Ana Costa Xavier de Carvalho (3B1E-65E6-F6B9)

Curriculum Vitae

Ana Costa Xavier de Carvalho

Ana Costa Xavier de Carvalho is a group leader at the i3S in Porto and her research team is interested in studying the actomyosin dynamics that lead to network contractility in vivo. Ana completed her Licenciatura in Biochemistry at the Faculty of Sciences of the University of Porto in 1998. She was a recipient of a fellowship from the Doctoral Program G.A.B.B.A., and her doctoral studies were primarily conducted in the laboratory of Prof. William Earnshaw at the Welcome Trust Center for Cell Biology in Edinburgh, UK, where she studied the process of mitosis. Her postdoctoral work was done in the laboratory of Prof. Karen Oegema at the Ludwig Institute for Cancer Research in San Diego, USA, where she started studying the mechanisms of cytokinesis using the nematode C. elegans as model system. In 2012, Ana started her career as an independent scientist at the Institute for Cell and Molecular Biology, IBMC in Porto, Portugal. Through 3 consecutive competitive calls, Ana was awarded a contract as investigator through the Welcome II Programme in 2012 (co-funded by the Marie Curie Action Cofund and FCT, the Portuguese Foundation for Science and Technology), an investigator 5-year contract in 2014 awarded by FCT, and a 6-year contract as Principal Investigator under the FCT CEEC program (Competition for Individual Scientific Employment) that started in 2019. In March 2025, she starts an open-end contract with i3S. The work in Ana's group has been funded by an ERC Starting Grant from the European Research Council and by FCT. In her group at IBMC (which in the meantime became part of a large consortium, the i3S) her team uses live cell microscopy, genome engineering, genetics and biochemistry to perform structure-function studies using C. elegans as model organism. Their research has been mainly focused on investigating the molecular mechanisms through which an actomyosin contractile ring pinches a mother cell into two daughter cells during cytokinesis. More recently, the team started applying their knowledge and expertise to study other actomyosin networks in vivo in the context of multicellular tissues, like the C. elegans spermatheca, vulva and skeletal muscle.

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Identification

Personal identification

Full name: Ana Costa Xavier de Carvalho

Author identifiers

Ciência ID: 3B1E-65E6-F6B9
ORCID iD: 0000-0002-2256-7134

Email addresses

anacarvalho@ibmc.up.pt (Professional)

Telephones

Telephone

220408800 (Professional)

Education

	Degree	Classification
2005 Concluded	PhD (Doutoramento) Universidade do Porto Faculdade de Medicina, Portugal "Cellular and molecular analysis of survivin and the involvement of chromosomal passengers in the multinucleation phenotype of Hodgkin´s Disease" (THESIS/DISSERTATION)	n/a
1998 Concluded	Bioquimica (Licenciatura) Universidade do Porto Faculdade de Ciências, Portugal "Production and characterization of transthyretin mutants" (THESIS/DISSERTATION)	16

Affiliation

Science

	Category Host institution	Employer
2025/03/01 - Current	Principal Investigator (Research)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019/03/01 - 2025/02/28	Principal Investigator (Research)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2014/01/01 - 2018/12/31	Principal Investigator (Research)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2012/01/01 - 2013/12/31	Principal Investigator (Research)	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2005/06/01 - 2011/12/31	Postdoc (Research)	Ludwig Institute for Cancer Research San Diego Branch, United States
	Ludwig Institute for Cancer Research San Diego Branch, United States
2000/11/01 - 2005/05/31	Researcher (Research)	Wellcome Centre for Cell Biology, United Kingdom
	Wellcome Centre for Cell Biology, United Kingdom (...)
1999/11/01 - 2000/10/31	Researcher (Research)	Fundação para a Ciência e a Tecnologia, Portugal
	Universidade do Porto, Portugal

Projects

Grant

	Designation	Funders
2015/07 - 2022/06	ERC Starting grant - Mechanisms of Actomyosin-based Contractility during Cytokinesis 640553 Principal investigator	European Research Council Ongoing
2013/07 - 2015/09	Actin and myosin II in the contractile ring - mechanisms of constriction grant.Provided by PTCRIS: 125211 PTDC/BEX-BCM/0654/2012 PTDC/BEX-BCM/0654/2012 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Contract

	Designation	Funders
2019/03 - 2025/02	Mechanisms of Actomyosin-based contractility CEECIND/01967/2017	N/A
2022/01/01 - 2023/12/31	Interações entre cross-linkers de actina no sarcómero e impacto na doença muscular EXPL/BIA-CEL/1050/2021 10.54499/EXPL/BIA-CEL/1050/2021 Supervisor Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2018/07/01 - 2021/06/30	Mecanismos moleculares da maquinaria de transporte de dineína-dinactina na saúde e na doença PTDC/BIA-CEL/30507/2017 Co-Principal Investigator (Co-PI) Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2014/01/01 - 2018/12/31	The Contractile Ring During Cytokinesis: Structural Organization and Mechanisms of Constriction IF/00901/2013/CP1157/CT0001 Principal investigator Fundação para a Ciência e a Tecnologia, Portugal	Concluded
2012/01/01 - 2013/12/31	Structural and functional analysis of the contractile ring in cytokinesis DFRH/WIIA/3/2010 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions Fundação para a Ciência e a Tecnologia Concluded

