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Dr Bernardes graduated in Biological Engineering (MSc) in 2008 at Instituto Superior Técnico (IST) (16/20), during which he visited the Molecular Cell Biology Lab at KULeuven (Belgium) for his final MSc Dissertation (20/20). In 2013, he obtained his PhD in Biotechnology at IST (Pass with Distinction), in collaboration with IPATIMUP, Porto. His PhD studies focused on the use of azurin as an anticancer protein for aggressive breast cancer which overexpressed P-cadherin. Since 2014 until November 2018, as a post-doc in the Institute for Bioengineering and Biosciences (iBB) and IPATIMUP/i3S he expanded his studies to the effects of azurin at lipid raft microdomains which function as platforms for protein receptors to influence intra-cellular signaling and the response to anti-cancer drugs. In 2018, Dr Bernardes signed a research contract as Researcher at iBB-IST to focus on developing (i) protein- and peptide-based strategies to enhance targeted delivery of nanosized drug delivery systems, and (ii) engineered cell-based therapies for cancer with clinically relevant therapeutic cells. In 2019, he joined the Department of Bioengineering at IST as Invited Assistant Professor, teaching Genetic Engineering, Molecular Biotechnology, and Biochemistry & Molecular Biology of the Cell to 1st and 2nd degrees. He has (co-)authored 28 records (in Web of Science, H-index 13) (in the last 5 years total of 16, 3 as co-corresponding author, 4 book chapters and 1 national patent granted). In the last 5 years, he (co-)supervised 8 MSC thesis and 2 PhD thesis (one as Main Supervisor). He was member of the team of 5 FCT-funded projects with a total funding > 1M¿. As PI, he gathered 35.5 k¿ to study new approaches to develop new therapies for cancer. In 2016, he was awarded a Travel Grant from Boehringer Ingelheim Fonds to attend the ¿Genome Engineering: CRIPRS/Cas9 course at EMBL. He was awarded the EACR Best Poster ASPIC 2018. He has reviewed for national funding agencies (ANI) and journals (Molecular Therapy, Cancer Nanotechnology, Cell Cycle, IJMS, Cells, Cancers), and was external examiner for 26 MSc and 2 PhD theses. He is intensively involved in communication of science to lay audiences, namely he regularly gives talks for high school students in Open Labs from DBE@IST, and chaired session in events organized by the student community, namely the Bioengineering week at IST and the session dedicated to the challenges and ethical limits of genetic manipulation in the conference Engineering to the Limits (May 2021) organized by the Students Association of IST. He was also member of the Organization Committee of the 1st iBB Workshop - Fostering collaborative research (Oct 29th 2019). During the COVID19 pandemic, Dr Bernardes joined the iBB Taskforce which implemented and certified the iBB-CTN Covid testing Lab, assisting in the efforts to provide COVID tests (in the times they were scarce) mainly to elderly facilities in the Lisbon Metropolitan Area. He provided scientific supervision and coordinated the volunteers¿ teams who performed the tests. In 2021, he visited the Rhoades Lab, at the School of Arts and Sciences of the University of Pennsylvania as a Fulbright Visiting Researcher (Scholarship awarded by the Portugal Fulbright Committee), where he received training in proximity labeling and advanced fluorescence microscopy techniques and data analysis applied to protein and peptide delivery techniques.
Identification

Personal identification

Full name
Nuno Bernardes

Citation names

  • Bernardes, N
  • Bernardes, Nuno

Author identifiers

Ciência ID
3610-B70F-1BDA
ORCID iD
0000-0002-4495-6608
Google Scholar ID
https://scholar.google.com/citations?hl=en&user=MzlGL1IAAAAJ
Researcher Id
F-7826-2014

Addresses

  • IBB/IST-Institute for Bioengineering and Biosciences, Instituto Superior Técnico. Avenida Rovisco Pais, 1, 1049-001, Lisboa, Lisboa, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Cell Biology
  • Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism
  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Intermediate (B1) Advanced (C1)
Portuguese (Mother tongue)
Education
Degree Classification
2013/10
Concluded
Biotecnologia (Doutoramento)
Major in Sem especialidade
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Bacterial protein azurin as a new therapeutic tool to treat poor prognosis breast cancer with overexpression of P-cadherin " (THESIS/DISSERTATION)
Pass with Distinction
2008
Concluded
Engenharia Biológica (Mestrado integrado)
Universidade de Lisboa Instituto Superior Técnico, Portugal
"Involvment of cAMP pathway in apoptosis in a glucose-negative mutant" (THESIS/DISSERTATION)
16/20
Affiliation

Science

Category
Host institution
Employer
2024/05/01 - Current Auxiliary Researcher (Research) Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2018/11/15 - 2024/04/30 Contracted Researcher (Research) Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
2021/09/01 - 2021/12/31 Visiting Researcher (Research) University of Pennsylvania School of Arts and Sciences, United States
University of Pennsylvania School of Arts and Sciences, United States
2014/04/01 - 2018/11/14 Postdoc (Research) Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Teaching in Higher Education

Category
Host institution
Employer
2019/09/01 - Current Invited Assistant Professor (University Teacher) Universidade de Lisboa Instituto Superior Técnico, Portugal
2012/01/01 - 2012/07/01 Invited Adjunct Teacher (Polytechnic Teacher) Instituto Politécnico de Setúbal Escola Superior de Tecnologia do Barreiro, Portugal

