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Identification

Personal identification

Full name
Margarida Sofia Pereira Duarte Amaral

Citation names

  • Amaral, Margarida
  • Margarida D. Amaral

Author identifiers

Ciência ID
1518-1311-A7C1
ORCID iD
0000-0002-0828-8630

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
French Advanced (C1) Advanced (C1) Intermediate (B1) Advanced (C1)
Spanish; Castilian Advanced (C1) Intermediate (B1) Intermediate (B1) Advanced (C1)
German Beginner (A1) Beginner (A1) Beginner (A1) Beginner (A1)
Education
Degree Classification
2006 - 2006
Concluded
 Bioquímica (Título de Agregado)
Major in Biologia de Sistemas
Universidade de Lisboa Faculdade de Ciências, Portugal
 Unanimously Approved
1993
Concluded
 Bioquímica (Doutoramento)
Major in Sem especialidade
Universidade de Lisboa, Portugal
"Proteínas de Stress: Indução e Regulação em Tetrahymena Pyriformis" (THESIS/DISSERTATION)
 Suma cum Laude
1984 - 1986
Concluded
 Bioquímica (Mestrado)
Major in Genética Molecular
Instituto Gulbenkian de Ciência, Portugal
"The Response of the Protozoan Tetrahymena pyriformis to a Stress Agent: Sodium Meta-Arsenite" (THESIS/DISSERTATION)
 Muito Bom
1976 - 1983
Concluded
 Química (Licenciatura)
Major in Bioquímica
Universidade de Lisboa Faculdade de Ciências, Portugal
"Lipid Content of Liver and Blood Plasma of Hepatomized Rats" (THESIS/DISSERTATION)
 17
Affiliation

Teaching in Higher Education

Category
Host institution 		Employer
2013 - 2028/08/06 Full Professor (University Teacher) Universidade de Lisboa Faculdade de Ciências, Portugal

Positions / Appointments

Category
Host institution 		Employer
2018 - 2022/03/04 Conselho científico/técnico-científico ou orgão correspondente Universidade de Lisboa, Portugal
2015 - 2022 Coordenação ou direção de centro de investigação, departamento ou equivalente Universidade de Lisboa Faculdade de Ciências, Portugal
2016 - 2018 Conselho científico/técnico-científico ou orgão correspondente Universidade de Lisboa, Portugal
2013 - 2016 Conselho científico/técnico-científico ou orgão correspondente Universidade de Lisboa Faculdade de Ciências, Portugal
2013 - 2014 Coordenação ou direção de centro de investigação, departamento ou equivalente Universidade de Lisboa Faculdade de Ciências, Portugal
Projects

Grant

Designation Funders
2014/01 - Current Anoctamin 6 mediates Effects downstream of P2X7-Receptors, essential for Macrophage function and Innate Immunity
grant.256953317
 Deutsche Forschungsgemeinschaft
2015/09 - 2018/08 Novel Factors of CFTR Traffic Related to Epithelial Cell Differentiation: Potential Therapeutic Targets for Cystic Fibrosis
grant.Provided by PTCRIS: 140757
 Stichting Weten
2014/04 - 2015/07 Dissecting the endoplasmic reticulum quality control - differential protein interactions as new therapeutic targets in Cystic Fibrosis
EXPL/BIM-MEC/1451/2013
Researcher
Fundação para a Ciência e a Tecnologia
2014/04 - 2015/07 Dissecting the endoplasmic reticulum quality control - differential protein interactions as new therapeutic targets in Cystic Fibrosis
grant.Provided by PTCRIS: 134476
 Stichting Weten

Contract

Designation Funders
2013 - Current Incidence of CFTR mutations in Individuals with Chronic Respiratory Diseases and their Effect in Disease Expression
MED-2012-022
Principal investigator
Gilead Sciences Lda
Concluded
2017 - 2023 HIT-CF - Personalised Treatment For Cystic Fibrosis Patients With Ultra-rare CFTR Mutations
H2020-SC1-2017-755021
Researcher
European Union
Ongoing
2018 - 2022 Personalised Therapies for all: Restoring airway function in CF using Alternative Chloride Channels
SRC 013
Researcher
Cystic Fibrosis Trust
Ongoing
2020/01/01 - 2021/12/31 PTSense: - Novel Compounds as Potential Drugs for CFTR PTC Mutations
AMARAL19G0
Principal investigator
Cystic Fibrosis Foundation
Ongoing
2019 - 2021 Identification of novel F508del-CFTR traffic correctors among FDA-approved drugs
No ref
Other
Gilead Sciences Inc
Ongoing
2018 - 2021 iDrugCF - Identification of New Drugs for Cystic Fibrosis
PTDC/MED-QUI/2017/28800
Principal investigator
Fundação para a Ciência e a Tecnologia
Ongoing
2020/01/01 - 2020/12/31 Identification of Portuguese patients with Cystic Fibrosis by Complete CFTR Gene Mutation Genotyping and Rectal Biopsy Analyse
No ref
Principal investigator
Vertex Pharmaceuticals SI
Ongoing
2019/01 - 2019/12 BioISI-Biosystems & Integrative Sciences Institute
UID/Multi/04046/2019
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2016 - 2019 DiffTarget-Novel Factors of CFTR Traffic Related to Epithelial Cell Differentiation: Potential Therapeutic Targets for Cystic Fibrosis
PTDC/BIM-MEC/2131/2014
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2016 - 2019 MIMED - Mining the Molecular Metric Space for Drug Design
PTDC/EEI-ESS/4923/2014
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2016 - 2019 FARMTRANSANION-Anion transmembrane transport promoted by drug-like molecules: building a library of anion carriers inspired in Ataluren (PTC124)
PTDC/QEQ-SUP/4283/2014
Researcher
Universidade de Aveiro, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2015 - 2019 INSTINCT - Induced Pluripotent Stem Cells for Identification of Novel Drug Combinations Targeting Cystic Fibrosis Lung and Liver Disease
ERARE15-pp-010/JTC 2015
 Fundação para a Ciência e a Tecnologia
2014 - 2019 INOVCF- Innovative non-CFTR Approaches for Cystic Fibrosis Therapies
SRC 003
Researcher
Cystic Fibrosis Trust
Concluded
2015/01 - 2018/12 BioISI - Biosystems & Integrative Sciences Institute
grant.Provided by PTCRIS: 147256
UID/Multi/04046/2013
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2016 - 2018 Characterization of Orphan CFTR mutations
AMARAL16I0
Principal investigator
Cystic Fibrosis Foundation
Concluded
2016 - 2018 RNA LIFE - Novel RNA Regulators as Potential Drug Targets for Cystic Fibrosis
AMARAL15XX1
Principal investigator
Cystic Fibrosis Foundation
Concluded
2015 - 2017 CFTR mRNA Stability Studies for PTC Mutations
AMARAL15XX0
Principal investigator
Cystic Fibrosis Foundation
Concluded
2016/01/01 - 2016/12/31 Complete CFTR gene mutation analysis in Portuguese patients with Cystic Fibrosis
no ref
Principal investigator
Vertex Pharmaceuticals SI
Concluded
2016/01/01 - 2016/12/31 Predicting Clinical Drug Efficacy of CFTR Protein Modulators Using Intestinal Organoids and Nasal Cells from Patients with Cystic Fibrosis
PGG/008/2015
Principal investigator
Gilead Sciences Lda
Concluded
2012/01/01 - 2015/12/31 Diagnosis, Prognosis and Treatment of Cystic Fibrosis
CAPES- 053/2012
Principal investigator
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Concluded
2014/01 - 2015/12 Incentive - UI 4046 - 2014
grant.Provided by PTCRIS: 137276
UI 4046-2014
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2012/03 - 2015/06 Characterization of ER-quality control for the F508del-CFTR protein: potential therapeutic targets for cystic fibrosis
grant.Provided by PTCRIS: 122299
PTDC/SAU-GMG/122299/2010
Principal investigator
Stichting Weten

Fundação para a Ciência e a Tecnologia
Concluded
2014/01/01 - 2014/12/31 Diagnosis, Prognosis and Personalized Treatment of Cystic Fibrosis
MED-2013-250
Principal investigator
Gilead Sciences Lda
Concluded
2013/01 - 2014/01 Incentive - UI 4046 - 2013
info:eu-repo/grantAgreement/FCT/3599-PPCDT/132852/PT
Principal investigator
Universidade do Algarve Faculdade de Ciências e Tecnologia
Concluded
2011/01 - 2013/12 Elucidating the role of novel CFTR interactors - new mechanisms in membrane protein trafficking
grant.Provided by PTCRIS: 112635
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2011 - 2013 Identification of Novel Targets Rescuing of F508del-CFTR Traffic: Mechanism of Action
7207534
Principal investigator
Cystic Fibrosis Foundation
Concluded
2009/01 - 2012/06 Diagnosis, Prognosis and Treatment of Cystic Fibrosis
grant.Provided by PTCRIS: 83103
PIC/IC/83103/2007
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2007 - 2011 TargetScreen2 - Novel post-genomics cell-based screens for drug targeting in membrane protein disorders
FP6-2005-LH-7-037365
Principal investigator
European Union
Concluded
2006 - 2010 EuroCareCF - European Coordination Action for Research in Cystic Fibrosis
FP6-2004-LSH-018932
Researcher
European Union
Concluded
2007/11 - 2009/04 A novel insight into CFTR Phosphorylation: implications for its processing, trafficking and function
grant.Provided by PTCRIS: 67058
Researcher
Fundação para a Ciência e a Tecnologia
2005 - 2008 NEUPROCF - Development of New Technologies for Low Abundance Proteomics: Application to Cystic Fibrosis
FP6-2003-LSH-512044
Researcher
European Union
Concluded
2005 - 2008 Use of Human-Murine CFTR Chimeras to Investigate the Coupling of Permeation and Gating in the CFTR Chloride Channel
BB/C517517/1
Researcher
Biotechnology and Biological Sciences Research Council
Concluded
2005 - 2008 SNiP2CHIP - Development of a complete integrated SNP analysis system
FP6-2004-IST-NMP-2-016833
Researcher
European Union
Concluded
2005/07 - 2007/06 CFTR Interactome
grant.Provided by PTCRIS: 58425
SAU/MMO/58425/2004
grant.Provided by PTCRIS: 82407
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2005/06 - 2007/06 Caenorhabditis elegans as a model to study folding of the CFTR protein
grant.Provided by PTCRIS: 56609
BIA-BCM/56609/2004
grant.Provided by PTCRIS: 82375
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2005 - 2007 Aquisition of a small physiology unit
no ref
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2003/02/01 - 2006 Folding, Processing and Function of Normal and Mutant Cystic Fibrosis Transmembranar Conductance Regulator: Structural Implications
MGI/47382/2002
 Fundação para a Ciência e a Tecnologia
Concluded
2002/05/15 - 2005 In search of new molecular targets for the development of novel therapeutic strategies for cystic fibrosis
MGI/40878/2001
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2002 - 2005 CF-Chip - Novel Genechip Technology for Early Detection of Cystic Fibrosis
FP5-QLRT-2000-0182
Researcher
European Union
Concluded
2001 - 2004 Biogenesis & Function of CFTR Protein with Different Mutations: Molecular Basis for Clinical and Therapeutic of Cystic Fibrosis
MGI/35737/1999
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
2000 - 2004 CF Network - Thematic Network around Cystic Fibrosis and Related Diseases
FP5-QLK-1999-00241
Researcher
European Union
Concluded
1997 - 2000 Cystic Fibrosis - Traffic and Cellular Function of CFTR
PSAU/P/SAU/55/96
Principal investigator
Fundação para a Ciência e a Tecnologia
Concluded
1995 - 1997 Expression Studies of CFTR Gene
PBIC/C/BIA/2060/95
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
1995 - 1997 Immortalization of Epithelial Cells in Portuguese Cystic Fibrosis Patients: Models to Study CFTR Gene Expression
049 C0
Principal investigator
Ambassade de France au Portugal
Concluded
1993 - 1995 Molecular Biology of Cystic Fibrosis in the Portuguese Population: Epidemiology, Anthropogenetics and Physiopathology
PBIC/C/SAU/1587/92
Researcher
Fundação para a Ciência e a Tecnologia
Concluded

Other

Designation Funders
2010 - 2014 COST Action - Microbial Cell Surface Determinants of Virulence as Targets for New Therapeutics in CF
BM1003
Researcher
European Union
Concluded
Outputs

