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I am a research assistant at the Stem Cell Engineering Research Group (SCERG) of the Institute for Bioengineering and Biosciences (iBB) and the Associate Laboratory — Institute for Health and Bioeconomy (i4HB). My work, carried out as part of a collaboration agreement between iBB and Phoenestra GmbH, an Austrian biotech startup, involves the use of stirred-tank bioreactors for the expansion of human induced pluripotent stem cells (hiPSCs) and the production of extracellular vesicles. Currently, I am also participating as a researcher in one of the ongoing projects at iBB, namely SMART, funded by Fundação para a Ciência e a Tecnologia. Additionally, I am enrolled as a PhD student in Bioengineering at Instituto Superior Técnico of the University of Lisbon. From September 2016 to February 2022, I attended the Integrated Master's in Biomedical Engineering program at NOVA School of Science and Technology (NOVA SST). During this time, I carried out internships at the Microbial Development Lab of the Instituto de Tecnologia Química e Biológica António Xavier (ITQB), where I conceived plasmid vectors and performed bacterial transformation, and the Neuronal Growth and Plasticity Lab of the Centro de Estudos de Doenças Crónicas (CEDOC), where I manipulated and studied Drosophila melanogaster animal models. I undertook my master's thesis at SCERG-iBB, with the objective of exploring and validating cost-efficient strategies for expanding hiPSCs in vertical-wheel bioreactors. For this purpose, I developed a computational tool for the economic modeling of an hiPSC expansion bioprocess and tested B8, a novel pluripotent cell culture medium. Upon graduating, I was distinguished with a NOVA SST Merit Scholarship and received the Best Engineering Student of NOVA SST and Best Biomedical Engineering Student of NOVA SST awards. To date, I have participated as a researcher in 1 concluded project, have published 2 articles in peer-reviewed journals, published 1 book chapter and participated in 11 scientific events, having carried out 7 poster presentations and 1 keynote oral presentation. The computational model I developed is open source and is available on GitHub (https://github.com/wssalvador/BEMSCA).
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Identification

Personal identification

Full name
William Lewis Sullivan Coutinho de Oliveira e Sousa Salvador
Date of birth
1998/06/12
Gender
Male

Citation names

  • Salvador, William O.S.
  • Salvador, William

Author identifiers

Ciência ID
351A-98C4-E403
ORCID iD
0000-0002-5539-3433

Email addresses

  • william.salvador@tecnico.ulisboa.pt (Professional)
  • w.s.salvador@gmail.com (Personal)

Websites

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism
  • Engineering and Technology - Industrial Biotechnology - Bioprocessing Technologies

Languages

Language Speaking Reading Writing Listening Peer-review
English (Mother tongue)
Portuguese (Mother tongue)
Education
Degree Classification
2022/04/05 - 2027/04/05
Ongoing
 Bioengineering (Doutoramento)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"An Integrated Approach to the Production of Extracellular Vesicles from Human Induced Pluripotent Stem Cells in Stirred-Tank Bioreactors" (THESIS/DISSERTATION)
2016/09/19 - 2022/02/05
Concluded
 Biomedical Engineering (Mestrado integrado)
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Towards Cost-Efficient Culture of Human Induced Pluripotent Stem Cells in Vertical-Wheel Bioreactors" (THESIS/DISSERTATION)
 17/20 (1st cycle); 19/20 (2nd cycle); 20/20 (Thesis)
Affiliation

Science

Category
Host institution 		Employer
2022/04/01 - Current Research Assistant (Research) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Projects

Grant

Designation Funders
2024/11/01 - 2027/10/31 An Integrated Process for the Production of Extracellular Vesicles from Human Induced Pluripotent Stem Cells in Stirred-Tank Bioreactors
2024.05042.BD
PhD Student Fellow
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2023/01/13 - 2025/01/31 SMART - New technologies for Stem cell Manufacturing bioprocess monitoring and ARTificial intelligence-based modeling
PTDC/EQU-EQU/3853/2020
Researcher
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2022/11/01 - 2024/11/01 VitaminSea - Enhancing Cost-Effective and Efficient Nutrient Delivery in Fish Embryonic Stem Cell Culture Medium via Functional Bioactive Microcarriers
22-CM-PT-DG-1-317
Researcher
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
 Good Food Institute
Concluded
2022/04/01 - 2024/10/31 Phoenestra (PhD Student Research Grant)
1801P.01165.1.01
PhD Student Fellow
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
 Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento
Concluded
Outputs