Others

Category
Host institution
Employer
2009/01/01 - 2013/10/17 PhD student in Biotechnology Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
(...)
2008/02/01 - 2008/08/31 Research training Katholieke Universiteit Leuven Departement Cellulaire en Moleculaire Geneeskunde, Belgium
Projects

Grant

Designation Funders
2021/02/01 - Current CAPTURA _ IST-COVID Lab
72536
Researcher
Universidade de Lisboa Instituto Superior Técnico, Portugal
Autoridade de Gestão do Programa Operacional Competitividade e Internacionalização
Ongoing
2018/09/01 - 2021/12/31 Cell penetrating p28 peptide-mediated delivery of nanomedicines for cancer treatment
PTDC/BTM-SAL/30034/2017
Researcher
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/09/01 - 2021/06/30 Engineering of Smart Exosomes for Amyloid-beta Clearance in Alzheimer
PTDC/BTM-SAL/31057/2017
Researcher
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/09/01 - 2021/06/30 The aggregation-prone structures of Huntingtin exon 1 at the single-molecule level: Influence of membranes and implications in Huntington’s disease
PTDC/BIA-BFS/30959/2017
Researcher
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2010/01/01 - 2012/06/30 Bacterial protein azurin as a new candidate drug to treat poor-prognosis breast cancer
PTDC/EBB-BIO/100326/2008
PhD Student Fellow
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Fundação para a Ciência e a Tecnologia
Concluded

Other

Designation Funders
2021/01/01 - 2022/06/30 ESCAPE - Mapping the uptake and intracellular processing of p28 and p28-decorated extracellular vesicles to boost the functional transfer of therapeutic RNAs
iBB/2022
Principal investigator
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Ongoing
2015/07/01 - 2016/12/31 Azurin-expressing Mesenchymal Stem / Stromal Cells in anticancer therapies
iBB/2015/12
Principal investigator
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal
Concluded
Outputs

