???global.info.a_carregar???
AM Azevedo graduated in 1996 in Chemical Engineering from Instituto Superior Técnico (IST) of the University of Lisbon. In 1999, she completed the MSc degree in Biochemical Engineering/Biotechnology and, in 2004, the PhD degree in Biotechnology, both at IST. In 2005, she started her post-doctoral studies and in 2008, she was hired as Research Scientist in the framework of Ciência 2007 and afterwards Investigador FCT. In 2010, she became Collaborating Lecturer at the Department of Bioengineering of IST; in 2016, Invited Assistant Professor; in 2018, Assistant Professor; and in 2022, Associate Professor. She has received 15 awards for Excellent Lecturer from the IST Pedagogical Council, including the IST Award Excellence in Teaching. She is also principal investigator of the Institute of Bioengineering and Biosciences (iBB) and Associate Laboratory, Institute for Health and Bioeconomy, where she leads the Bioseparation's Lab and coordinates several projects and PhD and MSc thesis. She has completed the supervision/co-supervision of 9 PhD students and 85 MSc students and is currently supervising 6 PhD students and 3 MSc students. Her current research interests are focused in Bioprocess Engineering, namely on the optimization, integration and intensification of bioprocess through miniaturization, high-throughput screening and continuous processing. AM Azevedo has co-authored more than 100 peer-reviewed papers with an h-index of 35 and with over 4200 citations. Her scientific network and internationalization is supported by publications with +150 distinct authors from 25 different national/international institutions. She has participated in 6 European projects and in 14 national projects; coordinated 5 research projects (2 M¿) and participated in 15 as team member, dealing with a total of 40 academic and 27 industrial partners. AM Azevedo has been part of organizing and steering committees of several international conferences namely BPP 2017, Affinity 2011, ProStab 201, ABC2 workshop, ESBES 2018, ISPPP 2021, ICB 2019 and BPP 2022. She has been session organizer in ACS-BIOT 2017, ICB IV, Recovery XIX. She has also been serving the scientific committee of several conferences, including ESBES 2018, BPP 2019, Microbiotec 2021, ISPPP 2021.
Read full resume ↓
Identification

Personal identification

Full name
Ana Margarida Nunes da Mata Pires de Azevedo
Gender
Female

Citation names

  • Azevedo, Ana
  • Azevedo, A.M.

Author identifiers

Ciência ID
221A-742B-4603
ORCID iD
0000-0001-5659-466X
Researcher Id
A-2985-2011
Scopus Author Id
25824143900

Email addresses

  • a.azevedo@ist.utl.pt (Professional)
  • a.azevedo@tecnico.ulisboa.pt (Professional)

Knowledge fields

  • Engineering and Technology - Industrial Biotechnology

Languages

Language Speaking Reading Writing Listening Peer-review
Spanish; Castilian Beginner (A1) Intermediate (B1) Beginner (A1)
German Beginner (A1) Beginner (A1) Beginner (A1) Beginner (A1)
French Beginner (A1) Intermediate (B1) Beginner (A1) Beginner (A1)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2004
Concluded
 Doutoramento em Biotecnologia (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Enzyme Stabilisation Studies for Biosensor Development" (THESIS/DISSERTATION)
 Aprovado
1999
Concluded
 Mestrado em Biotecnologia (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Estudos de estabilidade da penicilina acilase em meio aquoso e em micelas invertidas" (THESIS/DISSERTATION)
 17
1996
Concluded
 Engenharia Química (Licenciatura)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"n/a" (THESIS/DISSERTATION)
 15
Affiliation

Science

Category
Host institution 		Employer
2015/02/01 - 2018/06/30 Auxiliary Researcher (Research) Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2014/02/01 - 2015/01/31 Researcher (Research) Universidade de Lisboa Instituto Superior Técnico, Portugal
2008 - 2013 Auxiliary Researcher (Research) Instituto de Biotecnologia e Bioengenharia, Portugal

Teaching in Higher Education

Category
Host institution 		Employer
2022/05/01 - Current Associate Professor (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/07/01 - 2022/04/30 Assistant Professor (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/10/01 - 2018/06/30 Invited Assistant Professor (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal

Others

Category
Host institution 		Employer
2004/09/01 - 2008/09/30 Pos-Doc Researcher Universidade de Lisboa Instituto Superior Técnico, Portugal
1999/01/01 - 2004/07/22 Ph.D Student Universidade de Lisboa Instituto Superior Técnico, Portugal
1996/09/15 - 1999/12/31 MSc Student Universidade de Lisboa Instituto Superior Técnico, Portugal
Projects

Grant

Designation Funders
2024/02/01 - 2027/06/30 DIG4BIO - Digitalization of Biomanufacturing of Plasmids for the development of Advanced Therapy Modalities
101159993
Principal investigator
Universidade de Lisboa Instituto Superior Técnico, Portugal
 European Research Executive Agency
Ongoing

Contract

Designation Funders
2023/01/01 - 2026/12/31 Enabling worldwide vaccination by improving the mRNA quality in the manufacturing process
FE174/012023/47901
Supervisor
ModernaTX
Ongoing
2021/01/01 - 2025/12/31 Institute for Health and Bioeconomy
LA/P/0140/2020
10.54499/LA/P/0140/2020
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

INESC Microsistemas e Nanotecnologias, Portugal

Universidade NOVA de Lisboa, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2022/01/01 - 2024/12/31 Clean Technologies for Pure Water: Eliminating micropollutants from drinking water
PTDC/EAM-AMB/2023/2021
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Centro de Química Estrutural, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2022/01/01 - 2024/12/31 Plataforma de produção de células NK modificadas com CAR e suas vesículas extracelulares para imunoterapia
PTDC/EQU-EQU/3708/2021
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Instituto Politécnico de Lisboa Instituto Superior de Engenharia de Lisboa, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2019/01/01 - 2023/12/31 Applied Molecular Biosciences Unit
UIDP/04378/2020
10.54499/UIDP/04378/2020
UIDB/04378/2020
10.54499/UIDB/04378/2020
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Universidade Nova de Lisboa, Portugal

Universidade do Porto, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal

Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

INESC Microsistemas e Nanotecnologias, Portugal

Universidade Nova de Lisboa Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2019/02/01 - 2023/01/31 CODOBIO – Continuous Downstream Processing of Bioproducts
H2020-MSCA-ITN-2018-812909
Principal investigator
EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions
Ongoing
2018/10 - 2021/09 EXOPRO - Development of a scalable manufacturing process for therapeutic human mesenchymal stem/stromal cell-derived exosomes
PTDC/EQU-EQU/31651/2017
 Fundação para a Ciência e a Tecnologia
2018/10 - 2021/09 The aggregation-prone structures of Huntingtin exon 1 at the single-molecule level: Influence of membranes and implications in Huntington disease
PTDC/BIA-BFS/30959/2017
 Fundação para a Ciência e a Tecnologia
2018/09 - 2021/08 CBM-Z - Biorecognition as a Tool for the Functionalization of Cellulose-based Materials with Biomolecules and Nanostructures
PTDC/CTM-CTM/30790/2017
 Fundação para a Ciência e a Tecnologia
2018/05 - 2020/07 Phage4BacID – Bacteriófagos no diagnóstico e prevenção de infecções multirresistentes em Portugal e no mundo
LISBOA-01-0247-FEDER-03303
 Agência Nacional de Inovação SA
2019/01/01 - 2019/12/31 Institute for Bioengineering and Biosciences
UID/BIO/04565/2019
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016/01/01 - 2019/03/31 OptLOC - Integrated optical strategies for advanced Lab on Chip biosensors
PTDC/BBB-NAN/5927/2014
Researcher
INESC Microsistemas e Nanotecnologias, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016/01/01 - 2019/03/31 Estratégias ópticas integradas para biosensores em sistemas "Lab on Chip" avançadas
PTDC/BBB-NAN/5927/2014
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

INESC Microsistemas e Nanotecnologias, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016/01 - 2018/12 PureMAb - Multimodal chromatography as an alternative to pure chromatographic modes for the capture and polishing of antibody streams
Provided by PTCRIS: 138876
PTDC/QEQ-PRS/0286/2014
Principal investigator
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2012/08/01 - 2017/01/31 INTENSO – Gaining productivity, cost efficiency and sustainability in the downstream processing of bio-products by novel integration and intensification strategies
FP7-KBBE-2012-6-312004
FP7-KBBE-2012-6-312004
Researcher
Universidade de Lisboa Instituto Superior Técnico, Portugal
 European Commission Seventh Framework Programme for Research and Technological Development
Concluded
2012/01 - 2014/12 DSPMab - Intensification of the downstream process of monoclonal antibodies
117427
Provided by PTCRIS: 117427
PTDC/EQU-EPR/117427/2010
PTDC/EQU-EPR/117427/2010
Principal investigator
Instituto de Biotecnologia e Bioengenharia, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
2011/01 - 2013/12 Protein-Aqueous Environment Interactions
Provided by PTCRIS: 112812
112812
PTDC/EQU-EQU/112812/2009
PTDC/EQU-EQU/112812/2009
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2010/01 - 2013/12 Innovative strategies for the purification of biopharmaceuticals: Magnetic Nanoparticles applied to High-Gradient-Magnetic-Separation and Aqueous-Two-Phase-Systems
98961
Provided by PTCRIS: 98961
PTDC/EBB-BIO/098961/2008
Researcher
Universidade de Lisboa Instituto Superior Técnico, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2011/01/01 - 2012/12/31 Projecto Estratégico - LA 23 - 2011-2012
PEst-OE/EQB/LA0023/2011
Instituto de Biotecnologia e Bioengenharia, Portugal

Universidade do Algarve, Portugal

Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal

Universidade de Trás-os-Montes e Alto Douro, Portugal

Universidade do Minho, Portugal

Fundação Gaspar Frutuoso, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade dos Açores, Portugal

