???global.info.a_carregar???
Maria João Romão got her PhD in Chemistry in 1989 at Instituto Superior Técnico, Lisbon and was a AvHumboldt post-doctoral fellow working with the Nobel Laureate Prof. Robert Huber at the Max Planck Institute for Biochemistry, Martinsried, Germany. She got her Habilitation in Biochemistry at Faculdade de Ciências e Tecnologia, NOVA University of Lisbon, in 2001 and, since 2012, she is Full Professor of Biochemistry and Structural Biology at the same University. She is the founding Director of the Research Unit on Applied Biomolecular Sciences, UCIBIO (from 2014 to 2023). Her research group on Macromolecular Crystallography (http://sites.fct.unl.pt/xtal) has several areas of interest focused on molybdoenzymes (aldehyde oxidases, arsenite oxidases and formate dehydrogenases) and their enzymatic mechanisms, glycan-binding and cellulosomal proteins and methods for protein crystallization. She has supervised/co-supervised 17 PhD students and is the Principal investigator in 7 project(s) and Researcher in 25 project(s). Since 2022 she is the local coordinator of the Horizon Europe ERA-Chair project CryoEM@NOVA for the implementation of the first CryoEM lab at NOVA. In the period 2012-2022 she was the Portuguese Delegate to the European Synchrotron Radiation Laboratory (ESRF) and the Portuguese observer at the Scientific Advisory Committee of ALBA Synchrotron. Since 2017 is Co-Editor of Acta Crystallographica F. She is co-author of over 160 SCI publications, 10 book chapters and her work has received over 6600 citations, h-index= 45
Identification

Personal identification

Full name
Maria Joao Romão

Citation names

  • Romão, Maria
  • Maria Joao Romao

Author identifiers

Ciência ID
3315-C689-4557
ORCID iD
0000-0002-3004-0543
Google Scholar ID
CRCXDwYAAAAJ
Researcher Id
A-4115-2013
Scopus Author Id
7004694870

Email addresses

  • mjr@fct.unl.pt (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Biochemistry

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
German Advanced (C1) Intermediate (B1) Intermediate (B1) Advanced (C1)
French Intermediate (B1) Advanced (C1) Intermediate (B1) Intermediate (B1)
Education
Degree Classification
2001/05/10
Concluded
Biochemistry (Título de Agregado)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"n.a." (THESIS/DISSERTATION)
n.a.
1989
Concluded
Chemistry (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
""Reacções de cetonas com carbenóides de lítio: estudos estruturais e mecanísticos"." (THESIS/DISSERTATION)
Distinção e Louvor
1978
Concluded
Engenharia Quimica (Licenciatura)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"n.a." (THESIS/DISSERTATION)
16
Affiliation

Teaching in Higher Education

Category
Host institution
Employer
2012/06/01 - Current Full Professor (University Teacher) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
1998/09/01 - 2012/05/30 Associate Professor (University Teacher) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
1989/01/26 - 1998/09/02 Assistant Professor (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal
1979/01/02 - 1989/01/25 Assistant (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal

Positions / Appointments

Category
Host institution
Employer
2014 - 2020 Conselho científico/técnico-científico ou orgão correspondente Universidade Nova de Lisboa, Portugal
2013/02/25 - 2017/11/14 President of the Chemistry Department Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Others

Category
Host institution
Employer
1998/09/02 - Current Group Leader - Structural Biology (Xtal@FCT-NOVA) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2014/12/31 - 2023/02/01 Founding Director of the Research Unit UCIBIO Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Universidade Nova de Lisboa, Portugal
1992/02/01 - 1998/09/01 Group Leader - Macromolecular Crystallography Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Projects

Grant

Designation Funders
2021/01/01 - Current Understanding enzymatic CO2 reduction for enhanced hybrid catalysts
PTDC/BII-BBF/2050/2020
Co-Principal Investigator (Co-PI)
Fundação para a Ciência e a Tecnologia, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2022/11/11 - 2027/12/31 CryoEM@NOVA: a cryo electron microscopy hub enabling drug discovery
Supervisor
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

UCIBIO- Unidade de Ciências Biomoleculares Aplicadas, Portugal
Horizon 2020
Ongoing
2023/01/01 - 2024/06/30 Novos biossensores para avaliação rápida do metabolismo oral do azoto: um estudo transversal
2022.04940.PTDC
Egas Moniz - Cooperativa de Ensino Superior Crl, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2022/01 - 2023/06 Structural and biochemical characterization of PHA synthases towards novel Polyhydroxyalkanoates
EXPL/BIA-BQM/1300/2021
EXPL/BIA-BQM/1300/2021
Associação para a Inovação e Desenvolvimento da FCT, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/10/01 - 2021/09/30 Detoxification of Hydrogen Peroxide by Pathogenic Bacteria - E.coli Tri-haem peroxidase as a model (Co-PI)
PTDC/BIA-BQM/29442/2017
Researcher
Associação para a Inovação e Desenvolvimento da FCT, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/10/01 - 2021/09/30 Fighting Staphylococcus aureus - Peptidoglycan amidation as a new target
PTDC/BIA-MIC/31645/2017
Researcher
Associação para a Inovação e Desenvolvimento da FCT, Portugal

Universidade Nova de Lisboa, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/09/17 - 2021/09/16 Bacterial ABC-type Multitask Energizers: one ATPase fits all? (Co-PI)
PTDC/BIA-MIC/30696/2017
Researcher
Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/09/01 - 2021/08/31 Glycan-human microbiome interaction: glycan diversity and recognition in the human gut with impact on health and nutrition
PTDC/BIA-MIB/31730/2017
Researcher
Associação para a Inovação e Desenvolvimento da FCT, Portugal

Universidade de Aveiro, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2016/04/01 - 2019/04/01 Targeting p53 family proteins: on the route to new anticancer agents
PTDC/DTP-FTO/1981/2014
Researcher
Universidade do Minho, Portugal

Universidade de Coimbra, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia
Concluded
2016/01 - 2019/01 Aldehyde Oxidases in Drug Metabolism and Biocatalysis: Searching for the Substrate Specificity and Catalytic Mechanism<br>
PTDC/BBB-BEP/1185/2014
Principal investigator
Associação para a Inovação e Desenvolvimento da FCT, Portugal

UCIBIO- Unidade de Ciências Biomoleculares Aplicadas, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia
Concluded
2016/01 - 2018/12 CtIncipedia – A multidisciplinary approach to study Chlamydia trachomatis inclusion membrane (Inc) proteins
PTDC/IMI-MIC/1300/2014
Researcher
Associação para a Inovação e Desenvolvimento da FCT, Portugal

REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia
Ongoing
2016/01 - 2018/12 An integrative structural biology approach to characterise the protein-carbohydrate microbial recognition (PI from Romao group)
PTDC/BBB-BEP/0869/2014
Researcher
Associação para a Inovação e Desenvolvimento da FCT, Portugal

REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia
Concluded
2015/12 - 2018/11 Reduction of CO2 for sustainable biofuel production
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2013/07/01 - 2017/03/31 Modern Structural Biology: Resources for the advancement of in-house X-ray Crystallography (PI from Romao group)
RECI/BBB-BEP/0124/2012
Researcher
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia
Concluded
2013/07/01 - 2015/12/31 Amidation of the peptidoglycan of Gram-positive bacteria: an unexplored potential target for antibiotics
PTDC/BIA-MIC/3195/2012
Researcher
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2013/01/01 - 2015/12/31 ENZYMATIC CATALYSIS - seeking a global picture
EXCL/QEQ-COM/0394/2012
Researcher
Universidade do Porto Instituto de Ciências e Tecnologias Agrárias e Agro-Alimentares, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2013/04 - 2015/04 Ionic liquid-based systems for protein crystallization
PTDC/BBB-BEP/3058/2012
Researcher
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia, I.P.

Fundação para a Ciência e a Tecnologia
Concluded
2012/02 - 2015/01 Mammalian and Bacterial Aldehyde Oxidases: Structure, mechanism and insights into cofactor binding and protein maturation (PI from Romao group)
PTDC/BIA-PRO/118377/2010
Researcher
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal

Universidade do Porto Faculdade de Ciências, Portugal
Fundação para a Ciência e a Tecnologia, I.P.

Fundação para a Ciência e a Tecnologia
Concluded
2010/02 - 2014/01 Engineered biomimetics for large-scale enrichment of phosphoproteins
PTDC/EBB-BIO/102163/2008
Researcher
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
Fundação para a Ciência e a Tecnologia, I.P.

Fundação para a Ciência e a Tecnologia
Concluded
2010/01/01 - 2013/06/30 Structure and function of novel Glycoside Hydrolases and Carbohydrate-Binding Modules of Clostridium thermocellum cellulosome
PTDC/BIA-PRO/103980/2008
Researcher
Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2010/01 - 2013/06 The Cellulosome assembly: structure, function and specificity of new cellulases and supramolecular complexes (PI from Romao group)
PTDC/QUI-BIQ/100359/2008
Researcher
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
Fundação para a Ciência e a Tecnologia, I.P.

Fundação para a Ciência e a Tecnologia
Concluded
2009/01 - 2012/09 Production of a stable form of human phenylalanine hydroxylase: towards the 3D structure determination.
PTDC/QUI/64023/2006
Researcher
Universidade de Lisboa Faculdade de Farmácia, Portugal
Fundação para a Ciência e a Tecnologia, I.P.

Fundação para a Ciência e a Tecnologia
Concluded
2009/01/01 - 2011/12/31 Structure and function of novel carbohydrate-binding modules and cohesin-dockerin protein complexes from Clostridium thermocellum
PTDC/BIA-PRO/69732/2006
Researcher
Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2008/04/01 - 2011/12/31 Structural characterization of mRNA localization mechanisms (PI from Romao group)
PTDC/BIA-PRO/80486/2006
Researcher
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2008/01 - 2011/09 A combined approach of X-ray Crystallography, NMR and Computational Chemistry for the study of the molecular interactions that define the ligand specificity in cellulosomal Carbohydrate Binding Modules (PI from Romao group)
PTDC/QUI/68286/2006
Researcher
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal

Universidade do Porto Instituto de Ciências e Tecnologias Agrárias e Agro-Alimentares, Portugal
Fundação para a Ciência e a Tecnologia, I.P.

Fundação para a Ciência e a Tecnologia
Concluded
2008/01/01 - 2011/07/31 Structural and functional studies of the SOUL/HBP family of heme binding proteins
PTDC/QUI/64203/2006
Researcher
Universidade de Aveiro, Portugal

Universidade de Aveiro CICECO, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2008/01/02 - 2011/06/30 Xanthine Oxidase-Related Enzymes: Structure, Function and Specificity
PTDC/QUI/64733/2006
Principal investigator
Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal
Fundação para a Ciência e a Tecnologia

Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2007/01 - 2009/06 New molybdo and copper cofactors in proteins isolated from sulfate reducers
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
2007/01 - 2009/04 Molecular determinants of ligand specificity in carbohydrate-binding modules and cohesin-dockerin complexes (PI from Romao group)
Researcher
Fundação para a Ciência e a Tecnologia, I.P.
2007/01 - 2008/11 Structural and Functional Characterization of Nitrate Reductases and Formate Dehydrogenases
Principal investigator
Fundação para a Ciência e a Tecnologia, I.P.
2000/04/01 - 2004/03/31 HPRN-CT-1999-00084, The mechanism, specificity and inhibition of enzymes belonging to the xanthine oxidase family : medical and industrial applications (8 EU partners; PT coordinator)
Principal investigator
Universidade Nova de Lisboa, Portugal
European Commission
Concluded
2000 - 2004 A crystallographic contribution to the understanding of the structure and function of Mo-enzymes
POCTI/1999/BME/35078
POCTI/1999/BME/35078
Principal investigator
Universidade Nova de Lisboa, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
1999 - 2001 Molecular Biology and Structural Studies in Metalloproteins
PRAXIS/C/BIA/11089/1998
Principal investigator
Universidade Nova de Lisboa, Portugal
Fundação para a Ciência e a Tecnologia, I.P.
Concluded
1996/11 - 1999/10 BIO4-CT96-0413 “Construction of Novel Redox Proteins from modular building blocks” (5 EU Partners; PT coordinador)
Principal investigator
Universidade Nova de Lisboa Departamento de Química, Portugal
European Commission
Concluded
1993 - 1996 ID: CHRX930243 Pteridines: biosynthesis, regulation and pharmacology (7 EU participants; PT coordinator)
Principal investigator
Universidade Nova de Lisboa, Portugal
European Commission
Concluded

Contract

Designation Funders
2023/03/01 - 2026/02/28 Descoberta de proteínas do microbioma humano selectivas para O-glicanos ¿ ferramentas para novas abordagens na investigação em cancro
2022.06104.PTDC
Associação para a Inovação e Desenvolvimento da FCT, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2021/01/01 - 2025/12/31 Institute for Health and Bioeconomy
LA/P/0140/2020
Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Universidade NOVA de Lisboa, Portugal

INESC Microsistemas e Nanotecnologias, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2020/01/01 - 2023/12/31 Applied Molecular Biosciences Unit
UIDB/04378/2020
Universidade NOVA de Lisboa, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal

Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal

Universidade do Porto, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2020/01/01 - 2023/12/31 Applied Molecular Biosciences Unit
UIDP/04378/2020
Universidade NOVA de Lisboa, Portugal

Unidade de Ciências Biomoleculares Aplicadas, Portugal

Rede de Química e Tecnologia Laboratório Associado para a Química Verde, Portugal

