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Alejandro Garrido-Maestu. Completed the Doutoramento in Methodologies and applications in life sciences in 2013 by Universidade de Vigo, Licenciatura in Biology in 2006 by Universidade de Vigo and Master Universitario in Methodologies and applications in life sciences in 2010 by Universidade de Vigo. Is Researcher in International Iberian Nanotechnology Laboratory. Published 42 articles in journals. Has 3 section(s) of books and 1 book(s). Co-supervised 2 PhD thesis(es). Has received 2 awards and/or honors. Participates and/or participated as Integration into Research Grant Fellow in 5 project(s), Post-doc in 1 project(s), Post-doc Fellow in 3 project(s), Principal investigator in 4 project(s), Research Technician Fellow in 6 project(s) and Researcher in 1 project(s). Works in the area(s) of Natural sciences with emphasis on Biological Sciences with emphasis on Microbiology and Natural sciences with emphasis on Biological Sciences with emphasis on Molecular Biology. In his curriculum Ciência Vitae the most frequent terms in the context of scientific, technological and artistic-cultural output are: Bacteriophage vB_SenS_PVP-SE2; qPCR; Phage amplification; Salmonella enteritidis; Chicken; Science & Technology; Caenorhabditis elegans ; Salmonella spp.; Listeria monocytogenes; E. coli O157; PCR; Electrophoresis; LAMP; RPA; MinION sequencing; Vibrio spp.; Staphylococcus aureus; STEC; Campylobacter spp.; Microbiology; Molecular Biology; Antibiotic Resistance; CRISPR-Cas; naked-eye detection; lateral flow; foodborne pathogens; DNA extraction; DNA purification; nanomaterials; COF; Antimicrobial resistance; Volatile Organic Compounds; Sensors; Hospital-acquired infection; .
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Identification

Personal identification

Full name
Alejandro Garrido-Maestu

Citation names

  • Garrido-Maestu, Alejandro
  • Garrido, Alejandro

Author identifiers

Ciência ID
1816-2D13-1AA8
ORCID iD
0000-0003-4587-8418
Google Scholar ID
https://scholar.google.es/citations?user=unZ9J_IAAAAJ&hl=es
Researcher Id
H-1577-2016
Scopus Author Id
55815800800

Email addresses

  • a.garridomaestu@gmail.com (Personal)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Microbiology
  • Natural sciences - Biological Sciences - Molecular Biology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Spanish; Castilian Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Portuguese Beginner (A1) Beginner (A1) Beginner (A1) Beginner (A1)
Education
Degree Classification
2013
Concluded
 Methodologies and applications in life sciences (Doutoramento)
Universidade de Vigo, Spain
"Application of real-time PCR for the detection of pathogens in the food industry" (THESIS/DISSERTATION)
 Cum laude
2010
Concluded
 Methodologies and applications in life sciences (Master Universitario)
Universidade de Vigo, Spain
"Application of real-time PCR for the detection of pathogenic Vibrio parahaemolyticus (tdh+ and trh+) and comparison with the method described by ISO 21872-1:2007" (THESIS/DISSERTATION)
 9.2
2006
Concluded
 Biology (Licenciatura)
Universidade de Vigo, Spain
"-" (THESIS/DISSERTATION)
 5.2
Affiliation

Science

Category
Host institution 		Employer
2020/01/02 - Current Researcher (Research) International Iberian Nanotechnology Laboratory, Portugal
2018/07 - 2020/01 Researcher (Research) International Iberian Nanotechnology Laboratory, Portugal
2015/07 - 2018/06 Postdoc (Research) International Iberian Nanotechnology Laboratory, Portugal
2015/01 - 2015/06 Postdoc (Research) University of Florida Emerging Pathogens Institute, United States
2007/10 - 2014/12 Science and Technology Management ANFACO-CECOPESCA, Spain

Others

Category
Host institution 		Employer
2007/04 - 2007/06 Private company Ecofloat Control Systems S.L, Spain
Projects

