Johnny Lisboa (4018-10EA-5F3E)

Curriculum Vitae

Johnny Lisboa

Johnny Lisboa obtained his Master's Degree in Biochemistry in 2010 by the University Pierre et Marie Curie (Paris, France). In 2013, he obtained his PhD on Biochemistry and Biophysics by University of Paris-Sud, under the supervision of Dr. Sophie Quevillon-Cheruel at the Institute for Integrative Biology of the Cell (I2BC, Paris). His PhD project focus in the structural and functional study of specific proteins involved in the natural transformation systems of various human pathogens, in order to understand their molecular mechanisms and interaction networks. Using X-ray crystallography and NMR as the main technical approach, complemented by a plethora of other biochemical, biophysical, and computational techniques, he has been studying the function of macromolecules and macromolecular complexes from their high-resolution structures. He also used powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. He is currently involved in the functional and structural characterization of AIP56, an apoptogenic bacterial toxin from Photobacterium damselae subsp. piscicida (Phdp) that acts by targeting NF-kB (with 5 publications, one as 1st /corresponding author). He has established collaborative work with another groups (with 3 publications), and participated in a variety of other projects involved in the characterization of novel virulence factors secreted by Phdp which is responsible for fish photobacteriosis (one publication published as 1st /corresponding author). He has co-supervised undergraduate, master and PhD students and has taken roles of lab management, budgeting and logistics. He also gave 7 oral presentation and 39 posters in national/international conferences, meetings, seminars and symposiums and published 3 conference papers. He is currently interested in studying/characterizing new AIP56-related toxins to understand the mechanism of these new toxins and used them as alternative vehicles for antigen delivery.

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Identification

Personal identification

Full name: Johnny Lisboa

Citation names

Lisboa, Johnny

Author identifiers

Ciência ID: 4018-10EA-5F3E
ORCID iD: 0000-0001-7329-8993
Researcher Id: V-9696-2019
Scopus Author Id: 55250855500

Email addresses

johnny.lisboa@ibmc.up.pt (Professional)

Telephones

Telephone

220408800 Ext.: 6100/6097 (Professional)

Addresses

i3S - Instituto de Investigação e Inovação em Saúde. Rua Alfredo Allen, 208, 4200-135, Porto, Porto, Portugal (Professional)

Websites

https://orcid.org/0000-0001-7329-8993 (Professional)
https://www.i3s.up.pt/content/people?id=842&idg=19 (Professional)
https://www.researchgate.net/profile/Johnny_Lisboa (Professional)

Knowledge fields

Natural sciences - Biological Sciences - Biophysics
Natural sciences - Biological Sciences - Molecular Biology
Natural sciences - Biological Sciences - Biochemistry

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
French (Mother tongue)
Portuguese (Mother tongue)
Spanish; Castilian	Upper intermediate (B2)	Upper intermediate (B2)	Intermediate (B1)	Advanced (C1)	Upper intermediate (B2)
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)

Education

	Degree	Classification
2010/10/01 - 2013/12/18 Concluded	Biochemistry and Biophysics (Doctorat) Université Paris-Sud, France Institut de Biochimie et Biophysique Moléculaire et Cellulaire, France "Structural and Functional studies of DprA and partners during natural genetic transformation" (THESIS/DISSERTATION)	Honnors
2008/09/01 - 2010/06/30 Concluded	Science and Technology - Master of Molecular and Cellular Biology (Maîtrise) Major in Biochemistry and Molecular Biology Universite de Paris VI (Pierre et Marie Curie), France "Topology and function study of MFS transporters (Major Facilitator Superfamily) of the family DHA3 involved in the macrolide resistance (antibiotics)" (THESIS/DISSERTATION)	5th out of 15
2005/09/01 - 2008/06/30 Concluded	Science and Technology - Life and earth sciences (Licence) Université Paris-Est Créteil Val de Marne, France "Determination of the precursor cells in the liver fibrogenesis" (THESIS/DISSERTATION)	34th out of 132
2002/09/01 - 2005/06/30 Concluded	Bacharelato em Ciência (Bachelor) Lycée d'Arsonval, France	N.A.

Affiliation

Science

	Category Host institution	Employer
2023/01/01 - Current	Contracted Researcher (Research)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2018/07/15 - 2022/10/14	Contracted Researcher (Research)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

2014/05/01 - 2018/07/14	Researcher (Research)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

2015/09/05 - 2015/09/30	Research Trainee (Research)	Jacobs University Bremen gGmbH, Germany

2010/10/01 - 2013/12/18	Researcher (Research)	Institut de Biochimie et Biophysique Moléculaire et Cellulaire, France

2010/01/01 - 2010/07/31	Research Trainee (Research)	Institut Pasteur, France

2009/02/01 - 2009/04/30	Research Trainee (Research)	École Normale Supérieure Paris-Saclay, France

2008/04/01 - 2008/07/31	Research Trainee (Research)	Institut Mondor de recherche biomédicale, France

Projects

Grant

	Designation	Funders
2023/01/01 - Current	IGNITION: Improving GreeN Innovation for the blue revoluTION: new tools and opportunities for a more sustainable animal farming 101084651 Researcher Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Horizon 2020 Ongoing
2021/07/15 - 2022/07/14	PhotoVac: T3SS and T5SS in Photobacterium damselae subsp. piscicida: from basic studies to vaccine development 165189PRJ Researcher Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2018/07/15 - 2021/07/14	Identification and characterization of virulence factors secreted by Photobacterium damselae piscicida with potential to be used as antigens for vaccination against fish photobacteriosis 151209PRJ Researcher Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2016/05/01 - 2018/07/14	AIP56 as an antigen-delivery platform NORTE-01-0145-FEDER-000012 Post-doc Fellow Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2016/01/01 - 2016/04/30	Host cell factors and molecular mechanisms implicated in intoxication by AIP56, a bacterial toxin targeting NF-kB 148251PRJ Post-doc Fellow Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2015/10/01 - 2015/12/31	Structural determinants required for receptor binding and membrane translocation of AIP56 Incentivo/SAU/LA0002/2014 Post-doc Fellow Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2015/05/15 - 2015/09/30	Studies towards identification of structural determinants required for receptor binding and membrane translocation of AIP56, a Photobacterium damselae subsp. piscicida apoptogenic AB toxin targeting NF-kB 129800PRJ Post-doc Fellow Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2014/05/01 - 2015/05/14	Host-Pathogen Interactions NORTE-07-0124-FEDER-000002 Post-doc Fellow	Fundação para a Ciência e a Tecnologia Concluded
2010/10/01 - 2013/12/19	Dynamique des génomes de bactériophages ANR-10-BLAN-1328 PhD Student Fellow	Agence nationale de la recherche Concluded

