Isabel Calejo (1A1F-B26C-15CC)

Curriculum Vitae

Isabel Calejo

Isabel Calejo graduated with a degree in Bioengineering from the Catholic University of Porto in 2012. She began her research career at the Lab of Pharmacology and Neurobiology (ICBAS-UP) as a trainee, contributing to hands-on teaching for MSc students. In 2015, she completed her MSc in Forensic Sciences - Toxicology at FMUP, focusing on metabolomic profiling via GC-MS to differentiate urine samples from diseased (prostate cancer) and healthy individuals, leading to her first publication as a first author. In 2016, Isabel was awarded a PhD scholarship for the Tissue Engineering, Stem Cells and Regenerative Medicine at the University of Minho, 3B's Research Group. Her thesis, defended in May 2021, explored the engineering of tendon-to-bone interface through gradient scaffolds, culminating in 5 original articles, 2 reviews, and 2 book chapters. Concurrently, she enhanced her networking and leadership skills through an international mentoring program with the Director of Bioinformatics at BenevolentAI, England. Nonetheless, her work was recognized abroad by receiving a travel grant for attending an international conference. Before completing her PhD, Isabel started a postdoc at the University of Twente (Netherlands), focusing on microfluidic chips for real-time biomarker monitoring in collaboration with ERASMUS MC, resulting in a co-authored publication (10.1016/j.bios.2023.115510). Also, in 2021, she joined the i3S Bioengineered 3D Microenvironments Group (Porto) as a postdoctoral fellow, investigating endothelial plasticity to enhance therapeutic angiogenesis for ischemic cardiac tissues, where she developed her skills on microtissues fabrication and hydrogels synthesis. Later, in 2023, she joined the "Cello" project (FMUP, Porto), working on label-free methodologies for biomarkers detection. Isabel has excelled in competitive grant writing, securing over €20,000 in funding. Notable awards include a Kick-Starter grant for fabricating 3D biotextiles for skeletal muscle regeneration (2022) and the EORS Grant Tank competition for her "MuSculpt - Magnetic sculpting of vascularized skeletal muscle tissue" project (2023). As an expert reviewer for European Commission (H2020 Europe), Isabel provides critical evaluations for high-impact projects. Additionally, she has authored/co-authored >20 scientific communications, 16 articles ( 14 articles, 2 reviews and 2 book chapters). She also serves as a reviewer for top journals (Bioac. Mat., MDPI, ACS Biomaterials) and served as Guest Editor for Bioengineering (MDPI) and Co-Editor at Frontiers. Over the years, with a commitment to mentoring and supervision, Isabel has supervised MSc and PhD students and also participated in academic examinations, being the principal jury in two MSCs defenses: "Development of fibrous structures based on nanocellulose wetspun microfibers for tendon and ligament repair, healing an regeneration” and "Polymeric-based devices such as medical adhesives – from biomaterials to biological characterization tests". Overall, her expertise spans from biomaterials, microfabrication, cell biology, bioinformatics (Python, machine learning), to intellectual property management, underscored by advanced training in research project management and life sciences entrepreneurship.

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Identification

Personal identification

Full name: Isabel Calejo

Citation names

Calejo, Isabel
Calejo, I.

Author identifiers

Ciência ID: 1A1F-B26C-15CC
ORCID iD: 0000-0002-6015-0655
Researcher Id: S-3103-2016

Email addresses

icalejo@med.up.pt (Professional)

Websites

https://orcid.org/0000-0002-6015-0655 (Professional)
https://www.linkedin.com/in/isabelcalejo/ (Professional)
https://www.researchgate.net/profile/Isabel-Calejo (Professional)

Knowledge fields

Engineering and Technology - Materials Engineering
Engineering and Technology - Nano-technology
Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism
Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
English	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)
Portuguese	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)	Proficiency (C2)