Outputs

Publications

Book	Thieleke-Matos, C.; Osório, D.S.; Carvalho, A.X.; Morais-de-Sá, E.. Emerging Mechanisms and Roles for Asymmetric Cytokinesis. 2017. 10.1016/bs.ircmb.2017.01.004
Book chapter	M. I. A. Barbosa; J. Belinha; R. M. Natal Jorge; A. Carvalho. "Simulation of Cell Proliferation Using a Meshless Tool". 2023. 10.1007/978-3-031-10015-4_11 M. I. A. Barbosa; J. Belinha; R. M. Natal Jorge; A. X. Carvalho. "Simulation of Cell Proliferation Using a Particle Method". 2023. 10.1007/978-3-031-47790-4_35 M. I. A. Barbosa; J. Belinha; R. M. Natal Jorge; A. X. Carvalho. "The Effect of Oxygen and Glucose in the Cell Proliferation Process Analysed with a Meshless Method". 2023. 10.1007/978-3-031-47790-4_41 Fung Yi Chan; Ana M. Silva; Carvalho, Ana or Carvalho, AX or Ana Xavier Carvalho. "Using the Four-Cell C. elegans Embryo to Study Contractile Ring Dynamics During Cytokinesis". 2020. 10.1007/978-1-0716-0219-5_18
Journal article	Cátia Carvalho; Daniel J Barbosa; Ricardo Celestino; Esther Zanin; Carvalho, Ana or Carvalho, AX or Ana Xavier Carvalho; Reto Gassmann. Corresponding author: Reto Gassmann. "Dynein directs prophase centrosome migration to control the stem cell division axis in the developing Caenorhabditis elegans epidermis". Genetics iyae005 (2024): https://pubmed.ncbi.nlm.nih.gov/38213110/. Open access • In press • 10.1093/genetics/iyae005 Lebedev, Mikhail; Chan, Fung-Yi; Lochner, Anna; Bellessem, Jennifer; Osório, Daniel S.; Rackles, Elisabeth; Mikeladze-Dvali, Tamara; Carvalho, Ana Xavier; Zanin, Esther. "Anillin forms linear structures and facilitates furrow ingression after septin and formin depletion". Cell Reports 42 9 (2023): 113076. http://dx.doi.org/10.1016/j.celrep.2023.113076. 10.1016/j.celrep.2023.113076 M. I. A. Barbosa; J. Belinha; R. M. Natal Jorge; A. X. Carvalho. "Computationally modelling cell proliferation: A review". International Journal for Numerical Methods in Biomedical Engineering (2023): https://doi.org/10.1002/cnm.3715. 10.1002/cnm.3715 Inês C. Santos; Ana M. Silva; Reto Gassmann; Ana X. Carvalho. "Anillin and the microtubule bundler PRC1 maintain myosin in the contractile ring to ensure completion of cytokinesis". Development (2023): https://doi.org/10.1242/dev.201637. 10.1242/dev.201637 Trevor J. Barker; Fung-Yi Chan; Carvalho, Ana or Carvalho, AX or Ana Xavier Carvalho; Meera V. Sundaram. Corresponding author: Meera V. Sundaram. "Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva". microPublication Biology (2023): https://www.micropublication.org/journals/biology/micropub-biology-000863. Open access • Accepted Helder Rocha; Patrícia A. Simões; Jacqueline Budrewicz; Pablo Lara-Gonzalez; Ana Xavier Carvalho; Julien Dumont; Arshad Desai; Reto Gassmann. "Nuclear-enriched protein phosphatase 4 ensures outer kinetochore assembly prior to nuclear dissolution". Journal of Cell Biology (2023): https://doi.org/10.1083/jcb.202208154. 10.1083/jcb.202208154 Ana Marta Silva; Fung-Yi Chan; Michael J. Norman; Ana Filipa Sobral; Esther Zanin; Reto Gassmann; Julio Monti Belmonte; Ana Xavier Carvalho. "ß-heavy-spectrin stabilizes the constricting contractile ring during cytokinesis". Journal of Cell Biology (2023): https://doi.org/10.1083/jcb.202202024. 10.1083/jcb.202202024 Ricardo Celestino; José B. Gama; Artur F. Castro-Rodrigues; Daniel J. Barbosa; Helder Rocha; Ennio A. d’Amico; Andrea Musacchio; et al. "JIP3 interacts with dynein and kinesin-1 to regulate bidirectional organelle transport". Journal of Cell Biology (2022): https://doi.org/10.1083/jcb.202110057. 10.1083/jcb.202110057 Ana R.G. De-Castro; Diogo R.M. Rodrigues; Maria J.G. De-Castro; Neide Vieira; Cármen Vieira; Ana X. Carvalho; Reto Gassmann; Carla M.C. Abreu; Tiago J. Dantas. "WDR60-mediated dynein-2 loading into cilia powers retrograde IFT and transition zone crossing". Journal of Cell Biology (2022): https://doi.org/10.1083/jcb.202010178. 10.1083/jcb.202010178 Sobral, Ana Filipa; Chan, Fung-Yi; Norman, Michael J.; Osório, Daniel S.; Dias, Ana Beatriz; Ferreira, Vanessa; Barbosa, Daniel J.; et al. "Plastin and spectrin cooperate to stabilize the actomyosin cortex during cytokinesis". Current Biology 31 24 (2021): 5415-5428.e10. http://dx.doi.org/10.1016/j.cub.2021.09.055. 10.1016/j.cub.2021.09.055 Daniel José Barbosa; Joana Duro; Carvalho, Ana or Carvalho, AX or Ana Xavier Carvalho; Reto Gassmann. "Dynein-dynactin segregate meiotic chromosomes in C. elegans spermatocytes". Development 148 3 (2021): https://dev.biologists.org/content/148/3/dev197780.long. Open access • Accepted • doi: 10.1242/dev.197780. Joana Leite; Chan, Fung-Yi; Daniel Sampaio Osorio; Joana Saramago; Ana Filipa Sobral; Ana Marta Silva; Reto Gassmann; Carvalho, Ana or Carvalho, AX or Ana Xavier Carvalho. "Equatorial Non-muscle Myosin II and Plastin Cooperate to Align and Compact F-actin Bundles in the Cytokinetic Ring". Frontiers in Cell and Developmental Biology (2020): https://www.frontiersin.org/articles/10.3389/fcell.2020.573393/full. 10.3389/fcell.2020.573393 Hai Wei; Eric J Lambie; Daniel Sampaio Osório; Carvalho, Ana or Carvalho, AX or Ana Xavier Carvalho; Barbara Conradt. "PIG-1 MELK-dependent phosphorylation of nonmuscle myosin II promotes apoptosis through CES-1 Snail partitioning". PLoS Genetics (2020): https://pubmed.ncbi.nlm.nih.gov/32946434/. 