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Johnny Lisboa obtained his Master's Degree in Biochemistry in 2010 by the University Pierre et Marie Curie (Paris, France). In 2013, he obtained his PhD on Biochemistry and Biophysics by University of Paris-Sud, under the supervision of Dr. Sophie Quevillon-Cheruel at the Institute for Integrative Biology of the Cell (I2BC, Paris). His PhD project focus in the structural and functional study of specific proteins involved in the natural transformation systems of various human pathogens, in order to understand their molecular mechanisms and interaction networks. Using X-ray crystallography and NMR as the main technical approach, complemented by a plethora of other biochemical, biophysical, and computational techniques, he has been studying the function of macromolecules and macromolecular complexes from their high-resolution structures. He also used powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. In 2014, he joined the Fish Immunology and Vaccinology (FIV) Group at i3S (Porto, Portugal) as postdoc, to apply his expertise to the microbiology field. Since then, he has been focused on the structural and functional characterization of AB toxins, mainly AIP56 and NiTox, key virulence factors secreted by Photobacterium damselae subsp. piscicida (Phdp) and Vibrio nigripulchritudo, respectively, that acts by targeting NF-kB (with 5 publications, one as 1st /corresponding author). He has established collaborative work with another groups (3 publications), and participated in a variety of other projects involved in the characterization of novel virulence factors secreted by Phdp which is responsible for fish photobacteriosis (one publication published as 1st /corresponding author). He has co-supervised undergraduate, master and PhD students and has taken roles of lab management, budgeting and logistics. He also gave 15 oral presentation and 43 posters in national/international conferences, meetings, seminars and symposiums and published 3 conference papers. Since January 2025, he initiated a new exciting project in the Molecular Microbiology Group at i3S, which aims to reveal the role of surface glycosylations in the virulence and resistance to antibiotics of Enterococcus faecalis, a major Gram+ multidrug-resistant pathogen.
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Identification

Personal identification

Full name
Johnny Lisboa

Citation names

  • Lisboa, Johnny

Author identifiers

Ciência ID
4018-10EA-5F3E
ORCID iD
0000-0001-7329-8993
Researcher Id
V-9696-2019
Scopus Author Id
55250855500

Email addresses

  • johnny.lisboa@i3s.up.pt (Professional)

Telephones

Telephone
  • 220408800 Ext.: 6100/6097 (Professional)

Addresses

  • i3S - Instituto de Investigação e Inovação em Saúde. Rua Alfredo Allen, 208, 4200-135, Porto, Porto, Portugal (Professional)

Websites

Knowledge fields

  • Natural sciences - Biological Sciences - Biophysics
  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Biochemistry

Languages

Language Speaking Reading Writing Listening Peer-review
French (Mother tongue)
Portuguese (Mother tongue)
Spanish; Castilian Upper intermediate (B2) Upper intermediate (B2) Intermediate (B1) Advanced (C1) Upper intermediate (B2)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2010/10/01 - 2013/12/18
Concluded
 Biochemistry and Biophysics (Doctorat)
Université Paris-Sud, France

Institut de Biochimie et Biophysique Moléculaire et Cellulaire, France
"Structural and Functional studies of DprA and partners during natural genetic transformation" (THESIS/DISSERTATION)
 Honnors
2008/09/01 - 2010/06/30
Concluded
 Science and Technology - Master of Molecular and Cellular Biology (Maîtrise)
Major in Biochemistry and Molecular Biology
Universite de Paris VI (Pierre et Marie Curie), France
"Topology and function study of MFS transporters (Major Facilitator Superfamily) of the family DHA3 involved in the macrolide resistance (antibiotics)" (THESIS/DISSERTATION)
 5th out of 15
2005/09/01 - 2008/06/30
Concluded
 Science and Technology - Life and earth sciences (Licence)
Université Paris-Est Créteil Val de Marne, France
"Determination of the precursor cells in the liver fibrogenesis" (THESIS/DISSERTATION)
 34th out of 132
2002/09/01 - 2005/06/30
Concluded
 Bacharelato em Ciência (Bachelor)
Lycée d'Arsonval, France
 N.A.
Affiliation

Science

Category
Host institution 		Employer
2018/07/15 - Current Contracted Researcher (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2014/05/01 - 2018/07/14 Researcher (Research) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015/09/05 - 2015/09/30 Research Trainee (Research) Jacobs University Bremen gGmbH, Germany
2010/10/01 - 2013/12/18 Researcher (Research) Institut de Biochimie et Biophysique Moléculaire et Cellulaire, France
2010/01/01 - 2010/07/31 Research Trainee (Research) Institut Pasteur, France
2009/02/01 - 2009/04/30 Research Trainee (Research) École Normale Supérieure Paris-Saclay, France
2008/04/01 - 2008/07/31 Research Trainee (Research) Institut Mondor de recherche biomédicale, France
Projects

Grant

Designation Funders
2024/01/01 - Current Blocking bacterial glycosyltransferases to circumvent antibiotic resistance
HR23-00682
Researcher
Caixa d'Estalvis i Pensions de Barcelona
Ongoing
2023/01/01 - Current IGNITION: Improving GreeN Innovation for the blue revoluTION: new tools and opportunities for a more sustainable animal farming
101084651
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Horizon 2020
Ongoing
2021/03/28 - Current PhotoVac: T3SS and T5SS in Photobacterium damselae subsp. piscicida: from basic studies to vaccine development
165189PRJ
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2021/03/28 - Current T3SS and T5SS in Photobacterium damselae subsp. piscicida: from basic studies to vaccine development
PTDC/CVT-CVT/7177/2020
10.54499/PTDC/CVT-CVT/7177/2020
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2021/01/01 - 2025/12/31 Institute for Research and Innovation in Health
LA/P/0070/2020
Researcher
Instituto Nacional de Engenharia Biomédica, Portugal

Universidade do Porto, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/07/15 - 2021/07/14 Identification and characterization of virulence factors secreted by Photobacterium damselae piscicida with potential to be used as antigens for vaccination against fish photobacteriosis
151209PRJ
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2016/05/01 - 2018/07/14 AIP56 as an antigen-delivery platform
NORTE-01-0145-FEDER-000012
Post-doc Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2016/01/01 - 2016/04/30 Host cell factors and molecular mechanisms implicated in intoxication by AIP56, a bacterial toxin targeting NF-kB
148251PRJ
Post-doc Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2015/10/01 - 2015/12/31 Structural determinants required for receptor binding and membrane translocation of AIP56
Incentivo/SAU/LA0002/2014
Post-doc Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2015/05/15 - 2015/09/30 Studies towards identification of structural determinants required for receptor binding and membrane translocation of AIP56, a Photobacterium damselae subsp. piscicida apoptogenic AB toxin targeting NF-kB
129800PRJ
Post-doc Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2014/05/01 - 2015/05/14 Host-Pathogen Interactions
NORTE-07-0124-FEDER-000002
Post-doc Fellow
Fundação para a Ciência e a Tecnologia
Concluded
2010/10/01 - 2013/12/19 Dynamique des génomes de bactériophages
ANR-10-BLAN-1328
PhD Student Fellow
Agence nationale de la recherche
Concluded