Publications

Book
  1. Freitas, P.P.; Ferreira, H.A.; Graham, D.L.; Clarke, L.A.; Amaral, M.D.; Martins, V.; Fonseca, L.; Cabral, J.S.. Magnetoresistive DNA chips. 2004.
    10.1016/B978-012088487-2/50008-X
Conference paper
  1. Barros, Patricia; Amaral, Andreia; Abrantes, Leonor; Oliveira, Tiago; Louro, Henriqueta; Silva, Maria Joâo; Jordan, Peter; Gama Carvalho, Margarida; Matos, Paulo. "Upregulation of RAC1/PAK1 signalling promotes DNA damage repair in colorectal cancer cells". 2018.
  2. Barros, Patrícia; Amaral, Andreia; Abrantes, Leonor; Oliveira, Tiago; Lourio, Henriqueta; Silva, Maria João; Jordan, Peter; Gama-Carvalho, Margarida; Matos, Paulo. "Activation of RAC1/PAK1 axis potentiates transcriptional upregulation of DNA damage response genes via the BCL6/STAT5 switch". 2017.
  3. Barros, Patrícia; Amaral, Andreia; Abrantes, Leonor; Oliveira, Tiago; Louro, Henriqueta; Silva, Maria João; Jordan, Peter; et al. "Stimulation of RAC1/PAK1 signalling upregulates DNA damage repair genes via STAT5 stimulation of BCL6 repressed loci". 2017.
  4. Matos, Paulo; Moniz, Sónia; Moraes, Bruno; Mendes, Ana Isabel; Amaral, Margarida D.; Jordan, Peter. "Stimulation of Rac1 signalling enhances apical retention of chemically corrected F508del-CFTR in human airway cells.". 2012.
  5. Lagae, L.; Wirix-Speetjens, R.; Liu, C.-X.; Laureyn, W.; Borghs, G.; Harvey, S.; Galvin, P.; et al. "Magnetic biosensors for genetic screening of cystic fibrosis". 2005.
    10.1049/ip-cds:20050022
  6. Freitas, P.P.; Ferreira, H.; Graham, D.; Feliciano, N.; Carias, C.; Ferreira, R.; Clarke, L.; et al. "Spintronic biosensors for gene or micro-organism detection". 2005.
  7. Ferreira, H.A.; Graham, D.L.; Feliciano, N.; Clarke, L.A.; Amaral, M.D.; Freitas, P.P.. "Detection of cystic fibrosis related DNA targets using AC field focusing of magnetic labels and spin-valve sensors". 2005.
    10.1109/TMAG.2005.855340
Journal article
  1. Marianna Pócsi; Zsolt Fejes; Zsolt Bene; Attila Nagy; István Balogh; Margarida D. Amaral; Milan Macek, Jr.; Béla Nagy, Jr.. "Human epididymis protein 4 (HE4) plasma concentration inversely correlates with the improvement of cystic fibrosis lung disease in p.Phe508del-CFTR homozygous cases treated with the CFTR modulator lumacaftor/ivacaftor combination". Journal of Cystic Fibrosis (2023): https://doi.org/10.1016/j.jcf.2023.04.001.
    10.1016/j.jcf.2023.04.001
  2. Margarida D. Amaral. "Using the genome to correct the ion transport defect in cystic fibrosis". The Journal of Physiology (2023): https://doi.org/10.1113/JP282308.
    10.1113/JP282308
  3. Railean V; Rodrigues CS; Ramalho SS; Silva IAL; Bartosch J; Farinha CM; Ines Pankonien; Amaral MD. "Personalized medicine: Function of CFTR variant p.Arg334Trp is rescued by currently available CFTR modulators.". Frontiers in molecular biosciences (2023): https://europepmc.org/articles/PMC10063961.
    10.3389/fmolb.2023.1155705
  4. Santos L; Nascimento R; Duarte A; Railean V; Amaral MD; Patrick Harrison; Gama-Carvalho M; Carlos Farinha. "Mutation-class dependent signatures outweigh disease-associated processes in cystic fibrosis cells.". Cell & bioscience (2023): https://europepmc.org/articles/PMC9912517.
    10.1186/s13578-023-00975-y
  5. Luka A. Clarke; Margarida D. Amaral. "What Can RNA-Based Therapy Do for Monogenic Diseases?". Pharmaceutics (2023): https://doi.org/10.3390/pharmaceutics15010260.
    10.3390/pharmaceutics15010260
  6. Mafalda Bacalhau; Filipa C. Ferreira; Iris A. L. Silva; Camilla D. Buarque; Margarida D. Amaral; Miquéias Lopes-Pacheco. "Additive Potentiation of R334W-CFTR Function by Novel Small Molecules". Journal of Personalized Medicine (2023): https://doi.org/10.3390/jpm13010102.
    10.3390/jpm13010102
  7. MD Amaral; Bacalhau M; Ferreira FC; Kmit A; Souza FR; da Silva VD; Pimentel AS; et al. "Identification of Novel F508del-CFTR traffic correctors among triazole derivatives.". European journal of pharmacology (2022): https://doi.org/10.1016/j.ejphar.2022.175396.
    10.1016/j.ejphar.2022.175396
  8. MD Amaral; Jo S; Centeio R; Park J; Ousingsawat J; Jeon DK; Talbi K; et al. "The SLC26A9 inhibitor S9-A13 provides no evidence for a role of SLC26A9 in airway chloride secretion but suggests a contribution to regulation of ASL pH and gastric proton secretion.". FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2022): https://doi.org/10.1096/fj.202200313RR.
    10.1096/fj.202200313rr
  9. Amaral MD; Harrison PT. "Development of novel therapeutics for all individuals with CF (the future goes on).". Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society (2022): https://doi.org/10.1016/j.jcf.2022.10.007.
    10.1016/j.jcf.2022.10.007
  10. Lisa Rodenburg; Livia Delpiano; Violeta Railean; Raquel Centeio; Madalena Pinto; Shannon M. A. Smits; Isabelle S. van der Windt; et al. "Drug Repurposing for Cystic Fibrosis: Identification of Drugs That Induce CFTR-Independent Fluid Secretion in Nasal Organoids". International Journal of Molecular Sciences (2022): https://www.mdpi.com/1422-0067/23/20/12657.
    10.3390/ijms232012657
  11. Margarida C Quaresma; Hugo M Botelho; Ines Pankonien; Cláudia S Rodrigues; Madalena C Pinto; Pau R Costa; Aires Duarte; Margarida D Amaral. "Exploring YAP1-centered networks linking dysfunctional CFTR to epithelial–mesenchymal transition". Life Science Alliance (2022): https://doi.org/10.26508/lsa.202101326.
    10.26508/lsa.202101326
  12. Gimano Amatngalim; Lisa Rodenburg; Aalbers BL; Henriette Dreyer; Aarts EM; Sarhane D; Spelier S; et al. "Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epithelia.". Life science alliance (2022): https://europepmc.org/articles/PMC9351388.
    10.26508/lsa.202101320
  13. João F. Ferreira; Iris A. L. Silva; Hugo M. Botelho; Margarida D. Amaral; Carlos M. Farinha. "Absence of EPAC1 Signaling to Stabilize CFTR in Intestinal Organoids". Cells (2022): https://doi.org/10.3390/cells11152295.
    10.3390/cells11152295
  14. Margarida D. Amaral. "Precision medicine for rare diseases: The times they are A-Changin'". Current Opinion in Pharmacology 63 (2022): 102201-102201. https://doi.org/10.1016/j.coph.2022.102201.
    10.1016/j.coph.2022.102201
  15. "Systems Approaches to Unravel Molecular Function: High-content siRNA Screen Identifies TMEM16A Traffic Regulators as Potential Drug Targets for Cystic Fibrosis". Journal of Molecular Biology 434 5 (2022): 167436-167436. http://dx.doi.org/10.1016/j.jmb.2021.167436.
    10.1016/j.jmb.2021.167436
  16. Ines Pankonien; Margarida C. Quaresma; Cláudia S. Rodrigues; Margarida D. Amaral. "CFTR, Cell Junctions and the Cytoskeleton". International Journal of Molecular Sciences (2022): https://doi.org/10.3390/ijms23052688.
    10.3390/ijms23052688
  17. "CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system". Molecular Systems Biology 18 2 (2022): http://dx.doi.org/10.15252/msb.202110629.
    10.15252/msb.202110629
  18. "Rescue of Mutant CFTR Trafficking Defect by the Investigational Compound MCG1516A". Cells 11 1 (2022): 136-136. http://dx.doi.org/10.3390/cells11010136.
    10.3390/cells11010136
  19. "Rare Trafficking CFTR Mutations Involve Distinct Cellular Retention Machineries and Require Different Rescuing Strategies". International Journal of Molecular Sciences 23 1 (2021): 24-24. http://dx.doi.org/10.3390/ijms23010024.
    10.3390/ijms23010024
  20. Madalena C. Pinto; Margarida C. Quaresma; Iris A. L. Silva; Violeta Railean; Sofia S. Ramalho; Margarida D. Amaral. "Synergy in Cystic Fibrosis Therapies: Targeting SLC26A9". International Journal of Molecular Sciences (2021): https://doi.org/10.3390/ijms222313064.
    10.3390/ijms222313064
  21. "Integrity and Stability of PTC Bearing CFTR mRNA and Relevance to Future Modulator Therapies in Cystic Fibrosis". Genes 12 11 (2021): 1810-1810. http://dx.doi.org/10.3390/genes12111810.
    10.3390/genes12111810
  22. "CyFi-MAP: an interactive pathway-based resource for cystic fibrosis.". Scientific reports (2021): https://europepmc.org/articles/PMC8592983.
    10.1038/s41598-021-01618-3
  23. Filipa B. Simões; Arthur Kmit; Margarida D. Amaral. "Cross-talk of inflammatory mediators and airway epithelium reveals the cystic fibrosis transmembrane conductance regulator as a major target". ERJ Open Research (2021): 00247-2021. https://doi.org/10.1183/23120541.00247-2021.
    10.1183/23120541.00247-2021
  24. Juliana Roda; Teresa Teixeira; Iris AI Silva; Teresa Reis Silva; Ricardo Ferreira; Margarida D. Amaral; Guiomar Oliveira. "Pediatric population with cystic fibrosis in the centre of Portugal: candidates for new therapies". Jornal de Pediatria (2021): https://doi.org/10.1016/j.jped.2021.05.010.
    10.1016/j.jped.2021.05.010
  25. "Pharmacological Modulation of Ion Channels for the Treatment of Cystic Fibrosis". Journal of Experimental Pharmacology Volume 13 (2021): 693-723. http://dx.doi.org/10.2147/jep.s255377.
    10.2147/jep.s255377
  26. "Personalized Medicine Based on Nasal Epithelial Cells: Comparative Studies with Rectal Biopsies and Intestinal Organoids". Journal of Personalized Medicine 11 5 (2021): 421-421. http://dx.doi.org/10.3390/jpm11050421.
    10.3390/jpm11050421
  27. "Enhanced Expression of Human Epididymis Protein 4 (HE4) Reflecting Pro-Inflammatory Status Is Regulated by CFTR in Cystic Fibrosis Bronchial Epithelial Cells". Frontiers in Pharmacology 12 (2021): http://dx.doi.org/10.3389/fphar.2021.592184.
    10.3389/fphar.2021.592184
  28. Margarida D. Amaral. "How to determine the mechanism of action of CFTR modulator compounds: A gateway to theranostics". European Journal of Medicinal Chemistry 210 (2021): 112989-112989. https://doi.org/10.1016/j.ejmech.2020.112989.
    10.1016/j.ejmech.2020.112989
  29. Marne C Hagemeijer; Annelotte M Vonk; Nikhil T Awatade; Iris A L Silva; Christian Tischer; Volker Hilsenstein; Jeffrey M Beekman; et al. "An open-source high-content analysis workflow for CFTR function measurements using the forskolin-induced swelling assay". Bioinformatics (2020): https://doi.org/10.1093/bioinformatics/btaa1073.
    10.1093/bioinformatics/btaa1073
  30. "Assessment of Distinct Electrophysiological Parameters in Rectal Biopsies for the Choice of the Best Diagnosis/Prognosis Biomarkers for Cystic Fibrosis.". Frontiers in physiology (2020): https://europepmc.org/articles/PMC7786280.
    10.3389/fphys.2020.604580
  31. Stefano Donegà; Malgorzata Ewa Rogalska; Giulia Pianigiani; Susana Igreja; Margarida Duarte Amaral; Franco Pagani. "Rescue of common exon-skipping mutations in cystic fibrosis with modified U1 snRNAs". Human Mutation 41 12 (2020): 2143-2154. https://doi.org/10.1002/humu.24116.
    10.1002/humu.24116
  32. Margarida C. Quaresma; Ines Pankonien; Luka A. Clarke; Luís S. Sousa; Iris A. L. Silva; Violeta Railean; Tereza Doušová; Jonas Fuxe; Margarida D. Amaral. "Mutant CFTR Drives TWIST1 mediated epithelial–mesenchymal transition". Cell Death & Disease 11 10 (2020): https://doi.org/10.1038/s41419-020-03119-z.
    10.1038/s41419-020-03119-z
  33. Luís Sousa; Ines Pankonien; Filipa B. Simões; Marc Chanson; Margarida D. Amaral. "Impact of KLF4 on Cell Proliferation and Epithelial Differentiation in the Context of Cystic Fibrosis". International Journal of Molecular Sciences 21 18 (2020): 6717-6717. https://doi.org/10.3390/ijms21186717.
    10.3390/ijms21186717
  34. "Extensive CFTR sequencing through NGS in Brazilian individuals with cystic fibrosis: unravelling regional discrepancies in the country.". Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society (2020): https://doi.org/10.1016/j.jcf.2020.08.007.
