Sara Francisca Ramalhosa Guerreiro (1619-7A68-CDF3)

Curriculum Vitae

Sara Francisca Ramalhosa Guerreiro

Sara Francisca Ramalhosa Guerreiro is a PhD student in Health Sciences at ICVS, Medical School, University of Minho. She obtained her BSc in Biochemistry at Sciences School, University of Minho in 2016 and her MSc in Health Sciences in the Medical School, University of Minho in 2018. She started her career working on clarifying the role Tau protein in several conditions, such as chronic pain, Alzheimer's disease and stress-induced pathology. To accomplish that, she worked with several mice models lacking (TauKO) and overexpressing (P301L) Tau protein. Next, she focused in the study of movement disorders, more specifically, Machado Joseph disease (MJD), and its possible therapeutic targets through the testing of several therapeutic strategies (NLX-112, citalopram, intermittent fasting) in mice mimicking the disease. Currently, she is focused in comprehending the molecular pathways underlying neurodevelopmental disorders, specifically Down syndrome using mice and human induced pluripotent stem cells (hiPSC). Overall, she obtained training in working with mice, such as motor, anxiogenic, cognitive, depressive and drug-preference animal behavior, surgeries, blood collection, intraperitoneal injections and euthanizing. Besides, she also has experience in tissue processing and molecular cellular techniques, such as vibratome and criostate brain and spinal cord slicing, subcellular nuclear fractionation, western blotting, DNA and mRNA extraction, PCR and RT-qPCR analysis, immunofluorescence and immunohistochemistry. Additionally, she has experience in manual neuronal reconstruction using the Golgi-cox technique and, to complement, in 2017, she received training in the University of Osnabrueck, Germany, in confocal microscopy and semi-automated neuronal reconstruction as well as in working with cellular primary cultures. In the future, she wants to better understand the mechanisms underlying the development and progression of genetic disorders since it allows the selection of more adequate therapeutic targets and, consequently, the development of better compounds.

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Identification

Personal identification

Full name: Sara Francisca Ramalhosa Guerreiro

Citation names

Guerreiro, Sara R.
Guerreiro, S.

Author identifiers

Ciência ID: 1619-7A68-CDF3
ORCID iD: 0000-0001-7521-5119

Email addresses

id10770@alunos.uminho.pt (Professional)

Telephones

Mobile phone

253604835 (Professional)

Addresses

Campus de Gualtar, 4710-057, Braga, Braga, Portugal (Professional)

Knowledge fields

Medical and Health Sciences
Medical and Health Sciences - Medical Biotechnology
Medical and Health Sciences - Health Sciences
Medical and Health Sciences - Basic Medicine - Neurosciences
Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions
Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism

Languages

Language	Speaking	Reading	Writing	Listening
Portuguese (Mother tongue)
English	Upper intermediate (B2)	Advanced (C1)	Upper intermediate (B2)	Advanced (C1)
Spanish; Castilian	Upper intermediate (B2)	Proficiency (C2)	Upper intermediate (B2)	Proficiency (C2)