Associação para a Inovação e Desenvolvimento da FCT, Portugal

Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal

Universidade do Porto, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book
  1. Brás, J.L.A.; Carvalho, A.L.; Viegas, A.; Najmudin, S.; Alves, V.D.; Prates, J.A.M.; Ferreira, L.M.A.; et al. Escherichia coli expression, purification, crystallization, and structure determination of bacterial cohesin-dockerin complexes. 2012.
    10.1016/B978-0-12-415931-0.00021-5
  2. Romão, M.J.; Archer, M.. Structural versatility of proteins containing rubredoxin-type centers. 2008.
    10.1002/9783527613700.ch20
Book chapter
  1. Oliveira, Ana Rita; Mota, Cristiano; Romão, Maria; Inês Cardoso Pereira. "The W/SeCys-FdhAB formate dehydrogenase from Desulfovibrio vulgaris Hildenborough". 1-12. Wiley, 2022.
    10.1002/9781119951438.eibc2812
  2. Cristiano De Sousa Mota; Teresa Sacadura Santos-Silva; Mineko Terao; Enrico Garattini; Maria João Romão; Silke Leimkühler. "Aldehyde Oxidases as Enzymes in Phase I Drug Metabolism". edited by Grunwald, {Peter. Routledge Taylor & Francis Group, 2019.
    10.1201/9780429295034-13
  3. Maria João Romão; Teresa Sacadura Santos-Silva; Ana Luísa Carvalho; Eurico J Cabrita; Filipa Margarida Barradas Morais Marcelo. "Structural elucidation of macromolecules". 30-91. Bentham Science Publishers Ltd., 2018.
    10.2174/97816810870921180101
  4. Maria João Romão. "The role of molybdenum in biology". 507-510. JOHN LIBBEY EUROTEXT LTD, 2006.
  5. Romao, M.J.; Terao, M.. "Aldehyde Oxidoreductase (MOP)". In Handbook on Metalloproteins, 1037-1047. Wiley-VCH, 2001.
    Published • https://doi.org/10.1002/aoc.298
  6. Romão, Maria. "Dissimilatory Nitrate Reductase (NAP)". In Handbook on Metalloproteins, 1075-1085. CRC Press, 2001.
  7. Romão, Maria; Maria João Romão; Margarida Archer. "Cytochrome c´". In Handbook on Metalloproteins, edited by Albrecht Messerschmidt; Robert Huber; Thomas Poulas; Karl Wieghardt; Mirek Cygler; Wolfram Bode, 44-54. CRC Press, 2001.
    Published • 10.1002/0470028637.met103
Journal article
  1. Ana Rita Oliveira; Cristiano Mota; Guilherme Vilela-Alves; Rita Rebelo Manuel; Neide Pedrosa; Vincent Fourmond; Kateryna Klymanska; et al. "An allosteric redox switch involved in oxygen protection in a CO2 reductase". Nature Chemical Biology (2024): https://doi.org/10.1038/s41589-023-01484-2.
    10.1038/s41589-023-01484-2
  2. Filipa Engrola; Márcia A.S. Correia; Cameron Watson; Carlos C. Romão; Luis F. Veiros; Maria João Romão; Teresa Santos-Silva; Joanne M. Santini. "Arsenite oxidase in complex with antimonite and arsenite oxyanions: Insights into the catalytic mechanism". Journal of Biological Chemistry (2023): https://doi.org/10.1016/j.jbc.2023.105036.
    10.1016/j.jbc.2023.105036
  3. Diana Gomes; Márcia A. S. Correia; Maria João Romão; Passarinha LA; A. Sousa. "Integrated approaches for the separation and purification of recombinant HPV16 E6 protein from Escherichia coli crude extracts". Separation and Purification Technology 315 (2023): https://novaresearch.unl.pt/en/publications/93c1cd88-8aab-43b5-82f2-267758f9cd91.
    10.1016/j.seppur.2023.123647
  4. Cláudia S. Nóbrega; Ana Luísa Carvalho; Maria João Romão; Sofia R. Pauleta. "Structural Characterization of Neisseria gonorrhoeae Bacterial Peroxidase—Insights into the Catalytic Cycle of Bacterial Peroxidases". International Journal of Molecular Sciences (2023): https://doi.org/10.3390/ijms24076246.
    10.3390/ijms24076246
  5. Maria Pequito Luís; Inês Serrano Pereira; Joana N. Bugalhão; Catarina N. Simões; Cristiano De Sousa Mota; Maria João Romão; Luís Jaime Mota. "The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing Vacuole". Infection and Immunity (2023): e0040522-e0040522. https://novaresearch.unl.pt/en/publications/091db313-a23b-4772-b5c0-fd4200f8cfad.
    10.1128/iai.00405-22
  6. Marlene Duarte; Victor D. Alves; Márcia A. S. Correia; Catarina Caseiro; Luís M. A. Ferreira; Maria João Romão; Ana Luísa Carvalho; et al. "Structure-function studies can improve binding affinity of cohesin-dockerin interactions for multi-protein assemblies". International Journal of Biological Macromolecules 224 (2023): 55-67. https://novaresearch.unl.pt/en/publications/602f601b-7b8e-49f9-959c-81142822b4e6.
    10.1016/j.ijbiomac.2022.10.102
  7. Guilherme Vilela-Alves; Rita Rebelo Manuel; Ana Rita Oliveira; Inês Cardoso Pereira; Maria João Romão; Cristiano Mota. "Tracking W-Formate Dehydrogenase Structural Changes During Catalysis and Enzyme Reoxidation". International Journal of Molecular Sciences (2022): https://doi.org/10.3390/ijms24010476.
    10.3390/ijms24010476
  8. Ana M. Gonçalves; Augusto Q. Pedro; Diana M. Oliveira; Adriana E. Oliveira; Marino F. A. Santos; Márcia A. S. Correia; João A. Queiroz; et al. "Thermofluor-Based Optimization Strategy for the Stabilization of Recombinant Human Soluble Catechol-O-Methyltransferase". International Journal of Molecular Sciences (2022): https://doi.org/10.3390/ijms232012298.
    10.3390/ijms232012298
  9. Ana Rita Oliveira; Cristiano De Sousa Mota; Kateryna Klymanska; Frederic Biaso; Maria João Romão; Bruno Guigliarelli; Inês A. C. Pereira. "Spectroscopic and Structural Characterization of Reduced Desulfovibrio vulgaris Hildenborough W-FdhAB Reveals Stable Metal Coordination during Catalysis". ACS Chemical Biology 17 7 (2022): 1901-1909. https://novaresearch.unl.pt/en/publications/ac7b6d91-05ca-4242-8559-7df12cef0ec0.
    10.1021/acschembio.2c00336
  10. Ana Margarida Grilo Gonçalves; Ângela Sousa; Augusto Q. Pedro; Maria João Romão; João A. Queiroz; Eugénia Gallardo; Passarinha LA. "Advances in Membrane-Bound Catechol-O-Methyltransferase Stability Achieved Using a New Ionic Liquid-Based Storage Formulation". International Journal of Molecular Sciences 23 13 (2022): https://novaresearch.unl.pt/en/publications/53640278-bf26-47d8-9cf3-9c5e14938d98.
    10.3390/ijms23137264
  11. Cristiano De Sousa Mota; Ana Marta Alves Diniz; Catarina Coelho; Teresa Santos-Silva; Mariam Esmaeeli; Silke Leimkühler; Eurico J Cabrita; Filipa Marcelo; Maria João Romão. "Interrogating the Inhibition Mechanisms of Human Aldehyde Oxidase by X-ray Crystallography and NMR Spectroscopy: The Raloxifene Case". Journal Of Medicinal Chemistry 64 17 (2021): 13025-13037. https://novaresearch.unl.pt/en/publications/bd99440e-43d4-440c-be37-a84292f0ba18.
    10.1021/acs.jmedchem.1c01125
  12. Mariella Polino; Hoon-suk Rho; M. P. Pina; Reys Mallada; Ana Luísa Carvalho; Maria João Romão; Isabel Coelhoso; et al. "Protein crystallization in a microfluidic contactor with nafion®117 membranes". Membranes 11 8 (2021): https://novaresearch.unl.pt/en/publications/b679470a-d208-4d9e-bf9c-cd5de2ed3f0d.
    10.3390/membranes11080549
  13. Raquel dos Santos; Maria João Romão; Roque, A.C.A.; Ana Luísa Carvalho. "Magnetic particles used in a new approach for designed protein crystallization". CrystEngComm 23 5 (2021): 1083-1090. https://novaresearch.unl.pt/en/publications/8986cc88-9b6d-4de2-9427-6e161e3790eb.
    10.1039/d0ce01529f
  14. Rita Lopes; Álvaro Raya-Barón; M. Paula Robalo; Carolina Vinagreiro; Barroso, S. B.; Maria João Romão; Ignacio Fernández; Mariette M. Pereira; Beatriz Royo. "Donor Functionalized Iron(II) N-Heterocyclic Carbene Complexes in Transfer Hydrogenation Reactions". European Journal of Inorganic Chemistry 2021 1 (2021): 22-29. https://novaresearch.unl.pt/en/publications/900706f4-c01a-48d2-bdda-0cddf2a442ac.
    10.1002/ejic.202000868
  15. Francisco Leisico; Lia M. Godinho; Inês C. Gonçalves; Sara P. Silva; Bruno Carneiro; Maria João Romão; Teresa Santos-Silva; et al. "Multitask ATPases (NBDs) of bacterial ABC importers type I and their interspecies exchangeability". Scientific Reports 10 1 (2020): https://novaresearch.unl.pt/en/publications/b85b8cce-c518-4679-9df5-43bc2d76931f.
    Published • 10.1038/s41598-020-76444-0
  16. Pedro Ferreira; Nuno M. F. Sousa A. Cerqueira; Pedro Alexandrino Fernandes; Maria João Romão; Maria João Ramos. "Catalytic Mechanism of Human Aldehyde Oxidase". ACS Catalysis 10 16 (2020): 9276-9286. https://novaresearch.unl.pt/en/publications/6743d222-2ae8-464c-84e0-fa6e282315d5.
    10.1021/acscatal.0c02627
  17. Ana Sara Gomes; Helena Ramos; Sara Gomes; Joana B. Loureiro; Joana Soares; Valentina Barcherini; Paola Monti; et al. "SLMP53-1 interacts with wild-type and mutant p53 DNA-binding domain and reactivates multiple hotspot mutations". Biochimica et Biophysica Acta - General Subjects 1864 1 (2020): https://novaresearch.unl.pt/en/publications/835b29c1-bec5-44df-8a20-8167fd34ec7c.
    10.1016/j.bbagen.2019.129440
  18. Fernandes, A.R.; Mendonça-Martins, I.; Santos, M.F.A.; Raposo, L.R.; Mendes, R.; Marques, J.; Romão, C.C.; et al. "Improving the Anti-inflammatory Response via Gold Nanoparticle Vectorization of CO-Releasing Molecules". ACS Biomaterials Science and Engineering 6 2 (2020): 1090-1101. http://www.scopus.com/inward/record.url?eid=2-s2.0-85079071744&partnerID=MN8TOARS.
    10.1021/acsbiomaterials.9b01936
  19. Oliveira, A.R.; Mota, C.; Mourato, C.; Domingos, R.M.; Santos, M.F.A.; Gesto, D.; Guigliarelli, B.; et al. "Toward the Mechanistic Understanding of Enzymatic CO2 Reduction". ACS Catalysis 10 6 (2020): 3844-3856. http://www.scopus.com/inward/record.url?eid=2-s2.0-85082073064&partnerID=MN8TOARS.
    10.1021/acscatal.0c00086
  20. Catarina Coelho; Jayaraman Muthukumaran; Teresa Santos-Silva; Maria João Romão. "Systematic exploration of predicted destabilizing nonsynonymous single nucleotide polymorphisms (nsSNPs) of human aldehyde oxidase: A Bio-informatics study". Pharmacology Research & Perspectives 7 6 (2019): https://doi.org/10.1002/prp2.538.
    10.1002/prp2.538
  21. Cristiano De Sousa Mota; Mariam Esmaeeli; Catarina Coelho; Teresa Santos-Silva; Martin Wolff; Alessandro Foti; Silke Leimkühler; Maria João Romão. "Human aldehyde oxidase (hAOX1): structure determination of the Moco-free form of the natural variant G1269R and biophysical studies of single nucleotide polymorphisms". FEBS Open Bio 9 5 (2019): 925-934. https://novaresearch.unl.pt/en/publications/4ed52cd7-52c6-4de6-b539-1fecaa355682.
    10.1002/2211-5463.12617
  22. Martins, F.F.; Joseph, A.; Diogo, H.P.; Minas da Piedade, M.E.; Ferreira, L.P.; Carvalho, M.D.; Barroso, S.; et al. "Irreversible Magnetic Behaviour Caused by the Thermosalient Phenomenon in an Iron(III) Spin Crossover Complex". European Journal of Inorganic Chemistry 2018 25 (2018): 2976-2983. http://www.scopus.com/inward/record.url?eid=2-s2.0-85049604005&partnerID=MN8TOARS.
    10.1002/ejic.201800605
  23. Mota, C.; Coelho, C.; Leimkühler, S.; Garattini, E.; Terao, M.; Santos-Silva, T.; Romão, M.J.. "Critical overview on the structure and metabolism of human aldehyde oxidase and its role in pharmacokinetics". Coordination Chemistry Reviews 368 (2018): 35-59. http://www.scopus.com/inward/record.url?eid=2-s2.0-85046125951&partnerID=MN8TOARS.
    10.1016/j.ccr.2018.04.006
  24. Leisico, F.; Vieira, D.V.; Figueiredo, T.A.; Silva, M.; Cabrita, E.J.; Sobral, R.G.; Ludovice, A.M.; et al. "First insights of peptidoglycan amidation in Gram-positive bacteria-The high-resolution crystal structure of Staphylococcus aureus glutamine amidotransferase GatD". Scientific Reports 8 1 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85044530342&partnerID=MN8TOARS.
    10.1038/s41598-018-22986-3
  25. Kowacz, Magdalena; Marchel, Mateusz; Juknaite, Lina; Esperanca, Jose M. S. S.; Romao, Maria Joao; Carvalho, Ana Luisa; Rebelo, Luis Paulo N.. "Infrared light-induced protein crystallization. Structuring of protein interfacial water and periodic self-assembly". Journal of Crystal Growth 457 (2017): 362-368. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000389590700063&KeyUID=WOS:000389590700063.
    10.1016/j.jcrysgro.2016.01.003
  26. Watson, C.; Niks, D.; Hille, R.; Vieira, M.; Schoepp-Cothenet, B.; Marques, A.T.; Romão, M.J.; Santos-Silva, T.; Santini, J.M.. "Electron transfer through arsenite oxidase: Insights into Rieske interaction with cytochrome c". Biochimica et Biophysica Acta - Bioenergetics 1858 10 (2017): 865-872. http://www.scopus.com/inward/record.url?eid=2-s2.0-85027491816&partnerID=MN8TOARS.
    10.1016/j.bbabio.2017.08.003
  27. Pires, V.M.R.; Pereira, P.M.M.; Brás, J.L.A.; Correia, M.; Cardoso, V.; Bule, P.; Alves, V.D.; et al. "Stability and ligand promiscuity of type A carbohydrate-binding modules are illustrated by the structure of Spirochaeta thermophila StCBM64C". Journal of Biological Chemistry 292 12 (2017): 4847-4860. http://www.scopus.com/inward/record.url?eid=2-s2.0-85016255875&partnerID=MN8TOARS.
    10.1074/jbc.M116.767541
  28. Romão, M.J.; Coelho, C.; Santos-Silva, T.; Foti, A.; Terao, M.; Garattini, E.; Leimkühler, S.. "Structural basis for the role of mammalian aldehyde oxidases in the metabolism of drugs and xenobiotics". Current Opinion in Chemical Biology 37 (2017): 39-47. http://www.scopus.com/inward/record.url?eid=2-s2.0-85010465431&partnerID=MN8TOARS.
    10.1016/j.cbpa.2017.01.005
  29. Serrano, J.L.; Cavalheiro, E.; Barroso, S.; Romão, M.J.; Silvestre, S.; Almeida, P.. "A synthetic route to novel 3-substituted-2,1-benzisoxazoles from 5-(2-nitrobenzylidene)(thio)barbiturates". Comptes Rendus Chimie 20 11-12 (2017): 990-995. http://www.scopus.com/inward/record.url?eid=2-s2.0-85034619355&partnerID=MN8TOARS.
    10.1016/j.crci.2017.10.002
  30. Polino, M.; Carvalho, A.L.; Juknaite, L.; Portugal, C.A.M.; Coelhoso, I.M.; Romão, M.J.; Crespo, J.G.. "Ion-Exchange Membranes for Stable Derivatization of Protein Crystals". Crystal Growth and Design 17 9 (2017): 4563-4572. http://www.scopus.com/inward/record.url?eid=2-s2.0-85028995862&partnerID=MN8TOARS.
    10.1021/acs.cgd.7b00315
