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I have a degree in Chemical Engineering from Coimbra University (1989), obtained a Ph.D. in Chemical Engineering (1993) and the Habilitation in Chemical Engineering (2003) from Instituto Superior Técnico (IST), and was a visiting scholar at the Massachusetts Institute of Technology in 1997 and 2007. I joined the Chemical Engineering Department of IST in 1993 as an Assistant Professor, was promoted to Associate Professor in 2001 and to Full Professor in 2015. I am currently affiliated with the Department of Bioengineering and with the Institute for Bioengineering and Biosciences (iBB) at IST. I am currently affiliated with the Department of Bioengineering and with the Institute for Bioengineering and Biosciences (iBB) at IST. As a senior researcher in the Biomolecular and Bioprocess Engineering fields, I have been addressing two major topics: Biomanufacturing of Nucleic Acids, and Biosensing and Diagnostics. I coordinated several national research projects that secured funding in these areas. I am the author of 215 papers, 25 book chapters, 3 books and 1 US patent. I am especially known for my work/expertise on DNA manufacturing. I have supervised (15) and co-supervised (15) doctoral thesis in Bioengineering and in Biotechnology. At iBB I coordinate the Bioprocess Engineering Research group (approx. 10 PhD holders), which is focused on exploring new engineering approaches, address unmet technological challenges and translate knowledge in molecular and cellular mechanisms into efficient bioprocesses and applications that are relevant to the Human Health and Bioeconomy sectors. I've extensively taught various engineering subjects to undergraduates/graduates in Biological Engineering, Biomedical Engineering, and Biotechnology, including Transport Phenomena, Bioseparations, Bioprocess Engineering, Cell and Gene Therapy, Biomimetics and Bioengineering Labs. Additionally, I've instructed on non-engineering topics like Innovation in Healthcare for students in these fields. I have held several managerial positions at IST. I was vice-president of the Bioengineering Department (2019-2022), and a member of the Pedagogical (2013-2015) and the School Councils (2015.-2020). I am a member of the Scientific Council (since 2021), where I serve as vice-president, and of the Ethics Committee (since 2022) of IST. I have been a member of the Executive committee of iBB and coordinator of the Bioengineering Research group since 2015.
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Identification

Personal identification

Full name
Duarte Miguel de França Teixeira dos Prazeres

Citation names

  • Prazeres, Duarte
  • Prazeres, DMF

Author identifiers

Ciência ID
1D1D-6C27-D99D
ORCID iD
0000-0002-6928-692X
Researcher Id
E-2526-2011
Scopus Author Id
7005220457

Websites

Knowledge fields

  • Engineering and Technology - Industrial Biotechnology - Bioprocessing Technologies
  • Engineering and Technology - Industrial Biotechnology - Industrial Biotechnology

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2003
Concluded
 Engenharia Química (Título de Agregado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
 Approved by unanimity
1993
Concluded
 Engenharia Química (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Estudo de Reactores Enzimáticos com Lipase Microencapsulada" (THESIS/DISSERTATION)
 Approved by unanimity with distinction
1989
Concluded
 Engenharia Química (Licenciatura) (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
 16 valores
Affiliation

Teaching in Higher Education

Category
Host institution 		Employer
2015/01/01 - Current Full Professor (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal
2001/06/01 - 2014/12/01 Associate Professor (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto Superior Técnico, Portugal
1993/02/01 - 2001/06/01 Assistant Professor (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto Superior Técnico, Portugal
Projects

Grant

Designation Funders
2018/09 - 2021/09 CBM-X: Biorecognition as a Tool for the Functionalization of Cellulose-based Materials with Biomolecules and Nanostructures
PTDC/CTM-CTM/30790/2017
 Fundação para a Ciência e a Tecnologia
2018/06 - 2021/05 On2OffNanomagnets: Development and application of nanomagnets and ferrofluids with an "on-to-off" variation of magnetization around room-temperature
02/SAICT/2017/029460
 Fundação para a Ciência e a Tecnologia
2020/07 - 2020/12 LFA4Covid: Development of a Lateral Flow Assay working prototype for detection of antibodies to SARS-CoV-2
Research COVID 19-576
 Fundação para a Ciência e a Tecnologia
2013/04 - 2015/03 Functionalized Metal Nano-Gaps for Plasmon-Enhanced Single Protein Biosensing
Provided by PTCRIS: 128608
128608
 Fundação para a Ciência e a Tecnologia, I.P.
2014/03 - 2015/02 Avian influenza hemagglutinin subunit DNA vaccines with targeting sequences in frame with DIVA strategy
133382
Provided by PTCRIS: 133382
 Fundação para a Ciência e a Tecnologia, I.P.
2012/01 - 2014/12 DSPMab - Intensification of the downstream process of monoclonal antibodies
117427
Provided by PTCRIS: 117427
 Fundação para a Ciência e a Tecnologia, I.P.
2011/04 - 2014/03 How "green" is a constructed wetland?
112032
Provided by PTCRIS: 112032
 Fundação para a Ciência e a Tecnologia, I.P.
2011/01 - 2013/12 Melhoramento da imunogenicidade de vacinas de DNA
113650
Provided by PTCRIS: 113650
 Fundação para a Ciência e a Tecnologia, I.P.
2010/02 - 2013/07 Membrane chromatographic matrices for plasmid-DNA purification: rational design based on biochemical and biophysical interactions
100925
Provided by PTCRIS: 100925
 Fundação para a Ciência e a Tecnologia, I.P.
2010/01 - 2013/03 Immunotherapy of African trypanosomiasis in the mouse model by DNA vaccination
102486
Provided by PTCRIS: 102486
 Fundação para a Ciência e a Tecnologia, I.P.
2010/01 - 2012/12 A Lab-on-a-chip-based 2D fluorescence detection system for organism/cell condition analysis (Micro-2D-FS)
104387
Provided by PTCRIS: 104387
 Fundação para a Ciência e a Tecnologia, I.P.
2009/08 - 2011/07 Integrated Lab On Chip Platforms for Medical Diagnostics
Provided by PTCRIS: 109367
109367
 Fundação para a Ciência e a Tecnologia, I.P.
2007/10 - 2010/12 Synthesis, optimization and intensification of bioseparation processes for DNA vaccine manufacturing
Provided by PTCRIS: 65492
65492
 Fundação para a Ciência e a Tecnologia, I.P.
2007/08 - 2010/12 USE OF THE VACCINE ADJUVANT FOR IMMUNOMODULATION OF THE INFECTION CAUSED BY TRYPANOSOMA BRUCEI: AN APPROACH TO VACCINE DESIGN
72624
Provided by PTCRIS: 72624
 Fundação para a Ciência e a Tecnologia, I.P.
2005/02 - 2008/01 Development of experimental DNA vaccines against sheep Maedi-Visna virus
Provided by PTCRIS: 47260
 Fundação para a Ciência e a Tecnologia, I.P.
2005/07 - 2007/12 Development of DNA vaccine prototypes for veterinary trypanosomiasis
Provided by PTCRIS: 61090
61090
 Fundação para a Ciência e a Tecnologia, I.P.
2005/07 - 2007/12 Overcoming the nucleolytic barrier during trafficking of plasmid DNA vectors
55799
Provided by PTCRIS: 55799
 Fundação para a Ciência e a Tecnologia, I.P.
2005/07 - 2007/10 Electronically-addressed BioChips (eBioArray)
Provided by PTCRIS: 60487
60487
 Fundação para a Ciência e a Tecnologia, I.P.
2005/01 - 2007/06 Electronic Detection of Biomolecules for Microarray Applications
58636
Provided by PTCRIS: 58636
 Fundação para a Ciência e a Tecnologia, I.P.
2004/01 - 2007/06 Molecular therapy agents preparation by simultaneous purification and condensation of plasmids in aqueous two-phase systems
Provided by PTCRIS: 47245
 Fundação para a Ciência e a Tecnologia, I.P.
2002/10 - 2005/12 Micro-electromechanical resonators: high sensitivity DNA detectors (Bio-MEMS)
Provided by PTCRIS: 37719
 Fundação para a Ciência e a Tecnologia, I.P.