Contract

Designation Funders
2025/01 - Current Blocking bacterial glycosyltransferases to circumvent antibiotic resistance
HR23-00682
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Caixa d'Estalvis i Pensions de Barcelona
Ongoing
2023/01/01 - 2024/12/31 IGNITION: Improving GreeN Innovation for the blue revoluTION: new tools and opportunities for a more sustainable animal farming
101084651
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Horizon 2020
Ongoing
2021/07/15 - 2022/07/14 PhotoVac: T3SS and T5SS in Photobacterium damselae subsp. piscicida: from basic studies to vaccine development
165189PRJ
Researcher
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018/07/15 - 2022/07/14 Structural and functional characterization of AB toxins targeting antigen-presenting cells and their application as cancer vaccines
151103PRJ
Researcher
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018/07/15 - 2022/07/14 Identification and characterization of virulence factors secreted by Photobacterium damselae piscicida with potential to be used as antigens for vaccination against fish photobacteriosis
151209PRJ
Researcher
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2019/01/01 - 2019/12/31 Instituto de Investigação e Inovação em Saúde
UID/BIM/04293/2019
Researcher
Instituto Nacional de Engenharia Biomédica, Portugal

Universidade do Porto, Portugal

Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016/06/01 - 2019/12/01 Host cell factors and molecular mechanisms implicated in intoxication by AIP56, a bacterial toxin targeting NF-kB
148251PRJ
Researcher
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2016/05/01 - 2019/04/30 Structured Program on Bioengineered Therapies for Infectious Diseases and Tissue Regeneration
NORTE-01-0145-FEDER-000012
Post-doc Fellow
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2013/12/01 - 2015/06/30 Host-Pathogen Interactions
NORTE-07-0124-FEDER-000002
Researcher
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2010/10/01 - 2013/12/18 French Infrastructure for Integrated Structural Biology
ANR-10-INSB-05-01
PhD Student Fellow
Agence nationale de la recherche
Concluded
2008/04/01 - 2008/08/31 IMMUNITE INNEE ET FIBROGENESE HEPATIQUES : LE PARADIGME PORTAL
ANR-06-PHYS-0022
Other
INSERM, France
 Agence Nationale pour la Recherche
Concluded
Outputs