Universidade de Lisboa Instituto Superior Técnico, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2010/01 - 2012/12 STEMSELECT - Novel integrated process for human hematopoietic stem cell isolation and ex-vivo expansion
Provided by PTCRIS: 104663
104663
PTDC/EQU-EPR/104663/2008
Researcher
Universidade de Lisboa Instituto Superior Técnico, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
2007/10 - 2010/12 Biological and Molecular Characterization of Avian Influenza Viruses and Development of DIVA Tests
67189
Provided by PTCRIS: 67189
PTDC/CVT/67189/2006
PTDC/CVT/67189/2006
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2004/04/01 - 2008/10/31 AIMS – Advanced interactive materials by design
NMP3-CT2004-500160
Researcher
Universidade de Lisboa Instituto Superior Técnico, Portugal
 European Commission Sixth Framework Programme
Concluded
1999/01/05 - 2000/12/31 DIAMONDS – Directly interfaced and micro or nanostructured detection systems
BIO4-CT97-2199
PhD Student Fellow
Concluded
Outputs

Publications

Book chapter
  1. Wagner, Alexandra; Silva-Santos, A. Rita; Rosa, Sara Sousa; Gierak, Sophie; Azevedo, Ana M.; Prazeres, Duarte Miguel F.. "Primary Purification of Plasmid DNA Using Differential Isopropanol Precipitation". In Methods in Molecular Biology, 151-165. New York, United States: Springer US, 2021.
    Accepted • 10.1007/978-1-0716-0872-2_8
  2. Silva-Santos, A. Rita; Rosa, Sara Sousa; Prazeres, Duarte Miguel F.; Azevedo, Ana M.. "Purification of Plasmid DNA by Multimodal Chromatography". In Methods in Molecular Biology, 193-205. New York, United States: Springer US, 2021.
    Accepted • 10.1007/978-1-0716-0872-2_10
  3. Faria, Diogo F.; Silva, Tiago P.; Aires-Barros, M. Raquel; Azevedo, Ana M.. "A Chronology of the Development of Aqueous Two-Phase Systems as a Viable Liquid-Liquid Extraction for Biological Products". In Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. Elsevier, 2019.
    10.1016/b978-0-12-409547-2.14393-8
  4. Aires-Barros, M.R.; Azevedo, A.M.. "Fundamentals of Biological Separation Processes". In Current Developments in Biotechnology and Bioengineering, 187-237. Elsevier, 2017.
    Accepted • 10.1016/b978-0-444-63668-3.00007-x
  5. Capela, E.V.; Aires-Barros, M.R.; Mara G. Freire; Azevedo, A.M.. "Monoclonal Antibodies ¿ Addressing the Challenges on the Manufacturing Processing of an Advanced Class of Therapeutic Agents". In Frontiers in Clinical Drug Research-Anti-Infectives. Bentham Science, 2017.
  6. Ahmed Samatou, Joachim; Engbert Wentink, Annebart; Alexandra J.Rosa, Paula; Margarida Azevedo, Ana; Raquel Aires-Barros, Maria; Bäcker, Werner; Górak, Andrzej. "Modeling of counter current monoclonal antibody extraction using aqueous two-phase systems". In 17th European Symposium on Computer Aided Process Engineering, 935-940. Elsevier, 2007.
    Accepted • 10.1016/s1570-7946(07)80180-5
  7. Azevedo, Ana M.; Martins, Verónica C.; Prazeres, Duarte M.F.; Vojinovic, Vojislav; Cabral, Joaquim M.S.; Fonseca, Luís P.; Azevedo, A.M.; et al. "Horseradish peroxidase: a valuable tool in biotechnology". In Biotechnology Annual Review, 199-247. Elsevier, 2003.
    Accepted • 10.1016/s1387-2656(03)09003-3
Conference abstract
  1. Fernandes-Platzgummer, Ana; Rosa, Sara; Silva, Ricardo M.; Cunha, Raquel; Fuzeta, Miguel de Almeida ; Calado, Cecília Ribeiro da Cruz ; Cabral, Joaquim; Azevedo, Ana; Silva, Cláudia. "Scaling-up the manufacturing of well- characyerized mesenchumal stromal cell-derived extracellular vesicles for biomedical applications". Paper presented in International Society for Extracellular Vesicles Meeting 2020 (Virtual), Philadelphia, 2020.
  2. Fernandes-Platzgummer, Ana; Rosa, Sara; Silva, Ricardo M.; Cunha, Raquel; Fuzeta, Miguel de Almeida ; Calado, Cecília Ribeiro da Cruz ; Cabral, Joaquim; Azevedo, Ana; Silva, Cláudia. "Scaling-up the manufacturing of well- characterized mesenchymal stromal cell-derived extracellular vesicles for biomedical applications". Paper presented in International Society for Extracellular Vesicles Meeting 2020 (Virtual), Philadelphia, 2020.
Conference paper
  1. Soares, R.R.G.; Santos, D.R.; Pinto, I.F.; Azevedo, A.M.; Aires-Barros, M.R.; Chu, V.; Conde, J.P.. "Multiplexed microfluidic platform coupled with photodetector array for point-of-need and sub-minute detection of food contaminants". 2018.
    10.1109/MEMSYS.2018.8346467
  2. Pinto, I.F.; Santos, D.R.; Petrucci, G.; Soares, R.R.G.; Aires-Barros, M.R.; Chu, V.; Conde, J.P.; Azevedo, A.M.. "Development of a microfluidic device with integrated valves and photodiodes for rapid optimization of chromatographic separations of monoclonal antibodies". 2016.
  3. Soares, R.R.G.; Pinto, I.F.; Santos, D.R.; Azevedo, A.M.; Chu, V.; Aires-Barros, M.R.; Conde, J.P.. "A novel point-of-use, ultrafast and sensitive fluorescence immunoassay for the simultaneous detection of multiple mycotoxins using nanoporous agarose beads confined in microchannels". 2016.
  4. Soares, R.R.G.; Santos, D.R.; Pinto, I.F.; Azevedo, A.M.; Chu, V.; Aires-Barros, M.R.; Conde, J.P.. "Point-of-use Ultrafast Single-step Detection of Food Contaminants: A Novel Microfluidic Fluorescence-based Immunoassay with Integrated Photodetection". 2016.
    10.1016/j.proeng.2016.11.208
  5. Pinto, I.F.; Santos, D.R.; Soares, R.R.G.; Aires-Barros, M.R.; Chu, V.; Azevedo, A.M.; Conde, J.P.. "Integration of Photosensors in a Nano-liter Scale Chromatography Column for the Online Monitoring of Adsorption/Desorption Kinetics of a Fluorophore-labeled Monoclonal Antibody". 2016.
    10.1016/j.proeng.2016.11.405
  6. Jacinto, M.J.; Azevedo, A.M.; Aires-Barros, M.R.; Archinti, M.; Marzabal, P.. "Extraction of Zera® fusion proteins in aqueous two-phase systems". 2015.
    10.1109/ENBENG.2015.7088835
  7. Soares, R.; Silva, D.F.C.; Azevedo, A.M.; Fernandes, P.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "ATPS from natural products to large biomolecules". 2013.
  8. Azevedo, A.M.; Aires-Barros, M.R.. "Novel strategies for the purification of monoclonal antibodies". 2011.
    10.1109/ENBENG.2011.6026067
  9. Azevedo, A.M.; Aires-Barros, M.R.. "New platforms for the downstream processing of biopharmaceuticals". 2011.
    10.1109/ENBENG.2011.6026043
Conference poster
  1. Silva, Ricardo M.; Rosa, Sara; Silva, Cláudia; Fernandes-Platzgummer, Ana; Azevedo, Ana. "A chromatography approach for clinical-grade purification of MSC-derived extracellular vesicles". Paper presented in Microbiotec 2019, Coimbra, Portugal, 2019.
  2. P C de Barros, Dragana; Campos, Sara R. R.; Madeira, Pedro; Azevedo, Ana; Baptista, A M; Aires-Barros, M. R. "Predictability of the protein partitioning in aqueous two-phase systems using semi-empirical model". Paper presented in Encontro de Jovens Investigadores de Biologia Computacional Estrutural, 2016.
  3. P C de Barros, Dragana; Campos, Sara R. R.; Azevedo, Ana; Baptista, António; Aires-Barros, M. R.. "Protein partitioning in aqueous two phase system using a semi-empirical model". Paper presented in Encontro de Jovens Investigadores de Biologia Computacional e Estrutural, Lisbon, Portugal, 2014.
  4. P C de Barros, Dragana; Campos, Sara R. R.; Azevedo, Ana; Baptista, A.M.; Aires-Barros, M.R.. "Prediction of protein partition coefficients in aqueous two phase system". Paper presented in 34th International Symposium on the Separation of Proteins, Peptides and Polynucleotides, Würzburg , Germany, 2014.
  5. P C de Barros, Dragana; Campos, Sara R. R.; Madeira, Pedro; Azevedo, Ana; Baptista, A.M.; Aires-Barros, M.R.. "Study of a protein partitioning in aqueous two-phase system using a solvent continuum model". Paper presented in 9th International Conference on Protein Stabilisation,Lisbon, Portugal., 2012.
Journal article
  1. Jungbauer, Alois; Satzer, Peter; Duerauer, Astrid; Azevedo, Ana; Aires-Barros, Raquel; Nilsson, Bernt; Farid, Suzy; et al. "Continuous downstream processing". Separation and Purification Technology 338 (2024): 126439. http://dx.doi.org/10.1016/j.seppur.2024.126439.
    Open access • Published • 10.1016/j.seppur.2024.126439