Education

	Degree	Classification
2023/05 Concluded	Molecular Bioinformatics - Hands-on Course Online, 9th Edition (Pós-Graduação) Major in Bioinformatics Universidade do Porto Faculdade de Medicina, Portugal	17
2023/03 - 2023/03 Concluded	INTRODUCTION TO PYTHON AND MACHINE LEARNING FOR THE BIOSCIENCES, 5TH EDITION (Outros) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022/11 - 2022/11 Concluded	LIFE SCIENCE INTELECTUAL PROPERTY: MANAGEMENT AND EXPLOITATION (Outros) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022/09 - 2022/09 Concluded	EIT HEALTH RIS ACADEMY - Empowering women's entrepreneurship in health (Outros) Universidad de Navarra IESE Business School, Spain
2022/01 Concluded	RESEARCH PROJECT MANAGEMENT (Outros) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2016 - 2021 Concluded	Engenharia de Tecidos, Medicina Regenerativa e Células Estaminais (Doutoramento) Universidade do Minho - 3Bs Research Group, Portugal "Engineering the tendon-to-bone tissue interface mimicking its microenvironment: from stem cells to gradient scaffolds." (THESIS/DISSERTATION)
2019/11 - 2020/11 Concluded	Programa internacional de mentorias em Biomedicina – Desenvolvimento de Soft Skills (Outros) Major in Soft skills International Mentoring Foundation for the Advancement of Higher Education, Spain
2016/07 Concluded	Curso de Laboratório em Ciências Animais (equivalente à categoria C FELASA) (Outros) Centro de investigação médica, Faculdade de Medicina, Universidade do Porto (CIM-FMUP), Portugal
2015 Concluded	Ciências Forenses (Mestrado) Major in Toxicologia Forense Universidade do Porto Faculdade de Medicina, Portugal "Development of a GC-MS method for determination of volatile carbonyl compounds in biological matrices: aplication to clinical and post-mortem samples" (THESIS/DISSERTATION)
2012/10/03 Concluded	Bioengenharia (Licenciatura) Major in Engenharia Biomédica Universidade Católica Portuguesa Escola Superior de Biotecnologia, Portugal ""Testes acelerados para adesividade de adesivos médicos"¿ " (THESIS/DISSERTATION)	-

Affiliation

Science

	Category Host institution	Employer
2024 - Current	Contracted Researcher (Research)	International Iberian Nanotechnology Laboratory, Portugal
	International Iberian Nanotechnology Laboratory, Portugal
2023/09 - 2024	Contracted Researcher (Research)	Universidade do Porto Faculdade de Medicina, Portugal
	Centro de investigação médica, Faculdade de Medicina, Universidade do Porto (CIM-FMUP), Portugal
2021/09/01 - 2023	Postdoc (Research)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021/04/01 - 2021/09/01	Postdoc (Research)	Universiteit Twente, Netherlands
	Universiteit Twente Technical Medical Centre, Netherlands (...)
2016/09 - 2021/05/07	Researcher (Research)	Universidade do Minho - 3Bs Research Group, Portugal
	Universidade do Minho, Portugal
2016/01 - 2016/07	Research Trainee (Research)	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal (...)
2012/03 - 2014/04	Research Trainee (Research)	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal (...)

Others

	Category Host institution	Employer
2022/01 - 2023	Editor Convidado - Special Issue "Next Generation Bioengineered Strategies for Musculoskeletal Regeneration	MDPI AG, Switzerland

2019/11 - 2020/09	Representante dos alunos de doutoramento da unidade I3Bs	i3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Portugal
	i3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Portugal
2012 - 2012	Estágio Curricular	BASTOS VIEGAS, S.A., Portugal
	BASTOS VIEGAS, S.A., Portugal

Projects

Grant

	Designation	Funders
2016/09 - 2020/10	Mimicking tendon-to-bone interface: from stern cells to phasic scaffolds Provided by PTCRIS: PD/BD/128088/2016	Fundação para a Ciência e a Tecnologia
2012/03 - 2014/04	P2 Purinoceptors in human osteogenesis and bone disease SFRH/BD/68584/2010 Research Trainee Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Contract