Publications

Conference paper
  1. Lisboa, Johnny; D. Pinto, Rute; Rodrigues, Inês S.; Pereira, Liliana M.G.; Pereira, Cassilda; Oliveira, Pedro; Pereira, J.B. Pedro; et al. Corresponding author: Lisboa, Johnny. "3D structure of the NF-kB targeting toxin AIP56 and characterization of determinants required for low pH-induced conformational changes, membrane interaction and translocation". Paper presented in IUBMB-FEBS-PABMB 2022 Congress: 'The Biochemistry Global Summit', Lisbon, 2022.
    Published
  2. Celma, Louisa; Philippe, Cuniasse; Durand, Dominique; Lisboa, Johnny; Sanchez, Dyana; Marsin, Stéphanie; Quevillon-Cheruel, Sophie; Radicella, Pablo. "HpDprA domains involved in interaction with DNA". Paper presented in 31st European Crystallographic Meeting, Oviedo, 2018.
    Published • 10.1107/s2053273318092124
  3. Lisboa, Johnny; Andreani, Jessica; Marsin, Stéphanie; Ochsenbein, Françoise; Guerois, Raphael; Radicella, Pablo; van Tilbeurgh, Herman; Quevillon-Cheruel, Sophie. "Natural genetic transformation in H. pylori: DprA interactome role". Paper presented in 22nd IUBMB, 37th FEBS and 35th SEBBM Congresses, Seville, 2012.
    Published • 10.1111/j.1742-4658.2010.08705.x
Conference poster
  1. Meireles, Diana; Lisboa, Johnny; Pombinho R.; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. "Biochemical insights into CadC: A metal responsive regulator of virulence in Listeria monocytogenes". Paper presented in EMBL Symposium, Infection: pathogens, hosts, and microbiomes, 2025.
  2. Meireles, Diana; Lisboa, Johnny; Pombinho, Rita; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. "Biochemical insights into CadC: A metal-responsive regulator of virulence in Listeria monocytogenes". Paper presented in 11th Molecular and Cell Biology Symposium, 2025.
  3. Lisboa, Johnny; Freitas, Inês Lua; do Vale, Ana; dos Santos, Nuno M.S.. "AIP56 and AIP56-like toxins: a family of bacterial toxins that target NF-kB". Paper presented in 11th Molecular and Cell Biology Symposium, 2025.
  4. Lisboa, Johnny; Freitas, Inês Lua; do Vale, Ana; dos Santos, Nuno M.S.. "AIP56 and AIP56-like toxins: a family of bacterial toxins that target NF-kB". Paper presented in Era CHAIRS Annual Meeting ImmunoHUB & NCBIO, 2024.
  5. Meireles, Diana; Pombinho R.; Lisboa, Johnny; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. "Biochemical characterization of CadC: a key transcriptional regulator of Listeria monocytogenes virulence factors". Paper presented in 21st International Symposium on Problems of Listeria and Listeriosis (ISOPOL), 2024.
  6. Meireles, Diana; Pombinho R.; Lisboa, Johnny; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. "Unveiling the biochemical mechanisms of the Listeria monocytogenes transcriptional repressor CadC". Paper presented in One Health PhD Forum, 2024.
  7. Meireles, Diana; Pombinho R.; Lisboa, Johnny; Monteiro, Ricardo; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. "Unravelling signals underlying Listeria monocytogenes cadC regulation". Paper presented in European Congress of Clinical Microbiology and Infectious Diseases, 2024.
  8. Meireles, Diana; Pombinho R.; Lisboa, Johnny; Monteiro, Ricardo; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. "Uncovering the signals behind the expression of the Listeria monocytogenes regulator CadC". Paper presented in 10th Molecular and Cell Biology Symposium, 2024.
  9. Molina-Sorribes, C; Lisboa, Johnny; Sanjuan, E; Fouz, B; do Vale, Ana; Amaro, C. "A new vaccine against the zoonotic pathogen Vibrio vulnificus: First Step". Paper presented in Aquaculture Europe 2023, 2023.
  10. Pinto, Alexandre Jorge; Lisboa, Johnny; Barca, Alba V.; Terceti, Mateus S.; Azevedo, Jorge E; Osorio, Carlos R.; dos Santos, Nuno M.S.; do Vale, Ana. "The two-component system RstAB regulates toxin expression in Photobacterium damselae subsp. piscicida". Paper presented in ETOX23 - European Workshop on Bacterial Protein Toxins, 2023.
  11. Lisboa, Johnny; Pereira, Cassilda; Bruno Pinheiro; D. Pinto, Rute; Rodrigues, Inês S.; Pereira, Liliana M.G.; Oliveira, Pedro N.; et al. "Three-dimensional structure and mechanisms of membrane interaction and translocation of the NF-kB-targeting toxin AIP56". Paper presented in ETOX23 - European Workshop on Bacterial Protein Toxins, 2023.
  12. Bruno Pinheiro; Moura, Ana; Lisboa, Johnny; Azevedo, Jorge E; do Vale, Ana; dos Santos, Nuno M.S.. "Development of tools towards the use of AIP56 as a vehicle for the intracellular delivery of antigens in antigen-presenting cells". Paper presented in ETOX23 - European Workshop on Bacterial Protein Toxins, 2023.
  13. José Viveiros; Lisboa, Johnny; Teixeira, Alexandra; Loureiro, Inês; dos Santos, Nuno M.S.; do Vale, Ana. "Recombinant expression and purification of trimeric autotransporter adhesins from Photobacterium damselae subsp. piscicida". Paper presented in IJUP - Encontro de Investigação Jovem, 2023.
  14. Bruno Pinheiro; Moura, Ana; Lisboa, Johnny; Azevedo, Jorge E; do Vale, Ana; dos Santos, Nuno M.S.. "Development of tools towards the use of AIP56 as a vehicle for the intracellular delivery of antigens in antigen-presenting cells". Paper presented in XLVIII Annual Meeting of the Portuguese Society for Immunology, 2023.
  15. Lisboa, Johnny; Freitas, Inês Lua; do Vale, Ana; dos Santos, Nuno M.S.. "AIP56 and AIP56-like toxins: a family of bacterial toxins that target NF-kB". Paper presented in XLVIII Annual Meeting of the Portuguese Society for Immunology, 2023.
  16. Lisboa, Johnny; Freitas, Inês Lua; Pereira, Cassilda; Bruno Pinheiro; D. Pinto, Rute; Inês S. Rodrigues; Pereira, Liliana M.G.; et al. "AIP56 and AIP56-like toxins: a family of bacterial toxins that target NF-kB". Paper presented in 1st ImmunoHUB Annual Meeting, 2023.
  17. Bruno Pinheiro; Moura, Ana; Lisboa, Johnny; Azevedo, Jorge E; do Vale, Ana; dos Santos, Nuno M.S.. "Development of tools towards the use of AIP56 as a vehicle for the intracellular delivery of antigens in antigen-presenting cells". Paper presented in 1st ImmunoHUB Annual Meeting, 2023.
  18. Bruno Miguel Silva Pinheiro; Moura, Ana; Lisboa, Johnny; Azevedo, Jorge E; do Vale, Ana; dos Santos, Nuno M.S.. "Antigen Delivery by AB toxins". Paper presented in 9th McBiology Symposium, 2023.
  19. Lisboa, Johnny; D. Pinto, Rute; Rodrigues, Inês S.; Pereira, Liliana M.G.; Pereira, Cassilda; Oliveira, Pedro; Pereira, J.B. Pedro; et al. "3D structure of the NF-kB targeting toxin AIP56 and characterization of determinants required for low pH-induced conformational changes, membrane interaction and translocation". Paper presented in IUBMB-FEBS-PABMB 2022 Congress: 'The Biochemistry Global Summit', 2022.
  20. Pinto, AJ; Lisboa, Johnny; do Vale, Ana; dos Santos, Nuno M.S.. "Role of RstAB in the regulation of toxin expression by Photobacterium damselae subsp. piscicida". Paper presented in 8th Molecular and Cell Biology Symposium, 2022.
  21. Lisboa, Johnny; Freitas, Inês Lua; do Vale, Ana; dos Santos, Nuno M.S.. "NiTox: a new Vibrio Nigripulchritudo AB-toxin". Paper presented in ETOX2021 - European Workshop on Bacterial Protein Toxins, 2021.
  22. Ana Moura; Lisboa, Johnny; Azevedo, JE; do Vale, Ana; Nuno Miguel Simões dos Santos. "Protein-protein fusion techniques: a new approach to generate AIP56 chimeras for antigen delivery". Paper presented in VI Jornadas da Bioquímica, 2021.