Publications

Book chapter
  1. "Overcoming the Plasma Membrane". In Organelle and Molecular Targeting, edited by Lara Scheherazade Milane; Mansoor M. Amiji. United States: Taylor and Franscis Group, 2021.
    Published
  2. Garizo, Ana Rita; Bernardes, Nuno; Chakrabarty, Ananda M.; Fialho, Arsénio M.. "The Anticancer Potential of the Bacterial Protein Azurin and Its Derived Peptide p28". In Microbial Infections and Cancer Therapy, edited by Professor Doutor Arsénio do Carmo Sales Mendes Fialho; Ananda Chakrabarty, 319-338. Jenny Stanford Publishing, 2019.
    Accepted • 10.1201/9781351041904-9
  3. Coelho, Lígia F.; Bernardes, Nuno; Fialho, Arsénio M.. "Prospective Therapeutic Applications of Bacteriocins as Anticancer Agents". In Microbial Infections and Cancer Therapy, edited by Professor Doutor Arsénio do Carmo Sales Mendes Fialho; Ananda Chakrabarty, 339-366. Jenny Stanford Publishing, 2019.
    10.1201/9781351041904-10
  4. Bernardes, Nuno. "Gene Delivery as a Tool to Improve Therapeutic Features of Mesenchymal Stromal Cells: Methods and Applications". In Gene Delivery: Methods and Applications, edited by Vanessa Zimmer. NOVA SCIENCE PUBLISHERS, INC., 2019.
Conference abstract
  1. Sousa, T; Bernardes, N; Coutinho, A; Prieto, M; Melo, A.M. Corresponding author: Melo, A.M. "The conformational dynamics of the flanking polyQ regions in the membrane-bound state of huntingtin exon 1". Paper presented in Biophysical Society 66th Annual Meeting, San Franscisco, 2022.
    Published • 10.1016/j.bpj.2021.11.1250
  2. Fuzeta MA; Bernardes, N; Roefs MT; van de Wakker, S. I; Olijve, W; Lin, Y; Jung, S.; et al. Corresponding author: da Silva, C. L. "Scalable bioreactor production and angiogenic potential of extracellular vesicles derived from human mesenchymal stromal cells". Paper presented in 28th Annual Meeting of the International Socienty for Cell Therapies, 2022.
    Published
  3. Sousa, Tânia; Bernardes, Nuno; Coutinho, Ana; Prieto, Manuel; Melo, Ana M.. "Effects of the Flanking polyQ Regions and Membrane Physical Properties on Huntingtin Binding to Lipid Vesicles". Paper presented in 65th Biophysical society annual meeting, 2021.
    Published • 10.1016/j.bpj.2020.11.446
  4. Fuzeta, M.A.; Oliveira, F.D; Costa, A.C.; Fernandes-Platzgummer, A.; Jung, S.; Tseng, R.; Milligan, W.; et al. "Scalable Production of Human Mesenchymal Stromal Cell (MSC)-Derived Extracellular Vesicles in Microcarrier-based Bioreactors under Xeno(geneic)-free Conditions". Paper presented in ISCT Paris Virtual 2020, 2020.
    10.1016/j.jcyt.2020.03.499
  5. Bernardes, N; Ribeiro, A. S; Mota, B; Estevens, T; Matos, R. G; (Arraiano, C. M.; Seruca, R; Paredes, J.; Fialho, A. M. Corresponding author: Fialho, A. M. "Bacterial Protein Azurin as a New Candidate Drug to Treat P-cadherin Overexpressing Breast Cancer". Paper presented in 22nd Biennial Congress of the European-Association-for-Cancer-Research, 2012.
    Published
Conference poster
  1. Santos G; Bernardes, N; Braz BS; Fialho AM; Gonçalves L; Delgado E. "Ex Vivo Permeation Of P28, a Cell-Penetrating Peptide To Enhance The Ocular Delivery Of Erythropoietin Encapsulated Into Nanoparticles". Paper presented in Montenegro International Veterinary Congress XIX, 2023.
  2. Bernardes, N; da Silva CL; Fialho AM. Corresponding author: Bernardes, N. "Tumor-targeting strategies for the development of selective therapies". Paper presented in 2023 USA-Portugal Leaders in Cancer Research, 2023.
  3. Gonçalves PP; de ALmeida Fuzeta M; da Silva CL; Bernardes, Nuno. Corresponding author: Bernardes, Nuno. "Surface modification of mesenchymal stromal cell derived extracellular vesicles with peptides for targeted cancer therapy". Paper presented in 2nd meeting of the PORTUGUESE NETWORK ON EXTRACELLULAR VESICLES, 2023.
  4. Tiago P Dias; Joana Machado; Bernardes, Nuno; Fábio Fernandes. "Bioengineered extracellular vesicles with neprilysin activity". Paper presented in 2nd meeting of the PORTUGUESE NETWORK ON EXTRACELLULAR VESICLES, 2023.
  5. Diogo Gonçalves; Ricardo Silva; Bernardes, Nuno; Ana M. Azevedo; Sandra N. Pinto; Fábio Fernandes. "Analysis of the cytoprotective potential of extracellular vesicles against bacterial pore forming toxins". Paper presented in SNEV 2023 Virtual Conference, 2023.
  6. Sousa, T; Damas, G; Bernardes, N; Coutinho, A; Prieto, M; Melo, A.M.. Corresponding author: Melo, A.M.. "The flanking polyQ regions of Huntingtin exon 1 display distinct dynamic signatures in its membrane-bound state: insights into Huntington´s disease". Paper presented in 25th IUBMB Congress, the 46th FEBS Congress and the 15th PABMB Congress, 2022.
  7. Sousa, T; Damas, G; Bernardes, N; Coutinho, A; Prieto, M; Melo, A.M. Corresponding author: Melo, A.M. "The flanking polyQ regions of Huntingtin exon 1 display distinct conformational signatures in its membrane¬bound state: insights into Huntington´s disease". Paper presented in Gordon Research Conference (GRC) in Intrinsically Disordered Proteins, 2022.
  8. Sousa, T; Bernardes, N; Coutinho, A; Prieto, M; Melo, A.M. Corresponding author: Melo, A.M. "The conformational dynamics of the flanking polyQ regions in the membrane-bound state of huntingtin exon 1". Paper presented in 20th International Union for Pure and Applied Biophysics (IUPAB), 2021.
    10.1016/j.bpj.2021.11.1250