	Designation	Funders
2023/09 - Current	MuSculpt - Magnetic sculpting of vascularized skeletal muscle tissue MuSculpt Principal investigator Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Orthoregeneration Network Foundation (ON) Ongoing
2023/05 - Current	MyoTextile - Living cell-based 3D biotextiles for skeletal muscle tissue regeneration Myotextile Principal investigator Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Orthoregeneration Network Foundation (ON) Ongoing
2021/01/01 - 2025/12/31	ICVS/3B¿s - Laboratório Associado, Instituto de Ciências da Vida e da Saúde / Grupo de Investigação em Biomateriais, Biodegradaveis e Biomiméticos LA/P/0050/2020 10.54499/LA/P/0050/2020 UID/Multi/50026/2019 Universidade do Minho, Portugal Universidade do Minho Instituto de Investigação em Ciências da Vida e Saúde, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2021/03/29 - 2024/03/28	Harnessing endothelial plasticity for therapeutic vascularization of ischemic tissues: a bioengineering approach PTDC/BTM-ORG/5154/2020 Post-doc Fellow Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2021/04/01 - 2021/09/01	Towards osteoarthritis fingerprinting combining imaging biomarkers and multi-organ-on-chip technology for improved in vitro models OA-BioDetectChips Post-doc Universiteit Twente - Developmental Bioengineering Group, Netherlands Erasmus MC Biomedical Imaging Group Rotterdam, Netherlands	Samenwerkende Gezondheidsfondsen (SGF) Ongoing
2016/09/01 - 2020/10/31	Mimicking tendon-to-bone interface: from stem cells to phasic scaffolds PD/BD/128088/2016 PhD Student Fellow Universidade do Minho, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2019/01/01 - 2019/12/31	Unidade de Ciências Biomoleculares Aplicadas UID/Multi/04378/2019 154717UID Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Other

	Designation	Funders
2013 - 2014	Pathophysiological role of purines (ATP and adenosine) in the human bladder hyperactivity PTDC/SAU-OSM/104369/2008 Research Trainee Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal	Fundação para a Ciência e a Tecnologia, I.P. & COMPETE 2020 Concluded

