Mário Santos (3819-E213-C7F4)

Curriculum Vitae

Mário Santos

Mário Santos concluded his PhD in Biotechnology in 2014, a master in Biological engineering in 2008, and bachelor in Biological sciences in 2007, all at Instituto Superior Técnico, Universidade de Lisboa. His PhD studies focused on the symbiotic interactions between Sinorhizobium meliloti and the host leguminous plants Medicago sativa and Medicago truncatula, namely the biochemical characterization of the transcriptional regulator EmrR and the major facilitator superfamily transporter EmrA/EmrB from S. meliloti (Santos et al. 2014). During his PhD he co-supervised 3 Master theses and 5 undergraduate students. After his PhD, he started his current position as lab manager of the Plant Genomics group, at the Instituto Gulbenkian de Ciência (IGC), under the supervision of Dr. Jörg Becker. His tasks include the organization of the laboratory, taking care of orders and stocks, as well as preparing all the necessary reagents and material. Moreover, he has been involved in a number of ongoing projects in the group (e.g., Pereira et al. 2020). He has also been directly involved in the development and optimization of FACS protocols to isolate sperm cells and vegetative nuclei from Arabidopsis thaliana (Santos et al. 2017 and Misra et al. 2019). He is also a part of a collaboration between the Plant Genomics group and the Flow Cytometry and Advanced Imaging Units at IGC, aiming at the development of new methods for sorting of gametes in angiosperms. He has also been part of the organizing team for the EMBO workshop “New shores in land plant evolution” and for a small meeting for the Mosstech project. During his scientific career he has published 5 articles in international peer-reviewed journals and has co-authored two book chapters. In addition he has been participating regularly in various outreach activities, communicating science to the general public. These include, amongst others, Inspirar Ciência 2018, a workshop on Plant Biology for high school teachers, IGC Open day, and Science Alive - IGC stand at the 2019 NOS Alive Music Festival.

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Identification

Personal identification

Full name: Mário Santos

Citation names

Santos, Mário
Santos MR

Author identifiers

Ciência ID: 3819-E213-C7F4
ORCID iD: 0000-0001-5612-6620

Email addresses

mrsantos@igc.gulbenkian.pt (Professional)
marioalmeidasantos@gmail.com (Personal)

Telephones

Telephone

214464689 (Professional)

Addresses

Instituto Gulbenkian de Ciência. Plant Genomics Lab - Rua Quinta Grande Nº6, 2780-156, Oeiras, Oeiras, Portugal (Professional)

Websites

https://gulbenkian.pt/ciencia/research/research-groups/plant-genomics/ (Professional)

Knowledge fields

Natural sciences - Biological Sciences - Plant sciences
Natural sciences - Biological Sciences - Microbiology
Natural sciences - Biological Sciences - Microbiology

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Portuguese (Mother tongue)
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)

Education

	Degree	Classification
2009/10/01 - 2014/09/22 Concluded	Biotecnologia (Doutoramento) Major in Sem especialidade Universidade de Lisboa Instituto Superior Técnico, Portugal "Sinorhizobium meliloti outer membrane protein TolC and the transcriptional repressor EmrR are bacterial determinants of root-nodule symbiosis " (THESIS/DISSERTATION)	Aprovado com Muito Bom
2008 Concluded	Mestrado Integrado em Engenharia Biológica (Mestrado) Universidade de Lisboa Instituto Superior Técnico, Portugal "Assessment of the role of proteins with Ca2+- binding repeats in the symbiosis between Sinorhizobium meliloti and Medicago sativa" (THESIS/DISSERTATION)	17
2007 Concluded	Licenciatura em Ciências de Engenharia Biológica (Licenciatura) Universidade de Lisboa Instituto Superior Técnico, Portugal "Not applicable " (THESIS/DISSERTATION)	14

Affiliation

Science

	Category Host institution	Employer
2020/10/26 - Current	Contracted Researcher (Research)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Others

	Category Host institution	Employer
2015/04/01 - 2020/10/15	Research Technician - Lab manager	Instituto Gulbenkian de Ciência, Portugal

2013/10/01 - 2014/09/30	Research fellow in the project “KINASE- Phosphoproteomics in Burkholderia to assess the role of tyrosine phosphorylation in virulence determinants expression.” PTDC/QUI-BIQ/118260/2010	Universidade de Lisboa Instituto Superior Técnico, Portugal

2009/10/01 - 2013/10/01	PhD fellowship (SFRH/BD/60466/2009) financed by Fundação para a Ciência e Tecnologia, Instituto Superior Técnico,Lisboa, Portugal	Universidade de Lisboa Instituto Superior Técnico, Portugal