  31. Foti, Alessandro; Hartmann, Tobias; Coelho, Catarina; Santos-Silva, Teresa; Romao, Maria Joao; Leimkuehler, Silke. "Optimization of the Expression of Human Aldehyde Oxidase for Investigations of Single-Nucleotide Polymorphisms". Drug Metabolism and Disposition 44 8 (2016): 1277-1285. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000379415700017&KeyUID=WOS:000379415700017.
    10.1124/dmd.115.068395
  32. Bras, Joana L. A.; Pinheiro, Benedita A.; Cameron, Kate; Cuskin, Fiona; Viegas, Aldino; Najmudin, Shabir; Bule, Pedro; et al. "Diverse specificity of cellulosome attachment to the bacterial cell surface". Scientific Reports 6 (2016): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000389700700001&KeyUID=WOS:000389700700001.
    10.1038/srep38292
  33. Correia, Marcia A. S.; Otrelo-Cardoso, Ana Rita; Schwuchow, Viola; Clauss, Kajsa G. V. Sigfridsson; Haumann, Michael; Romao, Maria Joao; Leimkuhler, Silke; Santos-Silva, Teresa. "The Escherichia coli Periplasmic Aldehyde Oxidoreductase Is an Exceptional Member of the Xanthine Oxidase Family of Molybdoenzymes". Acs Chemical Biology 11 10 (2016): 2923-2935. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000386187700030&KeyUID=WOS:000386187700030.
    10.1021/acschembio.6b00572
  34. Terao, Mineko; Romao, Maria Joao; Leimkuehler, Silke; Bolis, Marco; Fratelli, Maddalena; Coelho, Catarina; Santos-Silva, Teresa; Garattini, Enrico. "Structure and function of mammalian aldehyde oxidases". Archives of Toxicology 90 4 (2016): 753-780. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000372427900001&KeyUID=WOS:000372427900001.
    10.1007/s00204-016-1683-1
  35. Magdalena Kowacz; M. Marchel; Lina Juknaité; J. M. S. S. Esperança; Maria João Romão; Ana Luísa Carvalho; Luis Paulo Rebelo. "Ionic-Liquid-Functionalized Mineral Particles for Protein Crystallization". Crystal Growth & Design 15 6 (2015): 2994-3003. https://novaresearch.unl.pt/en/publications/c3d5624c-089b-412a-9563-8931ee5b813b.
    10.1021/acs.cgd.5b00403
  36. Coelho, Catarina; Romao, Maria Joao. "Structural and mechanistic insights on nitrate reductases". Protein Science 24 12 (2015): 1901-1911. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000368292000001&KeyUID=WOS:000368292000001.
    10.1002/pro.2801
  37. Seixas, Joao D.; Santos, Marino F. A.; Mukhopadhyay, Abhik; Coelho, Ana C.; Reis, Patricia M.; Veiros, Luis F.; Marques, Ana R.; et al. "A contribution to the rational design of Ru(CO)(3)Cl2L complexes for in vivo delivery of CO". Dalton Transactions 44 11 (2015): 5058-5075. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000350488300027&KeyUID=WOS:000350488300027.
    10.1039/c4dt02966f
  38. Correia, H.D.; Marangon, J.; Brondino, C.D.; Moura, J.J.G.; Romão, M.J.; González, P.J.; Santos-Silva, T.. "Erratum: Aromatic aldehydes at the active site of aldehyde oxidoreductase from Desulfovibrio gigas: Reactivity and molecular details of the enzyme-substrate and enzyme-product interaction(Journal of Biological Inorganic Chemistry(2015) 20:231)". Journal of Biological Inorganic Chemistry 20 2 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84925463018&partnerID=MN8TOARS.
    10.1007/s00775-014-1205-7
  39. Correia, H.D.; Marangon, J.; Brondino, C.D.; Moura, J.J.G.; Romão, M.J.; González, P.J.; Santos-Silva, T.. "Aromatic aldehydes at the active site of aldehyde oxidoreductase from Desulfovibrio gigas: Reactivity and molecular details of the enzyme-substrate and enzyme-product interaction". Journal of Biological Inorganic Chemistry 20 2 (2015): 219-229. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925539562&partnerID=MN8TOARS.
    10.1007/s00775-014-1196-4
  40. Cerqueira, N.M.F.S.A.; Coelho, C.; Brás, N.F.; Fernandes, P.A.; Garattini, E.; Terao, M.; Romão, M.J.; Ramos, M.J.. "Insights into the structural determinants of substrate specificity and activity in mouse aldehyde oxidases". Journal of Biological Inorganic Chemistry 20 2 (2015): 209-217. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925496582&partnerID=MN8TOARS.
    10.1007/s00775-014-1198-2
  41. Coelho, Catarina; Foti, Alessandro; Hartmann, Tobias; Santos-Silva, Teresa; Leimkuehler, Silke; Romao, Maria Joao; Coelho, C.; et al. "Structural insights into xenobiotic and inhibitor binding to human aldehyde oxidase". Nature Chemical Biology 11 10 (2015): 779-+. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000361536500010&KeyUID=WOS:000361536500010.
    10.1038/NCHEMBIO.1895
  42. Otrelo-Cardoso, Ana Rita; da Silva Correia, Marcia Alexandra; Schwuchow, Viola; Svergun, Dmitri I.; Romao, Maria Joao; Leimkuehler, Silke; Santos-Silva, Teresa. "Structural Data on the Periplasmic Aldehyde Oxidoreductase PaoABC from Escherichia coli: SAXS and Preliminary X-ray Crystallography Analysis". International Journal of Molecular Sciences 15 2 (2014): 2223-2236. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000335776400032&KeyUID=WOS:000335776400032.
    10.3390/ijms15022223
  43. Ribeiro, Diana; Kulakova, Alina; Quaresma, Pedro; Pereira, Eulalia; Bonifacio, Cecilia; Romao, Maria Joao; Franco, Ricardo; Carvalho, Ana Luisa. "Use of Gold Nanoparticles as Additives in Protein Crystallization". Crystal Growth & Design 14 1 (2014): 222-227. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000329337000029&KeyUID=WOS:000329337000029.
    10.1021/cg4014398
  44. Otrelo-Cardoso, Ana Rita; Schwuchow, Viola; Rodrigues, David; Cabrita, Eurico J.; Leimkuehler, Silke; Romao, Maria Joao; Santos-Silva, Teresa. "Biochemical, Stabilization and Crystallization Studies on a Molecular Chaperone (PaoD) Involved in the Maturation of Molybdoenzymes". Plos One 9 1 (2014): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000330621900083&KeyUID=WOS:000330621900083.
    10.1371/journal.pone.0087295
  45. Ribeiro, D.; Kulakova, A.; Quaresma, P.; Pereira, E.; Bonifácio, C.; Romão, M.J.; Franco, R.; Carvalho, A.L.. "Erratum: Use of gold nanoparticles as additives in protein crystallization (Crystal Growth and Design (2014) 14:1 (222-227) DOI:10.1021/cg4014398)". Crystal Growth and Design 14 2 (2014): 888-888. http://www.scopus.com/inward/record.url?eid=2-s2.0-84893646478&partnerID=MN8TOARS.
    10.1021/cg4018745
  46. Viegas, A; João Sardinha; Filipe Freire; Daniel F. Duarte; Ana Luísa Carvalho; Carlos M. G. A. Fontes; Maria João Romão; et al. "Solution structure, dynamics and binding studies of a family 11 carbohydrate-binding module from Clostridium thermocellum ( Ct CBM11)". Biochemical Journal 451 2 (2013): 289-300. https://novaresearch.unl.pt/en/publications/2143fff1-8d2f-407d-b7e3-ba6d9e899a56.
    10.1042/bj20120627
  47. Mahro, Martin; Bras, Natercia F.; Cerqueira, Nuno M. F. S. A.; Teutloff, Christian; Coelho, Catarina; Romao, Maria Joao; Leimkuehler, Silke. "Identification of Crucial Amino Acids in Mouse Aldehyde Oxidase 3 That Determine Substrate Specificity". Plos One 8 12 (2013): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000328735700053&KeyUID=WOS:000328735700053.
    10.1371/journal.pone.0082285
  48. Verma, Anil Kumar; Goyal, Arun; Freire, Filipe; Bule, Pedro; Venditto, Immacolata; Bras, Joana L. A.; Santos, Helena; et al. "Overexpression, crystallization and preliminary X-ray crystallographic analysis of glucuronoxylan xylanohydrolase (Xyn30A) from Clostridium thermocellum". Acta Crystallographica Section F-Structural Biology and Crystallization Communications 69 (2013): 1440-1442. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000327942200030&KeyUID=WOS:000327942200030.
    10.1107/S1744309113025050
  49. Marangon, Jacopo; Correia, Hugo D.; Brondino, Carlos D.; Moura, Jose J. G.; Romao, Maria J.; Gonzalez, Pablo J.; Santos-Silva, Teresa; et al. "Kinetic and Structural Studies of Aldehyde Oxidoreductase from Desulfovibrio gigas Reveal a Dithiolene-Based Chemistry for Enzyme Activation and Inhibition by H2O2". Plos One 8 12 (2013): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000329325200045&KeyUID=WOS:000329325200045.
    10.1371/journal.pone.0083234
  50. Seixas, J.D.; Mukhopadhyay, A.; Santos-Silva, T.; Otterbein, L.E.; Gallo, D.J.; Rodrigues, S.S.; Guerreiro, B.H.; et al. "Characterization of a versatile organometallic pro-drug (CORM) for experimental CO based therapeutics". Dalton Transactions 42 17 (2013): 5985-5998. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875795564&partnerID=MN8TOARS.
    10.1039/c2dt32174b
  51. Mehtab, S.; Gonçalves, G.; Roy, S.; Tomaz, A.I.; Santos-Silva, T.; Santos, M.F.A.; Romão, M.J.; et al. "Interaction of vanadium(IV) with human serum apo-transferrin". Journal of Inorganic Biochemistry 121 (2013): 187-195. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873700367&partnerID=MN8TOARS.
    10.1016/j.jinorgbio.2012.12.020
  52. Coelho, C.; Marangon, J.; Rodrigues, D.; Moura, J.J.G.; Romão, M.J.; Paes De Sousa, P.M.; Correia Dos Santos, M.M.. "Induced peroxidase activity of haem containing nitrate reductases revealed by protein film electrochemistry". Journal of Electroanalytical Chemistry 693 (2013): 105-113. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874694114&partnerID=MN8TOARS.
    10.1016/j.jelechem.2013.01.030
  53. Aveiro, S. S.; Freire, F.; Clayton, J.; Cameloc, M.; Carvalho, A. L.; Ferreira, G. C.; Romao, M. J.; et al. "Structural studies of the p22HBP/SOUL family of heme-binding proteins". Febs Journal 279 (2012): 458-458. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000308128602495&KeyUID=WOS:000308128602495.
  54. Brás, J.L.A.; Alves, V.D.; Carvalho, A.L.; Najmudin, S.; Prates, J.A.M.; Ferreira, L.M.A.; Bolam, D.N.; et al. "Novel Clostridium thermocellum type I cohesin-dockerin complexes reveal a single binding mode". Journal of Biological Chemistry 287 53 (2012): 44394-44405. http://www.scopus.com/inward/record.url?eid=2-s2.0-84871786607&partnerID=MN8TOARS.
    10.1074/jbc.M112.407700
  55. Santos, M.F.A.; Seixas, J.D.; Coelho, A.C.; Mukhopadhyay, A.; Reis, P.M.; Romão, M.J.; Romão, C.C.; Santos-Silva, T.. "New insights into the chemistry of fac-[Ru(CO)3]2 + fragments in biologically relevant conditions: The CO releasing activity of [Ru(CO)3Cl2(1,3-thiazole)], and the X-ray crystal structure of its adduct with lysozyme". Journal of Inorganic Biochemistry 117 (2012): 285-291. http://www.scopus.com/inward/record.url?eid=2-s2.0-84869090746&partnerID=MN8TOARS.
    10.1016/j.jinorgbio.2012.06.018
  56. Coelho, C.; Mahro, M.; Trincão, J.; Carvalho, A.T.P.; Ramos, M.J.; Terao, M.; Garattini, E.; Leimkühler, S.; Romão, M.J.. "The first mammalian aldehyde oxidase crystal structure: Insights into substrate specificity". Journal of Biological Chemistry 287 48 (2012): 40690-40702. http://www.scopus.com/inward/record.url?eid=2-s2.0-84870012251&partnerID=MN8TOARS.
    10.1074/jbc.M112.390419
  57. Kowacz, M.; Mukhopadhyay, A.; Carvalho, A.L.; Esperança, J.M.S.S.; Romão, M.J.; Rebelo, L.P.N.. "Hofmeister effects of ionic liquids in protein crystallization: Direct and water-mediated interactions". CrystEngComm 14 15 (2012): 4912-4921. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863707784&partnerID=MN8TOARS.
    10.1039/c2ce25129a
  58. Fontes, C.M.G.A.; Correia, I.S.; Romão, M.J.; Sá-Nogueira, I.; Prates, J.A.M.; Ferreira, L.M.A.. "Editorial". Biocatalysis and Biotransformation 30 3 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84861752070&partnerID=MN8TOARS.
    10.3109/10242422.2012.681858
  59. Luís, A.S.; Alves, V.D.; Romão, M.J.; Prates, J.A.M.; Fontes, C.M.G.A.; Najmudin, S.. "Overproduction, purification, crystallization and preliminary X-ray characterization of a novel carbohydrate-binding module of endoglucanase Cel5A from Eubacterium cellulosolvens". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 67 4 (2011): 491-493. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955111233&partnerID=MN8TOARS.
    10.1107/S1744309111004246
  60. Santos-Silva, T.; Mukhopadhyay, A.; Seixas, J.D.; Bernardes, G.J.L.; Romão, C.C.; Romão, M.J.. "CORM-3 reactivity toward proteins: The crystal structure of a Ru(II) dicarbonyl-lysozyme complex". Journal of the American Chemical Society 133 5 (2011): 1192-1195. http://www.scopus.com/inward/record.url?eid=2-s2.0-79551692596&partnerID=MN8TOARS.
    10.1021/ja108820s
  61. Mukhopadhyay, A.; Kladova, A.V.; Bursakov, S.A.; Gavel, O.Yu.; Calvete, J.J.; Shnyrov, V.L.; Moura, I.; et al. "Crystal structure of the zinc-, cobalt-, and iron-containing adenylate kinase from Desulfovibrio gigas: A novel metal-containing adenylate kinase from Gram-negative bacteria". Journal of Biological Inorganic Chemistry 16 1 (2011): 51-61. http://www.scopus.com/inward/record.url?eid=2-s2.0-79951555584&partnerID=MN8TOARS.
    10.1007/s00775-010-0700-8
  62. Brás, J.L.A.; Correia, M.A.S.; Romão, M.J.; Prates, J.A.M.; Fontes, C.M.G.A.; Najmudin, S.. "Purification, crystallization and preliminary X-ray characterization of the pentamodular arabinoxylanase CtXyl5A from Clostridium thermocellum". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 67 7 (2011): 833-836. http://www.scopus.com/inward/record.url?eid=2-s2.0-79960949534&partnerID=MN8TOARS.
    10.1107/S1744309111020823
  63. Coelho, C.; González, P.J.; Moura, J.G.; Moura, I.; Trincão, J.; João Romão, M.. "The crystal structure of cupriavidus necator nitrate reductase in oxidized and partially reduced states". Journal of Molecular Biology 408 5 (2011): 932-948. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955478107&partnerID=MN8TOARS.
    10.1016/j.jmb.2011.03.016
  64. García-Alvarez, B.; Melero, R.; Dias, F.M.V.; Prates, J.A.M.; Fontes, C.M.G.A.; Smith, S.P.; Romão, M.J.; Carvalho, A.L.; Llorca, O.. "Molecular architecture and structural transitions of a clostridium thermocellum mini-cellulosome". Journal of Molecular Biology 407 4 (2011): 571-580. http://www.scopus.com/inward/record.url?eid=2-s2.0-79952449019&partnerID=MN8TOARS.