    10.1016/j.jcf.2020.08.007
  35. "Organoids as a personalized medicine tool for ultra-rare mutations in cystic fibrosis: The case of S955P and 1717-2A>G.". Biochimica et biophysica acta. Molecular basis of disease (2020): https://doi.org/10.1016/j.bbadis.2020.165905.
    10.1016/j.bbadis.2020.165905
  36. "Characterization of the mechanism of action of RDR01752, a novel corrector of F508del-CFTR.". Biochemical pharmacology (2020): https://doi.org/10.1016/j.bcp.2020.114133.
    10.1016/j.bcp.2020.114133
  37. Luis Sousa; Ines Pankonien; Luka A Clarke; Iris Silva; Karl Kunzelmann; Margarida D Amaral. "KLF4 Acts as a wt-CFTR Suppressor through an AKT-Mediated Pathway". Cells 9 7 (2020): 1607-1607. https://doi.org/10.3390/cells9071607.
    10.3390/cells9071607
  38. "Cytoskeleton regulators CAPZA2 and INF2 associate with CFTR to control its plasma membrane levels under EPAC1 activation.". The Biochemical journal (2020): https://doi.org/10.1042/BCJ20200287.
    10.1042/bcj20200287
  39. Inna Uliyakina; Hugo M. Botelho; Ana C. da Paula; Sara Afonso; Miguel J. Lobo; Verónica Felício; Carlos M. Farinha; Margarida D. Amaral. "Full Rescue of F508del-CFTR Processing and Function by CFTR Modulators Can Be Achieved by Removal of Two Regulatory Regions". International Journal of Molecular Sciences 21 12 (2020): 4524-4524. https://doi.org/10.3390/ijms21124524.
    10.3390/ijms21124524
  40. "Cystic fibrosis drug trial design in the era of CFTR modulators associated with substantial clinical benefit: stakeholders' consensus view.". Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society (2020): https://doi.org/10.1016/j.jcf.2020.05.012.
    10.1016/j.jcf.2020.05.012
  41. "Laboratory biomarkers for lung disease severity and progression in cystic fibrosis.". Clinica chimica acta; international journal of clinical chemistry (2020): https://doi.org/10.1016/j.cca.2020.05.015.
    10.1016/j.cca.2020.05.015
  42. Madalena C. Pinto; Rainer Schreiber; Joana Lerias; Jiraporn Ousingsawat; Aires Duarte; Margarida Amaral; Karl Kunzelmann. "Regulation of TMEM16A by CK2 and Its Role in Cellular Proliferation". Cells 9 5 (2020): 1138-1138. https://doi.org/10.3390/cells9051138.
    10.3390/cells9051138
  43. "Letter to the editor of JCF.". Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society (2020): https://doi.org/10.1016/j.jcf.2020.02.011.
    10.1016/j.jcf.2020.02.011
  44. Amaral, Margarida. "What Role Does CFTR Play in Development, Differentiation, Regeneration and Cancer?". International Journal of Molecular Sciences 21 9 (2020): 3133. https://www.mdpi.com/1422-0067/21/9/3133/htm.
    Open access • Published • 10.3390/ijms21093133
  45. Celso Almeida; Margarida D. Amaral. "A central role of the endoplasmic reticulum in the cell emerges from its functional contact sites with multiple organelles". Cellular and Molecular Life Sciences (2020): https://doi.org/10.1007/s00018-020-03523-w.
    10.1007/s00018-020-03523-w
  46. Amaral, Margarida. "Protocol for application, standardization and validation of the forskolin induced swelling assay in Cystic Fibrosis human colon organoids". STAR Protocols (2020): https://star-protocols.cell.com/protocols/72.
    Open access • In press • 10.1016/j.xpro.2020.100019
  47. Margarida D. Amaral; Jeffrey M. Beekman. "Activating alternative chloride channels to treat CF: Friends or Foes?". Journal of Cystic Fibrosis (2020): https://doi.org/10.1016/j.jcf.2019.10.005.
    10.1016/j.jcf.2019.10.005
  48. Amaral, M.D.; Hutt, D.M.; Tomati, V.; Botelho, H.M.; Pedemonte, N.. "CFTR processing, trafficking and interactions". Journal of Cystic Fibrosis 19 (2020): S33-S36. http://www.scopus.com/inward/record.url?eid=2-s2.0-85074130058&partnerID=MN8TOARS.
    10.1016/j.jcf.2019.10.017
  49. Davies JC; Drevinek P; Elborn JS; Kerem E; Lee T. "Speeding up access to new drugs for CF: Considerations for clinical trial design and delivery.". Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society (2019): http://europepmc.org/abstract/med/31303382.
    10.1016/j.jcf.2019.06.011
  50. Amaral MD; de Boeck K; ECFS Strategic Planning Task Force on ‘Speeding up access to new drugs for CF’. "Theranostics by testing CFTR modulators in patient-derived materials: The current status and a proposal for subjects with rare CFTR mutations.". Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society (2019): http://europepmc.org/abstract/med/31326274.
    10.1016/j.jcf.2019.06.010
  51. Matos AM; Pinto FR; Barros P; Amaral MD; Pepperkok R; Matos P; Matos, A.M.; et al. "Inhibition of calpain 1 restores plasma membrane stability to pharmacologically rescued Phe508del-CFTR variant.". The Journal of biological chemistry 294 36 (2019): 13396-13410. http://europepmc.org/abstract/med/31324722.
    10.1074/jbc.ra119.008738
  52. João D. Santos; Sara Canato; Ana S. Carvalho; Hugo M. Botelho; Kerman Aloria; MD Amaral; Rune Matthiesen; et al. "Folding Status Is Determinant over Traffic-Competence in Defining CFTR Interactors in the Endoplasmic Reticulum". Cells (2019): https://www.mdpi.com/2073-4409/8/4/353.
    10.3390/cells8040353
  53. Kmit A; Marson FAL; Pereira SV; Vinagre AM; Leite GS; Servidoni MF; Ribeiro JD; et al. "Extent of rescue of F508del-CFTR function by VX-809 and VX-770 in human nasal epithelial cells correlates with SNP rs7512462 in SLC26A9 gene in F508del/F508del Cystic Fibrosis patients.". Biochimica et biophysica acta. Molecular basis of disease (2019): http://europepmc.org/abstract/med/30716472.
    10.1016/j.bbadis.2019.01.029
  54. Simões, F.B.; Quaresma, M.C.; Clarke, L.A.; Silva, I.A.L.; Pankonien, I.; Railean, V.; Kmit, A.; et al. "TMEM16A chloride channel does not drive mucus production". Life Science Alliance 2 6 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85075115008&partnerID=MN8TOARS.
    10.26508/LSA.201900462
  55. Patel W; Moore PJ; Sassano MF; Lopes-Pacheco M; Aleksandrov AA; Amaral MD; Tarran R; Gray MA. "Increases in cytosolic Ca2+ induce dynamin- and calcineurin-dependent internalisation of CFTR.". Cellular and molecular life sciences : CMLS (2018): http://europepmc.org/abstract/med/30547226.
    10.1007/s00018-018-2989-3
  56. Amaral, Margarida; Marcão, Ana; Barreto, Celeste; Pereira, Luísa; Vaz, Luísa; Cavaco, José; Casimiro, Ana; et al. "Cystic Fibrosis Newborn Screening in Portugal: PAP Value in Populations with Stringent Rules for Genetic Studies". International Journal of Neonatal Screening (2018): http://www.mdpi.com/2409-515X/4/3/22.
    10.3390/ijns4030022
  57. Joana Lérias; Madalena Pinto; Roberta Benedetto; Rainer Schreiber; Margarida Amaral; Massimo Aureli; Karl Kunzelmann. "Compartmentalized crosstalk of CFTR and TMEM16A (ANO1) through EPAC1 and ADCY1". Cellular Signalling 44 (2018): 10-19. https://doi.org/10.1016/j.cellsig.2018.01.008.
    10.1016/j.cellsig.2018.01.008
  58. Liu J; Bihler H; Farinha CM; Awatade NT; Romão AM; Mercadante D; Cheng Y; et al. "Partial rescue of F508del-cystic fibrosis transmembrane conductance regulator channel gating with modest improvement of protein processing, but not stability, by a dual-acting small molecule.". British Journal of Pharmacology (2018): http://europepmc.org/abstract/med/29318594.
    10.1111/bph.14141
  59. Olivença DV; Uliyakina I; Fonseca LL; Amaral MD; Voit EO; Pinto FR; Voit E. "A Mathematical Model of the Phosphoinositide Pathway.". Scientific reports (2018): http://europepmc.org/abstract/med/29500467.
    10.1038/s41598-018-22226-8
  60. Lérias JR; Pinto MC; Botelho HM; Awatade NT; Quaresma MC; Silva IAL; Wanitchakool P; et al. "A novel microscopy-based assay identifies extended synaptotagmin-1 (ESYT1) as a positive regulator of anoctamin 1 traffic.". Biochimica et biophysica acta (2018): http://europepmc.org/abstract/med/29154949.
    10.1016/j.bbamcr.2017.11.009
  61. Clarke, L. A.; Awatade, N. T.; Felicio, V. M.; Silva, I. A.; Calucho, M.; Pereira, L.; Azevedo, P.; et al. "The effect of PTC mutations on CFTR mRNA abundance in human nasal epithelium and intestinal organoids: A basis for read-through therapies in Cystic Fibrosis". Hum Mutat (2018): https://www.ncbi.nlm.nih.gov/pubmed/30488522.
    10.1002/humu.23692
  62. Awatade, N. T.; Ramalho, S.; Silva, I. A. L.; Felício, V.; Botelho, H. M.; de Poel, E.; Vonk, A.; et al. "R560S: A class II CFTR mutation that is not rescued by current modulators". J Cyst Fibros (2018): https://www.ncbi.nlm.nih.gov/pubmed/30030066.
    10.1016/j.jcf.2018.07.001
  63. Canato, S.; Santos, J. D.; Carvalho, A. S.; Aloria, K.; Amaral, M. D.; Matthiesen, R.; Falcao, A. O.; Farinha, C. M.. "Proteomic interaction profiling reveals KIFC1 as a factor involved in early targeting of F508del-CFTR to degradation". Cell Mol Life Sci (2018): https://www.ncbi.nlm.nih.gov/pubmed/30066085.
    10.1007/s00018-018-2896-7
  64. Matos, A. M.; Gomes-Duarte, A.; Faria, M.; Barros, P.; Jordan, P.; Amaral, M. D.; Matos, P.; et al. "Prolonged co-treatment with HGF sustains epithelial integrity and improves pharmacological rescue of Phe508del-CFTR". Sci Rep 8 1 (2018): 13026-13026. https://www.ncbi.nlm.nih.gov/pubmed/30158635.
    10.1038/s41598-018-31514-2
  65. Nagy, B.; Bene, Z.; Fejes, Z.; Heltshe, S. L.; Reid, D.; Ronan, N. J.; McCarthy, Y.; et al. "Human epididymis protein 4 (HE4) levels inversely correlate with lung function improvement (delta FEV". J Cyst Fibros (2018): https://www.ncbi.nlm.nih.gov/pubmed/30268371.
    10.1016/j.jcf.2018.08.013
  66. Amaral, M. D.; Boj, S. F.; Shaw, J.; Leipziger, J.; Beekman, J. M.. "Cystic fibrosis: Beyond the airways. Report on the meeting of the basic science working group in Loutraki, Greece". J Cyst Fibros 17 4 (2018): 441-443. https://www.ncbi.nlm.nih.gov/pubmed/29866530.
    10.1016/j.jcf.2018.05.008
  67. Clancy, J. P.; Cotton, C. U.; Donaldson, S. H.; Solomon, G. M.; VanDevanter, D. R.; Boyle, M. P.; Gentzsch, M.; et al. "CFTR modulator theratyping: Current status, gaps and future directions". J Cyst Fibros (2018): https://www.ncbi.nlm.nih.gov/pubmed/29934203.
    10.1016/j.jcf.2018.05.004
  68. Roberta Benedetto; Jiraporn Ousingsawat; Podchanart Wanitchakool; Yong Zhang; Michael J. Holtzman; Margarida Amaral; Jason R. Rock; Rainer Schreiber; Karl Kunzelmann. "Epithelial Chloride Transport by CFTR Requires TMEM16A". Scientific Reports (2017): https://doi.org/10.1038/s41598-017-10910-0.
    10.1038/s41598-017-10910-0
  69. Beekman, Jeffrey M; Wang, Chiuhui Mary; Casati, Sara; Tuggle, Katherine L; Gulmans, Vincent A M; Amaral, Margarida; De Boeck, Kris. "Biobanking: towards increased access of biomaterials in cystic fibrosis. Report on the pre-conference meeting to the 13th ECFS Basic Science Conference, Pisa, 30 March-2 April, 2016.". Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society (2017): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:28478961&KeyUID=MEDLINE:28478961.
    10.1016/j.jcf.2017.04.009
  70. Bosch, Barbara; Bilton, Diana; Sosnay, Patrick; Raraigh, Karen S; Mak, Denise Y F; Ishiguro, Hiroshi; Gulmans, Vincent; et al. "Ethnicity impacts the cystic fibrosis diagnosis: A note of caution.". Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society (2017): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:28233695&KeyUID=MEDLINE:28233695.
    10.1016/j.jcf.2017.01.016
  71. Servidoni, M.F.; Gomez, C.C.S.; Marson, F.A.L.; Toro, A.A.D.C.; Ribeiro, M.Â.G.O.; Ribeiro, J.D.; Ribeiro, A.F.; et al. "Sweat test and cystic fibrosis: Overview of test performance at public and private centers in the state of São Paulo, Brazil,Teste do suor e fibrose cística: Panorama da realização do teste em centros públicos e privados do estado de São Paulo". Jornal Brasileiro de Pneumologia 43 2 (2017): 121-128. http://www.scopus.com/inward/record.url?eid=2-s2.0-85019951330&partnerID=MN8TOARS.