  9. Sousa, T; Bernardes, N; Coutinho, A; Prieto, M; Melo, A.M. Corresponding author: Melo, A.M. "Effects of the flanking polyQ regions and membrane physical properties on huntingtin binding to lipid vesicles". Paper presented in Biophysical Society 65th Annual Meeting, 2021.
    10.1016/j.bpj.2020.11.446
  10. Miguel de Almeida Fuzeta; Bernardes, N; van de Wakker, Simonides I; Roefs, Marieke T; Olijve, Wilte; Fernandes-Platzgummer, Ana; Vader, Pieter; et al. Corresponding author: da Silva, CL. "Scalable bioreactor production and angiogenic potential of extracellular vesicles derived from human mesenchymal stromal cells.". Paper presented in International Society for Extracellular Vesicles Meeting 2021, 2021.
  11. Fuzeta, MA; Oliveira, Filipa D; Costa, Ana Catarina; Fernandes-Platzgummer, Ana; Bernardes, N; Gaspar, Diana; Cabral, Joaquim M. S; Lobato da Silva, Cláudia. Corresponding author: Lobato da Silva, Cláudia. "Scalable Production of Human Mesenchymal Stem/Stromal Cell (MSC)-Derived Extracellular Vesicles in Vertical-Wheel Bioreactors for Cancer Therapy". Paper presented in Stem Cell Community Day. Lisboa, Portugal, 2019.
  12. Fuzeta, Miguel de Almeida ; Oliveira, Filipa D; Fernandes-Platzgummer, Ana; Bernardes, N; Gaspar, Diana; Cabral, Joaquim M. S; Lobato da Silva, Cláudia. Corresponding author: Lobato da Silva, Cláudia. "Manufacturing Extracellular Vesicles Derived from Human Mesenchymal Stem/Stromal Cells (MSC) in Vertical-Wheel Bioreactors". Paper presented in Encontro Ciência 2019, 2019.
  13. Bernardes, Nuno. "Modulation of membrane order and interaction with lipid rafts by azurin increases sensitivity to anti-cancer drugs". Paper presented in 3rd ASPIC International Congress, 2018.
  14. Silva, Marília; Almeida, Andreia; Beckman, Evguenia; Cabral, Joaquim; Fialho, Arsénio; Bernardes, Nuno; da Silva, Cláudia; Bernardes, N. Corresponding author: da Silva, Cláudia. "Development of an anti-cancer cell therapy with genetically-modified mesenchymal stem/stromal cells expressing azurin". Paper presented in International conference of ASPIC- Associação Portuguesa de Investigação em Cancro, 2018.
  15. Bernardes, N. "Azurin, a therapeutic protein that interferes with oncogenic signaling and blocks tumor progression". Paper presented in EMBO|EMBL Symposium: Tumor Microenvironment and signaling, 2014.
  16. Bernardes, N. "Azurin impairs P-cadherin-dependent invasion of breast cancer cells via decreased FAk/Src signaling". Paper presented in EACR22- from Basic Research to Personalized Cancer Treatment, 2012.
  17. Bernardes, N. "Bacterial protein azurin as a new candidate drug to treat p-cadherin-overexpressing breast cancer cells". Paper presented in Molecular Mechanisms in Signal Transduction and Cancer,, 2011.
Journal article
  1. Miguel de Almeida Fuzeta; Pedro P. Gonçalves; Ana Fernandes-Platzgummer; Joaquim M. S. Cabral; Nuno Bernardes; Cláudia L. da Silva. "From Promise to Reality: Bioengineering Strategies to Enhance the Therapeutic Potential of Extracellular Vesicles". Bioengineering (2022): https://doi.org/10.3390/bioengineering9110675.
    10.3390/bioengineering9110675
  2. Luís Borges-Araújo; Marina E. Monteiro; Dalila Mil-Homens; Nuno Bernardes; Maria J. Sarmento; Ana Coutinho; Manuel Prieto; Fábio Fernandes. "Impact of Ca2+-Induced PI(4,5)P2 Clusters on PH-YFP Organization and Protein-Protein Interactions". Biomolecules (2022): https://doi.org/10.3390/biom12070912.
    10.3390/biom12070912
  3. Maria J. Sarmento; Luís Borges-Araújo; Sandra N. Pinto; Nuno Bernardes; Joana C. Ricardo; Ana Coutinho; Manuel Prieto; Fábio Fernandes. "Quantitative FRET Microscopy Reveals a Crucial Role of Cytoskeleton in Promoting PI(4,5)P2 Confinement". International Journal of Molecular Sciences (2021): https://doi.org/10.3390/ijms222111727.
    10.3390/ijms222111727
  4. Ana Rita Garizo; Lígia F. Coelho; Sandra Pinto; Tiago P. Dias; Fábio Fernandes; Nuno Bernardes; Arsénio M. Fialho. "The Azurin-Derived Peptide CT-p19LC Exhibits Membrane-Active Properties and Induces Cancer Cell Death". Biomedicines (2021): https://doi.org/10.3390/biomedicines9091194.
    10.3390/biomedicines9091194
  5. Garizo, Ana Rita; Castro, Flávia; Martins, Cláudia; Almeida, Andreia; Dias, Tiago P.; Fernardes, Fábio; Barrias, Cristina C.; et al. "p28-functionalized PLGA nanoparticles loaded with gefitinib reduce tumor burden and metastases formation on lung cancer". Journal of Controlled Release (2021): http://dx.doi.org/10.1016/j.jconrel.2021.07.035.
    10.1016/j.jconrel.2021.07.035
  6. Pimenta, Andreia I.; Kilcoyne, Michelle; Bernardes, Nuno; Mil-Homens, Dalila; Joshi, Lokesh; Fialho, Arsenio M.. "Burkholderia cenocepacia BCAM2418 -induced antibody inhibits bacterial adhesion, confers protection to infection and enables identification of host glycans as adhesin targets". Cellular Microbiology (2021): http://dx.doi.org/10.1111/cmi.13340.
    10.1111/cmi.13340
  7. Pimenta, Andreia I.; Bernardes, Nuno; Alves, Marta M.; Mil-Homens, Dalila; Fialho, Arsenio M.. "Burkholderia cenocepacia transcriptome during the early contacts with giant plasma membrane vesicles derived from live bronchial epithelial cells". Scientific Reports 11 1 (2021): http://dx.doi.org/10.1038/s41598-021-85222-5.
    10.1038/s41598-021-85222-5