10.1371/journal.pgen.1008912 Daniel S. Osório; Fung Yi Chan; Joana Saramago; Joana Leite; Ana Marta Silva; Ana F Sobral; Reto Gassmann; Carvalho, Ana or Carvalho, AX or Ana Xavier Carvalho. "Crosslinking activity of non-muscle myosin II is not sufficient for embryonic cytokinesis in C. elegans". Development (2019): http://dx.doi.org/10.1242/dev.179150. 10.1242/dev.179150 Joana Leite; Daniel Sampaio Osorio; Ana Filipa Sobral; Carvalho, Ana or Carvalho, AX or Ana Xavier Carvalho. "Network Contractility during Cytokinesis - From Molecular to Global Views". Biomolecules (2019): http://dx.doi.org/10.3390/biom9050194. 10.3390/biom9050194 Celestino R; Henen MA; Gama JB; Carvalho C; McCabe M; Barbosa DJ; Born A; et al. "A transient helix in the disordered region of dynein light intermediate chain links the motor to structurally diverse adaptors for cargo transport.". PLoS biology (2019): http://europepmc.org/abstract/med/30615611. 10.1371/journal.pbio.3000100 Chan FY; Silva AM; Saramago J; Pereira-Sousa J; Brighton HE; Pereira M; Oegema K; Gassmann R; Carvalho AX. "The ARP2/3 complex prevents excessive formin activity during cytokinesis.". Molecular biology of the cell (2018): http://europepmc.org/abstract/med/30403552. 10.1091/mbc.e18-07-0471 Pereira C; Reis RM; Gama JB; Celestino R; Cheerambathur DK; Carvalho AX; Gassmann R. "Self-Assembly of the RZZ Complex into Filaments Drives Kinetochore Expansion in the Absence of Microtubule Attachment.". Current biology : CB (2018): http://europepmc.org/abstract/med/30415699. 10.1016/j.cub.2018.08.056 Sriyash Mangal; Jennifer Sacher; Taekyung Kim; Daniel Sampaio Osório; Fumio Motegi; Ana Xavier Carvalho; Karen Oegema; Esther Zanin. "TPXL-1 activates Aurora A to clear contractile ring components from the polar cortex during cytokinesis". The Journal of Cell Biology 217 3 (2018): 837-848. https://doi.org/10.1083/jcb.201706021. 10.1083/jcb.201706021 Simões PA; Celestino R; Carvalho AX; Gassmann R. "NudE regulates dynein at kinetochores but is dispensable for other dynein functions in the C. elegans early embryo.". Journal of cell science (2018): http://europepmc.org/abstract/med/29192061. 10.1242/jcs.212159 José B. Gama; Cláudia Pereira; Patrícia A. Simões; Ricardo Celestino; Rita M. Reis; Daniel J. Barbosa; Helena R. Pires; et al. "Molecular mechanism of dynein recruitment to kinetochores by the Rod–Zw10–Zwilch complex and Spindly". The Journal of Cell Biology 216 4 (2017): 943-960. https://doi.org/10.1083/jcb.201610108. 10.1083/jcb.201610108 Vieira, N.; Bessa, C.; Rodrigues, A.J.; Marques, P.; Chan, F.-Y.; de Carvalho, A.X.; Correia-Neves, M.; Sousa, N.. "Sorting nexin 3 mutation impairs development and neuronal function in Caenorhabditis elegans". Cellular and Molecular Life Sciences (2017): 1-18. http://www.scopus.com/inward/record.url?eid=2-s2.0-85035812768&partnerID=MN8TOARS. 10.1007/s00018-017-2719-2 Barbosa, D.J.; Duro, J.; Prevo, B.; Cheerambathur, D.K.; Carvalho, A.X.; Gassmann, R.. "Dynactin binding to tyrosinated microtubules promotes centrosome centration in C. elegans by enhancing dynein-mediated organelle transport". PLoS Genetics 13 7 (2017): http://www.scopus.com/inward/record.url?eid=2-s2.0-85026624927&partnerID=MN8TOARS. 10.1371/journal.pgen.1006941 Ana M. Silva; Daniel S. Osório; Antonio J. Pereira; Helder Maiato; Inês Mendes Pinto; Boris Rubinstein; Reto Gassmann; Ivo Andreas Telley; Ana Xavier Carvalho. "Robust gap repair in the contractile ring ensures timely completion of cytokinesis". The Journal of Cell Biology 215 6 (2016): 789-799. https://doi.org/10.1083/jcb.201605080. 10.1083/jcb.201605080 De Carvalho, A.X.; Maiato, H.; Maia, A.F.; Ribeiro, S.A.; Pontes, P.; Bickmore, W.; Earnshaw, W.C.; Sambade, C.. "Reed-sternberg cells form by abscission failure in the presence of functional aurora B kinase". PLoS ONE 10 5 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84929096316&partnerID=MN8TOARS. 10.1371/journal.pone.0124629 Davies, T.; Jordan, S.N.; Chand, V.; Sees, J.A.; Laband, K.; Carvalho, A.X.; Shirasu-Hiza, M.; et al. "High-Resolution Temporal Analysis Reveals a Functional Timeline for the Molecular Regulation of Cytokinesis". Developmental Cell 30 2 (2014): 209-223. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904886021&partnerID=MN8TOARS. 