    10.1590/S1806-37562016000000076
  72. Miguel J. Lobo; Margarida D. Amaral; Manuela Zaccolo; Carlos M. Farinha. "EPAC1 activation by cAMP stabilizes CFTR at the membrane by promoting its interaction with NHERF1". Journal of Cell Science 129 13 (2016): 2599-2612. https://doi.org/10.1242/jcs.185629.
    10.1242/jcs.185629
  73. De Boeck K; Amaral MD. "Classification of CFTR mutation classes - Authors' reply.". (2016): http://europepmc.org/abstract/med/27377413.
    10.1016/S2213-2600(16)30189-8
  74. Pereira JF; Awatade NT; Loureiro CA; Matos P; Amaral MD; Jordan P; Pereira, Joana; et al. "The third dimension: new developments in cell culture models for colorectal research.". (2016): http://europepmc.org/abstract/med/27147463.
    10.1007/s00018-016-2258-2
  75. Felício V; Ramalho AS; Igreja S; Amaral MD. "mRNA-based Detection of Rare CFTR Mutations Improves Genetic Diagnosis of Cystic Fibrosis in Populations with High Genetic Heterogeneity.". (2016): http://europepmc.org/abstract/med/27174726.
    10.1111/cge.12802
  76. De Boeck K; Amaral MD. "Progress in therapies for cystic fibrosis.". (2016): http://europepmc.org/abstract/med/27053340.
    10.1016/S2213-2600(16)00023-0
  77. Nagy B; Fila L; Clarke LA; Gönczy F; Bede O; Nagy D; Újhelyi R; et al. "Human epididymis protein 4 (HE4): a novel serum inflammatory biomarker in cystic fibrosis.". (2016): http://europepmc.org/abstract/med/27105680.
    10.1016/j.chest.2016.04.006
  78. Igreja S; Clarke LA; Botelho HM; Marques L; Amaral MD. "Correction of a Cystic Fibrosis Splicing Mutation by Antisense Oligonucleotides.". (2016): http://europepmc.org/abstract/med/26553470.
    10.1002/humu.22931
  79. Amaral MD; Farinha CM; Matos P; Botelho HM. "Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR).". Methods in molecular biology (Clifton, N.J.) (2016): http://europepmc.org/abstract/med/27665554.
    10.1007/978-1-4939-3804-9_7
  80. Ramalho, Anabela S.; Clarke, Luka A.; Sousa, Marisa; Felicio, Verónica; Barreto, Celeste; Lopes, Carlos; Amaral, Margarida D.. "Comparative ex vivo, in vitro and in silico analyses of a CFTR splicing mutation: Importance of functional studies to establish disease liability of mutations". Journal of Cystic Fibrosis 15 1 (2016): 21-33. http://dx.doi.org/10.1016/j.jcf.2015.02.002.
    10.1016/j.jcf.2015.02.002
  81. Benedetto, Roberta; Sirianant, Lalida; Pankonien, Ines; Wanitchakool, Podchanart; Ousingsawat, Jiraporn; Cabrita, Ines; Schreiber, Rainer; Amaral, Margarida; Kunzelmann, Karl. "Relationship between TMEM16A/anoctamin 1 and LRRC8A". Pflugers Archiv-European Journal of Physiology 468 10 (2016): 1751-1763. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000384425500010&KeyUID=WOS:000384425500010.
    10.1007/s00424-016-1862-1
  82. Margarida D. Amaral; William E. Balch. "Hallmarks of therapeutic management of the cystic fibrosis functional landscape". Journal of Cystic Fibrosis 14 6 (2015): 687-699. https://doi.org/10.1016/j.jcf.2015.09.006.
    10.1016/j.jcf.2015.09.006
  83. Verkman AS; Edelman A; Amaral M; Mall MA; Beekman JM; Meiners T; Galietta LJ; Bear CE. "Finding new drugs to enhance anion secretion in cystic fibrosis: Toward suitable systems for better drug screening. Report on the pre-conference meeting to the 12th ECFS Basic Science Conference, Albufeira, 25-28 March 2015.". (2015): http://europepmc.org/abstract/med/26474804.
    10.1016/j.jcf.2015.10.001
  84. Farinha CM; Sousa M; Canato S; Schmidt A; Uliyakina I; Amaral MD. "Increased efficacy of VX-809 in different cellular systems results from an early stabilization effect of F508del-CFTR.". Pharmacology Research & Perspectives 3 4 (2015): e00152. https://bpspubs.onlinelibrary.wiley.com/doi/10.1002/prp2.152.
    Open access • 10.1002/prp2.152
  85. Botelho, Hugo M.; Uliyakina, Inna; Awatade, Nikhil T.; Proença, Maria C.; Tischer, Christian; Sirianant, Lalida; Kunzelmann, Karl; Pepperkok, Rainer; Amaral, Margarida D.. "Protein Traffic Disorders: an Effective High-Throughput Fluorescence Microscopy Pipeline for Drug Discovery". Scientific Reports 5 1 (2015): http://dx.doi.org/10.1038/srep09038.
    10.1038/srep09038
  86. Awatade, Nikhil T.; Uliyakina, Inna; Farinha, Carlos M.; Clarke, Luka A.; Mendes, Karina; Solé, Amparo; Pastor, Juan; Ramos, Maria Margarida; Amaral, Margarida D.. "Measurements of Functional Responses in Human Primary Lung Cells as a Basis for Personalized Therapy for Cystic Fibrosis". EBioMedicine 2 2 (2015): 147-153. http://dx.doi.org/10.1016/j.ebiom.2014.12.005.
    10.1016/j.ebiom.2014.12.005
  87. Amaral, M. D.. "Novel personalized therapies for cystic fibrosis: treating the basic defect in all patients". Journal of Internal Medicine 277 2 (2015): 155-166. http://dx.doi.org/10.1111/joim.12314.
    10.1111/joim.12314
  88. Bell, S.C.; De Boeck, K.; Amaral, M.D.. "New pharmacological approaches for cystic fibrosis: Promises, progress, pitfalls". Pharmacology and Therapeutics 145 (2015): 19-34. https://www.sciencedirect.com/science/article/abs/pii/S0163725814001223?via%3Dihub.
    10.1016/j.pharmthera.2014.06.005
  89. Clarke, L.A.; Botelho, H.M.; Sousa, L.; Falcao, A.O.; Amaral, M.D.. "Transcriptome meta-analysis reveals common differential and global gene expression profiles in cystic fibrosis and other respiratory disorders and identifies CFTR regulators". Genomics (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84938651532&partnerID=MN8TOARS.
    10.1016/j.ygeno.2015.07.005
  90. Loureiro, C.A.; Matos, A.M.; Dias-Alves, A.; Pereira, J.F.; Uliyakina, I.; Barros, P.; Amaral, M.D.; et al. "A molecular switch in the scaffold NHERF1 enables misfolded CFTR to evade the peripheral quality control checkpoint". Science Signaling 8 377 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84929667112&partnerID=MN8TOARS.
    10.1126/scisignal.aaa1580
  91. Hartl, D.; Amaral, M.. "Cystic fibrosis - From basic science to clinical benefit: A review series". Journal of Cystic Fibrosis 14 4 (2015): 415-416. http://www.scopus.com/inward/record.url?eid=2-s2.0-84937627847&partnerID=MN8TOARS.
    10.1016/j.jcf.2015.06.001
  92. Srivastava, J.K.; Awatade, N.T.; Bhat, H.R.; Kmit, A.; Mendes, K.; Ramos, M.; Amaral, M.D.; Singh, U.P.. "Pharmacological evaluation of hybrid thiazolidin-4-one-1,3,5-triazines for NF-¿B, biofilm and CFTR activity". RSC Advances 5 108 (2015): 88710-88718. http://www.scopus.com/inward/record.url?eid=2-s2.0-84945907802&partnerID=MN8TOARS.
    10.1039/c5ra09250g
  93. Xu, Zhe; Pissarra, Luísa S.; Farinha, Carlos M.; Liu, Jia; Cai, Zhiwei; Thibodeau, Patrick H.; Amaral, Margarida D.; Sheppard, David N.. "Revertant mutants modify, but do not rescue, the gating defect of the cystic fibrosis mutant G551D-CFTR". The Journal of Physiology 592 9 (2014): 1931-1947. http://dx.doi.org/10.1113/jphysiol.2014.271817.
    10.1113/jphysiol.2014.271817
  94. Luz, S.; Cihil, K.M.; Brautigan, D.L.; Amaral, M.D.; Farinha, C.M.; Swiatecka-Urban, A.. "LMTK2-mediated phosphorylation regulates CFTR endocytosis in human airway epithelial cells". Journal of Biological Chemistry 289 21 (2014): 15080-15093. http://www.scopus.com/inward/record.url?eid=2-s2.0-84901422775&partnerID=MN8TOARS.
    10.1074/jbc.M114.563742
  95. Beekman, J.M.; Sermet-Gaudelus, I.; De Boeck, K.; Gonska, T.; Derichs, N.; Mall, M.A.; Mehta, A.; et al. "CFTR functional measurements in human models for diagnosis, prognosis and personalized therapy. Report on the pre-conference meeting to the 11th ECFS Basic Science Conference, Malta, 26-29 March 2014.". Journal of Cystic Fibrosis 13 4 (2014): 363-372. http://www.scopus.com/inward/record.url?eid=2-s2.0-84902343264&partnerID=MN8TOARS.
    10.1016/j.jcf.2014.05.007
  96. Sharma, N.; Sosnay, P.R.; Ramalho, A.S.; Douville, C.; Franca, A.; Gottschalk, L.B.; Park, J.; et al. "Experimental Assessment of Splicing Variants Using Expression Minigenes and Comparison with In Silico Predictions". Human Mutation 35 10 (2014): 1249-1259. https://onlinelibrary.wiley.com/doi/10.1002/humu.22624.
    10.1002/humu.22624
  97. Masvidal, L.; Igreja, S.; Ramos, M.D.; Alvarez, A.; De Gracia, J.; Ramalho, A.; Amaral, M.D.; Larriba, S.; Casals, T.. "Assessing the residual CFTR gene expression in human nasal epithelium cells bearing CFTR splicing mutations causing cystic fibrosis". European Journal of Human Genetics 22 6 (2014): 784-791. http://www.scopus.com/inward/record.url?eid=2-s2.0-84901020910&partnerID=MN8TOARS.
    10.1038/ejhg.2013.238
  98. Amaral, Margarida. "LMTK2 Mediated Phosphorylation Regulates CFTR Endocytosis in Human Airway Epithelial Cells". (2014):
  99. Amaral, Margarida. "CFTR Functional Measurements in Human Models For Diagnosis, Prognosis and Personalised Therapy". (2014):
  100. Amaral MD; Farinha CM. "Rescuing mutant CFTR: a multi-task approach to a better outcome in treating cystic fibrosis.". (2013): http://europepmc.org/abstract/med/23331027.
    10.2174/13816128113199990318
  101. De Boeck, K.; Kent, L.; Davies, J.; Derichs, N.; Amaral, M.; Rowe, S.M.; Middleton, P.; et al. "CFTR biomarkers: Time for promotion to surrogate end-point?". European Respiratory Journal 41 1 (2013): 203-216. http://www.scopus.com/inward/record.url?eid=2-s2.0-84872190665&partnerID=MN8TOARS.
    10.1183/09031936.00057512
  102. Tosoni, K.; Stobbart, M.; Cassidy, D.M.; Venerando, A.; Pagano, M.A.; Luz, S.; Amaral, M.D.; et al. "CFTR mutations altering CFTR fragmentation". Biochemical Journal 449 1 (2013): 295-305. http://www.scopus.com/inward/record.url?eid=2-s2.0-84870934566&partnerID=MN8TOARS.
    10.1042/BJ20121240
  103. Farinha, C.M.; Matos, P.; Amaral, M.D.. "Control of cystic fibrosis transmembrane conductance regulator membrane trafficking: Not just from the endoplasmic reticulum to the Golgi". FEBS Journal 280 18 (2013): 4396-4406. http://www.scopus.com/inward/record.url?eid=2-s2.0-84883465161&partnerID=MN8TOARS.
    10.1111/febs.12392
  104. Tian, Y.; Schreiber, R.; Wanitchakool, P.; Kongsuphol, P.; Sousa, M.; Uliyakina, I.; Palma, M.; et al. "Control of TMEM16A by INO-4995 and other inositolphosphates". British Journal of Pharmacology 168 1 (2013): 253-265. http://www.scopus.com/inward/record.url?eid=2-s2.0-84872858136&partnerID=MN8TOARS.
    10.1111/j.1476-5381.2012.02193.x
  105. Sosnay, P.R.; Siklosi, K.R.; Van Goor, F.; Kaniecki, K.; Yu, H.; Sharma, N.; Ramalho, A.S.; et al. "Defining the disease liability of variants in the cystic fibrosis transmembrane conductance regulator gene". Nature Genetics 45 10 (2013): 1160-1167. http://www.scopus.com/inward/record.url?eid=2-s2.0-84885022205&partnerID=MN8TOARS.
    10.1038/ng.2745
  106. Moniz, S.; Sousa, M.; Moraes, B.J.; Mendes, A.I.; Palma, M.; Barreto, C.; Fragata, J.I.; Amaral, M.D.; Matos, P.. "HGF stimulation of Rac1 signaling enhances pharmacological correction of the most prevalent cystic fibrosis mutant F508del-CFTR". ACS Chemical Biology 8 2 (2013): 432-442. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874094442&partnerID=MN8TOARS.
    10.1021/cb300484r
  107. Clarke, Luka A; Sousa, Lisete; Barreto, Celeste; Amaral, Margarida D. "Changes in transcriptome of native nasal epithelium expressing F508del-CFTR and intersecting data from comparable studies". Respiratory Research 14 1 (2013): 38. http://dx.doi.org/10.1186/1465-9921-14-38.
    10.1186/1465-9921-14-38