Outputs

Publications

Book chapter	Isabel Calejo; Marcel Alexander Heinrich; Giorgia Zambito; Laura Mezzanotte; Jai Prakash; Liliana Moreira Teixeira. "Advancing Tumor Microenvironment Research by Combining Organs-on-Chips and Biosensors". 2022. 10.1007/978-3-031-04039-9_7 Calejo, Isabel; Costa-Almeida, Raquel; Gomes, Manuela E.. "Cellular Complexity at the Interface: Challenges in Enthesis Tissue Engineering". In Cell Biology and Translational Medicine, Volume 5, 71-90. Springer International Publishing, 2019. Published • 10.1007/5584_2018_307
Conference abstract	Domingues, Rui M. A.; Calejo, Isabel; Claudia J. Labrador-Rached,; Gomez-Florit, Manuel; Denitsa Docheva; Reis, L. Rui; Manuela E. Gomes. "In vitro modelling of tendon physiology and pathology with 3D microengineered living fibers.". Paper presented in ESB 2021, 2021. Noronha-Matos, JB; Calejo, Isabel; Maria Teresa Magalhães-Cardoso; Isabel Silva; MA Costa; Fatima Ferreirinha; R Freitas; et al. "NTPDase3 enzyme inhibition or gene silencing rescues the osteogenic potential of post-menopausal mesenchymal stein cells (MSCs) derived from hone marrow". Paper presented in PURINERGIC SIGNALLING, 2018. Published Silva, Isabel; Costa, AF; Moreira, Sílvia; Ferreirinha, F; Magalhães-Cardoso, MT; Calejo, I; Silva-Ramos, Miguel; et al. "Downmodulation of cholinergic neurotransmission by ß3-adrenoceptor agonists depends on adenosine release and cyclic AMP activation of the exchange protein EPAC in the human rat urinary bladder.". Paper presented in 42nd FEBS CONGRESS ¿ FROM MOLECULES TO CELLS AND BACK. JERUSALEM, ISRAEL, 2017. Published Silva, Isabel; Costa, AF; Ferreirinha, F; Magalhães-Cardoso, MT; Calejo, Isabel; Silva-Ramos, Miguel; Correia-de-Sá, P; et al. "Adenosine release and A1 receptors activation mediate the inhibitory effect of ß3-adrenoceptors in the human and rat urinary bladder.". Paper presented in XIX NATIONAL CONGRESS OF BIOCHEMISTRY. GUIMARÃES, PORTUGAL, 2016. Published Calejo, Isabel; Nathalie Moreira; Araújo, Ana Margarida; Carvalho, M; C. Moreira-Barbosa; Carmen Jerónimo; Rui Henrique; Bastos, ML; Guedes de Pinho, Paula. "Development of an analytical method by HS-SPME-GC/MS for volatile carbonyl biomarkers discovery in urines from patients with prostate cancer". Paper presented in JUP 2015 - 8º Encontro de Jovens Investigadores da U.PORTO, 2015.
Conference paper	Calejo, I.; Almeida, Raquel Carvalho Ferreira Costa; Reis, R. L.; Gomes, Manuela E.. "Oxygen conditioning effect on an in vitro co-culture model of tendon-to-bone interface". 2019. Accepted Calejo, Isabel; Costa-Almeida, Raquel; Reis, Rui L.; Manuela E. Gomes. "Topological and compositional gradients to generate 3D textured living microfibres resembling tendon-to-bone interface". Paper presented in 27th Annual Meeting of the European Orthopaedic Research Society (EORS), Maastricht, 2019. Published Almeida, Raquel Carvalho Ferreira Costa; Calejo, I.; Domingues, Rui Miguel Andrade; Reis, R. L.; Gomes, Maria Manuela Estima. "Platelet lysate cell-laden hydrogel-coated suture threads for tendon repair". Paper presented in EORS - European Orthopedic Research Society (EORS) 2018 - 26th Annual Meeting, 2018. Published • 10.1302/1358-992X.2018.16.124 Calejo, I.; Almeida, Raquel Carvalho Ferreira Costa; Reis, R. L.; Gomes, Manuela E.. "Optimization and establishment of a co-culture model to study cellular interactions in tendon-to-bone interface". Paper presented in EORS - 25th Meeting, 2017. Published • 10.1302/1358-992X.2018.3.070 I. Silva; A. F. Costa; S. Moreira; F. Ferreirinha; M. Teresa Magalhães-Cardoso; Calejo, Isabel; M. Silva-Ramos; P. Correia-de-Sá. "Beta3-adrenoceptors inhibit cholinergic neurotransmission in the bladder indirectly via ADO release and A1 receptor activation". Paper presented in ISN-ENS 2017 meeting, 2017. Published "Organotypic-like tendon explant co-culture systems to study the crosstalk between native tendon cells and adipose stem cells". Paper presented in European Orthopaedic Research Society (EORS) 25th Annual Meeting,, 2017. Accepted Calejo, I.; Moreira, N.; Araújo, A.M.; Carvalho, M.; Moreira-Barbosa, C.; Jerónimo, C.; Henrique, R.; Bastos, M.L.; Guedes de Pinho, P.. "Development of an analytical method with PFBHA derivatization followed by headspace SPME-GC/MS for the determination of urinary volatile carbonyl metabolites in patients with prostate cancer". 2015. Published • 10.1016/j.toxlet.2015.08.686 Calejo, Isabel; José Bernardo Almeida Garrett de Noronha Matos. "Adult bone marrow stromal cells expressing ecto-NTPDase3 may be a novel therapeutic target for defective osteogenesis and bone mineralization". 2014. Published • 10.1007/s11302-014-9430-7 "Abstracts from Purines 2014, an International Conference on Nucleotides, Nucleosides and Nucleobases, held in Bonn, Germany, from July 23–27, 2014.". 2014.