  8. de Almeida Fuzeta, Miguel; Bernardes, Nuno; Oliveira, Filipa D.; Costa, Ana Catarina; Fernandes-Platzgummer, Ana; Farinha, José Paulo; Rodrigues, Carlos A. V.; et al. "Scalable Production of Human Mesenchymal Stromal Cell-Derived Extracellular Vesicles Under Serum-/Xeno-Free Conditions in a Microcarrier-Based Bioreactor Culture System". Frontiers in Cell and Developmental Biology 8 (2020): http://dx.doi.org/10.3389/fcell.2020.553444.
    10.3389/fcell.2020.553444
  9. Silva, Marília; Monteiro, Gabriel Amaro; Fialho, Arsenio M.; Bernardes, Nuno; da Silva, Cláudia Lobato. "Conditioned Medium From Azurin-Expressing Human Mesenchymal Stromal Cells Demonstrates Antitumor Activity Against Breast and Lung Cancer Cell Lines". Frontiers in Cell and Developmental Biology 8 (2020): http://dx.doi.org/10.3389/fcell.2020.00471.
    Open access • 10.3389/fcell.2020.00471
  10. Nuno Bernardes; Ana Rita Garizo; Sandra N. Pinto; Bernardo Caniço; Catarina Perdigão; Fábio Fernandes; Arsenio M. Fialho. "Azurin interaction with the lipid raft components ganglioside GM-1 and caveolin-1 increases membrane fluidity and sensitivity to anti-cancer drugs". Cell Cycle 17 13 (2018): 1649-1666. https://doi.org/10.1080/15384101.2018.1489178.
    10.1080/15384101.2018.1489178
  11. Nuno Bernardes; Arsenio M. Fialho. "Perturbing the Dynamics and Organization of Cell Membrane Components: A New Paradigm for Cancer-Targeted Therapies". International Journal of Molecular Sciences 19 12 (2018): 3871-3871. https://doi.org/10.3390%2Fijms19123871.
    10.3390/ijms19123871
  12. Ken Peeters; Frederik Van Leemputte; Baptiste Fischer; Beatriz M. Bonini; Hector Quezada; Maksym Tsytlonok; Dorien Haesen; et al. "Fructose-1,6-bisphosphate couples glycolytic flux to activation of Ras". Nature Communications 8 1 (2017): https://doi.org/10.1038/s41467-017-01019-z.
    10.1038/s41467-017-01019-z
  13. Bernardes, Nuno; Abreu, Sofia; Carvalho, Filomena A.; Fernandes, Fábio; Santos, Nuno C.; Fialho, Arsénio M.; Bernardes, N. "Modulation of membrane properties of lung cancer cells by azurin enhances the sensitivity to EGFR-targeted therapy and decreased ß1 integrin-mediated adhesion". Cell Cycle (2016): 00-00.
    10.1080/15384101.2016.1172147
  14. M. Fialho, Arsenio; Bernardes, Nuno; M Chakrabarty, Ananda. "Exploring the anticancer potential of the bacterial protein azurin". AIMS Microbiology 2 3 (2016): 292-303. http://dx.doi.org/10.3934/microbiol.2016.3.292.
    Published • 10.3934/microbiol.2016.3.292
  15. Bernardes, Nuno. "Bacterial proteins and peptides in cancer therapy: today and tomorrow.". Bioengineered (2014): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000341290500015&KeyUID=WOS:000341290500015.
    10.4161/bioe.29266
  16. Bernardes, Nuno. "High-throughput molecular profiling of a P-cadherin overexpressing breast cancer model reveals new targets for the anti-cancer bacterial protein azurin.". The International Journal of Biochemistry and Cell Biology (2014): http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000335611500001&KeyUID=WOS:000335611500001.
    10.1016/j.biocel.2014.01.023
  17. Bernardes, N.; Ribeiro, A.S.; Abreu, S.; Mota, B.; Matos, R.G.; Arraiano, C.M.; Seruca, R.; Paredes, J.; Fialho, A.M.. "The Bacterial Protein Azurin Impairs Invasion and FAK/Src Signaling in P-Cadherin-Overexpressing Breast Cancer Cell Models". PLoS ONE 8 7 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84880423678&partnerID=MN8TOARS.
    10.1371/journal.pone.0069023
  18. Bernardes, N.; Chakrabarty, A.M.; Fialho, A.M.. "Engineering of bacterial strains and their products for cancer therapy". Applied Microbiology and Biotechnology 97 12 (2013): 5189-5199. http://www.scopus.com/inward/record.url?eid=2-s2.0-84878687925&partnerID=MN8TOARS.
    10.1007/s00253-013-4926-6
  19. Mil-Homens, D.; Bernardes, N.; Fialho, A.M.. "The antibacterial properties of docosahexaenoic omega-3 fatty acid against the cystic fibrosis multiresistant pathogen Burkholderia cenocepacia". FEMS Microbiology Letters 328 1 (2012): 61-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-84856461868&partnerID=MN8TOARS.
    10.1111/j.1574-6968.2011.02476.x
  20. Fialho, A.M.; Bernardes, N.; Chakrabarty, A.M.. "Recent patents on live bacteria and their products as potential anticancer agents". Recent Patents on Anti-Cancer Drug Discovery 7 1 (2012): 31-55. http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000303310500004&KeyUID=WOS:000303310500004.
  21. Bernardes, N.; Ribeiro, A.S.; Seruca, R.; Paredes, J.; Fialho, A.M.. "Bacterial protein azurin as a new candidate drug to treat untreatable breast cancers". 1st Portuguese Meeting in Biomedical Engineering, ENBENG 2011 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-80053958083&partnerID=MN8TOARS.
    10.1109/ENBENG.2011.6026047
  22. Bernardes, N.; Seruca, R.; Chakrabarty, A.M.; Fialho, A.M.. "Microbial-based therapy of Cancer Current progress and future prospects". Bioengineered Bugs 1 3 (2010): 178-190. http://www.scopus.com/inward/record.url?eid=2-s2.0-79851473784&partnerID=MN8TOARS.
    10.4161/bbug.1.3.10903
Thesis / Dissertation
  1. "Bacterial protein azurin as a new therapeutic tool to treat poor prognosis breast cancer with overexpression of P-cadherin Palavras chave". PhD, Universidade de Lisboa Instituto Superior Técnico, 2013.
  2. "Involvement of the cAMP pathway in apoptosis in a Saccharomyces cerevisiae glucose-negative mutant". Master, Universidade de Lisboa Instituto Superior Técnico, 2008.