Grant

Designation Funders
2020/12/31 - 2023/06/30 R&W Clean - Novas soluções para sensorização de parâmetros ambientais e biológicos para auxílio àdesmedicalização do sector agro-pecuário
070109
Researcher
International Iberian Nanotechnology Laboratory, Portugal
 European Regional Development Fund
Ongoing
2022/03/21 - 2022/03/25 Application of DNA sequencing for the detection of foodborne pathogens
144
Mobility grant
2019 - 2022 SEAFOOD-AGE
EAPA_758/2018
Integration into Research Grant Fellow
International Iberian Nanotechnology Laboratory, Portugal
 European Regional Development Fund
Ongoing
2021/01/01 - 2021/12/31 COF-enhanced qPCR
-
Principal investigator
International Iberian Nanotechnology Laboratory, Portugal
 International Iberian Nanotechnology Laboratory
Ongoing
2020/12/01 - 2021/08/31 Highly sensitive immunoassay for SARS-CoV2 detection using new amplification technology (HighSenseCoV2)
NORTE-01-02B7-FEDER-069951
Researcher
International Iberian Nanotechnology Laboratory, Portugal
 Agência Nacional de Inovação SA
Ongoing
2018/06/15 - 2021/06/14 Control of Port and Douro Wines authenticity using graphene DNA sensors project (PORTGRAPHE)
POCI-01-0145-FEDER-031069
Researcher
Fundação para a Ciência e a Tecnologia, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2020/06/01 - 2021/03/31 Coronavirus Transmission: Count and Detect (COUNTED)
POCI-01-02B7-FEDER-050226
Researcher
International Iberian Nanotechnology Laboratory, Portugal
 Agência Nacional de Inovação SA
Ongoing
2020/07/09 - 2021/01/08 CRISPR technology against the COVID-19 pandemic. The DETECTR of SARS-CoV-2
480
Principal investigator
International Iberian Nanotechnology Laboratory, Portugal
 Associação para a Inovação e Desenvolvimento da FCT
2018 - 2021 NanoBioSensor – Development of nanosensors to evaluate the microbiological quality of fruit-based products
POCI-FEDER-033925
Integration into Research Grant Fellow
International Iberian Nanotechnology Laboratory, Portugal
 Agência Nacional de Inovação SA
Ongoing
2018 - 2021 PACKTERIOPHAGE
POCI-01-0145-FEDER-032594
Integration into Research Grant Fellow
International Iberian Nanotechnology Laboratory, Portugal
 Associação para a Inovação e Desenvolvimento da FCT
2020/04/01 - 2020/12/31 Innovative Customer-Oriented Safe Solutions (ICONSS)
20431
Researcher
International Iberian Nanotechnology Laboratory, Portugal
 EIT Food iVZW
Concluded
2020/06 - 2020/08 LAMP-Light in the diagnosis of COVID-19
165_596847607
165_596847607
Principal investigator
International Iberian Nanotechnology Laboratory, Portugal
 Associação para a Inovação e Desenvolvimento da FCT
2014 - 2020 NANOEATERS: Valorization and transfer of NANOtechnologies to EArly adopTERS of the Euroregion Galicia-Norte Portugal
0181_NANOEATERS_1_EP
Post-doc Fellow
International Iberian Nanotechnology Laboratory, Portugal
 European Regional Development Fund
Ongoing
2016/04/01 - 2018/03/31 Nanotechnology based functional solutions
NORTE-01-0145-FEDER-000019
Researcher
Comissao de Coordenacao e Desenvolvimento Regional do Norte
Concluded
2015/07/01 - 2017/06/30 Multipathogen detection lab-on-a-chip based on Loop-mediated Isothermal Amplification combined with gold nanoparticles
600375
Principal investigator
International Iberian Nanotechnology Laboratory, Portugal
 European Commission
Concluded
2015 - 2017 SF4SF: Smart factory for safe foods. “Increase the productive efficiency of food pro-cessing plants and the food safety of products made by integrating faster, sensitive, efficient and sustainable emerging technologies into the plant for the detection, elimination and man-agement of microbiolog
EXP-00082964 / ITC-20151195
Post-doc Fellow
International Iberian Nanotechnology Laboratory, Portugal
 Gobierno de España Ministerio de Industria Energía y Turismo
Concluded
2015/01/05 - 2015/06/03 Development of chitosan nanoparticles targeting pathogenic E. coli in beef and dairy cattle
2014-67021-21597
Post-doc
University of Florida Emerging Pathogens Institute, United States
 National Institute of Food and Agriculture
Concluded
2014 - 2015 Fiskeri-og havbruksnæringens forskningsfond (FHF). “Development and imple-mentation of technical criteria for the differentiation of light vs. Fully salted/ desalted cod products. How to disseminate appropriate information and avoid misleading consumers
-
Integration into Research Grant Fellow
ANFACO-CECOPESCA, Spain
 Fiskeri og havbruksnæringens forskningsfinansiering
Concluded
2010 - 2013 ECOSON
10DPI14CT
Research Technician Fellow
ANFACO-CECOPESCA, Spain
 Xunta de Galicia
Concluded
2010 - 2012 Development of molecular tools for the diagnostic of bacterial and parasitic diseases in cultured sole
10MMA006E
Research Technician Fellow
ANFACO-CECOPESCA, Spain
 Xunta de Galicia
Concluded
2010 - 2012 Pharmatlantic. Knowledge Transfer Network for Prevention of Mental Diseases and Cancer in the Atlantic Area
ERDF, No. 2009-1/117
Research Technician Fellow
ANFACO-CECOPESCA, Spain
 European Regional Development Fund
Concluded
2010 - 2011 Development of active containers with natural additives obtained from agro-industrial residues
PID-600200-2009-18
Research Technician Fellow
ANFACO-CECOPESCA, Spain
 Gobierno de España
2008 - 2010 Development of an active container for the control of microorganisms that alter fish and refrigerated fishing products; and also a molecular method for the evaluation of its effectiveness
PGIDIT08TAL003CT
Research Technician Fellow
Xunta de Galicia
Concluded
2008 - 2010 Exploitation of marine sponges or viability of their culture to obtain active substances
08MMA011CT
Research Technician Fellow
ANFACO-CECOPESCA, Spain
 Xunta de Galicia
Concluded

Contract

Designation Funders
2019 - Current CULTURE
-
Integration into Research Grant Fellow
International Iberian Nanotechnology Laboratory, Portugal

Gradiant, Spain

Bahía Software, Spain
European Commission
Ongoing
2016 - 2019 Development of a system for early detection of the Zebra mussel through analysis of enrivonmental DNA
-
Post-doc Fellow
International Iberian Nanotechnology Laboratory, Portugal
 Confederación Hidrográfica del Guadalquivir
Concluded
Outputs