Contract

Designation Funders
2023/03/01 - 2026/02/28 A Gold Nanosensor for Single-Molecule Detection of RNA Biomarkers in a Miniaturized Microscope
2022.04076.PTDC
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2020/10/01 - 2023/06/30 Functionalized nanoparticles for enrichment and efficient detection of SARS-CoV-2 in<br>clinical and environmental samples
SAICTCOVID/72536/2020
Universidade de Lisboa Instituto Superior Técnico, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2018/08/10 - 2022/12/31 Uma abordagem nanotecnológica para administração tópica de insulina para cicatrização de feridas
PTDC/MEC-DER/32610/2017
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2018/10/01 - 2022/04/30 Lactic acid bacteria as cell factories: a Synthetic Biology approach for plasmid DNA and recombinant protein production
PTDC/BTM-SAL/28624/2017
Provided by PTCRIS: PTDC/BTM-SAL/28624/2017
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book
  1. Prazeres, Duarte. Exercícios e Cálculos Introdutórios em Engenharia de Bioprocessos. IST Press. 2022.
    Published
  2. Prazeres, Duarte. Plasmid Biopharmaceuticals: Basics, Applications and Manufacturing. New York: John Wiley & Sons. 2011.
    Published
  3. Prazeres, Duarte; de Pinho, Maria Norberta. Fundamentos de Transferência de Massa. IST Press. 2006.
    Published
Book chapter
  1. Martins, Sofia Aires M.; Prazeres, Duarte Miguel F.; Chu, Virginia; Conde, João P.. "Monitoring Intracellular Calcium in Response to GPCR Activation: Comparison Between Microtiter Plates and Microfluidic Assays". In G Protein-Coupled Receptor Screening Assays, 289-304. Springer US, 2021.
    10.1007/978-1-0716-1221-7_19
  2. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "Purificação de Plasmídeos". In Purificação de Produtos Biotecnológicos: Operações e Processos com Aplicação Industrial, 641-672. São Paulo,, Brazil: Edgard Blücher Ltda, 2020.
  3. Wagner, Alexandra; Silva-Santos, A. Rita; Rosa, Sara Sousa; Gierak, Sophie; Azevedo, Ana M.; Prazeres, Duarte Miguel F.. "Primary Purification of Plasmid DNA Using Differential Isopropanol Precipitation". In Methods in Molecular Biology, 151-165. Springer US, 2020.
    10.1007/978-1-0716-0872-2_8
  4. Silva-Santos, A. Rita; Rosa, Sara Sousa; Prazeres, Duarte Miguel F.; Azevedo, Ana M.. "Purification of Plasmid DNA by Multimodal Chromatography". In Methods in Molecular Biology, 193-205. Springer US, 2020.
    10.1007/978-1-0716-0872-2_10
  5. Prazeres, Duarte. "Use of Enzymes in the Downstream Processing of Biopharmaceuticals". In Pharmaceutical Biocatalysis: Fundamentals, Enzyme Inhibitors, and Enzymes in Health and Diseases, edited by P. Grunwald, 41-72. Singapore, Singapore: Pan Stanford Publishing Pte. Ltd, 2019.
    Published • 10.1201/9780429295034-2
  6. Prazeres, Duarte; Pereira, P. M. M.; Monteiro, G. A.; Prazeres, D. M. F.. "General Aspects of Biomimetic Materials". In Biotechnologies and Biomimetics for Civil Engineering, edited by Pacheco Torgal, Fernando; Labrincha, A. J.; Diamanti, V. M.; Yu, C.-P.; Lee, K. H., 57-79. Springer International Publishing, 2015.
    10.1007/978-3-319-09287-4_3
  7. Prazeres, Duarte. "Use of ImageJ to Recover Information from Individual Cells in a G-Protein Coupled Receptor Assay". In GPCR Screening Assays: Methods and Protocols. Methods in Molecular Biology., 143-172. New York: Springer Science+Business Media, 2015.
    Published • 10.1007/978-1-4939-2336-6_11
  8. Prazeres, Duarte; Gabriel António Amaro Monteiro. "Plasmids Biopharmaceuticals". In Plasmids: Biology and Impact in Biotechnology and Discovery, 669-688. Washington DC, United States: ASM Press, 2015.
    Published
  9. Prazeres, Duarte Miguel F.; Martins, Sofia Aires M.; Prazeres, Duarte. "G protein-Coupled Receptors: An Overview of Signaling Mechanisms and Screening Assays". In G Protein-Coupled Receptor Screening Assays, 3-19. Springer New York, 2015.
    Published
  10. Freitas EB; Henriques AM; Fevereiro, M.; Prazeres, Duarte; Monteiro, Gabriel António Amaro. "Enhancement of DNA vaccine efficacy by intracellular targeting strategies". In DNA Vaccines: Methods and Protocols. Methods in Molecular Biology, edited by Rinaldi, Monica; Fioretti, Daniela, 33-59. New York, United States: Springer Science+Business Media, 2014.
    Published • 10.1007/978-1-4939-0410-5_4
  11. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "Chromatographic Separation of Plasmid DNA Using Macroporous Beads". In Macroporous Polymers, 335-361. CRC Press, 2009.
    Published • 10.1201/9781420084627-c13
  12. Prazeres, Duarte Miguel F.; Monteiro, Gabriel Amaro. "DNA Vaccines". In A Portrait of State-of-the-Art Research at the Technical University of Lisbon, 219-232. Springer Netherlands, 2007.
    10.1007/978-1-4020-5690-1_14
  13. Freitas, Sindélia S.; Santos, José A. L.; Prazeres, D. Miguel F.. "Plasmid DNA". In Development of Sustainable Bioprocesses, 271-285. John Wiley & Sons, Ltd, 2006.
    10.1002/9780470058916.ch15
  14. Diogo, Maria Margarida; Queiroz, João Ant'onio; Prazeres, Duarte Miguel F.. "Purification of Plasmid DNA Vectors Produced in Escherichia coli for Gene Therapy and DNA Vaccination Applications". In Microbial Processes and Products, 165-178. Humana Press, 2005.
    10.1385/1-59259-847-1:165
  15. Azevedo, Ana M.; Martins, Verónica C.; Prazeres, Duarte M.F.; Vojinovic, Vojislav; Cabral, Joaquim M.S.; Fonseca, Luís P.; Azevedo, A.M.; et al. "Horseradish peroxidase: a valuable tool in biotechnology". In Biotechnology Annual Review, 199-247. Elsevier, 2003.
    10.1016/s1387-2656(03)09003-3
  16. Ferreira, Guilherme N. M.; Prazeres, Duarte M. F.; Cabral, Joaquim M. S.; Schleef, Martin. "Plasmid Manufacturing– An Overview". In Plasmids for Therapy and Vaccination, 193-236. Wiley-VCH Verlag GmbH, 2001.
    10.1002/9783527612833.ch11
  17. Prazeres, Duarte. "Temperature, pH and Media Influence on Lipase Stability". 445-450. Elsevier, 1993.
    10.1016/b978-0-444-89372-7.50058-0
  18. Prazeres, Duarte. "Batch and Continuous Lipolysis/Product Separation in a Reversed Micellar Membrane Bioreactor". 713-718. Elsevier, 1992.
    10.1016/b978-0-444-89046-7.50102-8
Conference abstract
  1. Fantoni, Alessandro; Fernandes, Miguel; Vygranenko, Yuri; Vieira, Manuela; Alegria, Elisabete; Ribeiro, Ana; Prazeres, Duarte; Silva, Rui P.. "Optical properties of metal nanoparticles embedded in amorphous silicon analysed using discrete dipole approximation". 2018.
    10.1117/12.2289983
Conference paper
  1. Brogueira, Pedro; Goncalves, Antonio Pereira; Prazeres, DMF; Marrucho, Isabel; Bioucas, Jose; Nunes, Joao Pimentel; Correia, Joao Ramoa; et al. "Técnico2122: rethinking engineering education at IST". 2021.
    10.1109/cispee47794.2021.9507237
  2. Prazeres, D M F; Martins, S A M; Trabuco, J R C; Monteiro, G A; Juskowiak, A; Conde, J P; Chu, V. "Towards a high-throughput drug discovery platform for the screening of GPCR targets in cells". 2011.
    10.1109/enbeng.2011.6026042
  3. Mateus, Marilia; Raiado-Pereira, Luis; Prazeres, Miguel. "Membrane chromatography for therapeutic DNA production: Adsorption membranes development". 2011.
    10.1109/enbeng.2011.6026054
  4. Joskowiak, A.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Amorphous Silicon Photosensors for Detection of Intrinsic Cell Fluorophores". 2011.
    Published • 10.1557/opl.2011.1230
Edited book
  1. Prazeres, Duarte; Martins, Sofia Aires M.. G Protein-Coupled Receptor Screening Assays. Methods and Protocols. Springer Science+Business Media. 2021.
    Published • Editor
  2. Prazeres, Duarte; Martins, Sofia AM. G Protein-Coupled Receptor Screening Assays. Methods and Protocols. New York: Springer Science+Business Media. 2015.
    Published • Editor
Encyclopedia entry
  1. Prazeres, D. M. F.; Santos, J. A. L.. "Production and Purification of Adenovirus Vectors for Gene Therapy". In Pharmaceutical Sciences Encyclopedia. John Wiley & Sons, Inc., 2010. http://dx.doi.org/10.1002/9780470571224.pse329.
    10.1002/9780470571224.pse329
Journal article
  1. Rui Oliveira-Silva; Yuyang Wang; Sjoerd W. Nooteboom; Duarte M. F. Prazeres; Pedro M. R. Paulo; Peter Zijlstra. "Single-Particle Plasmon Sensor to Monitor Proteolytic Activity in Real Time". ACS Applied Optical Materials (2023): https://doi.org/10.1021/acsaom.3c00226.
    10.1021/acsaom.3c00226
  2. Satheesh Natarajan; Jayaraj Joseph; Duarte Miguel França Prazeres. "Graphene oxide coatings enhance fluorescence signals in a lateral flow immunoassay for the detection of UCH-L1, a marker for trauma brain injury". Sensors and Actuators B: Chemical (2023): http://dx.doi.org/10.1016/j.snb.2023.134336.
    10.1016/j.snb.2023.134336
  3. Prazeres, DMF. Corresponding author: Prazeres, DMF. "The supporting role of plasmids in gene and cell therapy". Cell and Gene Therapy Insights 09 05 (2023): 755-762. http://dx.doi.org/10.18609/cgti.2023.094.
    Published • 10.18609/cgti.2023.094
  4. Jorge João; Duarte Miguel F. Prazeres. "Manufacturing of non-viral protein nanocages for biotechnological and biomedical applications". Frontiers in Bioengineering and Biotechnology (2023): http://dx.doi.org/10.3389/fbioe.2023.1200729.
    10.3389/fbioe.2023.1200729
  5. Sara Sousa Rosa; Davide Nunes; Luis Antunes; Duarte M. F. Prazeres; Marco P. C. Marques; Ana M. Azevedo. "Maximizing mRNA vaccine production with Bayesian optimization". Biotechnology and Bioengineering 119 11 (2022): 3127-3139. http://dx.doi.org/10.1002/bit.28216.
    10.1002/bit.28216
  6. Silva-Santos, A. Rita; Paulo, Pedro M.R.; F. Prazeres, Duarte Miguel. "Scalable purification of single stranded DNA scaffolds for biomanufacturing DNA-origami nanostructures: Exploring anion-exchange and multimodal chromatography". Separation and Purification Technology 298 (2022): 121623. http://dx.doi.org/10.1016/j.seppur.2022.121623.
    10.1016/j.seppur.2022.121623
  7. Natarajan, Satheesh; Joseph, Jayaraj; França Prazeres, Duarte Miguel. "Exploring carbohydrate binding module fusions and Fab fragments in a cellulose-based lateral flow immunoassay for detection of cystatin C". Scientific Reports 12 1 (2022): http://dx.doi.org/10.1038/s41598-022-09454-9.
    10.1038/s41598-022-09454-9
  8. Mariana Barbosa; Hélvio Simões; Sandra N. Pinto; Ana S. Macedo; Pedro Fonte; D.Miguel F. Prazeres. "Fusions of a Carbohydrate Binding Module with the Small Cationic Hexapeptide RWRWRW Confer Antimicrobial Properties to Cellulose-based Materials". Acta Biomaterialia (2022): https://doi.org/10.1016/j.actbio.2022.02.042.
    10.1016/j.actbio.2022.02.042
  9. A. Rita Silva-Santos; Rui Oliveira-Silva; Sara Sousa Rosa; Pedro M. R. Paulo; Duarte Miguel F. Prazeres. "Affinity-Based Magnetic Particles for the Purification of Single-Stranded DNA Scaffolds for Biomanufacturing DNA-Origami Nanostructures". ACS Applied Nano Materials (2021): https://doi.org/10.1021/acsanm.1c03623.
    10.1021/acsanm.1c03623
  10. Cláudia P.A. Alves; Duarte Miguel F. Prazeres; Gabriel A. Monteiro. "Recombination efficiency measurement by real-time PCR: A strategy to evaluate ParA-mediated minicircle production". Analytical Biochemistry 628 (2021): 114285-114285. https://doi.org/10.1016/j.ab.2021.114285.
    10.1016/j.ab.2021.114285
  11. Joana Serra; Cláudia P. A. Alves; Joaquim M. S. Cabral; Gabriel A. Monteiro; Cláudia L. Silva; Duarte Miguel F. Prazeres. "Minicircle-based expression of vascular endothelial growth factor in mesenchymal stromal cells from diverse human tissues". The Journal of Gene Medicine (2021): https://doi.org/10.1002/jgm.3342.
    10.1002/jgm.3342
  12. Mariana Barbosa; Hélvio Simões; Duarte Miguel F. Prazeres. "Functionalization of Cellulose-Based Hydrogels with Bi-Functional Fusion Proteins Containing Carbohydrate-Binding Modules". Materials 14 12 (2021): 3175-3175. https://doi.org/10.3390/ma14123175.
    10.3390/ma14123175
  13. Satheesh Natarajan; Joseph Jayaraj; Duarte Miguel F. Prazeres. "A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I". Biosensors 11 2 (2021): 49-49. https://doi.org/10.3390/bios11020049.
    10.3390/bios11020049
  14. Prazeres, Duarte. "Minicircle Biopharmaceuticals – an Overview of Purification Strategies". Frontiers Chemical Engineering (2021):
    10.3389/fceng.2020.612594
  15. Prazeres, Duarte. "Monitoring Proteolytic Activity in Real Time: A New World of Opportunities for Biosensors". Trends in Biochemical Sciences (2020): http://dx.doi.org/10.1016/j.tibs.2020.03.011.
    10.1016/j.tibs.2020.03.011
  16. Prazeres, Duarte; David Botequim; Inês I. R. Silva; Sofia G. Serra; Eduardo P. Melo; Duarte M. F. Prazeres; Sílvia M. B. Costa; Pedro M. R. Paulo. "Fluorescent dye nano-assemblies by thiol attachment directed to the tips of gold nanorods for effective emission enhancement". Nanoscale 12 11 (2020): 6334-6345. http://dx.doi.org/10.1039/d0nr00267d.
    10.1039/d0nr00267d
  17. Prazeres, Duarte. "Ionic Liquid-Polymer Nanoparticle Hybrid Systems as New Tools to Deliver Poorly Soluble Drugs". Nanomaterials (2019): http://dx.doi.org/10.3390/nano9081148.
    10.3390/nano9081148
  18. Ana M. M. Rosa; Maria R. Nazaré; Duarte M. F. Prazeres. "Colorimetric Detection of DNA Strands on Cellulose Microparticles Using ZZ–CBM Fusions and Gold Nanoparticles". Biotechnology Journal 14 8 (2019): 1800590-1800590. https://doi.org/10.1002/biot.201800590.
    10.1002/biot.201800590
  19. Ana M. M. Rosa; Maria R. Nazaré; Duarte M. F. Prazeres. "Back Cover Picture: Biotechnology Journal 8/2019". Biotechnology Journal (2019): https://doi.org/10.1002/biot.201970084.
    10.1002/biot.201970084
  20. Prazeres, Duarte. "Plasmid Copy Number of pTRKH3 in Lactococcus lactis is Increased by Modification of the repDE Ribosome-Binding Site". Biotechnology Journal (2019): http://dx.doi.org/10.1002/biot.201800587.
    10.1002/biot.201800587
  21. Rui Oliveira-Silva; Mariana Sousa-Jerónimo; David Botequim; Nuno J. O. Silva; Duarte M. F. Prazeres; Pedro M. R. Paulo. "Density Gradient Selection of Colloidal Silver Nanotriangles for Assembling Dye-Particle Plasmophores". Nanomaterials (2019): https://doi.org/10.3390/nano9060893.
    10.3390/nano9060893
  22. Prazeres, Duarte. "Extreme Enhancement of Single-Molecule Fluorescence from Porphyrins Induced by Gold Nanodimer Antennas". The Journal of Physical Chemistry Letters (2019): http://dx.doi.org/10.1021/acs.jpclett.9b00373.
    10.1021/acs.jpclett.9b00373
  23. Prazeres, Duarte. "Multimodal Chromatography of Supercoiled Minicircles: A Closer Look into DNA-ligand Interactions". Sep. Pur. Technol. (2019):
    10.1016/j.seppur.2018.11.015
  24. Serra, Joana; Alves, Cláudia P.A.; Brito, Liliana; Monteiro, Gabriel A.; Cabral, Joaquim M.S.; Prazeres, Duarte Miguel F.; da Silva, Cláudia L.. "Engineering of Human Mesenchymal Stem/Stromal Cells with Vascular Endothelial Growth Factor–Encoding Minicircles for Angiogenic Ex Vivo Gene Therapy". Human Gene Therapy (2018): http://dx.doi.org/10.1089/hum.2018.154.
    10.1089/hum.2018.154
  25. Pandey, Rajat; Kumar, Nitin; Monteiro, Gabriel A.; Veeranki, Venkata Dasu; Prazeres, D.M.F.. "Re-engineering of an Escherichia coli K-12 strain for the efficient production of recombinant human Interferon Gamma". Enzyme and Microbial Technology 117 (2018): 23-31. http://dx.doi.org/10.1016/j.enzmictec.2018.06.001.