2008/10/01 - 2009/09/30	Research fellow in the project “Role of exopolysaccharides in the early steps of Rhizobium-legume symbiosis” PTDC/AGR-AAM/66977/2006	Universidade de Lisboa Instituto Superior Técnico, Portugal

2002/09/15 - 2008/08/30	MSc in Biological Engineering, Instituto Superior Técnico, Universidade Técnica de Lisboa, Portugal	Universidade de Lisboa Instituto Superior Técnico, Portugal

Projects

Grant

	Designation	Funders
2015/04 - 2019/09	Unidade de I&D 4555 IGC ref--LAB1M5 Unidade de I&D 4555 IGC ref--LAB1M5	Fundação para a Ciência e a Tecnologia
2013/09/01 - 2014/08/31	KINASE- Phosphoproteomics in Burkholderia to assess the role of tyrosine phosphorylation in virulence determinants expression info:eu-repo/grantAgreement/FCT/3599-PPCDT/118260/PT Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2009/10/01 - 2013/09/30	Biochemical characterization of the transcriptional regulator EmrR and the major facilitator superfamily transporter EmrA/EmrB from S. meliloti SFRH/BD/60466/2009 PhD Student Fellow Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia
2009 - 2012	ROOT-INT- Role of a two-component regulatory system in the early interaction between Sinorhizobium meliloti and plant root hairs. PTDC/BIA-MIC/113733/2009 Master Student Fellow	Concluded
2008/10 - 2009/10	Função dos exopolissacáridos nos passos iniciais da simbiose entre Rhizobium e plantas leguminosas PTDC/AGR-AAM/66977/2006	undação para a Ciência e Tecnologia, Instituto Superior Técnico
2008/10/01 - 2009/09/30	Role of the exopolysaccharides in the early steps of Rhizobium-legume symbiosis info:eu-repo/grantAgreement/FCT/3599-PPCDT/66977/PT Master Student Fellow Universidade de Lisboa Instituto Superior Técnico, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Other

	Designation	Funders
2017 - Current	Heterogeneous expression in isomorphic male gametes - a single cell transcriptomics approach to explore preferential fertilization in Arabidopsis thaliana PTDC/BIA-FBT/28484/2017 Researcher Instituto Gulbenkian de Ciência, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2015/11/01 - 2019	14 ERA-CAPS. EVOREPRO. Evolution of Sexual Reproduction in Plants info:eu-repo/grantAgreement/RCUK/BBSRC/BB2%FN0050902%F1/GB Researcher	Fundação para a Ciência e a Tecnologia Concluded
2004/09/01 - 2006/09/01	Expression profiling studies to specify the symbiotic genetic programme of the model legume Medicago truncatula info:eu-repo/grantAgreement/FCT/3599-PPCDT/70592/PT Other	Fundação para a Ciência e a Tecnologia

