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Identification

Personal identification

Full name
Daniel Alexandre Sousa Henriques

Citation names

  • Henriques, Daniel

Author identifiers

Ciência ID
3B13-D28E-4169
ORCID iD
0000-0001-8189-6918

Knowledge fields

  • Natural sciences - Biological Sciences - Biology
  • Natural sciences - Biological Sciences - Cell Biology
  • Natural sciences - Biological Sciences - Molecular Biology
  • Medical and Health Sciences - Basic Medicine - Neurosciences
  • Medical and Health Sciences - Medical Biotechnology - Technologies Involving the Manipulation of Cells, Tissues, Organs or the Whole Organism
  • Natural sciences - Biological Sciences - Genetics and Heredity
  • Natural sciences - Biological Sciences - Biochemical Research Methods
  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Portuguese (Mother tongue)
Education
Degree Classification
2020/11 - 2024
Ongoing
Experimental Biology and Biomedicine (BEB) (Doutoramento)
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
"On the modulation of adult brain plasticity – a strategy to improve human neuroepithelial stem cells migration, differentiation, and functional integration upon cerebellar transplantation in Machado-Joseph disease" (THESIS/DISSERTATION)
2021 - 2022
Concluded
V Modular Structure training in Laboratory Animal Science (Outros)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2018 - 2018
Concluded
Gestão de Resíduos Hospitalares Perigosos - Triagem e Acondicionamento; Ambiwaste, Portugal (Outros)
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2018
Concluded
MSc in Cellular and Molecular Biology (Mestrado)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"IPSCS-DERIVED NESCS FOR NEUROREGENERATION IN MACHADO-JOSEPH DISEASE." (THESIS/DISSERTATION)
18
2016
Concluded
BSc in Biology (Licenciatura)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"N/a" (THESIS/DISSERTATION)
16
2012/05 - 2012/05
Concluded
Certificate in Advanced English (Outros)
Major in English Language
International House Coimbra, Portugal
2010 - 2010
Concluded
First Certificate in English (FCE) (Outros)
Major in English Language
International House Coimbra, Portugal
Affiliation

Science

Category
Host institution
Employer
2020/11 - 2024/10 Researcher (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2020/08 - 2020/10 Researcher (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2020/01/02 - 2020/07/02 Researcher (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2019/02/11 - 2019/08/10 Researcher (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2017/07/02 - 2018/08/20 Research Trainee (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2017/06/05 - 2017/06/30 Research Trainee (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2017/05/08 - 2017/06/02 Research Trainee (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2017/01/30 - 2017/02/24 Research Trainee (Research) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Projects

Grant

Designation Funders
2020/01/02 - 2020/07/02 The impact of induced pluripotent stem cells-derived neuroepithelial stem cells transplantation in Machado-Joseph disease
POCI-01-0145-FEDER-030737
Research Fellow
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia
2019/02/11 - 2019/08/10 BrainHealth 2020: Deteção Precoce, Neuromodulação e Terapias Avançadas para Neuropatologias
CENTRO-01-0145-FEDER-000008
Research Fellow
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Outputs