Publications

Book
  1. Garrido-Maestu, Alejandro. Evaluación de riesgos microbiológicos en alimentos listos para consumo. 2012.
Book chapter
  1. A. Otero; M.J. Chapela; P. Fajardo; Garrido-Maestu, Alejandro; A.G. Cabado. "Genetic tools for aquatic toxin detection". In Seafood and Freshwater Toxins, edited by Luis Botana. CRC Press, 2019.
    Published
  2. Garrido-Maestu, Alejandro; Tomás Fornés, David; Prado Rodríguez, Marta. "The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens". In Foodborne Bacterial Pathogens, edited by Arnaud Bridier. Springer New York, 2018.
    Published • doi
  3. A. Martínez; Garrido-Maestu, Alejandro; B. Ben; M.J. Chapela; V. González; J.M. Vieites; A.G. Cabado. "Marine Biotoxins". In Hb25_Springer Handbook of Marine Biotechnology, edited by Se-Kwon Kim. Springer Berlin Heidelberg, 2015.
    Published
Conference poster
  1. Garrido-Maestu, Alejandro; F. Roumani, S. Azinheiro, C. Rodrigues, M. Prado. "A DNA-based fast method for the detection of spoilage fungi in fruit preparations". Paper presented in 9th International Symposium on Recent Advances in Food Analysis (RAFA 2019), 2019.
  2. J. Carvalho, L. Diéguez, S. Yadav, S. Azinheiro, J. Barros-Velázquez, M. Prado; Garrido-Maestu, Alejandro. "Disposable Miniaturized Device for DNA Purification from Complex Samples". Paper presented in XX EuroFoodChem, 2019.
  3. Garrido-Maestu, Alejandro; S. Azinheiro, J. Carvalho, P. Fuciños, M. Prado. "Naked-Eye Detection of E. coli O157 by Recombinase Polymerase Amplification and SYBR Green I". Paper presented in IAFP’s European Symposium on Food Safety, 2019.
  4. Garrido-Maestu, Alejandro; J. Carvalho, S. Azinheiro, A. Ipatov, R. Guerreiro, M. Elumalai, L. Diéguez, J. Gaspar, J. Barro. "Miniaturization and Optimization of Devices for Different DNA Analysis Steps". Paper presented in Mission 10000, 2018.
  5. S. Azinheiro, J. Carvalho, M. Prado; Garrido-Maestu, Alejandro. "Antimicrobial Activity of Chitosan Nanoparticles in E. coli O157". Paper presented in Mission 10000, 2018.
  6. Garrido-Maestu, Alejandro; S. Azinheiro, J. Carvalho, M. Prado. "Isothermal DNA Amplification and their Advantages for Foodborne Pathogen Detection". Paper presented in Mission 10000, 2018.
  7. S. Azinheiro, J. Carvalho, M. Prado; Garrido-Maestu, Alejandro. "Sample Pre-treatment Improvement for Faster Pathogen Detection in Foodstuff". Paper presented in Mission 10000, 2018.
  8. Garrido-Maestu, Alejandro; S. Azinheiro, J. Carvalho, M. Prado. "Detection of Listeria monocytogenes by combination of Immunomagnetic separation and Real-Time RPA in smoked salmon". Paper presented in 40th International Conference on Environmental & Food Monitoring, 2018.
  9. J. Carvalho, S. Azinheiro, J. Barros-Velázquez, M. Prado; Garrido-Maestu, Alejandro. "Development of Real-Time Loop-mediated isothermal AMPlification (qLAMP) and Polymer-ase Chain Reaction (qPCR) methods for detection of Dreissena polymorpha in the Guadal-quivir river basin". Paper presented in 40th International Conference on Environmental & Food Monitoring,, 2018.
  10. Garrido-Maestu, Alejandro; S. Azinheiro, P. Fuciños, J. Carvalho, M. Prado. "Isothermal DNA amplification for Salmonella spp. detection and characterization". Paper presented in 2nd International Conference on Food Microbiology, 2017.
  11. Garrido-Maestu, Alejandro; S. Azinheiro, P. Fuciños, J. Carvalho, M. Prado. "Development and evaluation of different DNA amplification approaches based on Loop-mediated isothermal amplification and real-time PCR for the detection gluten-containing ce-reals in food samples". Paper presented in 8th International Symposium on Recent Advances in Food Analysis (RAFA 2017), 2017.
  12. S. Azinheiro, J. Carvalho, M. Prado; Garrido-Maestu, Alejandro. "Loop-mediated isothermal amplification (LAMP) for foodborne pathogen detection: evaluation of different genetic targets for Salmonella enterica serovar Enteritidis and Typhimurium characterization". Paper presented in 8th International Symposium on Recent Advances in Food Analysis (RAFA 2017), 2017.
  13. Garrido-Maestu, Alejandro; J. Carvalho, G. Puertas, S. Azinheiro, J. Gaspar, L. Diéguez, J. Barros-Velázquez, Manuel Vázque. "Miniaturized devices for sample preparation providing highly efficient DNA extraction from olive oil". Paper presented in 8th International Symposium on Recent Advances in Food Analysis (RAFA 2017), 2017.
  14. G. Puertas, J. Carvalho, J. Gaspar, L. Diéguez, S. Azinheiro, M. Vázquez, M. Prado; Garrido-Maestu, Alejandro. "Optimization of a DNA purification protocol by microscale solid phase extraction (µSPE) and microfluidics for food products on a washable and reusable device". Paper presented in ExTech 2017, 2017.
  15. M. Cerqueira, V. D. F. Martins, J. M. R. Curto, P. Fuciños, Lorenzo Pastrana; Garrido-Maestu, Alejandro. "Active Cellulose/ Ethyl Cellulose Bi-layer Films: Development And Characterization". Paper presented in 3rd International Conference on Natural Fibers – Advanced Materials for a Greener World, 2017.
  16. Garrido-Maestu, Alejandro; M.J. Chapela, M. Ferreira, A. Ruiz Cruz, I. Martín Varela, J. Fernández. "Development and application of molecular techniques for fast diagnosis of bacterial diseases in turbot tissue". Paper presented in XIX Forum of marine resources and aquaculture of the Galician Rias, 2016.
  17. Garrido-Maestu, Alejandro; M. Ferreira, M-J. Chapela, Celia Varela, L. Arregui. "Development of a multiplex real-time PCR method for the diagnosis of three bacterial diseases in rainbow trout". Paper presented in XIX Forum of marine resources and aquaculture of the Galician Rias, 2016.
  18. Garrido-Maestu, Alejandro; P. Fuciños, E. Carbó-Argibay, Y. V. Kolen’ko, M. Prado. "Detection of Salmonella spp. by Loop-mediated isothermal AMPlification, combined with gold nanoparticles". Paper presented in XX National Conference on Food Microbiology, 2016.