Publications

Book chapter
  1. Salvador, William O.S.; Nogueira, Diogo E.S.; Bovolato, Ana L.C.; Ferreira, Frederico; Joaquim M.S. Cabral; Rodrigues, Carlos A.V.. Corresponding author: Rodrigues, Carlos A.V.. "Bringing cellular agriculture to the table: the role of animal cell bioreactors". In Cellular Agriculture: Technology, Society, Sustainability and Science, edited by Fraser, Evan D.G.; Kaplan, David L.; Newman, Lenore; Yada, Rickey Y., 161-175. Elsevier, 2024.
    Published
Conference abstract
  1. Salvador, William O.S.; Nogueira, Diogo E.S.; Ferreira, Frederico; Joaquim M.S. Cabral; Rodrigues, Carlos A.V.. Corresponding author: Rodrigues, Carlos A.V.. "A Novel Decision Support Tool for Optimally Designing Large-Scale Stem Cell Expansion Bioprocesses". Paper presented in RESIM 2022, Leiria, 2022.
    Published
Conference poster
  1. Salvador, William O.S.. "An Integrated Approach to the Production of Extracellular Vesicles from Human Induced Pluripotent Stem Cells in Stirred-Tank Bioreactors". Paper presented in PhD Open Days 2024 - 10th Edition, 2024.
  2. Salvador, William O.S.; Nogueira, Diogo E.S.; Joaquim M.S. Cabral; Klaus Graumann; Rodrigues, Carlos A.V.. Corresponding author: Rodrigues, Carlos A.V.. "Stem Cell Culture in Stirred-Tank Bioreactors: Optimization and Automation of Feeding Strategies for Achieving Economic Viability". Paper presented in 2nd International Conference of the Portuguese Association for Cellular Agriculture, 2024.
  3. Salvador, William O.S.; Nogueira, Diogo E.S.; Ferreira, Frederico; Joaquim M.S. Cabral; Klaus Graumann; Rodrigues, Carlos A.V.. Corresponding author: Rodrigues, Carlos A.V.. "An Integrated Approach to the Production of Extracellular Vesicles from Human Induced Pluripotent Stem Cells in Stirred-Tank Bioreactors". Paper presented in 3rd iBB Workshop, 2023.
  4. Salvador, William O.S.; Nogueira, Diogo E.S.; Ferreira, Frederico; Joaquim M.S. Cabral; Rodrigues, Carlos A.V.. Corresponding author: Rodrigues, Carlos A.V.. "BEMSCA: A Novel Decision Support Tool for the Optimal Design of Large-Scale Stem Cell Expansion Bioprocesses". Paper presented in 1st International Conference of the Portuguese Association for Cellular Agriculture, 2023.
  5. Salvador, William O.S.; Nogueira, Diogo E.S.; Ferreira, Frederico; Joaquim M.S. Cabral; Rodrigues, Carlos A.V.. Corresponding author: Rodrigues, Carlos A.V.. "BEMSCA: A Novel Decision Support Tool for Optimally Designing Large-Scale Stem Cell Expansion Bioprocesses". Paper presented in TERMIS-EU 2023, 2023.
  6. Salvador, William O.S.; Nogueira, Diogo E.S.; Ferreira, Frederico; Joaquim M.S. Cabral; Rodrigues, Carlos A.V.. Corresponding author: Rodrigues, Carlos A.V.. "BEMSCA: A Novel Decision Support Tool for the Optimal Design of a Large-Scale Human Induced Pluripotent Stem Cell Expansion Bioprocess". Paper presented in 2nd iBB Workshop, 2022.
  7. Salvador, William O.S.; Nogueira, Diogo E.S.; Ferreira, Frederico; Joaquim M.S. Cabral; Rodrigues, Carlos A.V.. Corresponding author: Rodrigues, Carlos A.V.. "BEMSCA: A Novel Decision Support Tool for the Optimal Design of a Large-Scale Human Induced Pluripotent Stem Cell Expansion Bioprocess". Paper presented in 27th ESACT Meeting, 2022.
Journal article
  1. Salvador, William O.S.; Nogueira, Diogo E.S.; Ferreira, Frederico; Joaquim M.S. Cabral; Rodrigues, Carlos A.V.. Corresponding author: Rodrigues, Carlos A.V.. "BEMSCA: A novel decision support tool applied to the optimal design of a large-scale human induced pluripotent stem cell expansion bioprocess". Current Research in Biotechnology 6 (2023): http://dx.doi.org/10.1016/j.crbiot.2023.100163.
    Open access • Published • 10.1016/j.crbiot.2023.100163
  2. Salvador, William O.S.; Inês A. B. Ribeiro; Nogueira, Diogo E.S.; Ferreira, Frederico; Joaquim M.S. Cabral; Rodrigues, Carlos A.V.. Corresponding author: Rodrigues, Carlos A.V.. "Bioprocess Economic Modeling: Decision Support Tools for the Development of Stem Cell Therapy Products". Bioengineering 9 12 (2022): https://www.mdpi.com/2306-5354/9/12/791.
    Open access • Published • 10.3390/bioengineering9120791
Thesis / Dissertation
  1. "Towards Cost-Efficient Culture of Human Induced Pluripotent Stem Cells in Vertical-Wheel Bioreactors". Master, Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, 2022. http://hdl.handle.net/10362/138625.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022/06/03 A Novel Decision Support Tool for Optimally Designing Large-Scale Stem Cell Expansion Bioprocesses RESIM 2022
Politécnico de Leiria Centro para o Desenvolvimento Rápido e Sustentado de Produto (Leiria, Portugal)