Contract

	Designation	Funders
2023/01/01 - Current	IGNITION: Improving GreeN Innovation for the blue revoluTION: new tools and opportunities for a more sustainable animal farming 101084651 Researcher Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Horizon 2020 Ongoing
2021/03/28 - Current	PhotoVac: T3SS and T5SS in Photobacterium damselae subsp. piscicida: from basic studies to vaccine development 165189PRJ Researcher Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2021/01/01 - 2025/12/31	Institute for Research and Innovation in Health LA/P/0070/2020 Researcher Instituto Nacional de Engenharia Biomédica, Portugal Universidade do Porto, Portugal Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2018/07/15 - 2022/07/14	Structural and functional characterization of AB toxins targeting antigen-presenting cells and their application as cancer vaccines 151103PRJ Researcher Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2018/07/15 - 2022/07/14	Identification and characterization of virulence factors secreted by Photobacterium damselae piscicida with potential to be used as antigens for vaccination against fish photobacteriosis 151209PRJ Researcher Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2019/01/01 - 2019/12/31	Instituto de Investigação e Inovação em Saúde UID/BIM/04293/2019 Researcher Instituto Nacional de Engenharia Biomédica, Portugal Universidade do Porto, Portugal Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal Universidade do Porto Instituto de Patologia e Imunologia Molecular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2016/06/01 - 2019/12/01	Host cell factors and molecular mechanisms implicated in intoxication by AIP56, a bacterial toxin targeting NF-kB 148251PRJ Researcher Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2016/05/01 - 2019/04/30	Structured Program on Bioengineered Therapies for Infectious Diseases and Tissue Regeneration NORTE-01-0145-FEDER-000012 Post-doc Fellow Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2013/12/01 - 2015/06/30	Host-Pathogen Interactions NORTE-07-0124-FEDER-000002 Researcher Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010/10/01 - 2013/12/18	French Infrastructure for Integrated Structural Biology ANR-10-INSB-05-01 PhD Student Fellow	Agence nationale de la recherche Concluded
2008/04/01 - 2008/08/31	IMMUNITE INNEE ET FIBROGENESE HEPATIQUES : LE PARADIGME PORTAL ANR-06-PHYS-0022 Other INSERM, France	Agence Nationale pour la Recherche Concluded