Education

	Degree	Classification
2023/01 - 2027 Ongoing	Ciências da Saúde (Doutoramento) Universidade do Minho Escola de Medicina, Portugal
2023/03/06 - 2023/03/31 Concluded	Finding solutions in a dish: advanced human in vitro modeling approaches for fundamental and translational research (Outros) Universidade do Minho, Portugal
2023/01/30 - 2023/02/17 Concluded	Bioinformatics in Health Sciences (Outros) Universidade do Minho, Portugal
2021/07/25 - 2021/09/15 Concluded	Online course: "Writing in the Sciences" (Outros) Stanford University, United States	95.84%
2016/09 - 2018/06 Concluded	Health Sciences (Mestrado) Universidade do Minho Escola de Medicina, Portugal "Clarifying the role of Tau protein in the mechanisms of brain damage induced by peripheral neuropathy" (THESIS/DISSERTATION)	17
2017/04/26 - 2017/05/12 Concluded	Advanced course: "Hands On: Rodent brain (1st Edition)" (Outros) Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2017/01/30 - 2017/02/10 Concluded	Advanced course: Laboratory Animal Science (12th Edition) Based on FELASA category C recommendations (Outros) Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2017/01/09 - 2017/01/27 Concluded	Advanced course: Biostatistics (7th Edition) (Outros) Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2016/10/24 - 2016/11/10 Concluded	Advanced course: Fundamentals of Genetics, Development and Neoplasia (8th Edition) (Outros) Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2016/10/03 - 2016/10/24 Concluded	Advanced course: Fundamentals Immunology and Infection (8th Edition) (Outros) Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2016/09/12 - 2016/09/28 Concluded	Advanced course: Fundamentals in Neuroscience (9th Edition) (Outros) Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2013/09 - 2016/07 Concluded	Bioquímica (Licenciatura) Universidade do Minho Escola de Ciências, Portugal "The interplay between peripheral neuropathy and Tau protein in neuronal and synaptic damage in the brain" (THESIS/DISSERTATION)	13

Affiliation

Science

	Category Host institution	Employer
2020/03/01 - 2022/12	Research Assistant (Research)	Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
	Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2018/07 - 2019/05	Research Assistant (Research)	Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
	Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
2016/02 - 2016/08	Research Trainee (Research)	Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal
	Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal

Others

	Category Host institution	Employer
2023/01 - Current	PhD Student	Universidade do Minho Escola de Medicina, Portugal
	Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal

Projects

Grant

	Designation	Funders
2021/09 - Current	Intermittent fasting as a novel therapeutical strategy for Spinocerebellar Ataxias - Research Technician Fellow
2020/12 - Current	Serotonergic signalling activation as a therapeutic approach for neurodegenerative diseases - Research Technician Fellow
2020/03/01 - Current	Determining the Efficacy of NLX-112 in a Mouse Experimental Model of Cerebellar Ataxia Research Fellow Laboratório Associado ICVS 3B's, Portugal	US Department of Defense Ongoing
2023/01/01 - 2027/01/01	Development of over-inhibition of cortical circuits in Down Syndrome NEURON/0001/2021 PhD Student Fellow Laboratório Associado ICVS 3B's, Portugal	Associação para a Inovação e Desenvolvimento da FCT Ongoing

Other

	Designation	Funders
2017/06 - Current	Tau as converging protein in the chronic stress and Alzheimer's disease synaptic pathologies missing Other Laboratório Associado ICVS 3B's, Portugal Universität Osnabrück, Germany	Ongoing
2016/02 - Current	Clarifying the role of Tau protein biology and pathology in the mechanisms of brain damage induced by peripheral neuropathy - Other Universidade do Minho Escola de Medicina, Portugal	Ongoing
2018 - 2018	Chronic stress triggers divergent dendritic alterations in immature neurons of the adult hippocampus, depending on their ultimate terminal fields Other