  19. Z. Ma, SYR. Paik, N. Chen, S. Ko, Z. Tong, K. C. Jeong; Garrido-Maestu, Alejandro. "Engineering of Chitosan-Driven Nanoparticles to Enhance Antimicrobial Activity against Foodborne Pathogen Escherichia coli O157:H7". Paper presented in International Association for Food Protection, 2016.
  20. Z. Ma, K. C. Jeong; Garrido-Maestu, Alejandro. "Engineering of Chitosan-based Nanoparticles for pathogenic microorganisms in animals". Paper presented in Gordon Research Conferences, 2015.
  21. Garrido-Maestu, Alejandro; Z. Ma, K. C. Jeong. "In situ antimicrobial activity of chitosan nanoparticles". Paper presented in Emerging Pathogens Institute Research Day, 2015.
  22. Garrido-Maestu, Alejandro; Rodriguez-Souto, A. Pastoriza-Fontan, A. Lozano-Leon. "Action and celular effects of adherence and citotoxicity produced by European clinical and environmental strain of Vibrio parahaemolyticus". Paper presented in 49th U.S.-Japan Conference on Cholera and Other Enteric Bacterial Infections, 2015.
  23. Garrido-Maestu, Alejandro; M.J. Chapela, J. M. Vieites, A. G. Cabado. "Listeria monocytogenes boiled frozen mussels cross-contamination". Paper presented in II International Scientific Symposium on Innovation in Marine and Food Indus-try. “Sustainable Future Food”, 2014.
  24. Garrido-Maestu, Alejandro; M.J. Chapela, B. Román, E. Peñaranda, J. M. Vieites, A. G. Cabado. "Detection of pathogenic species of Vibrio genus by multiplex qPCR". Paper presented in Workshop on Food Safety of life bivalve mollusks, 2014.
  25. Garrido-Maestu, Alejandro; R. Rodriguez-Souto, A. Lozano-Leon. "Dynamics of Vibrio parahaemolyticus with virulence genes detected in mussel (Mytilus gal-loprovincialis) in Galicia harvesting areas.". Paper presented in Workshop on Food Safety of life bivalve mo, 2014.
  26. Garrido-Maestu, Alejandro; M.J. Chapela, B. Román, J. M. Vieites, A. G. Cabado. "New multiplex qPCR method for Vibrio vulnificus detection, an environmental bacterium and emerging pathogen". Paper presented in Food Analysis 2014, National Food Center, 2014.
  27. Garrido-Maestu, Alejandro; M.J. Chapela, J.M. Vieites, A.G. Cabado. "Salmonella spp. and Listeria monocytogenes Detection in Food Samples Combining a Single Step Enrichment with Multiplex Real-Time PCR". Paper presented in European Symposium on Food Safety, 2012.
  28. Garrido-Maestu, Alejandro; M.J. Chapela, P. Fajardo, M. Ferreira, M. Atanassova, J. Lago, J.M. Vieites, A. Cabado. "Development of a Real-Time PCR method for the detection of pathogenic V. parahaemolyticus in seafood and water samples". Paper presented in Vibrio 2011, 2011.
  29. Garrido-Maestu, Alejandro; Chapela M.J., Ferreira M., Atanassova M., Lago J., Fajardo P., Vieites J.M., Cabado A.G.. "Detection of pathogenic Vibrio parahaemolyticus (tdh + and trh +) in seafood by real time PCR. Comparison with ISO 21872-1:2007". Paper presented in Food Innova 2010, 2010.
  30. Chapela M.J., Ferreira M., Atanassova M., Lago J., Vieites J.M., Cabado A.G.; Garrido-Maestu, Alejandro. "A new multiplex real time PCR platform for rapid and simultaneous detection of Salmonella, Shigella and Listeria monocytogenes in seafood products". Paper presented in 40th WEFTA Annual Meeting, 2010.
  31. Garrido-Maestu, Alejandro; Chapela M.J., Lago J., Ferreira M., Atanassova M., Vieites J.M., Cabado A.G.. "Comparison of real time PCR and ISO 21872-1:2007 for the detection of pathogenic Vibrio parahaemolyticus". Paper presented in VIII Meeting of the Microbiology of Aquatic Medium, 2010.
  32. Chapela M.J., Fajardo P., Ferreira M., Atanassova M., Lago J., Vieites J.M., Cabado A.G.; Garrido-Maestu, Alejandro. "Serotyping of pathogenic microorganisms using real time PCR: Identification of toxigenic Vibrio cholerae". Paper presented in III Congress of Parga Pondal and Ramón y Cajal researchers from Galicia, 2009.
  33. B. Buján, G. Marcote, J. Lago, J. M. Vieites y A. G. Cabado; Garrido-Maestu, Alejandro. "Validation of a fast Analysis method for the quantification of microbiological parameters". Paper presented in VI Reunion of the Microbial Biotechnology and Industrial Microbiology group of the SEM, 2008.
Journal article
  1. Foteini Roumani; Sarah Azinheiro; Cristina Rodrigues; Jorge Barros-Velázquez; Alejandro Garrido-Maestu; Marta Prado. "Development of a real-time PCR assay with an internal amplification control for the detection of spoilage fungi in fruit preparations". Food Control (2022): https://doi.org/10.1016/j.foodcont.2021.108783.
    10.1016/j.foodcont.2021.108783
  2. Foteini Roumani; Saioa Gómez; Cristina Rodrigues; Jorge Barros-Velázquez; Alejandro Garrido-Maestu; Marta Prado. "Development and evaluation of a real-time fluorescence, and naked-eye colorimetric, loop-mediated isothermal amplification-based method for the rapid detection of spoilage fungi in fruit preparations". Food Control 135 (2022): 108784-108784. https://doi.org/10.1016/j.foodcont.2021.108784.
    10.1016/j.foodcont.2021.108784
  3. Sarah Azinheiro; Dipak Ghimire; Joana Carvalho; Marta Prado; Alejandro Garrido-Maestu. "Next-day detection of viable Listeria monocytogenes by multiplex reverse transcriptase real-time PCR". Food Control 133 (2022): 108593-108593. https://doi.org/10.1016/j.foodcont.2021.108593.
    10.1016/j.foodcont.2021.108593
  4. Sarah Azinheiro; Foteini Roumani; Laura Rodríguez-Lorenzo; Joana Carvalho; Marta Prado; Alejandro Garrido-Maestu. "Combination of Recombinase Polymerase Amplification with SYBR Green I for naked-eye, same-day detection of Escherichia coli O157:H7 in ground meat". Food Control 132 (2022): 108494-108494. https://doi.org/10.1016/j.foodcont.2021.108494.
    10.1016/j.foodcont.2021.108494