Conference poster	Graça, Ana L.; Domingues, R.M.A.; Calejo, I.; Gomez-Florit, M.; Gomes, M.E.. "Platelet-derived Extracellular Vesicles Show Therapeutic Effects on a 3D Tendon Disease Model 32nd Annual Conference of the European Society for Biomaterials.". Paper presented in 32nd Annual Conference of the European Society for Biomaterials, 2022. "Platelet-derived Extracellular Vesicles Show Therapeutic Effects on a 3D Tendon Disease Model". Paper presented in Third Achilles Workshop “Gene & Cell Therapy and clinical applications”, 2021. Calejo, Isabel; Costa-Almeida, Raquel; Reis, R. L.; Manuela E. Gomes. "Textured microfibers with topological and compositional gradients resembling tendon- to-bone interface". Paper presented in 1st Discoveries Forum on Regenerative and Precision Medicine, 2019. "Multi-compartment multifunctional composite living fibers for tendon tissue engineering and regeneration". Paper presented in TERMIS European Chapter Meeting 2019, 2019. "Engineering continuous textured composite microfibers for tendon-to-bone mimicry". Paper presented in TERMIS European Chapter Meeting 2019, 2019. Calejo, Isabel; Costa-Almeida, Raquel; Reis, R. L.; Manuela E. Gomes. "Hypoxia conditioning effect on an in vitro tendon-to-bone interface co-culture model". Paper presented in Gene2Skin/CHEM2NATURE Final Conference, 2018. Calejo, Isabel; Costa-Almeida, Raquel; Ana Isabel Gonçalves; Dominika Berdecka; Reis, Rui L.; Manuela E. Gomes. "Unraveling the crosstalk between pre-osteoblasts and tendon cells in tendon-to-bone regeneration". Paper presented in 5th TERMIS World Congress, 2018. Calejo, Isabel; Costa-Almeida, Raquel; Reis, Rui L.; Manuela E. Gomes. "Wet-spinning technique for the production of PCL/Gel and PCL/Gel/nHA continuous fibers for tendon-to-bone insertion regeneration". Paper presented in Chem2Nature/Gene2skin – Summer School, 2018. R. Costa-Almeida; Calejo, Isabel; R. Altieri; R.M.A. Domingues; E. Giordano; R.L. Reis; M.E. Gomes. "Suture threads coated with cell-laden platelet lysate hydrogel for tendon applications". Paper presented in CHEM2NATURE Final Conference, 2018. "PLATELET LYSATE HYDROGEL-COATED SUTURE THREADS FOR TENDON TISSUE ENGINEERED APPROACHES". Paper presented in Summer School Chem2Nature, 2018. Calejo, Isabel; Costa-Almeida, Raquel; Reis, Rui L.; Manuela E. Gomes. "Bi-directional crosstalk between tendon cells and pre-osteoblasts: a co-culture approach for tendon-to-bone interfacial tissue engineering". Paper presented in TERMSTEM/FORECAST 2017 - 1o Workshop of FoReCaST -The Tumour Microenvironment, 2017. Calejo, Isabel; Costa-Almeida, Raquel; Reis, Rui L.; Manuela E. Gomes. "Co-culture model to study the role of cellular interactions in enthesis". Paper presented in CHEM2NATURE Second school, 2017. I. Silva; A. F. Costa; S. Moreira; F. Ferreirinha; M. Teresa Magalhães-Cardoso; Calejo, Isabel; M. Silva-Ramos; P. Correia-de-Sá. "Downmodulation of cholinergic neurotransmission by ß3-adrenoceptor agonists depends on adenosine release and cyclic AMP activation of the exchange protein Epac in the human and rat urinary bladder". Paper presented in 42nd Federation of European Biochemical Societies Congress - From Molecules to Cells and Back, 2017. "Adipose stem cells and native tendon cells as partners in orchestrating tendon regeneration: in vitro co-culture approaches". Paper presented in FORECAST - 1st Workshop, 2017. Calejo, Isabel; Ana Margarida Araújo;; Nathalie Moreira; Jerónimo, Carmen; Rui Henrique; Bastos, Maria de Lourdes; Márcia Carvalho; Guedes de Pinho, Paula. "A GC-MS methodology for the determination of potential urinary volatile carbonyl as biomarkers of prostate cancer". Paper presented in I Encontro Anual do Departamento de Ciências Biológicas da Faculdade de Farmácia da Universidade do Porto, 2016. Calejo, Isabel; Moreira, Nathalie; Ana Margarida Araújo; Carvalho, Márcia; Bastos, Maria de Lourdes; Guedes de Pinho, Paula. "Development and optimisation of a GC methodology with PFBHA derivatization for the determination of volatile carbonyl metabolites in urine of smokers and non-smokers". Paper presented in I Encontro Anual do Departamento de Ciências Biológicas da Faculdade de Farmácia da Universidade do Porto, 2016. Calejo, Isabel; Nathalie Moreira; Ana Margarida Araujo; Marcia Carvalho; C. Moreira-Barbosa; Carmem jerónimo; Rui Henrique; M. Lourdes Bastos; Guedes de Pinho, P.. "Development of an analytical method with PFBHA derivatization followed by headspace SPME GC/MS for the determination of urinary volatile carbonyl metabolites in patients with prostate cancer". Paper presented in 51st Congress of the European Societies of Toxicology (EUROTOX), 2015.
Journal article	Orge, I.D.; Nogueira Pinto, H.; Silva, M.A.; Bidarra, S.J.; Ferreira, S.A.; Calejo, I.; Masereeuw, R.; Mihaila, S.M.; Barrias, C.C.. "Vascular units as advanced living materials for bottom-up engineering of perfusable 3D microvascular networks". Bioactive Materials 38 (2024): 499-511. http://dx.doi.org/10.1016/j.bioactmat.2024.05.021. Published • 10.1016/j.bioactmat.2024.05.021 Sofia M. Costa; Leandro M. O. Lourenço; Ricardo C. Calhelha; Isabel Calejo; Cristina C. Barrias; Raul Fangueiro; Diana P. Ferreira. "Localized cancer photodynamic therapy approach based on core–shell electrospun nanofibers". Materials Advances (2024): https://doi.org/10.1039/D4MA00647J. 10.1039/D4MA00647J Nuno Araújo-Gomes; Giorgia Zambito; Castro Johnbosco; Isabel Calejo; Jeroen Leijten; Clemens Löwik; Marcel Karperien; Laura Mezzanotte; Liliana Moreira Teixeira. "Bioluminescence imaging on-chip platforms for non-invasive high-content bioimaging". Biosensors and Bioelectronics (2023): https://doi.org/10.1016/j.bios.2023.115510. 