Outputs

Publications

Book chapter	Santos, Mário R.; Bispo, Cláudia; Becker, Jörg D.. "Isolation of Arabidopsis Pollen, Sperm Cells, and Vegetative Nuclei by Fluorescence-Activated Cell Sorting (FACS)". In Methods in Molecular Biology, 193-210. Springer New York, 2017. Published • 10.1007/978-1-4939-7286-9_16 Moreira, Leonilde M.; Silva, Inês N.; Ferreira, Ana S.; Santos, Mário R.. "Stress Conditions Triggering Mucoid-to-nonmucoid Morphotype Variation inBurkholderia, and Effects on Virulence and Biofilm Formation". In Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria, edited by Frans J. de Bruijn, 1295-1303. John Wiley & Sons, Inc., 2016. Published • 10.1002/9781119004813.ch126
Conference abstract	Silva Ines Nunes; Santos PM; Santos MR; Buskirk S; Zlosnik JE; Speert DP; Cooper VS; Moreira, Leonilde M. "Genetic variation analysis of Burkholderia multivorans during long-term colonization of a cystic fibrosis patient". Paper presented in International Burkholderia cepacia working group (IBCWG), Vancouver, 2015. Published Santos MR. "Fixação biológica de azoto". Paper presented in Laboratórios Abertos do Departamento de Bioengenharia, lisboa, 2013. Published Santos MR; Marques, AT; Becker, J; Moreira, Leonilde M. "Involvement of the TetR transcriptional regulator SMc03169 in pH sensitivity and contribution to nodulation competitiveness in Sinorhizobium meliloti". Paper presented in 3rd Scientific Meeting of the Institute for Biotechnology and Bioengineering. Lisboa, Portugal., 2010. Santos MR; Marques, AT; Becker, J; Moreira, Leonilde M. "New insights in the molecular events underlying Medicago-Sinorhizobium biological nitrogen fixation symbiosis". Paper presented in 2nd Scientific Meeting of the Institute for Biotechnology and Bioengineering. Braga, Portugal., 2010. Santos MR; Cosme AM; Mata, M; Medeiros, M; Moreira, Leonilde M. "Bacterial determinats of the nitrogen fixation symbiosis between Sinorhizobium meliloti and leguminous plants". Paper presented in 1st Scientific Meeting of the Institute for Biotechnology and Bioengineering. Faro, Portugal., 2009.
Conference poster	Rafael-Fernandes M; Nuno P. Martins; Santos MR; Misra CS; Becker, J; Monteiro M.. "Cell sorting and imaging of Arabidopsis thaliana sperm cells and vegetative nuclei without fluorescence". Paper presented in 34th Congress of the International Society for Advancement of Cytometry, 2019. Rafael-Fernandes M; Martins NP; Santos MR; Chandra S. Misra; Bispo, C; Becker, J; Monteiro M.. "FACS isolation and imaging of Arabidopsis thaliana sperm cells and vegetative nuclei". Paper presented in 33th Congress of the International Society for Advancement of Cytometry, 2018. Santos MR; Marques, AT; Becker, J; Moreira, Leonilde M. "Comparative transcriptomic analyses of the SMc03169 mutant from Sinorhizobium meliloti and its role in symbiosis with leguminous plants". Paper presented in 10th European Nitrogen Fixation Congress (ENFC), 2012. Marques, AT; Santos MR; Becker, J; Moreira, Leonilde M. "Characterization of the TetR transcriptional regulator SMc03169 and its contribution to symbiosis between Sinorhizobium meliloti and leguminous plants.". Paper presented in XXXVII Portuguese Genetics Conference, 2012. Santos MR; Marques, AT; Becker, J; Moreira, Leonilde M. "Role of the Cpx two component regulatory system and a MFS transporter in the symbiosis between Sinorhizobium meliloti and leguminous plant". Paper presented in 17th International Congress on Nitrogen Fixation, 2011. Santos MR; Cosme AM; Medeiros, J; Mata, M; Moreira, Leonilde M. "Bacterial determinats of the nitrogen fixation symbiosis between Sinorhizobium meliloti and leguminous plants". Paper presented in I Encontro IBB, 2009. Santos MR; Medeiros, J; Mata, M; Moreira, Leonilde M. "Role of a two-component regulatory system from Sinorhizobium meliloti in adhesion to plant roots". Paper presented in Microbiotec09 congress, 2009. Santos MR; Cosme AM; Moreira, Leonilde M. "Assessment of the role of proteins with Ca2+-binding repeats in the symbiosis between Sinorhizobium meliloti and Medicago sativa". Paper presented in XVI Congresso Nacional de Bioquímica., 2008. Cosme AM; Santos MR; Becker, J; Moreira, Leonilde M. "TolC from Sinorhizobium meliloti is involved in the secretion of several molecules relevant for the symbiosis with legume plants". Paper presented in XVI Congresso Nacional de Bioquímica, 2008. Cosme AM; Santos MR; Becker, J; Sharypova LA; Santos PM; Moreira, Leonilde M. "The outer membrane protein TolC from Sinorhizobium meliloti affects protein secretion, polysaccharide biosynthesis, antimicrobial resistance, and symbiosis". Paper presented in 8th European Nitrogen Fixation Conference, 2008. Ferreira, AS; Leitão JH; Sousa, Silvia; Cosme AM; Santos MR; Sá-Correia I; Moreira, Leonilde M. "A protein tyrosine kinase and a phosphotyrosine phosphatase from Burkholderia cepacia are associated with virulence and production of exopolysaccharide". Paper presented in 2nd FEMS Congress of European Microbiologists, 2006.
Journal article	Patrícia A. Pereira; Leonor C. Boavida; Mário R. Santos; Jörg D. Becker; Pereira, P. A; Boavida, L. C; Santos MR; Becker, J. "AtNOT1 is required for gametophyte development in Arabidopsis". The Plant Journal 103 4 (2020): 1289-1303. https://doi.org/10.1111/tpj.14801. Accepted • 10.1111/tpj.14801 Chandra Shekhar Misra; Mário R. Santos; Mariana Rafael-Fernandes; Nuno P. Martins; Marta Monteiro; Jörg D. Becker. "Transcriptomics of Arabidopsis sperm cells at single-cell resolution". Plant Reproduction 32 1 (2019): 29-38. https://doi.org/10.1007/s00497-018-00355-4. 10.1007/s00497-018-00355-4 Silva, Inês N.; Pessoa, Filipa D.; Ramires, Marcelo J.; Santos, Mário R.; Becker, Jörg D.; Cooper, Vaughn S.; Moreira, Leonilde M.. "The OmpR Regulator of Burkholderia multivorans Controls Mucoid-to-Nonmucoid Transition and Other Cell Envelope Properties Associated with Persistence in the Cystic Fibrosis Lung". Journal of Bacteriology 200 17 (2018): http://dx.doi.org/10.1128/jb.00216-18. 10.1128/jb.00216-18 Silva, Inês N.; Santos, Pedro M.; Santos, Mário R.; Zlosnik, James E. A.; Speert, David P.; Buskirk, Sean W.; Bruger, Eric L.; et al. "Long-Term Evolution of Burkholderia multivorans during a Chronic Cystic Fibrosis Infection Reveals Shifting Forces of Selection". mSystems 1 3 (2016): http://dx.doi.org/10.1128/msystems.00029-16. 10.1128/msystems.00029-16 Santos, M.R.; Marques, A.T.; Becker, J.D.; Moreira, L.M.. "The sinorhizobium meliloti emrr regulator is required for efficient colonization of medicago sativa root nodules". Molecular Plant-Microbe Interactions 27 4 (2014): 388-399. http://www.scopus.com/inward/record.url?eid=2-s2.0-84896802655&partnerID=MN8TOARS. 10.1094/MPMI-09-13-0284-R Santos, Mario R; Cosme, Ana M; Becker, Jorg D; Medeiros, Joao M C; Mata, Marcia F; Moreira, Leonilde M. "Absence of functional TolC protein causes increased stress response gene expression in Sinorhizobium meliloti". BMC Microbiology 10 1 (2010): 180. http://dx.doi.org/10.1186/1471-2180-10-180. 10.1186/1471-2180-10-180 Cosme, A.M.; Becker, A.; Santos, M.R.; Sharypova, L.A.; Santos, P.M.; Moreira, L.M.. "The outer membrane protein TolC from Sinorhizobium meliloti affects protein secretion, polysaccharide biosynthesis, antimicrobial resistance, and symbiosis". Molecular Plant-Microbe Interactions 21 7 (2008): 947-957. http://www.scopus.com/inward/record.url?eid=2-s2.0-47549111221&partnerID=MN8TOARS. 10.1094/MPMI-21-7-0947
Thesis / Dissertation	Santos MR. "Sinorhizobium meliloti outer membrane protein TolC and the transcriptional repressor EmrR are bacterial determinants of root-nodule symbiosis". PhD, Universidade de Lisboa Instituto Superior Técnico, 2014. Santos MR. "Assessment of the role of proteins with Ca2+- binding repeats in the symbiosis between Sinorhizobium meliloti and Medicago sativa". Master, Universidade de Lisboa Instituto Superior Técnico, 2008.