    10.1016/j.jmb.2011.01.060
  65. Mahro, M.; Coelho, C.; Trincão, J.; Rodrigues, D.; Terao, M.; Garattini, E.; Saggu, M.; et al. "Characterization and crystallization of mouse aldehyde oxidase 3: From mouse liver to Escherichia coli heterologous protein expression". Drug Metabolism and Disposition 39 10 (2011): 1939-1945. http://www.scopus.com/inward/record.url?eid=2-s2.0-80053139530&partnerID=MN8TOARS.
    10.1124/dmd.111.040873
  66. Santos-Silva, T.; Mukhopadhyay, A.; Seixas, J.D.; Bernardes, G.J.L.; Romão, C.C.; Romão, M.J.. "Towards improved therapeutic CORMs: Understanding the reactivity of CORM-3 with proteins". Current Medicinal Chemistry 18 22 (2011): 3361-3366. http://www.scopus.com/inward/record.url?eid=2-s2.0-79961084009&partnerID=MN8TOARS.
    10.2174/092986711796504583
  67. Brás, J.L.A.; Cartmell, A.; Carvalho, A.L.M.; Verzé, G.; Bayer, E.A.; Vazana, Y.; Correia, M.A.S.; et al. "Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis". Proceedings of the National Academy of Sciences of the United States of America 108 13 (2011): 5237-5242. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955116598&partnerID=MN8TOARS.
    10.1073/pnas.1015006108
  68. Najmudin, S.; Pinheiro, B.A.; Prates, J.A.M.; Gilbert, H.J.; Romão, M.J.; Fontes, C.M.G.A.. "Putting an N-terminal end to the Clostridium thermocellum xylanase Xyn10B story: Crystal structure of the CBM22-1-GH10 modules complexed with xylohexaose". Journal of Structural Biology 172 3 (2010): 353-362. http://www.scopus.com/inward/record.url?eid=2-s2.0-77957964845&partnerID=MN8TOARS.
    10.1016/j.jsb.2010.07.009
  69. Ribeiro, T.; Santos-Silva, T.; Alves, V.D.; Dias, F.M.V.; Luís, A.S.; Prates, J.A.M.; Ferreira, L.M.A.; Romão, M.J.; Fontes, C.M.G.A.. "Family 42 carbohydrate-binding modules display multiple arabinoxylan-binding interfaces presenting different ligand affinities". Biochimica et Biophysica Acta - Proteins and Proteomics 1804 10 (2010): 2054-2062. http://www.scopus.com/inward/record.url?eid=2-s2.0-77956061008&partnerID=MN8TOARS.
    10.1016/j.bbapap.2010.07.006
  70. Najmudin, S.; Pauleta, S.R.; Moura, I.; Romão, M.J.. "The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 66 6 (2010): 627-635. http://www.scopus.com/inward/record.url?eid=2-s2.0-77953142481&partnerID=MN8TOARS.
    10.1107/S1744309110013989
  71. Coelho, C.; Trincão, J.; João Romão, M.. "The use of ionic liquids as crystallization additives allowed to overcome nanodrop scaling up problems: A success case for producing diffraction-quality crystals of a nitrate reductase". Journal of Crystal Growth 312 5 (2010): 714-719. http://www.scopus.com/inward/record.url?eid=2-s2.0-74949128363&partnerID=MN8TOARS.
    10.1016/j.jcrysgro.2009.11.060
  72. Cerqueira, N.M.F.S.A.; Gonzalez, P.J.; Brondino, C.D.; Romão, M.J.; Romão, C.C.; Moura, I.; Moura, J.J.G.. "The effect of the sixth sulfur ligand in the catalytic mechanism of periplasmic nitrate reductase". Journal of Computational Chemistry 30 15 (2009): 2466-2484. http://www.scopus.com/inward/record.url?eid=2-s2.0-70349913999&partnerID=MN8TOARS.
    10.1002/jcc.21280
  73. Viciosa, M.T.; Correia, N.T.; Salmerón Sanchez, M.; Carvalho, A.L.; Romão, M.J.; Gómez Ribelles, J.L.; Dionísio, M.. "Real-time monitoring of molecular dynamics of ethylene glycol dimethacrylate glass former". Journal of Physical Chemistry B 113 43 (2009): 14209-14217. http://www.scopus.com/inward/record.url?eid=2-s2.0-70350375870&partnerID=MN8TOARS.
    10.1021/jp903212g
  74. Santos-Silva, T.; Ferroni, F.; Thapper, A.; Marangon, J.; González, P.J.; Rizzi, A.C.; Moura, I.; et al. "Kinetic, structural, and EPR studies reveal that aldehyde oxidoreductase from Desulfovibrio gigas does not need a sulfido ligand for catalysis and give evidence for a direct Mo-C interaction in a biological system". Journal of the American Chemical Society 131 23 (2009): 7990-7998. http://www.scopus.com/inward/record.url?eid=2-s2.0-67650531126&partnerID=MN8TOARS.
    10.1021/ja809448r
  75. Freire, F.; Romão, M.J.; MacEdo, A.L.; Aveiro, S.S.; Goodfellow, B.J.; Carvalho, A.L.. "Preliminary structural characterization of human SOUL, a haem-binding protein". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 65 7 (2009): 723-726. http://www.scopus.com/inward/record.url?eid=2-s2.0-68149089746&partnerID=MN8TOARS.
    10.1107/S174430910902291X
  76. Honzícek, J.; Mukhopadhyay, A.; Santos-Silva, T.; Romão, M.J.; Romão, C.C.. "Ring-functionalized molybdenocene complexes". Organometallics 28 9 (2009): 2871-2879. http://www.scopus.com/inward/record.url?eid=2-s2.0-66149128384&partnerID=MN8TOARS.
    10.1021/om9001022
  77. Romão, M.J.. "Molybdenum and tungsten enzymes: a crystallographic and mechanistic overview". Dalton Transactions 21 (2009): 4053-4068. http://www.scopus.com/inward/record.url?eid=2-s2.0-65949088825&partnerID=MN8TOARS.
    10.1039/b821108f
  78. Najmudin, Shabir; Bonifacio, Cecilia; Duarte, Americo G.; Pauleta, Sofia R.; Moura, Isabel; Moura, Jose J. G.; Romao, Maria J.; et al. "Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas. (vol F65, pg 730, 2009)". Acta Crystallographica Section F-Structural Biology and Crystallization Communications 65 NA (2009): 856-856. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000268517300030&KeyUID=WOS:000268517300030.
    10.1107/S174430910902644X
  79. Kladova, A.V.; Gavel, O.Y.; Mukhopaadhyay, A.; Boer, D.R.; Teixeira, S.; Shnyrov, V.L.; Moura, I.; et al. "Cobalt-, zinc- and iron-bound forms of adenylate kinase (AK) from the sulfate-reducing bacterium Desulfovibrio gigas: Purification, crystallization and preliminary X-ray diffraction analysis". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 65 9 (2009): 926-929. http://www.scopus.com/inward/record.url?eid=2-s2.0-69849114877&partnerID=MN8TOARS.
    10.1107/S1744309109029157
  80. Carvalho, A.L.; Trincão, J.; Romão, M.J.; Carvalho, Ana Luísa; Trincão, José; Romão, Maria João; Carvalho, Ana Luisa; et al. "X-ray crystallography in drug discovery.". Methods in molecular biology (Clifton, N.J.) 572 (2009): 31-56. http://www.scopus.com/inward/record.url?eid=2-s2.0-79952112755&partnerID=MN8TOARS.
    10.1007/978-1-60761-244-5_3
  81. Najmudin, S.; Pinheiro, B.A.; Romão, M.J.; Prates, J.A.M.; Fontes, C.M.G.A.. "Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-ß-D-xylanase 10B in complex with xylohexaose". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 64 8 (2008): 715-718. http://www.scopus.com/inward/record.url?eid=2-s2.0-49249121067&partnerID=MN8TOARS.
    10.1107/S1744309108019696
  82. Vidinha, P.; Lourenço, N.M.T.; Pinheiro, C.; Brás, A.R.; Carvalho, T.; Santos-Silva, T.; Mukhopadhyay, A.; et al. "Ion jelly: A tailor-made conducting material for smart electrochemical devices". Chemical Communications 44 (2008): 5842-5844. http://www.scopus.com/inward/record.url?eid=2-s2.0-56349138178&partnerID=MN8TOARS.
    10.1039/b811647d
  83. Najmudin, S.; González, P.J.; Trincão, J.; Coelho, C.; Mukhopadhyay, A.; Cerqueira, N.M.F.S.A.; Romão, C.C.; et al. "Periplasmic nitrate reductase revisited: A sulfur atom completes the sixth coordination of the catalytic molybdenum". Journal of Biological Inorganic Chemistry 13 5 (2008): 737-753. http://www.scopus.com/inward/record.url?eid=2-s2.0-44549083087&partnerID=MN8TOARS.
    10.1007/s00775-008-0359-6
  84. Chaves, S.; Gil, M.; Canário, S.; Jelic, R.; Romão, M.J.; Trincão, J.; Herdtweck, E.; et al. "Biologically relevant O,S-donor compounds. Synthesis, molybdenum complexation and xanthine oxidase inhibition". Dalton Transactions n/d 13 (2008): 1773-1782. http://www.scopus.com/inward/record.url?eid=2-s2.0-40849130962&partnerID=MN8TOARS.
    10.1039/b717172b
  85. Viegas, A.; Brás, N.F.; Cerqueira, N.M.F.S.A.; Fernandes, P.A.; Prates, J.A.M.; Fontes, C.M.G.A.; Bruix, M.; et al. "Molecular determinants of ligand specificity in family 11 carbohydrate binding modules - An NMR, X-ray crystallography and computational chemistry approach". FEBS Journal 275 10 (2008): 2524-2535. http://www.scopus.com/inward/record.url?eid=2-s2.0-42649116746&partnerID=MN8TOARS.
    10.1111/j.1742-4658.2008.06401.x
  86. Coelho, C.; González, P.J.; Trincão, J.; Carvalho, A.L.; Najmudin, S.; Hettman, T.; Dieckman, S.; et al. "Heterodimeric nitrate reductase (NapAB) from Cupriavidus necator H16: Purification, crystallization and preliminary X-ray analysis". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 63 6 (2007): 516-519. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249945097&partnerID=MN8TOARS.
    10.1107/S1744309107022129
  87. Santos-Silva, T.; Dias, J.M.; Dolla, A.; Durand, M.-C.; Gonçalves, L.L.; Lampreia, J.; Moura, I.; Romão, M.J.. "Crystal Structure of the 16 Heme Cytochrome from Desulfovibrio gigas: A Glycosylated Protein in a Sulphate-reducing Bacterium". Journal of Molecular Biology 370 4 (2007): 659-673. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249950618&partnerID=MN8TOARS.
    10.1016/j.jmb.2007.04.055
  88. Brondino, C.D.; Rivas, M.G.; Romão, M.J.; Moura, J.J.G.; Moura, I.. "Erratum: Structural and electron paramagnetic resonance (EPR) studies of mononuclear molybdenum enzymes from sulfate-reducing bacteria (Accounts of Chemical Research (2006) 39, (793))". Accounts of Chemical Research 40 3 (2007): http://www.scopus.com/inward/record.url?eid=2-s2.0-34147199869&partnerID=MN8TOARS.
    10.1021/ar600049k
  89. Thapper, A.; Boer, D.R.; Brondino, C.D.; Moura, J.J.G.; Romão, M.J.. "Correlating EPR and X-ray structural analysis of arsenite-inhibited forms of aldehyde oxidoreductase". Journal of Biological Inorganic Chemistry 12 3 (2007): 353-366. http://www.scopus.com/inward/record.url?eid=2-s2.0-34147108128&partnerID=MN8TOARS.
    10.1007/s00775-006-0191-9
  90. Carvalho, A.L.; Dias, F.M.V.; Nagy, T.; Prates, J.A.M.; Proctor, M.R.; Smith, N.; Bayer, E.A.; et al. "Evidence for a dual binding mode of dockerin modules to cohesins". Proceedings of the National Academy of Sciences of the United States of America 104 9 (2007): 3089-3094. http://www.scopus.com/inward/record.url?eid=2-s2.0-33847622467&partnerID=MN8TOARS.
    10.1073/pnas.0611173104
  91. Najmudin, S.; Guerreiro, C.I.P.D.; Carvalho, A.L.; Prates, J.A.M.; Correia, M.A.S.; Alves, V.D.; Ferreira, L.M.A.; et al. "Xyloglucan is recognized by carbohydrate-binding modules that interact with ß-glucan chains". Journal of Biological Chemistry 281 13 (2006): 8815-8828. http://www.scopus.com/inward/record.url?eid=2-s2.0-33646840847&partnerID=MN8TOARS.
    10.1074/jbc.M510559200
  92. Brondino, C.D.; Rivas, M.G.; Romão, M.J.; Moura, J.J.G.; Moura, I.. "Structural and electron paramagnetic resonance (EPR) studies of mononuclear molybdenum enzymes from sulfate-reducing bacteria". Accounts of Chemical Research 39 10 (2006): 788-796. http://www.scopus.com/inward/record.url?eid=2-s2.0-33750616404&partnerID=MN8TOARS.
    10.1021/ar050104k
  93. Raaijmakers, H.C.A.; Romão, M.J.. "Formate-reduced E. coli formate dehydrogenase H: The reinterpretation of the crystal structure suggests a new reaction mechanism". Journal of Biological Inorganic Chemistry 11 7 (2006): 849-854. http://www.scopus.com/inward/record.url?eid=2-s2.0-33748337941&partnerID=MN8TOARS.
    10.1007/s00775-006-0129-2
  94. Brondino, C.D.; Romão, M.J.; Moura, I.; Moura, J.J.G.. "Molybdenum and tungsten enzymes: The xanthine oxidase family". Current Opinion in Chemical Biology 10 2 (2006): 109-114. http://www.scopus.com/inward/record.url?eid=2-s2.0-33645243210&partnerID=MN8TOARS.
    10.1016/j.cbpa.2006.01.034
  95. Santos-Silva, T.; Trincão, J.; Carvalho, A.L.; Bonifácio, C.; Auchère, F.; Raleiras, P.; Moura, I.; Moura, J.J.G.; Romão, M.J.. "The first crystal structure of class III superoxide reductase from Treponema pallidum". Journal of Biological Inorganic Chemistry 11 5 (2006): 548-558. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745364810&partnerID=MN8TOARS.
    10.1007/s00775-006-0104-y
  96. Santos-Silva, T.; Trincão, J.; Carvalho, A.L.; Bonifácio, C.; Auchère, F.; Moura, I.; Moura, J.J.G.; Romão, M.J.. "Superoxide reductase from the syphilis spirochete Treponema pallidum: Crystallization and structure determination using soft X-rays". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 61 11 (2005): 967-970. http://www.scopus.com/inward/record.url?eid=2-s2.0-33744487895&partnerID=MN8TOARS.
    10.1107/S174430910502885X
  97. Najmudin, S.; Guerreiro, C.I.P.D.; Ferreira, L.M.A.; Romão, M.J.C.; Fontes, C.M.G.A.; Prates, J.A.M.. "Overexpression, purification and crystallization of the two C-terminal domains of the bifunctional cellulase ctCel9D-Cel44A from Clostridium thermocellum". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 61 12 (2005): 1043-1045. http://www.scopus.com/inward/record.url?eid=2-s2.0-33744503690&partnerID=MN8TOARS.
    10.1107/S1744309105035670
  98. Boer, D.R.; Müller, A.; Fetzner, S.; Lowe, D.J.; Romão, M.J.. "On the purification and preliminary crystallographic analysis of isoquinoline 1-oxidoreductase from Brevundimonas diminuta 7". Acta Crystallographica Section F: Structural Biology and Crystallization Communications 61 1 (2005): 137-140. http://www.scopus.com/inward/record.url?eid=2-s2.0-33645222120&partnerID=MN8TOARS.
    10.1107/S1744309104032105
  99. Carvalho, A.L.; Pires, V.M.R.; Gloster, T.M.; Turkenburg, J.P.; Prates, J.A.M.; Ferreira, L.M.A.; Romão, M.J.; et al. "Insights into the structural determinants of cohesin - Dockerin specificity revealed by the crystal structure of the type II cohesin from Clostridium thermocellum SdbA". Journal of Molecular Biology 349 5 (2005): 909-915. http://www.scopus.com/inward/record.url?eid=2-s2.0-20344372400&partnerID=MN8TOARS.
    10.1016/j.jmb.2005.04.037