Outputs

Publications

Conference paper	Lisboa, Johnny; D. Pinto, Rute; Rodrigues, Inês S.; Pereira, Liliana M.G.; Pereira, Cassilda; Oliveira, Pedro; Pereira, J.B. Pedro; et al. Corresponding author: Lisboa, Johnny. "3D structure of the NF-kB targeting toxin AIP56 and characterization of determinants required for low pH-induced conformational changes, membrane interaction and translocation". Paper presented in IUBMB-FEBS-PABMB 2022 Congress: 'The Biochemistry Global Summit', Lisbon, 2022. Published Celma, Louisa; Philippe, Cuniasse; Durand, Dominique; Lisboa, Johnny; Sanchez, Dyana; Marsin, Stéphanie; Quevillon-Cheruel, Sophie; Radicella, Pablo. "HpDprA domains involved in interaction with DNA". Paper presented in 31st European Crystallographic Meeting, Oviedo, 2018. Published • 10.1107/s2053273318092124 Lisboa, Johnny; Andreani, Jessica; Marsin, Stéphanie; Ochsenbein, Françoise; Guerois, Raphael; Radicella, Pablo; van Tilbeurgh, Herman; Quevillon-Cheruel, Sophie. "Natural genetic transformation in H. pylori: DprA interactome role". Paper presented in 22nd IUBMB, 37th FEBS and 35th SEBBM Congresses, Seville, 2012. Published • 10.1111/j.1742-4658.2010.08705.x
Conference poster	Meireles, Diana; Pombinho R.; Lisboa, Johnny; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. "Biochemical characterization of CadC: a key transcriptional regulator of Listeria monocytogenes virulence factors". Paper presented in 21st International Symposium on Problems of Listeria and Listeriosis (ISOPOL), 2024. Meireles, Diana; Pombinho R.; Lisboa, Johnny; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. "Unveiling the biochemical mechanisms of the Listeria monocytogenes transcriptional repressor CadC". Paper presented in One Health PhD Forum, 2024. Meireles, Diana; Pombinho R.; Lisboa, Johnny; Monteiro, Ricardo; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. "Unravelling signals underlying Listeria monocytogenes cadC regulation". Paper presented in European Congress of Clinical Microbiology and Infectious Diseases, 2024. Meireles, Diana; Pombinho R.; Lisboa, Johnny; Monteiro, Ricardo; Sousa, Sandra; Morais-Cabral, João; Cabanes, Didier. "Uncovering the signals behind the expression of the Listeria monocytogenes regulator CadC". Paper presented in 10th Molecular and Cell Biology Symposium, 2024. Molina-Sorribes, C; Lisboa, Johnny; Sanjuan, E; Fouz, B; do Vale, Ana; Amaro, C. "A new vaccine against the zoonotic pathogen Vibrio vulnificus: First Step". Paper presented in Aquaculture Europe 2023, 2023. Pinto, Alexandre Jorge; Lisboa, Johnny; Barca, Alba V.; Terceti, Mateus S.; Azevedo, Jorge E; Osorio, Carlos R.; dos Santos, Nuno M.S.; do Vale, Ana. "The two-component system RstAB regulates toxin expression in Photobacterium damselae subsp. piscicida". Paper presented in ETOX23 - European Workshop on Bacterial Protein Toxins, 2023. Lisboa, Johnny; Pereira, Cassilda; Bruno Pinheiro; D. Pinto, Rute; Rodrigues, Inês S.; Pereira, Liliana M.G.; Oliveira, Pedro N.; et al. "Three-dimensional structure and mechanisms of membrane interaction and translocation of the NF-kB-targeting toxin AIP56". Paper presented in ETOX23 - European Workshop on Bacterial Protein Toxins, 2023. Bruno Pinheiro; Moura, Ana; Lisboa, Johnny; Azevedo, Jorge E; do Vale, Ana; dos Santos, Nuno M.S.. "Development of tools towards the use of AIP56 as a vehicle for the intracellular delivery of antigens in antigen-presenting cells". Paper presented in ETOX23 - European Workshop on Bacterial Protein Toxins, 2023. José Viveiros; Lisboa, Johnny; Teixeira, Alexandra; Loureiro, Inês; dos Santos, Nuno M.S.; do Vale, Ana. "Recombinant expression and purification of trimeric autotransporter adhesins from Photobacterium damselae subsp. piscicida". Paper presented in IJUP - Encontro de Investigação Jovem, 2023. Bruno Pinheiro; Moura, Ana; Lisboa, Johnny; Azevedo, Jorge E; do Vale, Ana; dos Santos, Nuno M.S.. "Development of tools towards the use of AIP56 as a vehicle for the intracellular delivery of antigens in antigen-presenting cells". Paper presented in XLVIII Annual Meeting of the Portuguese Society for Immunology, 2023. Lisboa, Johnny; Freitas, Inês Lua; do Vale, Ana; dos Santos, Nuno M.S.. "AIP56 and AIP56-like toxins: a family of bacterial toxins that target NF-kB". Paper presented in XLVIII Annual Meeting of the Portuguese Society for Immunology, 2023. Lisboa, Johnny; Freitas, Inês Lua; Pereira, Cassilda; Bruno Pinheiro; D. Pinto, Rute; Inês S. Rodrigues; Pereira, Liliana M.G.; et al. "AIP56 and AIP56-like toxins: a family of bacterial toxins that target NF-kB". Paper presented in 1st ImmunoHUB Annual Meeting, 2023. Bruno Pinheiro; Moura, Ana; Lisboa, Johnny; Azevedo, Jorge E; do Vale, Ana; dos Santos, Nuno M.S.. "Development of tools towards the use of AIP56 as a vehicle for the intracellular delivery of antigens in antigen-presenting cells". Paper presented in 1st ImmunoHUB Annual Meeting, 2023. Bruno Miguel Silva Pinheiro; Moura, Ana; Lisboa, Johnny; Azevedo, Jorge E; do Vale, Ana; dos Santos, Nuno M.S.. "Antigen Delivery by AB toxins". Paper presented in 9th McBiology Symposium, 2023. Lisboa, Johnny; D. Pinto, Rute; Rodrigues, Inês S.; Pereira, Liliana M.G.; Pereira, Cassilda; Oliveira, Pedro; Pereira, J.B. Pedro; et al. "3D structure of the NF-kB targeting toxin AIP56 and characterization of determinants required for low pH-induced conformational changes, membrane interaction and translocation". Paper presented in IUBMB-FEBS-PABMB 2022 