  108. Silva, M.C.; Amaral, M.D.; Morimoto, R.I.. "Neuronal Reprograming of Protein Homeostasis by Calcium-Dependent Regulation of the Heat Shock Response". PLoS Genetics 9 8 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84884610586&partnerID=MN8TOARS.
    10.1371/journal.pgen.1003711
  109. Amaral, M.D.; Farinha, C.M.. "Post-translational modifications of CFTR: Insight into protein trafficking and cystic fibrosis disease". FEBS Journal 280 18 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84883457537&partnerID=MN8TOARS.
    10.1111/febs.12345
  110. Servidoni, M.F.; Sousa, M.; Vinagre, A.M.; Cardoso, S.R.; Ribeiro, M.A.; Meirelles, L.R.; de Carvalho, R.B.; et al. "Rectal forceps biopsy procedure in cystic fibrosis: Technical aspects and patients perspective for clinical trials feasibility". BMC Gastroenterology 13 1 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84877844941&partnerID=MN8TOARS.
    10.1186/1471-230X-13-91
  111. Farinha, C.M.; King-Underwood, J.; Sousa, M.; Correia, A.R.; Henriques, B.J.; Roxo-Rosa, M.; Da Paula, A.C.; et al. "Revertants, low temperature, and correctors reveal the mechanism of F508del-CFTR rescue by VX-809 and suggest multiple agents for full correction". Chemistry and Biology 20 7 (2013): 943-955. http://www.scopus.com/inward/record.url?eid=2-s2.0-84880894290&partnerID=MN8TOARS.
    10.1016/j.chembiol.2013.06.004
  112. Almaça, J.; Faria, D.; Sousa, M.; Uliyakina, I.; Conrad, C.; Sirianant, L.; Clarke, L.A.; et al. "XHigh-content siRNA screen reveals global ENaC regulators and potential cystic fibrosis therapy targets". Cell 154 6 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84884266668&partnerID=MN8TOARS.
    10.1016/j.cell.2013.08.045
  113. Faria, D.; Lentze, N.; Almaça, J.; Luz, S.; Alessio, L.; Tian, Y.; Martins, J.P.; et al. "Regulation of ENaC biogenesis by the stress response protein SERP1". Pflugers Archiv European Journal of Physiology 463 6 (2012): 819-827. http://www.scopus.com/inward/record.url?eid=2-s2.0-84862615477&partnerID=MN8TOARS.
    10.1007/s00424-012-1091-1
  114. Mendes, F.; Farinha, C.M.; Felício, V.; Alves, P.C.; Vieira, I.; Amaral, M.D.. "BAG-1 stabilizes mutant F508del-CFTR in a ubiquitin-like-domain-dependent manner". Cellular Physiology and Biochemistry 30 5 (2012): 1120-1133. http://www.scopus.com/inward/record.url?eid=2-s2.0-84869203984&partnerID=MN8TOARS.
    10.1159/000343303
  115. Li, H.; Yang, W.; Mendes, F.; Amaral, M.D.; Sheppard, D.N.. "Impact of the cystic fibrosis mutation F508del-CFTR on renal cyst formation and growth". American Journal of Physiology - Renal Physiology 303 8 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84867719641&partnerID=MN8TOARS.
    10.1152/ajprenal.00130.2012
  116. Amaral MD. "Finding new medicines to fight CF: multiple steps of a success story.". (2012): http://europepmc.org/abstract/PMC/PMC3504594.
  117. Sousa, Marisa; Servidoni, Maria F.; Vinagre, Adriana M.; Ramalho, Anabela S.; Bonadia, Luciana C.; Felício, Verónica; Ribeiro, Maria A..; et al. "Measurements of CFTR-Mediated Cl- Secretion in Human Rectal Biopsies Constitute a Robust Biomarker for Cystic Fibrosis Diagnosis and Prognosis". PLoS ONE 7 10 (2012): http://hdl.handle.net/10400.18/1534.
    doi: 10.1371/journal.pone.0047708
  118. De Boeck K; Derichs N; Fajac I; de Jonge HR; Bronsveld I; Sermet I; Vermeulen F; et al. "New clinical diagnostic procedures for cystic fibrosis in Europe.". (2011): http://europepmc.org/abstract/med/21658643.
    10.1016/S1569-1993(11)60009-X
  119. Amaral, M.D.; Lukacs, G.L.. "Introduction to section III: biochemical methods to study CFTR protein.". Methods in molecular biology (Clifton, N.J.) 741 (2011): 213-218. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052305921&partnerID=MN8TOARS.
    10.1007/978-1-61779-117-8_14
  120. Amaral, M.D.. "Introduction to section III: resources for CFTR research.". Methods in molecular biology (Clifton, N.J.) 742 (2011): 281-283. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052549925&partnerID=MN8TOARS.
    10.1007/978-1-61779-120-8_17
  121. Ramachandran, S.; Clarke, L.A.; Scheetz, T.E.; Amaral, M.D.; McCray Jr., P.B.. "Microarray mRNA expression profiling to study cystic fibrosis.". Methods in molecular biology (Clifton, N.J.) 742 (2011): 193-212. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052546232&partnerID=MN8TOARS.
    10.1007/978-1-61779-120-8_12
  122. Ramalho, A.S.; Clarke, L.A.; Amaral, M.D.. "Quantification of CFTR transcripts.". Methods in molecular biology (Clifton, N.J.) 741 (2011): 115-135. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052341883&partnerID=MN8TOARS.
    10.1007/978-1-61779-117-8_9
  123. Amaral, M.D.. "Targeting CFTR: How to treat cystic fibrosis by CFTR-repairing therapies". Current Drug Targets 12 5 (2011): 683-693. http://www.scopus.com/inward/record.url?eid=2-s2.0-79953300971&partnerID=MN8TOARS.
    10.2174/138945011795378586
  124. Roth, E.K.; Hirtz, S.; Duerr, J.; Wenning, D.; Eichler, I.; Seydewitz, H.H.; Amaral, M.D.; Mall, M.A.. "The K + channel opener 1-EBIO potentiates residual function of mutant CFTR in rectal biopsies from cystic fibrosis patients". PLoS ONE 6 8 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-80052328287&partnerID=MN8TOARS.
    10.1371/journal.pone.0024445
  125. Silva, M.C.; Fox, S.; Beam, M.; Thakkar, H.; Amaral, M.D.; Morimoto, R.I.. "A genetic screening strategy identifies novel regulators of the proteostasis network". PLoS Genetics 7 12 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-84855258498&partnerID=MN8TOARS.
    10.1371/journal.pgen.1002438
  126. Mendes, A.I.; Matos, P.; Moniz, S.; Luz, S.; Amaral, M.D.; Farinha, C.M.; Jordan, P.. "Antagonistic regulation of cystic fibrosis transmembrane conductance regulator cell surface expression by protein kinases WNK4 and spleen tyrosine kinase". Molecular and Cellular Biology 31 19 (2011): 4076-4086. https://journals.asm.org/doi/full/10.1128/MCB.05152-11.
    10.1128/MCB.05152-11
  127. Luz, S.; Kongsupho, P.; Mendes, A.I.; Romeiras, F.; Sousa, M.; Schreiber, R.; Matos, P.; et al. "Contribution of casein kinase 2 and spleen tyrosine kinase to CFTR trafficking and protein kinase a-induced activity". Molecular and Cellular Biology 31 22 (2011): 4392-4404. http://www.scopus.com/inward/record.url?eid=2-s2.0-83255187903&partnerID=MN8TOARS.
    10.1128/MCB.05517-11
  128. Faria, D.; Dahiméne, S.; Alessio, L.; Scott-Ward, T.; Schreiber, R.; Kunzelmann, K.; Amaral, M.D.. "Effect of annexin A5 on CFTR: Regulated traffic or scaffolding?". Molecular Membrane Biology 28 1 (2011): 14-29. http://www.scopus.com/inward/record.url?eid=2-s2.0-78651316739&partnerID=MN8TOARS.
    10.3109/09687688.2010.506264
  129. Martins, J.R.; Kongsuphol, P.; Sammels, E.; Dahimène, S.; AlDehni, F.; Clarke, L.A.; Schreiber, R.; et al. "F508del-CFTR increases intracellular Ca 2+ signaling that causes enhanced calcium-dependent Cl - conductance in cystic fibrosis". Biochimica et Biophysica Acta - Molecular Basis of Disease 1812 11 (2011): 1385-1392. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052869614&partnerID=MN8TOARS.
    10.1016/j.bbadis.2011.08.008
  130. Almaça, J.; Dahimène, S.; Appel, N.; Conrad, C.; Kunzelmann, K.; Pepperkok, R.; Amaral, M.D.. "Functional genomics assays to study CFTR traffic and ENaC function.". Methods in molecular biology (Clifton, N.J.) 742 (2011): 249-264. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052553463&partnerID=MN8TOARS.
    10.1007/978-1-61779-120-8_15
  131. Luz, Simão; Kongsuphol, Patthara; Mendes, Ana Isabel; Romeiras, Francisco; Sousa, Marisa; Schreiber, Rainer; Matos, Paulo; et al. "The contribution of CK2 and spleen tyrosine kinase (SYK) to CFTR trafficking and PKA-induced activity". (2011): http://hdl.handle.net/10400.18/907.
  132. Rocchi, L.; Braz, C.; Cattani, S.; Ramalho, A.; Christan, S.; Edlinger, M.; Ascenzioni, F.; et al. "Escherichia coli-cloned CFTR loci relevant for human artificial chromosome therapy". Human Gene Therapy 21 9 (2010): 1077-1092. http://www.scopus.com/inward/record.url?eid=2-s2.0-77957847852&partnerID=MN8TOARS.
    10.1089/hum.2009.225
  133. Almaça, J.; Kongsuphol, P.; Hieke, B.; Ousingsawat, J.; Viollet, B.; Schreiber, R.; Amaral, M.D.; Kunzelmann, K.. "Erratum: AMPK controls epithelial Na+ channels through Nedd4-2 and causes an epithelial phenotype when mutated (Pflugers Archiv European Journal of Physiology DOI: 10.1007/s00424-009-0660-4)". Pflugers Archiv European Journal of Physiology 460 6 (2010): 1105-1106. http://www.scopus.com/inward/record.url?eid=2-s2.0-78549259325&partnerID=MN8TOARS.
    10.1007/s00424-010-0877-2
  134. Da Paula, Ana Carina; Sousa, Marisa; Xu, Zhe; Dawson, Elizabeth S.; Boyd, A. Christopher; Sheppard, David N.; Amaral, Margarida D.; et al. "Folding and rescue of a cystic fibrosis transmembrane conductance regulator trafficking mutant identified using human-murine chimeric proteins". Journal of Biological Chemistry 285 35 (2010): 27033-27044. http://hdl.handle.net/10400.18/157.
    10.1074/jbc.M110.120352
  135. Almaça, J.; Kongsuphol, P.; Hieke, B.; Ousingsawat, J.; Viollet, B.; Schreiber, R.; Amaral, M.D.; Kunzelmann, K.. "AMPK controls epithelial Na+ channels through Nedd4-2 and causes an epithelial phenotype when mutated". Pflugers Archiv European Journal of Physiology 458 4 (2009): 713-721. http://www.scopus.com/inward/record.url?eid=2-s2.0-67649865826&partnerID=MN8TOARS.
    10.1007/s00424-009-0660-4
  136. Ramalho, A.S.; Lewandowska, M.A.; Farinha, C.M.; Mendes, F.; Gonçalves, J.; Barreto, C.; Harris, A.; Amaral, M.D.. "Deletion of CFTR translation start site reveals functional isoforms of the protein in CF patients". Cellular Physiology and Biochemistry 24 5-6 (2009): 335-346. http://www.scopus.com/inward/record.url?eid=2-s2.0-70450231758&partnerID=MN8TOARS.
    10.1159/000257426
  137. Scott-Ward, T.S.; Amaral, M.D.. "Deletion of Phe508 in the first nucleotide-binding domain of the cystic fibrosis transmembrane conductance regulator increases its affinity for the heat shock cognate 70 chaperone". FEBS Journal 276 23 (2009): 7097-7109. http://www.scopus.com/inward/record.url?eid=2-s2.0-70449701828&partnerID=MN8TOARS.
    10.1111/j.1742-4658.2009.07421.x
  138. Rakonczay Jr., Z.; Hegyi, P.; Hasegawa, M.; Inoue, M.; You, J.; Iida, A.; Ignáth, I.; et al. "CFTR gene transfer to human cystic fibrosis pancreatic duct cells using a Sendai virus vector". Journal of Cellular Physiology 214 2 (2008): 442-455. http://www.scopus.com/inward/record.url?eid=2-s2.0-37349084579&partnerID=MN8TOARS.
    10.1002/jcp.21220
  139. Schmidt, A.; Hughes, L.K.; Cai, Z.; Mendes, F.; Li, H.; Sheppard, D.N.; Amaral, M.D.. "Prolonged treatment of cells with genistein modulates the expression and function of the cystic fibrosis transmembrane conductance regulator". British Journal of Pharmacology 153 6 (2008): 1311-1323. http://www.scopus.com/inward/record.url?eid=2-s2.0-38349033879&partnerID=MN8TOARS.
    10.1038/sj.bjp.0707663
  140. Bachhuber, T.; Almaça, J.; Aldehni, F.; Mehta, A.; Amaral, M.D.; Schreiber, R.; Kunzelmann, K.. "Regulation of the epithelial Na+ channel by the protein kinase CK2". Journal of Biological Chemistry 283 19 (2008): 13225-13232. http://www.scopus.com/inward/record.url?eid=2-s2.0-45149111650&partnerID=MN8TOARS.
    10.1074/jbc.M704532200
  141. Pissarra, L.S.; Farinha, C.M.; Xu, Z.; Schmidt, A.; Thibodeau, P.H.; Cai, Z.; Thomas, PhilipJ.; Sheppard, D.N.; Amaral, M.D.. "Solubilizing Mutations Used to Crystallize One CFTR Domain Attenuate the Trafficking and Channel Defects Caused by the Major Cystic Fibrosis Mutation". Chemistry and Biology 15 1 (2008): 62-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-38349050413&partnerID=MN8TOARS.