  2. Malik Abdul Wahab; C. Domingues; Ana M. Azevedo; Virginia Chu; Joao Pedro Conde; M. Raquel Aires-Barros; Wahab, Malik Abdul; et al. "An integrated microfluidic device for continuous bioprocessing". Separation and Purification Technology 332 (2024): 125702. https://doi.org/10.1016/j.seppur.2023.125702.
    Published • 10.1016/j.seppur.2023.125702
  3. Fradique, Ricardo; Jardim, Andreia; Azevedo, Ana M; Chu, Virginia; Conde, João P; Raquel Aires-Barros, M. "Continuous microfluidic platform combining cell lysis and protein extraction for screening overall process conditions". Journal of Chemical Technology & Biotechnology 99 3 (2024): 618-625. http://dx.doi.org/10.1002/jctb.7564.
    Published • 10.1002/jctb.7564
  4. Diogo Ferreira-Faria; Filipa Domingos-Moreira; M. Raquel Aires-Barros; António Ferreira; Ana M. Azevedo. "Continuous precipitation of antibodies using oscillatory flow reactor: A proof of concept". Separation and Purification Technology (2023): https://doi.org/10.1016/j.seppur.2023.123924.
    10.1016/j.seppur.2023.123924
  5. Capela, Emanuel V.; Magnis, Ilaria; Rufino, Ana F.C.S.; Torres-Acosta, Mario A.; Aires-Barros, M. Raquel; Coutinho, João A.P.; Azevedo, Ana M.; e Silva, Francisca A.; Freire, Mara G.. "Using three-phase partitioning for the purification and recovery of antibodies from biological media". Separation and Purification Technology 316 (2023): 123823. http://dx.doi.org/10.1016/j.seppur.2023.123823.
    Published • 10.1016/j.seppur.2023.123823
  6. Silva, Ricardo M.; Rosa, Sara Sousa; Cunha, Raquel; L. da Silva, Cláudia; Azevedo, Ana M.; Fernandes-Platzgummer, Ana. "Anion exchange chromatography-based platform for the scalable purification of extracellular vesicles derived from human mesenchymal stromal cells". Separation and Purification Technology 310 (2023): 123238. http://dx.doi.org/10.1016/j.seppur.2023.123238.
    Published • 10.1016/j.seppur.2023.123238
  7. Capela, Emanuel V.; Bairos, Jéssica; Pedro, Augusto Q.; Neves, Márcia C.; Raquel Aires-Barros, M.; Azevedo, Ana M.; Coutinho, João A.P.; Tavares, Ana P.M.; Freire, Mara G.. "Supported ionic liquids as customizable materials to purify immunoglobulin G". Separation and Purification Technology 305 (2023): 122464. http://dx.doi.org/10.1016/j.seppur.2022.122464.
    10.1016/j.seppur.2022.122464
  8. Melo, Ana M.; Sousa, Tania; Damas, Goncalo; Coutinho, Ana; Bernardes, Nuno; Azevedo, Ana; Prieto, Manuel. "Probing the distinct conformational signatures of the flanking polyQ regions in huntingtin exon 1 at the membrane interface: insights into Huntington's disease". European Biophysics Journal (2023): https://publons.com/wos-op/publon/62961254/.
  9. Javidanbardan, Amin; Chu, Virginia; Conde, João P.; Azevedo, Ana M.. "Microchromatography integrated with impedance sensor for bioprocess optimization: Experimental and numerical study of column efficiency for evaluation of scalability". Journal of Chromatography A 1661 (2022): 462678. http://dx.doi.org/10.1016/j.chroma.2021.462678.
    Open access • Published • 10.1016/j.chroma.2021.462678
  10. Rosa, S.S.; Nunes, D.; Antunes, L.; Prazeres, D.M.F.; Marques, M.P.C.; Azevedo, A.M.. "Maximizing mRNA vaccine production with Bayesian optimization". Biotechnology and Bioengineering 119 11 (2022): 3127-3139. http://www.scopus.com/inward/record.url?eid=2-s2.0-85137330207&partnerID=MN8TOARS.
    10.1002/bit.28216
  11. Javidanbardan, Amin; Azevedo, Ana M.; Chu, Virginia; Conde, João P.; Amin Javidanbardan; Ana M. Azevedo; Virginia Chu; João P. Conde. "A Systematic Approach for Developing 3D High-Quality PDMS Microfluidic Chips Based on Micromilling Technology". Micromachines 13 1 (2021): 6. http://dx.doi.org/10.3390/mi13010006.
    10.3390/mi13010006
  12. Nascimento, André; São Pedro, Mariana N.; Pinto, Inês F.; Aires-Barros, Maria Raquel; Azevedo, Ana M.. "Microfluidics as a high-throughput solution for chromatographic process development – The complexity of multimodal chromatography used as a proof of concept". Journal of Chromatography A 1658 (2021): 462618. http://dx.doi.org/10.1016/j.chroma.2021.462618.
    Published • 10.1016/j.chroma.2021.462618
  13. Jacinto, M.J.; Wagner, Alexandra; Sá, Inês M.; Patinha, David J.S.; Marrucho, Isabel M.; Gonçalves, João; Willson, Richard C.; Azevedo, A.M.; Aires-Barros, M.R.. "Screening polymeric ionic liquids for chromatography-based purification of bacteriophage M13". Separation and Purification Technology 257 (2021): 117906. http://dx.doi.org/10.1016/j.seppur.2020.117906.
    Published • 10.1016/j.seppur.2020.117906
  14. Rosa, S.S.; Prazeres, D.M.F.; Azevedo, A.M.; Marques, M.P.C.. "mRNA vaccines manufacturing: Challenges and bottlenecks". Vaccine 39 16 (2021): 2190-2200. http://www.scopus.com/inward/record.url?eid=2-s2.0-85103093189&partnerID=MN8TOARS.
    10.1016/j.vaccine.2021.03.038
  15. João, J.; Lampreia, J.; Prazeres, D.M.F.; Azevedo, A.M.. "Manufacturing of bacteriophages for therapeutic applications". Biotechnology Advances 49 (2021): http://www.scopus.com/inward/record.url?eid=2-s2.0-85104913379&partnerID=MN8TOARS.
    10.1016/j.biotechadv.2021.107758
  16. Roque, Ana Cecília Afonso; Pina, Ana Sofia; Azevedo, Ana Margarida; Aires-Barros, Raquel; Jungbauer, Alois; Di Profio, Gianluca; Heng, Jerry Y. Y.; Haigh, Jonathan; Ottens, Marcel. "Anything but Conventional Chromatography Approaches in Bioseparation". Biotechnology Journal 15 8 (2020): 1900274. http://dx.doi.org/10.1002/biot.201900274.
    Published • 10.1002/biot.201900274
  17. Ferreira-Faria, D.; Aires-Barros, M.R.; Azevedo, A.M.. "Continuous aqueous two-phase extraction: From microfluidics to integrated biomanufacturing". Fluid Phase Equilibria 508 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85078093920&partnerID=MN8TOARS.
    10.1016/j.fluid.2019.112438
  18. Fradique, R.; Azevedo, A.M.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "Microfluidic platform for rapid screening of bacterial cell lysis". Journal of Chromatography A 1610 (2020): http://www.scopus.com/inward/record.url?eid=2-s2.0-85072291609&partnerID=MN8TOARS.
    10.1016/j.chroma.2019.460539
  19. Alexandra Wagner; Ana M Ferraria; Ana MB Do Rego; Marília Mateus; Ana M Azevedo. "Purification of monoclonal antibodies in a stirred cell with polyethyleneimine-modified polyethersulfone ultrafiltration membrane". Journal of Chemical Technology & Biotechnology (2019): https://doi.org/10.1002/jctb.6157.
    10.1002/jctb.6157
  20. Inês F. Pinto; Ruben R. G. Soares; Maria R. Aires-Barros; Virginia Chu; João P. Conde; Ana M. Azevedo. "Optimizing the Performance of Chromatographic Separations Using Microfluidics: Multiplexed and Quantitative Screening of Ligands and Target Molecules". Biotechnology Journal (2019): 1800593-1800593. https://doi.org/10.1002/biot.201800593.
    10.1002/biot.201800593
  21. Silva-Santos, A.R.; Alves, C.P.A.; Monteiro, G.; Azevedo, A.M.; Prazeres, D.M.F.. "Multimodal chromatography of supercoiled minicircles: A closer look into DNA-ligand interactions". Separation and Purification Technology (2019): 161-170. http://www.scopus.com/inward/record.url?eid=2-s2.0-85056601221&partnerID=MN8TOARS.
    10.1016/j.seppur.2018.11.015
  22. Jacinto, M.J.; Trabuco, J.R.C.; Vu, B.V.; Garvey, G.; Khodadadi, M.; Azevedo, A.M.; Aires-Barros, M.R.; et al. "Correction: Enhancement of lateral flow assay performance by electromagnetic relocation of reporter particles (PLoS One (2018) 13:1 (e0186782) DOI: 10.1371/journal.pone.0186782)". PLoS ONE 14 3 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85062878241&partnerID=MN8TOARS.
    10.1371/journal.pone.0213859
  23. Caneira, C.R.F.; Soares, R.R.G.; Pinto, I.F.; Mueller-Landau, H.S.; Azevedo, A.M.; Chu, V.; Conde, J.P.. "Development of a rapid bead-based microfluidic platform for DNA hybridization using single- and multi-mode interactions for probe immobilization". Sensors and Actuators, B: Chemical (2019): 328-336. http://www.scopus.com/inward/record.url?eid=2-s2.0-85061046188&partnerID=MN8TOARS.
    10.1016/j.snb.2019.01.133