Congress: 'The Biochemistry Global Summit', 2022. Pinto, AJ; Lisboa, Johnny; do Vale, Ana; dos Santos, Nuno M.S.. "Role of RstAB in the regulation of toxin expression by Photobacterium damselae subsp. piscicida". Paper presented in 8th Molecular and Cell Biology Symposium, 2022. Lisboa, Johnny; Freitas, Inês Lua; do Vale, Ana; dos Santos, Nuno M.S.. "NiTox: a new Vibrio Nigripulchritudo AB-toxin". Paper presented in ETOX2021 - European Workshop on Bacterial Protein Toxins, 2021. Ana Moura; Lisboa, Johnny; Azevedo, JE; do Vale, Ana; Nuno Miguel Simões dos Santos. "Protein-protein fusion techniques: a new approach to generate AIP56 chimeras for antigen delivery". Paper presented in VI Jornadas da Bioquímica, 2021. Freitas, Inês Lua; Lisboa, Johnny; Ana do vale; Nuno MS dos Santos. "AIP56 and AIP56-related toxins: common receptor, different functions". Paper presented in 7th Molecular and Cell Biology Symposium, 2021. Lisboa, Johnny; Andreia Fernandes; Freitas, Inês Lua; Ana do Vale; Nuno MS dos Santos. "Structural insights on AIP56 and AIP56-related toxins". Paper presented in 8th i3S Annual Meeting, 2019. Inês Marina Soares Loureiro; Alexandra Teixeira; Lisboa, Johnny; A.V. Barca; Osorio, Carlos R.; Fatima Gärtner; Nuno MS dos Santos; Ana do Vale. "Photobacterium deadly duet: uncovering the mechanism of action of a putative binary pore-forming toxin". Paper presented in 8th i3S Annual Meeting, 2019. Bruno Miguel Silva Pinheiro; Inês Lua Araújo Dias Nunes de Freitas (AB10-8825-5AAD); Lisboa, Johnny; Rodrigues, Inês S.; Do Vale, A.; Dos Santos, N.M.S.. "Development of an antigen delivery platform based on AIP56". Paper presented in YES Meeting, 2019. Bruno Miguel Silva Pinheiro; Inês Lua Araújo Dias Nunes de Freitas (AB10-8825-5AAD); Lisboa, Johnny; Rodrigues, Inês S.; Do Vale, A.; Dos Santos, N.M.S.. "Development of an antigen delivery platform based on AIP56". Paper presented in 3RD Symposium on Immunomodulation in Cancer & Regeneration, 2019. Lisboa, Johnny; Fernandes, Andreia; Inês Lua Araújo Dias Nunes de Freitas (AB10-8825-5AAD); Do Vale, A.; Dos Santos, N.M.S.. "Structural insights on AIP56 and AIP56-related toxins". Paper presented in ETOX19 - European Workshop on Bacterial Protein Toxins, 2019. Lisboa, Johnny; Pereira, Cassilda; Ayala, Juan; Terceti, Mateus; Rodrigues, Inês S.; Pereira, J.B. Pedro; Osorio, C.R.; et al. "Characterization of a novel NlpC/P60 endopeptidase of Photobacterium damselae subsp. piscicida secreted by the Type II secretion system". Paper presented in 7th i3S Annual Meeting, 2018. Lisboa, Johnny; Pereira, Cassilda; Ayala, Juan; Terceti, Mateus; Rodrigues, Inês S.; Pereira, J.B. Pedro; Osorio, C.R.; et al. "Characterization of a novel NlpC/P60 endopeptidase of Photobacterium damselae subsp. piscicida secreted by the Type II secretion system". Paper presented in Bacterial Protein Export 2018, 2018. Campos Pinheiro, Jorge Nuno Martins; Lisboa, Johnny; Filipe Freitas Coelho Henriques de Carvalho; Carreaux, Alexis; Nuno MS dos Santos; Sandra Sousa; Didier Cabanes. "LarS controls the expression of the Agr system of Listeria monocytogenes and mediates virulence". Paper presented in 7th Congress of European Microbiologists - FEMS 2017, 2017. Lisboa, Johnny; Rodrigues, Inês S.; Benz, Roland; Do Vale, A.; Dos Santos, N.M.S.. "Structural determinants required for pH-induced unfolding and pore formation in AIP56". Paper presented in ETOX18 – European Workshop on Bacterial Protein Toxins, 2017. Rodrigues, Inês S.; Pereira, Cassilda; Pereira, Liliana M.G.; Lisboa, Johnny; Dos Santos, N.M.S.; Do Vale, A.. "Host factors involved in AIP56 intoxication: the role of protein chaperones and redox systems". Paper presented in ETOX18 – European Workshop on Bacterial Protein Toxins, 2017. Lisboa, Johnny; Rodrigues, Inês S.; Pereira, Liliana M.G.; Pinto, Rute D.; Peres, J.; Do Vale, A.; Dos Santos, N.M.S.. "AIP56: a short-trip AB toxin with a peculiar translocation domain". Paper presented in I3S Retreat- 5th Annual Meeting, 2016. Rodrigues, Inês S.; Pereira, Liliana M.G.; Pinto, Rute D.; Lisboa, Johnny; Dos Santos, N.M.S.; Do Vale, A.. "Involvement of Hsp90 and PPIases in AIP56 intoxication". Paper presented in I3S Retreat- 5th Annual Meeting, 2016. Pereira, Liliana M.G.; Pinto, Rute D.; Peres, J.; Rodrigues, Inês S.; Lisboa, Johnny; Dos Santos, N.M.S.; Do Vale, A.. "Involvement of Hsp90 and PPIases in AIP56 intoxication". Paper presented in ETOX17 - 17th European Workshop on Bacterial Protein Toxins, 2015. Peres, J.; Rodrigues, Inês S.; Pereira, Liliana M.G.; Pinto, Rute D.; Lisboa, Johnny; Do Vale, A.; Dos Santos, N.M.S.. "AIP56: a short-trip AB toxin with a peculiar translocation domain". Paper presented in ETOX17 - 17th European Workshop on Bacterial Protein Toxins, 2015. Lisboa, Johnny; Ochsenbein, Françoise; Durand, Dominique; van Tilbeurgh, Herman; Guerois, Raphael; Quevillon-Cheruel, Sophie. "The natural transformation in H. pylori: structural study of DprA interactome". Paper presented in AFC Meeting, 2013. Lisboa, Johnny; Ochsenbein, Françoise; Durand, Dominique; van Tilbeurgh, Herman; Guerois, Raphael; Quevillon-Cheruel, Sophie. "The natural transformation in H. pylori: structural study of DprA interactome". Paper presented in The 10th Meeting of 3R, 2013. Lisboa, Johnny; Ochsenbein, Françoise; Durand, Dominique; van Tilbeurgh, Herman; Guerois, Raphael; Quevillon-Cheruel, Sophie. "The natural transformation in H. pylori: structural study of DprA interactome". Paper presented