Intellectual property

Provisional application for patent
  1. 2023. "POLÍMEROS DE NÚCLEO-CAMADA CATIÓNICOS".
    Disclosed
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2022 Targeting tumors with peptides for the development of new therapeutics iBB Seminars
Institute for Bioengeenering and Biosciences (Oeiras, Portugal)
2022 Tumor-targeting strategies for the development of selective therapies Conferência anual da Rede Saúde da Universidade de Lisboa
Universidade de Lisboa (Lisboa, Portugal)
2019/05/15 Exploring plasma membrane dynamics and organization as targets of the anticancer activity of bacterial azurin Anual Meeting of the Brazilian Society for Biochemistry and Molecular Biology
Brazilian Society for Biochemistry and Molecular Biology (São Paulo, Brazil)
2018/10/12 Azurin increases membrane fluidity and sensitivity to anti-cancer drugs by interacting with lipid raft components GM-1 and caveolin-1 in cancer cells Lisbon Biophysics Networking Meeting: From Protein Dynamics to Membrane Biophysics
Universidade de Lisboa, Instituto Superior Técnico (Lisboa, Portugal)
2011/12 Bacterial protein azurin as a new candidate drug to treat P-cadherin-overexpressing breast cancer MicroBiotec'11
Portuguese societies for microbiologu and biotechnology (Braga, Portugal)
2010 The Anti-HIV properties of bacterial protein azurin Rational Design of HIV Vaccines and Microbicides,
(Lisboa, Portugal)

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2024/03/01 - Current 3D tumor spheroid models to assess the biocompatibility and anticancer properties of p28-functionalized polymeric nanoparticles
Supervisor
Engenharia Química (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2024/02/01 - Current Scalable Production of Extracellular Vesicles Derived from Mesenchymal Stromal Cell Aggregates for Cancer Therapy: In Vitro Screening in 3D Heterotypic Tumor Spheroids
Supervisor
Biotecnologia e Biociências (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2023/09/10 - Current Genetic engineering of Mesenchymal Stromal Cells to express anti-cancer proteins: impact in cell proliferation and profile of the tumor microenvironment using 3D multicellular tumor spheroids
Supervisor
Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2023/09/10 - Current Mapping membrane protein interactors of the anti-cancer cell penetrating peptide p28 by live cell proximity labelling
Supervisor
Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2023/02/01 - 2023/10/31 Tumor spheroids as 3D models to evaluate the efficacy of azurin-expressing Mesenchymal Stromal Cells-secretome as a potential anticancer therapy
Supervisor
Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/10/01 - 2022/10/31 Peptide-functionalized Polymeric Nanoparticles for Cancer Therapy Applications
Supervisor
Microbiologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2022/03/01 - 2022/10/15 Surface modification of mesenchymal stromal cell-derived extracellular vesicles with peptides for targeted cancer therapy
Supervisor of Pedro Espírito Santo Prates Gonçalves
Engenharia Biomédica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/02/01 - 2021/10/21 Genetic engineering of mesenchymal stromal cells to express the anti-cancer protein azurin
Supervisor of Marilia Maria Rodrigues da Silva
Bioengenharia (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/01/01 - 2021/10/20 Scalable Production of Extracellular Vesicles Derived from Mesenchymal Stromal Cells for Cancer-Targeted Drug Delivery
Co-supervisor of MIguel de Almeida Fuzeta
Bioengenharia (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/09/01 - 2019/10/31 Assessment of anticancer activities in marine sponge- and coral- associated bacteria
Co-supervisor
Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/09/01 - 2019/10/31 Ribonucleoprotein (RNP) transfection efficiency of Cas9 endonuclease from Streptococcus pyogenes in human Mesenchymal Stem/Stromal Cells: impact of donor-to-donor variability and different tissue sources
Supervisor
Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/09/01 - 2019/10/31 Expression and purification of recombinant Huntingtin exon 1 using solubilizing fusion tags
Co-supervisor
Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/09/01 - 2018/10/31 Interaction of mesenchymal stem/stromal cell-derived exosomes with human cell lines for different biomedical applications
Supervisor
Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/09/01 - 2018/09/30 Expression and purification of the Streptococcus pyogenes Cas9 protein for ribonucleoprotein transfection strategy and CRISPR/Cas9-based in AAVS1 safe harbor locus
Supervisor
Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/02/01 - 2017/10/31 Genome editing of Mesenchymal Stem/Stromal Cells (MSCs) by CRISPR/Cas9 technology for azurin-based anticancer therapies
Supervisor
Engenharia Biomédica (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/02/01 - 2016/09/30 Interactions between cancer cell populations in vivo: cooperation vs competition
Co-supervisor
Engenharia Biológica
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015/09/01 - 2016/09/30 Azurin-expressing Bone Marrow-Mesenchymal Stem/Stromal Cells in anticancer therapies
Supervisor
Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015/09/01 - 2016/06/30 Anticancer Potencial of Azurin Interaction with lipid rafts: deciphering the role of caveoale
Supervisor
Biologia Humana e Ambiente (Master)
Universidade de Lisboa Faculdade de Ciências, Portugal
2014/09/01 - 2015/09/30 A site-specific mutation (F114A) in the hydrophobic core of the bacterial protein azurin significantly impairs its anticancer activity
Co-supervisor
Microbiologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2013/09/01 - 2014/10/06 Azurin interferes with lipid raft organization by decreasing the membrane protein caveolin-1
Co-supervisor
Biotecnologia (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal

Event organisation

Event name
Type of event (Role)
Institution / Organization
2019/10/29 - 2019/10/29 1st Workshop of the Institute for Bioengineering and Bioscience - Fostering collaborative research (2019/10/29 - 2019/10/29)
Workshop (Member of the Organising Committee)
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Event participation

Activity description
Type of event
Event name
Institution / Organization
2023/07/20 - 2023/07/20 2023 USA-Portugal Leaders in Cancer Research
Meeting
2023 USA-Portugal Leaders in Cancer Research
Fundação Champalimaud, Portugal
2020/02 - 2020/02 Biotecnologia e Inovação terapêutica: células e produtos derivados como agentes anticancerígenos
Seminar
Laboratórios Abertos do Departamento de Bioengenharia
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019/02 - 2019/02 Biotecnologia e Inovação terapêutica: células e produtos derivados como agentes anticancerígenos
Seminar
Laboratórios Abertos do Departamento de Bioengenharia
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018/05/04 - 2018/05/05 Modulation of membrane properties and interaction with lipid rafts components GM-1 and caveolin-1 in cancer cells by azurin increases membrane fluidity and sensitivity to anti-cancer drugs.
Congress
3rd ASPIC Cingress
2018/02 - 2018/02 Biotecnologia e Inovação terapêutica: células e produtos derivados como agentes anticacerígenos
Seminar
Laboratórios Abertos do Departamento de Bioengeharia
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/02/10 - 2017/02/10 Biotecnologia e Inovação terapêutica: bactérias e produtos seus derivados como agentes anticancerígenos
Seminar
Laboratórios Abertos do Departamento de Bioengeharia
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/12/12 - 2016/12/16 EMBL Course - Genome Engineering: CRISPR/Cas9
Workshop
Genome Engineering: CRISPR/Cas9
European Molecular Biology Laboratory, Germany
2011/08/16 - 2011/08/24 FEBS Summer School: Molecular Mechanisms in Signal Transduction and Cancer
Conference

Jury of academic degree

Topic
Role
Candidate name (Type of degree)
Institution / Organization
2023/11/27 A critical role for macrophages in tissue homeostasis maintenance
Thesis Member
Maria Ramos Bárbara de Vasconcelos Pires (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2023/11 Unravelling the Complex Epigenome Regulation by Vitamin C During Development Across Diverse Genetic Backgrounds
(Thesis) Arguer
Luís Pereira Pina (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2023/11 Modelling Angelman Syndrome in CRISPR-edited isogenic iPSCs
(Thesis) Arguer
Alípio Paulo Vasco Parreirão e Gomes (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2023/07/04 Evaluating the requirement of Nucleolin N-terminal domain to suppress TDP-43 toxicity in HEK293T cells
(Thesis) Arguer
Daniel António Ramiro Pereira (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2022 Exploitation of Burkholderia cenocepacia J2315 surface-exposed proteins as targets for immunotherapeutic approaches against infections
(Thesis) Arguer
Carolina Simões Gonçalves Silva (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2022 Exploring the protective role of bacterial components in cancer development
(Thesis) Arguer
Inês Pontes Gomes (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2022 Using CRISPR-Cas9 to generate an isogenic cell line from patient-derived human induced pluripotent stem cells to model Niemann-Pick Type C1 Cerebellar Ataxia
(Thesis) Arguer
João Rafael Camões dos Santos (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2022 Furan-based PROTACs as predators of GPCR proteins
(Thesis) Arguer
Sebastião Viegas Tavares Ferreira de Almeida (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/09/24 The anticancer potential of the bacterial protein azurin and its derived peptides: mode of action and development of a nanosized drug delivery system for lung cancer therapy
Thesis Member
Ana Rita Cebola Garizo (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/06/18 Genetic modulation of pediatric cerebral vasculopathy in sickle cell anemia
(Thesis) Arguer
Sandra Marisa Duarte da Silva (PhD)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/01 Establishment of 293 based AAV packaging cell lines using shRNA-mediated rep silencing
(Thesis) Main arguer
Joana Filipa Gaspar Diogo (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2021/01 Investigation of the Role of Base Excision Repair Gene Xrcc1 in Hematopoietic Progenitor and Stem Cells
(Thesis) Main arguer
Beatriz Guapo Neves (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2020/12 Characterization of anti-Pneumococcal surface protein C affibodies using surface plasmon resonance technology
(Thesis) Main arguer
Beatriz Silva Pedroso (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019 Engineering of smart exosomes for clearance of Abeta amyloid protein in Alzheimer’s disease
(Thesis) Arguer
Joana Martins Machado (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019 Studying the molecular interactions of fibrinogen with erythrocytes in carotid artery disease
(Thesis) Arguer
Sofia Antunes Angélico Pinto Nabais (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2019 Development of 3D spheroid cultures of cancer cells for the preclinical evaluation of novel theranostic agents
(Thesis) Arguer
Catarina Isabel Guilherme Pinto (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018 RNAi as a tool to inhibit the angiogenic potential of human Mesenchymal Stem/Stromal Cells in malignancy
(Thesis) Arguer
Cristiana da Silva Ulpiano (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018 Deciphering the identity of oral and gut bacteria in colorectal cancer
(Thesis) Arguer
Lisa Alves Azevedo (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018 Study of the Mechanisms of Chromosome Segregation in the C. elegans Embryo
(Thesis) Arguer
Ana Miguel Dias Maia Henriques (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2018 Generating isogenic NPC1 hiPSC lines for disease modelling using the CRISPR/Cas9 system
(Thesis) Arguer
Joaquim Filipe Aresta Lopes Godinho (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/11/21 Genome Editing via CRISPR/Cas9 Targeted Integration in CHO cells
(Thesis) Arguer
Catarina Andreia Martins Freire (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017/11/17 Effects of honey (Revamil®) on macrophages and LPS-treated cells: Impact on the biology of mitochondria and lysosomes
(Thesis) Arguer
Cláudia Margarida de Jesus Esteves (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 Evaluating the impact of culture conditions on human mesenchymal stem/stromal cell-derived exosomes through FTIR spectroscopy
(Thesis) Arguer
Maria João Canha Pereira (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2017 Investigating Arx role in mushroom body development in Platynereis dumerilii
(Thesis) Arguer
Joaquim António Da Silva Melo Contradanças (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/12/09 Hypoxia Gene-expression Signature for Breast Cancer: Patient Survival Prediction and New Hypoxia-genes
(Thesis) Arguer
João Miguel Soeima Brás (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/11/30 Using CRISPR-Cas technology to generate inducible KO mouse ES cell lines for Ago1 and Ago3
(Thesis) Arguer
Ariana Mónica Silva Peredo (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/11/21 Plant Extract Counteracts Detrimental Effects of Cellular Senescence in Human Skin Fibroblasts
(Thesis) Arguer
André Dargen de Matos Branco (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2016/10/21 Genome-wide profiling of alternative splicing in aging across human tissues: Tissue-specific aging signature and contribution of cellular senescence
(Thesis) Arguer
Ana Carolina de Sousa Leote (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2015/11/20 Knockdown of Monocyte Chemotactic Protein 1 in human mesenchymal stromal cells using two different approaches: shRNA and CRISPR Cas 9
(Thesis) Arguer
Matilde Roquete Viana Neto (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014/12/01 Phenotypic Signature of Drug Response in Prostate Cancer Cells
(Thesis) Arguer
Leonor Gabriel Guedes da Silva (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal
2014/06/20 E-cadherin mutations, cell cycle dynamics and morphology
(Thesis) Arguer
Cátia Inês Durão Ferreira (Master)
Universidade de Lisboa Instituto Superior Técnico, Portugal

