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Having completed a Bachelors Degree in Biology at the Faculdade de Ciências of the University of Lisbon and a Masters Degree in Microbiology at the Instituto Superior Técnico (IST) of the University of Lisbon, i began my research career as a research assistant working under the Lactosynt project at the Institute for Bioengineering and Biosciences (iBB) of IST, where i continued the work i developed during my Masters Thesis. This mostly consisted in the development of a CRISPR/Cas9 based genome editing plasmid for use in Lactococcus lactis, with the intent of creating an improved strain for plasmid DNA production. Following my time in the Lactosynt project, i carried out research on the behalf of the Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento (IST-ID) for Akron Biotech. This work involved screening several Escherichia coli strains for improved plasmid DNA production, mostly through incubation in several defined media, as well as assembly of shotgun sequencing data into full genomes and their subsequent analysis. Currently, i’m working as a research assistant included in the NanoSens-RNA project taking place at the Centro de Química Estrutural (CQE) and iBB of IST, where i’m designing and developing DNA origami nanostructures for use in novel biosensors, employing plasmonic nanoparticles. My research interests include general molecular biology, with particular focus in strain engineering of bacteria using CRISPR/Cas9 technologies, as well as DNA nanostructure origami and their various applications.
Identification

Personal identification

Full name
José Miguel Miquelão Santos

Citation names

  • Santos, José

Author identifiers

Ciência ID
3510-7BD2-BCD1
ORCID iD
0000-0002-2153-2423

Email addresses

  • josemiquelao@tecnico.ulisboa.pt (Professional)

Knowledge fields

  • Engineering and Technology - Industrial Biotechnology - Bio-derived Novel Materials
  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Microbiology
  • Exact Sciences - Computer and Information Sciences - Bioinformatics
  • Engineering and Technology - Nano-technology

Languages

Language Speaking Reading Writing Listening Peer-review
Portuguese (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2023/07/04 - 2023/07/07
Concluded
15th International Course on Time-resolved Microscopy and Correlation Spectroscopy (Outros)
PicoQuant GmbH, Germany
2023/06/14 - 2023/06/16
Concluded
Introduction to R programming and biological data analysis (Outros)
Universidade de Lisboa Centro de Ecologia Evolução e Alterações Ambientais, Portugal
2023/05/11 - 2023/05/19
Concluded
Training Course on Ethical and Social Aspects in Laboratory Genetics (Outros)
Universidade de Coimbra Faculdade de Medicina, Portugal
Approved
2017/09/05 - 2019/11/15
Concluded
Microbiologia (Mestrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"CRISPR/Cas9-based genome editing of lactic acid bacteria for the improvement of biopharmaceutical-grade plasmid and recombinant protein production" (THESIS/DISSERTATION)
16.98
2012/09/12 - 2016/02/05
Concluded
Biologia (Licenciatura)
Major in Biologia Molecular e Genética
Universidade de Lisboa Faculdade de Ciências, Portugal
12
Affiliation

Science

Category
Host institution
Employer
2023/05/15 - Current Research Assistant (Research) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Universidade de Lisboa Centro de Química Estrutural, Portugal
(...)
2022/05/09 - 2022/11/30 Research Assistant (Research) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2020/01/01 - 2021/09/30 Research Assistant (Research) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Projects

Grant

Designation Funders
2023/03/01 - Current A Gold Nanosensor for Single-Molecule Detection of RNA Biomarkers in a Miniaturized Microscope
Researcher
Universidade de Lisboa Centro de Química Estrutural, Portugal

Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
Outputs

Publications

Conference poster
  1. Santos, José; Monteiro, Gabriel António Amaro; Duarte Miguel de França Teixeira dos Prazeres; Duarte, Sofia Oliveira Dias. "Deletion of the nuclease gene nth leads to a decrease in plasmid DNA yields in Lactococcus lactis subsp. lactis LMG19460". Paper presented in 13th International Symposium on Lactic Acid Bacteria, 2021.
  2. Santos, José; Monteiro, Gabriel António Amaro; Prazeres, Miguel; Duarte, Sofia Oliveira Dias. "Application of a CRISPR/Cas9-based genome editing system for improved biopharmaceutical grade plasmid DNA and recombinant protein yields in Lactococcus lactis". Paper presented in ASBE V - Applied Synthetic Biology in Europe, 2020.
  3. Santos, José; Monteiro, Gabriel António Amaro; Prazeres, Miguel; Duarte, Sofia Oliveira Dias. "Adaptation of a one-plasmid genome editing system for increased production of biopharmaceutical grade recombinant molecules in Lactococcus lactis". Paper presented in Microbiotec19 - Congress of Microbiology and Biotechnology 2019, 2019.
Preprint
  1. Santos, José Miguel Miquelão; Monteiro, Gabriel António Amaro; Prazeres, Duarte Miguel de França Teixeira dos; Duarte, Sofia de Oliveira Dias. "One-plasmid based CRISPR-Cas9 editing for gene deletion in Lactococcus lactis". 2021. http://dx.doi.org/10.21203/rs.3.rs-306012/v1.
    10.21203/rs.3.rs-306012/v1
Thesis / Dissertation
  1. Santos, José. "CRISPR/Cas9-Based Genome Editing Of Lactic Acid Bacteria For The Improvement Of Biopharmaceutical-Grade Plasmid And Recombinant Protein Production". Master, Universidade de Lisboa Instituto Superior Técnico, 2019. https://fenix.tecnico.ulisboa.pt/cursos/microbio/dissertacao/283828618790285.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/04/07 Development of a multiple plasmid system, CRISPR/Cas9-based, for genome editing of Lactococcus lactis subsp. Lactis LMG19460 Ibero-American Congress of Biotechnology 2022 - BioIberoAmerica2022
(Braga, Portugal)
2021/11/26 Targeted removal of the nth nuclease gene from Lactococcus lactis subsp. lactis LMG19460 and its effects on plasmid DNA yield Microbiotec21 - Congress of Microbiology and Biotechnology 2021
Portuguese Society of Microbiology; Portuguese Society of Biotechnology; (Portugal)