Publications

Book chapter
  1. Henriques, Daniel; Boltze, Johannes; Pereira de Almeida, Luis ; Mendonça, Liliana. "Strategies to Upgrade the Stem Cell Application for Brain Transplantation". In Stem Cell Biology and Regenerative Medicine, 27-58. Springer International Publishing, 2024.
    10.1007/978-3-031-49744-5_2
Conference poster
  1. Moreira, Ricardo; Henriques, Daniel; Nóbrega, Clévio; Pereira de Almeida, Luis; Liliana Mendonça. "Enhancing migration and differentiation of IPSC-derived Neuroepithelial Stem cells for cell replacement therapies in Machado Joseph Disease". Paper presented in ETRS & SPCE-TC 2023 Joint Annual Meeting, 2023.
  2. AR Fernandes; D Adão; SM Lopes; Henriques, Daniel; F Pena; M Dias; MM Santana; P Perdigão; LP de Almeida. "Engineering isogenic neuronal models of ATXN3 knockout in Machado-Joseph Disease patient cells by CRISPR/Cas9.". Paper presented in ESGCT 30th Annual Congress, 2023.
  3. Henriques, Daniel; Ricardo Moreira; Pedro Perdigão; Agnete Kirkeby; Pereira de Almeida, L; Liliana S. Mendonça. "Induced pluripotent stem cell-derived Cerebellar Neuronal Progenitors for transplantation in Machado-Joseph Disease". Paper presented in FENS Regional meeting 2023, 2023.
  4. Moreira, Ricardo; Henriques, Daniel; Nóbrega, Clévio; Pereira de Almeida, Luis ; Liliana Mendonça. "Modulation of migration and neuronal differentiation of iPSC-derived Neuroepithelial Stem Cells to improve cell replacement strategies in Machado-Joseph Disease". Paper presented in FENS Regional meeting 2023, 2023.
  5. Henriques, Daniel; Moreira, Ricardo; Pedro Perdigão; Agnete Kirkeby; Pereira de Almeida, Luis ; Mendonça, Liliana S. "Generation and characterization of cerebellar neuronal progenitors for Machado-Joseph Disease treatment". Paper presented in The 29th Annual Meeting of the European Society of Gene & Cell Therapy – ESGCT 2022, 2022.
  6. Moreira, Ricardo; Henriques, Daniel; Nóbrega, Clévio; Pereira de Almeida, Luis ; Liliana Mendonça. "Modulation of migration and neuronal differentiation of iPSC-derived Neuroepithelial Stem Cells to improve cell therapy in Machado-Joseph Disease". Paper presented in The 29th Annual Meeting of the European Society of Gene & Cell Therapy – ESGCT 2022, 2022.
  7. Daniel Henriques; Moreira, Ricardo; Pedro Perdigão; Agnete Kirkeby; Luís Pereira de Almeida; Liliana Mendonça. Corresponding author: Daniel Henriques. "Generation of cerebellar neuronal progenitors for cell replacement strategies in Machado-Joseph Disease". Paper presented in XVII Meeting of the Portuguese Society for Neuroscience, 2021.
  8. Braz, Sandra O.; Catarina Vinhas; Daniel Henriques; Ricardo Moreira; Rosário Faro; Sónia Duarte; Luís Pereira de Almeida; Liliana S. Mendonça. "Cerebellar transplantation of iPSC-derived neuroepithelial stem cells silenced for mutant ATXN3 for Machado-Joseph disease". Paper presented in XVII Meeting of the Portuguese Society for Neuroscience, 2021.
  9. Liliana S. Mendonça; Daniel Henriques; Vanessa Fernandes; Ricardo Moreira; Ana C. Vinhas; Rosário Faro; Sónia Duarte; Jens C. Schwamborn; Luís Pereira de Almeida. "Patient specific IPSC-derived neuroepithelial stem cells for cell-based therapy in Machado-Joseph disease". Paper presented in FENS 2020 Virtual Forum, 2020.