Association member

Society Organization name Role
2018/01/01 - Current ASPIC - Portuguese association for cancer researc Member
2016/01/01 - Current Sociedade Portuguesa de Biotecnologia Member

Committee member

Activity description
Role
Institution / Organization
2023/01/01 - Current Member of iBB Communication&Outreach Team
Member
Universidade de Lisboa Instituto de Bioengenharia e Biociências, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2019/11/01 - Current Genetic Engineering - Lab Course in Gene Editing of human cells with CRISPR/Cas9 technology Engenharia Biológica (Mestrado integrado) Universidade de Lisboa Instituto Superior Técnico, Portugal
2019/10/01 - Current Molecular Biotechnology - Lab Course in Gene Editing of human cells with CRISPR/Cas9 technology Biotecnologia (Mestrado) Universidade de Lisboa Instituto Superior Técnico, Portugal
2022/04/01 - 2022/06/30 Biochemistry and Molecular Biology of the Cell Engenharia Biológica (Licenciatura) Universidade de Lisboa Instituto Superior Técnico, Portugal

Evaluation committee

Activity description
Role
Institution / Organization Funding entity
2024/02/06 - 2024/02/06 Comissão de Acompanhamento de Tese de Doutoramento de Isabel Doutor
Member
Universidade de Lisboa Instituto Superior Técnico, Portugal
2024/01/18 - 2024/01/18 Comissão de Acompanhamento de Tese de Doutoramento de Adriana Cruz
Member
Universidade de Lisboa Instituto Superior Técnico, Portugal
2022/04 - 2022/04 Comissão de Acompanhamento de Tese de Doutoramento de Cristiana Ulpiano
Member
Universidade de Lisboa Instituto Superior Técnico, Portugal
2020/12 - 2020/12 Comissão de Acompanhamento de Tese de Doutoramento de Ana Rita Cebola Garizo
Member
Universidade de Lisboa Instituto Superior Técnico, Portugal
Distinctions

Award

2022 Prémio Excelência na Docência
Universidade de Lisboa Instituto Superior Técnico, Portugal
2021 Prémio Excelência na Docência
Universidade de Lisboa Instituto Superior Técnico, Portugal
2020 Fulbright Visiting Scholar Fellowship
Fulbright Portugal, Portugal
2018 EACR Best Poster Prize ASPIC 2018
European Association For Cancer Research, United Kingdom
2016 Participation in the course: Genome Engineering: CRIPRS/Cas9 (Travel and Meeting Expenses)
Boehringer Ingelheim International GmbH, Germany
2009 CLUSTER Prize 2009
Universidade de Lisboa Instituto Superior Técnico, Portugal