  100. Moura, J.J.G.; Brondino, C.D.; Trincão, J.; Romão, M.J.. "Mo and W bis-MGD enzymes: Nitrate reductases and formate dehydrogenases". Journal of Biological Inorganic Chemistry 9 7 (2004): 791-799. http://www.scopus.com/inward/record.url?eid=2-s2.0-8644286154&partnerID=MN8TOARS.
    10.1007/s00775-004-0573-9
  101. Hettmann, T.; Siddiqui, R.A.; Frey, C.; Santos-Silva, T.; Romão, M.J.; Diekmann, S.. "Mutagenesis study on amino acids around the molybdenum centre of the periplasmic nitrate reductase from Ralstonia eutropha". Biochemical and Biophysical Research Communications 320 4 (2004): 1211-1219. http://www.scopus.com/inward/record.url?eid=2-s2.0-3142778495&partnerID=MN8TOARS.
    10.1016/j.bbrc.2004.06.086
  102. Carvalho, A.L.; Goyal, A.; Prates, J.A.M.; Bolam, D.N.; Gilbert, H.J.; Pires, V.M.R.; Ferreira, L.M.A.; et al. "The family 11 carbohydrate-binding module of Clostridium thermocellum Lic26A-Cel5E accommodates ß-1,4- and ß-1,3-1,4-mixed linked glucans at a single binding site". Journal of Biological Chemistry 279 33 (2004): 34785-34793. http://www.scopus.com/inward/record.url?eid=2-s2.0-4544354845&partnerID=MN8TOARS.
    10.1074/jbc.M405867200
  103. Dias, J.M.; Alves, T.; Bonifácio, C.; Pereira, A.S.; Trincão, J.; Bourgeois, D.; Moura, I.; Romão, M.J.. "Structural basis for the mechanism of Ca2+ activation of the di-heme cytochrome c peroxidase from Pseudomonas nautica 617". Structure 12 6 (2004): 961-973. http://www.scopus.com/inward/record.url?eid=2-s2.0-2942613843&partnerID=MN8TOARS.
    10.1016/j.str.2004.03.025
  104. Boer, D.R.; Thapper, A.; Brondino, C.D.; Romão, M.J.; Moura, J.J.G.. "X-ray crystal structure and EPR spectra of "arsenite-inhibited" Desulfovibrio gigas aldehyde dehydrogenase: A member of the xanthine oxidase family". Journal of the American Chemical Society 126 28 (2004): 8614-8615. http://www.scopus.com/inward/record.url?eid=2-s2.0-3242666785&partnerID=MN8TOARS.
    10.1021/ja0490222
  105. Correia Dos Santos, M.M.; Sousa, P.M.P.; Gonçalves, M.L.S.; Romão, M.J.; Moura, I.; Moura, J.J.G.. "Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase". European Journal of Biochemistry 271 7 (2004): 1329-1338. http://www.scopus.com/inward/record.url?eid=2-s2.0-1942535144&partnerID=MN8TOARS.
    10.1111/j.1432-1033.2004.04041.x
  106. Santos-Silva, T.; Dias, J.M.; Bourenkov, G.; Bartunik, H.; Moura, I.; Romão, M.J.. "Crystallization and preliminary X-ray diffraction analysis of the 16-haem cytochrome of Desulfovibrio gigas". Acta Crystallographica Section D: Biological Crystallography 60 5 (2004): 968-970. http://www.scopus.com/inward/record.url?eid=2-s2.0-16644373383&partnerID=MN8TOARS.
    10.1107/S0907444904005621
  107. Hettmann, T.; Siddiqui, R.A.; Von Langen, J.; Frey, C.; Romão, M.J.; Diekmann, S.. "Mutagenesis study on the role of a lysine residue highly conserved in formate dehydrogenases and periplasmic nitrate reductases". Biochemical and Biophysical Research Communications 310 1 (2003): 40-47. http://www.scopus.com/inward/record.url?eid=2-s2.0-0141625736&partnerID=MN8TOARS.
    10.1016/j.bbrc.2003.08.114
  108. Carvalho, A.L.; Dias, F.M.V.; Prates, J.A.M.; Nagy, T.; Gilbert, H.J.; Davies, G.J.; Ferreira, L.M.A.; Romão, M.J.; Fontes, C.M.G.A.. "Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex". Proceedings of the National Academy of Sciences of the United States of America 100 SUPPL. 2 (2003): 13809-13814. http://www.scopus.com/inward/record.url?eid=2-s2.0-0345564859&partnerID=MN8TOARS.
    10.1073/pnas.1936124100
  109. Garattini, E.; Mendel, R.; Romão, M.J.; Wright, R.; Terao, M.. "Mammalian molybdo-flavoenzymes, an expanding family of proteins: Structure, genetics, regulation, function and pathophysiology". Biochemical Journal 372 1 (2003): 15-32. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037954564&partnerID=MN8TOARS.
    10.1042/BJ20030121
  110. Almeida, M.G.; Macieira, S.; Gonçalves, L.L.; Huber, R.; Cunha, C.A.; Romão, M.J.; Costa, C.; et al. "The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774. Re-evaluation of the spectroscopic data and redox properties". European Journal of Biochemistry 270 19 (2003): 3904-3915. http://www.scopus.com/inward/record.url?eid=2-s2.0-0141704108&partnerID=MN8TOARS.
    10.1046/j.1432-1033.2003.03772.x
  111. Bonifácio, C.; Cunha, C.A.; Miller, A.; Timóteo, C.G.; Dias, J.M.; Moura, I.; Romão, M.J.. "Crystallization and preliminary X-ray diffraction analysis of the di-haem cytochrome c peroxidase from Pseudomonas stutzeri". Acta Crystallographica - Section D Biological Crystallography 59 2 (2003): 345-347. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037319850&partnerID=MN8TOARS.
    10.1107/S0907444902020760
  112. Cunha, C.A.; Macieira, S.; Dias, J.M.; Almeida, G.; Gonçalves, L.L.; Costa, C.; Lampreia, J.; et al. "Cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774. The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA)". Journal of Biological Chemistry 278 19 (2003): 17455-17465. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037592981&partnerID=MN8TOARS.
    10.1074/jbc.M211777200
  113. Raaijmakers, H.; Macieira, S.; Dias, J.M.; Teixeira, S.; Bursakov, S.; Huber, R.; Moura, J.J.G; Moura, I.; Romão, M.J.. "Gene sequence and the 1.8 Å crystal structure of the tungsten-containing formate dehydrogenase from Desulfovibrio gigas". Structure 10 9 (2002): 1261-1272. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036711479&partnerID=MN8TOARS.
    10.1016/S0969-2126(02)00826-2
  114. Carvalho, A.L.; Sanz, L.; Barettino, D.; Romero, A.; Calvete, J.J.; Romão, M.J.. "Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: A homologue of human PSA". Journal of Molecular Biology 322 2 (2002): 325-337. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036382902&partnerID=MN8TOARS.
    10.1016/S0022-2836(02)00705-2
  115. Dias, J.M.; Bonifácio, C.; Alves, T.; Moura, J.J.G.; Moura, I.; João Romão, M.. "Crystallization and preliminary X-ray diffraction analysis of two pH-dependent forms of a di-haem cytochrome c peroxidase from Pseudomonas nautica". Acta Crystallographica Section D: Biological Crystallography 58 4 (2002): 697-699. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036005631&partnerID=MN8TOARS.
    10.1107/S0907444902002354
  116. Romão, M.J.; Cunha, C.A.; Brondino, C.D.; Moura, J.J.. "Molybdenum enzymes in reactions involving aldehydes and acids.". Metal ions in biological systems 39 (2002): 539-570. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036358881&partnerID=MN8TOARS.
  117. Pedro Ferreira; N. M. F. S. A. Cerqueira; Catarina Coelho; P. A. Fernandes; Maria João Romão; M. J. Ramos. "New insights about the monomer and homodimer structures of the human AOX1". Physical Chemistry Chemical Physics 21 25 (2001): 13545-13554. https://novaresearch.unl.pt/en/publications/4b79d81a-4a00-4d83-9641-5b7de8a13623.
    Published • 10.1039/c9cp01040h
  118. Rebelo, J.; Dias, J.; Huber, R.; Moura, J.; Romão, M.. "Structure refinement of the aldehyde oxiodoreductase from Desulfovibrio gigas (MOP) at 1.28 Å". Journal of Biological Inorganic Chemistry 6 8 (2001): 791-800. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035161477&partnerID=MN8TOARS.
    10.1007/s007750100255
  119. Carvalho, A.L.; Dias, J.M.; Sanz, L.; Romero, A.; Calvete, J.J.; Romão, M.J.. "Purification, crystallization and identification by X-ray analysis of a prostate kallikrein from horse seminal plasma". Acta Crystallographica Section D: Biological Crystallography 57 8 (2001): 1180-1183. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034897925&partnerID=MN8TOARS.
    10.1107/S0907444901009805
  120. Raaijmakers, H.; Teixeira, S.; Dias, J.M.; Almendra, M.J.; Brondino, C.D.; Moura, I.; Moura, J.J.G.; Romão, M.J.. "Tungsten-containing formate dehydrogenase from Desulfovibrio gigas: Metal identification and preliminary structural data by multi-wavelength crystallography". Journal of Biological Inorganic Chemistry 6 4 (2001): 398-404. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034852815&partnerID=MN8TOARS.
    10.1007/s007750100215
  121. Rebelo, J.; Macieira, S.; Dias, J.M.; Huber, R.; Ascenso, C.S.; Rusnak, F.; Moura, J.J.G.; Moura, I.; Romão, M.J.. "Gene sequence and crystal structure of the aldehyde oxidoreductase from Desulfovibrio desulfuricans ATCC 27774". Journal of Molecular Biology 297 1 (2000): 135-146. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034677657&partnerID=MN8TOARS.
    10.1006/jmbi.2000.3552
  122. Sadeghi, S.J.; Valetti, F.; Cunha, C.A.; Romão, M.J.; Soares, C.M.; Gilardi, G.. "Ionic strength dependence of the non-physiological electron transfer between flavodoxin and cytochrome C553from D. vulgaris". Journal of Biological Inorganic Chemistry 5 6 (2000): 730-737. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033748801&partnerID=MN8TOARS.
    10.1007/s007750000162
  123. Dias, J.M.; Cunha, C.A.; Teixeira, S.; Almeida, G.; Costa, C.; Lampreia, J.; Moura, J.J.G.; Moura, I.; Romão, M.J.. "Crystallization and preliminary X-ray analysis of a membrane-bound nitrite reductase from Desulfovibrio desulfuricans ATCC 27774". Acta Crystallographica Section D: Biological Crystallography 56 2 (2000): 215-217. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034141953&partnerID=MN8TOARS.
    10.1107/S0907444999016066
  124. Duarte, R.O.; Archer, M.; Dias, J.M.; Bursakov, S.; Huber, R.; Moura, I.; Romão, M.J.; Moura, J.J.G.. "Biochemical/spectroscopic characterization and preliminary X-ray analysis of a new aldehyde oxidoreductase isolated from Desulfovibrio desulfuricans ATCC 27774". Biochemical and Biophysical Research Communications 268 3 (2000): 745-749. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034708129&partnerID=MN8TOARS.
    10.1006/bbrc.2000.2135
  125. Cunha, C.A.; Romão, M.J.; Sadeghi, S.J.; Valetti, F.; Gilardi, G.; Soares, C.M.. "Effects of protein-protein interactions on electron transfer: Docking and electron transfer calculations for complexes between flavodoxin and c- type cytochromes". Journal of Biological Inorganic Chemistry 4 3 (1999): 360-374. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032788471&partnerID=MN8TOARS.
    10.1007/s007750050323
  126. Dias, J.M.; Than, M.E.; Humm, A.; Huber, R.; Bourenkov, G.P.; Bartunik, H.D.; Bursakov, S.; et al. "Crystal structure of the first dissimilatory nitrate reductase at 1.9 Å solved by MAD methods". Structure 7 1 (1999): 65-79. http://www.scopus.com/inward/record.url?eid=2-s2.0-0003123613&partnerID=MN8TOARS.
    10.1016/S0969-2126(99)80010-0
  127. Dias, J.M.; Bursakov, S.; Carneiro, C.; Moura, J.J.G.; Moura, I.; Romäo, M.J.. "Crystallization and preliminary X-ray analysis of a nitrate reductase from Desulfovibrio desulfuricans ATCC 27774". Acta Crystallographica Section D: Biological Crystallography 55 4 (1999): 877-879. http://www.scopus.com/inward/record.url?eid=2-s2.0-4243397343&partnerID=MN8TOARS.
    10.1107/S0907444998014735
  128. Archer, M.; Carvalho, A.L.; Teixeira, S.; Moura, I.; Moura, J.J.G.; Rusnak, F.; Romão, M.J.. "Structural studies by X-ray diffraction on metal substituted desulforedoxin, a rubredoxin-type protein". Protein Science 8 7 (1999): 1536-1545. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032805156&partnerID=MN8TOARS.
    10.1110/ps.8.7.1536
  129. Glatigny, A.; Hof, P.; Romão, M.J.; Huber, R.; Scazzocchio, C.. "Altered specificity mutations define residues essential for substrate positioning in xanthine dehydrogenase". Journal of Molecular Biology 278 2 (1998): 431-438. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032079684&partnerID=MN8TOARS.
    10.1006/jmbi.1998.1707
  130. Voityuk, A.A.; Albert, K.; Romão, M.J.; Huber, R.; Rösch, N.. "Substrate Oxidation in the Active Site of Xanthine Oxidase and Related Enzymes. A Model Density Functional Study". Inorganic Chemistry 37 2 (1998): 176-180. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001136357&partnerID=MN8TOARS.
    10.1021/ic9707570
  131. Varela, P.F.; Romero, A.; Sanz, L.; Romão, M.J.; Töpfer-Petersen, E.; Calvete, J.J.. "The 2.4 Å resolution crystal structure of boar seminal plasma PSP-I/PSP-II: A zona pellucida-binding glycoprotein heterodimer of the spermadhesin family built by a CUB domain architecture". Journal of Molecular Biology 274 4 (1997): 635-649. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031565824&partnerID=MN8TOARS.
    10.1006/jmbi.1997.1424
  132. Romão, M.J.; Kölln, I.; Dias, J.M.; Carvalho, A.L.; Romero, A.; Varela, P.F.; Sanz, L.; Töpfer-Petersen, E.; Calvete, J.J.. "Crystal structure of acidic seminal fluid protein (aSFP) at 1.9 Å resolution: A bovine polypeptide of the spermadhesin family". Journal of Molecular Biology 274 4 (1997): 650-660. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031565993&partnerID=MN8TOARS.
    10.1006/jmbi.1997.1423
  133. Romão, M.J.; Rösch, N.; Huber, R.. "The molybdenum site in the xanthine oxidase-related aldehyde oxidoreductase from Desulfovibrio gigas and a catalytic mechanism for this class of enzymes". Journal of Biological Inorganic Chemistry 2 6 (1997): 782-785. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031446005&partnerID=MN8TOARS.
    10.1007/s007750050195
  134. Archer, M.; Banci, L.; Dikaya, E.; Romão, M.J.. "Crystal structure of cytochrome c' from Rhodocyclus gelatinosus and comparison with other cytochromes c'". Journal of Biological Inorganic Chemistry 2 5 (1997): 611-622. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030830925&partnerID=MN8TOARS.
    10.1007/s007750050176
  135. Romão, M.J.; Knäblein, J.; Huber, R.; Moura, J.J.G.. "Structure and function of molybdopterin containing enzymes". Progress in Biophysics and Molecular Biology 68 2-3 (1997): 121-144. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031554726&partnerID=MN8TOARS.