    10.1016/j.chembiol.2007.11.012
  142. Garcia SM; Casanueva MO; Silva MC; Amaral MD; Morimoto RI. "Neuronal signaling modulates protein homeostasis in Caenorhabditis elegans post-synaptic muscle cells.". (2007): http://europepmc.org/abstract/med/18006691.
    10.1101/gad.1575307
  143. Sousa, M.; Ousingsawat, J.; Seitz, R.; Puntheeranurak, S.; Regalado, A.; Schmidt, A.; Grego, T.; et al. "An extract from the medicinal plant Phyllanthus acidus and its isolated compounds induce airway chloride secretion: A potential treatment for cystic fibrosis". Molecular Pharmacology 71 1 (2007): 366-376. http://www.scopus.com/inward/record.url?eid=2-s2.0-33845908478&partnerID=MN8TOARS.
    10.1124/mol.106.025262
  144. Scott-Ward, T.S.; Cai, Z.; Dawson, E.S.; Doherty, A.; Da Paula, A.C.; Davidson, H.; Porteous, D.J.; et al. "Chimeric constructs endow the human CFTR Cl- channel with the gating behavior of murine CFTR". Proceedings of the National Academy of Sciences of the United States of America 104 41 (2007): 16365-16370. http://www.scopus.com/inward/record.url?eid=2-s2.0-36048936399&partnerID=MN8TOARS.
    10.1073/pnas.0701562104
  145. Amaral, M.D.; Kunzelmann, K.. "Molecular targeting of CFTR as a therapeutic approach to cystic fibrosis". Trends in Pharmacological Sciences 28 7 (2007): 334-341. http://www.scopus.com/inward/record.url?eid=2-s2.0-34347333381&partnerID=MN8TOARS.
    10.1016/j.tips.2007.05.004
  146. Davidson, H.; McLachlan, G.; Wilson, A.; Boyd, A.C.; Doherty, A.; MacGregor, G.; Davies, L.; et al. "Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways". American Journal of Respiratory Cell and Molecular Biology 35 1 (2006): 72-83. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745622697&partnerID=MN8TOARS.
    10.1165/rcmb.2005-0377OC
  147. Roxo-Rosa, M.; Da Costa, G.; Luider, T.M.; Scholte, B.J.; Coelho, A.V.; Amaral, M.D.; Penque, D.. "Proteomic analysis of nasal cells from cystic fibrosis patients and non-cystic fibrosis control individuals: Search for novel biomarkers of cystic fibrosis lung disease". Proteomics 6 7 (2006): 2314-2325. http://www.scopus.com/inward/record.url?eid=2-s2.0-33645698431&partnerID=MN8TOARS.
    10.1002/pmic.200500273
  148. Roxo-Rosa, M.; Xu, Z.; Schmidt, A.; Neto, M.; Cai, Z.; Soares, C.M.; Sheppard, D.H.; Amaral, M.D.. "Revertant mutants G550E and 4RK rescue cystic fibrosis mutants in the first nucleotide-binding domain of CFTR by different mechanisms". Proceedings of the National Academy of Sciences of the United States of America 103 47 (2006): 17891-17896. http://www.scopus.com/inward/record.url?eid=2-s2.0-33845197320&partnerID=MN8TOARS.
    10.1073/pnas.0608312103
  149. Amaral, M.D.. "Therapy through chaperones: Sense or antisense? Cystic fibrosis as a model disease". Journal of Inherited Metabolic Disease 29 2-3 (2006): 477-487. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745091537&partnerID=MN8TOARS.
    10.1007/s10545-006-0251-x
  150. Mendes, F.; Wakefield, J.; Bachhuber, T.; Barroso, M.; Bebok, Z.; Penque, D.; Kunzelmann, K.; Amaral, M.D.. "Establishment and characterization of a novel polarized MDCK epithelial cellular model for CFTR studies". Cellular Physiology and Biochemistry 16 4-6 (2005): 281-290. http://www.scopus.com/inward/record.url?eid=2-s2.0-27844525431&partnerID=MN8TOARS.
    10.1159/000089857
  151. Graham, D.L.; Ferreira, H.A.; Feliciano, N.; Freitas, P.P.; Clarke, L.A.; Amaral, M.D.. "Magnetic field-assisted DNA hybridisation and simultaneous detection using micron-sized spin-valve sensors and magnetic nanoparticles". Sensors and Actuators, B: Chemical 107 2 (2005): 936-944. http://www.scopus.com/inward/record.url?eid=2-s2.0-18544368501&partnerID=MN8TOARS.
    10.1016/j.snb.2004.12.071
  152. Farinha, C.M.; Amaral, M.D.. "Most F508del-CFTR is targeted to degradation at an early folding checkpoint and independently of calnexin". Molecular and Cellular Biology 25 12 (2005): 5242-5252. http://www.scopus.com/inward/record.url?eid=2-s2.0-20344378216&partnerID=MN8TOARS.
    10.1128/MCB.25.12.5242-5252.2005
  153. Amaral, M.D.. "Processing of CFTR: Traversing the cellular maze - How much CFTR needs to go through to avoid cystic fibrosis?". Pediatric Pulmonology 39 6 (2005): 479-491. http://www.scopus.com/inward/record.url?eid=2-s2.0-18844384936&partnerID=MN8TOARS.
    10.1002/ppul.20168
  154. Ferreira, H.A.; Feliciano, N.; Graham, D.L.; Clarke, L.A.; Amaral, M.D.; Freitas, P.P.. "Rapid DNA hybridization based on ac field focusing of magnetically labeled target DNA". Applied Physics Letters 87 1 (2005): http://www.scopus.com/inward/record.url?eid=2-s2.0-24144471217&partnerID=MN8TOARS.
    10.1063/1.1984090
  155. Englmann, A.; Clarke, L.A.; Christan, S.; Amaral, M.D.; Schindelhauer, D.; Zink, D.. "The replication timing of CFTR and adjacent genes". Chromosome Research 13 2 (2005): 183-194. http://www.scopus.com/inward/record.url?eid=2-s2.0-18744383356&partnerID=MN8TOARS.
    10.1007/s10577-005-0845-4
  156. Laner, A.; Goussard, S.; Ramalho, A.S.; Schwarz, T.; Amaral, M.D.; Courvalin, P.; Schindelhauer, D.; Grillot-Courvalin, C.. "Bacterial transfer of large functional genomic DNA into human cells". Gene Therapy 12 21 (2005): 1559-1572. http://www.scopus.com/inward/record.url?eid=2-s2.0-27544472925&partnerID=MN8TOARS.
    10.1038/sj.gt.3302576
  157. Da Paula, A.C.; Ramalho, A.S.; Farinha, C.M.; Cheung, J.; Maurisse, R.; Gruenert, D.C.; Ousingsawat, J.; Kunzelmann, K.; Amaral, M.D.. "Characterization of novel airway submucosal gland cell models for cystic fibrosis studies". Cellular Physiology and Biochemistry 15 6 (2005): 251-262. http://www.scopus.com/inward/record.url?eid=2-s2.0-22544474185&partnerID=MN8TOARS.
    10.1159/000087235
  158. Carvalho-Oliveira I; Efthymiadou A; Malhó R; Nogueira P; Tzetis M; Kanavakis E; Amaral MD; Penque D. "CFTR localization in native airway cells and cell lines expressing wild-type or F508del-CFTR by a panel of different antibodies.". (2004): http://europepmc.org/abstract/med/14729871.
    10.1177/002215540405200207
  159. Maurício AC; Penque D; Amaral MD; Ferreira KT; Mauricio, A.C.; Penque, D.; Amaral, M.D.; Ferreira, K.T.G.. "Ionic transport in tall columnar epithelial (TCE) cells obtained by nasal brushing from non-cystic fibrosis (CF) individuals.". Acta Medica Portuguesa 17 6 (2004): 427-434. http://europepmc.org/abstract/med/16197854.
  160. Ramalho, A.S.; Beck, S.; Farinha, C.M.; Clarke, L.A.; Heda, G.D.; Steiner, B.; Sanz, J.; et al. "Methods for RNA extraction, cDNA preparation and analysis of CFTR transcripts". Journal of Cystic Fibrosis 3 SUPPL. 2 (2004): 11-15. http://www.scopus.com/inward/record.url?eid=2-s2.0-19944409297&partnerID=MN8TOARS.
    10.1016/j.jcf.2004.05.004
  161. Galvin, P.; Clarke, L.A.; Harvey, S.; Amaral, M.D.. "Microarray analysis in cystic fibrosis". Journal of Cystic Fibrosis 3 SUPPL. 2 (2004): 29-33. http://www.scopus.com/inward/record.url?eid=2-s2.0-11044238806&partnerID=MN8TOARS.
    10.1016/j.jcf.2004.05.006
  162. Trezise, A.E.O.; Farinha, C.M.; Heda, G.D.; Harris, A.; Amaral, M.D.; Mouchel, N.. "Non-PCR methods for the analysis of CFTR transcripts". Journal of Cystic Fibrosis 3 SUPPL. 2 (2004): 25-28. http://www.scopus.com/inward/record.url?eid=2-s2.0-11044236594&partnerID=MN8TOARS.
    10.1016/j.jcf.2004.05.005
  163. Roxo-Rosa, M.; Davezac, N.; Bensalem, N.; Majumder, M.; Heda, G.D.; Simas, A.; Penque, D.; et al. "Proteomics techniques for cystic fibrosis research". Journal of Cystic Fibrosis 3 SUPPL. 2 (2004): 85-89. http://www.scopus.com/inward/record.url?eid=2-s2.0-19944375776&partnerID=MN8TOARS.
    10.1016/j.jcf.2004.05.019
  164. Amaral, M.D.; Clarke, L.A.; Ramalho, A.S.; Beck, S.; Broackes-Carter, F.; Rowntree, R.; Mouchel, N.; et al. "Quantitative methods for the analysis of CFTR transcripts/splicing variants". Journal of Cystic Fibrosis 3 SUPPL. 2 (2004): 17-23. http://www.scopus.com/inward/record.url?eid=2-s2.0-19944384117&partnerID=MN8TOARS.
    10.1016/j.jcf.2004.05.047
  165. Zink, D.; Amaral, M.D.; Englmann, A.; Lang, S.; Clarke, L.A.; Rudolph, C.; Alt, F.; et al. "Transcription-dependent spatial arrangements of CFTR and adjacent genes in human cell nuclei". Journal of Cell Biology 166 6 (2004): 815-825. http://www.scopus.com/inward/record.url?eid=2-s2.0-4544255977&partnerID=MN8TOARS.
    10.1083/jcb.200404107
  166. Férec, C.; Le Maréchal, C.; Audrézet, M.-P.; Farinha, C.M.; Amaral, M.D.; Gallati, S.; Sanz, J.; et al. "Analysis of genomic CFTR DNA". Journal of Cystic Fibrosis 3 SUPPL. 2 (2004): 7-10. http://www.scopus.com/inward/record.url?eid=2-s2.0-19944398107&partnerID=MN8TOARS.
    10.1016/j.jcf.2004.05.003
  167. Mendes, F.; Farinha, C.M.; Roxo-Rosa, M.; Fanen, P.; Edelman, A.; Dormer, R.; McPherson, M.; et al. "Antibodies for CFTR studies". Journal of Cystic Fibrosis 3 SUPPL. 2 (2004): 69-72. http://www.scopus.com/inward/record.url?eid=2-s2.0-19944369736&partnerID=MN8TOARS.
    10.1016/j.jcf.2004.05.016
  168. Harris, C.M.; Mendes, F.; Dragomir, A.; Doull, I.J.M.; Carvalho-Oliveira, I.; Bebok, Z.; Clancy, J.P.; et al. "Assessment of CFTR localisation in native airway epithelial cells obtained by nasal brushing". Journal of Cystic Fibrosis 3 SUPPL. 2 (2004): 43-48. http://www.scopus.com/inward/record.url?eid=2-s2.0-19944385576&partnerID=MN8TOARS.
    10.1016/j.jcf.2004.05.009
  169. Farinha, C.M.; Penque, D.; Roxo-Rosa, M.; Lukacs, G.; Dormer, R.; McPherson, M.; Pereira, M.; et al. "Biochemical methods to assess CFTR expression and membrane localization". Journal of Cystic Fibrosis 3 SUPPL. 2 (2004): 73-77. http://www.scopus.com/inward/record.url?eid=2-s2.0-19944402159&partnerID=MN8TOARS.
    10.1016/j.jcf.2004.05.017
  170. Hirtz, S.; Gonska, T.; Seydewitz, H.H.; Thomas, J.; Greiner, P.; Kuehr, J.; Brandis, M.; et al. "CFTR Cl - channel function in native human colon correlates with the genotype and phenotype in cystic fibrosis". Gastroenterology 127 4 (2004): 1085-1095. http://www.scopus.com/inward/record.url?eid=2-s2.0-5144232620&partnerID=MN8TOARS.
    10.1053/j.gastro.2004.07.006
  171. Amaral, M.D.. "Editorial". Journal of Cystic Fibrosis 3 SUPPL. 2 (2004): http://www.scopus.com/inward/record.url?eid=2-s2.0-11044229346&partnerID=MN8TOARS.
    10.1016/j.jcf.2004.05.001
  172. Edelman, A.; Amaral, M.D.. "General introduction to section C: Biochemistry and Biophysics of CFTR". Journal of Cystic Fibrosis 3 SUPPL. 2 (2004): http://www.scopus.com/inward/record.url?eid=2-s2.0-11044237150&partnerID=MN8TOARS.
    10.1016/j.jcf.2004.05.015
  173. Mendes, F.; Doucet, L.; Hinzpeter, A.; Féree, C.; Lipecka, J.; Fritsch, J.; Edelman, A.; et al. "Immunohistochemistry of CFTR in native tissues and primary epithelial cell cultures". Journal of Cystic Fibrosis 3 SUPPL. 2 (2004): 37-41. http://www.scopus.com/inward/record.url?eid=2-s2.0-19944422399&partnerID=MN8TOARS.
    10.1016/j.jcf.2004.05.008
  174. Farinha, C.M.; Mendes, F.; Roxo-Rosa, M.; Penque, D.; Amaral, M.D.. "A comparison of 14 antibodies for the biochemical detection of the cystic fibrosis transmembrane conductance regulator protein". Molecular and Cellular Probes 18 4 (2004): 235-242. http://www.scopus.com/inward/record.url?eid=2-s2.0-3242674336&partnerID=MN8TOARS.
    10.1016/j.mcp.2004.03.005
  175. Carvalho-Oliveira, I.; Efthymiadou, A.; Malhó, R.; Nogueira, P.; Tzetis, M.; Kanavakis, E.; Amaral, M.D.; Penque, D.. "CFTR Localization in Native Airway Cells and Cell Lines Expressing Wild-type or F508del-CFTR by a Panel of Different Antibodies". Journal of Histochemistry and Cytochemistry 52 2 (2004): 193-203. http://www.scopus.com/inward/record.url?eid=2-s2.0-0742322867&partnerID=MN8TOARS.
