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Filipe Carvalho has a BSc degree in Biochemistry from the University of Porto (Porto, Portugal). He then joined the lab of Didier Cabanes, at the IBMC, later i3S (Porto, Portugal), to begin his research on the virulence mechanisms and determinants of the human bacterial pathogen Listeria monocytogenes, as an undergraduate and then as a doctoral student. In 2016, he obtained his PhD degree in Biomedical Sciences (University Porto, Portugal), with a thesis on the role of wall teichoic acid glycosylations in the virulence and host immune evasion properties of L. monocytogenes. Between 2016 and 2019, he did a postdoc at the Institut Pasteur (Paris, France), in the lab of Pascale Cossart, studying the impact of L. monocytogenes infection in mitochondrial function and dynamics. He is currently a postdoc at the INRAE (Jouy-en-Josas, France), in the lab of Hélène Bierne, studying the viable-but-non-culturable (VBNC) state of L. monocytogenes. Filipe Carvalho has produced and co-authored several publications in high-impact-factor, international peer-reviewed journals (e.g. PLoS Pathogens, mBio, Frontiers in Cellular and Infection Microbiology, P.N.A.S., Nucleic Acids Research, Environmental Microbiology) and was a reviewer for multiple manuscripts submitted for publication (e.g. Cell Host & Microbe, Toxins, Pathogens). Having established his position as a researcher in the Listeria field, Filipe Carvalho's research interests include further exploring the role of yet-unknown and uncharacterised bacterial factors and processes in the physiology and virulence of Gram-positive bacterial pathogens, using L. monocytogenes as a model organism.
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Identification

Personal identification

Full name
Filipe Freitas Coelho Henriques de Carvalho

Citation names

  • Carvalho F
  • Carvalho, Filipe

Author identifiers

Ciência ID
111E-F09E-33C4
ORCID iD
0000-0002-6828-1772

Email addresses

  • filipe.carvalho@inrae.fr (Professional)

Addresses

  • 4 avenue Jean Jaurès, 78350, Jouy-en-Josas, Yvelines, France (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Microbiology
  • Natural sciences - Biological Sciences - Cell Biology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Proficiency (C2) Proficiency (C2) Proficiency (C2) Proficiency (C2)
French Intermediate (B1) Upper intermediate (B2) Upper intermediate (B2) Intermediate (B1)
Education
Degree Classification
2010 - 2016
Concluded
 Ciências Biomédicas (Doutoramento)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
"Role of wall teichoic acid L-rhamnosylation in Listeria monocytogenes resistance to antimicrobial peptides and surface protein anchoring " (THESIS/DISSERTATION)
 Approved with distinction
2002 - 2006
Concluded
 Bioquímica (Licenciatura)
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
"Study of the interaction of transthyretin with novel protein ligands" (THESIS/DISSERTATION)
 15/20
Affiliation

Science

Category
Host institution 		Employer
2020/08/01 - Current Postdoc (Research) Microbiologie de l'Alimentation au Service de la Santé, France
Microbiologie de l'Alimentation au Service de la Santé, France
2016/11 - 2019/11 Postdoc (Research) Institut Pasteur, France
Institut Pasteur, France
2013/12 - 2014/07 Research Trainee (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2009/11 - 2013/10 Research Trainee (Research) Fundação para a Ciência e a Tecnologia, Portugal
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2007/09 - 2009/10 Research Trainee (Research) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
Projects

