url?eid=2-s2.0-77954850130&partnerID=MN8TOARS.
    10.1111/j.1365-2958.2010.07239.x
  27. Gil, F.; Catalão, M.J.; Moniz-Pereira, J.; Leandro, P.; McNeil, M.; Pimentel, M.. "The lytic cassette of mycobacteriophage Ms6 encodes an enzyme with lipolytic activity". Microbiology 154 5 (2008): 1364-1371. http://www.scopus.com/inward/record.url?eid=2-s2.0-46249097759&partnerID=MN8TOARS.
    10.1099/mic.0.2007/014621-0
  28. Brízio, A.; Conceição, T.; Pimentel, M.; Da Silva, G.; Duarte, A.. "High-level expression of IMP-5 carbapenemase owing to point mutation in the -35 promoter region of class 1 integron among Pseudomonas aeruginosa clinical isolates". International Journal of Antimicrobial Agents 27 1 (2006): 27-31. http://www.scopus.com/inward/record.url?eid=2-s2.0-29944444595&partnerID=MN8TOARS.
    10.1016/j.ijantimicag.2005.08.023
  29. Dos Vultos, T.; Méderlé, I.; Abadie, V.; Pimentel, M.; Moniz-Pereira, J.; Gicquel, B.; Reyrat, J.-M.; Winter, N.. "Modification of the mycobacteriophage Ms6 attP core allows the integration of multiple vectors into different tRNAala T-loops in slow- and fast-growing mycobacteria". BMC Molecular Biology 7 (2006): http://www.scopus.com/inward/record.url?eid=2-s2.0-33846590999&partnerID=MN8TOARS.
    10.1186/1471-2199-7-47
  30. Conceição, T.; Faria, N.; Pimentel, M.; Soveral, G.; Duarte, A.; Lito, L.M.; Melo Cristino, J.; Salgado, M.J.. "New Chromosomal AmpC ß-Lactamase in Enterobacter cloacae [3]". Antimicrobial Agents and Chemotherapy 48 4 (2004): http://www.scopus.com/inward/record.url?eid=2-s2.0-18244426114&partnerID=MN8TOARS.
    10.1128/AAC.48.4.1437.2004
  31. Garcia, M.; Pimentel, M.; Moniz-Pereira, J.. "Expression of mycobacteriophage Ms6 lysis genes is driven by two s70-like promoters and is dependent on a transcription termination signal present in the leader RNA". Journal of Bacteriology 184 11 (2002): 3034-3043. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036098510&partnerID=MN8TOARS.
    10.1128/JB.184.11.3034-3043.2002
  32. Silva, O.; Duarte, A.; Pimentel, M.; Viegas, S.; Barroso, H.; Machado, J.; Pires, I.; Cabrita, J.; Gomes, E.. "Antimicrobial activity of Terminalia macroptera root". Journal of Ethnopharmacology 57 3 (1997): 203-207. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030814426&partnerID=MN8TOARS.
    10.1016/S0378-8741(97)00068-8
  33. Moreira, R.; Calheiros, T.; Cabrita, J.; Mendes, E.; Pimentel, M.; Iley, J.. "Acyloxymethyl as a drug protecting group. Part 3. Tertiary O-amidomethyl esters of penicillin G: Chemical hydrolysis and anti-bacterial activity". Pharmaceutical Research 13 1 (1996): 70-75. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029865020&partnerID=MN8TOARS.
    10.1023/A:1016077200460
  34. Silva, O.; Duarte, A.; Cabrita, J.; Pimentel, M.; Diniz, A.; Gomes, E.. "Antimicrobial activity of Guinea-Bissau traditional remedies". Journal of Ethnopharmacology 50 1 (1996): 55-59. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030030915&partnerID=MN8TOARS.