  10. Moreira, Ricardo; Liliana S. Mendonça; Daniel Henriques; Sónia Duarte; Teresa Rodrigues; Henrique Girão; Jens C. Schwamborn; Luís Pereira de Almeida. "Investigation of Mutant Ataxin-3 spreading in Machado-Joseph Disease". Paper presented in 1st Annual Meeting in Pharmaceutical Sciences (Coimbra, Portugal), 2019.
  11. Ricardo Moreira; Liliana S. Mendonça; Daniel Henriques; Sónia Duarte; Teresa Rodrigues; Henrique Girão; Jens C. Schwamborn,; Luís Pereira de Almeida. "Investigation of Mutant Ataxin-3 spreading in Machado-Joseph Disease". Paper presented in XVI Meeting of the Portuguese Society for Neuroscience (Lisbon, Portugal), 2019.
  12. Liliana S. Mendonça; Henriques, Daniel; Ricardo Moreira; Sónia Duarte; Jens C. Schwamborn; Luís Pereira de Almeida. "Generation and transplantation of patient-specific iPSC-derived neuroepithelial stem cells for Machado-Joseph disease treatment". Paper presented in European Society of Gene and Cell Therapy congress (Lausanne, Switzerland), 2018.
Journal article
  1. Mendonça, Liliana S.; Henriques, Daniel; Fernandes, Vanessa; Moreira, Ricardo; Brás, João; Duarte, Sónia; Schwamborn, Jens C.; Pereira de Almeida, Luis. "Graft-derived neurons and bystander effects are maintained for six months after human iPSC-derived NESC transplantation in mice’s cerebella". Scientific Reports 14 1 (2024): http://dx.doi.org/10.1038/s41598-024-53542-x.
    10.1038/s41598-024-53542-x
  2. Brás, João; Henriques, Daniel; Moreira, Ricardo; Santana, Magda M.; Silva-Pedrosa, Rita; Adão, Diana; Braz, Sandra; et al. "Establishment and characterization of human pluripotent stem cells-derived brain organoids to model cerebellar diseases". Scientific Reports 12 1 (2022): http://dx.doi.org/10.1038/s41598-022-16369-y ; Impact factor at time of publication: 4.996 (2022).
    10.1038/s41598-022-16369-y
  3. Henriques, Daniel; Moreira, Ricardo; Schwamborn, Jens; Pereira de Almeida, Luís; Mendonça, Liliana S.. "Successes and Hurdles in Stem Cells Application and Production for Brain Transplantation". Frontiers in Neuroscience 13 (2019): http://dx.doi.org/10.3389/fnins.2019.01194; Impact factor at time of publication: 4.154 (2019).
    Published • 10.3389/fnins.2019.01194
  4. Mendonça, Liliana S; Nóbrega, Clévio; Tavino, Silvia; Brinkhaus, Maximilian; Matos, Carlos; Tomé, Sandra; Moreira, Ricardo; et al. "Ibuprofen enhances synaptic function and neural progenitors proliferation markers and improves neuropathology and motor coordination in Machado-Joseph disease models". Human Molecular Genetics 28 22 (2019): 3691-3703. http://dx.doi.org/10.1093/hmg/ddz097; Impact Factor at time of publication: 5.1 (2019).
    Published • 10.1093/hmg/ddz097
Newspaper article
  1. Ana Catarina Vinhas; Ana Cristina Ferreira; Carina Henriques; Carolina Silva; Catarina Miranda; Daniel Henriques; Daniela Oliveira; et al. "Uma Viagem Rara", PÚBLICO, 2020, https://www.publico.pt/2020/02/28/ciencia/fotogaleria/viagem-rara-400481..
Thesis / Dissertation
  1. Henriques, Daniel. "IPSCs-derived NESCs for neuroregeneration in Machado-Joseph Disease". Master, Universidade de Coimbra Faculdade de Ciencias e Tecnologia, 2018.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/02 In vitro drug-mediated modulation of stem cell migration and differentiation for improved cell replacement strategies 54 th Meeting of the Portuguese Society of Pharmacology, 42 nd Meeting of Clinical Pharmacology and 23 rd Meeting of Toxicology
(Lisboa, Portugal)