Grant

Designation Funders
2011/04/01 - 2014/09/30 Unraveling the interplay between Listeria monocytogenes infection and the host cell cycle
111215
PhD Student Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2011/03/01 - 2014/08/31 LISTRESS. Analysis of the cellular mechanisms underlying the early response of the host to stress induced by Listeria infection: Teichoic acid modifications, surface protein anchoring and virulence.
116472
PhD Student Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2011/08/15 - 2014/08/14 Characterization of two new Listeria monocytogenes virulence genes up-regulated during in vivo infection: role of cadmium efflux system and teichoic acid decoration in Listeria pathogenicity
111581
PhD Student Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2007/09/21 - 2011/01/31 Identification of novel bacterial factors implicated in the virulence of Listeria monocytogenes
65406
Research Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2020/08/01 - 2021/12/31 Molecular determinants and markers of the Listeria monocytogenes viable but non-culturable state
N/A
Post-doc
2016/11/21 - 2019/11/20 Study of the mechanisms of Listeria monocytogenes infection using mass spectrometry
ANR-10-LABX-62-IBEID
Post-doc Fellow
Institut Pasteur, France
 Agence nationale de la recherche
Concluded
Outputs

Publications

Book chapter
  1. Carvalho, Filipe; Pucciarelli, María Graciela; Portillo, Francisco García-del; Cabanes, Didier; Cossart, Pascale. "Extraction of Cell Wall-Bound Teichoic Acids and Surface Proteins from Listeria monocytogenes". In Methods in Molecular Biology, 289-308. Humana Press, 2012.
    Published • 10.1007/978-1-62703-245-2_18
Conference poster
  1. Carvalho, Filipe; Stavru, Fabrizia; Cossart, Pascale. "Search for novel host factors implicated in Drp1-independent mitochondrial fission induced by Listeria monocytogenes". Paper presented in 15th Annual EIMID Meeting, 2018.
  2. Carvalho, Filipe; Stavru, Fabrizia; Cossart, Pascale. "Searching for novel host factors implicated in Listeria monocytogenes-induced Drp1-independent mitochondrial fission". Paper presented in 14th Annual EIMID Meeting, 2017.
  3. Carvalho, Filipe; Atilano, Magda L.; Camejo, Ana; Gallo, Richard L.; Filipe, Sérgio R.; Sousa, Sandra R.; Cabanes, Didier. "L-rhamnosylation of Listeria monocytogenes wall teichoic acids promotes resistance to antimicrobial peptides by delaying interaction with the bacterial membrane". Paper presented in 4th I3S Annual Meeting, 2014.
  4. Carvalho, Filipe; Atilano, Magda L.; Camejo, Ana; Gallo, Richard L.; Filipe, Sérgio R.; Sousa, Sandra R.; Cabanes, Didier. "L-rhamnosylation of Listeria monocytogenes wall teichoic acids promotes resistance to cationic antimicrobial peptides by delaying their interaction with the bacterial membrane". Paper presented in 5th EMBO Meeting, 2013.
  5. Carvalho, Filipe; Camejo, Ana; Atilano, Magda L.; García-del Portillo, Francisco; Pucciarelli, María Graciela; Filipe, Sérgio R.; Sousa, Sandra R.; Cabanes, Didier. "L-rhamnosylation of Listeria monocytogenes wall teichoic acids promotes resistance to cationic antimicrobial peptides by delaying their interaction with the bacterial membrane". Paper presented in ISOPOL XVIII, 2013.
  6. Carvalho, Filipe; Camejo, Ana; Atilano, Magda L.; García-del Portillo, Francisco; Pucciarelli, María Graciela; Filipe, Sérgio R.; Sousa, Sandra R.; Cabanes, Didier. "Wall teichoic acid rhamnosylation and Listeria monocytogenes virulence: role in resistance to host defense peptides and surface protein anchoring". Paper presented in 3rd I3S Scientific Retreat, 2012.