    10.1016/j.enzmictec.2018.06.001
  26. Fantoni, A.; Fernandes, M.; Vygranenko, Y.; Louro, P.; Vieira, M.; Silva, R. P. O.; Texeira, D.; et al. "Analysis of metallic nanoparticles embedded in thin film semiconductors for optoelectronic applications". Optical and Quantum Electronics 50 6 (2018): http://dx.doi.org/10.1007/s11082-018-1523-z.
    10.1007/s11082-018-1523-z
  27. Paulo, Pedro M. R.; Botequim, David; Jóskowiak, Agnieszka; Martins, Sofia; Prazeres, Duarte M. F.; Zijlstra, Peter; Costa, Sílvia M. B.. "Enhanced Fluorescence of a Dye on DNA-Assembled Gold Nanodimers Discriminated by Lifetime Correlation Spectroscopy". The Journal of Physical Chemistry C 122 20 (2018): 10971-10980. http://dx.doi.org/10.1021/acs.jpcc.7b12622.
    10.1021/acs.jpcc.7b12622
  28. Prazeres, Duarte. "Production and Purification of Supercoiled Minicircles by a Combination of In Vitro Endonuclease Nicking and Hydrophobic Interaction Chromatography". Hum. Gene Ther. Meth. (2018):
    10.1089/hgtb.2018.046
  29. Prazeres, D.M.F.; Matias, I.A.S.; Ribeiro, A.P.C.; Oliveira-Silva, R.P.; Martins, L.M.D.R.S.. "Gold Nanotriangles as Efficient Catalysts for the Selective Oxidation of Cyclohexane". App. Sci. 8 (2018): 2655.
    Published • 10.3390/app8122655
  30. Almeida, A.; Rosa, A.M.M.; Azevedo, A.M.; Prazeres, D.M.F.. "A biomolecular recognition approach for the functionalization of cellulose with gold nanoparticles". Journal of Molecular Recognition 30 9 (2017): e2634. http://dx.doi.org/10.1002/jmr.2634.
    10.1002/jmr.2634
  31. Prazeres, Duarte; Pires, Virgínia M. R.; Pereira, Pedro M. M.; Brás, Joana L. A.; Correia, Márcia; Cardoso, Vânia; Bule, Pedro; et al. "Stability and ligand promiscuity of type A carbohydrate-binding modules are illustrated by the structure of Spirochaeta thermophila StCBM64C". The Journal of Biological Chemistry 292 12 (2017): 4847-4860. http://www.jbc.org/content/early/2017/02/08/jbc.M116.767541.abstract.
    jbc.M116.767541
  32. Prazeres, Duarte; Rosa, A. M. M.; Prazeres, D. M. F.; Paulo, P. M. R.. "Fluorescence correlation spectroscopy study of the complexation of DNA hybrids, IgG antibody, and a chimeric protein of IgG-binding ZZ domains fused with a carbohydrate binding module". Phys. Chem. Chem. Phys. 19 25 (2017): 16606-16614. http://dx.doi.org/10.1039/C7CP00662D.
    10.1039/C7CP00662D
  33. Prazeres, Duarte. "Colorimetric detection of D-dimer in a paper-based immunodetection device". Analytical Biochemistry (2017): http://www.sciencedirect.com/science/article/pii/S0003269717303755.
    https://doi.org/10.1016/j.ab.2017.09.009
  34. Prazeres, Duarte. "A process for supercoiled plasmid DNA purification based on multimodal chromatography". Separation and Purification Technology 182 Supplement (2017): 94-100. http://www.sciencedirect.com/science/article/pii/S1383586616322420.
    https://doi.org/10.1016/j.seppur.2017.03.042
  35. Šimcíková, Michaela; Alves, Cláudia P. A.; Brito, Liliana; Prather, Kristala L. J.; Prazeres, Duarte M. F.; Monteiro, Gabriel A.. "Improvement of DNA minicircle production by optimization of the secondary structure of the 5'-UTR of ParA resolvase". Applied Microbiology and Biotechnology 100 15 (2016): 6725-6737. http://dx.doi.org/10.1007/s00253-016-7565-x.
    10.1007/s00253-016-7565-x
  36. Alves, Cláudia P.A.; Šimcíková, Michaela; Brito, Liliana; Monteiro, Gabriel A.; Prazeres, Duarte Miguel F.. "Development of a nicking endonuclease-assisted method for the purification of minicircles". Journal of Chromatography A 1443 (2016): 136-144. http://dx.doi.org/10.1016/j.chroma.2016.03.035.
    10.1016/j.chroma.2016.03.035
  37. Prazeres, Duarte. "Use of DNA Stabilizers to Extend Plasmid Biological Activity". Current Bionanotechnology (2016): http://benthamscience.com/journals/current-bionanotechnology/article/137823/.
    10.2174/2213529402666151214222447
  38. Prazeres, Duarte. "Separation of plasmid DNA topoisomers by multimodal chromatography". Analytical Biochemistry 503 (2016): 68-70. http://www.sciencedirect.com/science/article/pii/S0003269716001123.
    http://dx.doi.org/10.1016/j.ab.2016.03.012
  39. Prazeres, Duarte; Moita, Ana Sofia; Laurência, Catarina; Ramos, Joana Anastácio; Prazeres, Duarte Miguel França; Moreira, António Luís Nobre. "Dynamics of Droplets of Biological Fluids on Smooth Superhydrophobic Surfaces under Electrostatic Actuation". Journal of Bionic Engineering 13 2 (2016): 220-234. http://www.sciencedirect.com/science/article/pii/S1672652916602962.
    10.1016/S1672-6529(16)60296-2
  40. Prazeres, Duarte. "In situNIR spectroscopy monitoring of plasmid production processes: effect of producing strain, medium composition and the cultivation strategy". Journal of Chemical Technology & Biotechnology 90 2 (2015): 255-261. http://dx.doi.org/10.1002/jctb.4431.
    10.1002/jctb.4431
  41. Prazeres, Duarte. "Towards effective non-viral gene delivery vector". Biotechnology and Genetic Engineering Reviews 31 1-2 (2015): 82-107. http://dx.doi.org/10.1080/02648725.2016.1178011.
    10.1080/02648725.2016.1178011
  42. Prazeres, Duarte. "Preparation of liposome membrane adsorbers and testing for plasmid purification". Biochemical Engineering Journal 93 (2015): 1-10. http://www.sciencedirect.com/science/article/pii/S1369703X14002447.
    10.1016/j.bej.2014.09.005
  43. Martins, S.A.M.; Moulas, G.; Trabuco, J.R.C.; Monteiro, G.A.; Chu, V.; Conde, J.P.; Prazeres, D.M.F.. "Monitoring intracellular calcium in response to GPCR activation using thin-film silicon photodiodes with integrated fluorescence filters". Biosensors and Bioelectronics 52 (2014): 232-238. http://www.scopus.com/inward/record.url?eid=2-s2.0-84884386241&partnerID=MN8TOARS.
    10.1016/j.bios.2013.08.037
  44. Rosa, A.M.M.; Louro, A.F.; Martins, S.A.M.; Inácio, J.; Azevedo, A.M.; Prazeres, D.M.F.. "Capture and detection of DNA hybrids on paper via the anchoring of antibodies with fusions of carbohydrate binding modules and ZZ-domains". Analytical Chemistry 86 9 (2014): 4340-4347. http://www.scopus.com/inward/record.url?eid=2-s2.0-84899814597&partnerID=MN8TOARS.
    10.1021/ac5001288
  45. Simcikova, M.; Prather, K.L.J.; Prazeres, D.M.F.; Monteiro, G.A.. "On the dual effect of glucose during production of pBAD/AraC-based minicircles". Vaccine 32 24 (2014): 2843-2846. http://www.scopus.com/inward/record.url?eid=2-s2.0-84899953112&partnerID=MN8TOARS.
    10.1016/j.vaccine.2014.02.035
  46. Pereira, P.M.M.; Moita, A.S.; Monteiro, G.A.; Prazeres, D.M.F.. "Characterization of the topography and wettability of english weed leaves and biomimetic replicas". Journal of Bionic Engineering 11 3 (2014): 346-359. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904035178&partnerID=MN8TOARS.
    10.1016/S1672-6529(14)60048-2
  47. Raiado-Pereira, L.; Vega, J.D.L.; Prazeres, D.M.F.; Mateus, M.. "Development of a phenyl membrane chromatography-based process yielding pharmaceutical grade plasmid deoxyribonucleic acid for mammalian cells transfection". Journal of Chromatography A 1337 (2014): 67-74. http://www.scopus.com/inward/record.url?eid=2-s2.0-84900661426&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.02.024
  48. Gonçalves, G.A.L.; Prather, K.L.J.; Monteiro, G.A.; Carnes, A.E.; Prazeres, D.M.F.. "Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: Fermentation strategies and impact on downstream processing". Journal of Biotechnology 186 (2014): 119-127. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904721409&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2014.06.008
  49. Gonçalves, G.A.L.; Prather, K.L.J.; Monteiro, G.A.; Prazeres, D.M.F.. "Engineering of Escherichia coli strains for plasmid biopharmaceutical production: Scale-up challenges". Vaccine 32 24 (2014): 2847-2850. http://www.scopus.com/inward/record.url?eid=2-s2.0-84899980712&partnerID=MN8TOARS.
    10.1016/j.vaccine.2014.02.023
  50. Gonçalves, G.A.L.; Oliveira, P.H.; Gomes, A.G.; Prather, K.L.J.; Lewis, L.A.; Prazeres, D.M.F.; Monteiro, G.A.. "Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters". Applied Microbiology and Biotechnology 98 15 (2014): 6609-6619. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905023433&partnerID=MN8TOARS.
    10.1007/s00253-014-5695-6
  51. Prazeres, Duarte. "Plasmid Biopharmaceuticals". Microbiology Spectrum 2 6 (2014): http://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.PLAS-0022-2014.
    10.1128/microbiolspec.PLAS-0022-2014
  52. Novo, P.; Moulas, G.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Detection of ochratoxin A in wine and beer by chemiluminescence-based ELISA in microfluidics with integrated photodiodes". Sensors and Actuators, B: Chemical 176 (2013): 232-240. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875417047&partnerID=MN8TOARS.
    10.1016/j.snb.2012.10.038
  53. Donato, S.S.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Metabolic viability of Escherichia coli trapped by dielectrophoresis in microfluidics". Electrophoresis 34 4 (2013): 575-582. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873999112&partnerID=MN8TOARS.
    10.1002/elps.201200292
  54. Gonçalves, G.A.L.; Prazeres, D.M.F.; Monteiro, G.A.; Prather, K.L.J.. "De novo creation of MG1655-derived E. coli strains specifically designed for plasmid DNA production". Applied Microbiology and Biotechnology 97 2 (2013): 611-620. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873993482&partnerID=MN8TOARS.
    10.1007/s00253-012-4308-5
  55. Raiado-Pereira, L.; Prazeres, D.M.F.; Mateus, M.. "Impact of plasmid size on the purification of model plasmid DNA vaccines by phenyl membrane adsorbers". Journal of Chromatography A 1315 (2013): 145-151. http://www.scopus.com/inward/record.url?eid=2-s2.0-84885371617&partnerID=MN8TOARS.
    10.1016/j.chroma.2013.09.076
  56. La Vega, J.D.; Braak, B.T.; Azzoni, A.R.; Monteiro, G.A.; Prazeres, D.M.F.. "Impact of plasmid quality on lipoplex-mediated transfection". Journal of Pharmaceutical Sciences 102 11 (2013): 3932-3941. http://www.scopus.com/inward/record.url?eid=2-s2.0-84885842863&partnerID=MN8TOARS.
    10.1002/jps.23709
  57. Martins, D.C.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Streaming currents in microfluidics with integrated polarizable electrodes". Microfluidics and Nanofluidics 15 3 (2013): 361-376. http://www.scopus.com/inward/record.url?eid=2-s2.0-84883264442&partnerID=MN8TOARS.
    10.1007/s10404-013-1153-5
  58. Joskowiak, A.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Amorphous silicon photosensors for detection of intrinsic cell fluorophores". Materials Research Society Symposium Proceedings 1321 (2012): 435-439. http://www.scopus.com/inward/record.url?eid=2-s2.0-84455190921&partnerID=MN8TOARS.
    10.1557/opl.2011.1230
  59. Martins, D.C.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Streaming current measurements in micro and nanofluidic channels for label-free multiplexed genomics diagnostics". Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (2012): 886-889. http://www.scopus.com/inward/record.url?eid=2-s2.0-84860486822&partnerID=MN8TOARS.
    10.1109/MEMSYS.2012.6170328
  60. Martins, S.M.A.; Trabuco, J.R.G.; Monteiro, G.A.; Prazeres, D.M.. "GPCR screening and drug discovery: Challenges and latest trends". European Pharmaceutical Review 17 2 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84876329052&partnerID=MN8TOARS.
  61. Gomes, A.G.; Azevedo, A.M.; Aires-Barros, M.R.; Prazeres, D.M.F.. "Validation and scale-up of plasmid DNA purification by phenyl-boronic acid chromatography". Journal of Separation Science 35 22 (2012): 3190-3196. http://www.scopus.com/inward/record.url?eid=2-s2.0-84870021495&partnerID=MN8TOARS.
    10.1002/jssc.201200225
  62. Jóskowiak, A.; Stasio, N.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Integrated detection of intrinsic fluorophores in live microbial cells using an array of thin film amorphous silicon photodetectors". Biosensors and Bioelectronics 36 1 (2012): 242-249. http://www.scopus.com/inward/record.url?eid=2-s2.0-84861715094&partnerID=MN8TOARS.
    10.1016/j.bios.2012.04.029
  63. Gonçalves, G.A.L.; Bower, D.M.; Prazeres, D.M.F.; Monteiro, G.A.; Prather, K.L.J.. "Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing". Biotechnology Journal 7 2 (2012): 251-261. http://www.scopus.com/inward/record.url?eid=2-s2.0-84856575274&partnerID=MN8TOARS.
    10.1002/biot.201100062
  64. Lewis, L.A.; Astatke, M.; Umekubo, P.T.; Alvi, S.; Saby, R.; Afrose, J.; Oliveira, P.H.; Monteiro, G.A.; Prazeres, D.M.F.. "Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition". Mobile DNA 3 1 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84860627601&partnerID=MN8TOARS.
    10.1186/1759-8753-3-1
  65. Martins, Sofia A. M.. "Towards the miniaturization of GPCR-based live-cell screening assays". Trends in Biotechnology 30 11 (2012): 566-574.
    10.1016/j.tibtech.2012.07.004
  66. Toledo, M.A.S.; Janissen, R.; Favaro, M.T.P.; Cotta, M.A.; Monteiro, G.A.; Prazeres, D.M.F.; Souza, A.P.; Azzoni, A.R.. "Development of a recombinant fusion protein based on the dynein light chain LC8 for non-viral gene delivery". Journal of Controlled Release 159 2 (2012): 222-231. http://www.scopus.com/inward/record.url?eid=2-s2.0-84861095871&partnerID=MN8TOARS.
    10.1016/j.jconrel.2012.01.011
  67. Martins, S.A.M.; Moulas, G.; Trabuco, J.R.C.; Monteiro, G.A.; Chu, V.; Conde, J.P.; Prazeres, D.M.F.. "Integrated On-chip Photodetection of Intracellular Calcium in Response to the Activation of G-protein Coupled Receptors". Procedia Engineering 47 (2012): 993-996. http://dx.doi.org/10.1016/j.proeng.2012.09.314.
    Published • 10.1016/j.proeng.2012.09.314
  68. Monteiro, M.E.; Raiado–Pereira, L.; Prazeres, D.M.F.; Mateus, M.. "Liposome Derived Membrane Adsorber for Purification of Nucleic Acids". Procedia Engineering 44 (2012): 1463-1464. http://dx.doi.org/10.1016/j.proeng.2012.08.829.
    Published • 10.1016/j.proeng.2012.08.829
  69. Gomes, A.G.; Azevedo, A.M.; Aires-Barros, M.R.; Prazeres, D.M.F.. "Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads". Journal of Chromatography A 1218 48 (2011): 8629-8637. http://www.scopus.com/inward/record.url?eid=2-s2.0-80455131119&partnerID=MN8TOARS.
    10.1016/j.chroma.2011.10.004
  70. Lança, A.S.C.; de Sousa, K.P.; Atouguia, J.; Prazeres, D.M.F.; Monteiro, G.A.; Silva, M.S.. "Trypanosoma brucei: Immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis". Experimental Parasitology 127 1 (2011): 18-24. http://www.scopus.com/inward/record.url?eid=2-s2.0-78650529440&partnerID=MN8TOARS.
    10.1016/j.exppara.2010.06.017
  71. Jóskowiak, A.; Santos, M.S.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Integration of thin film amorphous silicon photodetector with lab-on-chip for monitoring protein fluorescence in solution and in live microbial cells". Sensors and Actuators, B: Chemical 156 2 (2011): 662-667. http://www.scopus.com/inward/record.url?eid=2-s2.0-79958094386&partnerID=MN8TOARS.