    10.1016/0378-8741(95)01323-7
  35. Paulo, A.; Pimentel, M.; Viegas, S.; Pires, I.; Duarte, A.; Cabrita, J.; Gomes, E.T.. "Cryptolepis sanguinolenta activity against diarrhoeal bacteria". Journal of Ethnopharmacology 44 2 (1994): 73-77. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028037430&partnerID=MN8TOARS.
    10.1016/0378-8741(94)90071-X
Thesis / Dissertation
  1. Tordo, Tânia Alexandra Carvalho. "Desenvolvimento de embalagens ativas biodegradáveis antioxidantes e antimicrobianas". Master, 2021. http://hdl.handle.net/10451/54750.
  2. Santos, Rafael Luís Pereira. "Analysis of bacteriophage E2 and its enzymes in the control of Escherichia coli biofilms". Master, Universidade de Lisboa Faculdade de Farmácia, 2019. http://hdl.handle.net/10451/42774.
  3. Rosa, Catarina Maria da Silva. "Advances in the development of a stable transfection system for Babesia ovis". Master, 2018. http://hdl.handle.net/10451/39293.
  4. Gigante, Adriano Marcelo Dos Santos. "Mycobacteriophage-mediated lysis : the role of LysB proteins". PhD, 2018. http://hdl.handle.net/10451/35925.
  5. Amorim, Catarina Domingues. "Development of a Phage Cocktail to Prevent Catheter-Associated Urinary Tract Infections". Master, 2017. http://hdl.handle.net/10451/34217.
  6. Miguel, Francisco Olivença. "In silico analysis of mycobacteriophage Ms6". Master, 2017. http://hdl.handle.net/10451/36117.
  7. Augusto, Maria Telma Celerinda. "Terapia fágica : situação atual". Master, 2016. http://hdl.handle.net/10451/34556.
  8. Maksymenko, Vera. "Infeções por Clostridium difficile : causas, sintomas e tratamento". Master, 2016. http://hdl.handle.net/10451/34516.
  9. Pombo, Sofia Pires de Oliveira. "Construction of Ms6 derivative mutants as tools for interaction studies ith mycobacteria". Master, 2015. http://hdl.handle.net/10451/17840.
  10. Martins, Francisco André de Lemos. "Mycobacteriophage Ms6 : exploring the involvement of Gp1 on LysA export". Master, 2014. http://hdl.handle.net/10451/15916.
  11. Matos, Sandra Cristina Machado de. "Distribuição de exotoxinas em estreptococos dos grupos C e G de Lancefield : comparação entre estirpes responsáveis por infeções no Homem e em animais". Master, 2013. http://hdl.handle.net/10451/11352.
  12. Torres, Maria Beatriz Palacios Rumsey Pinheiro. "Aplicações dos bacteriófagos: avanços recentes e questões regulamentares". Master, 2013. http://hdl.handle.net/10451/46003.
  13. Silva, Alessa Ede Valverde da, 1987-. "Detection and localization of LysA and GP1 proteins of the mycobacteriophage Ms6". Master, 2011. http://hdl.handle.net/10451/6137.
  14. Martins, Vitor Manuel Antunes. "Avaliação de risco do bacalhau demolhado congelado comercializado em Portugal". Master, 2007. http://hdl.handle.net/10451/12397.
  15. Pimentel, Madalena. "Biologia Molecular da Resistência à Superinfeção por Bacteriófagos: Estudo do Gene pin do Micobacteriófago Ms6". PhD, Universidade de Lisboa Faculdade de Farmácia, 2000.