10.1016/j.bios.2023.115510 Calejo, Isabel; Labrador-Rached, Claudia J.; Gomez-Florit, Manuel; Docheva, Denitsa; Reis, Rui L.; Domingues, Rui M. A.; Gomes, Manuela E.. "Bioengineered 3D Living Fibers as In Vitro Human Tissue Models of Tendon Physiology and Pathology". Advanced Healthcare Materials 11 15 (2022): http://dx.doi.org/10.1002/adhm.202102863. 10.1002/adhm.202102863 Ana L. Graça; Rui M. A. Domingues; Isabel Calejo; Manuel Gómez-Florit; Manuela E. Gomes. "Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model". International Journal of Molecular Sciences 23 6 (2022): 2948-2948. https://doi.org/10.3390/ijms23062948. 10.3390/ijms23062948 Calejo, Isabel; Reis, Rui L.; Domingues, Rui M. A.; Gomes, Manuela E.. "Texturing Hierarchical Tissues by Gradient Assembling of Microengineered Platelet-Lysates Activated Fibers". Advanced Healthcare Materials (2021): 2102076. http://dx.doi.org/10.1002/adhm.202102076. Published • 10.1002/adhm.202102076 Calejo, Isabel; Costa-Almeida, Raquel; Reis, Rui L.; Gomes, Manuela E.. "In vitro temporal HIF-mediated deposition of osteochondrogenic matrix governed by hypoxia and osteogenic factors synergy". Journal of Cellular Physiology 236 5 (2020): 3991-4007. http://dx.doi.org/10.1002/jcp.30138. Published • 10.1002/jcp.30138 Calejo, Isabel; Costa-Almeida, Raquel; Reis, Rui L.; Gomes, Manuela E.. "A Physiology-Inspired Multifactorial Toolbox in Soft-to-Hard Musculoskeletal Interface Tissue Engineering". Trends in Biotechnology 38 1 (2020): 83-98. http://dx.doi.org/10.1016/j.tibtech.2019.06.003. Published • 10.1016/j.tibtech.2019.06.003 Calejo, Isabel; Costa-Almeida, Raquel; Reis, Rui L.; Gomes, Manuela E.. "Enthesis Tissue Engineering: Biological Requirements Meet at the Interface". Tissue Engineering Part B: Reviews 25 4 (2019): 330-356. http://dx.doi.org/10.1089/ten.teb.2018.0383. Published • 10.1089/ten.teb.2018.0383 Costa-Almeida, Raquel; Calejo, Isabel; Gomes, Manuela E.. "Mesenchymal Stem Cells Empowering Tendon Regenerative Therapies". International Journal of Molecular Sciences 20 12 (2019): 3002. http://dx.doi.org/10.3390/ijms20123002. Published • 10.3390/ijms20123002 Calejo, Isabel; Costa-Almeida, Raquel; Reis, Rui L.; Gomes, Manuela E.. "A Textile Platform Using Continuous Aligned and Textured Composite Microfibers to Engineer Tendon-to-Bone Interface Gradient Scaffolds". Advanced Healthcare Materials 8 15 (2019): 1900200. http://dx.doi.org/10.1002/adhm.201900200. Published • 10.1002/adhm.201900200 Costa-Almeida, Raquel; Calejo, Isabel; Altieri, Roberta; Domingues, Rui M A; Giordano, Emanuele; Reis, Rui L; Gomes, Manuela E. "Exploring platelet lysate hydrogel-coated suture threads as biofunctional composite living fibers for cell delivery in tissue repair". Biomedical Materials 14 3 (2019): 034104. http://dx.doi.org/10.1088/1748-605x/ab0de6. Published • 10.1088/1748-605x/ab0de6 Calejo, I.; Costa-Almeida, Raquel; Gonçalves, Ana Isabel; Berdecka, Dominika; Reis, Rui Luis; Gomes, Manuela Estima. "Bi-directional modulation of cellular interactions in an in vitro co-culture model of tendon-to-bone interface". Cell Proliferation 51 6 (2018): e12493. http://dx.doi.org/10.1111/cpr.12493. Published • 10.1111/cpr.12493 Costa-Almeida, Raquel; Calejo, Isabel; Reis, Rui L.; Gomes, Manuela E.. "Crosstalk between adipose stem cells and tendon cells reveals a temporal regulation of tenogenesis by matrix deposition and remodeling". Journal of Cellular Physiology 233 7 (2018): 5383-5395. http://dx.doi.org/10.1002/jcp.26363. 10.1002/jcp.26363 Silva, Isabel; Costa, Ana Filipa; Moreira, Sílvia; Ferreirinha, Fátima; Magalhães-Cardoso, Maria Teresa; Calejo, Isabel; Silva-Ramos, Miguel; Correia-de-Sá, Paulo. "Inhibition of cholinergic neurotransmission by ß3-adrenoceptors depends on adenosine release and A1-receptor activation in human and rat urinary bladders". American Journal of Physiology-Renal Physiology 313 2 (2017): F388-F403. http://dx.doi.org/10.1152/ajprenal.00392.2016. Published • 10.1152/ajprenal.00392.2016 Silva, I.; Costa, A. F.; Moreira, S.; Ferreirinha, F.; Magalhaes-Cardoso, M. T.; Calejo, I.; Silva-Ramos, M.; Correia-de-Sa, P.. "Downmodulation of cholinergic neurotransmission by beta 3-adrenoceptor agonists depends on adenosine release and cyclic AMP activation of the exchange protein Epac in the human and rat urinary bladder". The FEBS Journal (2017): https://publons.com/publon/19988441/. Calejo, Isabel; Moreira, Nathalie; Araújo, Ana Margarida; Carvalho, Márcia; Bastos, Maria de Lourdes; de Pinho, Paula Guedes. "Optimisation and validation of a HS-SPME-GC-IT/MS method for analysis of carbonyl volatile compounds as biomarkers in human urine: Application in a pilot study to discriminate individuals with smoking habits". Talanta 148 (2016): 486-493. http://dx.doi.org/10.1016/j.talanta.2015.09.070. Published • 10.1016/j.talanta.2015.09.070
Thesis / Dissertation	Calejo, Isabel. "Engineering the tendon-to-bone tissue interface mimicking its microenvironment: from stem cells to gradient scaffolds". PhD, Universidade do Minho - 3Bs Research Group, 2021. https://repositorium.sdum.uminho.pt/handle/1822/75229. Helena Isabel Pinto Cardoso Teixeira Calejo. "Development of a GC-MS method for determination of volatile carbonyl compounds in biological matrices: aplication to clinical and post-mortem samples". Master, 2015. https://repositorio-aberto.up.pt/handle/10216/88980.