10.1016/j.devcel.2014.05.009 Carvalho, A.; Olson, S.K.; Gutierrez, E.; Zhang, K.; Noble, L.B.; Zanin, E.; Desai, A.; Groisman, A.; Oegema, K.. "Acute drug treatment in the early C. elegans embryo". PLoS ONE 6 9 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-80052849441&partnerID=MN8TOARS. 10.1371/journal.pone.0024656 Zanin, E.; Dumont, J.; Gassmann, R.; Cheeseman, I.; Maddox, P.; Bahmanyar, S.; Carvalho, A.; et al. "Affinity Purification of Protein Complexes in C. elegans". Methods in Cell Biology 106 (2011): 289-322. http://www.scopus.com/inward/record.url?eid=2-s2.0-82355184610&partnerID=MN8TOARS. 10.1016/B978-0-12-544172-8.00011-6 Lewellyn, L.; Carvalho, A.; Desai, A.; Maddox, A.S.; Oegema, K.. "The chromosomal passenger complex and centralspindlin independently contribute to contractile ring assembly". Journal of Cell Biology 193 1 (2011): 155-169. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955513613&partnerID=MN8TOARS. 10.1083/jcb.201008138 Fant, X.; Samejima, K.; Carvalho, A.; Ogawa, H.; Xu, Z.; Yue, Z.; Earnshaw, W.C.; Ruchaud, S.. "Use of DT40 conditional-knockout cell lines to study chromosomal passenger protein function". Biochemical Society Transactions 38 6 (2010): 1655-1659. http://www.scopus.com/inward/record.url?eid=2-s2.0-78649741327&partnerID=MN8TOARS. 10.1042/BST0381655 Carvalho, A.; Desai, A.; Oegema, K.. "Structural Memory in the Contractile Ring Makes the Duration of Cytokinesis Independent of Cell Size". Cell 137 5 (2009): 926-937. http://www.scopus.com/inward/record.url?eid=2-s2.0-65849336045&partnerID=MN8TOARS. 10.1016/j.cell.2009.03.021 Yue, Z.; Carvalho, A.; Xu, Z.; Yuan, X.; Cardinale, S.; Ribeiro, S.; Lai, F.; et al. "Deconstructing Survivin: Comprehensive genetic analysis of Survivin function by conditional knockout in a vertebrate cell line". Journal of Cell Biology 183 2 (2008): 279-296. http://www.scopus.com/inward/record.url?eid=2-s2.0-55949106979&partnerID=MN8TOARS. 10.1083/jcb.200806118 Gassmann, R.; Kline, S.L.; Carvalho, A.; Desai, A.. "Analysis of kinetochore assembly and function in Caenorhabditis elegans embryos and human cells". Methods 41 2 (2007): 177-189. http://www.scopus.com/inward/record.url?eid=2-s2.0-33845781985&partnerID=MN8TOARS. 10.1016/j.ymeth.2006.07.027 Noton, E.A.; Colnaghi, R.; Tate, S.; Starck, C.; Carvalho, A.; Ferrigno, P.K.; Wheatley, S.P.. "Molecular analysis of survivin isoforms: Evidence that alternatively spliced variants do not play a role in mitosis". Journal of Biological Chemistry 281 2 (2006): 1286-1295. http://www.scopus.com/inward/record.url?eid=2-s2.0-33644854070&partnerID=MN8TOARS. 10.1074/jbc.M508773200 Gassmann, R.; Carvalho, A.; Henzing, A.J.; Ruchaud, S.; Hudson, D.F.; Honda, R.; Nigg, E.A.; Gerloff, D.L.; Earnshaw, W.C.. "Borealin: A novel chromosomal passenger required for stability of the bipolar mitotic spindle". Journal of Cell Biology 166 2 (2004): 179-191. http://www.scopus.com/inward/record.url?eid=2-s2.0-3342887700&partnerID=MN8TOARS. 10.1083/jcb.200404001 Carvalho, A.; Carmena, M.; Sambade, C.; Earnshaw, W.C.; Wheatley, S.P.. "Survivin is required for stable checkpoint activation in taxol-treated HeLa cells". Journal of Cell Science 116 14 (2003): 2987-2998. http://www.scopus.com/inward/record.url?eid=2-s2.0-0042170180&partnerID=MN8TOARS. 10.1242/jcs.00612 Wheatley, S.P.; Carvalho, A.; Vagnarelli, P.; Earnshaw, W.C.. "INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis". Current Biology 11 11 (2001): 886-890. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035810919&partnerID=MN8TOARS. 10.1016/S0960-9822(01)00238-X
Preprint	Vanessa Teixeira; Kashish Singh; José B. Gama; Ricardo Celestino; Ana Xavier Carvalho; Paulo Pereira; Carla M.C. Abreu; et al. "CDR2 is a dynein adaptor recruited by kinectin to regulate ER sheet organization". 2024. https://doi.org/10.1101/2024.11.06.622207. 10.1101/2024.11.06.622207 Cátia Carvalho; Daniel J. Barbosa; Ricardo Celestino; Esther Zanin; Ana Xavier Carvalho; Reto Gassmann. "Dynein directs prophase centrosome migration to control the stem cell division axis in the developing C. elegans epidermis". 2023. https://doi.org/10.1101/2023.09.25.559295. 10.1101/2023.09.25.559295 Helder Rocha; Patrícia A. Simões; Jacqueline Budrewicz; Pablo Lara-Gonzalez; Ana Xavier Carvalho; Julien Dumont; Arshad Desai; Reto Gassmann. "Nuclear-enriched protein phosphatase 4 ensures outer kinetochore assembly prior to nuclear dissolution". 2022. https://doi.org/10.1101/2022.09.01.505886. 10.1101/2022.09.01.505886 Mikhail Lebedev; Fung-Yi Chan; Jennifer Bellessem; Daniel S. Osório; Elisabeth Rackles; Tamara Mikeladze-Dvali; Ana X. Carvalho; Esther Zanin. "Anillin forms linear structures and facilitates furrow ingression after septin and formin depletion". 2022. https://doi.org/10.1101/2022.12.22.521621. 10.1101/2022.12.22.521621