  23. Freitas, Inês Lua; Lisboa, Johnny; Ana do vale; Nuno MS dos Santos. "AIP56 and AIP56-related toxins: common receptor, different functions". Paper presented in 7th Molecular and Cell Biology Symposium, 2021.
  24. Lisboa, Johnny; Andreia Fernandes; Freitas, Inês Lua; Ana do Vale; Nuno MS dos Santos. "Structural insights on AIP56 and AIP56-related toxins". Paper presented in 8th i3S Annual Meeting, 2019.
  25. Inês Marina Soares Loureiro; Alexandra Teixeira; Lisboa, Johnny; A.V. Barca; Osorio, Carlos R.; Fatima Gärtner; Nuno MS dos Santos; Ana do Vale. "Photobacterium deadly duet: uncovering the mechanism of action of a putative binary pore-forming toxin". Paper presented in 8th i3S Annual Meeting, 2019.
  26. Bruno Miguel Silva Pinheiro; Inês Lua Araújo Dias Nunes de Freitas (AB10-8825-5AAD); Lisboa, Johnny; Rodrigues, Inês S.; Do Vale, A.; Dos Santos, N.M.S.. "Development of an antigen delivery platform based on AIP56". Paper presented in YES Meeting, 2019.
  27. Bruno Miguel Silva Pinheiro; Inês Lua Araújo Dias Nunes de Freitas (AB10-8825-5AAD); Lisboa, Johnny; Rodrigues, Inês S.; Do Vale, A.; Dos Santos, N.M.S.. "Development of an antigen delivery platform based on AIP56". Paper presented in 3RD Symposium on Immunomodulation in Cancer & Regeneration, 2019.
  28. Lisboa, Johnny; Fernandes, Andreia; Inês Lua Araújo Dias Nunes de Freitas (AB10-8825-5AAD); Do Vale, A.; Dos Santos, N.M.S.. "Structural insights on AIP56 and AIP56-related toxins". Paper presented in ETOX19 - European Workshop on Bacterial Protein Toxins, 2019.
  29. Lisboa, Johnny; Pereira, Cassilda; Ayala, Juan; Terceti, Mateus; Rodrigues, Inês S.; Pereira, J.B. Pedro; Osorio, C.R.; et al. "Characterization of a novel NlpC/P60 endopeptidase of Photobacterium damselae subsp. piscicida secreted by the Type II secretion system". Paper presented in 7th i3S Annual Meeting, 2018.
  30. Lisboa, Johnny; Pereira, Cassilda; Ayala, Juan; Terceti, Mateus; Rodrigues, Inês S.; Pereira, J.B. Pedro; Osorio, C.R.; et al. "Characterization of a novel NlpC/P60 endopeptidase of Photobacterium damselae subsp. piscicida secreted by the Type II secretion system". Paper presented in Bacterial Protein Export 2018, 2018.
  31. Campos Pinheiro, Jorge Nuno Martins; Lisboa, Johnny; Filipe Freitas Coelho Henriques de Carvalho; Carreaux, Alexis; Nuno MS dos Santos; Sandra Sousa; Didier Cabanes. "LarS controls the expression of the Agr system of Listeria monocytogenes and mediates virulence". Paper presented in 7th Congress of European Microbiologists - FEMS 2017, 2017.
  32. Lisboa, Johnny; Rodrigues, Inês S.; Benz, Roland; Do Vale, A.; Dos Santos, N.M.S.. "Structural determinants required for pH-induced unfolding and pore formation in AIP56". Paper presented in ETOX18 – European Workshop on Bacterial Protein Toxins, 2017.
  33. Rodrigues, Inês S.; Pereira, Cassilda; Pereira, Liliana M.G.; Lisboa, Johnny; Dos Santos, N.M.S.; Do Vale, A.. "Host factors involved in AIP56 intoxication: the role of protein chaperones and redox systems". Paper presented in ETOX18 – European Workshop on Bacterial Protein Toxins, 2017.
  34. Lisboa, Johnny; Rodrigues, Inês S.; Pereira, Liliana M.G.; Pinto, Rute D.; Peres, J.; Do Vale, A.; Dos Santos, N.M.S.. "AIP56: a short-trip AB toxin with a peculiar translocation domain". Paper presented in I3S Retreat- 5th Annual Meeting, 2016.
  35. Rodrigues, Inês S.; Pereira, Liliana M.G.; Pinto, Rute D.; Lisboa, Johnny; Dos Santos, N.M.S.; Do Vale, A.. "Involvement of Hsp90 and PPIases in AIP56 intoxication". Paper presented in I3S Retreat- 5th Annual Meeting, 2016.
  36. Pereira, Liliana M.G.; Pinto, Rute D.; Peres, J.; Rodrigues, Inês S.; Lisboa, Johnny; Dos Santos, N.M.S.; Do Vale, A.. "Involvement of Hsp90 and PPIases in AIP56 intoxication". Paper presented in ETOX17 - 17th European Workshop on Bacterial Protein Toxins, 2015.
  37. Peres, J.; Rodrigues, Inês S.; Pereira, Liliana M.G.; Pinto, Rute D.; Lisboa, Johnny; Do Vale, A.; Dos Santos, N.M.S.. "AIP56: a short-trip AB toxin with a peculiar translocation domain". Paper presented in ETOX17 - 17th European Workshop on Bacterial Protein Toxins, 2015.
  38. Lisboa, Johnny; Ochsenbein, Françoise; Durand, Dominique; van Tilbeurgh, Herman; Guerois, Raphael; Quevillon-Cheruel, Sophie. "The natural transformation in H. pylori: structural study of DprA interactome". Paper presented in AFC Meeting, 2013.
  39. Lisboa, Johnny; Ochsenbein, Françoise; Durand, Dominique; van Tilbeurgh, Herman; Guerois, Raphael; Quevillon-Cheruel, Sophie. "The natural transformation in H. pylori: structural study of DprA interactome". Paper presented in The 10th Meeting of 3R, 2013.
  40. Lisboa, Johnny; Ochsenbein, Françoise; Durand, Dominique; van Tilbeurgh, Herman; Guerois, Raphael; Quevillon-Cheruel, Sophie. "The natural transformation in H. pylori: structural study of DprA interactome". Paper presented in The 4th Microbial Genome Maintenance Meeting: Transformation and DNA Repair, 2013.
  41. Lisboa, Johnny; Andreani, Jessica; Prado-Acosta, Mariano; Marsin, Stéphanie; Ochsenbein, Françoise; Guerois, Raphael; Durand, Dominique; et al. "DprA role in the natural genetic transformation in H. pylori". Paper presented in The 4th EMBO Meeting, 2012.
  42. Lisboa, Johnny; Andreani, Jessica; Prado-Acosta, Mariano; Marsin, Stéphanie; Ochsenbein, Françoise; Guerois, Raphael; Durand, Dominique; et al. "DprA role in the natural genetic transformation in H. pylori". Paper presented in From Single Molecules to Systems Biology - 22nd IUBMB & 37th FEBS Congress, 2012.
  43. Lisboa, Johnny; Andreani, Jessica; Marsin, Stéphanie; Ochsenbein, Françoise; Guerois, Raphael; Durand, Dominique; van Tilbeurgh, Herman; Radicella, Pablo; Quevillon-Cheruel, Sophie. "Natural genetic transformation in H. pylori: research for functional and structural peculiarities of DprA". Paper presented in 12th Meeting of the Doctoral School, Therapeutic Innovation, 2012.
  44. Lisboa, Johnny; Andreani, Jessica; Marsin, Stéphanie; Ochsenbein, Françoise; Guerois, Raphael; Durand, Dominique; van Tilbeurgh, Herman; Radicella, Pablo; Quevillon-Cheruel, Sophie. "Natural genetic transformation in H. pylori: research for functional and structural peculiarities of DprA". Paper presented in GTBio 2012, 2012.
Journal article
  1. Mariana Morais; Francisca Dias; Patrícia Figueiredo; Inês Tavares; Carla Escudeiro; Manuel Teixeira; Alexandra Teixeira; et al. "Silver Nanoparticles (AgNPs) Uptake by Caveolae-Dependent Endocytosis is Responsible for Their Selective Effect Towards Castration Resistant Prostate Cancer". International Journal of Nanomedicine (2024): https://doi.org/10.2147/IJN.S447645.
    Published • 10.2147/IJN.S447645
  2. Lisboa, Johnny; Pereira, Cassilda; Pinto, Rute D.; Rodrigues, Inês S.; Pereira, Liliana M. G.; Pinheiro, Bruno; Oliveira, Pedro; et al. Corresponding author: Lisboa, Johnny. "Unconventional structure and mechanisms for membrane interaction and translocation of the NF-kB-targeting toxin AIP56". Nature Communications 14 1 (2023): http://dx.doi.org/10.1038/s41467-023-43054-z.
    Published • 10.1038/s41467-023-43054-z
  3. Teixeira, Alexandra; Loureiro, Inês; Lisboa, Johnny; Oliveira, Pedro N.; Azevedo, Jorge E.; dos Santos, Nuno M. S.; do Vale, Ana. "Characterization and Vaccine Potential of Outer Membrane Vesicles from Photobacterium damselae subsp. piscicida". International Journal of Molecular Sciences 24 6 (2023): 5138. http://dx.doi.org/10.3390/ijms24065138.