  5. Antonio Lozano-León; Carlos García-Omil; Rafael R. Rodríguez-Souto; Alexandre Lamas; Alejandro Garrido-Maestu. "An Evaluation of the Pathogenic Potential, and the Antimicrobial Resistance, of Salmonella Strains Isolated from Mussels". Microorganisms (2022): https://doi.org/10.3390/microorganisms10010126.
    10.3390/microorganisms10010126
  6. Sarah Azinheiro; Joana Carvalho; Pablo Fuciños; Lorenzo Pastrana; Marta Prado; Alejandro Garrido-Maestu. "Short pre-enrichment and modified matrix lysis. A comparative study towards same-day detection of Listeria monocytogenes". LWT 154 (2022): 112900-112900. https://doi.org/10.1016/j.lwt.2021.112900.
    10.1016/j.lwt.2021.112900
  7. Sarah Azinheiro; Foteini Roumani; Joana Carvalho; Marta Prado; Alejandro Garrido-Maestu. "Suitability of the MinION long read sequencer for semi-targeted detection of foodborne pathogens". Analytica Chimica Acta 1184 (2021): 339051-339051. https://doi.org/10.1016/j.aca.2021.339051.
    10.1016/j.aca.2021.339051
  8. Sarah Azinheiro; Joana Carvalho; Marta Prado; Alejandro Garrido-Maestu. "Correction: Azinheiro et al. Multiplex Detection of Salmonella spp., E. coli O157 and L. monocytogenes by qPCR Melt Curve Analysis in Spiked Infant Formula. Microorganisms 2020, 8, 1359". Microorganisms (2021): https://doi.org/10.3390/microorganisms9071482.
    10.3390/microorganisms9071482
  9. Foteini Roumani; Sarah Azinheiro; Joana Carvalho; Marta Prado; Alejandro Garrido-Maestu. "Loop-mediated isothermal amplification combined with Immunomagnetic separation and Propidium Monoazide for the specific detection of viable Listeria monocytogenes in milk products, with an Internal Amplification Control". Food Control (2021): https://doi.org/10.1016/j.foodcont.2021.107975.
    10.1016/j.foodcont.2021.107975
  10. Garrido-Maestu, Alejandro; Azinheiro, Sarah; Fuciños, Pablo; Carvalho, Joana; Prado, Marta. "Comparative study of multiplex real-time recombinase polymerase amplification and ISO 11290-1 methods for the detection of Listeria monocytogenes in dairy products". Food Microbiology 92 (2020): 103570. http://dx.doi.org/10.1016/j.fm.2020.103570.
    10.1016/j.fm.2020.103570
  11. Freitas, Filipa M.C.; Cerqueira, Miguel A.; Gonçalves, Catarina; Azinheiro, Sarah; Garrido-Maestu, Alejandro; Vicente, António A.; Pastrana, Lorenzo M.; Teixeira, José A.; Michelin, Michele. "Green synthesis of lignin nano- and micro-particles: Physicochemical characterization, bioactive properties and cytotoxicity assessment". International Journal of Biological Macromolecules 163 (2020): 1798-1809. http://dx.doi.org/10.1016/j.ijbiomac.2020.09.110.
    10.1016/j.ijbiomac.2020.09.110
  12. Garrido-Maestu, Alejandro; Azinheiro, Sarah; Roumani, Foteini; Carvalho, Joana; Prado, Marta. "Application of Short Pre-enrichment, and Double Chemistry Real-Time PCR, Combining Fluorescent Probes and an Intercalating Dye, for Same-Day Detection and Confirmation of Salmonella spp. and Escherichia coli O157 in Ground Beef and Chicken Samples". Frontiers in Microbiology 11 (2020): http://dx.doi.org/10.3389/fmicb.2020.591041.
    10.3389/fmicb.2020.591041
  13. Azinheiro, Sarah; Carvalho, Joana; Prado, Marta; Garrido-Maestu, Alejandro. "Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces". Foods 9 9 (2020): 1249. http://dx.doi.org/10.3390/foods9091249.
    Published • 10.3390/foods9091249
  14. Azinheiro, Sarah; Carvalho, Joana; Prado, Marta; Garrido-Maestu, Alejandro. "Multiplex Detection of Salmonella spp., E. coli O157 and L. monocytogenes by qPCR Melt Curve Analysis in Spiked Infant Formula". Microorganisms 8 9 (2020): 1359. http://dx.doi.org/10.3390/microorganisms8091359.
    Published • 10.3390/microorganisms8091359
  15. Azinheiro, Sarah; Kant, Krishna; Shahbazi, Mohammad-Ali; Garrido-Maestu, Alejandro; Prado, Marta; Dieguez, Lorena. "A smart microfluidic platform for rapid multiplexed detection of foodborne pathogens". Food Control 114 (2020): 107242. http://dx.doi.org/10.1016/j.foodcont.2020.107242.
    Published • 10.1016/j.foodcont.2020.107242
  16. Wu, Lei; Teixeira, Alexandra; Garrido-Maestu, Alejandro; Muinelo-Romay, Laura; Lima, Luis; Santos, Lúcio Lara; Prado, Marta; Diéguez, Lorena. "Profiling DNA mutation patterns by SERS fingerprinting for supervised cancer classification". Biosensors and Bioelectronics (2020): 112392. http://dx.doi.org/10.1016/j.bios.2020.112392.
    Accepted • 10.1016/j.bios.2020.112392
  17. Garrido-Maestu, Alejandro; Azinheiro, Sarah; Carvalho, Joana; Espiña, Begoña; Prado, Marta. "Evaluation and implementation of commercial antibodies for improved nanoparticle-based immunomagnetic separation and real-time PCR for faster detection of Listeria monocytogenes". Journal of Food Science and Technology (2020): http://dx.doi.org/10.1007/s13197-020-04450-1.
    Published • 10.1007/s13197-020-04450-1
  18. Alexandra Teixeira; Juan L. Paris; Foteini Roumani; Lorena Diéguez; Marta Prado; Begoña Espiña; Sara Abalde-Cela; et al. "Multifuntional Gold Nanoparticles for the SERS Detection of Pathogens Combined with a LAMP–in–Microdroplets Approach". Materials 13 8 (2020): 1934. https://doi.org/10.3390/ma13081934.
    10.3390/ma13081934
  19. Alejandro Garrido-Maestu; Sarah Azinheiro; Joana Carvalho; Pablo Fuciños; Marta Prado. "Optimized sample treatment, combined with real-time PCR, for same-day detection of E. coli O157 in ground beef and leafy greens". Food Control (2020): https://doi.org/10.1016/j.foodcont.2019.106790.
    10.1016/j.foodcont.2019.106790
  20. Laura Rodríguez-Lorenzo; Alejandro Garrido-Maestu; Arun K. Bhunia; Begoña Espiña; Marta Prado; Lorena Diéguez; Sara Abalde-Cela. "Gold Nanostars for the Detection of Foodborne Pathogens via Surface-Enhanced Raman Scattering Combined with Microfluidics". ACS Applied Nano Materials (2019): https://doi.org/10.1021/acsanm.9b01223.