    10.1016/S0079-6107(97)00022-9
  136. Voityuk, A.A.; Albert, K.; Kostlmeier, S.; Nasluzov, V.A.; Neyman, K.M.; Hof, P.; Huber, R.; Romao, M.J.; Rosch, N.. "Prediction of alternative structures of the molybdenum site in the xanthine oxidase-related aldehyde oxido reductase". Journal of the American Chemical Society 119 13 (1997): 3159-3160. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030988323&partnerID=MN8TOARS.
    10.1021/ja963901+
  137. Romão, M.J.; Huber, R.. "Crystal structure and mechanism of the xanthine oxidase-related aldehyde oxido-reductase from D. gigas". Biochemical Society Transactions 25 3 (1997): http://www.scopus.com/inward/record.url?eid=2-s2.0-33745123701&partnerID=MN8TOARS.
  138. Adams, B.; Smith, A.T.; Doyle, W.A.; Bray, R.C.; Ryan, M.; Harrison, R.; Wolstenholme, A.J.; et al. "Expression of wild-type and mutated Drosophila melanogaster xanthine dehydrogenases in Aspergillus nidulans". Biochemical Society Transactions 25 3 (1997): http://www.scopus.com/inward/record.url?eid=2-s2.0-0030764385&partnerID=MN8TOARS.
  139. Romero, A.; Romao, M.J.; Varela, P.F.; Kölln, I.; Dias, J.M.; Carvalho, A.L.; Sanz, L.; Töpfer-Petersen, E.; Calvete, J.J.. "The crystal structures of two spermadhesins reveal the CUB domain fold". Nature Structural Biology 4 10 (1997): 783-788. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031255268&partnerID=MN8TOARS.
    10.1038/nsb1097-783
  140. Romão, M.J.; Huber, R.. "Crystal structure and mechanism of action of the xanthine oxidase-related aldehyde oxidoreductase from Desulfovibrio gigas". Biochemical Society Transactions 25 3 (1997): 755-757. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030761340&partnerID=MN8TOARS.
    10.1042/bst0250755
  141. Dias, J.M.; Carvalho, A.L.; Kölln, I.; Calvete, J.J.; Töpfer-Petersen, E.; Varela, P.F.; Romero, A.; Urbanke, C.; Romão, M.J.. "Crystallization and preliminary X-ray diffraction studies of aSFP, a bovine seminal plasma protein with a single CUB domain architecture". Protein Science 6 3 (1997): 725-727. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030896835&partnerID=MN8TOARS.
    10.1002/pro.5560060323
  142. Huber, R.; Hof, P.; Duarte, R.O.; Moura, J.J.G.; Moura, I.; Liu, M.-Y.; LeGall, J.; et al. "A structure-based catalytic mechanism for the xanthine oxidase family of molybdenum enzymes". Proceedings of the National Academy of Sciences of the United States of America 93 17 (1996): 8846-8851. http://www.scopus.com/inward/record.url?eid=2-s2.0-9444219600&partnerID=MN8TOARS.
    10.1073/pnas.93.17.8846
  143. Zajc, A.; Romão, M.J.; Turk, B.; Huber, R.. "Crystallographic and fluorescence studies of ligand binding to N-carbamoylsarcosine amidohydrolase from Arthrobacter sp.". Journal of Molecular Biology 263 2 (1996): 269-283. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030601891&partnerID=MN8TOARS.
    10.1006/jmbi.1996.0574
  144. Kiefersauer, R.; Stetefeld, J.; Gomis-Rüth, F.X.; Romão, M.J.; Lottspeich, F.; Huber, R.. "Protein-Crystal Density by Volume Measurement and Amino-Acid Analysis". Journal of Applied Crystallography 29 PART 4 (1996): 311-317. http://www.scopus.com/inward/record.url?eid=2-s2.0-0006403784&partnerID=MN8TOARS.
    10.1107/S0021889895014932
  145. Moura, J.J.G.; Goodfellow, B.J.; Romão, M.J.; Rusnak, F.; Moura, I.. "Analysis, design and engineering of simple iron-sulfur proteins: Tales from rubredoxin and desulforedoxin". Comments on Inorganic Chemistry 19 1 (1996): 47-66. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031287732&partnerID=MN8TOARS.
    10.1080/02603599608032727
  146. Goodfellow, B.J.; Tavares, P.; Romão, M.J.; Czaja, C.; Rusnak, F.; LeGall, J.; Moura, I.; Moura, J.J.G.. "The solution structure of desulforedoxin, a simple iron-sulfur protein: An NMR study of the zinc derivative". Journal of Biological Inorganic Chemistry 1 4 (1996): 341-354. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000053742&partnerID=MN8TOARS.
    10.1007/s007750050062
  147. Romero, A.; Caldeira, J.; Legall, J.; Moura, I.; Moura, J.J.G.; Romao, M.J.. "Crystal structure of flavodoxin from Desulfovibrio desulfuricans ATCC 27774 in two oxidation states". European Journal of Biochemistry 239 1 (1996): 190-196. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029953886&partnerID=MN8TOARS.
    10.1111/j.1432-1033.1996.0190u.x
  148. Frazao, C.; Dias, J.M.; Matias, P.M.; Romao, M.J.; Carrondo, M.A.; Hervas, M.; Navarro, J.A.; De La Rosa, M.; Sheldrick, G.M.. "Cytochrome c6 from the green alga Monoraphidium braunii. Crystallization and preliminary diffraction studies". Acta Crystallographica - Section D Biological Crystallography 51 2 (1995): 232-234. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029132455&partnerID=MN8TOARS.
    10.1107/S0907444994005184
  149. Archer, M.; Huber, R.; Tavares, P.; Moura, I.; Moura, J.J.G.; Carrondo, M.A.; Sieker, L.C.; LeGall, J.; Romao, M.J.. "Crystal structure of desulforedoxin from Desulfovibrio gigas determined at 1.8 Å resolution: A novel non-heme iron protein structure". Journal of Molecular Biology 251 5 (1995): 690-702. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029123025&partnerID=MN8TOARS.
    10.1006/jmbi.1995.0465
  150. Romão, M.J.; Archer, M.; Moura, I.; Moura, J.J.G.; LeGall, J.; Engh, R.; Schneider, M.; Hof, P.; Huber, R.. "Crystal structure of the xanthine oxidase-related aldehyde oxido-reductase from D. gigas". Science 270 5239 (1995): 1170-1176. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028807552&partnerID=MN8TOARS.
  151. THOENES, U.; FLORES, O.L.; NEVES, A.; DEVREESE, B.; VAN BEEUMEN, J.J.; HUBER, R.; ROMÃO, M.J.; et al. "Molecular cloning and sequence analysis of the gene of the molybdenum-containing aldehyde oxido-reductase of Desulfovibrio gigas The deduced amino acid sequence shows similarity to xanthine dehydrogenase". European Journal of Biochemistry 220 3 (1994): 901-910. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028280650&partnerID=MN8TOARS.
    10.1111/j.1432-1033.1994.tb18693.x
  152. de C.T. Carrondo, M.A.A.F.; Morais, J.; Romão, C.C.; Romão, M.J.; Veiros, L.F.. "Cationic derivatives of niobocene(IV). Crystal structures of [Cp2NbL2][BF4]2 (L = CNMe, NCMe)". Polyhedron 12 7 (1993): 765-770. http://www.scopus.com/inward/record.url?eid=2-s2.0-0043010607&partnerID=MN8TOARS.
    10.1016/S0277-5387(00)81757-X
  153. ROMÃO, M.J.; BARATA, B.A.S.; ARCHER, M.; LOBECK, K.; MOURA, I.; CARRONDO, M.A.; LeGALL, J.; et al. "Subunit composition, crystallization and preliminary crystallographic studies of the Desulfovibrio gigas aldehyde oxidoreductase containing molybdenum and [2Fe-2S] centers". European Journal of Biochemistry 215 3 (1993): 729-732. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027217139&partnerID=MN8TOARS.
    10.1111/j.1432-1033.1993.tb18085.x
  154. Romão, M.J.; Turk, D.; Gomis-Rüth, F.-X.; Huber, R.; Schumacher, G.; Möllering, H.; Rüssmann, L.. "Crystal structure analysis, refinement and enzymatic reaction mechanism of N-carbamoylsarcosine amidohydrolase from Arthrobacter sp. at 2·0 Å resolution". Journal of Molecular Biology 226 4 (1992): 1111-1130. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026483887&partnerID=MN8TOARS.
    10.1016/0022-2836(92)91056-U
  155. Laber, B.; Gomis-Ruth, F.-X.; Romao, M.J.; Huber, R.. "Escherichia coli dihydrodipicolinate synthase. Identification of the active site and crystallization". Biochemical Journal 288 2 (1992): 691-695. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026486643&partnerID=MN8TOARS.
    10.1042/bj2880691
  156. Mynott, R.; Romão, M.J.; Herold, B.J.. "13C NMR study of substituted 10,10-diphenyl-9-phenanthrones. Structure determination and signal assignment using 2D NMR techniques". Magnetic Resonance in Chemistry 27 12 (1989): 1130-1133. http://www.scopus.com/inward/record.url?eid=2-s2.0-84989143773&partnerID=MN8TOARS.
    10.1002/mrc.1260271205
  157. Angermund, H.; Grevels, F.-W.; Moser, R.; Benn, R.; Krüger, C.; Romão, M.J.. "(¿2-trans-Cyclooctene)2Fe(CO)3 and related complexes: Structure and dynamic behavior". Organometallics 7 9 (1988): 1994-2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-0001534489&partnerID=MN8TOARS.
    10.1021/om00099a015
  158. Herold, B.J.; Romão, M.J.; Empis, J.M.A.; Evans, J.C.; Rowlands, C.C.. "The use of electron spin resonance and ENDOR and TRIPLE resonance methods for structural elucidation". Journal of the Chemical Society, Faraday Transactions 1: Physical Chemistry in Condensed Phases 83 1 (1987): 43-49. http://www.scopus.com/inward/record.url?eid=2-s2.0-37049074188&partnerID=MN8TOARS.
    10.1039/F19878300043
  159. KRUGER, C; ROMAO, MJ. "STRUCTURE OF 6-FLUORO-10,10'-DIPHENYL-9-PHENANTHRONE (1), 7-CHLORO-10,10'-DIPHENYL-9-PHENANTHRONE (2) AND 3-METHOXY-10,10'-DIPHENYL-9-PHENANTHRONE (3)". Acta Crystallographica Section C-Crystal Structure Communications 42 (1986): 1404-1408.
    10.1107/S0108270186092107
  160. Benn, R.; Holle, S.; Jolly, P.W.; Krüger, C.; Romão, C.C.; Romão, M.J.; Rufinska, A.; Schroth, G.. "¿3-allyl complexes of molybdenum: reactions of [MoCl(¿3-C3H5)3] 2 and the crystal structure of [MoOAc(¿3-C3H5)3]". Polyhedron 5 1-2 (1986): 461-471. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000519387&partnerID=MN8TOARS.
    10.1016/S0277-5387(00)84950-5
  161. Frühauf, H.-W.; Sails, F.; Goddard, R.J.; Romão, M.J.. "1,5-Dihydropyrrol-2-ones from (1,4-Diaza-1,3-diene )tricarbonyliron and Alkyne. 2. Structure of a [2.2.2] Bicyclic Intermediate with Iron at the Bridgehead Position". Organometallics 4 5 (1985): 948-949. http://www.scopus.com/inward/record.url?eid=2-s2.0-0000812375&partnerID=MN8TOARS.
    10.1021/om00124a031
  162. Brauer, D.J.; Hietkamp, S.; Sommer, H.; Stelzer, O.; Müller, G.; Romão, M.J.; Krüger, C.. "Lineare oligophosphaalkane XIII. Triphosphaalkane mit demPCPCP-donorskelett; liganden für den aufbau von mehrkernkomplexen". Journal of Organometallic Chemistry 296 3 (1985): 411-433. http://www.scopus.com/inward/record.url?eid=2-s2.0-46549094714&partnerID=MN8TOARS.
    10.1016/0022-328X(85)80372-7
  163. A. Dohring; P. W. Jolly; C. Kruger; Maria João Romão. "THE NI(O)-CO2 SYSTEM - STRUCTURE AND REACTIONS OF [NI(PCY3)2(ETA-2-CO2)]". Zeitschrift Fur Naturforschung Section B-A Journal Of Chemical Sciences 40 4 (1985): 484-488. https://novaresearch.unl.pt/en/publications/5a482b20-a899-4dbd-b89d-506350fc9d5e.
  164. HOBERG, H; FANANAS, FJ; ANGERMUND, K; KRUGER, C; ROMAO, MJ. "STRUCTURES AND PROPERTIES OF NEW CARBAMOYL-NICKEL COMPLEXES". Journal of Organometallic Chemistry 281 2-3 (1985): 379-388.
    10.1016/0022-328X(85)87123-0
  165. BARLUENGA, J; JOGLAR, J; FUSTERO, S; GOTOR, V; KRUGER, C; ROMAO, MJ. "PREPARATION AND REACTIVITY OF 2-AZA-1,3-BUTADIENES - A DIELS-ALDER ROUTE TO 5,6-DIHYDRO-2H-1,3-OXAZINE DERIVATIVES". Chemische Berichte-Recueil 118 9 (1985): 3652-3663.
    10.1002/cber.19851180919
  166. H. Hoberg; K. Radine; C. Kruger; Maria João Romão. "SYNTHESIS OF NEW PHOSPHINE NICKEL(I) COMPLEXES AND CRYSTAL-STRUCTURE OF MU-3-IODO-TRIS-MU-IODO-CYCLOTRIS(TRIPHENYLPHOSPHINE NICKEL), (TPP)3NI3I4". Zeitschrift Fur Naturforschung Section B-A Journal Of Chemical Sciences 40 5 (1985): 607-614. https://novaresearch.unl.pt/en/publications/caa3ca3c-78e5-4481-95db-b31d7170d30b.
  167. J. J. Eisc; A. M. Piotrowski; A. A. Aradi; C. Kruger; Maria João Romão. "ORGANIC-CHEMISTRY OF LOW-VALENCE TRANSITION-METAL COMPLEXES .10. OXIDATIVE ADDITION OF NICKEL (0) COMPLEXES TO CARBON-CARBON BONDS IN CYCLOBUTADIENE - THE QUESTION OF THE INTERCONVERTABILITY OF CYCLOBUTADIENE-NICKEL (0) COMPLEXES AND THE NICKELARING SYSTEMS". Zeitschrift Fur Naturforschung Section B-A Journal Of Chemical Sciences 40 5 (1985): 624-635. https://novaresearch.unl.pt/en/publications/31069929-7dcf-478d-bd69-5db9172b59b9.
  168. K. R. Porschke; R. Mynott; C. Kruger; Maria João Romão. "TRIS(PHOSPHANE)-NICKEL(0)-ETHENE COMPLEXES (DMPE)(PR3)NI(C2H4) - MOLECULAR-STRUCTURE OF (DMPE)(P03)NI(C2H4)". Zeitschrift Fur Naturforschung Section B-A Journal Of Chemical Sciences 39 8 (1984): 1076-1081. https://novaresearch.unl.pt/en/publications/b043eeb2-84d8-49ac-8db3-994fd2212f8f.
  169. HOBERG, H; SCHAEFER, D; BURKHART, G; KRUGER, C; ROMAO, MJ. "NICKEL(0)-INDUCED C-C COUPLING BETWEEN CARBON-DIOXIDE AND ALKYNES AS WELL AS ALKENES". Journal of Organometallic Chemistry 266 2 (1984): 203-224.
    10.1016/0022-328X(84)80129-1
  170. JOLLY, PW; KRUGER, C; ROMAO, CC; ROMAO, MJ. "ETA-3-ALLYL COMPLEXES OF MOLYBDENUM - THE PREPARATION AND STRUCTURE OF [MO(ETA-3-C3H5)2(ETA-5-C5H5)]". Organometallics 3 6 (1984): 936-937.
    10.1021/om00084a021
  171. Frühauf, H.-W.; Seils, F.; Romao, M.J.; Goddard, R.J.. "Zur Aktivität koordinierter 1,4-Diaza-1,3-diene (dad) bei C¿C-Verknüpfungsreaktionen: 3-Pyrrolin-2-one aus (dad)Fe(CO)3 und Dimethyl-acetylendicarboxylat". Angewandte Chemie International Edition in English 22 12 S (1983): 1435-1450. http://www.scopus.com/inward/record.url?eid=2-s2.0-84912873347&partnerID=MN8TOARS.
    10.1002/anie.198314350
  172. FRUHAUF, HW; SEILS, F; ROMAO, MJ; GODDARD, RJ. "ACTIVITY OF COORDINATED 1,4-DIAZA-1,3-DIENES (DAD) IN C-C BOND FORMING REACTIONS - 1,5-DIHYDROPYRROL-2-ONES FROM (DAD)FE(CO)3 AND DIMETHYL ACETYLENEDICARBOXYLATE". Angewandte Chemie-International Edition in English 22 12 (1983): 992-993.
    10.1002/anie.198309921
Preprint
  1. Guilherme Vilela-Alves; Rita R. Manuel; Aldino Viegas; Philippe Carpentier; Frederic Biaso; Bruno Guigliarelli; Inês A. C. Pereira; Maria João Romão; Cristiano Mota. "Substrate-dependent oxidative inactivation of a W-dependent formate dehydrogenase involving selenocysteine displacement". 2024. https://doi.org/10.1101/2024.01.10.571421.
    10.1101/2024.01.10.571421