    10.1016/j.snb.2011.02.015
  72. Pereira, A.T.; Novo, P.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Heterogeneous immunoassays in microfluidic format using fluorescence detection with integrated amorphous silicon photodiodes". Biomicrofluidics 5 1 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-79953286045&partnerID=MN8TOARS.
    10.1063/1.3553014
  73. Novo, P.; Frana Prazeres, D.M.; Chu, V.; Conde, J.P.. "Microspot-based ELISA in microfluidics: Chemiluminescence and colorimetry detection using integrated thin-film hydrogenated amorphous silicon photodiodes". Lab on a Chip - Miniaturisation for Chemistry and Biology 11 23 (2011): 4063-4071. http://www.scopus.com/inward/record.url?eid=2-s2.0-80755125455&partnerID=MN8TOARS.
    10.1039/c1lc20362b
  74. Novo, P.; Moulas, G.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Lab-on-a-chip ochratoxin A detection using competitive ELISA in microfluidics with integrated photodiode signal acquisition". Procedia Engineering 25 (2011): 1205-1208. http://www.scopus.com/inward/record.url?eid=2-s2.0-84857181083&partnerID=MN8TOARS.
    Published • 10.1016/j.proeng.2011.12.297
  75. Prazeres, D.M.F.; Martins, S.A.M.; Trabuco, J.R.C.; Monteiro, G.A.; Juskowiak, A.; Conde, J.P.; Chu, V.. "Towards a high-throughput drug discovery platform for the screening of GPCR targets in cells". 1st Portuguese Meeting in Biomedical Engineering, ENBENG 2011 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-80053931999&partnerID=MN8TOARS.
    10.1109/ENBENG.2011.6026042
  76. Prazeres, D.M.F.. "Plasmid Biopharmaceuticals: Basics, Applications, and Manufacturing". Plasmid Biopharmaceuticals: Basics, Applications, and Manufacturing (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-80455143687&partnerID=MN8TOARS.
    10.1002/9780470939918
  77. Oliveira, P.H.; Prather, K.L.J.; Prazeres, D.M.F.; Monteiro, G.A.. "Mutation detection in plasmid-based biopharmaceuticals". Biotechnology Journal 6 4 (2011): 378-391. http://www.scopus.com/inward/record.url?eid=2-s2.0-79953676001&partnerID=MN8TOARS.
    10.1002/biot.201000372
  78. Guerrero-Germán, P.; Montesinos-Cisneros, R.Ma.; Prazeres, D.M.F.; Tejeda-Mansir, A.. "Purification of plasmid DNA from Escherichia coli ferments using anion-exchange membrane and hydrophobic chromatography". Biotechnology and Applied Biochemistry 58 1 (2011): 68-74. http://www.scopus.com/inward/record.url?eid=2-s2.0-79953245624&partnerID=MN8TOARS.
    10.1002/bab.12
  79. Martins, D.C.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Electrical detection of DNA immobilization and hybridization by streaming current measurements in microchannels". Applied Physics Letters 99 18 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-80855137016&partnerID=MN8TOARS.
    10.1063/1.3658457
  80. Conde, J.P.; Jóskowiak, A.; Lipovšek, B.; Pimentel, A.; Pereira, A.T.; Santos, M.; Krc, J.; et al. "Spectral selectivity constraints in fluorescence detection of biomolecules using amorphous silicon based detectors". Physica Status Solidi (C) Current Topics in Solid State Physics 7 3-4 (2010): 1156-1159. http://www.scopus.com/inward/record.url?eid=2-s2.0-77952571943&partnerID=MN8TOARS.
    10.1002/pssc.200982771
  81. Pereira, L.R.; Prazeres, D.M.F.; Mateus, M.. "Hydrophobic interaction membrane chromatography for plasmid DNA purification: Design and optimization". Journal of Separation Science 33 9 (2010): 1175-1184. http://www.scopus.com/inward/record.url?eid=2-s2.0-77952206845&partnerID=MN8TOARS.
    10.1002/jssc.200900844
  82. Cabeça, R.; Martins, V.; Ferraria, A.M.; Do Rego, A.M.B.; Brogueira, P.; Prazeres, D.M.F.; Fonseca, L.P.; Chu, V.; Conde, J.P.. "Patterned functionalization layer for sub-µL DNA solid-phase immobilization and hybridization". Sensors and Actuators, B: Chemical 149 2 (2010): 432-438. http://www.scopus.com/inward/record.url?eid=2-s2.0-77955425081&partnerID=MN8TOARS.
    10.1016/j.snb.2010.04.019
  83. Martins, S.A.M.; Prazeres, D.M.F.; Fonseca, L.P.; Monteiro, G.A.. "Optimization of DNA hybridization on aminopropyl-controlled pore-glass particles: Detection of non-labeled targets by picogreen staining". Analytical Letters 43 17 (2010): 2694-2704. http://www.scopus.com/inward/record.url?eid=2-s2.0-78649760138&partnerID=MN8TOARS.
    10.1080/00032711003731431
  84. Martins, S.A.M.; Prazeres, D.M.F.; Fonseca, L.P.; Monteiro, G.A.. "Quantitation of non-amplified genomic DNA by bead-based hybridization and template mediated extension coupled to alkaline phosphatase signal amplification". Biotechnology Letters 32 2 (2010): 229-234. http://www.scopus.com/inward/record.url?eid=2-s2.0-76449110832&partnerID=MN8TOARS.
    10.1007/s10529-009-0149-4
  85. Oliveira, Pedro H.. "Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events". Applied Microbiology and Biotechnology 87 6 (2010): 2157-2167.
    10.1007/s00253-010-2671-7
  86. Azevedo, A.M.; Gomes, A.G.; Borlido, L.; Santos, I.F.S.; Prazeres, D.M.F.; Aires-Barros, M.R.. "Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography". Journal of Molecular Recognition 23 6 (2010): 569-576. http://www.scopus.com/inward/record.url?eid=2-s2.0-78049449358&partnerID=MN8TOARS.
    10.1002/jmr.1068
  87. Lipovek, B.; Jóskowiak, A.; Krc, J.; Topic, M.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Characterisation of hydrogenated silicon-carbon alloy filters with different carbon composition for on-chip fluorescence detection of biomolecules". Sensors and Actuators, A: Physical 163 1 (2010): 96-100. http://www.scopus.com/inward/record.url?eid=2-s2.0-77957673064&partnerID=MN8TOARS.
    10.1016/j.sna.2010.07.016
  88. Carvalho, J.A.; Rodgers, J.; Atouguia, J.; Prazeres, D.M.; Monteiro, G.A.. "DNA vaccines: A rational design against parasitic diseases". Expert Review of Vaccines 9 2 (2010): 175-191. http://www.scopus.com/inward/record.url?eid=2-s2.0-77950353912&partnerID=MN8TOARS.
    10.1586/erv.09.158
  89. Carvalho, J.A.; Azzoni, A.R.; Prazeres, D.M.F.; Monteiro, G.A.. "Comparative analysis of antigen-targeting sequences used in DNA vaccines". Molecular Biotechnology 44 3 (2010): 204-212. http://www.scopus.com/inward/record.url?eid=2-s2.0-77649237006&partnerID=MN8TOARS.
    10.1007/s12033-009-9229-x
  90. Gomes, A.G.; Azevedo, A.M.; Aires-Barros, M.R.; Prazeres, D.M.F.. "Clearance of host cell impurities from plasmid-containing lysates by boronate adsorption". Journal of Chromatography A 1217 15 (2010): 2262-2266. http://www.scopus.com/inward/record.url?eid=2-s2.0-77950690475&partnerID=MN8TOARS.
    10.1016/j.chroma.2010.02.015
  91. Ribeiro, S.C.; Prazeres, D.M.F.; Monteiro, G.A.. "Evidence for the in vivo expression of a distant downstream gene under the control of ColE1 replication origin". Applied Microbiology and Biotechnology 86 2 (2010): 671-679. http://www.scopus.com/inward/record.url?eid=2-s2.0-77249130203&partnerID=MN8TOARS.
    10.1007/s00253-009-2339-3
  92. Freitas, S.; Canário, S.; Santos, J.A.L.; Prazeres, D.M.F.. "Alternatives for the intermediate recovery of plasmid DNA: Performance, economic viability and environmental impact". Biotechnology Journal 4 2 (2009): 265-278. http://www.scopus.com/inward/record.url?eid=2-s2.0-62549115807&partnerID=MN8TOARS.
    10.1002/biot.200800216
  93. Oliveira, P.H.; Prather, K.J.; Prazeres, D.M.F.; Monteiro, G.A.. "Structural instability of plasmid biopharmaceuticals: challenges and implications". Trends in Biotechnology 27 9 (2009): 503-511. http://www.scopus.com/inward/record.url?eid=2-s2.0-68749104564&partnerID=MN8TOARS.
    10.1016/j.tibtech.2009.06.004
  94. Wu, M.L.; Freitas, S.S.; Monteiro, G.A.; Prazeres, D.M.F.; Santos, J.A.L.. "Stabilization of naked and condensed plasmid DNA against degradation induced by ultrasounds and high-shear vortices". Biotechnology and Applied Biochemistry 53 4 (2009): 237-246. http://www.scopus.com/inward/record.url?eid=2-s2.0-67749102274&partnerID=MN8TOARS.
    10.1042/BA20080215
  95. Pinotti, L.M.; Fonseca, L.P.; Prazeres, D.M.F.; Rodrigues, D.S.; Nucci, E.R.; Giordano, R.L.C.. "Recovery and partial purification of penicillin G acylase from E. coli homogenate and B. megaterium culture medium using an expanded bed adsorption column". Biochemical Engineering Journal 44 2-3 (2009): 111-118. http://www.scopus.com/inward/record.url?eid=2-s2.0-61449267560&partnerID=MN8TOARS.
    10.1016/j.bej.2008.11.006
  96. Silva, M.S.; Prazeres, D.M.F.; Lança, A.; Atouguia, J.; Monteiro, G.A.. "Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis". Parasitology Research 105 5 (2009): 1223-1229. http://www.scopus.com/inward/record.url?eid=2-s2.0-70349593708&partnerID=MN8TOARS.
    10.1007/s00436-009-1542-6
  97. Martins, S.A.M.; Prazeres, D.M.F.; Fonseca, L.P.; Monteiro, G.A.. "The role of probe-probe interactions on the hybridization of double-stranded DNA targets onto DNA-modified magnetic microparticles". Analytical and Bioanalytical Chemistry 394 6 (2009): 1711-1716. http://www.scopus.com/inward/record.url?eid=2-s2.0-67651005151&partnerID=MN8TOARS.
    10.1007/s00216-009-2843-4
  98. Cabeça, R.; Rodrigues, M.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "The effect of the shape of single, sub-ms voltage pulses on the rates of surface immobilization and hybridization of DNA". Nanotechnology 20 1 (2009): http://www.scopus.com/inward/record.url?eid=2-s2.0-58149265153&partnerID=MN8TOARS.
    10.1088/0957-4484/20/1/015503
  99. Martins, D.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Ionic Conductivity Measurements in a SiO 2 Nanochannel with PDMS Interconnects". Procedia Chemistry 1 1 (2009): 1095-1098. http://www.scopus.com/inward/record.url?eid=2-s2.0-71549127103&partnerID=MN8TOARS.
    10.1016/j.proche.2009.07.273
  100. Sousa, F.; Prazeres, D.M.F.; Queiroz, J.A.. "Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography". Journal of Gene Medicine 11 1 (2009): 79-88. http://www.scopus.com/inward/record.url?eid=2-s2.0-59449090459&partnerID=MN8TOARS.
    10.1002/jgm.1272
  101. Sousa, A.; Sousa, F.; Prazeres, D.M.F.; Queiroz, J.A.. "Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention". Biomedical Chromatography 23 7 (2009): 745-753. http://www.scopus.com/inward/record.url?eid=2-s2.0-69749128313&partnerID=MN8TOARS.
    10.1002/bmc.1179
  102. Pereira, A.T.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Miniaturization of immunoassays using optical detection with integrated amorphous silicon photodiodes". Materials Research Society Symposium Proceedings 1191 (2009): 73-78. http://www.scopus.com/inward/record.url?eid=2-s2.0-77649086845&partnerID=MN8TOARS.
  103. Pereira, A.T.; Pimentel, A.C.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Chemiluminescent detection of horseradish peroxidase using an integrated amorphous silicon thin-film photosensor". IEEE Sensors Journal 9 10 (2009): 1282-1290. http://www.scopus.com/inward/record.url?eid=2-s2.0-78349249753&partnerID=MN8TOARS.
    10.1109/JSEN.2009.2027414
  104. Freitas, S.S.; Santos, J.A.L.; Prazeres, D.M.F.. "Plasmid purification by hydrophobic interaction chromatography using sodium citrate in the mobile phase". Separation and Purification Technology 65 1 (2009): 95-104. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149295441&partnerID=MN8TOARS.
    10.1016/j.seppur.2008.04.001
  105. Gomes, G.A.; Azevedo, A.M.; Aires-Barros, M.R.; Prazeres, D.M.F.. "Purification of plasmid DNA with aqueous two phase systems of PEG 600 and sodium citrate/ammonium sulfate". Separation and Purification Technology 65 1 (2009): 22-30. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149289804&partnerID=MN8TOARS.
    10.1016/j.seppur.2008.01.026
  106. Guerrero-Germán, P.; Prazeres, D.M.F.; Guzmán, R.; Montesinos-Cisneros, R.M.; Tejeda-Mansir, A.. "Purification of plasmid DNA using tangential flow filtration and tandem anion-exchange membrane chromatography". Bioprocess and Biosystems Engineering 32 5 (2009): 615-623. http://www.scopus.com/inward/record.url?eid=2-s2.0-68349139498&partnerID=MN8TOARS.
    10.1007/s00449-008-0284-7
  107. Carvalho, J.A.; Prazeres, D.M.; Monteiro, G.A.. "Bringing DNA vaccines closer to commercial use". IDrugs 12 10 (2009): 642-647. http://www.scopus.com/inward/record.url?eid=2-s2.0-70349759753&partnerID=MN8TOARS.
  108. Sousa, F.; Prazeres, D.M.F.; Queiroz, J.A.. "Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification". Biomedical Chromatography 23 2 (2009): 160-165. http://www.scopus.com/inward/record.url?eid=2-s2.0-61349098946&partnerID=MN8TOARS.
    10.1002/bmc.1097
  109. Martins, S.A.M.; Prazeres, D.M.F.; Fonseca, L.P.; Monteiro, G.A.. "Application of central composite design for DNA hybridization onto magnetic microparticles". Analytical Biochemistry 391 1 (2009): 17-23. http://www.scopus.com/inward/record.url?eid=2-s2.0-67349094138&partnerID=MN8TOARS.
    10.1016/j.ab.2009.05.006
  110. Pimentel, A.C.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Comparison of amorphous silicon photodiodes and photoconductors for detection of quantum dot biomolecular tags". Journal of Applied Physics 106 10 (2009): http://www.scopus.com/inward/record.url?eid=2-s2.0-71749088951&partnerID=MN8TOARS.
    10.1063/1.3259389
  111. Henriques, A.M.; Madeira, C.; Fevereiro, M.; Prazeres, D.M.F.; Aires-Barros, M.R.; Monteiro, G.A.. "Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus". International Journal of Pharmaceutics 377 1-2 (2009): 92-98. http://www.scopus.com/inward/record.url?eid=2-s2.0-67649658026&partnerID=MN8TOARS.
    10.1016/j.ijpharm.2009.05.005
  112. Pimentel, A.C.; Pereira, A.T.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Detection of fluorescently labeled biomolecules immobilized on a detachable substrate using an integrated amorphous silicon photodetector". Applied Physics Letters 94 16 (2009): http://www.scopus.com/inward/record.url?eid=2-s2.0-65449166779&partnerID=MN8TOARS.
    10.1063/1.3122345
  113. Oliveira, P.H.; Prazeres, D.M.F.; Monteiro, G.A.. "Deletion formation mutations in plasmid expression vectors are unfavored by runaway amplification conditions and differentially selected under kanamycin stress". Journal of Biotechnology 143 4 (2009): 231-238. http://www.scopus.com/inward/record.url?eid=2-s2.0-70249151468&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2009.08.002
  114. Ribeiro, S.C.; Monteiro, G.A.; Prazeres, D.M.F.. "Evaluation of the effect of Non-B DNA structures on plasmid integrity via accelerated stability studies". Journal of Pharmaceutical Sciences 98 4 (2009): 1400-1408. http://www.scopus.com/inward/record.url?eid=2-s2.0-67449152210&partnerID=MN8TOARS.
    10.1002/jps.21503
  115. Pereira, A.T.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Enzymatic biosensors with integrated thin film a-Si:H photodiodes". Materials Research Society Symposium Proceedings 1153 (2009): 179-184. http://www.scopus.com/inward/record.url?eid=2-s2.0-77951108882&partnerID=MN8TOARS.
  116. Gonçlves, D.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Amorphous silicon thin-film transistors gated through an electrolyte solution". IEEE Electron Device Letters 29 9 (2008): 1030-1033. http://www.scopus.com/inward/record.url?eid=2-s2.0-50649118865&partnerID=MN8TOARS.