Activities

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2013 - 2013	Functional analysis of RTX proteins of Sinorhizobium meliloti and role in biological nitrogen fixation symbiosis Co-supervisor	Mestrado em Microbiologia Aplicada (Master) Fundação da Faculdade de Ciências da Universidade de Lisboa, Portugal
2011 - 2011	Role of a tripartite efflux pump in the symbiosis betweem Sinorhizobium Meliloti and Leguminous plants Co-supervisor	Mestrado em Microbiologia Aplicada (Master) Universidade de Lisboa Faculdade de Ciências, Portugal

Event organisation

	Event name Type of event (Role)	Institution / Organization
2018/09/26 - 2018/09/28	Mosstech "Idea to Product" course (2018/09/26 - 2018/09/28) Workshop (Member of the Organising Committee)	Instituto Gulbenkian de Ciência, Portugal
2018 - 2018	Organization of the EMBO workshop "New shores in land plant evolution", Lisbon, Portugal (2018 - 2018) Congress (Member of the Organising Committee)	Instituto Gulbenkian de Ciência, Portugal

Mentoring / Tutoring

	Topic	Student name
2012 - 2012	MSc in Bioengineering	Flávia Encarnação
2011 - 2011	Functional analysis of the metalloprotease SMa0034 from Sinorhizobium meliloti	Jõao Medeiros
2011 - 2011	MSc in Bioengineering	Daniela Isidoro
2010 - 2010	Pesquisa de novos determinantes genéticos que contribuem para a simbiose entre Sinorhizobium e plantas leguminosas	Mafalda Santos
2009 - 2009	Functional analysis of the metalloprotease SMa0034 from Sinorhizobium meliloti	Márcia Mata
2009 - 2009	MSc in Bioengineering and Nanotechnology	Ana Navarro Rodrigues