    10.1021/acsanm.9b01223
  21. Alejandro Garrido-Maestu; Sarah Azinheiro; Joana Carvalho; Marta Prado. "Combination of Immunomagnetic Separation and Real-Time Recombinase Polymerase Amplification (IMS-qRPA) for Specific Detection of Listeria monocytogenes in Smoked Salmon Samples". Journal of Food Science (2019): https://doi.org/10.1111/1750-3841.14662.
    10.1111/1750-3841.14662
  22. Garrido-Maestu, Alejandro; Fuciños, Pablo; Azinheiro, Sarah; Carvalho, Carla; Carvalho, Joana; Prado, Marta. "Specific detection of viable Salmonella Enteritidis by phage amplification combined with qPCR (PAA-qPCR) in spiked chicken meat samples". Food Control 99 (2019): 79-83. http://dx.doi.org/10.1016/j.foodcont.2018.12.038.
    Published • 10.1016/j.foodcont.2018.12.038
  23. María-José Chapela; Martiña Ferreira; Celia Varela; Luz Arregui; Alejandro Garrido-Maestu. "Development of a multiplex real-time PCR method for early diagnosis of three bacterial diseases in fish: A real-case study in trout aquaculture". Aquaculture 496 (2018): 255-261. https://doi.org/10.1016/j.aquaculture.2018.07.003.
    10.1016/j.aquaculture.2018.07.003
  24. Garrido-Maestu, Alejandro. "Engineering of chitosan-derived nanoparticles to enhance antimicrobial activity against foodborne pathogen Escherichia coli O157:H7". Carbohydrate Polymers (2018): https://www.sciencedirect.com/science/article/pii/S0144861718307008.
    10.1016/j.carbpol.2018.06.046
  25. Joana Carvalho; Renato Negrinho; Sarah Azinheiro; Alejandro Garrido-Maestu; Jorge Barros-Velázquez; Marta Prado. "Novel approach for accurate minute DNA quantification on microvolumetric solutions". Microchemical Journal 138 (2018): 540-549. https://doi.org/10.1016/j.microc.2018.02.001.
    10.1016/j.microc.2018.02.001
  26. Garrido-Maestu, Alejandro; Alejandro Garrido-Maestu; Sarah Azinheiro; Pablo Fuciños; Joana Carvalho; Marta Prado. "Highly sensitive detection of gluten-containing cereals in food samples by real-time Loop-mediated isothermal AMPlification (qLAMP) and real-time polymerase chain reaction (qPCR)". Food Chemistry 246 (2018): 156-163. http://www.sciencedirect.com/science/article/pii/S0308814617318137.
    https://doi.org/10.1016/j.foodchem.2017.11.005
  27. Garrido-Maestu, Alejandro. "Development and evaluation of loop-mediated isothermal amplification, and Recombinase Polymerase Amplification methodologies, for the detection of Listeria monocytogenes in ready-to-eat food samples". Food Control (2018): http://www.sciencedirect.com/science/article/pii/S0956713517305285.
    https://doi.org/10.1016/j.foodcont.2017.11.006
  28. Garrido-Maestu, Alejandro. "Evaluation of Different Genetic Targets for Salmonella enterica Serovar Enteriditis and Typhimurium, Using Loop-Mediated Isothermal AMPlification for Detection in Food Samples". Frontiers in sustainable food systems (2018): https://www.frontiersin.org/articles/10.3389/fsufs.2018.00005/full.
    10.3389/fsufs.2018.00005
  29. María-José Chapela; Martiña Ferreira; Asela Ruiz-Cruz; Iris Martin-Varela; Jacobo Fernández-Casal; Alejandro Garrido-Maestu. "Application of real-time PCR for early diagnosis of diseases caused by Aeromonas salmonicida, Vibrio anguillarum, and Tenacibaculum maritimum in turbot: A field study". Journal of Applied Aquaculture (2018): 1-14. https://doi.org/10.1080/10454438.2017.1406419.
    10.1080/10454438.2017.1406419
  30. Garrido-Maestu, Alejandro. "Comprehensive in vitro and in vivo risk assessments of chitosan microparticles using human epithelial cells and Caenorhabditis elegans". Journal of Hazardous Materials 341 (2018): 248-256. http://www.sciencedirect.com/science/article/pii/S0304389417305873.
    https://doi.org/10.1016/j.jhazmat.2017.07.071
  31. Rafael R. Rodriguez-Souto; Alejandro Garrido-Maestu; Alejandro Pastoriza-Fontan; Antonio Lozano-Leon. "Investigation and characterization of Shiga toxin-producing Escherichia coli present in mussels from harvesting areas in Galician southern Rias (NW Spain)". Journal of Food Safety 37 4 (2017): e12367-e12367. https://doi.org/10.1111/jfs.12367.
    10.1111/jfs.12367
  32. Alejandro Garrido-Maestu; Sarah Azinheiro; Joana Carvalho; Sara Abalde-Cela; Enrique Carbó-Argibay; Lorena Diéguez; Marek Piotrowski; Yury V. Kolen’ko; Marta Prado. "Combination of Microfluidic Loop-Mediated Isothermal Amplification with Gold Nanoparticles for Rapid Detection of Salmonella spp. in Food Samples". Frontiers in Microbiology 8 (2017): https://doi.org/10.3389%2Ffmicb.2017.02159.
    10.3389/fmicb.2017.02159
  33. Alejandro Garrido-Maestu; Pablo Fuciños; Sarah Azinheiro; Joana Carvalho; Marta Prado. "Systematic loop-mediated isothermal amplification assays for rapid detection and characterization of Salmonella spp., Enteritidis and Typhimurium in food samples". Food Control 80 (2017): 297-306. https://doi.org/10.1016/j.foodcont.2017.05.011.
    10.1016/j.foodcont.2017.05.011
  34. Alejandro Garrido-Maestu; Antonio Lozano-León; Rafael R. Rodríguez-Souto; Ramón Vieites-Maneiro; María-José Chapela; Ana G. Cabado. "Presence of pathogenic Vibrio species in fresh mussels harvested in the southern Rias of Galicia (NW Spain)". Food Control 59 (2016): 759-765. http://dx.doi.org/10.1016/j.foodcont.2015.06.054.
    10.1016/j.foodcont.2015.06.054
  35. Garrido-Maestu, Alejandro. "Re-evaluation of Enhanced qPCR Prevalidated Method for Next-day Detection of Salmonella spp., Shigella spp., Escherichia coli O157 and Listeria monocytogenes". Food Biotechnology (2015):
    10.1080/08905436.2015.1091977
  36. Garrido-Maestu, Alejandro. "Detection of foodborne pathogens by qPCR: A practical approach for food industry applications". Cogent Food & Agriculture (2015):
    10.1080/23311932.2015.1013771