2024/02 Brain-targeted liposomes encapsulating a differentiationinducing drug enhance neural differentiation of human iPSCderived neuroepithelial stem cells 54 th Meeting of the Portuguese Society of Pharmacology, 42 nd Meeting of Clinical Pharmacology and 23 rd Meeting of Toxicology
(Lisboa, Portugal)
2023/10 Generating induced pluripotent stem cell-derived cerebellar neuronal progenitors for cell transplantation in Machado-Joseph Disease ETRS & SPCE-TC 2023 Joint Annual Meeting
(Coimbra, Portugal)
2023/02 Cerebellar Neuronal Pregenitors for cerebellar transplantation in Machado-Joseph Disease 53rd Meeting of the Portuguese Society of Pharmacology, 41st Meeting of Clinical Pharmacology and 22nd Meeting of Toxicology
Instituto de Farmacologia e Terapêutica Experimental, Faculdade de Medicina da Universidade de Coimbra (Coimbra, Portugal)
2023/02 Pharmacological modulation of migration and neuronal differentiation of IPSC-derived neuroepithelial stem cells 53rd Meeting of the Portuguese Society of Pharmacology, 41st Meeting of Clinical Pharmacology and 22nd Meeting of Toxicology
Instituto de Farmacologia e Terapêutica Experimental, Faculdade de Medicina da Universidade de Coimbra (Coimbra, Portugal)
2021/02/19 iPSC-derived neuroepithelial stem cells depleted for mutant ataxin-3 for Machado-Joseph disease treatment LI Annual meeting of the Portuguese Pharmacology Society, XXXIX Clinical Pharmacology meeting and XX meeting of Toxicology
Faculty of Pharmacy, University of Lisbon (Lisbon, Portugal)
2020/02/07 Establishment and characterization of human pluripotent stem cells derived brain organoids L Annual meeting of the Portuguese Pharmacology Society, XXXVIII Clinical Pharmacology meeting and XIX meeting of Toxicology
(Coimbra, Portugal)
2020/02/06 Human IPSC-derived neuroepithelial stem cells for cell-based therapy in Machado-Joseph Disease L Annual meeting of the Portuguese Pharmacology Society, XXXVIII Clinical Pharmacology meeting and XIX meeting of Toxicology
(Coimbra, Portugal)
2019/11/15 Human iPSC-derived neuroepithelial stem cells as a source for cell therapy in Machado-Joseph disease 1st Annual Meeting in Pharmaceutical Sciences. Faculty of Pharmacy of the University of Coimbra
(Coimbra, Portugal)
2019/05/30 Human iPSC-derived Neuroepithelial stem cells from cell-based therapies in Machado-Joseph disease VI Meeting of the Portuguese Society for Neuroscience
Portuguese Society for Neuroscience (Lisboa, Portugal)
2019/02/06 Human iPSC-derived neuroepithelial stem cells for neuroprotection and neuroregeneration in Machado-Joseph disease XLIX Annual meeting of the Portuguese Pharmacology Society, XXXVII Clinical Pharmacology meeting and XVIII meeting of Toxicology.
Faculty of Pharmacy of the University of Porto (Porto, Portugal)
2019/02 Mutant Ataxin-3 spreading in Machado-Joseph Disease XLIX Annual meeting of the Portuguese Pharmacology Society, XXXVII Clinical Pharmacology meeting and XVIII meeting of Toxicology.
(Porto, Portugal)
2018/10 Patient-specific iPSC-derived neuroepithelial stem cells for neuroprotection and neuroregeneration in Machado-Joseph disease 10th International Symposium on Neuroprotection and Neurorepair.
(Dresden, Germany)
2017/10 Human iPSCs-derived NESCs for cerebellar transplantation in Machado-Joseph disease 10th International Meeting of the Portuguese Society for Stem Cells and Cell Therapies
Portuguese Society for Stem Cells and Cell Therapies (Covilhã, Portugal)