Outputs

Publications

Conference abstract	Guerreiro, Sara; Sotiropoulos, Ioannis; Leite-Almeida, Hugo. "A novel role for Tau protein in the mechanisms of brain damage caused by peripheral neuropathy". Paper presented in ECNP Workshop for Junior Scientists,, Nice, 2018. Published • 10.1016/j.euroneuro.2017.12.084
Conference poster	Guerreiro, S. ; Monteiro-Fernandes, Daniela; Duarte-Silva, S; Maciel, Patrícia. "Characterization of the movement disorder phenotype of a mouse model of Down Syndrome". Paper presented in 5th T21RS International Conference, Rome (Italy), 2024. Guerreiro, S. ; Monteiro-Fernandes, Daniela; Duarte-Silva, S; Maciel, Patrícia. "Characterization of the movement disorder phenotype of a mouse model of Down Syndrome". Paper presented in Midterm symposium of the Joint Transnational Call JTC2021 “Neurodevelopmental Disorder”, 2024. Correia, Joana Sofia; Monteiro-Fernandes, Daniela; Guerreiro, Sara R.; Ferreira-Lomba, Bruna; Cunha-Garcia, Daniela ; Gomes, Patrícia; Duarte-Silva, S; Maciel, Patrícia. Corresponding author: Maciel, Patrícia. "Assessing the HPA-axis mediated stress responses in a mouse model of SCA3". Paper presented in 11th World Congress of Neuroscience IBRO 2023, 2023. Correia, Joana Sofia; Monteiro-Fernandes, Daniela; Guerreiro, Sara R.; Ferreira-Lomba, Bruna; Cunha-Garcia, Daniela ; Gomes, Patrícia; Duarte-Silva, S; Maciel, Patrícia. Corresponding author: Maciel, Patrícia. "Understanding the relevance of the cerebellar cognitive affective syndrome in SCA3 mice". Paper presented in International Congress for Ataxia Research (ICAR) 2022, 2022. Ferreira-Lomba, Bruna; Guerreiro, Sara R.; Duarte-Silva, S; Cunha-Garcia, Daniela ; Teixeira-Castro, Andreia; Vilasboas-Campos, Daniela; Vidinha-Mira, André; et al. "Motor coordination and balance ameliorated after treatment of the CMVMJD135 mouse model of Spinocerebellar Ataxia Type 3 with befiradol". Paper presented in International Congress for Ataxia Research (ICAR) 2022, 2022. Guerreiro, Sara; Ferreira-Lomba, Bruna; Cunha-Garcia, Daniela ; Duarte-Silva, S; Pereira-Sousa, Joana; Vilasboas-Campos, Daniela; Vidinha-Mira, André; et al. "Preclinical study of a 5-HT1A receptor specific agonist in the CMVMJD135 mouse model of Machado-Joseph disease". Paper presented in International Society for Neurochemistry-Asian Pacific Society of Neurochemistry (ISN-APSN) meeting 2022, Honolulu, USA, 2022. Correia, Joana Sofia; Monteiro-Fernandes, Daniela; Guerreiro, Sara R.; Ferreira-Lomba, Bruna; Cunha-Garcia, Daniela ; Gomes, Patrícia; Duarte-Silva, S; Maciel, Patrícia. Corresponding author: Maciel, Patrícia. "The study of depression and cognitive comorbidities in a mouse model of spinocerebellar ataxia type 3". Paper presented in International Society of Neurochemistry (ISN) and Asian Pacific Society of Neurochemistry (APSN) 2022 Meeting, 2022. Ferreira-Lomba, Bruna; Guerreiro, S. ; Cunha-Garcia, Daniela ; Duarte-Silva, S; Pereira-Sousa, Joana; Vilasboas-Campos, Daniela; Vidinha-Mira, André; et al. "Pre- and post-symptomatic treatment with NLX-112 improved the balance and motor coordination of the CMVMJD135 mouse model of Machado-Joseph Disease". Paper presented in Federation of European Neuroscience Societies (FENS) Forum 2022, Paris, France, 2022. Correia, Joana Sofia; Monteiro-Fernandes, Daniela; Guerreiro, Sara R.; Ferreira-Lomba, Bruna; Cunha-Garcia, Daniela ; Gomes, Patrícia; Duarte-Silva, S; Maciel, Patrícia. Corresponding author: Maciel, Patrícia. "Stress resilience in SCA3: study of depression and cognitive comorbidities in a mouse model". Paper presented in Federation of European Neuroscience Societies (FENS) Forum 2022, 2022. Guerreiro, Sara; Ferreira-Lomba, Bruna; Duarte-Silva, S; Cunha-Garcia, Daniela ; Vidinha-Mira, André; Vilasboas-Campos, Daniela; Teixeira-Castro, Andreia; et al. Corresponding author: Maciel, Patrícia. "NLX-112 improved the motor coordination and balance in a mouse experimental model of Machado-Joseph Disease". Paper presented in XVII Meeting of