  7. Carvalho, Filipe; Camejo, Ana; Ferreira, Pierre; Atilano, Magda L.; Filipe, Sérgio R.; Sousa, Sandra R.; Cabanes, Didier. "Listeria monocytogenes wall teichoic acid rhamnosylation is required for resistance to host defense and virulence". Paper presented in 2nd I3S Scientific Retreat, 2011.
  8. Carvalho, Filipe; Camejo, Ana; Ferreira, Pierre; Cabanes, Didier. "Identification of LvfH, a novel Listeria monocytogenes virulence factor highly activated during infection". Paper presented in 2nd IBMC Scientific Retreat, 2009.
Journal article
  1. Spier, Anna; Connor, Michael G; Steiner, Thomas; Carvalho, Filipe; Cossart, Pascale; Eisenreich, Wolfgang; Wai, Timothy; Stavru, Fabrizia. "Mitochondrial respiration restricts Listeria monocytogenes infection by slowing down host cell receptor recycling". Cell Reports (2021):
    Under revision • 10.1016/j.celrep.2021.109989
  2. Diana Meireles; Rita Pombinho; Filipe Carvalho; Sandra Sousa; Didier Cabanes; Meireles, Diana; Pombinho, Rita; et al. "Listeria monocytogenes Wall Teichoic Acid Glycosylation Promotes Surface Anchoring of Virulence Factors, Resistance to Antimicrobial Peptides, and Decreased Susceptibility to Antibiotics". Pathogens 9 4 (2020): 290. https://www.mdpi.com/2076-0817/9/4/290.
    Open access • Published • 10.3390/pathogens9040290
  3. Carvalho, Filipe; Spier, Anna; Chaze, Thibault; Matondo, Mariette; Cossart, Pascale; Stavru, Fabrizia; Carvalho, Filipe. "Listeria monocytogenes Exploits Mitochondrial Contact Site and Cristae Organizing System Complex Subunit Mic10 To Promote Mitochondrial Fragmentation and Cellular Infection". mBio 11 1 (2020): https://publons.com/publon/32638798/.
    Published • 10.1128/MBIO.03171-19
  4. Costa, Ana Catarina; Carvalho, Filipe; Cabanes, Didier; Sousa, Sandra. "Stathmin recruits tubulin to Listeria monocytogenes-induced actin comets and promotes bacterial dissemination". Cellular and Molecular Life Sciences 76 5 (2018): 961-975. http://dx.doi.org/10.1007/s00018-018-2977-7.
    Published • 10.1007/s00018-018-2977-7
  5. Carvalho, Filipe; Sousa, Sandra; Cabanes, Didier. "L-Rhamnosylation of wall teichoic acids promotes efficient surface association of Listeria monocytogenes virulence factors InlB and Ami through interaction with GW domains". Environmental Microbiology 20 11 (2018): 3941-3951. http://dx.doi.org/10.1111/1462-2920.14351.
    Published • 10.1111/1462-2920.14351
  6. Malet, Julien Karim; Impens, Francis; Carvalho, Filipe; Hamon, Mélanie Anne; Cossart, Pascale; Ribet, David. "Rapid Remodeling of the Host Epithelial Cell Proteome by the Listeriolysin O (LLO) Pore-forming Toxin". Molecular & Cellular Proteomics 17 8 (2018): 1627-1636. http://dx.doi.org/10.1074/mcp.ra118.000767.
    Open access • Published • 10.1074/mcp.ra118.000767
  7. Pinheiro, Jorge; Lisboa, Johnny; Pombinho, Rita; Carvalho, Filipe; Carreaux, Alexis; Brito, Claudia; Pontinen, Anna; et al. "MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence". Nucleic Acids Research (2018): https://publons.com/publon/19558187/.
    10.1093/NAR/GKY624
  8. Duval, Melodie; Dar, Daniel; Carvalho, Filipe; Rocha, Eduardo P. C.; Sorek, Rotem; Cossart, Pascale; Duval, Mélodie. "HflXr, a homolog of a ribosome-splitting factor, mediates antibiotic resistance". Proceedings of the National Academy of Sciences 115 52 (2018): 13359-13364. https://publons.com/publon/25683741/.
    Published • 10.1073/PNAS.1810555115