Event participation

Activity description
Type of event
Event name
Institution / Organization
2022/10/11 - 2022/10/14 Participation in The 29th Annual Meeting of the European Society of Gene & Cell Therapy – ESGCT 2022
Conference
The 29th Annual Meeting of the European Society of Gene & Cell Therapy – ESGCT 2022
2021/04/05 - 2021/04/09 Advanced Course in Neuronal Circuits and Behavior
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2021/03/22 - 2021/03/26 Advanced course in Synapse structure and function
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2021/02/22 - 2021/02/26 Advanced Course on Computational Biology
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2021/02/01 - 2021/02/05 Advanced Course in High-Throughput Screening for Target and Drug Discovery
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2020/02/05 - 2020/02/07 Participation in the L Annual meeting of the Portuguese Pharmacology Society, XXXVIII Clinical Pharmacology meeting and XIX meeting of Toxicology
Meeting
L Annual meeting of the Portuguese Pharmacology Society, XXXVIII Clinical Pharmacology meeting and XIX meeting of Toxicology
2019/11/15 - 2019/11/15 Participation in the 1st Annual Meeting in Pharmaceutical Sciences
Meeting
1st Annual Meeting in Pharmaceutical Sciences.
Universidade de Coimbra Faculdade de Farmácia, Portugal
2019/05/30 - 2019/06/01 Participation in XVI Meeting of the Portuguese Society for Neuroscience XVI Meeting of the Portuguese Society for Neuroscience
2018/02/06 - 2018/02/08 Participation in the XLIX Annual meeting of the Portuguese Pharmacology Society, XXXVII Clinical Pharmacology meeting and XVIII meeting of Toxicology.
Meeting
XLIX Annual meeting of the Portuguese Pharmacology Society, XXXVII Clinical Pharmacology meeting and XVIII meeting of Toxicology
Universidade do Porto Faculdade de Farmácia, Portugal
2016/03/22 - 2016/03/22 Workshop: Doping and Ergogenic substances in sports (Substâncias Dopantes e Ergogénicas no Desporto)
Workshop
XIX National Meeting of Biology Students: From gene to patrimony (XIX Encontro Nacional de Estudantes de Biologia: Do gene ao património);
Universidade de Coimbra, Portugal
2016/03/18 - 2016/03/22 XIX National Meeting of Biology Students: From gene to patrimony (XIX Encontro Nacional de Estudantes de Biologia: Do gene ao património);
Meeting
XIX National Meeting of Biology Students: From gene to patrimony (XIX Encontro Nacional de Estudantes de Biologia: Do gene ao património);
Universidade de Coimbra, Portugal
2015/03/30 - 2015/03/30 Workshop: A New Perspective of the Bioentrepreneur - Entrepreneurs.
Workshop
XVIII National Meeting of Students of Biology: The Four Stations of Life (XVIII Encontro Nacional de Estudantes de Biologia: As Quatro Estações da Vida;)
Universidade do Minho, Portugal
2015/03/27 - 2015/03/30 XVIII National Meeting of Students of Biology: The Four Stations of Life (XVIII Encontro Nacional de Estudantes de Biologia: As Quatro Estações da Vida;)
Meeting
XVIII National Meeting of Students of Biology: The Four Stations of Life (XVIII Encontro Nacional de Estudantes de Biologia: As Quatro Estações da Vida;)
Universidade do Minho, Portugal
2014/05/15 - 2014/05/15 Workshop: Initiation to Canoeing (Iniciação à Canoagem)
Workshop
XVII National Meeting of Biology Students: Biology, a sea of knowledge (XVII Encontro Nacional de Estudantes de Biologia: Biologia, um mar de conhecimento)
Universidade do Algarve, Portugal
2014/05/12 - 2014/05/15 XVII National Meeting of Biology Students: Biology, a sea of knowledge (XVII Encontro Nacional de Estudantes de Biologia: Biologia, um mar de conhecimento)
Meeting
XVII National Meeting of Biology Students: Biology, a sea of knowledge (XVII Encontro Nacional de Estudantes de Biologia: Biologia, um mar de conhecimento);
Universidade do Algarve, Portugal

Association member

Society Organization name Role
2022 - Current European Society of Gene and Cell Therapy
2019 - Current Portuguese Society for Neurosciences Member
2015/06/03 - 2016/06/07 Núcleo de estudantes de Biologia da Associação Académica de Coimbra Colaborador no Pelouro de Desporto e lazer
2014/05/31 - 2015/06/03 Núcleo de estudantes de Biologia da Associação Académica de Coimbra Coordenador do Pelouro de Desporto
2013/06/13 - 2014/05/31 Núcleo de estudantes de Biologia da Associação Académica de Coimbra Colaborador no Pelouro de Desporto

Consulting

Activity description Institution / Organization
2018/10/10 - 2018/10/10 Scientific consultant on the development of the short movie "Machado-Joseph Disease - from the Lab to the Society" produced by the Science communication department of the Center for Neuroscience and Cell Biology, in the scope of the MSc project of Rita Amado Dias; published in youtube: https://www.youtube.com/watch?v=2ADmun6eJy0 Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Distinctions

Award

2021 SPN2021 National Scholarships (PhD Student)
Sociedade Portuguesa de Neurociências, Portugal