  24. Rosa, S.A.S.L.; Wagner, A.; da Silva, C.L.; Aires-Barros, M.R.; Azevedo, A.M.; Dias-Cabral, A.C.. "Mobile-Phase Modulators as Salt Tolerance Enhancers in Phenylboronate Chromatography: Thermodynamic Evaluation of the Mechanisms Underlying the Adsorption of Monoclonal Antibodies". Biotechnology Journal 14 10 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85070666684&partnerID=MN8TOARS.
    10.1002/biot.201800586
  25. São Pedro, M.N.; Azevedo, A.M.; Aires-Barros, M.R.; Soares, R.R.G.. "Minimizing the Influence of Fluorescent Tags on IgG Partition in PEG–Salt Aqueous Two-Phase Systems for Rapid Screening Applications". Biotechnology Journal 14 8 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85069855349&partnerID=MN8TOARS.
    10.1002/biot.201800640
  26. Aires-Barros, R.; Azevedo, A.M.; Ferreira, G.N.M.. "Biochemical Engineering Science—Sustainable Processes and Economies". Biotechnology Journal 14 8 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85069840573&partnerID=MN8TOARS.
    10.1002/biot.201900276
  27. Nascimento, A.; Mullerpatan, A.; Azevedo, A.M.; Karande, P.; Cramer, S.. "Development of phage biopanning strategies to identify affinity peptide ligands for kappa light chain Fab fragments". Biotechnology Progress 35 6 (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85076329194&partnerID=MN8TOARS.
    10.1002/btpr.2884
  28. Capela, E.V.; Santiago, A.E.; Rufino, A.F.C.S.; Tavares, A.P.M.; Pereira, M.M.; Mohamadou, A.; Aires-Barros, M.R.; et al. "Sustainable strategies based on glycine-betaine analogue ionic liquids for the recovery of monoclonal antibodies from cell culture supernatants". Green Chemistry 21 20 (2019): 5671-5682. http://www.scopus.com/inward/record.url?eid=2-s2.0-85073594334&partnerID=MN8TOARS.
    10.1039/c9gc02733e
  29. Inês F Pinto; Denis R Santos; Catarina R F Caneira; Ruben R G Soares; Ana M Azevedo; Virginia Chu; João P Conde. "Optical biosensing in microfluidics using nanoporous microbeads and amorphous silicon thin-film photodiodes: quantitative analysis of molecular recognition and signal transduction". Journal of Micromechanics and Microengineering (2018): https://doi.org/10.1088/1361-6439/aac66c.
    10.1088/1361-6439/aac66c
  30. Sara A.S.L. Rosa; C.L. da Silva; M. Raquel Aires-Barros; A.C. Dias-Cabral; Ana M. Azevedo. "Thermodynamics of the adsorption of monoclonal antibodies in phenylboronate chromatography: Affinity versus multimodal interactions". Journal of Chromatography A (2018): https://doi.org/10.1016/j.chroma.2018.07.050.
    10.1016/j.chroma.2018.07.050
  31. Isabel Campos-Pinto; Emanuel V Capela; A Rita Silva-Santos; Miguel Arévalo Rodríguez; Poondi Rajesh Gavara; Marcelo Fernandez-Lahore; M Raquel Aires-Barros; Ana M Azevedo. "LYTAG-driven purification strategies for monoclonal antibodies using quaternary amine ligands as affinity matrices". Journal of Chemical Technology & Biotechnology (2018): https://doi.org/10.1002/jctb.5460.
    10.1002/jctb.5460
  32. André Nascimento; Inês F. Pinto; Virginia Chu; M. Raquel Aires-Barros; João P. Conde; Ana M. Azevedo. "Studies on the purification of antibody fragments". Separation and Purification Technology 195 (2018): 388-397. https://doi.org/10.1016/j.seppur.2017.12.033.
    10.1016/j.seppur.2017.12.033
  33. Ruben R. G. Soares; Denis R. Santos; Inês F. Pinto; Ana M. Azevedo; M. Raquel Aires-Barros; Virginia Chu; João P. Conde; et al. "Multiplexed microfluidic fluorescence immunoassay with photodiode array signal acquisition for sub-minute and point-of-need detection of mycotoxins". Lab on a Chip 18 11 (2018): 1569-1580. https://doi.org/10.1039/C8LC00259B.
    10.1039/C8LC00259B
  34. Jacinto, M.J.; Trabuco, J.R.C.; Vu, B.V.; Garvey, G.; Khodadady, M.; Azevedo, A.M.; Aires-Barros, M.R.; et al. "Enhancement of lateral flow assay performance by electromagnetic relocation of reporter particles". PLoS ONE 13 1 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85040368484&partnerID=MN8TOARS.
    10.1371/journal.pone.0186782
  35. Jacinto, M.J.; Patinha, D.J.S.; Marrucho, I.M.; Gonçalves, J.; Willson, R.C.; Azevedo, A.M.; Aires-Barros, M.R.. "M13 bacteriophage purification using poly(ionic liquids) as alternative separation matrices". Journal of Chromatography A 1532 (2018): 246-250. http://www.scopus.com/inward/record.url?eid=2-s2.0-85038622459&partnerID=MN8TOARS.
    10.1016/j.chroma.2017.12.005
  36. Pinto, I.F.; Santos, D.R.; Soares, R.R.G.; Aires-Barros, M.R.; Chu, V.; Azevedo, A.M.; Conde, J.P.. "A regenerable microfluidic device with integrated valves and thin-film photodiodes for rapid optimization of chromatography conditions". Sensors and Actuators, B: Chemical 255 (2018): 3636-3646. http://www.scopus.com/inward/record.url?eid=2-s2.0-85030467065&partnerID=MN8TOARS.
    10.1016/j.snb.2017.09.167
  37. Pinto, I.F.; Rosa, S.A.S.L.; Aires-Barros, M.R.; Azevedo, A.M.. "Exploring the use of heparin as a first capture step in the purification of monoclonal antibodies from cell culture supernatants". Biochemical Engineering Journal 104 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-84929646115&partnerID=MN8TOARS.
    10.1016/j.bej.2015.05.011
  38. Soares, R.R.G.; Santos, D.R.; Chu, V.; Azevedo, A.M.; Aires-Barros, M.R.; Conde, J.P.. "A point-of-use microfluidic device with integrated photodetector array for immunoassay multiplexing: Detection of a panel of mycotoxins in multiple samples". Biosensors and Bioelectronics 87 (2017): 823-831. http://www.scopus.com/inward/record.url?eid=2-s2.0-84988037419&partnerID=MN8TOARS.
    10.1016/j.bios.2016.09.041
  39. Campos-Pinto, I.; Espitia-Saloma, E.; Rosa, S.A.S.L.; Rito-Palomares, M.; Aguilar, O.; Arévalo-Rodríguez, M.; Aires-Barros, M.R.; Azevedo, A.M.. "Integration of cell harvest with affinity-enhanced purification of monoclonal antibodies using aqueous two-phase systems with a dual tag ligand". Separation and Purification Technology 173 (2017): 129-134. http://www.scopus.com/inward/record.url?eid=2-s2.0-84988410976&partnerID=MN8TOARS.
    10.1016/j.seppur.2016.09.017
  40. Almeida, A.; Rosa, A.M.M.; Azevedo, A.M.; Prazeres, D.M.F.. "A biomolecular recognition approach for the functionalization of cellulose with gold nanoparticles". Journal of Molecular Recognition (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85018824481&partnerID=MN8TOARS.
    10.1002/jmr.2634
  41. Silva-Santos, A.R.; Alves, C.P.A.; Prazeres, D.M.F.; Azevedo, A.M.. "A process for supercoiled plasmid DNA purification based on multimodal chromatography". Separation and Purification Technology 182 (2017): 94-100. http://www.scopus.com/inward/record.url?eid=2-s2.0-85016519878&partnerID=MN8TOARS.
    10.1016/j.seppur.2017.03.042
  42. Silva, D.F.C.; Azevedo, A.M.; Fernandes, P.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "Determination of partition coefficients of biomolecules in a microfluidic aqueous two phase system platform using fluorescence microscopy". Journal of Chromatography A 1487 (2017): 242-247. http://www.scopus.com/inward/record.url?eid=2-s2.0-85009737089&partnerID=MN8TOARS.
    10.1016/j.chroma.2016.12.036
  43. Pinto, I.F.; Caneira, C.R.F.; Soares, R.R.G.; Madaboosi, N.; Aires-Barros, M.R.; Conde, J.P.; Azevedo, A.M.; Chu, V.. "The application of microbeads to microfluidic systems for enhanced detection and purification of biomolecules". Methods 116 (2017): 112-124. http://www.scopus.com/inward/record.url?eid=2-s2.0-85007566334&partnerID=MN8TOARS.
    10.1016/j.ymeth.2016.12.005
  44. Ruivo, S.; Azevedo, A.M.; Prazeres, D.M.F.. "Colorimetric detection of D-dimer in a paper-based immunodetection device". Analytical Biochemistry 538 (2017): 5-12. http://www.scopus.com/inward/record.url?eid=2-s2.0-85029516236&partnerID=MN8TOARS.
    10.1016/j.ab.2017.09.009
  45. Grilo, A.L.; Mateus, M.; Aires-Barros, M.R.; Azevedo, A.M.. "Monoclonal Antibodies Production Platforms: An Opportunity Study of a Non-Protein-A Chromatographic Platform Based on Process Economics". Biotechnology Journal 12 12 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85038386195&partnerID=MN8TOARS.
    10.1002/biot.201700260