in The 4th Microbial Genome Maintenance Meeting: Transformation and DNA Repair, 2013. Lisboa, Johnny; Andreani, Jessica; Prado-Acosta, Mariano; Marsin, Stéphanie; Ochsenbein, Françoise; Guerois, Raphael; Durand, Dominique; et al. "DprA role in the natural genetic transformation in H. pylori". Paper presented in The 4th EMBO Meeting, 2012. Lisboa, Johnny; Andreani, Jessica; Prado-Acosta, Mariano; Marsin, Stéphanie; Ochsenbein, Françoise; Guerois, Raphael; Durand, Dominique; et al. "DprA role in the natural genetic transformation in H. pylori". Paper presented in From Single Molecules to Systems Biology - 22nd IUBMB & 37th FEBS Congress, 2012. Lisboa, Johnny; Andreani, Jessica; Marsin, Stéphanie; Ochsenbein, Françoise; Guerois, Raphael; Durand, Dominique; van Tilbeurgh, Herman; Radicella, Pablo; Quevillon-Cheruel, Sophie. "Natural genetic transformation in H. pylori: research for functional and structural peculiarities of DprA". Paper presented in 12th Meeting of the Doctoral School, Therapeutic Innovation, 2012. Lisboa, Johnny; Andreani, Jessica; Marsin, Stéphanie; Ochsenbein, Françoise; Guerois, Raphael; Durand, Dominique; van Tilbeurgh, Herman; Radicella, Pablo; Quevillon-Cheruel, Sophie. "Natural genetic transformation in H. pylori: research for functional and structural peculiarities of DprA". Paper presented in GTBio 2012, 2012.
Journal article	Morais, Mariana; Dias, Francisca; Figueiredo, Patrícia; Tavares, Inês; Escudeiro, Carla; Teixeira, Manuel R.; Teixeira, Alexandra; et al. "Glucose-functionalized Silver Nanoparticles (G-AgNPs) uptake by caveolae-dependent endocytosis is responsible for their selective effect towards Castration Resistant Prostate Cancer". International Journal of Nanomedicine (2024): Accepted Lisboa, Johnny; Pereira, Cassilda; Pinto, Rute D.; Rodrigues, Inês S.; Pereira, Liliana M. G.; Pinheiro, Bruno; Oliveira, Pedro; et al. Corresponding author: Lisboa, Johnny. "Unconventional structure and mechanisms for membrane interaction and translocation of the NF-kB-targeting toxin AIP56". Nature Communications 14 1 (2023): http://dx.doi.org/10.1038/s41467-023-43054-z. 10.1038/s41467-023-43054-z Teixeira, Alexandra; Loureiro, Inês; Lisboa, Johnny; Oliveira, Pedro N.; Azevedo, Jorge E.; dos Santos, Nuno M. S.; do Vale, Ana. "Characterization and Vaccine Potential of Outer Membrane Vesicles from Photobacterium damselae subsp. piscicida". International Journal of Molecular Sciences 24 6 (2023): 5138. http://dx.doi.org/10.3390/ijms24065138. Published • 10.3390/ijms24065138 Freitas, Inês Lua; Teixeira, Alexandra; Loureiro, Inês; Lisboa, Johnny; Saraiva, Aurélia; dos Santos, Nuno Miguel Simões; do Vale, Ana. "Susceptibility of Sea Bream (Sparus aurata) to AIP56, an AB-Type Toxin Secreted by Photobacterium damselae subsp. piscicida". Toxins 14 2 (2022): 119. http://dx.doi.org/10.3390/toxins14020119. Published • 10.3390/toxins14020119 Lisboa, Johnny; Pereira, Cassilda; Rifflet, Aline; Ayala, Juan; Terceti, Mateus S.; Barca, Alba V.; Rodrigues, Inês; et al. Corresponding author: Lisboa, Johnny. "A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida Cleaves the Peptidoglycan of Potentially Competing Bacteria". mSphere 6 1 (2021): http://dx.doi.org/10.1128/msphere.00736-20. Published • 10.1128/msphere.00736-20 Ithurbide, Solenne; Coste, Geneviève; Lisboa, Johnny; Eugénie, Nicolas; Bentchikou, Esma; Bouthier de la Tour, Claire; Liger, Dominique; et al. "Natural Transformation in Deinococcus radiodurans: A Genetic Analysis Reveals the Major Roles of DprA, DdrB, RecA, RecF, and RecO Proteins". Frontiers in Microbiology 11 (2020): http://dx.doi.org/10.3389/fmicb.2020.01253. Published • 10.3389/fmicb.2020.01253 Rodrigues, Inês S.; pereira, Liliana M. G.; Lisboa, Johnny; Pereira, Cassilda; Oliveira, Pedro; Santos, Nuno M. S. dos; Vale, Ana do. "Author Correction: Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida". Scientific Reports 10 1 (2020): http://dx.doi.org/10.1038/s41598-020-58152-x. Published • 10.1038/s41598-020-58152-x Inês S. Rodrigues; Liliana M. G. Pereira; Johnny Lisboa; Cassilda Pereira; Pedro Oliveira; Nuno M. S. dos Santos; Ana do Vale. "Involvement of Hsp90 and cyclophilins in intoxication by AIP56, a metalloprotease toxin from Photobacterium damselae subsp. piscicida". Scientific Reports 9 1 (2019): https://doi.org/10.1038/s41598-019-45240-w. Published • 10.1038/s41598-019-45240-w Pereira, Cassilda; Rodrigues, Inês S.; Pereira, Liliana M.G.; Lisboa, Johnny; Pinto, Rute D.; Araújo, Leonor; Oliveira, Pedro; et al. "Role of AIP56 disulphide bond and its reduction by cytosolic redox systems for efficient intoxication". Cellular Microbiology 22 1 (2019): http://dx.doi.org/10.1111/cmi.13109. Published • 10.1111/cmi.13109 Terceti, Mateus S.; Vences, Ana; Matanza, Xosé M.; Barca, Alba V.; Noia, Manuel; Lisboa, Johnny; dos Santos, Nuno M. S.; do Vale, Ana; Osorio, Carlos R.. "The RstAB System Impacts Virulence, Motility, Cell Morphology, Penicillin Tolerance and Production of Type II Secretion System-Dependent Factors in the Fish and Human Pathogen Photobacterium damselae subsp. damselae". Frontiers in Microbiology 10 (2019): http://dx.doi.org/10.3389/fmicb.2019.00897. Published • 10.3389/fmicb.2019.00897 Lisboa J; Celma L; Sanchez D; Marquis M; Andreani J; Guérois R; Ochsenbein F; et al. "The C-terminal domain of HpDprA is a DNA-binding Winged Helix domain that does not bind double-stranded DNA.". The FEBS journal (2019): http://europepmc.org/abstract/med/30771270. Published • 10.1111/febs.14788 Pinheiro, J.; Lisboa, J.; Pombinho, R.; Carvalho, F.; Carreaux, A.; Brito, C.; Pöntinen, A.; et al. "MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence". Nucleic acids research 46 18 (2018): 9338-9352. http://www.scopus.com/inward/record.url?eid=2-s2.0-85054896319&partnerID=MN8TOARS. Published • 10.1093/nar/gky624 Devigne, A.; Guérin, P.; Lisboa, J.; Quevillon-Cheruel, S.; Armengaud, J.; Sommer, S.; de la Tour, C.B.; Servant, P.. "PprA protein is involved in chromosome segregation via its physical and functional interaction with DNA gyrase in irradiated Deinococcus radiodurans bacteria". mSphere 1 1 (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-85019363618&partnerID=MN8TOARS. Published • 10.1128/mSphere.00036-15 Serre, Marie-Claude; El Arnaout, Toufic; Brooks, Mark A.; Durand, Dominique; Lisboa, Johnny; Lazar, Noureddine; Raynal, Bertrand; van Tilbeurgh, Herman; Quevillon-Cheruel, Sophie. "Correction: The Carboxy-Terminal aN Helix of the Archaeal XerA Tyrosine Recombinase Is a Molecular Switch to Control Site-Specific Recombination". PLoS ONE 9 1 (2014): http://dx.doi.org/10.1371/annotation/ad556340-dea0-40c9-b90c-43198d427095. Published • 10.1371/annotation/ad556340-dea0-40c9-b90c-43198d427095 Lisboa, J.; Andreani, J.; Sanchez, D.; Boudes, M.; Collinet, B.; Liger, D.; Tilbeurgh, H. v.; Guerois, R.; Quevillon-Cheruel, S.. "Molecular determinants of the DprA-RecA interaction for nucleation on ssDNA". Nucleic Acids Research 42 11 (2014): 7395-7408. https://doi.org/10.1093/nar/gku349. Published • 10.1093/nar/gku349 Serre, Marie-Claude; El Arnaout, Toufic; Brooks, Mark A.; Durand, Dominique; Lisboa, Johnny; Lazar, Noureddine; Raynal, Bertrand; et al. "The Carboxy-Terminal aN Helix of the Archaeal XerA Tyrosine Recombinase Is a Molecular Switch to Control Site-Specific Recombination". PLoS ONE 8 5 (2013): e63010-e63010. https://doi.org/10.1371/journal.pone.0063010. Published • 10.1371/journal.pone.0063010 Quevillon-Cheruel, S.; Campo, N.; Mirouze, N.; Mortier-Barriere, I.; Brooks, M. A.; Boudes, M.; Durand, D.; et al. "Structure-function analysis of pneumococcal DprA protein reveals that dimerization is crucial for loading RecA recombinase onto DNA during transformation". Proceedings of the National Academy of Sciences 109 37 (2012): E2466-E2475. https://doi.org/10.1073/pnas.1205638109. Published • 10.1073/pnas.1205638109 Durand, Dominique; Li de la Sierra-Gallay, Ines; Brooks, Mark A.; Thompson, Andrew W.; Lazar, Noureddine; Lisboa, Johnny; van Tilbeurgh, Herman; Quevillon-Cheruel, Sophie. "Expression, purification and preliminary structural analysis of Escherichia coli MatP in complex with the matS DNA site". Acta Crystallographica Section F Structural Biology and Crystallization Communications 68 6 (2012): 638-643. https://doi.org/10.1107/S1744309112011062. Published • 10.1107/S1744309112011062
Online resource	Lisboa, Johnny; Pereira, P.J.B.; Dos Santos, N.M.S.. Three-dimensional structure of AIP56, a short-trip single chain AB toxin from Photobacterium damselae subsp. piscicida [7ZPF]. 2023. https://www.rcsb.org/structure/7ZPF. https://doi.org/10.2210/pdb7ZPF/pdb Lisboa, Johnny; Durand, Dominique. SASDNW6 – AIP56, an AB-toxin from Photobacterium damselae subsp. piscicida. 2023. https://www.sasbdb.org/data/SASDNW6/. Lisboa, Johnny; Pereira, P.J.B.; Dos Santos, N.M.S.. Insights into a novel NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida [6SQX]. 2020. https://www.rcsb.org/structure/6SQX. https://doi.org/10.2210/pdb6SQX/pdb Lisboa, Johnny; Celma, Louisa; Sanchez, D.; Marquis, M. ; Andreani, Jessica; Guerois, Raphael; Ochsenbein, Françoise; et al. The CTD of HpDprA, a DNA binding Winged Helix domain which do not bind dsDNA [6GW7]. 2019. https://www.rcsb.org/structure/6GW7. https://doi.org/10.2210/pdb6GW7/pdb Lisboa, Johnny; Dos Santos, N.M.S.. Cyclophilin A [6FK1]. 2019. https://www.rcsb.org/structure/6FK1. https://doi.org/10.2210/pdb6FK1/pdb Campos Pinheiro, J.; Lisboa, Johnny; Carvalho, F.; Carreaux, A.; Dos Santos, N.M.S.; Morais-Cabral, J.H.; Sousa, Sandra; Cabanes, Didier. Lar controls the expression of the Listeria monocytogenes agr system and mediates virulence [6EP3]. 2018. https://www.rcsb.org/structure/6EP3. https://doi.org/10.2210/pdb6EP3/pdb Lisboa, Johnny; Quevillon-Cheruel, S.. Structure of HpDprA at 1.9 Angstroms resolution [5MLL]. 2017. https://www.rcsb.org/structure/5MLL. https://doi.org/10.2210/pdb5MLL/pdb Brooks, M.A.; El Arnaout, T.; Durand, D.; Lisboa, Johnny; Lazar, N.; Raynal, B.; van Tilbeurgh, H.; Serre, M.; Quevillon-Cheruel, S.. The structure of a dimeric Xer recombinase from archaea [4A8E]. 2012. https://www.rcsb.org/structure/4A8E. https://doi.org/10.2210/pdb4A8E/pdb
Preprint	Lisboa, Johnny; Pereira, Cassilda; Pinto, Rute D.; Rodrigues, Inês S.; Pereira, Liliana M. G.; Pinheiro, Bruno; Oliveira, Pedro; et al. "Structural and functional characterization of the NF-kB-targeting toxin AIP56 from Photobacterium damselae subsp. piscicida reveals a novel mechanism for membrane interaction and translocation". 2023. http://dx.doi.org/10.1101/2023.05.04.539443. 10.1101/2023.05.04.539443 Johnny Lisboa; Cassilda Pereira; Aline Rifflet; Juan Ayala; Mateus S. Terceti; Alba V. Barca; Inês Rodrigues; et al. "A secreted NlpC/P60 endopeptidase from Photobacterium damselae subsp. piscicida cleaves the peptidoglycan of potentially competing bacteria". 2020. https://doi.org/10.1101/2020.06.30.181511. 10.1101/2020.06.30.181511