Other

Other output
  1. Unravelling Clostridium thermocellum LysM domains. Structural basis for the recognition of chitin and peptidoglycan. 2020. Diana de Oliveira Ribeiro; R. Costa; Benedita Pinheiro; J. L. A. Brás; L. M. Silva; Y. B. Zhang; Sergio Raposo Filipe; et al. https://novaresearch.unl.pt/en/publications/95ad56ac-8e5f-4994-a599-64ea0245b887.
  2. Magnetic crystallization proof-of-concept: Lysozyme and trypsin case study. 2018. Raquel dos Santos; Iana Lychko; Maria João Romão; Roque, A.C.A.; Ana Luísa Carvalho. https://novaresearch.unl.pt/en/publications/88281577-12cd-4b26-8c2c-b811d98f5054.
    10.1107/S2053273318092458
  3. Structural insights on aldehyde oxidase and xanthine oxidoreductase and their roles in xenobiotic metabolism. 2018. Maria João Romão. https://novaresearch.unl.pt/en/publications/54fbcf13-f39a-45c1-817d-8d70714d3e90.
    10.1016/j.freeradbiomed.2018.04.555
  4. The crystal structure of human aldehyde oxidase. 2018. Maria João Romão. https://novaresearch.unl.pt/en/publications/e2b85ca0-927b-4349-83b6-97a123ae0b95.
  5. The Crystal Structure of the R280K Mutant of Human p53 Explains the Loss of DNA Binding. The p53 tumor suppressor is widely found to be mutated in human cancer. This protein is regarded as a molecular hub regulating different cell responses, namely cell death. Compelling data have demonstrated that the impairment of p53 activity correlates with tumor development and maintenance. For these reasons, the reactivation of p53 function is regarded as a promising strategy to halt cancer. In. 2018. Gomes, Ana Sara; Trovão, Filipa; Andrade Pinheiro, Benedita; Freire, Filipe; Gomes, Sara; Oliveira, Carla; Domingues, Lucília; et al. http://hdl.handle.net/1822/54422.
    10.3390/ijms19041184
  6. An integrative strategy to decipher glycan recognition in the human gut microbiome. 2016. Viviana G. Correia; Joana L. A. Bras; Yan Liu; Lisete Silva; Yibing Zhang; Benedita Pinheiro; Maria João Romão; et al. https://novaresearch.unl.pt/en/publications/56c19fd5-923c-4d8b-b240-3ea4dc282361.
  7. The Structural Basis of the Recognition of Di-glucosylated N-glycans by the ER Lectin Malectin. Glycobiology. 2013. Ana Luísa Carvalho; Maria João Romão; Palma, Angelina S.; Pinheiro, Benedita; Liu, Yan; Takeda, Yoichi; Chai, Wengang; et al. https://novaresearch.unl.pt/en/publications/a3f0fd44-8cef-4765-a321-f549d44a6fa4.
  8. Unraveling new functions and modes of action of molybdenum-dependent enzymes. 2013. Maria João Romão. https://novaresearch.unl.pt/en/publications/cd76c58a-66e3-4859-92e2-ed7151dc02a6.
  9. Vanadium compounds as prospective therapeutics: X-ray structure of protein adducts. 2013. Teresa Sacadura Santos-silva; Maria João Romão. https://novaresearch.unl.pt/en/publications/1c00769b-51ef-4386-8dc6-b2766ff916cd.
  10. Determinants of selective group reduction in the TNT-bound xenobiotic reductase B from P. putida. 2013. Ana Luísa Carvalho; Maria João Romão. https://novaresearch.unl.pt/en/publications/02c08dc6-e8b3-4559-9df0-dce85ceb47f3.
  11. Purification, crystallization and preliminary X-ray diffraction analysis of adenosine triphosphate sulfurylase (ATPS) from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774. Native zinc/cobalt-containing ATP sulfurylase (ATPS; EC 2.7.7.4; MgATP: sulfate adenylyltransferase) from Desulfovibrio desulfuricans ATCC 27774 was purified to homogeneity and crystallized. The orthorhombic crystals diffracted to beyond 2.5 A ° resolution and the X-ray data collected should allow the determination of the structure of the zinc-bound form of this ATPS. Although previous biochemical. 2008. Gavel, Olga Yu.; Kladova, Anna V.; Bursakov, Sergey A.; Dias, João M.; Texeira, Susana; Moura, José J. G.; Moura, Isabel; et al. http://hdl.handle.net/10362/7027.
    10.1107/S1744309108008816
  12. The human carnitine acylcarnitine translocase (hCACT): Strategies for its heterologous expression, purification and crystallization. 2007. F. V. Ventura; S. Violante; C. Gomes; Ana Luísa Carvalho; Maria João Romão; M. M. Gaspar; M. E. M. Cruz; et al. https://novaresearch.unl.pt/en/publications/fe3909e5-9fbe-4571-8ae9-cebe17bdfe53.
  13. Structure and function of PKD-CBM44 and CBM30 modules of the bifunctionalClostridium thermocellumcellulase, CtCel9D-Cel44A. 2006. Shabir Najmudin; Ana Luísa Carvalho; Maria João Romão; Catarina I. P. D. Guerreiro; José A. M. Prates; Márcia A. S. Correia; Vitor Alves; et al. https://novaresearch.unl.pt/en/publications/10d48655-f5a8-4263-96a1-2cd568899964.
    10.1107/s0108767306097224
  14. Structure of a Sixteen Heme Cytochrome by X-Ray Crystallography.. 2004. Teresa Santos-Silva; J. M. Dias; G. Bourenkov; H. Bartunik; I. Moura; Maria João Romão. https://novaresearch.unl.pt/en/publications/f3f24d1a-d132-4727-95c9-e41d5d322543.
    10.1107/S0108767304096898
  15. Molecular aspects of denitrification/nitrate dissimilation. 2003. I. Moura; I. Cabrito; G. Almeida; C. Cunha; Maria João Romão; José J. G. Moura. https://novaresearch.unl.pt/en/publications/3f5474db-89f9-4814-adf2-4c085a0912e6.
    10.1016/S0162-0134(03)80717-1
  16. Cytochrome c nitrite reductase from desulfovibrio desulfuricans ATCC 27774. The gene encoding cytochrome c nitrite reductase(NrfA) from Desulfovibrio desulfuricans ATCC 27774 was sequenced and the crystal structure of the enzyme was determined to 2.3-Å resolution. In comparison with homologous structures, it presents structural differences mainly located at the regions surrounding the putative substrate inlet and product outlet, and includes a well defined second calcium. 2003. Moura, Isabel; Cunha, Carlos A.; Macieira, Sofia; Dias, João M.; Almeida, Gabriela Machado de; Gonçalves, Luisa L.; Costa, Cristina; et al. http://hdl.handle.net/10362/1658.
  17. Structural studies on Desulfovibrio desulfuricans ATCC 27774 multiheme nitrite reductase - characterization of the subunits. 2001. L. M. L. Gonçalves; C. Cunha; G. Almeida; S Macieira; Cristina Costa; J. Lampreia; Maria João Romão; José J. G. Moura; I. Moura. https://novaresearch.unl.pt/en/publications/d3ba956e-f04e-4e0e-b3a9-743e74e55eef.
  18. Crystallographic studies on a tungsten-containning formate dehydrogenase from Desulfovibrio gigas. 1999. S. Teixeira; Joao ML Dias; Ana Luísa Carvalho; G. Bourenkov; H. Bartunik; M. J. Almendra; I. Moura; José J. G. Moura; Maria João Romão. https://novaresearch.unl.pt/en/publications/f74c8bb6-8e34-487f-9cb5-290adf5ff4f3.
  19. Cross immunological reactions and spectroscopy study within nitrate reductase and other mononuclear Mo containing enzymes of the sulfate reducing bacteria. 1999. S. A. Bursakov; C. Brondino; J. M. Dias; C. Carneiro; J. Caldeira; R. O. Duarte; Maria João Romão; I. Moura; José J. G. Moura. https://novaresearch.unl.pt/en/publications/83190245-7b86-40d1-bc04-216d0efa9bb2.
  20. Preliminary crystallographic studies of xanthine oxidase purified from rat liver. 1999. Maria João Romão; Ana Luísa Carvalho; J. M. Dias; S. Teixeira; G. Bourenkov; H. Bartunik; R. Huber; Luisa Maia; L. Mira. https://novaresearch.unl.pt/en/publications/02d2e607-791a-4d09-bbdf-cfb00aeb5ec5.
  21. Crystallographic studies of a dissimilatory nitrate reductase and mechanistic implications. 1999. J. M. Dias; M. E. Than; R. Huber; G. P. Bourenkov; H. D. Bartunik; S. Bursakov; José J. G. Moura; I. Moura; Maria João Romão. https://novaresearch.unl.pt/en/publications/87b02097-81ad-4b2c-8c24-452d81a9f67b.
  22. Crystallographic Studies on Xanthine Oxidase Purified From Rat Liver. 1999. Ana Luísa Carvalho; J. M. Dias; S. Teixeira; G. Bourenkov; H. Bartunik; R. Huber; Luisa Maia; L. Mira; Maria João Romão. https://novaresearch.unl.pt/en/publications/de817b39-0015-4780-97e7-4e13be49caf0.
  23. REFINEMENT OF THE ALDEHYDE OXIDO-REDUCTASE FROM DESULFOVIBRIO GIGAS (MOP) AT 1.28 angstrom. 1999. J. M. Rebelo; J. M. Dias; Maria João Romão; G. P. Bourenkov; H. D. Bartunick; R. Huber. https://novaresearch.unl.pt/en/publications/24f70b72-0f9c-4b86-ad3c-31bc48ac1bde.
  24. Crystallographic Studies of D.gigas Formate Dehydrogenase.. 1999. S. Teixeira; J. M. Dias; A. L. Carvalho; M. J. Almendra; I. Moura; José J. G. Moura; Maria João Romão; G. Bourenkov; H. Bartunik. https://novaresearch.unl.pt/en/publications/3386cd4b-33b2-44cc-a4c0-12bbc5d942d0.
  25. X-ray Studies of a Nitrite Reductase. Preliminary Results. 1999. C. A. Cunha; J. M. Dias; G Almeida; C Costa; José J. G. Moura; I. Moura; Maria João Romão; A. Thompson. https://novaresearch.unl.pt/en/publications/beddf3e7-3047-4ab1-8f21-f0e860eabd07.
  26. The three-dimensional structure of mammalian spermadhesins determined by x-ray crystallography. 1997. A. Romero; P. F. Varela; Maria João Romão; L. Sanz; E. TopferPetersen; J. J. Calvete. https://novaresearch.unl.pt/en/publications/a1d0430a-e126-4db0-a46e-df9e73e7abd0.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/01 Biological reduction of CO2 to formate by a W-dependent Formate dehydrogenase 6th Symposium on Advanced Biological Inorganic Chemistry 7-11 January, Kalkota
(KOLKATA, India)
2021/09 Integrative Structural Biology beyond 2021 Internal Seminar Series
UCIBIO - NOVA University Lisbon
2019 Mo/W-dependent Formate Dehydrogenases and the reversible interconversion of CO2 and formate (PLENARY) 2019- International Conference on Hydrogenases
(Lisboa, Portugal)
2019 What crystallography can still reveal about two families of Mo and W enzymes 11th MOLYBDENUM & TUNGSTEN ENZYME CONFERENCE- MOTEC
(Potsdam, Germany)
2018 Structural insights on Aldehyde Oxidase and Xanthine Oxidoreductase and their roles in xenobiotic metabolism 19th SFRRI Biennial Meeting, Lisbon, 4-7 June 2018
(Lisboa, Portugal)
2017 Structure and function of mammalian Aldehyde Oxidases and their role in the metabolism of drugs and xenobiotics 14th ISABC
(Toulouse, France)
2017 Mammalian Aldehyde Oxidases and their impact in xenobiotics metabolism and drug development, Santa Fe, New Mexico, USA, June 18-23 2017 10th MOTEC- Mo and W Enzyme Conference
( Santa Fe, New Mexico, United States)
2017 The Crystal Structure of human Aldehyde Oxidase 21st North American ISSX Meeting, Providence, Rhode Island
(Providence, Rhode Island, United States)
2016 Molybdenum enzymes in Drug Metabolism and Biotransformations , 2016- ICCC2016
(Brest, France)
2015 “Aldehyde Oxidases in Drug Metabolism and Biotransformations”. (PLENARY) 9th MOTEC - Molybdenum and Tungsten Molybdenum Conference,
(Balatonfüred, Hungary)
2014 “Molybdenum Enzymes in Health and Disease” 2014 - FrenchBIC Meeting
(Anglet, France)
2013 “Unraveling new functions and modes of action of molybdenum-dependent enzymes” (PLENARY) 2013 - 9th European Biophysics Congress EBSA2013,
(Lisboa, Portugal)
2011 The Crystal Structure of Mouse Aldehyde Oxidase Homologue 1 and Insights into Substrate Specificity MOTEC - MOLYBDENUM AND TUNGSTEN ENZYME MEETING , Alberta, Canada, August 2011,
(Alberta, Canada)
2007 “Molecular and cellular biology of Mo and W" (5th GRC Mo&W enzymes) 5th GORDON Research Conference on “Molybdenum & Tungsten Enzymes”, New-London, NH, USA, July, 2007
(New-London, NH, United States)
2006 "The Role of Molybdenum in Biology" 9th International Symposia on Metal Ions in Biology and Medicine
(Lisboa, Portugal)
2005 Penta-heme Nitrite Reductase and di-heme Cytochrome c Peroxidase International Conference in Bioinorganic Chemistry ICBIC
(Ann Arbor, United States)
2005 Structural Studies on Formate Dehydrogenases (4th GRC on Mo&W enzymes) 4th GORDON Research Conference on “Molybdenum and tungsten Enzymes”,
(Oxford, United Kingdom)
2004 “Structural Studies in active and inactive forms of di-heme cytochrome c peroxidases from Pseudomonas nautica and Pseudomonas stutzeri” 36th International Congress in Coordination Chemistry
(Merida, Mexico)
2003 A Crystallographic Perspective of Molybdenum in Biology (PLENARY) FIGIPS 2003
(Lisboa, Portugal)
2002 How crystallography contributed to the understanding of Mo and W enzymes Alumni Meeting in honor to Robert Huber
(Ringberg, Germany)
2002 W-FORMATE DEHYDROGENASE And NITRATE REDUCTASE From SULFATE REDUCERS: Structures and Functions from Enzymes of the Same Family EUROBIC-6, Lund Copenhagen
(Copenhagen, Denmark)
2002 Molybdopterin-Dependent Enzymes And The Role Of The Catalytic Mo (or W) Active Site (PLENARY) SFB Congress- Metal Mediated Reactions Modelled after Nature
(Jena, Germany)
2001 “Formate Dehydrogenase From Desulfovibrio gigas : A Tungsten Enzyme Containing Se And 4x[4F-4S] Centers” (2nd GRC on Mo&W enzymes) 2nd GORDON Research Conference on Mo and W proteins
GRC (Oxford, United Kingdom)
2001 Crystallographic Studies And Mechanistic Implications On Mo And W Enzymes 20th European Crystallographic Meeting
(Krakow, Poland)
1999 Structural Studies on Molybdopterin containing Enzymes Invited guest to the Institut für Biochemie, Universidade de Medicina de Lübeck
Institut für Biochemie, Universidade de Medicina de Lübeck, Germany (Lübeck, Germany)
1999 “The first crystal structure of a dissimilatory Nitrate Reductase and implications on the reactions mechanism” (1st GRC on Mo&W enzymes) 1st GORDON Research Conference on Molybdenum and Tungsten Enzymes
GRC (Plymouth, New Hampshire, United States)
1998 Structure and Functional Aspects of Molybdopterin containing enzymes TMR Summer School I and II
Instituto de Tecnologia Quimica e Biologica (Oeiras, Portugal)
1998 Crystallography of Mo-Hydroxylases European Xanthine Oxidase Workshop
(Roterdam, Netherlands)