  46. Bras, E.J.S.; Soares, R.R.G.; Azevedo, A.M.; Fernandes, P.; Arévalo-Rodríguez, M.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "A multiplexed microfluidic toolbox for the rapid optimization of affinity-driven partition in aqueous two phase systems". Journal of Chromatography A 1515 (2017): 252-259. http://www.scopus.com/inward/record.url?eid=2-s2.0-85028061734&partnerID=MN8TOARS.
    10.1016/j.chroma.2017.07.094
  47. Soares, R.R.G.; Azevedo, A.M.; Fernandes, P.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "A simple method for point-of-need extraction, concentration and rapid multi-mycotoxin immunodetection in feeds using aqueous two-phase systems". Journal of Chromatography A (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85021980127&partnerID=MN8TOARS.
    10.1016/j.chroma.2017.07.004
  48. Aires-Barros, Raquel; Silva, Daniel; Azevedo, Ana; Fernandes, Pedro; Chu, Virginia; Conde, Joao Pedro. "Rapid determination of partition coefficients of biomolecules in a microfluidic ATPS platform using fluorescence microscopy". Abstracts of Papers of the American Chemical Society (2017): https://publons.com/wos-op/publon/28734194/.
  49. Silva-Santos, A.R.; Alves, C.P.A.; Prazeres, D.M.F.; Azevedo, A.M.. "Separation of plasmid DNA topoisomers by multimodal chromatography". Analytical Biochemistry 503 (2016): 68-70. http://www.scopus.com/inward/record.url?eid=2-s2.0-84964681172&partnerID=MN8TOARS.
    10.1016/j.ab.2016.03.012
  50. Grilo, A.L.; Aires-Barros, M.R.; Azevedo, A.M.. "Partitioning in Aqueous Two-Phase Systems: Fundamentals, Applications and Trends". Separation and Purification Reviews 45 1 (2016): 68-80. http://www.scopus.com/inward/record.url?eid=2-s2.0-84945903068&partnerID=MN8TOARS.
    10.1080/15422119.2014.983128
  51. Rosa, S.A.S.L.; Dos Santos, R.; Aires-Barros, M.R.; Azevedo, A.M.. "Phenylboronic acid chromatography provides a rapid, reproducible and easy scalable multimodal process for the capture of monoclonal antibodies". Separation and Purification Technology 160 (2016): 43-50. http://www.scopus.com/inward/record.url?eid=2-s2.0-84954511373&partnerID=MN8TOARS.
    10.1016/j.seppur.2016.01.002
  52. de Barros, D.P.C.; Campos, S.R.R.; Azevedo, A.M.; Baptista, A.M.; Aires-Barros, M.R.. "Predicting protein partition coefficients in aqueous two phase system". Journal of Chromatography A 1470 (2016): 50-58. http://www.scopus.com/inward/record.url?eid=2-s2.0-84995380022&partnerID=MN8TOARS.
    10.1016/j.chroma.2016.09.072
  53. Soares, R.R.G.; Silva, D.F.C.; Fernandes, P.; Azevedo, A.M.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "Miniaturization of aqueous two-phase extraction for biological applications: From micro-tubes to microchannels". Biotechnology Journal 11 12 (2016): 1498-1512. http://www.scopus.com/inward/record.url?eid=2-s2.0-84987642617&partnerID=MN8TOARS.
    10.1002/biot.201600356
  54. Pinto, I.F.; Soares, R.R.G.; Rosa, S.A.S.L.; Aires-Barros, M.R.; Chu, V.; Conde, J.P.; Azevedo, A.M.. "High-Throughput Nanoliter-Scale Analysis and Optimization of Multimodal Chromatography for the Capture of Monoclonal Antibodies". Analytical Chemistry 88 16 (2016): 7959-7967. http://www.scopus.com/inward/record.url?eid=2-s2.0-84983086201&partnerID=MN8TOARS.
    10.1021/acs.analchem.6b00781
  55. Alves, B.M.; Borlido, L.; Rosa, S.A.S.L.; Silva, M.F.F.; Aires-Barros, M.R.; Roque, A.C.A.; Azevedo, A.M.. "Purification of human antibodies from animal cell cultures using gum arabic coated magnetic particles". Journal of Chemical Technology and Biotechnology 90 5 (2015): 838-846. http://www.scopus.com/inward/record.url?eid=2-s2.0-84926115203&partnerID=MN8TOARS.
    10.1002/jctb.4378
  56. Jacinto, M.J.; Soares, R.R.G.; Azevedo, A.M.; Chu, V.; Tover, A.; Conde, J.P.; Aires-Barros, M.R.. "Optimization and miniaturization of aqueous two phase systems for the purification of recombinant human immunodeficiency virus-like particles from a CHO cell supernatant". Separation and Purification Technology 154 (2015): 27-35. http://www.scopus.com/inward/record.url?eid=2-s2.0-84942246622&partnerID=MN8TOARS.
    10.1016/j.seppur.2015.09.006
  57. Soares, R.R.G.; Azevedo, A.M.; Van Alstine, J.M.; Raquel Aires-Barros, M.. "Partitioning in aqueous two-phase systems: Analysis of strengths, weaknesses, opportunities and threats". Biotechnology Journal 10 8 (2015): 1158-1169. http://www.scopus.com/inward/record.url?eid=2-s2.0-84938985238&partnerID=MN8TOARS.
    10.1002/biot.201400532
  58. Taipa, M.A.; Azevedo, A.M.; Grilo, A.L.; Couto, P.T.; Ferreira, F.A.G.; Fortuna, A.R.M.; Pinto, I.F.; Santos, R.M.; Santos, S.B.. "Student Collaboration in a Series of Integrated Experiments To Study Enzyme Reactor Modeling with Immobilized Cell-Based Invertase". Journal of Chemical Education 92 7 (2015): 1238-1243. http://www.scopus.com/inward/record.url?eid=2-s2.0-84949474425&partnerID=MN8TOARS.
    10.1021/ed500842p
  59. Soares, R.R.G.; Novo, P.; Azevedo, A.M.; Fernandes, P.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "Aqueous two-phase systems for enhancing immunoassay sensitivity: Simultaneous concentration of mycotoxins and neutralization of matrix interference". Journal of Chromatography A 1361 (2014): 67-76. http://www.scopus.com/inward/record.url?eid=2-s2.0-84919691408&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.08.007
  60. Silva, M.F.F.; Fernandes-Platzgummer, A.; Aires-Barros, M.R.; Azevedo, A.M.. "Integrated purification of monoclonal antibodies directly from cell culture medium with aqueous two-phase systems". Separation and Purification Technology 132 (2014): 330-335. http://www.scopus.com/inward/record.url?eid=2-s2.0-84902685625&partnerID=MN8TOARS.
    10.1016/j.seppur.2014.05.041
  61. Bernardo, S.C.; Azevedo, A.M.; Aires-Barros, M.R.. "Integrated platforms for the clarification and capture of monoclonal antibodies,Plataformas integrales para la clarificaciony captura de anticuerpos monoclonales". Revista Mexicana de Ingeniera Qumica 13 2 (2014): 349-357. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904279317&partnerID=MN8TOARS.
  62. Rosa, A.M.M.; Louro, A.F.; Martins, S.A.M.; Inácio, J.; Azevedo, A.M.; Prazeres, D.M.F.. "Capture and detection of DNA hybrids on paper via the anchoring of antibodies with fusions of carbohydrate binding modules and ZZ-domains". Analytical Chemistry 86 9 (2014): 4340-4347. http://www.scopus.com/inward/record.url?eid=2-s2.0-84899814597&partnerID=MN8TOARS.
    10.1021/ac5001288
  63. Dhadge, V.L.; Hussain, A.; Azevedo, A.M.; Aires-Barros, R.; Roque, A.C.A.. "Boronic acid-modified magnetic materials for antibody purification". Journal of the Royal Society Interface 11 91 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84891893309&partnerID=MN8TOARS.
    10.1098/rsif.2013.0875
  64. Nascimento, A.; Rosa, S.A.S.L.; Mateus, M.; Azevedo, A.M.. "Polishing of monoclonal antibodies streams through convective flow devices". Separation and Purification Technology 132 (2014): 593-600. http://www.scopus.com/inward/record.url?eid=2-s2.0-84903717887&partnerID=MN8TOARS.
    10.1016/j.seppur.2014.06.005
  65. dos Santos, R.; Rosa, S.A.S.L.; Aires-Barros, M.R.; Tover, A.; Azevedo, A.M.. "Phenylboronic acid as a multi-modal ligand for the capture of monoclonal antibodies: Development and optimization of a washing step". Journal of Chromatography A 1355 (2014): 115-124. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904311566&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.06.001
  66. De Barros, D.P.C.; Campos, S.R.R.; Madeira, P.P.; Azevedo, A.M.; Baptista, A.M.; Aires-Barros, M.R.. "Modeling the partitioning of amino acids in aqueous two phase systems". Journal of Chromatography A 1329 (2014): 52-60. http://www.scopus.com/inward/record.url?eid=2-s2.0-84895141345&partnerID=MN8TOARS.
    10.1016/j.chroma.2013.12.015
  67. Dhadge, V.L.; Rosa, S.A.S.L.; Azevedo, A.; Aires-Barros, R.; Roque, A.C.A.. "Magnetic aqueous two phase fishing: A hybrid process technology for antibody purification". Journal of Chromatography A 1339 (2014): 59-64. http://www.scopus.com/inward/record.url?eid=2-s2.0-84897915765&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.02.069