    Published • 10.3390/ijms24065138
  4. Freitas, Inês Lua; Teixeira, Alexandra; Loureiro, Inês; Lisboa, Johnny; Saraiva, Aurélia; dos Santos, Nuno Miguel Simões; do Vale, Ana. "Susceptibility of Sea Bream (Sparus aurata) to AIP56, an AB-Type Toxin Secreted by Photobacterium damselae subsp. piscicida". Toxins 14 2 (2022): 119. http://dx.doi.org/10.3390/toxins14020119.
    Published • 10.3390/toxins14020119
  5. Lisboa, Johnny; Pereira, Cassilda; Rifflet, Aline; Ayala, Juan; Terceti, Mateus S.; Barca, Alba V.; Rodrigues, Inês; et al. Corresponding author: Lisboa, Johnny. "A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida Cleaves the Peptidoglycan of Potentially Competing Bacteria". mSphere 6 1 (2021): http://dx.doi.org/10.1128/msphere.00736-20.
    Published • 10.1128/msphere.00736-20
  6. Ithurbide, Solenne; Coste, Geneviève; Lisboa, Johnny; Eugénie, Nicolas; Bentchikou, Esma; Bouthier de la Tour, Claire; Liger, Dominique; et al. "Natural Transformation in Deinococcus radiodurans: A Genetic Analysis Reveals the Major Roles of DprA, DdrB, RecA, RecF, and RecO Proteins". Frontiers in Microbiology 11 (2020): http://dx.doi.org/10.3389/fmicb.2020.01253.
    Published • 10.3389/fmicb.2020.01253
  7. Rodrigues, Inês S.; pereira, Liliana M. G.; Lisboa, Johnny; Pereira, Cassilda; Oliveira, Pedro; Santos, Nuno M. S. dos; Vale, Ana do. "Author Correction: Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida". Scientific Reports 10 1 (2020): http://dx.doi.org/10.1038/s41598-020-58152-x.
    Published • 10.1038/s41598-020-58152-x
  8. Inês S. Rodrigues; Liliana M. G. Pereira; Johnny Lisboa; Cassilda Pereira; Pedro Oliveira; Nuno M. S. dos Santos; Ana do Vale. "Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida". Scientific Reports 9 1 (2019): https://doi.org/10.1038/s41598-019-45240-w.
    Published • 10.1038/s41598-019-45240-w
  9. Pereira, Cassilda; Rodrigues, Inês S.; Pereira, Liliana M.G.; Lisboa, Johnny; Pinto, Rute D.; Araújo, Leonor; Oliveira, Pedro; et al. "Role of AIP56 disulphide bond and its reduction by cytosolic redox systems for efficient intoxication". Cellular Microbiology 22 1 (2019): http://dx.doi.org/10.1111/cmi.13109.
    Published • 10.1111/cmi.13109
  10. Terceti, Mateus S.; Vences, Ana; Matanza, Xosé M.; Barca, Alba V.; Noia, Manuel; Lisboa, Johnny; dos Santos, Nuno M. S.; do Vale, Ana; Osorio, Carlos R.. "The RstAB System Impacts Virulence, Motility, Cell Morphology, Penicillin Tolerance and Production of Type II Secretion System-Dependent Factors in the Fish and Human Pathogen Photobacterium damselae subsp. damselae". Frontiers in Microbiology 10 (2019): http://dx.doi.org/10.3389/fmicb.2019.00897.
    Published • 10.3389/fmicb.2019.00897
  11. Lisboa, Johnny; Celma, Louisa; Sanchez, Dyana; Marquis, Mathilde; Andreani, Jessica; Guérois, Raphael; Ochsenbein, Françoise; et al. "The C-terminal domain of HpDprA is a DNA-binding winged helix domain that does not bind double-stranded DNA". The FEBS Journal 286 10 (2019): 1941-1958. http://dx.doi.org/10.1111/febs.14788.
    10.1111/febs.14788
  12. Pinheiro, Jorge; Lisboa, Johnny; Pombinho, Rita; Carvalho, Filipe; Carreaux, Alexis; Brito, Cláudia; Pöntinen, Anna; et al. "MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence". Nucleic Acids Research (2018): http://dx.doi.org/10.1093/nar/gky624.
    10.1093/nar/gky624
  13. Devigne, Alice; Guérin, Philippe; Lisboa, Johnny; Quevillon-Cheruel, Sophie; Armengaud, Jean; Sommer, Suzanne; Bouthier de la Tour, Claire; Servant, Pascale. "PprA Protein Is Involved in Chromosome Segregation via Its Physical and Functional Interaction with DNA Gyrase in Irradiated Deinococcus radiodurans Bacteria". mSphere 1 1 (2016): http://dx.doi.org/10.1128/msphere.00036-15.
    10.1128/msphere.00036-15
  14. Serre, Marie-Claude; El Arnaout, Toufic; Brooks, Mark A.; Durand, Dominique; Lisboa, Johnny; Lazar, Noureddine; Raynal, Bertrand; van Tilbeurgh, Herman; Quevillon-Cheruel, Sophie. "Correction: The Carboxy-Terminal aN Helix of the Archaeal XerA Tyrosine Recombinase Is a Molecular Switch to Control Site-Specific Recombination". PLoS ONE 9 1 (2014): http://dx.doi.org/10.1371/annotation/ad556340-dea0-40c9-b90c-43198d427095.
    Published • 10.1371/annotation/ad556340-dea0-40c9-b90c-43198d427095
  15. Lisboa, J.; Andreani, J.; Sanchez, D.; Boudes, M.; Collinet, B.; Liger, D.; Tilbeurgh, H. v.; Guerois, R.; Quevillon-Cheruel, S.. "Molecular determinants of the DprA-RecA interaction for nucleation on ssDNA". Nucleic Acids Research 42 11 (2014): 7395-7408. https://doi.org/10.1093/nar/gku349.
    Published • 10.1093/nar/gku349
  16. Serre, Marie-Claude; El Arnaout, Toufic; Brooks, Mark A.; Durand, Dominique; Lisboa, Johnny; Lazar, Noureddine; Raynal, Bertrand; et al. "The Carboxy-Terminal aN Helix of the Archaeal XerA Tyrosine Recombinase Is a Molecular Switch to Control Site-Specific Recombination". PLoS ONE 8 5 (2013): e63010-e63010. https://doi.org/10.1371/journal.pone.0063010.
    Published • 10.1371/journal.pone.0063010
  17. Quevillon-Cheruel, S.; Campo, N.; Mirouze, N.; Mortier-Barriere, I.; Brooks, M. A.; Boudes, M.; Durand, D.; et al. "Structure-function analysis of pneumococcal DprA protein reveals that dimerization is crucial for loading RecA recombinase onto DNA during transformation". Proceedings of the National Academy of Sciences 109 37 (2012): E2466-E2475. https://doi.org/10.1073/pnas.1205638109.
    Published • 10.1073/pnas.1205638109
  18. Durand, Dominique; Li de la Sierra-Gallay, Ines; Brooks, Mark A.; Thompson, Andrew W.; Lazar, Noureddine; Lisboa, Johnny; van Tilbeurgh, Herman; Quevillon-Cheruel, Sophie. "Expression, purification and preliminary structural analysis of Escherichia coli MatP in complex with the matS DNA site". Acta Crystallographica Section F Structural Biology and Crystallization Communications 68 6 (2012): 638-643. https://doi.org/10.1107/S1744309112011062.
    Published • 10.1107/S1744309112011062
Online resource
  1. Lisboa, Johnny; Pereira, P.J.B.; Dos Santos, N.M.S.. Three-dimensional structure of AIP56, a short-trip single chain AB toxin from Photobacterium damselae subsp. piscicida [7ZPF]. 2023. https://www.rcsb.org/structure/7ZPF.
    https://doi.org/10.2210/pdb7ZPF/pdb
  2. Lisboa, Johnny; Durand, Dominique. SASDNW6 – AIP56, an AB-toxin from Photobacterium damselae subsp. piscicida. 2023. https://www.sasbdb.org/data/SASDNW6/.
  3. Lisboa, Johnny; Pereira, P.J.B.; Dos Santos, N.M.S.. Insights into a novel NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida [6SQX]. 2020. https://www.rcsb.org/structure/6SQX.
    https://doi.org/10.2210/pdb6SQX/pdb
  4. Lisboa, Johnny; Celma, Louisa; Sanchez, D.; Marquis, M. ; Andreani, Jessica; Guerois, Raphael; Ochsenbein, Françoise; et al. The CTD of HpDprA, a DNA binding Winged Helix domain which do not bind dsDNA [6GW7]. 2019. https://www.rcsb.org/structure/6GW7.
    https://doi.org/10.2210/pdb6GW7/pdb
  5. Lisboa, Johnny; Dos Santos, N.M.S.. Cyclophilin A [6FK1]. 2019. https://www.rcsb.org/structure/6FK1.
    https://doi.org/10.2210/pdb6FK1/pdb
  6. Campos Pinheiro, J.; Lisboa, Johnny; Carvalho, F.; Carreaux, A.; Dos Santos, N.M.S.; Morais-Cabral, J.H.; Sousa, Sandra; Cabanes, Didier. Lar controls the expression of the Listeria monocytogenes agr system and mediates virulence [6EP3]. 2018. https://www.rcsb.org/structure/6EP3.