Activities

Oral presentation

	Presentation title	Event name Host (Event location)
2023/09/11	The two-component system RstAB positively regulates the expression of secreted virulence factors in Photobacterium damselae subsp. piscicida (*Ana do Vale as presenting author)	21st International Conference on Diseases of Fish and Shellfish (Aberdeen)
2023/03/17	PBT: a novel binary toxin from the fish pathogen Photobacterium damselae subsp. piscicida that targets fish, mouse and human macrophages (Best Oral Presentation Award, *Inês Loureiro as presenting author)	I ImmunoHUB Annual Meeting - Infection & Immunity i3S - Instituto de Investigação e Inovação em Saúde (Porto, Portugal)
2022/11/30	AIP56, a single chain AB toxin with a unique structural organization and translocation mechanism	MicroHub meeting CIIMAR (Porto, Portugal)
2022/11/04	Photobacterium deadly duet: activity of a novel virulence-associated binary toxin. (*Inês Loureiro as presenting author)	Virtual International Aquaculture and Fisheries Conference
2022/05/04	The two-component system RstAB regulates expression of virulence factors in Photobacterium damselae subsp. piscicida. (*Alexandre Pinto as presenting author)	IJUP - Encontro de Investigação Jovem (Porto, Portugal)
2022/05/02	AIP56 and AIP56-like toxins: a family of bacterial toxins that target NF-kB. (*Inês Lua Freitas as presenting author)	5th European NF-kappaB Subunit Workshop (Porto, Portugal)
2021/09/20	Structural and functional characterization of a PirAB-like binary toxin from Photobacterium damselae subsp. piscicida. (*Ana do Vale as presenting author)	Virtual 20th International Conference on Diseases of Fish and Shellfish European Association of Fish Pathologists (Virtual)
2021/09/20	Structural and functional characterization of NiTox: a new AB-toxin from Vibrio Nigripulchritudo lethal to shrimp	Virtual 20th International Conference on Diseases of Fish and Shellfish European Association of Fish Pathologists (Virtual)
2021/06/28	Photobacterium deadly duet: structure and activity of a novel virulence-associated binary toxin. (*Ana do Vale as presenting author)	ETOX2021 - European Workshop on Bacterial Protein Toxins Max Planck Institute of Molecular Physiology Dortmund, Germany (Online, Germany)
2021/06/14	AIP56, a single chain AB toxin with a unique structural organization	7th International Iberian Biophysics Congress University of Coimbra (Porto, Portugal)
2021/05/05	Biotechnological application of AIP56, an AB-toxin from Photobacterium damselae subsp. piscicida. (*Ana Moura as presenting author)	IJUP - Encontro de Investigação Jovem University of Porto (Porto, Portugal)
2019/06/22	Structural insights on AIP56 and AIP56-related toxins. (*Nuno M.S. dos Santos as presenting author)	ETOX19 - European Workshop on Bacterial Protein Toxins (Davos, Switzerland)
2015/09/29	AIP56, an NF-kB cleaving toxin from Photobacterium damselae piscicida (Phdp)	MOLIFE RESEARCH SEMINAR Research II - Lecture Hall (Bremen, Germany)
2013	The natural transformation in H. pylori: structural study of DprA interactome	13th Meeting of the Doctoral School, Therapeutic Innovation (França, France)
2013	The natural transformation: DprA interactome	Frisbi Meeting (French Infrastructure for Integrated Structural Biology) (CEA Saclay, France)
2013	Conference in a licence class (equivalent to a Bachelor's degree)	(University Paris VI, France)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2021 - Current	Characterization of a novel family of AB toxins with potential to be used as antigen delivery vehicles Co-supervisor of Inês Lua Freitas	Molecular and Cell Biology (PhD) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022 - 2023	Recombinant expression and purification of trimeric autotransporter adhesins from Photobacterium damselae subsp. piscicida Co-supervisor of José António Gouveia Viveiros	Degree in Biochemistry (Degree) Universidade do Porto Faculdade de Ciências, Portugal Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021 - 2022	Trimeric autotransporter adhesins (TAAs) from Photobacterium damselae subsp. piscicida: investigation of biological functions and recombinant production to use as antigens Co-supervisor of Gabriela Alves Afonso	Genética Molecular Comparativa e Tecnológica (Master) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal Universidade de Trás-os-Montes e Alto Douro, Portugal
2021 - 2022	Studies on the regulation of Photobacterium damselae subsp. piscicida toxins by the two-component system RstAB Co-supervisor of Alexandre Pinto	Master in Biochemistry (Master) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2021 - 2021	The understanding of AIP56-like proteins Supervisor of Clothilde Colart	Magistère Européen de Génétique (Master) Université de Paris, France
2020 - 2021	AIP56 and AIP56-related toxins as antigen delivery platforms Co-supervisor of Ana Moura	Master in Biochemistry (Master) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2019 - 2020	Studies on the regulation of expression of AIP56 by RstA of Photobacterium damselae subsp. piscicida Co-supervisor of Alexandre Pinto	Degree in Biochemistry (Degree) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017 - 2018	AIP56, an AB toxin from the fish pathogenic bacterium Photobacterium damselae subsp. piscicida, as a possible drug and/or antigen delivery system for leishmaniasis Co-supervisor of Laura Dirkx	Master in Biomedical Sciences (Master) Universiteit Antwerpen, Belgium Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017 - 2018	AIP56 as an antigen delivery platform: OVA peptide-AIP56 fusion as a proof of concept Co-supervisor of Ana Manuela Canha Borges	Degree in Biology (Degree) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2017 - 2018	Comparative analysis of proteins secreted by different strains of Photobacterium damselae subsp. piscicida Co-supervisor of Patricia Martins Ribeiro	Degree in Biotechnology (Degree) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal Instituto Politécnico de Coimbra Escola Superior Agrária de Coimbra, Portugal
2016 - 2017	Translocation mechanism and biotechnological application of AIP56 Co-supervisor of Diogo Moreira Filipe	Degree in Biology (Degree) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2016 - 2017	Study towards the Use of AIP56 as an Antigen Delivery Platform Co-supervisor of Sandra Lindner	Degree in Biology (Degree) The Ostbayerische Technische Hochschule Amberg-Weiden (OTH – Technical University of Applied Sciences), Germany Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2012 - 2013	Structural and functional study of ComD-ComE, the two-component signal-transduction system that controls competence of Streptococcus pneumoniae Co-supervisor of Dyana Sanchez	Master in Biochemistry (Master) Institut de Biochimie et Biophysique Moléculaire et Cellulaire, France
2010/03 - 2010/06	Construction of new secretion vectors for Escherichia coli recombinants proteins Co-supervisor of Cédric Radigue	Degree in Public Health at the University of Paris XI (Degree) Institut Pasteur, France

Event organisation

	Event name Type of event (Role)	Institution / Organization
2015/06/20 - 2015/06/24	ETOX17 - 17th European Workshop on Bacterial Protein Toxins (2015/06/20 - 2015/06/24) Congress (Member of the Organising Committee)	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Event participation

Jury of academic degree

	Topic Role	Candidate name (Type of degree) Institution / Organization
2016/07/20	Crystallization of New Therapeutic Targets Against Mycobacteria (Thesis) Main arguer	Hélio Martins Gil (Other) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal Universidade do Porto Faculdade de Ciências, Portugal

Ad Hoc journal article review

	Journal title (ISSN)	Publisher
2024 - Current	International Journal of Molecular Sciences (1422-0067)	MDPI
2022 - Current	Journal of Bacteriology (0021-9193)	American Society for Microbiology (ASM)
2021 - Current	Scientific Reports (2045-2322)	Nature Portfolio
2017 - Current	Molecular Microbiology (0950-382X)	Wiley

