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Graciano Silva Leal. Concluiu o(a) Doutoramento em Biociências em 2014 pelo(a) Universidade de Coimbra Faculdade de Ciencias e Tecnologia e Licenciatura em Biologia em 2008 pelo(a) Universidade de Coimbra Faculdade de Ciencias e Tecnologia. É Investigador Contratado no(a) Instituto de Medicina Molecular João Lobo Antunes. Publicou 8 artigos em revistas especializadas. Possui 1 capítulo(s) de livros. Recebeu 2 prémio(s) e/ou homenagens. Atua na(s) área(s) de Ciências Naturais com ênfase em Ciências Biológicas com ênfase em Biologia Celular. No seu currículo Ciência Vitae os termos mais frequentes na contextualização da produção científica, tecnológica e artístico-cultural são: Nucleus; Cell excitability; Contraction; .
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Identification

Personal identification

Full name
Graciano Silva Leal

Citation names

  • Leal, Graciano

Author identifiers

Ciência ID
1C10-08E8-6A4A
ORCID iD
0000-0001-6333-6095

Knowledge fields

  • Natural sciences - Biological Sciences - Cell Biology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2014
Concluded
 Biociências (Doutoramento)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Regulation of hnRNP A2/B1 and hnRNP K by Synaptic Activity and BDNF in the Hippocampus" (THESIS/DISSERTATION)
 Highest distintion
2008
Concluded
 Biologia (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"n/a" (THESIS/DISSERTATION)
 16
Affiliation

Science

Category
Host institution 		Employer
2019/02/01 - Current Postdoc (Research) Instituto de Medicina Molecular João Lobo Antunes, Portugal
2016/05/16 - 2019/02/01 Postdoc (Research) Instituto de Medicina Molecular João Lobo Antunes, Portugal
2015/06/01 - 2015/10/31 Postdoc (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2014/07/01 - 2015/05/31 Postdoc (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2014/03/01 - 2014/06/30 Research Assistant (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2010/01/01 - 2013/12/31 Researcher (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2012/05/05 - 2012/12/12 Visiting Researcher (Research) Department of Biomedicine and K.G. Jebsen Centre for Research on Neuropsychiatric Disorders, University of Bergen, Norway
2008/10/01 - 2009/12/31 Research Assistant (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2007/09/01 - 2008/07/01 Research Trainee (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Projects

Grant

Designation Funders
2015 - 2015 Alterations in synaptic signaling and in synaptic protein synthesis in Temporal Lobe Epilepsy: possible role for hnRNP K
Bolsa Cientifica da LPCE 2015
Principal investigator
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
 Liga Portuguesa Contra a Epilepsia
2010/01/01 - 2013/12/31 REGULATION OF HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS (HNRNPS) BY THE NEUROTROPHIN BDNF AND SYNAPTIC PLASTICITY
info:eu-repo/grantAgreement/FCT/SFRH/SFRH2%FBD2%F664162%F2009/PT
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
 Fundação para a Ciência e a Tecnologia

Contract

Designation Funders
2019/02/01 - Current NuSkeMus - Positioning the nucleus in skeletal muscle cells
-
Researcher
Instituto de Medicina Molecular João Lobo Antunes, Portugal
 Fundação para a Ciência e a Tecnologia
2016/05/01 - 2019/02/01 PhoNICs - Positioning the nucleus for cell migration and muscle fiber function
617676
Post-doc Fellow
Instituto de Medicina Molecular João Lobo Antunes, Portugal
 European Research Council
2015/06/01 - 2015/10/30 Downregulation of the GABAergic synaptic transmission in brain ischemia - molecular mechanisms of GABAA receptor internalization
info:eu-repo/grantAgreement/FCT/COMPETE/124339/PT
Post-doc Fellow
Universidade do Algarve Faculdade de Ciências e Tecnologia
2014/07/01 - 2015/05/01 CENTRO-07-ST24-FEDER-002002 - Mais Centro - “New strategies to manage brain diseases"
CENTRO-07-ST24-FEDER-002002
Post-doc Fellow
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
 Fundação para a Ciência e a Tecnologia
2014/03/01 - 2014/06/30 Regulation of the ubiquitin-proteasome system by BDNF in hippocampal synapses: role in synaptic plasticity
PTDC/SAU-NMC/120144/2010
Research Technician Fellow
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
 Fundação para a Ciência e a Tecnologia
2008/10/01 - 2009/12/31 Regulation of the hnRNP proteins by the neurotrophin BDNF: role in synaptic plasticity
PTDC/SAU-NEU/104297/2008
Research Technician Fellow
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
 Fundação para a Ciência e a Tecnologia
2007/11/01 - 2008/10/31 Mechanisms of synaptic plasticity and neuroprotection by BDNF in the hippocampus: inhibition of neurodegeneration vs regeneration
PTDC/SAU-FCF/72283/2006
Other
Fundação para a Ciência e a Tecnologia
Outputs