    https://doi.org/10.2210/pdb6EP3/pdb
  7. Lisboa, Johnny; Quevillon-Cheruel, S.. Structure of HpDprA at 1.9 Angstroms resolution [5MLL]. 2017. https://www.rcsb.org/structure/5MLL.
    https://doi.org/10.2210/pdb5MLL/pdb
  8. Brooks, M.A.; El Arnaout, T.; Durand, D.; Lisboa, Johnny; Lazar, N.; Raynal, B.; van Tilbeurgh, H.; Serre, M.; Quevillon-Cheruel, S.. The structure of a dimeric Xer recombinase from archaea [4A8E]. 2012. https://www.rcsb.org/structure/4A8E.
    https://doi.org/10.2210/pdb4A8E/pdb
Preprint
  1. Lisboa, Johnny; Pereira, Cassilda; Pinto, Rute D.; Rodrigues, Inês S.; Pereira, Liliana M. G.; Pinheiro, Bruno; Oliveira, Pedro; et al. "Structural and functional characterization of the NF-kB-targeting toxin AIP56 from Photobacterium damselae subsp. piscicida reveals a novel mechanism for membrane interaction and translocation". 2023. http://dx.doi.org/10.1101/2023.05.04.539443.
    10.1101/2023.05.04.539443
  2. Johnny Lisboa; Cassilda Pereira; Aline Rifflet; Juan Ayala; Mateus S. Terceti; Alba V. Barca; Inês Rodrigues; et al. "A secreted NlpC/P60 endopeptidase from Photobacterium damselae subsp. piscicida cleaves the peptidoglycan of potentially competing bacteria". 2020. https://doi.org/10.1101/2020.06.30.181511.
    10.1101/2020.06.30.181511
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/11/14 Biochemical characterization of CadC: a key transcriptional regulator of Listeria monocytogenes virulence factors (*Diana Meireles as presenting author) 11th i3S Annual Meeting
i3S (Póvoa de Varzim, Portugal)
2024/11 Photobacterium damselae subsp. piscicida OMVs: turning weapons into pearls (*Ana do Vale as presenting author) International Animal Health Symposium
CIIMAR (Portugal)
2023/09/11 The two-component system RstAB positively regulates the expression of secreted virulence factors in Photobacterium damselae subsp. piscicida (*Ana do Vale as presenting author) 21st International Conference on Diseases of Fish and Shellfish
(Aberdeen)
2023/03/17 PBT: a novel binary toxin from the fish pathogen Photobacterium damselae subsp. piscicida that targets fish, mouse and human macrophages (Best Oral Presentation Award, *Inês Loureiro as presenting author) I ImmunoHUB Annual Meeting - Infection & Immunity
i3S - Instituto de Investigação e Inovação em Saúde (Porto, Portugal)
2022/11/30 AIP56, a single chain AB toxin with a unique structural organization and translocation mechanism MicroHub meeting
CIIMAR (Porto, Portugal)
2022/11/04 Photobacterium deadly duet: activity of a novel virulence-associated binary toxin. (*Inês Loureiro as presenting author) Virtual International Aquaculture and Fisheries Conference
2022/05/04 The two-component system RstAB regulates expression of virulence factors in Photobacterium damselae subsp. piscicida. (*Alexandre Pinto as presenting author) IJUP - Encontro de Investigação Jovem
(Porto, Portugal)
2022/05/02 AIP56 and AIP56-like toxins: a family of bacterial toxins that target NF-kB. (*Inês Lua Freitas as presenting author) 5th European NF-kappaB Subunit Workshop
(Porto, Portugal)
2021/09/20 Structural and functional characterization of a PirAB-like binary toxin from Photobacterium damselae subsp. piscicida. (*Ana do Vale as presenting author) Virtual 20th International Conference on Diseases of Fish and Shellfish
European Association of Fish Pathologists (Virtual)
2021/09/20 Structural and functional characterization of NiTox: a new AB-toxin from Vibrio Nigripulchritudo lethal to shrimp Virtual 20th International Conference on Diseases of Fish and Shellfish
European Association of Fish Pathologists (Virtual)
2021/06/28 Photobacterium deadly duet: structure and activity of a novel virulence-associated binary toxin. (*Ana do Vale as presenting author) ETOX2021 - European Workshop on Bacterial Protein Toxins
Max Planck Institute of Molecular Physiology Dortmund, Germany (Online, Germany)
2021/06/14 AIP56, a single chain AB toxin with a unique structural organization 7th International Iberian Biophysics Congress
University of Coimbra (Porto, Portugal)
2021/05/05 Biotechnological application of AIP56, an AB-toxin from Photobacterium damselae subsp. piscicida. (*Ana Moura as presenting author) IJUP - Encontro de Investigação Jovem
University of Porto (Porto, Portugal)
2019/06/22 Structural insights on AIP56 and AIP56-related toxins. (*Nuno M.S. dos Santos as presenting author) ETOX19 - European Workshop on Bacterial Protein Toxins
(Davos, Switzerland)
2015/09/29 AIP56, an NF-kB cleaving toxin from Photobacterium damselae piscicida (Phdp) MOLIFE RESEARCH SEMINAR
Research II - Lecture Hall (Bremen, Germany)
2013 The natural transformation in H. pylori: structural study of DprA interactome 13th Meeting of the Doctoral School, Therapeutic Innovation
(França, France)
2013 The natural transformation: DprA interactome Frisbi Meeting (French Infrastructure for Integrated Structural Biology)
(CEA Saclay, France)
2013 Conference in a licence class (equivalent to a Bachelor's degree)
(University Paris VI, France)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2021 - Current Characterization of a novel family of AB toxins with potential to be used as antigen delivery vehicles
Co-supervisor of Inês Lua Freitas
 Molecular and Cell Biology (PhD)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2024 - 2025 Structural and functional characterization of trimeric autotransporter adhesins from Photobacterium damselae subsp. piscicida
Co-supervisor
 Master in Biochemistry (Master)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2022 - 2023 Recombinant expression and purification of trimeric autotransporter adhesins from Photobacterium damselae subsp. piscicida
Co-supervisor of José António Gouveia Viveiros
 Degree in Biochemistry (Degree)
Universidade do Porto Faculdade de Ciências, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021 - 2022 Trimeric autotransporter adhesins (TAAs) from Photobacterium damselae subsp. piscicida: investigation of biological functions and recombinant production to use as antigens
Co-supervisor of Gabriela Alves Afonso
 Genética Molecular Comparativa e Tecnológica (Master)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade de Trás-os-Montes e Alto Douro, Portugal
2021 - 2022 Studies on the regulation of Photobacterium damselae subsp. piscicida toxins by the two-component system RstAB
Co-supervisor of Alexandre Pinto
 Master in Biochemistry (Master)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021 - 2021 The understanding of AIP56-like proteins
Supervisor of Clothilde Colart
 Magistère Européen de Génétique (Master)
Université de Paris, France
2020 - 2021 AIP56 and AIP56-related toxins as antigen delivery platforms
Co-supervisor of Ana Moura
 Master in Biochemistry (Master)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019 - 2020 Studies on the regulation of expression of AIP56 by RstA of Photobacterium damselae subsp. piscicida
Co-supervisor of Alexandre Pinto
 Degree in Biochemistry (Degree)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017 - 2018 AIP56, an AB toxin from the fish pathogenic bacterium Photobacterium damselae subsp. piscicida, as a possible drug and/or antigen delivery system for leishmaniasis
Co-supervisor of Laura Dirkx
 Master in Biomedical Sciences (Master)
Universiteit Antwerpen, Belgium