Association member

	Society Organization name	Role
2020/03 - Current	Portuguese Biochemical Society (SPB)	Member
2020/01/01 - Current	American Society for Microbiology (ASM)	Postdoctoral Membership
2012 - 2013	French Society for Biochemistry and Molecular Biology (SFBBM)	Member
2012 - 2013	French Crystallography Association (AFC)	Member

Committee member

	Activity description Role	Institution / Organization
2011 - 2013	Docs/Postdocs representant in the council of the IBBMC (Orsay, France) President / Vice-president	Institut de Biochimie et Biophysique Moléculaire et Cellulaire, France

Conference scientific committee

	Conference name	Conference host
2021/11/30 - 2021/11/30	4th PhDay: Break borders and build opportunities	i3S PhD students

Journal scientific committee

	Journal title (ISSN)	Publisher
2024 - Current	International Journal of Molecular Sciences (1422-0067)	MDPI

Distinctions

Award

2024	Top Rated Poster in the 34th Congress of the European Society of Clinical Microbiology and Infectious Diseases (*Diana Meireles as presenting author)
2023	Best Oral Communication in ImmunoHUB Annual Meeting - Infection & Immunity. (*Inês Loureiro as presenting author) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022	Best Poster in 8th Molecular and Cell Biology Symposium. (*Alexandre Pinto as presenting author) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022	"Susceptibility of Sea Bream (Sparus aurata) to AIP56, an AB-Type Toxin Secreted by Photobacterium damselae subsp. Piscicida" selected as an Editor's Choice Article
2021	Best Poster in 7th Molecular and Cell Biology Symposium. (*Inês Lua Freitas as presenting author) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal

Other distinction

2023	Junior Researcher Contract (Bio/Horizon/i3S/0211/2022)
2021	Junior Researcher Contract (PTDC/CVT-CVT/7177/2020) Fundação para a Ciência e a Tecnologia, Portugal
2018	Junior Researcher Contract (PTDC/BIA-MIC/29910/2017) Fundação para a Ciência e a Tecnologia, Portugal
2016	Post-Doc Fellowship (NORTE-01-0145-FEDER-000012) Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2015	Post-Doc Fellowship (FCOMP-01-0124-FEDER-028364-PTDC/BIA-MIC/3463/2012) Fundação para a Ciência e a Tecnologia, Portugal
2015	Post-Doc Fellowship (Incentivo/SAU/LA0002/2014) Fundação para a Ciência e a Tecnologia, Portugal
2014	Post-Doc Fellowship (NORTE-07-0124-FEDER-000002)
2013	JP Ebel
2013	AFC Grant The French Crystallography Association (AFC), France
2012	Travel Grant IUBMB/FEBS/SEBBM, Portugal
2010	Doctoral School Grant Doctoral School, ED425, Portugal
2010	PhD Fellowship

	Activity description Type of event	Event name Institution / Organization
2014 - Current	Active roles in lab management and logistics. Sole responsibility for lab ordering and stocking system. Assistant in lab rota and maintenance management. Assistant in lab budgeting and expenses managing. Other	Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2022/07/09 - 2022/07/14	IUBMB-FEBS-PABMB 2022 Congress: 'The Biochemistry Global Summit'_Poster Presentation Congress	IUBMB-FEBS-PABMB 2022 Congress: 'The Biochemistry Global Summit'
2021/09/20 - 2021/09/23	Virtual 20th International Conference on Diseases of Fish and Shellfish_Oral Communication Conference	Virtual 20th International Conference on Diseases of Fish and Shellfish
2021/06/28 - 2021/07/01	ETOX2021 - European Workshop on Bacterial Protein Toxins_Poster Presentation Workshop	ETOX2021 - European Workshop on Bacterial Protein Toxins
2021/06/14 - 2021/06/16	7th International Iberian Biophysics Congress_Oral Communication Congress	7th International Iberian Biophysics Congress
2019 - 2019	8th i3S Annual Meeting_Poster Presentation Meeting	8th i3S Annual Meeting
2018 - 2018	7th i3S Annual Meeting_Poster Presentation Meeting	7th i3S Annual Meeting
2017 - 2017	ETOX18 – European Workshop on Bacterial Protein Toxins_Poster Presentation Workshop	ETOX18 – European Workshop on Bacterial Protein Toxins
2016 - 2016	I3S Retreat- 5th Annual Meeting_Poster Presentation Meeting	I3S Retreat- 5th Annual Meeting
2015 - 2015	ETOX17 - 17th European Workshop on Bacterial Protein Toxins_Poster Presentation Workshop	ETOX17 - 17th European Workshop on Bacterial Protein Toxins
2015 - 2015	MOLIFE RESEARCH SEMINAR_Oral Communication Seminar	MOLIFE RESEARCH SEMINAR Jacobs University Bremen gGmbH, Germany
2013 - 2013	The 4th Microbial Genome Maintenance Meeting: Transformation and DNA Repair_Poster Presentation Conference	The 4th Microbial Genome Maintenance Meeting: Transformation and DNA Repair
2013 - 2013	The 10th Meeting of 3R_Poster Presentation Meeting	The 10th Meeting of 3R
2013 - 2013	AFC Meeting_Poster Presentation Meeting	AFC Meeting
2013 - 2013	Frisbi Meeting (French Infrastructure for Integrated Structural Biology)_Oral Communication Meeting	Frisbi Meeting (French Infrastructure for Integrated Structural Biology)
2013 - 2013	13th Meeting of the Doctoral School, Therapeutic Innovation_Oral Communication Meeting	13th Meeting of the Doctoral School, Therapeutic Innovation
2012 - 2012	Congress of the French biocrystallography group (GTBio)_Poster Presentation Congress	Congress of the French biocrystallography group (GTBio)
2012 - 2012	12th Meeting of the Doctoral School, Therapeutic Innovation_Poster Presentation Meeting	12th Meeting of the Doctoral School, Therapeutic Innovation
2012 - 2012	From Single Molecules to Systems Biology - 22nd IUBMB & 37th FEBS Congress_Poster Presentation Congress	From Single Molecules to Systems Biology - 22nd IUBMB & 37th FEBS Congress
2012 - 2012	The 4th EMBO Meeting_Poster Presentation Meeting	The 4th EMBO Meeting