Other

Other output

JIP3 regulates bi-directional organelle transport in neurons through its interaction with dynein and kinesin-1. preprint bioRxiv. 2021. Ricardo Celestino; José B. Gama; Artur F. Castro-Rodrigues; Barbosa, Daniel J.; Ennio A. d’Amico; Andrea Musacchio; Carvalho, Ana or Carvalho, AX or Ana Xavier Carvalho; Gassmann, Reto. https://doi.org/10.1101/2021.10.11.463801.
Src-dependent NM2A tyrosine-phosphorylation regulates actomyosin dynamics (preprint in bioRxiv). Preprint in bioRxiv. 2020. Cláudia Brito; Daniel S. Osório; Joana Pereira; Neil Billington; James R. Sellers; Didier Cabanes; Ana Xavier Carvalho (3B1E-65E6-F6B9); Sandra Sousa. https://doi.org/10.1101/2020.08.12.246090.
https://doi.org/10.1101/2020.08.12.246090
Flow-independent accumulation of motor-competent non-muscle myosin II in the contractile ring is essential for cytokinesis. 2018. Daniel Sampaio Osorio; Fung Yi Chan; Joana Saramago; Joana Leite; Ana Marta Silva; Ana Filipa Sobral; Reto Gassmann; Ana Xavier Carvalho. https://doi.org/10.1101/333286.
10.1101/333286
Robust Gap Repair in the Contractile Ring Ensures Timely Completion of Cytokinesis. 2016. Silva AM; Osorio DS; Pereira AJ; Maiato H; Mendes Pinto IM; Rubinstein B; Gassmann R; Telley IAA; Carvalho AXd. http://europepmc.org/abstract/PPR/PPR29603.
10.1101/052704