  176. Barreto, C.; Mall, M.; Amaral, M.D.; Barreto C; Mall M; Amaral MD. "Assessment of CFTR Function in Native Epithelia for the Diagnosis of Cystic Fibrosis". Pediatric Pulmonology 37 SUPPL. 26 (2004): http://www.scopus.com/inward/record.url?eid=2-s2.0-1242269343&partnerID=MN8TOARS.
  177. Amaral, M.D.; Amaral, Margarida D.. "CFTR and chaperones: Processing and degradation". Journal of Molecular Neuroscience 23 1-2 (2004): 41-48. http://www.scopus.com/inward/record.url?eid=2-s2.0-2942580461&partnerID=MN8TOARS.
    10.1385/jmn:23:1-2:041
  178. Mendes, F.; Rosa, M.R.; Dragomir, A.; Farinha, C.M.; Roomans, G.M.; Amaral, M.D.; Penque, D.. "Unusually common cystic fibrosis mutation in Portugal encodes a misprocessed protein". Biochemical and Biophysical Research Communications 311 3 (2003): 665-671. http://www.scopus.com/inward/record.url?eid=2-s2.0-0242577536&partnerID=MN8TOARS.
    10.1016/j.bbrc.2003.10.048
  179. Ramalho, A.S.; Beck, S.; Penque, D.; Gonska, T.; Seydewitz, H.H.; Mall, M.; Amaral, M.D.; et al. "Transcript analysis of the cystic fibrosis splicing mutation 1525-1G>A shows use of multiple alternative splicing sites and suggests a putative role of exonic splicing enhancers.". Journal of medical genetics 40 7 (2003): http://www.scopus.com/inward/record.url?eid=2-s2.0-0043209306&partnerID=MN8TOARS.
    10.1136/jmg.40.7.e88
  180. Doucet, L.; Mendes, F.; Montier, T.; Delépine, P.; Penque, D.; Férec, C.; Amaral, M.D.; et al. "Applicability of different antibodies for the immunohistochemical localization of CFTR in respiratory and intestinal tissues of human and murine origin". Journal of Histochemistry and Cytochemistry 51 9 (2003): 1191-1199. http://www.scopus.com/inward/record.url?eid=2-s2.0-0041819843&partnerID=MN8TOARS.
  181. Ramalho AS; Beck S; Meyer M; Penque D; Cutting GR; Amaral MD; Ramalho, A.S.; et al. "Five percent of normal cystic fibrosis transmembrane conductance regulator mRNA ameliorates the severity of pulmonary disease in cystic fibrosis.". American Journal of Respiratory Cell and Molecular Biology 27 5 (2002): 619-627. http://europepmc.org/abstract/med/12397022.
    10.1165/rcmb.2001-0004OC
  182. Farinha CM; Nogueira P; Mendes F; Penque D; Amaral MD; Farinha, C.M.; Nogueira, P.; et al. "The human DnaJ homologue (Hdj)-1/heat-shock protein (Hsp) 40 co-chaperone is required for the in vivo stabilization of the cystic fibrosis transmembrane conductance regulator by Hsp70.". Biochemical Journal 366 3 (2002): 797-806. http://europepmc.org/abstract/med/12069690.
    10.1042/BJ20011717
  183. Amaral MD; Pacheco P; Beck S; Farinha CM; Penque D; Nogueira P; Barreto C; et al. "Cystic fibrosis patients with the 3272-26A>G splicing mutation have milder disease than F508del homozygotes: a large European study.". Journal of Medical Genetics 38 11 (2001): 777-782. http://europepmc.org/abstract/med/11732487.
    10.1136/jmg.38.11.777
  184. Amaral, M.D.; Chen, L.; Chattopadhyay, D.; Smith, C.D.; Meehan, E.J.. "Crystallization and preliminary x-ray diffraction analysis of protein l-isoaspartyl o-methyltransferase from wheat germ". Acta Crystallographica Section D: Biological Crystallography 57 2 (2001): 304-305. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035121733&partnerID=MN8TOARS.
    10.1107/S0907444900017030
  185. Penque, D.; Mendes, F.; Beck, S.; Farinha, C.; Pacheco, P.; Nogueira, P.; Lavinha, J.; et al. "Cystic fibrosis F508del patients have apically localized CFTR in a reduced number of airway cells". Laboratory Investigation 80 6 (2000): 857-868. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033918281&partnerID=MN8TOARS.
    10.1038/labinvest.3780090
  186. Beck, S.; Penque, D.; Garcia, S.; Gomes, A.; Farinha, C.; Mata, L.; Gulbenkian, S.; et al. "Cystic fibrosis patients with the 3272-26A¿G mutation have mild disease, leaky alternative mRNA splicing, and CFTR protein at the cell membrane". Human Mutation 14 2 (1999): 133-144. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032796106&partnerID=MN8TOARS.
    10.1002/(SICI)1098-1004(1999)14:2<133::AID-HUMU5>3.0.CO;2-T
  187. Duarte, Â.; Amaral, M.; Barreto, C.; Pacheco, P.; Lavinha, J.. "Complex cystic fibrosis allele R334W-R1158X results in reduced levels of correctly processed mRNA in a pancreatic sufficient patient". Human Mutation 8 2 (1996): 134-139. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029745220&partnerID=MN8TOARS.
    10.1002/(SICI)1098-1004(1996)8:2<134::AID-HUMU5>3.0.CO;2-B
  188. Amaral, M.D.; Galego, L.; Rodrigues-Pousada, C.. "Heat-shock-induced protein synthesis is responsible for the switch-off of hsp70 transcription in Tetrahymena". BBA - Gene Structure and Expression 1174 2 (1993): 133-142. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027171174&partnerID=MN8TOARS.
    10.1016/0167-4781(93)90107-O
  189. Amaral MD; Galego L; Rodrigues-Pousada C. "Stress response of Tetrahymena pyriformis to arsenite and heat shock: differences and similarities.". (1988): http://europepmc.org/abstract/med/3126063.
    10.1111/j.1432-1033.1988.tb13812.x
  190. Bouvet OM; Grimont PA. "Diversity of the phosphoenolpyruvate/glucose phosphotransferase system in the Enterobacteriaceae.". Annales de l'Institut Pasteur. Microbiology (1987): https://doi.org/10.1016/0769-2609(87)90048-2.
    10.1016/0769-2609(87)90048-2
Thesis / Dissertation
  1. Gomes, André Miguel Carapinha. "Epithelial differentiation: from organoids to cells in cystic fibrosis patients". Master, 2019. http://hdl.handle.net/10451/40624.
  2. Centeio, Raquel Martins. "Diagnosis, Prognosis and Personalized Treatment of Cystic Fibrosis". Master, 2018. http://hdl.handle.net/10451/36482.
  3. Felício, Verónica Manuela Rôxo. "Characterization of mRNA dysfunctional mechanisms associated with the genetic disease cystic fibrosis". PhD, 2018. http://hdl.handle.net/10451/38258.
  4. Saúde, Filipe Carapinha Caldeira Batista. "Role of PKD1 and PKD2 on ion currents in renal epithelial cells". Master, 2018. http://hdl.handle.net/10451/36504.
  5. Awatade, Nikhil T, 1988-. "Using a systems approach to identify the mechanism of action of correctors". PhD, 2018. http://hdl.handle.net/10451/34859.
  6. Lérias, Joana Ramos Rapaz, 1988-. "Anoctamin 1-A member of a novel family of ion channels with extended functions and significance in disease". PhD, 2018. http://hdl.handle.net/10451/35037.
  7. Massinga, Arsénia Joana. "Study and characterization of CFTR mutations in vitro and in native tissues from non CF patients with chronic airways diseases". Master, 2017. http://hdl.handle.net/10451/30378.
  8. Petinga, Íris Lameiro. "Solute carrier transporters (SLCs) as possible drug targets for cystic fibrosis". Master, 2017. http://hdl.handle.net/10451/31226.
  9. Martins, Pedro de Almeida. "Previsão da localização subcelular de proteínas humanas com base em aprendizagem automática". Master, 2017. http://hdl.handle.net/10451/27610.
  10. Ramalho, Sofia Santos. "Characterization of orphan CFTR mutations". Master, 2017. http://hdl.handle.net/10451/31920.
  11. Fonseca, Ana Fragoso Amado Faria. "Importance of anoctamins for calcium signalling in different cellular localizations". Master, 2016. http://hdl.handle.net/10451/27296.
  12. Simões, Filipa Bica. "Role of CFTR and TMEM16 for regulated cell death". Master, 2016. http://hdl.handle.net/10451/25214.
  13. Pinto, Madalena do Carmo Fragoso. "Anoctamins - novel members of ion channels family with extended functions and significance in disease". Master, 2015. http://hdl.handle.net/10451/22437.
  14. Uliyakina, Inna, 1985-. "Analysis of CFTR mutants in epithelial cells/tissues and testing of CFTR-repairing therapies". PhD, 2015. http://hdl.handle.net/10451/20232.
  15. Ciaccioli, Gianmario. "ASYN and tau interaction : new drug target for neurodegenerative diseases". PhD, 2014. http://hdl.handle.net/10451/15500.
  16. Neto, Mário João Faustino, 1982-. "Regulating proteostasis by a chemical genetics approach". PhD, 2013. http://hdl.handle.net/10451/7991.
  17. Afonso, Sara Cerqueira. "Anoctamins: a novel family of ion channels with extended functions and significance in disease". Master, 2013. http://hdl.handle.net/10451/16079.
  18. Amorim, João Pedro Pacheco Conde de. "Characterization of CFTR nonsense mutations using novel CFTR minigenes". Master, 2013. http://hdl.handle.net/10451/10362.
  19. Faria, Diana Delgado, 1984-. "Functional characterization of proteins interacting or modulating CFTR and ENaC traffic and activity". PhD, 2012. http://hdl.handle.net/10451/7714.
  20. Sousa, Marisa Isabel Lopes de, 1983-. "Correction of the ion transport defect in Cystic Fibrosis by small molecules". PhD, 2012. http://hdl.handle.net/10451/7181.
  21. Palma, Marta Augusta Moreira Fortio da, 1972-. "Epithelial cells from lung: production, cultivation and characterization: usage for validation of compounds efficacy in rescuing mutant CFTR". Master, 2012. http://hdl.handle.net/10451/8403.
  22. Martins, Joana Raquel Delgado, 1984-. "Calcium-activated chloride in cystic fibrosis". PhD, 2011. http://hdl.handle.net/10451/3533.
  23. Peixeiro, Isabel Cristina Ramos, 1982-. "The role of mRNA translation on nonsense-mediated decay inhibition of transcripts carrying a short open reading frame". PhD, 2011. http://hdl.handle.net/10451/6198.
  24. Silva, Maria Catarina Telo Baptista Lima da, 1982-. "Genetic screens in C. elegans for new modulators of protein homeostasis, with relevance for conformational diseases". PhD, 2011. http://hdl.handle.net/10451/5343.
  25. Moraes, Bruno José Rother Rocha de. "Study of CFTR delivery and stabilization at the plasma membrane". Master, 2010. http://hdl.handle.net/10451/5661.
  26. Braz, Carla Susana Rodrigues, 1971-. "Production of a genomic CFTR construct inserted into a human artificial chromosome (HAC) and characterization of its expression". PhD, 2010. http://hdl.handle.net/10451/2248.
  27. Paula, Ana Carina Simões Mendes da. "Folding, processing and function of human-murine CFTR chimeras:implications for the structure of CFTR". PhD, 2010. http://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000579029.
  28. Almaça, Joana Collares Pereira, 1984-. "Functional characterization of lon channels with a crucial role in cystic fibrosis : regulation of epithelial Na+chanel (ENaC) and Ca2+-activated Cl- channels (CaCCs) in epithelial cells". PhD, 2010. http://hdl.handle.net/10451/2591.
  29. Pereira, Francisco José Cabral, 1978-. "Study of the nonsense-mediated decay (NMD) mechanism in the human \03B1-globin mRNA". PhD, 2009. http://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000556096.
  30. Schmidt, André, 1977-. "Restoration of cystic fibrosis transmembrane conductance regulator function to CF mutants by enhancing protein processing and channel activity". PhD, 2008. http://hdl.handle.net/10451/1546.
  31. Pissarra, Luísa Maria da Silva, 1977-. "Study of CFTR mutants and their processing properties: structural implications". PhD, 2008. http://hdl.handle.net/10451/1532.
  32. Moniz, Sónia Cristina da Rocha Pereira, 1973-. "Functional characterisation of the novel WNK2 protein kinase". PhD, 2007. http://hdl.handle.net/10451/1673.
  33. Carapau, Daniel José Martins, 1978-. "Transcriptomic analysis of the dendritic cell regulation by malaria: production of fever mediators in the absence of maturation". PhD, 2007. http://hdl.handle.net/10451/1526.