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2021/09/06	In vitro modelling of tendon physiology and pathology with 3D microengineered living fibers	ESB 2021 (Porto, Portugal)
2019	Textured microfibers with topological and compositional gradients resembling tendon-to-bone interface	1st Discoveries Forum on Regenerative and Precision Medicine (Porto, Portugal)
2019	Topological and compositional gradients to generate 3D textured living microfibers resembling tendon-to-bone interface	European Orthopaedic Research Society (EORS) - 27th Annual Meeting (Maastricht, Netherlands)
2019	Hypoxia mediated response in an in vitro co-culture model of tendon-to-bone interface	1st Achilles Conference – Molecular and biological mechanisms of tendon homeostasis and repair (Porto, Portugal)
2019	Oxygen conditioning effect on an in vitro co-culture model of tendon-to-bone interface	TERMIS European Chapter Meeting 2019 (Rhodes, Greece)
2018	Platelet lysate cell-laden hydrogel-coated suture threads for tendon repair	European Orthopaedic Research Society (EORS) 26th Annual Meeting (Galway, Ireland)
2017	Optimization and establishment of a co-culture model to study cellular interactions in tendon-to-bone interface	European Orthopaedic Research Society (EORS) 25th Annual Meeting, (Munich, Germany)
2016	GC/MS analysis of carbonyl volatile compounds as human urinary biomarkers: Discrimination between smoking and non-smoking individuals	Sociedade Portuguesa Farmacologia
2015	Development of an analytical method by HS-SPME-GC/MS for volatile carbonyl biomarkers discovery in urines from patients with prostate cancer	IJUP 2015 - 8º Encontro de Jovens Investigadores da U.PORTO (Porto, Portugal)