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2025/01/27	C. elegans as a versatile platform for toxicity and biocompatibility assays	Mind the Gap: In vivo non-mammal models for biocompatibility/toxicity testing i3s - Instituto de Investigação e Inovação em Saúde, Universidade do Porto (Porto, Portugal)
2024/12/10	Worming our way to discovery: The power of C. elegans in modern science	Message in a bottle: the world of RNAs i3s - Instituto de Investigação e Inovação em Saúde, Universidade do Porto (Porto, Portugal)
2024/11/20	C. elegans as a unique experimental model organism - Modelos Biológicos Mestrado em Aplicações de Biotecnologia e Biologia Sintética, FCUP, Porto, Portugal	Modelos Biológicos Mestrado em Aplicações de Biotecnologia e Biologia Sintética, FCUP, Porto, Portugal FCUP, Porto, Portugal (Porto, Portugal)
2024/10/18	C. elegans: A powerful model for advancing the 3Rs in biomedical research	3Rs Day – Models in Biomedical Research i3s - Instituto de Investigação e Inovação em Saúde, Universidade do Porto (Porto, Portugal)
2024/09/25	Finding the best format to foster interactions between researchers and clinicians	NeuroDay i3s - Instituto de Investigação e Inovação em Saúde, Universidade do Porto (Porto, Portugal)
2024/01/23	Functional diversity of actin crosslinkers and myosin motors	Institute of Molecular Biology seminar series, University of Oregon Institute of Molecular Biology, University of Oregon (United States)
2023/11	C. elegans, an unique experimental model organism	Modelos Biológicos Mestrado em Aplicações de Biotecnologia e Biologia Sintética FCUP, Porto, Portugal
2023/06/19	Linked-in - the roles of actin crosslinkers during cytokinesis	VIP & DIF Institut Jacques Monod (Paris, France)
2023/01/30	Actin filament network connectivity during cytokinesis	External Invited Seminars IGBMC, IGBMC - Institute de Génétique et de Biologie Moleculaire et Cellulaire, Strasbourg, France (Strasbourg, France)
2022/10/26	C. elegans – a unique experimental model organism	Modelos Biológicos Mestrado em Aplicações de Biotecnologia e Biologia Sintética FCUP (Porto, Portugal)
2022/07/30	Spectrins stabilize the contractile ring during cytokinesis	European Worm Meeting 2022 University of Vienna (Vienna, Austria)
2021/11/17	C. elegans a unique experimental model organism	Mestrado em Aplicações de Biotecnologia e Biologia Sintética FCUP (Portugal)
2021/11/04	Presenting our research to schools visiting i3S	Escolas i3S i3S (Portugal)
2021/02/23	Cooperation between actin filament crosslinkers during cytokinesis	Quantitative and Computational Developmental Biology Cluster North Caroline State University (Raleigh, United States)
2020/12/15	Cooperation between actin filament crosslinkers during cytokinesis	ASCB/EMBO meeting 2020 ASCB/EMBO meeting 2020 (United States)
2020/06/22	New insights into the roles of actin filament crosslinkers during cytokinesis	2020 European C. elegans Meeting (France)
2019/10/30	New insights into actin filament crosslinking during cytokinesis	Cancer Program Seminar Series, i3S i3S, University of Porto, Portugal (Porto, Portugal)
2019/07	C. elegans as a model organism	Model Organisms in Human Disease The right organism for a specific question FFUP/ICBAS, University of Porto (Porto, Portugal)
2019/03/22	GENiE - Probing the contribution of the cell cortex to ring constriction during cytokinesis	5th GENIE MEETING COST Action BM1408 (Kandersteg, Switzerland)
2019/02/07	Worm Cytoskeleton: Probing the contribution of the cell cortex to ring constriction during cytokinesis	Worm Cytoskeleton Workshop COST Action BM1408, Tel Aviv University (Tel Aviv, Israel)
2018/10/25	How cells use their actin cytoskeleton to divide	IV Symposium PDBMA CBMA - Universidade do Minho (Braga, Portugal)
2018/06/21	Exploring myosin's roles during cytokinesis	6th International Iberian Biophysics Congree, X Iberoamerican Congress of Biophysics IIBC (Castellon, Spain)
2018/03/26	Actomyosin Dynamics during Cytokinesis	Seminar Series MRC Laboratory for Molecular Cell Biology (London, United Kingdom)
2017/06/01	Forces in Cytokinesis	Symposium Forces in Biology i3S (Porto, Portugal)
2017/05/08	Functional Dissection of Branched and Non-branched actin filament networks during cytokinesis	Actin and microtubule cytoskeleton: bridging scales from single molecules to tissues CNRS - Conférences Jacques Monod (Roscoff)
2016/09/07	Robust Gap Repair in the Contractile Ring Ensures Timely Completion of Cytokinesis	Actin in Action: From Molecules to Cellular Functions EMBL Heidelberg, Germany (Heidelberg, Germany)
2016/07/15	How to apply for an ERC grant	SESSIONS @ COFFEE LOUNGE FEUP (porto, Portugal)
2016/04/07	Acto-myosin dynamics during cytokinesis	Cancer Program Seminar Series i3S (Porto, Portugal)
2015/06/29	Mechanics of the contractile ring during cytokinesis	Seminar Series IMM (porto, Portugal)
2015/06/29	Mechanics of the contractile ring during cytokinesis	Monday Lecture - External Seminar Series Instituto de Medicina Moleculae (Lisbon, Portugal)
2015/06	Mechanics of the contractile ring during cytokinesis	Building Bridges in Quantitative Biology IBMC and Mathematics Department University of Porto
2015/02/26	Laser microsurgery in the contractile ring	IEEE 4th Portuguese Meeting on Bioengineering FEUP (Porto, Portugal)
2014/10/30	Actin filament network remodeling during cytokinesis	i3S 4th Annual Meeting i3S (Porto, Portugal)
2013/07/17	Science heartbeat	GABBA Annual Symposium GABBA Doctoral Program, University of Porto (Porto, Portugal)
2012/06/18	Live cell imaging assays to study cytokinesis in the early C. elegans embryo	4th Course on Optical Microscopy Imaging for Biosciences IBMC (Porto, Portugal)
2012/05/10	xxxxxxx	Third I3S Scientific Retreat Abstract Book IBMC (Povoa do Varzim, Portugal)
2011	xxxxxxx	Invited seminars Instituto Gulbenkian Ciencia (Porto, Portugal)
2011	xxxxx	Invited Seminars IBMC (Porto, Portugal)
2011	xxxxxxx	Invited Seminars Max F. Perutz laboratories, Austria (Vienna, Austria)
2009/06/25	Cytokinesis in C. elegans: Structural Memory in the Contractile Ring	Invited Seminars Instituto Gulbenkian Ciência (Oeiras, Portugal)
2009/06/21	Cytokinesis in C. elegans: Structural Memory in the Contractile Ring	Invited Seminars IBMC (Porto, Portugal)
2009/05/15	Structural Memory in the Contractile Ring Makes the Duration of Cytokinesis Independent of Cell Size	Seminars University of North Carolina (Chapel Hill, United States)
2008/04/16	Dynamics of the contractile ring filament network during cytokinesis	American Society for Biochemistry and Molecular Biology meeting American Society for Biochemistry and Molecular Biology (San Diego, United States)
2007/06	Dynamics of the contractile ring filament network during cytokinesis	FASEB Meeting FASEB (Indian Wells, United States)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2024/01/01 - 2027/12/31	Dissecting the roles of Anillin in tissue morphogenesis Supervisor	Ciências Biomédicas (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2023/09/20 - 2025/02/28	Investigating Formin's Contribution to Abscission Supervisor	Master in Cellular and Molecular Biology (Master) Universidade de Coimbra, Portugal
2018 - 2024/10/02	Biomechanics of cytokinesis: studying the interplay between the contractile ring and the cell cortex Supervisor	Ciencias Biomedicas (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2024/01 - 2024/09	Developing fluorescent reporters of the actin cytoskeleton and plasma membrane in the C. elegans skeletal muscle Supervisor	Estágio Biologia (Scientific initiation) Universidade do Porto Faculdade de Ciências, Portugal
2019 - 2024/07/29	Development of meshless and particle methods based non-linear algorithms to numerically simulate cellular proliferation and tumour growth Co-supervisor	Engenharia Mecânica (PhD) Universidade do Porto Faculdade de Engenharia, Portugal
2017/09/01 - 2023/09/28	Dissection of molecular mechanisms involved in the last stages of cytokinesis Supervisor	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2022 - 2023	Synergies between actin crosslinkers in muscle in vivo Supervisor	Biochemistry (Degree) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021 - 2022	Interactions between Sarcomere Actin Crosslinkers and Impact on Muscle Disease Supervisor	Bioquimica (Degree) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2016/09/01 - 2021/12/31	Functionalities and interplays of ActinFilament Crosslinkers in vivo Supervisor	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2015/11/27 - 2021/04/08	Novel views on non-muscle actomyosin networks: a multi-system analysis of myosin function in the C. elegans metazoan model Supervisor	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2019/09/15 - 2020/12/22	Actomyosin regulation in the germline versus somatic gonad in C. elegans - Bioquímica em Saúde Co-supervisor	Bioquímica em Saúde (Master) Instituto Politécnico do Porto, Portugal Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019/09/01 - 2020/02/01	Actomyosin regulation in the germline versus somatic gonad Caenorhabditis elegans - Biology and Biochemistry Supervisor	Biology and Biochemistry (Other) Università degli Studi di Modena e Reggio Emilia, Italy Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2018/09/17 - 2019/12/17	Dissecting formin's molecular mechanisms of action during embryonic cell division Supervisor	Universidade do Porto Faculdade de Ciências, Portugal Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2016/09/01 - 2018/11	Unveil the role of actin on KRAS-induced autophagy in colorectal cancer, using Caenorhabditis elegans as an in vivo model Supervisor	Universidade do Minho, Portugal Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2013 - 2017	Aiptasia sp. larva as a model to study the cellular mechanisms of coral-algae symbiosis Co-supervisor	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2013 - 2017	Discovering Pathways underlying autism spectrum disorder upon loss-of-function of chromatin-related genes Co-supervisor	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2012/09/06 - 2013/12/18	Phospho-regulation of myosin regulatory chain in Caenorhabditis elegans embryos during cytokinesis Supervisor	Universidade do Porto Faculdade de Ciências, Portugal Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Event organisation