  9. Pombinho, Rita; Camejo, Ana; Vieira, Ana; Reis, Olga; Carvalho, Filipe; Almeida, Maria Teresa; Pinheiro, Jorge Campos; Sousa, Sandra; Cabanes, Didier. "Listeria monocytogenes CadC Regulates Cadmium Efflux and Fine-tunes Lipoprotein Localization to Escape the Host Immune Response and Promote Infection". The Journal of Infectious Diseases 215 9 (2017): 1468-1479. http://dx.doi.org/10.1093/infdis/jix118.
    Open access • Published • 10.1093/infdis/jix118
  10. Pinheiro, Jorge; Reis, Olga; Vieira, Ana; Moura, Ines M.; Zanolli Moreno, Luisa; Carvalho, Filipe; Pucciarelli, M. Graciela; et al. "Listeria monocytogenes encodes a functional ESX-1 secretion system whose expression is detrimental to in vivo infection". Virulence 8 6 (2016): 993-1004. http://dx.doi.org/10.1080/21505594.2016.1244589.
    Open access • Published • 10.1080/21505594.2016.1244589
  11. Carvalho, Filipe; Atilano, Magda L.; Pombinho, Rita; Covas, Gonçalo; Gallo, Richard L.; Filipe, Sérgio R.; Sousa, Sandra; Cabanes, Didier. "L-Rhamnosylation of Listeria monocytogenes Wall Teichoic Acids Promotes Resistance to Antimicrobial Peptides by Delaying Interaction with the Membrane". PLOS Pathogens 11 5 (2015): e1004919. http://dx.doi.org/10.1371/journal.ppat.1004919.
    Open access • Published • 10.1371/journal.ppat.1004919
  12. Carvalho, Filipe; Sousa, Sandra; Cabanes, Didier. "How Listeria monocytogenes organizes its surface for virulence". Frontiers in Cellular and Infection Microbiology 4 (2014): http://dx.doi.org/10.3389/fcimb.2014.00048.
    Open access • Published • 10.3389/fcimb.2014.00048
  13. Quereda, Juan J.; Pucciarelli, M. Graciela; Botello-Morte, Laura; Calvo, Enrique; Carvalho, Filipe; Bouchier, Christiane; Vieira, Ana; et al. "Occurrence of mutations impairing sigma factor B (SigB) function upon inactivation of Listeria monocytogenes genes encoding surface proteins". Microbiology 159 Pt_7 (2013): 1328-1339. http://dx.doi.org/10.1099/mic.0.067744-0.
    Open access • Published • 10.1099/mic.0.067744-0
  14. Machado, Henrique; Lourenço, António; Carvalho, Filipe; Cabanes, Didier; Kallipolitis, Birgitte H.; Brito, Luísa. "The Tat Pathway Is Prevalent in Listeria monocytogenes Lineage II and Is Not Required for Infection and Spread in Host Cells". Journal of Molecular Microbiology and Biotechnology 23 3 (2013): 209-218. http://dx.doi.org/10.1159/000348245.
    Published • 10.1159/000348245
  15. Henriques, Ana; Carvalho, Filipe; Pombinho, Rita; Reis, Olga; Sousa, Sandra; Cabanes, Didier. "PCR-based screening of targeted mutants for the fast and simultaneous identification of bacterial virulence factors". BioTechniques 53 1 (2012): 1-7. http://dx.doi.org/10.2144/000113906.
    Open access • Published • 10.2144/000113906
  16. Camejo, Ana; Carvalho, Filipe; Reis, Olga; Leitão, Elsa; Sousa, Sandra; Cabanes, Didier. "The arsenal of virulence factors deployed byListeria monocytogenesto promote its cell infection cycle". Virulence 2 5 (2011): 379-394. http://dx.doi.org/10.4161/viru.2.5.17703.
    Open access • Published • 10.4161/viru.2.5.17703
  17. Camejo, Ana; Buchrieser, Carmen; Couvé, Elisabeth; Carvalho, Filipe; Reis, Olga; Ferreira, Pierre; Sousa, Sandra; Cossart, Pascale; Cabanes, Didier. "In Vivo Transcriptional Profiling of Listeria monocytogenes and Mutagenesis Identify New Virulence Factors Involved in Infection". PLoS Pathogens 5 5 (2009): e1000449. http://dx.doi.org/10.1371/journal.ppat.1000449.
    Open access • Published • 10.1371/journal.ppat.1000449