    10.1109/LED.2008.2001406
  117. Sousa, F.; Matos, T.; Prazeres, D.M.F.; Queiroz, J.A.. "Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography". Analytical Biochemistry 374 2 (2008): 432-434. http://www.scopus.com/inward/record.url?eid=2-s2.0-39149129979&partnerID=MN8TOARS.
    10.1016/j.ab.2007.11.005
  118. Ribeiro, S.C.; Oliveira, P.H.; Prazeres, D.M.F.; Monteiro, G.A.. "High frequency plasmid recombination mediated by 28 bp direct repeats". Molecular Biotechnology 40 3 (2008): 252-260. http://www.scopus.com/inward/record.url?eid=2-s2.0-62349140161&partnerID=MN8TOARS.
    10.1007/s12033-008-9082-3
  119. Prazeres, D.M.F.. "Prediction of diffusion coefficients of plasmids". Biotechnology and Bioengineering 99 4 (2008): 1040-1044. http://www.scopus.com/inward/record.url?eid=2-s2.0-39549086017&partnerID=MN8TOARS.
    10.1002/bit.21626
  120. Sousa, F.; Prazeres, D.M.F.; Queiroz, J.A.. "Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA". Trends in Biotechnology 26 9 (2008): 518-525. http://www.scopus.com/inward/record.url?eid=2-s2.0-48849111296&partnerID=MN8TOARS.
    10.1016/j.tibtech.2008.05.005
  121. Oliveira, P.H.; Lemos, F.; Monteiro, G.A.; Prazeres, D.M.F.. "Recombination frequency in plasmid DNA containing direct repeats-predictive correlation with repeat and intervening sequence length". Plasmid 60 2 (2008): 159-165. http://www.scopus.com/inward/record.url?eid=2-s2.0-49949118846&partnerID=MN8TOARS.
    10.1016/j.plasmid.2008.06.004
  122. Martins, S.A.M.; Prazeres, D.M.F.; Fonseca, L.P.; Monteiro, G.A.. "Chemiluminescent bead-based hybridization assay for the detection of genomic DNA from E. coli in purified plasmid samples". Analytical and Bioanalytical Chemistry 391 6 (2008): 2179-2187. http://www.scopus.com/inward/record.url?eid=2-s2.0-46249104300&partnerID=MN8TOARS.
    10.1007/s00216-008-2142-5
  123. Gouvêa, A.; Pereira, A.T.; Pimentel, A.C.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Colorimetric detection of molecular recognition reactions with an enzyme biolabel using a thin-film amorphous silicon photodiode on a glass substrate". Sensors and Actuators, B: Chemical 135 1 (2008): 102-107. http://www.scopus.com/inward/record.url?eid=2-s2.0-56049106336&partnerID=MN8TOARS.
    10.1016/j.snb.2008.07.030
  124. Cabeca, R.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Electric field assisted DNA surface reactions on the sub-ms timescale". Materials Research Society Symposium Proceedings 1092 (2008): 1-7. http://www.scopus.com/inward/record.url?eid=2-s2.0-70350316207&partnerID=MN8TOARS.
  125. Conde, J.P.; Pimentel, A.C.; Pereira, A.T.; Gouvêa, A.; Prazeres, D.M.F.; Chu, V.. "Detection of molecular tags with an integrated amorphous silicon photodetector for biological applications". Journal of Non-Crystalline Solids 354 19-25 (2008): 2594-2597. http://www.scopus.com/inward/record.url?eid=2-s2.0-42649125569&partnerID=MN8TOARS.
    10.1016/j.jnoncrysol.2007.09.096
  126. Gonçalves, D.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Detection of DNA and proteins using amorphous silicon ion-sensitive thin-film field effect transistors". Biosensors and Bioelectronics 24 4 (2008): 545-551. http://www.scopus.com/inward/record.url?eid=2-s2.0-53049083320&partnerID=MN8TOARS.
    10.1016/j.bios.2008.05.006
  127. Pimentel, A.C.; Cabeça, R.; Rodrigues, M.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Fluorescence detection of DNA hybridization using an integrated thin-film amorphous silicon n-i-p photodiode". Materials Research Society Symposium Proceedings 1066 (2008): 451-456. http://www.scopus.com/inward/record.url?eid=2-s2.0-62949118427&partnerID=MN8TOARS.
  128. Pimentel, A.C.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Fluorescence detection of DNA using an amorphous silicon p-i-n photodiode". Journal of Applied Physics 104 5 (2008): http://www.scopus.com/inward/record.url?eid=2-s2.0-51849097217&partnerID=MN8TOARS.
    10.1063/1.2976343
  129. Cabeça, R.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Electrical and chemical control of surfaces for DNA immobilization and hybridization". Materials Research Society Symposium Proceedings 1093 (2008): 19-26. http://www.scopus.com/inward/record.url?eid=2-s2.0-77957964936&partnerID=MN8TOARS.
  130. Pimentel, Alexandra C.. "Detection of chemiluminescence using an amorphous silicon photodiode". Ieee Sensors Journal 7 3-4 (2007): 415-416.
    10.1109/JSEN.2006.890144
  131. Carapuça, E.; Azzoni, A.R.; Prazeres, D.M.F.; Monteiro, G.A.; Mergulhão, F.J.M.. "Time-course determination of plasmid content in eukaryotic and prokaryotic cells using Real-Time PCR". Molecular Biotechnology 37 2 (2007): 120-126. http://www.scopus.com/inward/record.url?eid=2-s2.0-34248999538&partnerID=MN8TOARS.
    10.1007/s12033-007-0007-3
  132. Azzoni, A.R.; Ribeiro, S.C.; Monteiro, G.A.; Prazeres, D.M.F.. "The impact of polyadenylation signals on plasmid nuclease-resistance and transgene expression". Journal of Gene Medicine 9 5 (2007): 392-402. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249081884&partnerID=MN8TOARS.
    10.1002/jgm.1031
  133. Pistor, P.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "pH sensitive photoconductor based on poly(para-phenylene-vinylene)". Sensors and Actuators, B: Chemical 123 1 (2007): 153-157. http://www.scopus.com/inward/record.url?eid=2-s2.0-33947642058&partnerID=MN8TOARS.
    10.1016/j.snb.2006.08.005
  134. Pereira, A.T.; Pimentel, A.; Chu, V.; Prazeres, D.M.F.; Condel, J.P.. "On-chip detection of chemiluminescent biomolecules using an integrated thin film silicon photodiode". Materials Research Society Symposium Proceedings 1004 (2007): 113-118. http://www.scopus.com/inward/record.url?eid=2-s2.0-70349900923&partnerID=MN8TOARS.
  135. Freitas, S.S.; Azzoni, A.R.; Santos, J.A.L.; Monteiro, G.A.; Prazeres, D.M.F.. "On the stability of plasmid DNA vectors during cell culture and purification". Molecular Biotechnology 36 2 (2007): 151-158. http://www.scopus.com/inward/record.url?eid=2-s2.0-35848959238&partnerID=MN8TOARS.
    10.1007/s12033-007-0028-y
  136. Cabeça, R.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "On-chip control of DNA immobilization and hybridization with nanosecond electric field pulses". TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems (2007): 959-962. http://www.scopus.com/inward/record.url?eid=2-s2.0-50049121054&partnerID=MN8TOARS.
    10.1109/SENSOR.2007.4300291
  137. Duarte, S.P.; Fortes, A.G.; Prazeres, D.M.F.; Marcos, J.C.. "Preparation of plasmid DNA polyplexes from alkaline lysates by a two-step aqueous two-phase extraction process". Journal of Chromatography A 1164 1-2 (2007): 105-112. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548030710&partnerID=MN8TOARS.
    10.1016/j.chroma.2007.06.061
  138. Adrega, T.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Performance of thin-film a-Si:H microresonators in dissipative media". Materials Research Society Symposium Proceedings 910 (2007): 491-496. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249945102&partnerID=MN8TOARS.
  139. Teles, F.R.R.; Dos Prazeres, D.M.F.; De Lima-Filho, J.L.. "Electrochemical detection of a dengue-related oligonucleotide sequence using ferrocenium as a hybridization indicator". Sensors 7 11 (2007): 2510-2518. http://www.scopus.com/inward/record.url?eid=2-s2.0-36849009578&partnerID=MN8TOARS.
  140. Dias, A.I.; Feliciano, A.S.; Cabral, J.M.S.; Prazeres, D.M.F.. "Chemical synthesis and crystallization of the dipeptide AcPheIleNH2 in TTAB/heptane/octanol reversed micelles". Journal of Colloid and Interface Science 305 1 (2007): 198-201. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750958876&partnerID=MN8TOARS.
    10.1016/j.jcis.2006.09.052
  141. Sousa, F.; Prazeres, D.M.F.; Queiroz, J.A.. "Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography". Archives of Biochemistry and Biophysics 467 2 (2007): 154-162. http://www.scopus.com/inward/record.url?eid=2-s2.0-35748978569&partnerID=MN8TOARS.
    10.1016/j.abb.2007.08.027
  142. Sousa, F.; Prazeres, D.M.F.; Queiroz, J.A.. "Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography". Biomedical Chromatography 21 9 (2007): 993-998. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547966151&partnerID=MN8TOARS.
    10.1002/bmc.846
  143. Henriques, A.M.; Fevereiro, M.; Prazeres, D.M.F.; Monteiro, G.A.. "Development of a candidate DNA vaccine against Maedi-Visna virus". Veterinary Immunology and Immunopathology 119 3-4 (2007): 222-232. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548385665&partnerID=MN8TOARS.
    10.1016/j.vetimm.2007.05.004
  144. Moreira, K.A.; Chaves, A.C.; Marques, E.T.; Prazeres, D.M.F.; De Azevedo, W.M.; Porto, A.L.F.; De Filho, J.L.L.. "Extraction of dengue 2 plasmid DNA vaccine (pD2) from cell lysates by aqueous two-phase systems". Biotechnology 6 4 (2007): 520-526. http://www.scopus.com/inward/record.url?eid=2-s2.0-37249075900&partnerID=MN8TOARS.
  145. Wu, Ming Liang; Prazeres, Duarte; Monteiro, Gabriel; Santos, Jose. "Stabilization of plasmid DNA against mechanical damage". Journal of Biotechnology 131 2 (2007): S189-S189. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000249687100324&KeyUID=WOS:000249687100324.
    10.1016/j.jbiotec.2007.07.336
  146. Sousa, F.; Freitas, S.; Azzoni, A.R.; Prazeres, D.M.F.; Queiroz, J.. "Selective purification of supercoiled plasmid DNA from clarified cell lysates with a single histidine-agarose chromatography step". Biotechnology and Applied Biochemistry 45 3 (2006): 131-140. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750607427&partnerID=MN8TOARS.
    10.1042/BA20060082
  147. Adrega, T.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Thin-film silicon MEMS DNA sensors". Journal of Non-Crystalline Solids 352 9-20 SPEC. (2006): 1999-2003. http://www.scopus.com/inward/record.url?eid=2-s2.0-33744540993&partnerID=MN8TOARS.
    10.1016/j.jnoncrysol.2006.01.079
  148. Goncalves, D.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "a-Si:H electrolyte-gate thin film devices for biological applications". Materials Research Society Symposium Proceedings 926 (2006): 76-81. http://www.scopus.com/inward/record.url?eid=2-s2.0-33947710634&partnerID=MN8TOARS.
  149. Gonçalves, D.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Label-free electronic detection of biomolecules using a-Si:H field-effect devices". Journal of Non-Crystalline Solids 352 9-20 SPEC. (2006): 2007-2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-33744549938&partnerID=MN8TOARS.
    10.1016/j.jnoncrysol.2006.01.058
  150. Passarinha, L.A.; Diogo, M.M.; Queiroz, J.A.; Monteiro, G.A.; Fonseca, L.P.; Prazeres, D.M.F.. "Production of ColE1 type plasmid by Escherichia coli DH5a cultured under nonselective conditions". Journal of Microbiology and Biotechnology 16 1 (2006): 20-24. http://www.scopus.com/inward/record.url?eid=2-s2.0-32944454986&partnerID=MN8TOARS.
  151. Valente, C.A.; Monteiro, G.A.; Cabral, J.M.S.; Fevereiro, M.; Prazeres, D.M.F.. "Optimization of the primary recovery of human interferon a2b from Escherichia coli inclusion bodies". Protein Expression and Purification 45 1 (2006): 226-234. http://www.scopus.com/inward/record.url?eid=2-s2.0-28844455097&partnerID=MN8TOARS.
    10.1016/j.pep.2005.06.014
  152. Freitas, S.S.; Santos, J.A.L.; Prazeres, D.M.F.. "Optimization of isopropanol and ammonium sulfate precipitation steps in the purification of plasmid DNA". Biotechnology Progress 22 4 (2006): 1179-1186. http://www.scopus.com/inward/record.url?eid=2-s2.0-33747166436&partnerID=MN8TOARS.
    10.1021/bp060052d
  153. Sousa, F.; Tomaz, C.T.; Prazeres, D.M.F.; Queiroz, J.A.. "Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine-agarose support". Analytical Biochemistry 343 1 (2005): 183-185. http://www.scopus.com/inward/record.url?eid=2-s2.0-22144443546&partnerID=MN8TOARS.
    10.1016/j.ab.2005.03.011
  154. Fixe, F.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Single base mismatch detection by microsecond voltage pulses". Biosensors and Bioelectronics 21 6 (2005): 888-893. http://www.scopus.com/inward/record.url?eid=2-s2.0-27444445491&partnerID=MN8TOARS.
    10.1016/j.bios.2005.02.011
  155. Adrega, T.; Gaspar, J.; Fixe, F.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Thin film silicon microbridges for DNA detection". Materials Research Society Symposium Proceedings 872 (2005): 157-162. http://www.scopus.com/inward/record.url?eid=2-s2.0-30644465891&partnerID=MN8TOARS.
  156. Trindade, I.P.; Diogo, M.M.; Prazeres, D.M.F.; Marcos, J.C.. "Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography". Journal of Chromatography A 1082 2 (2005): 176-184. http://www.scopus.com/inward/record.url?eid=2-s2.0-20544442647&partnerID=MN8TOARS.
    10.1016/j.chroma.2005.05.079
  157. Moreira, K.A.; Diogo, M.; Prazeres, D.M.; De Lima Filho, J.L.; Figueiredo Porto, A.L.. "Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography". Brazilian Archives of Biology and Technology 48 SPEC. ISS. (2005): 113-117. http://www.scopus.com/inward/record.url?eid=2-s2.0-27944448818&partnerID=MN8TOARS.
  158. Diogo, M.M.; Queiroz, J.A.; Prazeres, D.M.F.. "Chromatography of plasmid DNA". Journal of Chromatography A 1069 1 (2005): 3-22. http://www.scopus.com/inward/record.url?eid=2-s2.0-15244358693&partnerID=MN8TOARS.
    10.1016/j.chroma.2004.09.050
  159. Teles, F.R.R.; Prazeres, D.M.F.; Lima-Filho, J.L.. "Trends in dengue diagnosis". Reviews in Medical Virology 15 5 (2005): 287-302. http://www.scopus.com/inward/record.url?eid=2-s2.0-25144436817&partnerID=MN8TOARS.
    10.1002/rmv.461
  160. Azevedo, A.M.; Prazeres, D.M.F.; Cabral, J.M.S.; Fonseca, L.P.. "Ethanol biosensors based on alcohol oxidase". Biosensors and Bioelectronics 21 2 (2005): 235-247. http://www.scopus.com/inward/record.url?eid=2-s2.0-22044453261&partnerID=MN8TOARS.
    10.1016/j.bios.2004.09.030
  161. Gonçalves, D.I.; Prazeres, D.M.; Chu, V.; Conde, J.P.. "Electrolyte-gate a-Si: H thin film transistors". Materials Research Society Symposium Proceedings 862 (2005): 623-628. http://www.scopus.com/inward/record.url?eid=2-s2.0-30544451431&partnerID=MN8TOARS.
  162. Fixe, F.; Branz, H.M.; Louro, N.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Electric-field assisted immobilization and hybridization of DNA oligomers on thin-film microchips". Nanotechnology 16 10 (2005): 2061-2071. http://www.scopus.com/inward/record.url?eid=2-s2.0-25444500191&partnerID=MN8TOARS.
    10.1088/0957-4484/16/10/014
  163. Fixe, F.; Branz, H.M.; Louro, N.; Chu, V.; Prazeres, D.M.F.; Conde, J.P.. "Immobilization and hybridization by single sub-millisecond electric field pulses, for pixel-addressed DNA microarrays". Biosensors and Bioelectronics 19 12 (2004): 1591-1597. http://www.scopus.com/inward/record.url?eid=2-s2.0-2442451209&partnerID=MN8TOARS.
    10.1016/j.bios.2003.12.012