	Event name Type of event (Role)	Institution / Organization
2023/01/01 - Current	The conference will be on “Forces across scales” and will be held at i3S (Porto, Portugal) from March 6th to March 9th, 2024. The intention is to discuss the latest developments regarding biological forces, from single molecule studies to cell and developmental processes. (2023/01/01 - 2024/03/09) Conference (Co-organisor)
2018/09/01 - 2019/02/06	Workshop on the Worm cytoskeleton - Gathering of principal investigators in Europe studying the cell cytoskeleton using the C. elegans system (2019/02/07 - 2019/02/08) Workshop (Co-organisor)	Tel Aviv University, Israel
2017/01/01 - 2017/11/10	6th Annual Meeting i3S, annual meeting for the whole institute (>600 people) (2017/11/09 - 2017/11/10) Conference (Member of the Organising Committee)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2016/11/01 - 2017/03/16	First Retreat of the Cancer integrative program. This event aimed at gathering the most senior investigator of i3S whose research is integrated in the Cancer program of i3S. About 100 people participated. (2017/03/17 - 2017/03/17) Conference (Co-organisor)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Event participation

	Activity description Type of event	Event name Institution / Organization
2019/07/08 - Current	Symposium on model organisms used to study human disease Symposium	Model Organisms in human Disease: the right organism for a specific question Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2019/02/07 - Current	Worm Cytoskeleton Workshop Workshop	Worm Cytoskeleton
2018/10/25 - Current	IV Symposium of the Doctoral Program in in Molecular and Environmental Biology Symposium	IV Symposium of the Doctoral Program in in Molecular and Environmental Biology Universidade do Minho Centro de Biologia Molecular e Ambiental, Portugal
2018/07/18 - Current	Short-course on ERC grants	Short-course on ERC grants, i3S Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2025/01/01 - 2025/06/30	Projeto SEI - Sociedade Educação Investigação - develop a scientif project with 11 students of the 11º year, Escola Secundaria Alexandre Herculano, Porto + i3S Other	Projeto SEI Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal Câmara Municipal do Porto, Portugal
2021/12/01 - 2021/12/10	Attended seminars on cell biology, namely actin network dynamics, cytokinesis, actin regulators associated diseases Conference	Cell Bio Virtual 2021 - An Online ASCB/EMBO Meeting American Society for Cell Biology, United States
2021/09/24 - 2021/09/24	Presented the work developed in our team at i3S to the public Exhibition	Noite Europeia dos Investigadores - NEI 2021 Ciência Viva, Portugal
2019/12/08 - 2019/12/12	ASCB \| EMBO 2018 Meeting Conference	ASCB \| EMBO 2018 Meeting American Society for Cell Biology, United States
2018/06/20 - 2018/06/22	Conference by Spanish Biophysical Society, the Portuguese Biophysical Society (SPBf) and the Latin American Federation of Biophysical Societies (LAFeBS) Conference	6th International Iberian Biophysics Congress & X Iberoamerican Congress of Biophysics
2018/06/13 - 2018/06/17	EMBO - C. elegans development, cell biology and gene expression Conference	C. elegans development, cell biology and gene expression
2017/12/02 - 2017/12/06	ASCB EMBO meeting 2017 - oral presentation from our group by Fung-Yi Chan + posters by Ana Filipa Sobral and Daniel Osorio Conference	American Society for Cell Biology, United States