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017 - 2018 AIP56 as an antigen delivery platform: OVA peptide-AIP56 fusion as a proof of concept
Co-supervisor of Ana Manuela Canha Borges
 Degree in Biology (Degree)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017 - 2018 Comparative analysis of proteins secreted by different strains of Photobacterium damselae subsp. piscicida
Co-supervisor of Patricia Martins Ribeiro
 Degree in Biotechnology (Degree)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
2016 - 2017 Translocation mechanism and biotechnological application of AIP56
Co-supervisor of Diogo Moreira Filipe
 Degree in Biology (Degree)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2016 - 2017 Study towards the Use of AIP56 as an Antigen Delivery Platform
Co-supervisor of Sandra Lindner
 Degree in Biology (Degree)
The Ostbayerische Technische Hochschule Amberg-Weiden (OTH – Technical University of Applied Sciences), Germany

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2012 - 2013 Structural and functional study of ComD-ComE, the two-component signal-transduction system that controls competence of Streptococcus pneumoniae
Co-supervisor of Dyana Sanchez
 Master in Biochemistry (Master)
Institut de Biochimie et Biophysique Moléculaire et Cellulaire, France
2010/03 - 2010/06 Construction of new secretion vectors for Escherichia coli recombinants proteins
Co-supervisor of Cédric Radigue
 Degree in Public Health at the University of Paris XI (Degree)
Institut Pasteur, France