the Portuguese Society for Neuroscience (SPN), 2021. Vidinha-Mira, André; Monteiro, Daniela; Guerreiro, Sara R.; Almeida, Bruno; Duarte-Silva, S; Teixeira-Castro, Andreia. "Serotonergic Signalling Activation as a Therapeutic Approach for Neurodegenerative Diseases". Paper presented in Investigação Jovem da U.Porto (IJUP) 2021, 2021. Vidinha-Mira, André; Monteiro-Fernandes, Daniela; Guerreiro, Sara; Vieira, Carmen ; Cunha-Garcia, Daniela ; Ferreira-Lomba, Bruna; Oliveira, Stéphanie; et al. "Does activation of serotonergic treatment suppress proteotoxicity in mouse models of neurodegenerative diseases?". Paper presented in Sixteenth - Young European Scientist (YES) Meeting, 2021. Guerreiro, Sara; Guimarães, Marco; Sousa, Raquel; Dioli, Chrysoula; Marques, Carlos; Silva, Joana Margarida; Gomes, Patricia; et al. "A novel role for Tau protein in the mechanisms of brain damage caused by peripheral neuropathy". Paper presented in 2nd EuroTau Meeting, April, Lille, France, 2018. Guerreiro, Sara; Guimarães, Marco; Sousa, Raquel; Dioli, Chrysoula; Marques, Carlos; Silva, Joana Margarida; Gomes, Patricia; et al. "A novel role for Tau protein in the mechanisms of brain damage caused by peripheral neuropathy". Paper presented in ECNP Workshop for Junior Scientists, March, Nice, France, 2018. Guerreiro, Sara; Guimarães, Marco; Sousa, Raquel; Dioli, Chrysoula; Marques, Carlos; Silva, Joana Margarida; Gomes, Patricia; et al. "A novel role for Tau protein in the mood and cognitive deficits triggered by peripheral neuropathy". Paper presented in XV SPN Meeting, May, Braga, Portugal, 2017.
Journal article	Duarte-Silva, S; DA SILVA, JORGE D.; Monteiro-Fernandes, Daniela; Costa, Marta Daniela; Neves-Carvalho, Andreia; Raposo, Mafalda; Soares-Cunha, Carina; et al. "Glucocorticoid receptor-dependent therapeutic efficacy of tauroursodeoxycholic acid in preclinical models of Spinocerebellar ataxia type 3 (IF: 19.47)". The Journal of Clinical Investigation (2024): Published Duarte-Silva, S; DA SILVA, JORGE D.; Monteiro-Fernandes, Daniela; Costa, Marta Daniela; Neves-Carvalho, Andreia; Mafalda Raposo; Soares-Cunha, Carina; et al. "Glucocorticoid receptor-dependent therapeutic efficacy of tauroursodeoxycholic acid in preclinical models of Spinocerebellar ataxia type 3". The Journal of Clinical Investigation (2024): https://www.jci.org/articles/view/162246. In press Luísa Carvalho; Fábio Pedroso de Lima; Mónica Cerqueira; Ana Silva; Olívia Pontes; Sofia Oliveira-Pinto; Sara Guerreiro; et al. "In vitro and in vivo evaluation of novel chromeno[2,3-d]pyrimidinones as therapeutic agents for triple negative breast cancer". RSC Medicinal Chemistry (2024): https://doi.org/10.1039/D3MD00682D. 10.1039/D3MD00682D Sara Guerreiro; Patrícia Maciel. "Transition from Animal-Based to Human Induced Pluripotent Stem Cells (iPSCs)-Based Models of Neurodevelopmental Disorders: Opportunities and Challenges". Cells (2023): https://doi.org/10.3390/cells12040538. 10.3390/cells12040538 Luísa Carvalho; Pedroso de Lima, Fábio; Pontes, Olívia; Oliveira-Pinto, Sofia; Cerqueira, Mónica; Ana silva; Guerreiro, Sara R.; et al. "Synthesis and Biological Evaluation of Novel Chromeno[2,3-d]Pyrimidinones as Agents for Triple Negative Breast Cancer Treatment". RSC Medicinal Chemistry (2023): Under revision Guerreiro, Sara R.; Guimarães, Marco R.; Silva, Joana M.; Dioli, Chrysoula; Vamvaka-Iakovou, Anastasia; Sousa, Raquel; Gomes, Patrícia; et al. "Chronic pain causes Tau-mediated hippocampal pathology and memory deficits". Molecular Psychiatry (2022): http://dx.doi.org/10.1038/s41380-022-01707-3. Published • 10.1038/s41380-022-01707-3 Dioli, Chrysoula; Patrício, Patrícia; Sousa, Nuno; Kokras, Nikolaos; Dalla, Christina; Guerreiro, Sara; Santos-Silva, Miguel A.; et al. "Chronic stress triggers divergent dendritic alterations in immature neurons of the adult hippocampus, depending on their ultimate terminal fields". Translational Psychiatry 9 1 (2019): http://dx.doi.org/10.1038/s41398-019-0477-7. Published