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2024/11/04 - 2024/11/05 Poster presentation at the PhD Open Days 2024 - 10th Edition and participation in the activities of its scientific program.
Exhibition
 PhD Open Days 2024 - 10th Edition
Universidade de Lisboa Instituto Superior Técnico, Portugal
2024/10/04 - 2024/10/05 Poster presentation at the 2nd International Conference of the Portuguese Association for Cellular Agriculture and participation in the activities of its scientific program.
Conference
 2nd International Conference of the Portuguese Association for Cellular Agriculture
CellAgri Portugal, Portugal
2023/11/13 - 2023/11/13 Poster presentation at the 3rd iBB Workshop and participation in the activities of its scientific program.
Conference
 3rd iBB Workshop
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2023/09/07 - 2023/09/08 Poster presentation at the 1st International Conference of the Portuguese Association for Cellular Agriculture and participation in the activities of its scientific program.
Conference
 1st International Conference of the Portuguese Association for Cellular Agriculture
CellAgri Portugal, Portugal
2023/06/22 - 2023/06/23 Participation in the 2nd PNEV Meeting.
Conference
 2nd PNEV Meeting
Portuguese Network on Extracellular Vesicles, Portugal
2023/03/28 - 2023/03/31 Poster presentation at the TERMIS-EU 2023 conference and participation in the activities of its scientific program.
Conference
 TERMIS-EU 2023
Tissue Engineering and Regenerative Medicine International Society, United States
2022/12/19 - 2022/12/19 Participation in the Associate Laboratory i4HB Kick-Off Meeting.
Meeting
 i4HB Kick-Off Meeting
Institute for Health and Bioeconomy, Portugal
2022/10/28 - 2022/10/29 Participation in Small New World, a meeting organized jointly by the Austrian and German Societies for Extracellular Vesicles (ASEV and GSEV, respectively).
Conference
 Small New World
Austrian Society for Extracellular Vesicles, Austria

German Society for Extracellular Vesicles, Germany
2022/09/23 - 2022/09/23 Poster presentation at the 2nd iBB Workshop and participation in the activities of its scientific program.
Conference
 2nd iBB Workshop
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2022/06/26 - 2022/06/29 Poster presentation at the 27th ESACT Meeting and participation in the activities of its scientific program.
Conference
 27th ESACT Meeting
European Society for Animal Cell Technology, Germany
2021/09/03 - 2021/09/05 Participation in the Biomedical Engineering Summit, an event organized by the Associação Nacional de Estudantes de Engenharia Biomédica (ANEEB).
Conference
 Biomedical Engineering Summit 2021
Associação Nacional de Estudantes de Engenharia Biomédica, Portugal
2016/05/30 - 2016/06/01 See description of the VIII Mostra de Ciência below.
Exhibition
 X Mostra Nacional de Ciência
Fundação da Juventude, Portugal

Fundação EDP, Portugal
2015/05/28 - 2015/05/30 See description of the VIII Mostra de Ciência below.
Exhibition
 IX Mostra Nacional de Ciência
Fundação da Juventude, Portugal

Fundação EDP, Portugal
2014/05/29 - 2014/05/31 Poster presentation at the VIII Mostra Nacional de Ciência, an annual event organized by Fundação da Juventude and Fundação EDP which recognizes and rewards promising young scientists and researchers at the national level.
Exhibition
 VIII Mostra Nacional de Ciência
Fundação da Juventude, Portugal

Fundação EDP, Portugal
Distinctions

Award

2022 Best Engineering Student 2020/21
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022 Best Biomedical Engineering Student 2020/21
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022 Merit Scholarship 2020/21
Universidade NOVA de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Direção-Geral do Ensino Superior, Portugal