  164. Fixe, F.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Optoelectronic detection of DNA molecules using an amorphous silicon photodetector". Materials Research Society Symposium Proceedings 820 (2004): 43-48. http://www.scopus.com/inward/record.url?eid=2-s2.0-14944365481&partnerID=MN8TOARS.
  165. Fixe, F.; Chu, V.; Prazeres, D.M.; Conde, J.P.. "An on-chip thin film photodetector for the quantification of DNA probes and targets in microarrays.". Nucleic acids research 32 9 (2004): http://www.scopus.com/inward/record.url?eid=2-s2.0-2442668010&partnerID=MN8TOARS.
  166. Serralha, F.N.; Lopes, J.M.; Lemos, F.; Ramôa Ribeiro, F.; Prazeres, D.M.F.; Aires-Barros, M.R.; Cabral, J.M.S.. "Application of factorial design to the study of an alcoholysis transformation promoted by cutinase immobilized on NaY zeolite and Accurel PA6". Journal of Molecular Catalysis B: Enzymatic 27 1 (2004): 19-27. http://www.scopus.com/inward/record.url?eid=2-s2.0-0346725817&partnerID=MN8TOARS.
    10.1016/j.molcatb.2003.09.005
  167. Fixe, F.; Branz, H.M.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Covalent immobilization of DNA and hybridization on microchips by microsecond electric field pulses". Materials Research Society Symposium Proceedings 820 (2004): 37-42. http://www.scopus.com/inward/record.url?eid=2-s2.0-14944340044&partnerID=MN8TOARS.
  168. Azevedo, A.M.; Cabral, J.M.S.; Prazeres, D.M.F.; Gibson, T.D.; Fonseca, L.P.. "Thermal and operational stabilities of Hansenula polymorpha alcohol oxidase". Journal of Molecular Catalysis B: Enzymatic 27 1 (2004): 37-45. http://www.scopus.com/inward/record.url?eid=2-s2.0-0346095156&partnerID=MN8TOARS.
    10.1016/j.molcatb.2003.09.001
  169. Ribiero, S.C.; Monteiro, G.A.; Prazeres, D.M.. "The role of polyadenylation signal secondary structures on the resistance of plasmid vectors to nucleases". Journal of Gene Medicine 6 5 (2004): 565-573. http://www.scopus.com/inward/record.url?eid=2-s2.0-13644257031&partnerID=MN8TOARS.
    10.1002/jgm.536
  170. Valente, C.A.; Prazeres, D.M.F.; Cabral, J.M.S.; Monteiro, G.A.. "Translational features of human alpha 2b interferon production in Escherichia coli". Applied and Environmental Microbiology 70 8 (2004): 5033-5036. http://www.scopus.com/inward/record.url?eid=2-s2.0-4143142271&partnerID=MN8TOARS.
    10.1128/AEM.70.8.5033-5036.2004
  171. Prazeres, D.M.F.; Ferreira, G.N.M.. "Design of flowsheets for the recovery and purification of plasmids for gene therapy and DNA vaccination". Chemical Engineering and Processing: Process Intensification 43 5 (2004): 615-630. http://www.scopus.com/inward/record.url?eid=2-s2.0-0347899375&partnerID=MN8TOARS.
    10.1016/j.cep.2003.02.002
  172. Fernandes, P.; Prazeres, D.M.; Cabral, J.M.; Fernandes, Pedro; Prazeres, Duarte M. F.; Cabral, Joaquim M. S.. "Membrane-assisted extractive bioconversions.". Advances in biochemical engineering/biotechnology 80 (2003): 115-148. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037712741&partnerID=MN8TOARS.
    10.1007/3-540-36782-9_4
  173. Diogo, M.M.; Prazeres, D.M.F.; Pinto, N.G.; Queiroz, J.A.. "Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B: Using and relating two different models for describing the effect of salt and temperature on retention". Journal of Chromatography A 1006 1-2 (2003): 137-148. http://www.scopus.com/inward/record.url?eid=2-s2.0-0042848639&partnerID=MN8TOARS.
    10.1016/S0021-9673(03)00918-X
  174. Diogo, M.M.; Queiroz, J.A.; Prazeres, D.M.F.. "Assessment of purity and quantification of plasmid DNA in process solutions using high-performance hydrophobic interaction chromatography". Journal of Chromatography A 998 1-2 (2003): 109-117. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038392599&partnerID=MN8TOARS.
    10.1016/S0021-9673(03)00618-6
  175. Martins, S.A.M.; Prazeres, D.M.F.; Cabral, J.M.S.; Monteiro, G.A.. "Comparison of real-time polymerase chain reaction and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products". Analytical Biochemistry 322 1 (2003): 127-129. http://www.scopus.com/inward/record.url?eid=2-s2.0-0141535030&partnerID=MN8TOARS.
    10.1016/j.ab.2003.07.004
  176. Fixe, F.; Cabeça, R.; Chu, V.; Prazeres, D.M.F.; Ferreira, G.N.M.; Conde, J.P.. "Electric-field-pulse-assisted covalent immobilization of dna in the nanosecond time scale". Applied Physics Letters 83 7 (2003): 1465-1467. http://www.scopus.com/inward/record.url?eid=2-s2.0-0041416286&partnerID=MN8TOARS.
    10.1063/1.1594839
  177. Prazeres, Duarte; Fixe, F.; Branz, H.M.; Prazeres, D.M.F.; Chu, V.; Conde, J.P.. "Electric field pulse assisted covalent immobilization and hybridization of DNA in the nanosecond time scale". Materials Research Society Symposium - Proceedings 773 (2003): 37-42. http://www.scopus.com/inward/record.url?eid=2-s2.0-1542274332&partnerID=MN8TOARS.
    10.1557/proc-773-n10.5
  178. Ribeiro, S.C.; Monteiro, G.A.; Cabral, J.M.S.; Prazeres, D.M.F.. "Isolation of plasmid DNA from cell lysates by aqueous two-phase systems". Biotechnology and Bioengineering 78 4 (2002): 376-384. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037141575&partnerID=MN8TOARS.
    10.1002/bit.10227
  179. Diogo, M.M.; Queiroz, J.A.; Prazeres, D.M.F.. "Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B Application of the solvophobic theory". Journal of Chromatography A 944 1-2 (2002): 119-128. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037168964&partnerID=MN8TOARS.
    10.1016/S0021-9673(01)01388-7
  180. León, R.; Prazeres, D.M.F.; Molinari, F.; Cabral, J.M.S.. "Microbial stereoselective oxidation of 2-methyl-1,3-propanediol to (R)-ß-hydroxyisobutyric acid in aqueous/organic biphasic systems". Biocatalysis and Biotransformation 20 3 (2002): 201-207. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036117613&partnerID=MN8TOARS.
    10.1080/10242420290020723
  181. Serralha, F.N.; Lopes, J.M.; Aires-Barros, M.R.; Prazeres, D.M.F.; Cabral, J.M.S.; Lemos, F.; Ramôa Ribeiro, F.. "Stability of a recombinant cutinase immobilized on zeolites". Enzyme and Microbial Technology 31 1-2 (2002): 29-34. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036644004&partnerID=MN8TOARS.
    10.1016/S0141-0229(02)00068-6
  182. Fixe, F.; Faber, A.; Gonçalves, D.; Prazeres, D.M.F.; Cabeça, R.; Chu, V.; Ferreira, G.; Conde, J.P.. "Thin film micro arrays with immobilized DNA for hybridization analysis". Materials Research Society Symposium - Proceedings 723 (2002): 125-130. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036961858&partnerID=MN8TOARS.
  183. Fernandes, P.; Vidinha, P.; Ferreira, T.; Silvestre, H.; Cabral, J.M.S.; Prazeres, D.M.F.. "Use of free and immobilized Pseudomonas putida cells for the reduction of a thiophene derivative in organic media". Journal of Molecular Catalysis B: Enzymatic 19-20 (2002): 353-361. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037010712&partnerID=MN8TOARS.
    10.1016/S1381-1177(02)00186-8
  184. Serralha, F.N.; Lopes, J.M.; Lemos, F.; Prazeres, D.M.F.; Aires-Barros, M.R.; Cabral, J.M.S.; Ramôa Ribeiro, F.. "Kinetics and modelling of an alcoholysis reaction catalyzed by cutinase immobilized on NaY zeolite". Journal of Molecular Catalysis - B Enzymatic 11 4-6 (2001): 713-718. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035931488&partnerID=MN8TOARS.
    10.1016/S1381-1177(00)00050-3
  185. Cabral, J.; Prazeres, D.M.. "Editorial: The Second International Conference on Protein Stabilisation, 9-12 April 2000, Lisbon". Biocatalysis and Biotransformation 19 5-6 (2001): http://www.scopus.com/inward/record.url?eid=2-s2.0-0035213735&partnerID=MN8TOARS.
  186. Prazeres, D.M.F.; Monteiro, G.A.; Ferreira, G.N.M.; Diogo, M.M.; Ribeiro, S.C.; Cabral, J.M.S.; Prazeres, D.M.F; et al. "Purification of plasmids for gene therapy and DNA vaccination". Biotechnology Annual Review 7 (2001): 1-30. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035237628&partnerID=MN8TOARS.
    10.1016/S1387-2656(01)07031-4
  187. León, R.; Prazeres, D.M.F.; Fernandes, P.; Molinari, F.; Cabral, J.M.S.. "A multiphasic hollow fiber reactor for the whole-cell bioconversion of 2-methyl-1,3-propanediol to (R)-ß-hydroxyisobutyric acid". Biotechnology Progress 17 3 (2001): 468-473. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034970887&partnerID=MN8TOARS.
    10.1021/bp010015g
  188. Diogo, M.M.; Ribeiro, S.C.; Queiroz, J.A.; Monteiro, G.A.; Tordo, N.; Perrin, P.; Prazeres, D.M.F.. "Production, purification and analysis of an experimental DNA vaccine against rabies". Journal of Gene Medicine 3 6 (2001): 577-584. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035523565&partnerID=MN8TOARS.
    10.1002/jgm.218
  189. Azevedo, A.M.; Prazeres, D.M.F.; Cabral, J.M.S.; Fonseca, L.P.. "Stability of free and immobilised peroxidase in aqueous-organic solvents mixtures". Journal of Molecular Catalysis - B Enzymatic 15 4-6 (2001): 147-153. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035501590&partnerID=MN8TOARS.
    10.1016/S1381-1177(01)00017-0
  190. Azevedo, A.M.; Fonseca, L.P.; Graham, D.L.; Cabral, J.M.S.; Prazeres, D.M.F.. "Behaviour of horseradish peroxidase in AOT reversed micelles". Biocatalysis and Biotransformation 19 3 (2001): 213-233. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034925825&partnerID=MN8TOARS.
  191. Serralha, F.N.; Lopes, J.M.; Vieira Ferreira, L.F.; Lemos, F.; Prazeres, D.M.F.; Aires-Barros, M.R.; Cabral, J.M.S.; Ramôa Ribeiro, F.. "Conformational changes induced by immobilization of a recombinant cutinase on zeolites". Catalysis Letters 73 1 (2001): 63-66. http://www.scopus.com/inward/record.url?eid=2-s2.0-0038227096&partnerID=MN8TOARS.
    10.1023/A:1016660913912
  192. Amaral, J.; Inganäs, M.; Cabral, J.; Prazeres, D.. "Study on the scale-up of human IgG3 purification using protein A affinity chromatography.". Bioseparation 10 4-5 (2001): 139-143. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035552335&partnerID=MN8TOARS.
  193. Diogo, M.M.; Queiroz, J.A.; Prazeres, D.M.. "Studies on the retention of plasmid DNA and Escherichia coli nucleic acids by hydrophobic interaction chromatography.". Bioseparation 10 4-5 (2001): 211-220. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035554023&partnerID=MN8TOARS.
  194. Azevedo, A.M.; Fonseca, L.P.; Prazeres, D.M.F.. "Kinetic and stability studies of penicillin acylase in reversed micelles". Biocatalysis and Biotransformation 17 6 (2000): 401-415. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033963093&partnerID=MN8TOARS.
  195. Ferreira, G.N.M.; Cabral, J.M.S.; Prazeres, D.M.F.. "Anion exchange purification of plasmid DNA using expanded bed adsorption". Bioseparation 9 1 (2000): 1-6. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034078460&partnerID=MN8TOARS.
    10.1023/A:1008134822673
  196. Diogo, M.M.; Ribeiro, S.; Queiroz, J.A.; Monteiro, G.A.; Perrin, P.; Tordo, N.; Prazeres, D.M.F.. "Scale-up of hydrophobic interaction chromatography for the purification of a DNA vaccine against rabies". Biotechnology Letters 22 17 (2000): 1397-1400. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033799808&partnerID=MN8TOARS.
    10.1023/A:1005665003375
  197. Serralha, F.N.; Lopes, J.M.; Lemos, F.; Prazeres, D.; Aires-Barros, M.; Rocha, J.; Cabral, J.; Ribeiro, F.R.. "Titanosilicates as supports for an enzymatic alcoholysis reaction". Reaction Kinetics and Catalysis Letters 69 2 (2000): 217-222. http://www.scopus.com/inward/record.url?eid=2-s2.0-0007679927&partnerID=MN8TOARS.
  198. Feliciano, A.S.; Dias, A.I.; Prazeres, D.M.F.. "The effect of stirring and seeding on the AcPheLeuNH2 synthesis and crystallization in a reversed micellar system". Enzyme and Microbial Technology 27 3-5 (2000): 264-269. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034255880&partnerID=MN8TOARS.
    10.1016/S0141-0229(00)00201-5
  199. Ferreira, G.N.M.; Cabral, J.M.S.; Prazeres, D.M.F.. "Studies on the batch adsorption of plasmid DNA onto anion-exchange chromatographic supports". Biotechnology Progress 16 3 (2000): 416-424. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034080617&partnerID=MN8TOARS.
    10.1021/bp0000196
  200. Ferreira, G.N.M.; Monteiro, G.A.; Prazeres, D.M.F.; Cabral, J.M.S.. "Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications". Trends in Biotechnology 18 9 (2000): 380-388. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034284422&partnerID=MN8TOARS.
    10.1016/S0167-7799(00)01475-X
  201. León, R.; Molinari, F.; Prazeres, D.M.F.; Cabral, J.M.S.. "Organic media selection for the extraction of ß-hydroxyisobutyric acid produced by microbial biotransformation". Journal of Chemical Technology and Biotechnology 75 7 (2000): 617-624. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034235833&partnerID=MN8TOARS.
    10.1002/1097-4660(200007)75:7<617::AID-JCTB110>3.0.CO;2-1
  202. Giordano, R.L.C.; Giordano, R.C.; Prazeres, D.M.F.; Cooney, C.L.. "Analysis of a Taylor-Poiseuille vortex flow reactor -- II: Reactor modeling and performance assessment using glucose-fructose isomerization as test reaction". Chemical Engineering Science 55 18 (2000): 3611-3626. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033949803&partnerID=MN8TOARS.
    10.1016/S0009-2509(00)00052-X
  203. Ribeiro, S.C.; Monteiro, G.A.; Martinho, G.; Cabral, J.M.S.; Prazeres, D.M.F.. "Quantitation of plasmid DNA in aqueous two-phase systems by fluorescence analysis". Biotechnology Letters 22 13 (2000): 1101-1104. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033943770&partnerID=MN8TOARS.
    10.1023/A:1005610113677
  204. Diogo, M.M.; Queiroz, J.A.; Monteiro, G.A.; Martins, S.A.M.; Ferreira, G.N.M.; Prazeres, D.M.F.. "Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography". Biotechnology and Bioengineering 68 5 (2000): 576-583. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034608587&partnerID=MN8TOARS.
    10.1002/(SICI)1097-0290(20000605)68:5<576::AID-BIT13>3.0.CO;2-5
  205. Molinari, F.; Gandolfi, R.; Aragozzini, F.; Leon, R.; Prazeres, D.M.F.. "Biotransformations in two-liquid-phase systems - Production of phenylacetaldehyde by oxidation of 2-phenylethanol with acetic acid bacteria". Enzyme and Microbial Technology 25 8-9 (1999): 729-735. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033230983&partnerID=MN8TOARS.
    10.1016/S0141-0229(99)00107-6
  206. Azevedo, A.M.; Fonseca, L.P.; Prazeres, D.M.F.. "Stability and stabilisation of penicillin acylase". Journal of Chemical Technology and Biotechnology 74 11 (1999): 1110-1116. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032714181&partnerID=MN8TOARS.
    10.1002/(SICI)1097-4660(199911)74:11<1110::AID-JCTB149>3.0.CO;2-B
  207. Diogo, M.M.; Queiroz, J.A.; Monteiro, G.A.; Prazeres, D.M.F.. "Separation and analysis of plasmid denatured forms using hydrophobic interaction chromatography". Analytical Biochemistry 275 1 (1999): 122-124. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033231382&partnerID=MN8TOARS.
    10.1006/abio.1999.4297
  208. Ferreira, G.N.M.; Cabral, J.M.S.; Prazeres, D.M.F.. "Monitoring of process streams in the large-scale production and purification of plasmid DNA for gene therapy applications". Pharmacy and Pharmacology Communications 5 1 (1999): 57-59. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032968972&partnerID=MN8TOARS.