Other

Other output
  1. Listeria monocytogenes exploits the MICOS complex subunit Mic10 to promote mitochondrial fragmentation and cellular infection. 2019. Filipe Carvalho; Anna Spier; Thibault Chaze; Mariette Matondo; Pascale Cossart; Fabrizia Stavru. https://doi.org/10.1101/712067.
    10.1101/712067
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2011/06/27 Listeria monocytogenes wall teichoic acid rhamnosylation is required for resistance to host defense and virulence. 4th Congress of European Microbiologists – FEMS 2011
(Genebra, Switzerland)
2010/05/08 The lvfH gene of Listeria monocytogenes encodes a novel virulence factor highly activated during infection. XVII ISOPOL - International Symposium on Problems of Listeriosis
(Porto, Portugal)
2009/11/28 The lvfH gene of Listeria monocytogenes encodes a novel virulence factor highly activated during infection MicroBiotec'09
Sociedade Portuguesa de Microbiologia; Sociedade Portuguesa de Biotecnologia (Vilamoura, Portugal)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2011 - 2011 Identification and characterization of new Listeria monocytogenes virulence factors
Co-supervisor
 Master Infectiologie : microbiologie, virologie, immunologie (Master)
Université de Paris UFR Sciences du Vivant, France

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2018/07/23 - 2018/07/27 This course is designed for all the students, researchers and academics who would like to get a deeper understanding on synthesis and functionalization of nanoparticles and their use in proteomics. Basic Bioinformatics in proteomics and quantitative methodologies in proteomics are also learnt.
Workshop
 NanoProteomics Summer Course 2018
Universidade Nova de Lisboa Departamento de Física, Portugal
2017/07/03 - 2017/07/07 The MaxQuant Summer School provides hands-on training in computational analysis of proteomics data generated by high-resolution mass spectrometry. The practical part enables participants to perform sophisticated quantitative data analysis in their own future research using the publicly available MaxQuant software (Cox, J. and Mann, M. (2008).
Workshop
 MaxQuant Summer School 2017
Max-Planck-Institut für Biochemie, Germany
2016/12/04 - 2016/12/09 This course, run jointly with Wellcome Genome Campus Advanced Courses and Scientific Conferences, provides hands-on training in the basics of mass spectrometry and proteomics bioinformatics, search engines and post-processing software, quantitative approaches, MS data repositories, the use of public databases for protein analysis, annotation of subsequent protein lists and incorporation of information from molecular interaction and pathway databases.
Workshop
 Proteomics Bioinformatics
European Bioinformatics Institute, United Kingdom
2008/05 - 2008/05 This course follows the recommendations of FELASA for laboratory animal science training for scientists (Category C, now Functions A, B and D). The course combines theory (lectures, self-study) with practical exercises and group assignments. Focus is on how to design, plan and carry out experiments to minimize animal harm. We organize this 80-h course either over 2 subsequent weeks or as modules of 3-5 days over one month. This course will give you the necessary training to obtain a permit to work with animals in most European countries.
Workshop
 Course in Laboratory Animal Handling – Category C accreditation (FELASA)
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Other jury / evaluation

Activity description Institution / Organization
2018 - Current Scientific refereeing for peer-reviewed journals (Cell Host & Microbe, Frontiers in Cellular Microbiology and Infection, Toxins, Pathogens, Microorganisms, Research in Microbiology)
Distinctions

Award

2013 Best Poster Award - XVIII International Symposium on Problems of Listeriosis (ISOPOL)

Other distinction

2013 EMBO Travel Grant - 5th EMBO Meeting (Amsterdam, 21-24 September 2013)
European Molecular Biology Organization, Germany