Other

Other output
  1. Full Rescue of F508del-CFTR Processing and Function by CFTR Modulators can be Achieved by Removal of Two Unique Regulatory Regions. 2018. Uliyakina I; Da Paula AC; Afonso S; Lobo MJ; Felício V; Botelho HM; Farinha CM; Amaral MD. http://europepmc.org/abstract/PPR/PPR12918.
    10.1101/320630
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2022/01/01 - 2026/12/31 Role of CFTR in Epithelial Differentiation and EMT by Functional Genomics
Supervisor of Cláudia Alexandra da Silva Rodrigues
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2019/04/01 - 2023/03/31 Personalized Therapies For All: Restoring Airway Function in CF using Alternative Chloride Channels (ACC)
Supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2016/01/01 - 2021/10/31 Role of CFTR in Epithelial to Mesenchymal Transition (EMT) by Functional Genomics
Supervisor
 Biologia (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2016/01/01 - 2021/10/31 Personalised Therapies for All: Targeting alternative chloride channels in Cystic Fibrosis
Co-supervisor
 Biologia (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2015 - 2020 Role of CFTR in Epithelial Differentiation and Wound Healing by Functional Genomics
Supervisor
 Biologia (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2014 - 2018 Using a Systems Approach to Identify the Mechanism of Action of Correctors
Supervisor
 Biologia (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2014 - 2018 Anoctamins - A Novel Family of Ion Channels With Extended Functions and Significance in Disease
Co-supervisor
 Biologia (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2013 - 2018 Characterization of RNA dysfunctional mechanisms associated with the genetic disease Cystic Fibrosis
Supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2011 - 2015 Analysis of CFTR Mutants in Epithelial Cells/Tissues and Testing of CFTR-Repairing Therapies
Supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2007 - 2013 Regulating Proteostasis by a Chemical Genetics Approach
Co-supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2009 - 2012 Functional Characterization of Proteins Interacting or Modulating CFTR and ENaC Traffic and Activity
Co-supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2008 - 2012 Correction of the Ion Transport Defect in Cystic Fibrosis by Small Molecules
Supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2007 - 2012 Genetic Screens in C. elegans for New Modulators of Protein Homeostasis, with Relevance for Conformational Diseases
Co-supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2007 - 2011 Calcium-activated chloride channels in Cystic Fibrosis
Co-supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2007 - 2011 Functional regulation of ion channels with crucial role in Cystic Fibrosis
Supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2005 - 2010 Folding, Processing and Function of Human-Murine CFTR Chimeras: Implications for the Structure of CFTR
Supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2005 - 2010 Production of a Genomic Construct Inserted into a Human Artificial Chromosome (HAC) and Characterization of its Expression
Supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2005 - 2008 Restoration of Cystic Fibrosis Transmembrane Conductance Regulator Function to CF Mutants by Enhancing Protein Processing and Channel Activity
Supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2003 - 2008 Study of CFTR Mutants and their Processing Properties: Structural Implications
Supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2001 - 2005 Characterization of Genomic Sequences of the CFTR Gene and Analysis of the Respective Transcripts
Supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2000 - 2005 Protein Profiles of Cystic Fibrosis Nasal Cells. Characterization of Mutant and Revertant Forms of the CFTR Protein
Supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
2000 - 2005 Identification of Modulators of Poly-Glutamine Folding and Aggregation in C elegans
Co-supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
1998 - 2003 Processing and Intracellular Trafficking of Wild-Type and Mutant CFTR
Supervisor
 Bioquímica (PhD)
Universidade de Lisboa Faculdade de Ciências, Portugal
Distinctions

Award

2021 Prémio Científico Universidade de Lisboa/ Caixa Geral de depósitos
2019 Thomé Villar / Boehringer Ingelheim 2019 Award
Sociedade Portuguesa de Pneumologia, Portugal
2019 John Riordan & Paul Quinton Award for CF Research
Bob Emmelkamp Association of parents and friends of people with Cystic Fibrosis, United States
2016 Award for the 2nd most productive scientist in the scientific area of Biology, Bioengineering, Biochemistry & Biotechnology
Reitoria da Universidade de Lisboa , Portugal
2013 Pfizer Award for Basic Biomedical Research
Sociedade das Ciências Médicas de Lisboa , Portugal
2010 Annual Award of European Cystic Fibrosis Society

Title

2017 Editor of Scientific Reports
Nature, United Kingdom
2014 EMBO member
EMBO- European Molecular Biology Organization, Germany
2014 Membro da Academia das Ciências de Lisboa
Academia das Ciências de Lisboa, Portugal
2012 Member of the Board of the European Cystic Fibrosis Society
European Cystic Fibrosis Society, Denmark
2010 Member of the Research Advisory Board of Mukoviszidose Institut-the Cystic Fibrosis Patients Association (Germany)
Mukoviszidose e.V. - - Bundesverband Cystische Fibrose (CF), Germany
2008 Associate Editor of Journal of Cystic Fibrosis
Elsevier BV, Netherlands
2006 Member of Research Advisory Board of the Cystic Fibrosis Trust
Cystic Fibrosis Trust, United Kingdom