  37. Garrido-Maestu, A.; Vieites-Maneiro, R.; Peñaranda, E.; Cabado, A.G.. "Development, and complete evaluation, of a novel Most-Probable-Number (MPN) qPCR method for accurate and express quantification of Listeria monocytogenes in foodstuffs". European Food Research and Technology (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84937949148&partnerID=MN8TOARS.
    10.1007/s00217-015-2496-9
  38. Garrido-Maestu, A.; Chapela, M.-J.; Vieites, J.M.; Cabado, A.G.. "LolB gene, a valid alternative for qPCR detection of Vibrio cholerae in food and environmental samples". Food Microbiology 46 (2015): 535-540. http://www.scopus.com/inward/record.url?eid=2-s2.0-84908370411&partnerID=MN8TOARS.
    10.1016/j.fm.2014.09.012
  39. Atanassova, M.R.; Chapela, M.-J.; Garrido-Maestu, A.; Fajardo, P.; Ferreira, M.; Lago, J.; Aubourg, S.P.; Vieites, J.M.; Cabado, A.G.. "Microbiological quality of ready-to-eat pickled fish products". Journal of Aquatic Food Product Technology 23 5 (2014): 498-510. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905740559&partnerID=MN8TOARS.
    10.1080/10498850.2012.731676
  40. Garrido-Maestu, A.; Chapela, M.-J.; Peñaranda, E.; Vieites, J.M.; Cabado, A.G.. "In-house validation of novel multiplex real-time PCR gene combination for the simultaneous detection of the main human pathogenic vibrios (Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus)". Food Control 37 1 (2014): 371-379. http://www.scopus.com/inward/record.url?eid=2-s2.0-84886785245&partnerID=MN8TOARS.
    10.1016/j.foodcont.2013.09.026
  41. Fajardo, P.; Atanassova, M.; Garrido-Maestu, A.; Wortner-Smith, T.; Cotterill, J.; Cabado, A.G.. "Bacteria isolated from shellfish digestive gland with antipathogenic activity as candidates to increase the efficiency of shellfish depuration process". Food Control 46 (2014): 272-281. http://www.scopus.com/inward/record.url?eid=2-s2.0-84902439413&partnerID=MN8TOARS.
    10.1016/j.foodcont.2014.05.038
  42. Garrido-Maestu, A.; Chapela, M.-J.; Vieites, J.M.; Cabado, A.G.. "Application of real-time PCR to detect Listeria monocytogenes in a mussel processing industry: Impact on control". Food Control 46 (2014): 319-323. http://www.scopus.com/inward/record.url?eid=2-s2.0-84902469118&partnerID=MN8TOARS.
    10.1016/j.foodcont.2014.05.048
  43. Garrido-Maestu, A.; Chapela, M.-J.; Román, B.; Vieites, J.M.; Cabado, A.G.. "Application of a novel pathogenicity marker in a multiplex real-time PCR method to assess total and pathogenic Vibrio vulnificus in food and environmental samples". Food Control 35 1 (2014): 274-283. http://www.scopus.com/inward/record.url?eid=2-s2.0-84881260268&partnerID=MN8TOARS.
    10.1016/j.foodcont.2013.07.007
  44. Garrido-Maestu, Alejandro. "In-house validation of a multiplex real-time PCR method for simultaneous detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes". International Journal of Food Microbiology (2013):
    doi:10.1016/j.ijfoodmicro.2013.03.024
  45. Garrido-Maestu, Alejandro. "In Vitro Approaches To Evaluate Toxicity Induced by Organotin Compounds Tributyltin (TBT), Dibutyltin (DBT), and Monobutyltin (MBT) in Neuroblastoma Cells". Journal of Agricultural and Food Chemistry (2013):
    10.1021/jf3050186
  46. Garrido-Maestu, Alejandro. "A new multiplex real-time PCR developed method for Salmonella spp. and Listeria monocytogenes detection in food and environmental samples". Food Control (2013):
    doi:10.1016/j.foodcont.2012.06.029
  47. Garrido-Maestu, Alejandro. "Development of a multiplex real-time PCR method for pathogenic Vibrio parahaemolyticus detection (tdh+ and trh+)". Food Control (2012):
    doi:10.1016/j.foodcont.2011.09.015
  48. Garrido-Maestu, Alejandro. "Development of a multiplex real-time PCR method for simultaneous detection of Salmonella enterica , Shigella flexneri and Listeria monocytogenes in processed food samples". European Food Research and Technology (2012):
    10.1007/s00217-012-1665-3
  49. Garrido-Maestu, Alejandro. "Cytotoxic activity of extracts of marine sponges from NW Spain on a neuroblastoma cell line". Environmental Toxicology and Pharmacology (2011):
    doi:10.1016/j.etap.2011.08.012
  50. Garrido-Maestu, Alejandro. "Comparison between a TaqMan Polymerase Chain Reaction Assay and a Culture Method for ctx-Positive Vibrio cholerae Detection". Journal of Agricultural and Food Chemistry (2010):
    10.1021/jf903658k
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2019/04/24 Optimized Methodology for Same-day Detection of E. coli O157 by Multiplex qPCR in Ground Meat and Leafy Greens IAFP's European Symposium
International Association for Food Protection (Nantes, France)
2018/06/19 Isothermal DNA amplification strategies for the detection of Listeria monocytogenes in food samples 40th International Conference on Environmental & Food Monitoring
(Santiago de Compostela, Spain)
2017/11/29 Isothermal DNA amplification for Salmonella spp. detection and characterization 2nd International Conference on Food Microbiology
(Madrid, Spain)
2016/09/14 Detection of Salmonella spp. by Loop-mediated isothermal AMPlification, combined with gold nanoparticles XX National Conference on Food Microbiology
Spanish Society for Microbiology (Leon, Spain)
2011/11/01 Vibrio 2011. Development of a Real-Time PCR method for the detection of patho-genic V. parahaemolyticus in seafood and water samples The fourth conference on the biology of Vibrios
(Santiago de Compostela, Spain)
2010/09/14 Comparison of real time PCR and ISO 21872-1:2007 for the detection of pathogenic Vibrio parahaemolyticus VIII Meeting of the Microbiology of Aquatic Medium
Spanish Society of Microbiology (Vigo, Spain)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2019/03/02 - Current Fast molecular methods for the detection of spoilage fungi (in food products)
Co-supervisor of Foteini Roumani
International Iberian Nanotechnology Laboratory, Portugal