  68. Carvalho, R.J.; Woo, J.; Aires-Barros, M.R.; Cramer, S.M.; Azevedo, A.M.. "Phenylboronate chromatography selectively separates glycoproteins through the manipulation of electrostatic, charge transfer, and cis-diol interactions". Biotechnology Journal 9 10 (2014): 1250-1258. http://www.scopus.com/inward/record.url?eid=2-s2.0-84908489196&partnerID=MN8TOARS.
    10.1002/biot.201400170
  69. Soares, R.R.G.; Novo, P.; Azevedo, A.M.; Fernandes, P.; Aires-Barros, M.R.; Chu, V.; Conde, J.P.. "On-chip sample preparation and analyte quantification using a microfluidic aqueous two-phase extraction coupled with an immunoassay". Lab on a Chip 14 21 (2014): 4284-4294. http://www.scopus.com/inward/record.url?eid=2-s2.0-84907611826&partnerID=MN8TOARS.
    10.1039/c4lc00695j
  70. Dhadge, V.L.; Morgado, P.I.; Freitas, F.; Reis, M.A.; Azevedo, A.; Aires-Barros, R.; Roque, A.C.A.. "An extracellular polymer at the interface of magnetic bioseparations". Journal of the Royal Society Interface 11 100 (2014): http://www.scopus.com/inward/record.url?eid=2-s2.0-84908151123&partnerID=MN8TOARS.
    10.1098/rsif.2014.0743
  71. Silva, D.F.C.; Azevedo, A.M.; Fernandes, P.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "Determination of aqueous two phase system binodal curves using a microfluidic device". Journal of Chromatography A 1370 (2014): 115-120. http://www.scopus.com/inward/record.url?eid=2-s2.0-84919435961&partnerID=MN8TOARS.
    10.1016/j.chroma.2014.10.035
  72. Borlido, L.; Moura, L.; Azevedo, A.M.; Roque, A.C.A.; Aires-Barros, M.R.; Farinha, J.P.S.. "Stimuli-Responsive magnetic nanoparticles for monoclonal antibody purification". Biotechnology Journal 8 6 (2013): 709-717. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878971074&partnerID=MN8TOARS.
    10.1002/biot.201200329
  73. Borlido, L.; Azevedo, A.M.; Roque, A.C.A.; Aires-Barros, M.R.. "Magnetic separations in biotechnology". Biotechnology Advances 31 8 (2013): 1374-1385. http://www.scopus.com/inward/record.url?eid=2-s2.0-84887995428&partnerID=MN8TOARS.
    10.1016/j.biotechadv.2013.05.009
  74. Rosa, P.A.J.; Azevedo, A.M.; Sommerfeld, S.; Mutter, M.; Bäcker, W.; Aires-Barros, M.R.. "Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process". Biotechnology Journal 8 3 (2013): 352-362. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874575364&partnerID=MN8TOARS.
    10.1002/biot.201200031
  75. Timopheev, A.A.; Azevedo, A.M.; Sobolev, N.A.; Brachwitz, K.; Lorenz, M.; Ziese, M.; Esquinazi, P.; Grundmann, M.. "Magnetic anisotropy of epitaxial zinc ferrite thin films grown by pulsed laser deposition". Thin Solid Films 527 (2013): 273-277. http://www.scopus.com/inward/record.url?eid=2-s2.0-84872594626&partnerID=MN8TOARS.
    10.1016/j.tsf.2012.12.028
  76. Rosa, P.A.J.; Azevedo, A.M.; Sommerfeld, S.; Bäcker, W.; Aires-Barros, M.R.. "Continuous aqueous two-phase extraction of human antibodies using a packed column". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 880 1 (2012): 148-156. http://www.scopus.com/inward/record.url?eid=2-s2.0-84855189061&partnerID=MN8TOARS.
    10.1016/j.jchromb.2011.11.034
  77. Nascimento, K.S.; Cunha, A.I.; Cavada, B.S.; Azevedo, A.M.; Aires-Barros, M.R.. "An overview of lectins purification strategies". Journal of Molecular Recognition 25 11 (2012): 527-541. http://www.scopus.com/inward/record.url?eid=2-s2.0-84868117352&partnerID=MN8TOARS.
    10.1002/jmr.2200
  78. Gomes, A.G.; Azevedo, A.M.; Aires-Barros, M.R.; Prazeres, D.M.F.. "Validation and scale-up of plasmid DNA purification by phenyl-boronic acid chromatography". Journal of Separation Science 35 22 (2012): 3190-3196. http://www.scopus.com/inward/record.url?eid=2-s2.0-84870021495&partnerID=MN8TOARS.
    10.1002/jssc.201200225
  79. de Barros, D.P.C.; Azevedo, A.M.; Cabral, J.M.S.; Fonseca, L.P.. "Optimization Of Flavor Esters Synthesis By Fusarium Solani Pisi Cutinase". Journal of Food Biochemistry 36 3 (2012): 275-284. http://www.scopus.com/inward/record.url?eid=2-s2.0-84861819057&partnerID=MN8TOARS.
    10.1111/j.1745-4514.2010.00535.x
  80. Borlido, L.; Azevedo, A.M.; Sousa, A.G.; Oliveira, P.H.; Roque, A.C.A.; Aires-Barros, M.R.. "Fishing human monoclonal antibodies from a CHO cell supernatant with boronic acid magnetic particles". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 903 (2012): 163-170. http://www.scopus.com/inward/record.url?eid=2-s2.0-84864951835&partnerID=MN8TOARS.
    10.1016/j.jchromb.2012.07.014
  81. Silva, D.F.C.; Azevedo, A.M.; Fernandes, P.; Chu, V.; Conde, J.P.; Aires-Barros, M.R.. "Design of a microfluidic platform for monoclonal antibody extraction using an aqueous two-phase system". Journal of Chromatography A 1249 (2012): 1-7. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863784327&partnerID=MN8TOARS.
    10.1016/j.chroma.2012.05.089
  82. Sousa, A.F.; Andrade, P.Z.; Pirzgalska, R.M.; Galhoz, T.M.; Azevedo, A.M.; da Silva, C.L.; Aires-Barros, M.R.; Cabral, J.M.S.. "A novel method for human hematopoietic stem/progenitor cell isolation from umbilical cord blood based on immunoaffinity aqueous two-phase partitioning". Biotechnology Letters 33 12 (2011): 2373-2377. http://www.scopus.com/inward/record.url?eid=2-s2.0-81155152440&partnerID=MN8TOARS.
    10.1007/s10529-011-0727-0
  83. Nascimento, K.S.; Yelo, S.; Cavada, B.S.; Azevedo, A.M.; Aires-Barros, M.R.. "Liquid-liquid equilibrium data for aqueous two-phase systems composed of ethylene oxide propylene oxide copolymers". Journal of Chemical and Engineering Data 56 2 (2011): 190-194. http://www.scopus.com/inward/record.url?eid=2-s2.0-79951521545&partnerID=MN8TOARS.
    10.1021/je1006532
  84. Rosa, P.A.J.; Azevedo, A.M.; Sommerfeld, S.; Bäcker, W.; Aires-Barros, M.R.. "Aqueous two-phase extraction as a platform in the biomanufacturing industry: Economical and environmental sustainability". Biotechnology Advances 29 6 (2011): 559-567. http://www.scopus.com/inward/record.url?eid=2-s2.0-80053444906&partnerID=MN8TOARS.
    10.1016/j.biotechadv.2011.03.006
  85. Gomes, A.G.; Azevedo, A.M.; Aires-Barros, M.R.; Prazeres, D.M.F.. "Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads". Journal of Chromatography A 1218 48 (2011): 8629-8637. http://www.scopus.com/inward/record.url?eid=2-s2.0-80455131119&partnerID=MN8TOARS.
    10.1016/j.chroma.2011.10.004
  86. Borlido, L.; Azevedo, A.M.; Roque, A.C.A.; Aires-Barros, M.R.. "Potential of boronic acid functionalized magnetic particles in the adsorption of human antibodies under mammalian cell culture conditions". Journal of Chromatography A 1218 43 (2011): 7821-7827. http://www.scopus.com/inward/record.url?eid=2-s2.0-80053571505&partnerID=MN8TOARS.
    10.1016/j.chroma.2011.08.084
  87. Gomes, A.G.; Azevedo, A.M.; Aires-Barros, M.R.; Prazeres, D.M.F.. "Clearance of host cell impurities from plasmid-containing lysates by boronate adsorption". Journal of Chromatography A 1217 15 (2010): 2262-2266. http://www.scopus.com/inward/record.url?eid=2-s2.0-77950690475&partnerID=MN8TOARS.
    10.1016/j.chroma.2010.02.015
  88. Azevedo, A.M.; Gomes, A.G.; Borlido, L.; Santos, I.F.S.; Prazeres, D.M.F.; Aires-Barros, M.R.. "Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography". Journal of Molecular Recognition 23 6 (2010): 569-576. http://www.scopus.com/inward/record.url?eid=2-s2.0-78049449358&partnerID=MN8TOARS.
    10.1002/jmr.1068
  89. Rosa, P.A.J.; Ferreira, I.F.; Azevedo, A.M.; Aires-Barros, M.R.. "Aqueous two-phase systems: A viable platform in the manufacturing of biopharmaceuticals". Journal of Chromatography A 1217 16 (2010): 2296-2305. http://www.scopus.com/inward/record.url?eid=2-s2.0-77950101404&partnerID=MN8TOARS.
    10.1016/j.chroma.2009.11.034
  90. Nascimento, K.S.; Azevedo, A.M.; Cavada, B.S.; Aires-Barros, M.R.. "Partitioning of canavalia brasiliensis lectin in polyethylene glycol - sodium citrate aqueous two-phase systems". Separation Science and Technology 45 15 (2010): 2180-2186. http://www.scopus.com/inward/record.url?eid=2-s2.0-77958617235&partnerID=MN8TOARS.
    10.1080/01496395.2010.507446