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2023/09/01 - Current Structural, dynamics and mechanistic studies of two enzymatic systems with environmental and biotechnological Impact
Co-supervisor of Guilherme Vilela Alves
Doctoral Program in Biochemistry (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

UCIBIO- Unidade de Ciências Biomoleculares Aplicadas, Portugal
2023/02/01 - Current A novel generation of DNA polymerases for qPCR molecular diagnostics
Co-supervisor of Hélvio Miguel Rodrigues Simões
PhD (PhD)
Universidade Nova de Lisboa Departamento de Química, Portugal
2019/12/02 - Current Biointeration studies of membrane-bound catechol-O-methyltransferase polymorphic variants with commercial Parkinson’s inhibitors
Co-supervisor of Ana Margarida Grilo Gonçalves
Biochemistry (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

UCIBIO- Unidade de Ciências Biomoleculares Aplicadas, Portugal
2019 - Current Biosynthesis and a characterizations of an antibody fragment against STEAPI as a vehicle for cancer therapy
Co-supervisor of Ana Margarida Grilo Gonçalves
Biomedicina (PhD)
Universidade da Beira Interior, Portugal
2016 - 2022 Peptidoglycan amidation in Gram-positive bacteria and structural studies in the Staphylococcus aureus enzymes
Co-supervisor of Francisco Leisico
Biomolecular Sciences (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2013 - 2017 Structural studies on molybdenum-dependent enzymes: from transporters to enzymes
Co-supervisor of Ana Rita Castro Otrelo Cardoso
Bioquímica (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2012 - 2016 Structural and Functional studies on the Reactivity of CORMs with plasma proteins
Co-supervisor of Marino Filipe Alves dos Santos
Bioquímica (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2008 - 2014 Crystallographic studies of proteins involved in the mRNA Localization Mechanisms in Drosophila melanogaster and Amidation of the Peptidoglycan Residues in Staphylococcus aureus
Co-supervisor of Diana Maria Patacas Viegas Vieira
Bioquímica (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2009 - 2012 Integrated study by NMR and X-ray Crystallography on the analysis of the molecular interactions in heme-binding proteins
Co-supervisor of Filipe Miguel dos Santos Freire
Bioquímica (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2008 - 2011 Crystallographic Studies on Molybdopterin — dependent Enzymes
Supervisor of Catarina Maria Campos Coelho
Bioquímica (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2002 - 2006 Metabolismo do Metilglioxal em Tripanossomatídeos
Co-supervisor of Lídia Isabel Sebastião Barata
Bioquímica
Universidade de Lisboa, Portugal
2002 - 2006 Structural Characterization of Heme and Non-heme Iron Containing Proteins
Supervisor of Teresa Sacadura Santos Silva
Bioquímica (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2000 - 2004 Biosynthesis and Function of Biological Pteridines. Structural Studies on Two Molybdopterin Containing Aldehyde Oxido-reductases, from Desulfovibrio Desulfuricans Atcc 27774 and from Desulfovibrio Gigas, and the Gtp Cyclohydrolase I on E. Coli, Responsibl
Co-supervisor of Jorge Manuel Baeta Simões Rebelo
Biochemistry (PhD)
Universidade Nova de Lisboa, Portugal
1999 - 2002 Structure & Function of Nitrate Reductase and Di-haem Cytochrome C Peroxidase
Supervisor of João Miguel Carrapa Nunes Dias
Bioquímica (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
1998 - 2002 Caracterização Estrutural de Proteínas Envolvidas em Processos de Reprodução Animal
Supervisor of Ana Luísa Moreira de Carvalho
Bioquímica (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
1998 - 2002 Modelação Molecular e Caracterização Estrutural de Quimeras de Proteínas de Transferência Electrónica
Co-supervisor of Carlos Manuel Alves da Costa Cunha
Bioquímica (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
1993 - 1997 Crystal Structure Analysis of Metalloproteins: Cytochrome C´ and Desulforedoxin (native and Metal Reconstituted)
Supervisor of Margarida Archer Baltazar Pereira Silva Franco Frazão
Bioquímica (PhD)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Event organisation

Event name
Type of event (Role)
Institution / Organization
2011 - Current 9TH CARBOHYDRATE BIOENGINEERING MEETING CBM9, May 2011, Lisbon (2011)
Conference (Member of the Organising Committee)
2006 - Current EUROBIC 2006 8th EUROPEAN BIOLOGICAL CHEMISTRY CONFERENCE, Aveiro (Organizing Committee) (>500 participants). (2006)
Congress (Member of the Organising Committee)
2006 - Current 6th IBERO-AMERICAN CONGRESS OF BIOPHYSICS (2006)
Congress (Member of the Scientific Committee)
1998 - Current SUMMER SCHOOL I “STRUCTURE AND FUNCTION OF METALLOPROTEINS”, ITQB, Oeiras (Organizing Committee) (ca. 80 participants). (1997)
Workshop (Member of the Organising Committee)
1997 - Current ECM 17 -EUROPEAN CRYSTALLOGRAPHIC MEETING (>900 participants). IST, Lisboa (Organizing Committee) (1997)
Congress (Member of the Organising Committee)

Jury of academic degree

Topic
Role
Candidate name (Type of degree)
Institution / Organization
2018 “Purification and Characterization of Mouse Aldehyde Oxidases”
Thesis Member
Gokhan Kucukgoze (PhD)
Universität Potsdam Institut für Biochemie und Biologie, Germany
2013 “Die Funktion und Spezifität der Molybdän-Cofaktor-bindenden Chaperone für die Formiat-Dehydrogenasen aus Escherichia coli und Rhodobacter capsulatus”
Thesis Member
By Nadine Schwanhold (born Böhmer) (PhD)
Universität Potsdam Institut für Biochemie und Biologie, Germany
2012 Biosynthesis isolation and kinetic characterization of recombinant human catechol-O-methyltransferase from pichia pastoris strains
(Thesis) Main arguer
Augusto Quaresma Henriques Pedro (PhD)
Universidade da Beira Interior, Portugal
2009 New Insights Into the Structure and Function of Human Phenylalanine Hidroxylase: Interallelic Complementation Protein Misfolding and Mechanism of Catalytic Activation
(Thesis) Main arguer
João Paulo Travassos Leandro (PhD)
Universidade de Lisboa, Portugal
2005 Structural and Functional Insights Into the Role of Carbohydrate Esterases and Carbohydrate-binding Modules in Plant Cell Wall Hydrolysis
(Thesis) Main arguer
Márcia Alexandra da Silva Correia (PhD)
2003 X-ray Structure Determination of Iron-containing Proteins from Desulfovibrio Desulfuricans Atcc 27774 in Different Redox States: Bacterioferritin and Hybrid Cluster Protein
(Thesis) Main arguer
Sofia Isabel Vaz Queiroz de Macedo (PhD)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2002 Proteínas Contendo Molibdénio e Tungsténio
(Thesis) Main arguer
Rui Manuel de Oliveira Duarte (PhD)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2000 Nmr Solution Structure of Cytochromes C3: Insight Into the Structural Origins of Functional Cooperativities (main Arguer)
(Thesis) Main arguer
Ana Cristina Messias Coelho (PhD)
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1996 Cristalização e Análise Estrutural por Difracção de Raios X de Metaloproteínas Bacterianas
(Thesis) Main arguer
José António Morais Antunes (PhD)

Conference scientific committee

Conference name Conference host
2022/07 - 2022/07 25th IUBMB, 46th FEBS, 15th PABMB Congress, Lisbon (symposium co-chair) IUBMB-FEBS-PABMB Congress
2018 - 2018 ECM 31, European Crystallographic Meeting , Oviedo Spain (Session Chair)
2010 - 2010 IV SPANISH-PORTUGUESE BIOPHYSICAL CONGRESS (Session Chair)
2009 - 2009 6th GORDON RESEARCH CONFERENCE on “Molybdenum & Tungsten Enzymes”, (Chair) GRC Conferences
2007/07/01 - 2007/07/06 7th GORDON RESEARCH CONFERENCE on Molybdenum & Tungsten Enzymes (Vice-Chair) Colby-Sawyer College 541 Main Street New London, NH, United States

Evaluation committee

Activity description
Role
Institution / Organization Funding entity
2019 - Current EXPERT Evaluation Panel of FWO (Fonds Wetenschappelijk Onderzoek ¿ Vlaanderen).
Evaluator
Fonds Wetenschappelijk Onderzoek Vlaanderen, Belgium Fonds Wetenschappelijk Onderzoek Vlaanderen
2018 - Current EVALUATION OF SEVERO OCHOA Severo Ochoa Centres of Excellence and María de Maeztu Units of Excellence
Evaluator
Severo Ochoa Centres of Excellence and María de Maeztu Units of Excellence
2016 - Current Member of the European Science Foundation (ESF) College of Expert Reviewers
Evaluator
European Science Foundation
2009 - Current Evaluator of research units and research projects by invitation of the German Science Foundation (DFG)
Evaluator
Deutsche Forschungsgemeinschaft
2019/09/02 - 2024/01/20 COORDINATOR of the evaluation process of Scientific Reports from FCT Projects (Experimental Biology and Biochemistry)
Coordinator
Fundação para a Ciência e a Tecnologia, Portugal Fundação para a Ciência e a Tecnologia
Distinctions

Award

1995 Prize - "Oeiras, A Ciência e o Desenvolvimento"
Camara Municipal de Oeiras, Portugal

Fundacao Marques de Pombal, Portugal
1993 "Crystal Award" - Crystallization prize for the first molybdopterin enzyme
Max-Planck-Institut für Biochemie Proteomics und Signaltransduktion, Germany
1978 Hylleraas Prize
Uppsala Universitet Institutionen för biokemi och organisk kemi, Sweden

Other distinction

2019 Member of the Scientific Advisory Committee of the Spanish Synchrotron ALBA (Portuguese Observer )
Fundação para a Ciência e a Tecnologia, Portugal
2019 Member of the Evaluation Panel (BIO1RP) from the Research Foundation from Flanders (FWO)
2019 President of the Scientific Committee of the European Union Science Olympiad (EUSO)
European Union Science Olympiad (EUSO), Ireland
2017 Co-Editor, Acta Crystallographica Section F
International Union of Crystallography, United Kingdom
2016 “100 Mulheres na Ciência” (100 Women in Science )
Pavilion of Knowledge, Portugal
2012 Portuguese Observer to the Council of the European Synchrotron Radiation Facility (ERSF), Grenoble
Fundação para a Ciência e a Tecnologia, Portugal
2010 Executive Committee of the European Synchrotron User Organization (ESUO)
2006 Member of the Editorial Advisory Board of the Journal of Biological Inorganic Chemistry
Society of Biological Inorganic Chemistry, Italy