Intellectual property

Patent
  1. Garcia, Miguel; Pimentel, Madalena; São José, C. 2011. "Enterococcal Phage Peptides And Methods of Use Thereof".
  2. Pimentel, Madalena; Garcia, Miguel; São-José, C. 2010. "Antibacterial Phage Peptides And Methods of Use Thereof". Portugal.
    Withdrawn
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2021/11/23 Mycobacteriophage Ms6 LysB: a lipolytic lysis enzyme with Peptidoglycan Binding Affinity MicroBiotec21
Sociedade Portuguesa de Microbiologia (Lisboa, Portugal)
2020/01/31 How mycobacteriophage Ms6 eliminates the mycobacteria envelope barriers II Simpósio de Investigação em Tuberculose e Micobactérias não Tuberculosas
Universidade de Lisboa Faculdade de Farmácia (Lisboa, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2023 - 2023 Opportunities and Regulatory Challenges of Phage Therapy
Supervisor
 Regulação e Avaliação do Medicamento e Produtos de Saúde (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2023 - 2023 Hydrogels as vehicles for the topical application of therapeutic bacteriophages
Co-supervisor
 Ciências Farmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2022 - 2023 Listeria monocytogenes, Escherichia coli e Estafilococos coagulase positiva em queijos alentejanos curados de leite cru
Co-supervisor
 Mestrado em Qualidade ALimentar e Saúde (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2022 - 2023 Otimização da germinação de leguminosas com vista à melhoria da composição nutricional e da segurança microbiológica
Co-supervisor
 Mestrado em Qualidade Alimentar e Saúde (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2022 - 2022 Bacteriófagos e a microbiota intestinal
Supervisor
 Ciências Farmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2022 - 2022 O Potencial Terapêutico dos Bacteriófagos e as Infeções Nosocomiais
Supervisor
 Ciências Farmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2022 - 2022 Klebsiella pneumoniae e a ameaça da resistência aos antibióticos
Supervisor
 Ciências Farmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2022 - 2022 Mycobacteriophage Ms6 holin like proteins: investigating the role in endolysin function
Supervisor
 Ciências Biofarmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2019 - 2019 Analysis of bacteriophage E2 and its enzymes in the control of Escherichia coli biofilms
Supervisor
 Ciências Biofarmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2017 - 2017 Development of a Phage Cocktail to Prevent Catheter-Associated Urinary Tract Infections
Supervisor
 Ciências Biofarmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2015 - 2015 Construction of Ms6 derivative mutants as tools for interaction studies in mycobacteria
Supervisor
 Ciências Biofarmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2014 - 2014 Mycobacteriophage-medated lysis: the role of LysB proteins
Supervisor
 Farmácia (PhD)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2014 - 2014 Mycobacteriophage Ms6: Exploring the involvement of Gp1 on LysA export
Supervisor
 Ciências Biofarmacêuticas (Master)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2012 - 2012 Mycobacterium tuberculosis host adaptation and evolution reflected by defense mechanisms against oxidative stress
Co-supervisor
 Farmácia (PhD)
Universidade de Lisboa, Portugal
2010 - 2010 Functional Analysis of LysB Gene from the lysis module of Mycobacteriophage Ms6
Supervisor
 Farmácia (PhD)
Universidade de Lisboa, Portugal
2005 - 2005 Characterization and Functional Analysis of the Accessory Lysis Protein Gp1 in Mycobacterium Smegmatis Infection by Mycobacteriophages Ms6
Supervisor
 Farmácia (PhD)
Universidade de Lisboa, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2021/12 - 2022/07 iVoM season2: In collaboration, the International Society for Viruses of Microorganisms (ISVM), Phage Directory, and the organizers of VoM 2022 (Madalena Pimentel and Joana Azeredo) announce Season 2 of iVoM, a series of online lectures from prominent researchers studying viruses of microbes. Seven sessions will run from December 2021 to May 2022, in the lead-up to the exciting in-person version of VoM, July 2022. (2021/12 - 2022/07)
Conference (Co-organisor)
 Universidade de Lisboa Faculdade de Farmácia, Portugal

Universidade do Minho Centro de Engenharia Biológica, Portugal
2020/06/01 - 2021/03/04 iVoM20 is a series of 6 webminars under the title "Viruses of Microbes 2020 Webinar Series : Take a walk at the VoM side" organized by Madalena Pimentel from the University of Lisbon and Joana Azeredo from the University of Minho with the support of ISVM and Phage Directory. The six web events spotlighting the most recent advances in ecology and evolution of microbial viruses, virus structures and function, virus-host interaction, agro-food, veterinary and environmental biotechnology applications, covered the 6 VoM2020 topics and presented monthly. (2020/09/17 - 2021/03/04)
Conference (Co-organisor)
 Universidade de Lisboa Faculdade de Farmácia, Portugal

Universidade do Minho Centro de Engenharia Biológica, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2022/01/20 Evaluation of cutting-edge diagnostic and treatment monitoring tools in a cohort of tuberculosis patients during the first month of treatment
(Thesis) Main arguer
 Edson Tereso Vany Francisco Mambuque (Master)
Universidade Nova de Lisboa Instituto de Higiene e Medicina Tropical, Portugal
2019/02/14 Using synthetic biology tools to create a compact safe phage for Salmonella control in foods
(Thesis) Main arguer
 Ana Catarina Rodrigues Milho (PhD)
Universidade do Minho Departamento de Engenharia Biológica, Portugal
2016 Using synthetic biology tools to create a compact safe phage for Salmonella control in foods
(Thesis) Main arguer
 Ana Catarina Rodrigues Milho (PhD)
Universidade do Minho, Portugal
2013 Proof of concept of PhageDuction: heterologous transduction mediated by bacteriophage SPP1
(Thesis) Main arguer
 Nuno Filipe Brito Pais dos Santos (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2012 Bacteriophage lytic enzymes and their engineering towards improved antibacterial efficacy
Thesis Member
 Daniela Sofia Moreira Proença (PhD)
Universidade de Lisboa Faculdade de Farmácia, Portugal
2012 Molecular studies on bacteriophage endolysins and their potential to control Gram-negative bacteria
(Thesis) Main arguer
 Hugo Alexandre Mendes de Oliveira (PhD)
Universidade do Minho, Portugal

Journal scientific committee

Journal title (ISSN) Publisher
2023 - 2024 Scientific Advances
2022 - 2022 Microbiological Research
2022 - 2022 Nature Chemical Biology
2021 - 2021 Biochimica e Biophysica Acta: Proteins and Proteomics
2021 - 2021 Microbiology