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2025/02/07	The impact of cell-cell and cell matrix adhesion on the efficiency of epithelial cytokinesis (Thesis) Arguer	Margarida Gonçalves (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2024	MAP7D3 Reads the Tubulin Code to Regulate Kinesin-1-mediated Cell Polarization Required for Directed Cell Migration (Thesis) Main arguer	Miguel Marques Simões da Silva (Master) Universidade do Porto Faculdade de Engenharia, Portugal Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2023/01/30	Étude des relations entre architectures d’actine, contenu moléculaire et identité cellulaire au cours de l’embryogénèse précoce de C. elegans (Thesis) Main arguer	Grégoire MATHONNET (PhD) Université de Strasbourg, France
2022	Biophysics of mitotic spindle assembly (Thesis) Arguer	Vanessa Nunes (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021/09/24	Asymmetric division in single cell nematode embryos outside the Caenorhabditis genus (Thesis) Arguer	Dureen Samandar Eweis (PhD) Université PSL, France
2021/06/15	Biochemical regulation of cell mechanics in C. elegans embryo (Thesis) Arguer	Serena Prigent Garcia (PhD) Association Sorbonne Université, France
2020/12/17	Deciphering the molecular mechanisms regulating dystroglycan-dystrophin localization during cytokinesis (Thesis) Main arguer	Carolina Ramos (Master) Universidade do Porto Faculdade de Ciências, Portugal Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2020	Host cytoskeletal responses against bacterial pore-forming toxins (Thesis) Arguer	Claudia Fernandes Brito (PhD)
2019/12/10	ProfilinI delivery tunes cytoskeleton dynamics towards CNS axon (Thesis) Main arguer	Ana Rita Pinto Costa (PhD)
2019/11/07	Force transmission and endothelial cell rearrangements during vascular remodeling (Thesis) Main arguer	Joana Cristina Reino Carvalho (PhD)
2018/10	Unveil the role of actin on KRAS induced autophagy in colorectal cancer, using Caenorhabditis elegans as an “in vivo" model Supervisor	Adriana Manuela da Silva Mendes (Master) Universidade do Minho Escola de Ciências, Portugal
2018	Mechanisms of actin cytoskeletal dynamics in oligodendrocyte biology and myelin formation (Thesis) Arguer	Maria Manuela Coutinho Alves de Azevedo (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2017	Functional Characterization of the actin-bundling protein plastin in C. elegans early embryogenesis (Thesis) Arguer	Ding Wei Yung (PhD) National University of Singapore, Singapore
2017	Discovering Pathways underlying autism spectrum disorder upon loss-of-function of chromatin-related gens Thesis Member	Catarina Morais Seabra (PhD)
2017	Aiptasia sp. as a model to study the cellular mechanisms of coral-algae symbiosis Thesis Member	Iliona Wolfowicz (PhD) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2016	Impact of aneuploidy-induced proteomic imbalances on chromosomal stability (Thesis) Arguer	Monika Barroso Vilares (Master) Universidade do Porto Faculdade de Ciências, Portugal Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Association member

	Society Organization name	Role
2002/01/01 - Current	American Society for Cell Biology	Scientific member
2023/01/01 - 2023/12/31	Genetics Society of America	Member

Committee member

	Activity description Role	Institution / Organization
2025/01/01 - Current	Organization committee of BioEngHub - monthly seminars on bioengineering Member	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2024/01/01 - Current	Committee of i3S-centro acadénico clinico CHUdSA/ICBAS to foster collaborations between i3S researchers and clinicians at Hospital Santo António (CHUdSA) Member	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal Centro Hospitalar Universitário de Santo António, Portugal Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2023/06/16 - Current	European representative of the WormBoard - committee that represents the entire worm world -wide community Member
2015/04/01 - Current	COST Action BM1408 - Portuguese representative in management committee of the COST Action BM1408 Other	European Union - COST - European Cooperation in Science and Technology, Belgium
2014 - Current	Member of the external seminar committee of IBMC - committee in charge of selecting external speakers that visit IBMC every Friday to give a scientific seminar Member	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2015 - 2017	IBMC executive committee - executive meeting of the Institute for Molecular and Cell Biology, with direction, one representative of each sector. I co-represented the senior researchers. Member	Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Conference scientific committee

	Conference name	Conference host
2023/06/24 - 2023/06/28	24th International C. elegans Conference	GSA

Course / Discipline taught

	Academic session	Degree Subject (Type)	Institution / Organization
2013/05 - 2019/05	GABBA PhD Program, Module on Cell Division	Cell Division (Doutoramento)	Universidade do Porto, Portugal

Interview (newspaper / magazine)

	Activity description	Newspaper / Forum
2020/01/13	Interview about our lab and an article we published in Development: Osorio et al., 2019 "Crosslinking activity of non-muscle myosin II is not sufficient for embryonic cytokinesis in C. elegans"	Development/The people behind the papers
2015/01/19	Pessoas da UP	Notícias Universidade do Porto
2014/12/14	Noticias Universidade do Porto - "Duas das novas "estrelas" da Ciência Europeia estão no IBMC"	Noticias Universidade do Porto

Mentoring / Tutoring

	Topic	Student name
2018 - Current	Doctoral Program in Molecular and Cell Biology, IBMC	Ana Margarida Gonçalves dos Santos
2018 - Current	Doctoral Program in Molecular and Cell Biology - IBMC	Margarida Gomes

Distinctions

Award

2019	Principal Investigator Individual Position FCT CEECIND/01967/2017 Fundação para a Ciência e a Tecnologia, Portugal
2014	FCT Individual Investigator Position IF/00901/2013/CP1157/CT0001 Fundação para a Ciência e a Tecnologia, Portugal
2011	Welcome II programme, Marie Curie Fellow Fundação para a Ciência e a Tecnologia, Portugal EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions, Belgium
2009	Kerr Award for Research Excellence Ludwig Institute for Cancer Research, United States

Other distinction

2006	Postdoctoral fellowship Fundação para a Ciência e a Tecnologia, Portugal
1999	PhD fellowship from the GABBA Doctoral Program Fundação para a Ciência e a Tecnologia, Portugal