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2024/03/16	Characterization of the movement disorder phenotype of a mouse model of Down Syndrome	Selected Oral presentation at the SPDmov 2024 Sociedade Portuguesa das Doenças do Movimento (SPDmov) (Esposende, Portugal)
2019/03/29	A novel role for Tau protein in the mechanisms of brain damage caused by peripheral neuropathy	Selected Oral presentation at the 14th International Conference of Alzheimer's and Parkinson diseases (AD/PD) 2019, March 2019, Lisbon, Portugal (Lisbon, Portugal)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2024/05 - 2024/07	Characterization of the hydrocephaly in a mouse model of Down Syndrome Co-supervisor	Universidade do Minho, Portugal

Event organisation

	Event name Type of event (Role)	Institution / Organization
2023/08/03 - 2023/08/07	2023 ISN Advanced School (2023/08/03 - 2023/08/07) Workshop (Co-organisor)	International Society for Neurochemistry, Switzerland
2021/01 - 2021/02/28	Rare Diseases Day 2021 (2021/02/28)	Laboratório Associado ICVS 3B's, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2023/08/08 - 2023/08/11	ISN-ESN Meeting, Porto Conference	ISN-ESN Meeting, Porto International Society for Neurochemistry, Switzerland
2021/09/16 - 2021/09/16	Seminar: "The Pathway to Improved Translation with Targeted In Vivo Modelling" Seminar	Charles River Analytics Inc, United States
2021/05/25 - 2021/05/26	1st European Society for Neurochemistry Virtual Conference Conference	Future Perspectives for European Neurochemistry - a young scientists conference
2021/05/06 - 2021/05/06	Seminar: "Modelling the human brain using induced pluripotent stem cells (iPSCs)" Speaker: Anjana Nityanandam, Ph.D (Director of Operations - Stem Cell Lab, St. Jude Children's Hospital) Seminar

Committee member

	Activity description Role	Institution / Organization
2024/10 - 2025/10	Member of the Communication workgroup of the T21RS Research Society Member	T21 Research Society, United States

Scientific expedition

	Activity description	Institution / Organization
2022/07/08 - 2022/07/08	Outreach activity in Caminha - Presentation about SCA3 and Neurosciences in general to students (7-14 years-old)
2020/02 - 2020/02	Outreach activity - Presentation about what a scientist do, employment options and how to successfully achieve a scientific career, to 16-18 years-old students.

Distinctions

Award

2022	Travel Fellowship for the International Society of Neurochemistry (ISN) - Asian Pacific Society of Neurochemistry (APSN) 2022 Meeting, August 28 - September 01, 2022, Honolulu, USA
2018	Travel Fellowship at the European College of Neuropsychopharmacology (ECNP) workshop for Junior Scientists, March 2018, Nice, France European College of Neuropsychopharmacology, Netherlands