  91. Nascimento, K.S.; Rosa, P.A.J.; Cavada, B.S.; Azevedo, A.M.; Aires-Barros, M.R.. "Partitioning and recovery of Canavalia brasiliensis lectin by aqueous two-phase systems using design of experiments methodology". Separation and Purification Technology 75 1 (2010): 48-54. http://www.scopus.com/inward/record.url?eid=2-s2.0-77956434937&partnerID=MN8TOARS.
    10.1016/j.seppur.2010.07.008
  92. Borlido, L.; Azevedo, A.M.; Aires-Barros, M.R.. "Extraction of human IgG in thermo-responsive aqueous two-phase systems: Assessment of structural stability by circular dichroism". Separation Science and Technology 45 15 (2010): 2171-2179. http://www.scopus.com/inward/record.url?eid=2-s2.0-77958518181&partnerID=MN8TOARS.
    10.1080/01496395.2010.507441
  93. Rosa, P.A.J.; Azevedo, A.M.; Sommerfeld, S.; Mutter, M.; Aires-Barros, M.R.; Bäcker, W.. "Application of aqueous two-phase systems to antibody purification: A multi-stage approach". Journal of Biotechnology 139 4 (2009): 306-313. http://www.scopus.com/inward/record.url?eid=2-s2.0-60549115762&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2009.01.001
  94. Azevedo, A.M.; Rosa, P.A.J.; Ferreira, I.F.; Pisco, A.M.M.O.; de Vries, J.; Korporaal, R.; Visser, T.J.; Aires-Barros, M.R.. "Affinity-enhanced purification of human antibodies by aqueous two-phase extraction". Separation and Purification Technology 65 1 (2009): 31-39. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149299631&partnerID=MN8TOARS.
    10.1016/j.seppur.2008.03.006
  95. Azevedo, A.M.; Ferreira, G.N.M.; Aires-Barros, M.R.. "Advances in biopartitioning and purification". Separation and Purification Technology 65 1 (2009): 1-2. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149311056&partnerID=MN8TOARS.
    10.1016/j.seppur.2008.10.001
  96. Azevedo, A.M.; Gomes, A.G.; Rosa, P.A.J.; Ferreira, I.F.; Pisco, A.M.M.O.; Aires-Barros, M.R.. "Partitioning of human antibodies in polyethylene glycol-sodium citrate aqueous two-phase systems". Separation and Purification Technology 65 1 (2009): 14-21. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149316305&partnerID=MN8TOARS.
    10.1016/j.seppur.2007.12.010
  97. Azevedo, A.M.; Rosa, P.A.J.; Ferreira, I.F.; de Vries, J.; Visser, T.J.; Aires-Barros, M.R.. "Downstream processing of human antibodies integrating an extraction capture step and cation exchange chromatography". Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 877 1-2 (2009): 50-58. http://www.scopus.com/inward/record.url?eid=2-s2.0-57549117961&partnerID=MN8TOARS.
    10.1016/j.jchromb.2008.11.014
  98. Rosa, P.A.J.; Azevedo, A.M.; Ferreira, I.F.; Sommerfeld, S.; Bäcker, W.; Aires-Barros, M.R.. "Downstream processing of antibodies: Single-stage versus multi-stage aqueous two-phase extraction". Journal of Chromatography A 1216 50 (2009): 8741-8749. http://www.scopus.com/inward/record.url?eid=2-s2.0-67749110224&partnerID=MN8TOARS.
    10.1016/j.chroma.2009.02.024
  99. Azevedo, A.M.; Rosa, P.A.J.; Ferreira, I.F.; Aires-Barros, M.R.. "Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing". Trends in Biotechnology 27 4 (2009): 240-247. http://www.scopus.com/inward/record.url?eid=2-s2.0-62549139648&partnerID=MN8TOARS.
    10.1016/j.tibtech.2009.01.004
  100. Gomes, G.A.; Azevedo, A.M.; Aires-Barros, M.R.; Prazeres, D.M.F.. "Purification of plasmid DNA with aqueous two phase systems of PEG 600 and sodium citrate/ammonium sulfate". Separation and Purification Technology 65 1 (2009): 22-30. http://www.scopus.com/inward/record.url?eid=2-s2.0-58149289804&partnerID=MN8TOARS.
    10.1016/j.seppur.2008.01.026
  101. Azevedo, A.M.; Rosa, P.A.J.; Ferreira, I.F.; Aires-Barros, M.R.. "Integrated process for the purification of antibodies combining aqueous two-phase extraction, hydrophobic interaction chromatography and size-exclusion chromatography". Journal of Chromatography A 1213 2 (2008): 154-161. http://www.scopus.com/inward/record.url?eid=2-s2.0-56249124571&partnerID=MN8TOARS.
    10.1016/j.chroma.2008.09.115
  102. Ferreira, I.F.; Azevedo, A.M.; Rosa, P.A.J.; Aires-Barros, M.R.. "Purification of human immunoglobulin G by thermoseparating aqueous two-phase systems". Journal of Chromatography A 1195 1-2 (2008): 94-100. http://www.scopus.com/inward/record.url?eid=2-s2.0-44649137858&partnerID=MN8TOARS.
    10.1016/j.chroma.2008.04.077
  103. Azevedo, A.M.; Rosa, P.A.J.; Ferreira, I.F.; Aires-Barros, M.R.. "Optimisation of aqueous two-phase extraction of human antibodies". Journal of Biotechnology 132 2 (2007): 209-217. http://www.scopus.com/inward/record.url?eid=2-s2.0-35348995017&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2007.04.002
  104. Rosa, P.A.J.; Azevedo, A.M.; Aires-Barros, M.R.. "Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies". Journal of Chromatography A 1141 1 (2007): 50-60. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846011124&partnerID=MN8TOARS.
    10.1016/j.chroma.2006.11.075
  105. Rosa, P.A.J.; Azevedo, A.M.; Ferreira, I.F.; de Vries, J.; Korporaal, R.; Verhoef, H.J.; Visser, T.J.; Aires-Barros, M.R.. "Affinity partitioning of human antibodies in aqueous two-phase systems". Journal of Chromatography A 1162 1 SPEC. IS (2007): 103-113. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547229213&partnerID=MN8TOARS.
    10.1016/j.chroma.2007.03.067
  106. Azevedo, A.M.; Prazeres, D.M.F.; Cabral, J.M.S.; Fonseca, L.P.. "Ethanol biosensors based on alcohol oxidase". Biosensors and Bioelectronics 21 2 (2005): 235-247. http://www.scopus.com/inward/record.url?eid=2-s2.0-22044453261&partnerID=MN8TOARS.
    10.1016/j.bios.2004.09.030
  107. Vojinovic, V.; Azevedo, A.M.; Martins, V.C.B.; Cabral, J.M.S.; Gibson, T.D.; Fonseca, L.P.. "Assay of H2O2 by HRP catalysed co-oxidation of phenol-4-sulphonic acid and 4-aminoantipyrine: Characterisation and optimisation". Journal of Molecular Catalysis B: Enzymatic 28 2-3 (2004): 129-135. http://www.scopus.com/inward/record.url?eid=2-s2.0-1842685386&partnerID=MN8TOARS.
    10.1016/j.molcatb.2004.02.003
  108. Azevedo, A.M.; Cabral, J.M.S.; Prazeres, D.M.F.; Gibson, T.D.; Fonseca, L.P.. "Thermal and operational stabilities of Hansenula polymorpha alcohol oxidase". Journal of Molecular Catalysis B: Enzymatic 27 1 (2004): 37-45. http://www.scopus.com/inward/record.url?eid=2-s2.0-0346095156&partnerID=MN8TOARS.
    10.1016/j.molcatb.2003.09.001
  109. Azevedo, A.M.; Vojinovic, V.; Cabral, J.M.S.; Gibson, T.D.; Fonseca, L.P.. "Operational stability of immobilised horseradish peroxidase in mini-packed bed bioreactors". Journal of Molecular Catalysis B: Enzymatic 28 2-3 (2004): 121-128. http://www.scopus.com/inward/record.url?eid=2-s2.0-1842786129&partnerID=MN8TOARS.
    10.1016/j.molcatb.2004.02.005
  110. Azevedo, A.M.; Cabral, J.M.S.; Gibson, T.D.; Fonseca, L.P.. "Operation and performance of analytical packed-bed reactors with an immobilised alcohol oxidase". Journal of Molecular Catalysis B: Enzymatic 28 2-3 (2004): 45-53. http://www.scopus.com/inward/record.url?eid=2-s2.0-1842786138&partnerID=MN8TOARS.
    10.1016/j.molcatb.2004.01.001
  111. Azevedo, A.M.; Prazeres, D.M.F.; Cabral, J.M.S.; Fonseca, L.P.. "Stability of free and immobilised peroxidase in aqueous-organic solvents mixtures". Journal of Molecular Catalysis - B Enzymatic 15 4-6 (2001): 147-153. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035501590&partnerID=MN8TOARS.
    10.1016/S1381-1177(01)00017-0
  112. Azevedo, A.M.; Fonseca, L.P.; Graham, D.L.; Cabral, J.M.S.; Prazeres, D.M.F.. "Behaviour of horseradish peroxidase in AOT reversed micelles". Biocatalysis and Biotransformation 19 3 (2001): 213-233. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034925825&partnerID=MN8TOARS.
  113. Azevedo, A.M.; Fonseca, L.P.; Prazeres, D.M.F.. "Kinetic and stability studies of penicillin acylase in reversed micelles". Biocatalysis and Biotransformation 17 6 (2000): 401-415. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033963093&partnerID=MN8TOARS.
  114. Azevedo, A.M.; Fonseca, L.P.; Prazeres, D.M.F.. "Stability and stabilisation of penicillin acylase". Journal of Chemical Technology and Biotechnology 74 11 (1999): 1110-1116. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032714181&partnerID=MN8TOARS.
    10.1002/(SICI)1097-4660(199911)74:11<1110::AID-JCTB149>3.0.CO;2-B