  209. Serralheiro, M.L.M.; Prazeres, D.M.F.; Cabral, J.M.S.. "Continuous production and simultaneous precipitation of a dipeptide in a reversed micellar membrane reactor". Enzyme and Microbial Technology 24 8-9 (1999): 507-513. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032944832&partnerID=MN8TOARS.
    10.1016/S0141-0229(98)00141-0
  210. Prazeres, D.M.F.; Ferreira, G.N.M.; Monteiro, G.A.; Cooney, C.L.; Cabral, J.M.S.. "Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: Problems and bottlenecks". Trends in Biotechnology 17 4 (1999): 169-174. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033120652&partnerID=MN8TOARS.
    10.1016/S0167-7799(98)01291-8
  211. Ferreira, G.N.M.; Cabral, J.M.S.; Prazeres, D.M.F.. "Development of process flow sheets for the purification of supercoiled plasmids for gene therapy applications". Biotechnology Progress 15 4 (1999): 725-731. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033167256&partnerID=MN8TOARS.
    10.1021/bp990065+
  212. Monteiro, G.A.; Ferreira, G.N.M.; Cabral, J.M.S.; Prazeres, D.M.F.. "Analysis and use of endogenous nuclease activities in Escherichia coil lysates during the primary isolation of plasmids for gene therapy". Biotechnology and Bioengineering 66 3 (1999): 189-194. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033454713&partnerID=MN8TOARS.
    10.1002/(SICI)1097-0290(1999)66:3<189::AID-BIT7>3.0.CO;2-Z
  213. Molinari, F.; Villa, R.; Aragozzini, F.; Leon, R.; Prazeres, D.M.F.. "Enantioselective oxidation of (RS)-2-phenyl-1-propanol to (S)-2-phenylpropanoic acid with Gluconobacter oxydans: Simplex optimization of the biotransformation". Tetrahedron Asymmetry 10 15 (1999): 3003-3009. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033618599&partnerID=MN8TOARS.
    10.1016/S0957-4166(99)00309-2
  214. Giordano, R.C.; Giordano, R.L.C.; Prazeres, D.M.F.; Cooney, C.L.. "Analysis of a Taylor-Poiseuille vortex flow reactor-I: Flow patterns and mass transfer characteristics". Chemical Engineering Science 53 20 (1998): 3635-3652. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032538649&partnerID=MN8TOARS.
    10.1016/S0009-2509(98)00179-1
  215. Prazeres, D.M.F.; Schluep, T.; Cooney, C.. "Preparative purification of supercoiled plasmid DNA using anion- exchange chromatography". Journal of Chromatography A 806 1 (1998): 31-45. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032496250&partnerID=MN8TOARS.
    10.1016/S0021-9673(97)01254-5
  216. Serralha, F.N.; Lopes, J.M.; Lemos, F.; Prazeres, D.M.F.; Aires-Barros, M.R.; Cabral, J.M.S.; Ramôa Ribeiro, F.. "Zeolites as supports for an enzymatic alcoholysis reaction". Journal of Molecular Catalysis - B Enzymatic 4 5-6 (1998): 303-311. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032479932&partnerID=MN8TOARS.
    10.1016/S1381-1177(98)00069-1
  217. Ferreira, G.N.M.; Cabral, J.M.S.; Prazeres, D.M.F.. "Purification of supercoiled plasmid DNA using chromatographic processes". Journal of Molecular Recognition 11 1-6 (1998): 250-251. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032448825&partnerID=MN8TOARS.
  218. Feliciano, A.S.; Cabral, J.M.S.; Prazeres, D.M.F.. "Solubility studies and synthesis of AcPheLeuNH2 in reversed micellar systems". Biocatalysis and Biotransformation 14 3 (1997): 219-234. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030647952&partnerID=MN8TOARS.
  219. Cabral, J.M.S.; Aires-Barros, M.R.; Pinheiro, H.; Prazeres, D.M.F.. "Biotransformation in organic media by enzymes and whole cells". Journal of Biotechnology 59 1-2 (1997): 133-143. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031576709&partnerID=MN8TOARS.
    10.1016/S0168-1656(97)00176-4
  220. Molinari, F.; Aragozzini, F.; Cabral, J.M.S.; Prazeres, D.M.F.. "Continuous production of isovaleraldehyde through extractive bioconversion in a hollow-fiber membrane bioreactor". Enzyme and Microbial Technology 20 8 (1997): 604-611. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031172712&partnerID=MN8TOARS.
    10.1016/S0141-0229(96)00205-0
  221. Gonçalves, A.P.V.; Lopes, J.M.; Lemos, F.; Ramôa Ribeiro, F.; Prazeres, D.M.F.; Cabral, J.M.S.; Aires-Barros, M.R.. "Effect of the immobilization support on the hydrolytic activity of a cutinase from Fusarium solani pisi". Enzyme and Microbial Technology 20 2 (1997): 93-101. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031081636&partnerID=MN8TOARS.
    10.1016/S0141-0229(96)00089-0
  222. Feliciano, A.S.; Cabral, J.M.S.; Prazeres, D.M.F.. "Quantitative structure activity relationships in the synthesis of AcXYNH2, dipeptides by a-chymotrypsin in reversed micelles". Enzyme and Microbial Technology 21 4 (1997): 284-290. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031239662&partnerID=MN8TOARS.
    10.1016/S0141-0229(97)00043-4
  223. Prazeres, D.M.F.. "A theoretical analogy between multistage ultrafiltration and size-exclusion chromatography". Chemical Engineering Science 52 6 (1997): 953-960. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031082005&partnerID=MN8TOARS.
    10.1016/S0009-2509(96)00467-8
  224. Ferreira, G.N.M.; Cabral, J.M.S.; Prazeres, D.M.F.. "A comparison of gel filtration chromatographic supports for plasmid purification". Biotechnology Techniques 11 6 (1997): 417-420. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030968156&partnerID=MN8TOARS.
  225. Morgado, M.A.P.; Cabral, J.M.S.; Prazeres, D.M.F.. "Phospholipase A2-catalyzed hydrolysis of lecithin in a continuous reversed-micellar membrane bioreactor". JAOCS, Journal of the American Oil Chemists' Society 73 3 (1996): 337-346. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000531929&partnerID=MN8TOARS.
  226. Gonçalves, A.P.V.; Lopes, J.M.; Lemos, F.; Ramôa Ribeiro, F.; Prazeres, D.M.F.; Cabrai, J.M.S.; Aires-Barros, M.R.. "Zeolites as supports for enzymatic hydrolysis reactions. Comparative study of several zeolites". Journal of Molecular Catalysis B: Enzymatic 1 2 (1996): 53-60. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030568516&partnerID=MN8TOARS.
    10.1016/1381-1177(95)00013-5
  227. Prazeres, D.M.F.. "Optimal design of a number of membrane reactors in series using michaelis-menten one-substrate reversible kinetics". Biotechnology Techniques 10 5 (1996): 313-318. http://www.scopus.com/inward/record.url?eid=2-s2.0-0006258893&partnerID=MN8TOARS.
  228. Morgado, M.A.P.; Cabral, J.M.S.; Prazeres, D.M.F.. "Hydrolysis of lecithin by phospholipase A2 in mixed reversed micelles of lecithin and sodium dioctyl sulphosuccinate". Journal of Chemical Technology and Biotechnology 63 2 (1995): 181-189. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029003972&partnerID=MN8TOARS.
    10.1002/jctb.280630214
  229. Prazeres, D.M.. "Optimal design of N CSTRs in series using Michaelis-Menten one-substrate reversible reaction". Biocatalysis and Biotransformation 13 1 (1995): 43-52. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028817407&partnerID=MN8TOARS.
  230. Prazeres, D.M.F.. "Concentration of BSA using a superabsorbent polymer: Process evaluation". Journal of Biotechnology 39 2 (1995): 157-164. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028914416&partnerID=MN8TOARS.
    10.1016/0168-1656(95)00014-H
  231. Prazeres, D.M.F.. "Optimal design of N membrane reactors in series using Michaelis-Menten kinetics". Canadian Journal of Chemical Engineering 73 6 (1995): 931-934. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029482616&partnerID=MN8TOARS.
  232. Ribeiro, M.H.L.; Prazeres, D.M.F.; Cabral, J.M.S.; Da Fonseca, M.M.R.. "Adsorption studies for the separation of L-tryptophan from L-serine and indole in a bioconversion medium". Bioprocess Engineering 12 1-2 (1995): 95-102. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028896713&partnerID=MN8TOARS.
    10.1007/BF01112999
  233. Prazeres, D.M.F.; Garcia, F.A.P.; Cabral, J.M.S.. "Continuous lipolysis in a reversed micellar membrane bioreactor". Bioprocess Engineering 10 1 (1994): 21-27. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028055009&partnerID=MN8TOARS.
  234. Serralheiro, M.L.M.; Prazeres, D.M.F.; Cabral, J.M.S.. "Dipeptide synthesis and separation in a reversed micellar membrane reactor". Enzyme and Microbial Technology 16 12 (1994): 1064-1073. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028626281&partnerID=MN8TOARS.
    10.1016/0141-0229(94)90144-9
  235. Prazeres, D.M.F.; Cabral, J.M.S.. "Enzymatic membrane bioreactors and their applications". Enzyme and Microbial Technology 16 9 (1994): 738-750. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028500530&partnerID=MN8TOARS.
    10.1016/0141-0229(94)90030-2
  236. Prazeres, D.M.F.; Garcia, F.A.P.; Cabral, J.M.S.. "An ultrafiltration membrane bioreactor for the lipolysis of olive oil in reversed micellar media". Biotechnology and Bioengineering 41 8 (1993): 761-770. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027909203&partnerID=MN8TOARS.
  237. Prazeres, D.M.F.; Lemos, F.; Garcia, F.A.P.; Cabral, J.M.S.. "Modeling lipolysis in a reversed micellar system: Part I. Conventional batch reactor". Biotechnology and Bioengineering 42 6 (1993): 759-764. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027337621&partnerID=MN8TOARS.
  238. Prazeres, D.M.F.; Lemos, F.; Garcia, F.A.P.; Cabral, J.M.S.. "Modeling lipolysis in a reversed micellar system: Part II - Membrane reactor". Biotechnology and Bioengineering 42 6 (1993): 765-771. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027909876&partnerID=MN8TOARS.
  239. Prazeres, D.M.F.; Garcia, F.A.P.; Cabral, J.M.S.. "Colorimetric assays for the determination of dioctyl sodium sulphosuccinate and glycerol in reversed micelles". Biotechnology Techniques 7 1 (1993): 1-4. http://www.scopus.com/inward/record.url?eid=2-s2.0-0542439623&partnerID=MN8TOARS.
    10.1007/BF00151080
  240. Pires, M.J.; Prazeres, D.M.F.; Cabral, J.M.S.. "Protein assay in reversed micelle solutions". Biotechnology Techniques 7 4 (1993): 293-294. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000147642&partnerID=MN8TOARS.
    10.1007/BF00150901
  241. Prazeres, D.M.F.; Garcia, F.A.P.; Cabral, J.M.S.. "Kinetics and stability of a Chromobacterium viscosum lipase in reversed micellar and aqueous media". Journal of Chemical Technology and Biotechnology 53 2 (1992): 159-164. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026507204&partnerID=MN8TOARS.
Newspaper article
  1. Prazeres, Duarte. "Cura por edição génica", Jornal i, 2024, C, https://ionline.sapo.pt/artigo/809958.
  2. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "Testes em animais: o princípio do fim?", Jornal i, 2023, C, https://ionline.sapo.pt/artigo/808681.
  3. Prazeres, Duarte. "Uma pequena revolução privada", Jornal i, 2023, C, https://ionline.sapo.pt/artigo/ 807079.
  4. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "O fogo do génio", Jornal i, 2023, C, https://ionline.sapo.pt/artigo/798688.
  5. Prazeres, Duarte. "Cruzamento de plantas: Tradição e ciência", Jornal i, 2023, C, https://ionline.sapo.pt/artigo/794050.
  6. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "50 anos de quimeras", Jornal i, 2022, C, https://ionline.sapo.pt/artigo/788648.
  7. Prazeres, Duarte. "Donos de nós", Jornal i, 2022, C, https://ionline.sapo.pt/artigo/ 782334.
  8. Prazeres, Duarte. "Os devoradores de bactérias", Jornal i, 2022, C, https://ionline.sapo.pt/artigo/773679.
  9. Prazeres, Duarte. "O homem do braço de ouro", Jornal i, 2022, c, https://ionline.sapo.pt/artigo/764947.
  10. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "A patente do Dr. Chakrabarty", Jornal i, 2021, C, https://ionline.sapo.pt/artigo/755869.
  11. DMF Prazeres. "Os investigadores do inútil", Jornal i, 2021, C, https://ionline.sapo.pt/artigo/748279.
  12. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "O pão dos Faraós", Jornal i, 2021, C, https://ionline.sapo.pt/artigo/ 736407.
  13. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "Os animais duplicados", Jornal i, 2021, C, https://ionline.sapo.pt/artigo/726578.
  14. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "O mensageiro inesperado", Jornal i, 2020, C, https://ionline.sapo.pt/artigo/716787.
  15. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "À espera de uma vacina", CJornal i, 2020, C, https://ionline.sapo.pt/artigo/710841.
  16. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "Haja Sangue", Jornal i, 2020, C, https://ionline.sapo.pt/artigo/704802.
  17. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "Multum in parvo", Jornal i, 2020, C, https://ionline.sapo.pt/artigo/682858.
  18. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "Lei na Selva", Jornal i, 2020, C, https://ionline.sapo.pt/artigo/689272.
  19. Prazeres, Duarte. "Medicina de precisão", Jornal i, 2020, C, https://ionline.sapo.pt/artigo/683662.
  20. Prazeres, Duarte. "Conhecimento tradicional e biopirataria", Jornal i, 2019, C, https://ionline.sapo.pt/artigo/671330.
  21. Prazeres, Duarte. "Memórias de DNA", Jornal i, 2019, C, https://ionline.sapo.pt/artigo/663783.
  22. Prazeres, Duarte. "Agricultura celular", Jornal i, 2019, C, https://ionline.sapo.pt/artigo/658113.
  23. Prazeres, Duarte. "Desextinção: o regresso dos mamutes", Jornal i, 2019, C, https://ionline.sapo.pt/649653.
  24. Prazeres, Duarte. Corresponding author: Prazeres, Duarte. "O Mais Estranho dos Transplantes", Jornal i, 2019, C, https://ionline.sapo.pt/artigo/677534.
  25. Prazeres, Duarte Miguel. "DNA, genealogia e crime", Jornal i, 2018, C, https://ionline.sapo.pt/638967.
  26. Prazeres, Duarte Miguel. "A Idade do plástico", Jornal i, 2018, C, https://ionline.sapo.pt/631249.
  27. Prazeres, Duarte Miguel. "Nós e a água", Jornal i, 2018, C, https://ionline.sapo.pt/artigo/604043/nos-e-a-agua?seccao=Opiniao_i.
  28. Prazeres, Duarte Miguel. "O biomimetismo, inovação inspirada na natureza", Jornal i, 2018, C, https://ionline.sapo.pt/artigo/596448/-o-biomimetismo-inovacao-inspirada-na-natureza?seccao=Opiniao_i.
  29. Prazeres, Duarte Miguel. "O milagre da insulina", 2018, C, https://ionline.sapo.pt/612038.
  30. Prazeres, Duarte Miguel. "Na rota da seda de aranha", Jornal i, 2018, C, https://ionline.sapo.pt/619306.
  31. Prazeres, Duarte Miguel. "Vacinas, um imperativo social", Jornal i, 2017, https://ionline.sapo.pt/artigo/588823/vacinas-um-imperativo-social?seccao=Opiniao_i.
  32. Prazeres, Duarte Miguel. "O Advento da Terapia Génica", Jornal i, 2017, https://ionline.sapo.pt/artigo/579194/o-advento-da-terapia-genica?seccao=Opiniao_i.
Online resource
  1. DMF Prazeres; Pedro Miguel Matos Pereira; Ana Sofia Moita; Gabriel António Amaro Monteiro. Biomimetic replica of the upper epidermis of a gladiolus leaf. 2017. https://figshare.com/articles/figure/Biomimetic_replica_of_the_upper_epidermis_of_a_gladiolus_leaf/5615497.
    10.6084/m9.figshare.5615497
  2. Prazeres, Duarte. Crystals of enzymatically-synthesized AcPheLeuNH2 dipeptide. 2014. http://10.6084/m9.figshare.1185429.v1.
    10.6084/m9.figshare.1185429