Event organisation

	Event name Type of event (Role)	Institution / Organization
2019/10/04 - 2019/10/05	Participated in sessions organization and discussion after oral presentations of key speakers of 2nd Achilles Conference - Tendon biophysical environment¿ (2019/10/04 - 2019/10/05) Conference (Co-organisor)	Universidade do Minho - 3Bs Research Group, Portugal
2019/07/08 - 2019/07/10	Participated in sessions organization and discussion after oral presentations of key speakers at 1st Achilles Conference - Molecular and biological mechanisms of tendon homeostasis and repair¿ (2019/07/08 - 2019/07/10) Conference (Co-organisor)	Universidade do Minho - 3Bs Research Group, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2020/06 - Current	Training seminar on Quality System ISO 9001:2015	Quality Management System on ISO 9001:2015 Universidade do Minho - 3Bs Research Group, Portugal

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2023	Polymeric-based devices such as medical adhesives – from biomaterials to biological characterization tests (Thesis) Main arguer	Mariana Pereira Conceição (Master) Universidade Católica Portuguesa Centro Regional do Porto, Portugal
2022	Development of fibrous structures based on nanocellulose wetspun microfibers for tendon and ligament repair, healing and regeneration (Thesis) Main arguer	Joana Filipa Magalhães da Rocha (Master) Universidade do Minho, Portugal

Association member

	Society Organization name	Role
2017 - Current	European Orthopaedic Research Society (EORS)	Membership

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2016 - 2016	Technical assistance of practical lessons: "Renal excretion of Aspirin metabolites" and "Effect of a loop diuretic in the rabbit: Furosemide"	Human Medicine, Veterinary and Biochemistry (Master)	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Evaluation committee

	Activity description Role	Institution / Organization	Funding entity
2021 - Current	Kick-start grant Evaluator	Orthoregeneration Network Foundation (ON), Switzerland	Orthoregeneration Network Foundation (ON)

Journal scientific committee

	Journal title (ISSN)	Publisher
2022 - Current	Bioactive Materials	Elsevier
2021 - Current	Bioengineering (2306-5354)	MDPI
2020 - Current	ACS Biomaterials Science & Engineering	ACS Publications
2020 - Current	Tissue Engineering Part A (1937-3341)	Mary Ann Liebert

Scientific expedition

	Activity description	Institution / Organization
2022/01 - Current	Special Issue "Next Generation Bioengineered Strategies for Musculoskeletal Regeneration".	MDPI AG, Switzerland

Distinctions

Award

2023	ON/EORS Kick Grant Tank Competition
2022	ON/ORS Kick-Starter Grants 2022/23 Orthoregeneration Network Foundation (ON), Switzerland
2019	ON/EORS Travel Grant Orthoregeneration Network Foundation (ON), Switzerland
2019	StartUP Voucher

Other distinction

2016	PhD scholarship Fundação para a Ciência e a Tecnologia, Portugal