Intellectual property

Patent
  1. Azevedo, A.M.. 2010. "Verfahren zur reinigung therapeutischer proteine mittels mehrstufiger extraktion".
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2021/05/17 Screening of optimal conditions for phage purification using a chromatography microfluidic device 15th International Symposium on Biochromatography and Nanoseparations | SBCN 2021
(France)
2020/09 Continuous extraction platform using aqueous two-phase systems for the integrated purification of biological products” European Symposium on Biochemical Engineering Science | ESBES 2020 – virtual meeting
European Society on Biochemical Engineering Science
2019/11 Continuous Extraction of Monoclonal Antibodies using ATPS: From macro to micro-scale European Summit of Industrial Biotechnology | esib2019
Austrian Centre of Industrial Biotechnology | acib (Graz, Austria)
2019/11 Microfluidic-based strategies to fasten chromatographic process development 20th International Conference on Biopartitioning and Purification | BPP2018
Sociedade Brasileira de Microbiologia | SBM (Guarujá, Brazil)
2018/09 Miniaturization of chromatography in a lab-on-a-chip platform for screening of binding and elution conditions 12th European Symposium on Biochemical Engineering Science | ESBES-12
Instituto Superior Técnico (Lisbon, Portugal)
2017/09 Continuous Extraction Strategies for Monoclonal Antibodies: Form Macro- to Micro-Scale Integrated Continuous Biomanufacturing III - ICB III
Engineeing Conferences International | ECI (Cascais, Portugal)
2017/04 A Microfluidic Approach Towards High-Throughput Screening of Chromatographic Conditions 253rd American Chemical Society National Meeting & Exposition
American Chemical Society (San Francisco, United States)
2017/03 LYTAG-driven purification strategies as a key to integrate and intensify the downstream processing of monoclonal antibodies Separations Technology IX: New Frontiers in Media, Techniques, and Technologies
Engineering Conferences International | ECI (Albufeira, Portugal)
2017/02 A Microfluidic Approach Towards High-Throughput Screening of Chromatographic Conditions 10th HIC/RPC Hydrophobic Bioprocessing Conference
(Scottsdale, Arizona, United States)
2016/11 LYTAG-driven polishing strategies for monoclonal antibodies extracts using quaternary amine as affinity matrices International Symposium on the Separation of Proteins, Peptides and Polynucleotides | ISPPP 2016
(Salzburg, Austria)
2016/01 Phenylboronic acid Chromatography: A multimodal approach for the capture of monoclonal antibodies XVI COLACRO – Latin-American Congress on Chromatography
(Lisboa, Portugal)
2015/03 An alternative multi-modal process for the capture of monoclonal antibodies based on phenylboronic acid chromatography 249th American Chemical Society National Meeting & Exposition
American Chemical Society | ACS (Denver, Colorado, United States)
2014/09 Phenylboronic acid chromatography: Validation and scale-up of an alternative multi-modal process for the capture of monoclonal antibodies 34th International Symposium on the Separation of Proteins, Peptides and Polynucleotides | ISPPP2014
(Würzburg, Germany)
2014/09 A novel capture step for monoclonal antibodies from mammalian cell cultures: Phenyl boronate as an alternative ligand to protein A 10th European Symposium on Biochemical Engineering Science | ESBES-10
(Lille, France)
2013/10 A novel approach for mAbs bioprocessing: Clarification and capture by aqueous two-phase extraction 17th International Conference on Biopartitioning and Purification | BPP2013
(New Port, Rhode Island, United States)
2011/09 Polishing strategies for the purification of Canavalia brasiliensis lectin (ConBr) two-phase extracts: Ultrafiltration vs. multistage extraction 16th International Conference on Biopartitioning and Purification | BPP2011
(Puerto Vallarta, Mexico)
2009 Continuous aqueous two-phase extraction process for the purification of antibodies: Performance and economical feasibility 15th International Conference on Biopartitioning and Purification | BPP2009
Brunnel University (Uxbridge, United Kingdom)
2008 Alternative process for the purification of therapeutic antibodies comprising aqueous two-phase extraction 28th International Symposium on the Separation of Proteins, Peptides and Polynucleotides | ISPPP2008
(Baden-Baden, Germany)
2008 Affinity-enhanced partitioning of human antibodies in aqueous two-phase systems 7th European Symposium on Biochemical Engineering Science | ESBES-7
(Faro, Portugal)
2006 Optimisation of aqueous two-phase extraction of human antibodies” 6th European Symposium on Biochemical Engineering Science | ESBES-6
(Austria)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2020/02/01 - Current Designing continuous manufacturing processes for mRNA-based therapeutics
Supervisor of Sara Sousa Rosa
 Doutoramento em Biotecnologia e Biociências (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/09/01 - Current Continuous downstream strategy for industrial enzymes
Supervisor of Diogo Filipe Frazão Ferreira Faria
 PhD in Biotechnology and Biosciences (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/09/01 - Current Purification of monoclonal antibodies (Immunoglobulin G, IgG) using aqueous biphasic systems composed of bio-based ionic liquids
Co-supervisor
 PhD in Chemical Engineering (PhD)
Universidade de Aveiro, Portugal
2016/10/01 - Current Design and Optimization of a Scalable Manufacturing Process for Bacteriophages
Supervisor of Tatiana Vieira Arriaga
 PhD in Biotechnology and Biosciences (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/03/01 - 2025/02/28 Design of a manufacturing platform for recombinant antibody fragments for therapeutics or diagnostic purposes
Supervisor of Sara dos Santos Viveiros
 Biotechnology and Biosciences (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015/09/01 - 2020/04 Development of a novel purification platform for Fab antibody fragments based on affinity peptides and multimodal ligands
Supervisor of André Nunes dos Santos Nascimento
 PhD in Biotechnology and Biosciences (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015/02/01 - 2019/09/09 A phenylboronate chromatography based approach as an effective alternative for the downstream processing of biomolecules
Supervisor of Sara Alexandra da Silva Lourenço Rosa
 PhD in Biotechnology and Biosciences (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014 - 2019 Integrative platforms for downstream processing and detection of viral particles
Co-supervisor
 PhD in Bioengineeering (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014/02/01 - 2018/09/24 Screening of multimodal ligands for the capture and polishing of antibody streams: a microfluidic approach for rapid optimization of chromatography operating conditions
Supervisor of Inês Fernandes Pinto
 PhD in Biotechnology and Biosciences (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016 - 2016 Novel strategies for the purification of Fab fragments based on aqueous two-phase systems
Supervisor
 MSc in Molecular Nano- and Bio-photonics for Telecommunications and Biotechnologies (Monabiphot) (Master)
École Normale Supérieure Paris-Saclay, France
2015 - 2015 Multimodal Chromatography for the Capture of Monoclonal Antibodies
Supervisor of Yün Zhai
 MSc in Bioengineering (Master)
Karlsruher Institut für Technologie, Germany
2009 - 2013 A novel approach for the capture of monoclonal antibodies using continuous bed chromatography
Supervisor
 PhD in Bioengineering (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2008 - 2012 Affinity purification of monoclonal antibodies using magnetic nano- and micro-particles supported in aqueous-two phase systems
Co-supervisor
 PhD in Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2006 - 2010 Purification of lectins by aqueous two-phase systems
Co-supervisor
 PhD in Biotechnology (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2022/10/09 - 2022/10/13 Integrated Continuous Biomanufacturing | ICB V (ECI Engineering Conferences International), Stiges, Spain (2022/10/09 - 2022/10/13)
Conference (Co-organisor)
 Engineering Conferences International, United States
2022/09/25 - 2022/09/28 21th International Conference on Biopartitioning and Purification | BPP 2022, Aveiro, Portugal (2022/09/25 - 2022/09/28)
Conference (Co-organisor)
2021/11/07 - 2021/11/10 40th International Symposium on the Separation of Proteins, Peptides & Polynucleotides (2021/11/07 - 2021/11/10)
Conference (Co-organisor)
2018/09/09 - 2018/09/12 11th symposium of the European Society of Biochemical Engineering Sciences | ESBES 2018 (2018/09/09 - 2018/09/12)
Conference (Co-organisor)
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/11/22 - 2017/11/24 1st Workshop on Anything but Conventional Chromatography | ABC2 (2017/11/22 - 2017/11/24)
Workshop (Co-organisor)
2012/05/02 - 2012/05/04 9th International Conference on Protein Stabilisation | ProStab2012 (2012/05/02 - 2012/05/04)
Conference (Co-organisor)
 Universidade de Lisboa Instituto Superior Técnico, Portugal
2011/06/16 - 2011/06/19 19th Biennial Meeting of the International Society of the Molecular Recognition | Affinity 2011 (2011/06/16 - 2011/06/19)
Conference (Member of the Organising Committee)
2007/06/17 - 2007/06/20 14th International Conference on Biopartitioning and Purification | BPP2007 (2007/06/17 - 2007/06/20)
Conference (Member of the Organising Committee)
 Universidade de Lisboa Instituto Superior Técnico, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2019/03/18 - 2019/03/21 Bioprocessing Summit Europe
Conference
 Bioprocessing Summit Europe
Cambridge Healthtech Institute, United States

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2019/12/13 Advancing purification of biotherapeutic particles
(Thesis) Arguer
 Mafalda Sofia Gameiro Moleirinho (PhD)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2019/11/27 Design and functionalization of alumina monoliths for protein purification by chromatography
(Thesis) Main arguer
 Nayan Nagesh Nayak (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/07/01 Biophysical study of therapeutic antibody adsorption in affinity chromatography
(Thesis) Main arguer
 Gonçalo Fradique Lopes da Silva (PhD)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2019/03 Probing the interplay between calcitonins and islet amyloid polypeptides with membranes using advanced fluorescence methodologies
Thesis Member
 Joana Catarina Ribeiro Ricardo (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019/02/15 Construction of an immunosensor for human cytomegalovirus infection diagnosis
(Thesis) Main arguer
 Filipa Andreia Valez Pires (PhD)
Universidade da Beira Interior Centro de Investigação em Ciências da Saúde, Portugal
2019 Molecular recognition strategies combined to mesofluidics for solid-phase renewable assays
(Thesis) Main arguer
 Inês Isabel Barros Moreira Ramos (PhD)
Universidade do Porto Faculdade de Farmácia, Portugal
2018/10/25 Model based process development for biopharmaceuticals
(Thesis) Main arguer
 Silvia M. Pirrung (PhD)
Technische Universiteit Delft, Netherlands
2018/09/21 Development of purification platforms for immunoglobulin Y using aqueous biphasic systems
(Thesis) Main arguer
 • Ana Mafalda Rodrigues Almeida Rocha (PhD)
Universidade de Aveiro, Portugal
2018/09 Novel anti-carbohydrate antibodies and T cell based technologies: a combined approach to cancer immunotherapy
Thesis Member
 Liliana Raquel Rodrigues Loureiro (PhD)
2018/04/23 The usage of solubility curves and self-interaction chromatography for prediction of protein crystallization
(Thesis) Main arguer
 Susanne Zich (PhD)
Universität für Bodenkultur Wien, Austria
2016/07 Magnetic purification of antibodies
(Thesis) Main arguer
 Vijaykumar Laxmanrao Dhadge (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2015/12 Beta-Glucans from Basidiomycete strains: Isolation, purification, characterization and production of monoclonal antibodies
Thesis Member
 Magda Sofia Soares de Carvalho Cardoso Nobre Semedo (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal

Conference scientific committee

Conference name Conference host
2022/07/10 - 2022/07/15 Recovery of Biological Products XIX Conference: Past, Present and Future | RXIX Rome, Italy
2021/11/07 - 2021/11/10 40th International Symposium on the Separation of Proteins, Peptides & Polynucleotides Porto, Portugal
2019/11/11 - 2019/11/13 20th International Conference on Biopartitioning and Purification | BPP2019 Guarujá, Brazil
2018/09/09 - 2018/09/12 11th symposium of the European Society of Biochemical Engineering Sciences | ESBES 2018 Lisbon, Portugal
2017/11/22 - 2017/11/24 1st Anything but Conventional Chromatography | ABC2 Workshop Lisbon, Portugal

Evaluation committee

Activity description
Role 		Institution / Organization Funding entity
2015 - 2019 FCT-PhD Program Biotechnology and Biosciences | BIOTECnico
Evaluator
 Universidade de Lisboa Instituto Superior Técnico, Portugal Fundação para a Ciência e a Tecnologia

Journal scientific committee

Journal title (ISSN) Publisher
2015 - Current Journal of Chromatography A (0021-9673) Elsevier
2014 - Current Biotechnology Journal (1860-7314) WILEY-VCH Verlag GmbH & Co. KGaA
2013 - Current Journal of Chemical Technology & Biotechnology (1097-4660) Wiley (John Wiley & Sons)
2012 - Current Separation and Purification Technology (1383-5866) Elsevier
Distinctions

Award

2019 UL/CGD Scientific award in the area of Biology, Biological Engineering, Biochemistry and Biotechnology
2018 ESBES Award at ESBES 2018
2018 IST “Excellence in Teaching” Award
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 Outstanding poster award at ICB III
Engineering Conferences International, United States
2016 Best poster award at ISPPP2016
2016 Best poster award at microTAS 2016
2016 Best poster award at Eurosensors XXX
2011 Best Oral communication at Affinity 2011
2008 Best poster prize at ISPPP2008
2008 Poster prize at SPICA 2008

Other distinction

2007 Top cited article
Elsevier BV, Netherlands