Other

Dataset
  1. Pedro Miguel Matos Pereira; Ana Sofia Moita; Gabriel António Amaro Monteiro; DMF Prazeres. Biomimetic replicas of plants surfaces. 2023.
    10.6084/m9.figshare.1205058
  2. Prazeres, DMF. 3D-Printed Biomolecular Structures. 2020.
    https://doi.org/10.6084/m9.figshare.6115940.v1
  3. Prazeres, DMF; Ana Margarida Mendonça Rosa. Microscopic structure of wax-printed chromatographic paper (100X). 2015.
    https://doi.org/10.6084/m9.figshare.1609706.v1
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2023/04 - Current A novel generation of DNA polymerases for qPCR molecular diagnostics
Co-supervisor of Hélvio Miguel Simões
 Biotechnology and Biosciences (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019/09/01 - Current Biomanufacturing of Non-Viral Protein Nanocages
Supervisor of Jorge Fernandes da Silva João
Biotechnology and Biosciences (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/09 - 2023/03/01 Biomanufacturing of DNA-origami nanostructures for bio-imaging
Supervisor of Ana Rita Silva-Santos
 Biotechnology and Biosciences (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/09 - 2022/11/30 Nanoparticle-based (Bio)sensors
Supervisor of Rui Pedro Oliveira Silva
 Biotecnologia e Biociências (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/02 - 2021/11 Exploring carbohydrate binding modules in cellulose-based lateral flow assays
Supervisor of Maria Aguiar de Andrade Fontes
 Biological Engineering (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/09 - 2021/05 Escherichia coli Genome Engineering For Improved Minicircle Production
Co-supervisor of Cláudia Patrícia Alves
 Biotecnologia e Biociências (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2020/01 - 2020/10/14 Manufacturing of Vibrio natriegens cell lysates for cell-free protein synthesis
Supervisor of Marco André Pereira Gomes
 Ciências de Engenharia - Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2013/01 - 2020/01/29 DNA recognition system on bioactive paper using fusions of carbohydrate-binding modules and IgG-binding Z domains
Supervisor of Ana Margarida de Mendonça Rosa
 Biotechnology and Biosciences (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015/09 - 2019/12/30 Fluorescently-labeled DNA Probes Functionalized Onto Gold nanorods for Signal Enhancement in Nucleic Acids’ Detection
Co-supervisor of David José Roxo Botequim
 Biotecnologia e Biociências (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/09 - 2019/09/26 Development of paper-based biosensors for DNA recognition and proteolytic activity
Supervisor of Hélvio Miguel Rodrigues Simões
 Biotechnology (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019/01 - 2019/07/04 Research and evaluation of materials with antimicrobial properties for application in industrial kitchens
Supervisor of Luís Manuel Barradas Bento
 Ciências de Engenharia - Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014/06 - 2019/06/07 Ex-vivo Gene Therapy to Improve the Regenerative Features of Human Mesenchymal Stem/stromal Cells (MSC) in a Model of Limb Ischemia
Supervisor of Joana Catarina O. Pinto Serra
 Bioengenharia (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/01 - 2018/12/05 Modulating the Regenerative Features of Human Mesenchymal Stem/Stromal Cells with SDF-1a: A Gene Therapy Approach Using Minicircles
Supervisor of Tiago Miguel Ricardo Ligeiro
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/01 - 2017/11/20 Estudo e caracterização de processos de separação de sólidos aplicados em matérias-primas de fruta
Supervisor of Joana Filipa Gameiro De Mira
 Ciências de Engenharia - Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/01 - 2016/11/18 Development of Qualitative Lateral Flow Paper-based Microfluidic Devices for D-dimer Immunodetection
Supervisor of Sónia Cristina Martins Ruivo
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/01 - 2016/07/26 Paper-based Bioassays for Dengue Diagnostic Using Gold Nanoparticle Colorimetric Probes
Supervisor of Maria do Rosário Oliveira e Silva Queiroz Nazaré
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015/10 - 2016/06/21 Synthesis and Precipitation of Silica Nano-structures on Cellulose Fibers by Biosilicification Processes
Supervisor of Verónica de Carvalho Roque Bouça
 Bioengenharia e Nanossistemas (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015/01 - 2015/12/04 Immunodetection in Paper-based Devices Using Carbohydrate Binding Modules Fusions for Antibody Anchoring and Gold Nanoparticles for Colorimetric Reporting
Supervisor of António Ricardo Marques Almeida
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015/01 - 2015/12/04 A Multimodal Approach in the Downstream Processing of Plasmid DNA
Supervisor of Ana Rita da Silva Santos
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014/02 - 2014/12/12 Development of a Method for the Purification of Minicircles
Supervisor of Cláudia Patrícia Almeida Alves
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014/01 - 2014/11/27 Development of Rapid Tests for the Detection of L-malic Acid in Wine Using Enzymes Immobilized on Paper via Carbohydrate-binding Modules
Supervisor of Ana Catarina dos Reis Faria
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2010/09 - 2014/10 Hydrophobic Interaction Membrane Chromatography for the Downstream Processing of Plasmid DNA
Co-supervisor of Luís Raiado Pereira
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2009/11 - 2013/12 Impact of Plasmid Downstream Processing on Transient Transfection
Supervisor of Jonathan de La Vega Olivas
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2009/11 - 2013/12 Development of a Process for the Production and Purification of Minicircles for Biopharmaceutical Application
Co-supervisor of Michaela Simcikova
 Bioengenharia (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2013/01 - 2013/10 Biomimetic Replicas: Transfer of Complex Architectures from Plant Surfaces onto Polymeric Materials
Supervisor of Pedro Miguel Matos Pereira
 Engenharia Biológica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2009/03 - 2013/04 Rational Engineering of Escherichia coli Strains for Plasmid Biopharmaceutical Manufacturing
Supervisor of Geisa Aparecida Gonçalves Lopes
 Bioengineering (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2008/12 - 2013/01 Label-Free Electrical Detection of Biomolecules in Micro and Nanochannels
Co-supervisor of Diogo de Oliveira Calisto Garcia Martins
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2012/01 - 2012/09/27 Paper-based Microfluidics for Molecular Diagnostics: Immobilization of Biomolecules in Wax-Printed Microfluidic Channels via CBM3-ZZ Chimeric Protein
Supervisor of Ana Margarida de Mendonça Rosa
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2008/04 - 2012/05 Integration of Light Sensor Arrays with Microfluidic Networks to Scale Down 2D Fluorescence Spectroscopy for High Throughput Organism/Cell Condition Analysis
Co-supervisor of Agnieszka Jóskowiak
 Bioengineering (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2011/01 - 2011/10 Towards the miniaturization of cell assays for GPCR monitoring
Supervisor of João Rodrigo Cardoso Trabuco
 Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2007/04 - 2011/05 Intermediate recovery of plasmid DNA based on aqueous two-phase systems and phenyl boronate adsorption
Supervisor of Ana Gabriela Gonçalves Neves Gomes
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2006/11 - 2010/12 Integrated Amorphous Silicon Photodiodes for Chemiluminescence, Colorimetric and Fluorescence Protein Detection
Co-supervisor of Ana Teresa Fernandes Pereira
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2005/12 - 2010/01 Structural Instability in Plasmid DNA
Co-supervisor of Pedro Henrique Couto Oliveira
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2005/02 - 2009/03 Electric-field Assisted DNA Chips
Co-supervisor of Ricardo Manuel Marques Caleiro Cabeça
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2007/10 - 2008/12 Construction, Optimization and Testing of African Trypanosomiasis DNA Vaccine Prototypes with Improved Nuclease Resistance
Supervisor of Salomé Alexandra de Sá Magalhães
 Biologia Molecular e Genética (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2007/10 - 2008/12 Efeito do Grau de Superenrolamento de Plasmídeos na sua Estabilidade Estrutural e Função Biológica
Supervisor of Joana Brissos Magalhães Lima
 Biologia Molecular e Genética (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2004/08 - 2008/09 Affinity Chromatographic Processes in Nucleic Acids
Co-supervisor of Fani Pereira de Sousa
 Biochemistry (PhD)
Universidade da Beira Interior, Portugal
2004/06 - 2008/07 Label-free Detection of Biomolecules using Amorphous Silicon Ion-sensitive Field-effect Transistors
Co-supervisor of Dina Isabel Viegas Gonçalves
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2003/09 - 2007/10 Desenvolvimento e Optimização de Processos de Produção e Purificação de Plasmídeos para Utilização em Terapia Génica e Vacinação com DNA
Supervisor of Sindélia Silva de Freitas
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2006/09 - 2007/07 Controlo de Qualidade de Plasmídeos para Utilização em Terapia Génica e Vacinação com DNA
Supervisor of Alexandra Inverno
 Engenharia Bioquímica (Master)
Universidade de Coimbra, Portugal
2002/11 - 2006/12 Desenvolvimento de Vacinas de DNA para a Tripanossomose Humana Africana
Supervisor of Marcelo de Sousa da Silva
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2002/02 - 2006/03 Mapeamento de Hot Spots em Plasmídeos para Terapia Génica e Vacinação de DNA
Supervisor of Sofia Cabral de Andrade Duarte Ribeiro
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2001/09 - 2005/10 Design of Electronic Thin-film DNA Chips: Addressing and Detection
Co-supervisor of Ana Filipa Nogueira Fixe
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2001/04 - 2005/05 Desenvolvimento de Vacinas de DNA Contra o Vírus Maedi-Visna (MVV) dos Ovinos
Co-supervisor of Ana Margarida Ferreira Henriques
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2000/03 - 2004/04 Desenvolvimento de Processos Cromatográficos de Interacção Hidrofóbica para Análise e Purificação de Plasmídeos como Vectores de Terapia Génica e Vacinas de DNA
Supervisor of Maria Margarida Fonseca Rodrigues Diogo
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2000/09 - 2003/10 Fraccionamento de Plasma Sanguíneo por Cromatografia de Alta Resolução
Supervisor of José Esteves do Amaral
 PhD in Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
1999 - 2003/09 Síntese Enzimática de Oligopéptidos em Sistemas de Micelas Invertidas
Supervisor of Ana Sofia Feliciano Santos
 Biorechnology (PhD)
2000/06 - 2003/07 Produção de Interferão-2a – Construção de um Sistema de Expressão Bacteriano e Desenvolvimento de Processos de Purificação
Supervisor of Cláudia Alexandra dos Santos Valente
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
1997/01 - 2001/02 Modificação e Aplicação de Zeólitos em Transesterificações e Esterificações Enzimáticas em Solventes Não Aquosos
Co-supervisor of Maria de Fátima Nunes Serralha
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
1997 - 2000/01 Recuperação e Purificação de Plasmídeos de DNA Utilizando Processos Cromatográficos
Supervisor of Guilherme Matos Ferreira
 Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal

Committee member

Activity description
Role 		Institution / Organization
2023/01 - Current Vice-president of the Scientific Council of Instituto Superior Técnico.
President / Vice-president
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2023/01 - Current Coordinator of the Bioprocess Engineering Research Group at iBB-Institute for Bioengineering and Biosciences of Instituto Superior Técnico.
Coordinator
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/01 - Current Member of the Ethics Committee (EC) of Instituto Superior Técnico. The EC has the mission of promoting high ethical standards at IST. The EC may present proposals or recommendations in this regard consult on ethical issues raised related to teaching, scientific research, service provision to the community and functioning of the institution's services.
Member
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/01 - Current President of the Equivalency Committee of Instituto Superior Técnico.
President / Vice-president
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/01 - Current Member of the Scientific Council of Instituto Superior Técnico.
Member
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2014/01 - Current Member of the Executive Committee of iBB/Institute for Bioengineering and Biosciences of Instituto Superior Técnico.
Member
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2019/01 - 2022/12 Vice-President of the Department of Bioengineering of Instituto Superior Técnico, responsible for academic affairs.
President / Vice-president
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2013/11 - 2022/12 Coordinator of the Bioengineering Research Group at iBB-Institute for Bioengineering and Biosciences of Instituto Superior Técnico.
Coordinator
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2015/10 - 2020/12 Member of the School Council of Instituto Superior Técnico.
Member
2013/01 - 2018/12 Member of the Executive Comission of the Departament of Bioengineering of Instituto Superior Técnico, responsible for external relations
Member
2013/01 - 2015/10 Member of the Pedagogical Council of Instituto Superior Técnico.
Member
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2002/09 - 2005/08 Member of the Executive Commission of the Department of Chemical Engineering of Instituto Superior Técnico, responsible for the management of budget and personnel.
Member
 Universidade de Lisboa Instituto Superior Técnico, Portugal

Evaluation committee

Activity description
Role 		Institution / Organization Funding entity
2022/09 - 2022/09 Evaluator ERC, evaluator of a research proposal submitted to the Consolidator Grant 2022 call, European Research Council.
Evaluator
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2021 - 2021 Coordinator of Reviewer panel ARDITI, 2021 Call for Post-doctoral Fellowships, Agência Regional para o Desenvolvimento da Investigação, Tecnologia e Inovação.
Coordinator
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2019 - 2019 Coordinator of Reviewer panel ARDITI, 2019 Call for PhD Studentships and Post-doctoral Fellowships, Agência Regional para o Desenvolvimento da Investigação, Tecnologia e Inovação.
Coordinator
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/11 - 2018/12 Expert evaluator of the postdoctoral programme Junior Leader La Caixa (17 grants)
Evaluator
 Obra Social La Caixa, Spain Obra Social La Caixa
2018 - 2018 Expert Evaluator of Reviewer panel la Caixa, 2018 Postdoctoral Fellowships Junior Leader "la Caixa" - RETAINING programme. La Caixa Foundation.
Evaluator
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 - 2016 Coordinator of Reviewer panel ARDITI, 2016 Call for PhD Studentships and Post-doctoral Fellowships, Agência Regional para o Desenvolvimento da Investigação, Tecnologia e Inovação.
Coordinator
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2015 - 2015 Coordinator of Reviewer panel ARDITI, 2015 Call for PhD Studentships and Post-doctoral Fellowships, Agência Regional para o Desenvolvimento da Investigação, Tecnologia e Inovação.
Coordinator
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2013 - 2013 Reviewer FCT, 2013 Call for PhD Studentships and Post-doctoral Fellowships, area of Bioengineering and Biotechnology.
Evaluator
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2011 - 2011 Reviewer FCT, International relations projects, areas of Engineering Sciences and Technology, Biochemical Engineering/Biotechnology, Health Sciences and Chemistry
Evaluator
 Universidade de Lisboa Instituto Superior Técnico, Portugal
Distinctions

Other distinction

2010 Prémio Científico UTL/Santander Totta, na área de Engenharia Biológica, Bioquímica e Biotecnologia.
2009 Menção Honrosa no âmbito do Prémio Científico UTL/Santander Totta, na área de Engenharia Biológica, Bioquímica e Biotecnologia.
2001 Prémio 3M à Inovação na Área da Saúde