Publications

Book chapter
  1. Leal, Graciano. "BDNF and Hippocampal Synaptic Plasticity". In Neurotrophins. 2017.
Journal article
  1. Singer, Wibke; Manthey, Marie; Panford-Walsh, Rama; Matt, Lucas; Geisler, Hyun-Soon; Passeri, Eleonora; Baj, Gabriele; et al. "BDNF-Live-Exon-Visualization (BLEV) Allows Differential Detection of BDNF Transcripts in vitro and in vivo". Frontiers in Molecular Neuroscience 11 (2018): http://dx.doi.org/10.3389/fnmol.2018.00325.
    10.3389/fnmol.2018.00325
  2. Leal, Graciano. "The RNA-Binding Protein hnRNP K Mediates the Effect of BDNF on Dendritic mRNA Metabolism and Regulates Synaptic NMDA Receptors in Hippocampal Neurons". eNeuro (2017):
  3. Leal, Graciano. "Role of GABAA R trafficking in the plasticity of inhibitory synapses.". (2016):
  4. Leal, Graciano; Afonso, Pedro M.; Salazar, Ivan L.; Duarte, Carlos B.. "Regulation of hippocampal synaptic plasticity by BDNF". Brain Research 1621 (2015): 82-101. http://dx.doi.org/10.1016/j.brainres.2014.10.019.
    10.1016/j.brainres.2014.10.019
  5. Leal, G.; Comprido, D.; Duarte, C.B.. "BDNF-induced local protein synthesis and synaptic plasticity". Neuropharmacology 76 PART C (2014): 639-656. http://www.scopus.com/inward/record.url?eid=2-s2.0-84890442031&partnerID=MN8TOARS.
    10.1016/j.neuropharm.2013.04.005
  6. Leal, G.; Afonso, P.M.; Duarte, C.B.. "Neuronal activity induces synaptic delivery of hnRNP A2/B1 by a BDNF-dependent mechanism in cultured hippocampal neurons". PLoS ONE 9 10 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84907698379&partnerID=MN8TOARS.
    10.1371/journal.pone.0108175
  7. Caldeira, M.V.; Curcio, M.; Leal, G.; Salazar, I.L.; Mele, M.; Santos, A.R.A.; Melo, C.V.; et al. "Excitotoxic stimulation downregulates the ubiquitin-proteasome system through activation of NMDA receptors in cultured hippocampal neurons". Biochimica et Biophysica Acta - Molecular Basis of Disease 1832 1 (2013): 263-274. http://www.scopus.com/inward/record.url?eid=2-s2.0-84868560715&partnerID=MN8TOARS.
    10.1016/j.bbadis.2012.10.009
  8. Baptista, M.S.; Melo, C.V.; Armelão, M.; Herrmann, D.; Pimentel, D.O.; Leal, G.; Caldeira, M.V.; et al. "Role of the proteasome in excitotoxicity-induced cleavage of glutamic acid decarboxylase in cultured hippocampal neurons". PLoS ONE 5 4 (2010): http://www.scopus.com/inward/record.url?eid=2-s2.0-77956333111&partnerID=MN8TOARS.
    10.1371/journal.pone.0010139
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2019/05/19 Regulation of excitation-contraction coupling in skeletal muscle by nuclei positioning Muscle: Excitation-Contraction Coupling (GRS)
Gordon Research Conferences (Lucca (Barga), Italy)
2017/05/06 Functional role of peripheral nuclei in the regulation of contraction and voltage-gated sodium channels localization in skeletal muscle Muscle: Excitation-Contraction Coupling (GRC)
Gordon Research Conferences (Les Diablerets, Switzerland)
2017/02/23 Regulation of skeletal muscle function by nuclei positioning Career development and soft skills for young scientists
ReTuBi - Towards outstanding research and training in tumour biology at IMM (Lisboa, Portugal)
2013/12/19 Regulation of hnRNP K and hnRNP K-associated mRNAs by synaptic activity CNC annual meeting
CNC (Cantanhede, Portugal)
2013/09/25 Regulation of hnRNP K and hnRNP K-associated mRNAs by synaptic activity ISN/FENS School - Local protein synthesis in axons and dendrites
ISN/FENS (Kolymbari, Greece)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2014/01/02 - 2014/11/30 Activity-dependent changes in the dendritic distribution of hnRNP K: functional implication
Co-supervisor of Eduardo Manuel Firmo Morais
Cellular and Molecular Biology (Master)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2018/05/01 - 2018/06/30 Whitman Research Assistant at the Marine Biological Laboratory
Other
Marine Biological Laboratory, United States
2018/04/22 - 2018/04/27 Regulation of excitation-contraction coupling in skeletal muscle by nuclei positioning
Conference
 Muscle Development, Regeneration and Disease 2018
Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft, Germany
2015/06/04 - 2015/06/05 The RNA-binding protein hnRNP K is regulated by synaptic activity and BDNF in the hippocampus: implications for mRNA metabolism in dendrites
Meeting
 XIV meeting of the Portuguese Society for Neuroscience
Sociedade Portuguesa de Neurociências, Portugal
2014/05/01 - 2014/05/04 The RNA-binding protein hnRNP K acts downstream of TrkB signaling to regulate mRNA metabolism in dendrites
Conference
 ISN 2016 ISN 2016 Special Conference of Neurochemistry “Synaptic Function and Dysfunction in Brain Diseases”
International Society for Neurochemistry, Switzerland

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2019/07/08 - 2019/07/20 Physiology: Modern Cell Biology Using Microscopic, Biochemical and Computational Approaches The MBL phisiology course (Postgraduate Certificate) Marine Biological Laboratory, United States
2016/04/20 - 2016/04/20 Synaptic Modulation (Neurotrophins) Neuroscience (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Distinctions

Award

2019 JGP Knox Chandler Award
The Rockefeller University Press, United States
2007 Merit award for top 3% of students (University of Coimbra)
Universidade de Coimbra, Portugal