Universidade de Santiago de Compostela, Spain
2017/03/02 - Current Fast, Affordable and multiplexed foodborne pathogen detection on miniaturized devices
Co-supervisor
International Iberian Nanotechnology Laboratory, Portugal

Universidade de Santiago de Compostela, Spain

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2019/07/01 Understanding DNA-DNA interactions using graphene transistors
(Thesis) Main arguer
 Telma Campos Domingues (Master)
International Iberian Nanotechnology Laboratory, Portugal

Universidade do Minho, Portugal
2016/11/25 Development and evaluation of Loop-Mediated Isothermal Amplification (LAMP) for Listeria monocytogenes detection in food samples
(Thesis) Arguer
 Joana Filipa Pinto Ramos (Other)
Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal

Association member

Society Organization name Role
2014 - Current Spanish Society for Microbiology
2006 - Current Official College of Biologists of Galicia

Journal scientific committee

Journal title (ISSN) Publisher
2020/03/09 - Current Food Analytical Methods (1936-976X) Springer-Verlag
2020 - Current Comprehensive Reviews in Food Science and Food Safety (1541-4337) Wiley (Blackwell Publishing)
2020 - Current Experimental Parasitology (1090-2449) Elsevier
2020 - Current Journal of Visualized Experiments (1940-087X) MyJoVE Corporation
2019/12/20 - Current Foods (2304-8158) MDPI AG
2019 - Current Food Microbiology (1095-9998) Elsevier
2019 - Current Food Research International (0963-9969) Elsevier
2019 - Current Journal of Microbiological Methods (0167-7012) Elsevier
2019 - Current Journal of Veterinary Medicine and Animal Health (2141-2529) Academic Journals
2018 - Current Transactions of the American Society of Agricultural and Biological Engineers (2151-0032)
2018 - Current BMC Microbiology (1471-2180) Springer (Biomed Central Ltd.)
2018 - Current Canadian Journal of Microbiology (1480-3275) Canadian Science Publishing
2018 - Current FEMS Microbiology Letters (1574-6968) Oxford University Press
2018 - Current Food Chemistry (0308-8146) Elsevier
2018 - Current Frontiers in Microbiology (1664-302X) Frontiers Media SA
2017 - Current African Journal of Microbiology Research (1996-0808) Academic Journals
2017 - Current Cogent Food & Agriculture (2331-1932) Informa UK (Taylor & Francis)
2017 - Current Ecosistemas y Recursos Agropecuarios (2007-901X) Universidad Juarez Autonoma de Tabasco
2017 - Current Food Analytical Methods (1936-976X) Springer-Verlag
2017 - Current Food Biotechnology (1532-4249) Informa UK (Taylor & Francis)
2017 - Current Food Control (0956-7135) Elsevier
2017 - Current International Journal of Food Microbiology (0168-1605) Elsevier
2017 - Current Journal of Food Safety (1745-4546) Wiley (Blackwell Publishing)
2017 - Current Journal of Photochemistry and Photobiology B Biology (1011-1344) Elsevier
2017 - Current SOJ Microbiology & Infectious Diseases (2372-0956) Symbiosis Group
2017 - Current SpringerPlus (2193-1801) Springer (Biomed Central Ltd.)
2014 - Current Journal of Dairy Science (1525-3198) American Dairy Science Association
Distinctions

Award

2020 IBAB Indutry Award
2013 Extraordinary Thesis Award
Universidade de Vigo, Spain