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2015/06/20 - 2015/06/24 ETOX17 - 17th European Workshop on Bacterial Protein Toxins (2015/06/20 - 2015/06/24)
Congress (Member of the Organising Committee)
 Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2014 - Current Active roles in lab management and logistics. Sole responsibility for lab ordering and stocking system. Assistant in lab rota and maintenance management. Assistant in lab budgeting and expenses managing.
Other
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2025 - 2025 Advanced Training Visualizing Molecules: A Dive into CryoEM Technology
Workshop
 Visualizing Molecules: A Dive into CryoEM Technology
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2024 - 2024 Lakeside Conference on Protein Toxins and Effectors 2024
Conference
 Lakeside Conference on Protein Toxins and Effectors 2024
2022 - 2022 IUBMB-FEBS-PABMB 2022 Congress: 'The Biochemistry Global Summit'_Poster Presentation
Congress
 IUBMB-FEBS-PABMB 2022 Congress: 'The Biochemistry Global Summit'
2021 - 2021 7th International Iberian Biophysics Congress_Oral Communication
Congress
 7th International Iberian Biophysics Congress
2021 - 2021 ETOX2021 - European Workshop on Bacterial Protein Toxins_Poster Presentation
Workshop
 ETOX2021 - European Workshop on Bacterial Protein Toxins
2021 - 2021 Virtual 20th International Conference on Diseases of Fish and Shellfish_Oral Communication
Conference
 Virtual 20th International Conference on Diseases of Fish and Shellfish
2019 - 2019 8th i3S Annual Meeting_Poster Presentation
Meeting
 8th i3S Annual Meeting
2018 - 2018 7th i3S Annual Meeting_Poster Presentation
Meeting
 7th i3S Annual Meeting
2017 - 2017 ETOX18 – European Workshop on Bacterial Protein Toxins_Poster Presentation
Workshop
 ETOX18 – European Workshop on Bacterial Protein Toxins
2016 - 2016 I3S Retreat- 5th Annual Meeting_Poster Presentation
Meeting
 I3S Retreat- 5th Annual Meeting
2015 - 2015 ETOX17 - 17th European Workshop on Bacterial Protein Toxins_Poster Presentation
Workshop
 ETOX17 - 17th European Workshop on Bacterial Protein Toxins
2015 - 2015 MOLIFE RESEARCH SEMINAR_Oral Communication
Seminar
 MOLIFE RESEARCH SEMINAR
Jacobs University Bremen gGmbH, Germany
2013 - 2013 The 4th Microbial Genome Maintenance Meeting: Transformation and DNA Repair_Poster Presentation
Conference
 The 4th Microbial Genome Maintenance Meeting: Transformation and DNA Repair
2013 - 2013 The 10th Meeting of 3R_Poster Presentation
Meeting
 The 10th Meeting of 3R
2013 - 2013 AFC Meeting_Poster Presentation
Meeting
 AFC Meeting
2013 - 2013 Frisbi Meeting (French Infrastructure for Integrated Structural Biology)_Oral Communication
Meeting
 Frisbi Meeting (French Infrastructure for Integrated Structural Biology)
2013 - 2013 13th Meeting of the Doctoral School, Therapeutic Innovation_Oral Communication
Meeting
 13th Meeting of the Doctoral School, Therapeutic Innovation
2012 - 2012 Congress of the French biocrystallography group (GTBio)_Poster Presentation
Congress
 Congress of the French biocrystallography group (GTBio)
2012 - 2012 12th Meeting of the Doctoral School, Therapeutic Innovation_Poster Presentation
Meeting
 12th Meeting of the Doctoral School, Therapeutic Innovation
2012 - 2012 From Single Molecules to Systems Biology - 22nd IUBMB & 37th FEBS Congress_Poster Presentation
Congress
 From Single Molecules to Systems Biology - 22nd IUBMB & 37th FEBS Congress
2012 - 2012 The 4th EMBO Meeting_Poster Presentation
Meeting
 The 4th EMBO Meeting

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2016/07/20 Crystallization of New Therapeutic Targets Against Mycobacteria
(Thesis) Main arguer
 Hélio Martins Gil (Other)
Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Universidade do Porto Faculdade de Ciências, Portugal

Ad Hoc journal article review

Journal title (ISSN) Publisher
2025 - Current Virulence (2150-5594) TAYLOR & FRANCIS INC
2025 - Current Microbiology Research (2036-7481) MDPI
2024 - Current International Journal of Molecular Sciences (1422-0067) MDPI
2024 - Current International Journal of Biological Macromolecules (1879-0003) Elsevier
2024 - Current Toxins (2072-6651) MDPI
2022 - Current Journal of Bacteriology (0021-9193) American Society for Microbiology (ASM)
2021 - Current Scientific Reports (2045-2322) Nature Portfolio
2017 - Current Molecular Microbiology (0950-382X) Wiley

Association member

Society Organization name Role
2020/03 - Current Portuguese Biochemical Society (SPB) Member
2020/01/01 - Current American Society for Microbiology (ASM) Postdoctoral Membership
2012 - 2013 French Society for Biochemistry and Molecular Biology (SFBBM) Member
2012 - 2013 French Crystallography Association (AFC) Member

Committee member

Activity description
Role 		Institution / Organization
2011 - 2013 Docs/Postdocs representant in the council of the IBBMC (Orsay, France)
President / Vice-president
 Institut de Biochimie et Biophysique Moléculaire et Cellulaire, France

Conference scientific committee

Conference name Conference host
2021/11/30 - 2021/11/30 4th PhDay: Break borders and build opportunities i3S PhD students

Journal scientific committee

Journal title (ISSN) Publisher
2024 - Current International Journal of Molecular Sciences (1422-0067) MDPI
Distinctions

Award

2024 Top Rated Poster in the 34th Congress of the European Society of Clinical Microbiology and Infectious Diseases (*Diana Meireles as presenting author)
2023 Best Oral Communication in ImmunoHUB Annual Meeting - Infection & Immunity. (*Inês Loureiro as presenting author)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022 Best Poster in 8th Molecular and Cell Biology Symposium. (*Alexandre Pinto as presenting author)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022 "Susceptibility of Sea Bream (Sparus aurata) to AIP56, an AB-Type Toxin Secreted by Photobacterium damselae subsp. piscicida" selected as an Editor's Choice Article
2021 Best Poster in 7th Molecular and Cell Biology Symposium. (*Inês Lua Freitas as presenting author)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Other distinction

2023 Junior Researcher Contract (Bio/Horizon/i3S/0211/2022)
2021 Junior Researcher Contract (PTDC/CVT-CVT/7177/2020)
Fundação para a Ciência e a Tecnologia, Portugal
2018 Junior Researcher Contract (PTDC/BIA-MIC/29910/2017)
Fundação para a Ciência e a Tecnologia, Portugal
2016 Post-Doc Fellowship (NORTE-01-0145-FEDER-000012)
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015 Post-Doc Fellowship (FCOMP-01-0124-FEDER-028364-PTDC/BIA-MIC/3463/2012)
Fundação para a Ciência e a Tecnologia, Portugal
2015 Post-Doc Fellowship (Incentivo/SAU/LA0002/2014)
Fundação para a Ciência e a Tecnologia, Portugal
2014 Post-Doc Fellowship (NORTE-07-0124-FEDER-000002)
2013 JP Ebel
2013 AFC Grant
The French Crystallography Association (AFC), France
2012 Travel Grant
IUBMB/FEBS/SEBBM, Portugal
2010 Doctoral School Grant
Doctoral School, ED425, Portugal
2010 PhD Fellowship