???global.info.a_carregar???
Nuno Miguel da Rocha Guimarães obtained his degree in Biochemistry in 2004, at the Faculty of Sciences of University of Lisbon. In 2006, he won a PhD grant from the Foundation of Science and Technology (FCT), with the work entitled “On the routes of Helicobacter pylori transmission among the humans”, being supervised by Dr. Maria João Vieira from University of Minho and Dr. Céu Figueiredo from Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP). In 2011, he completed his PhD in Chemical and Biological Engineering. He was a Pos-Doctoral Researcher at LEPABE from January of 2012 until December 2017, after being selected in an extremely competitive national contest from FCT (with a remark of 4.72/5.00) with a project entitled “Anti-bacterial DNA mimics as a novel technique for Helicobacter pylori treatment”. His research has been mainly focused on the design and synthesis of nucleic acid mimics (NAMs) oligonucleotides for detection and/or growth inhibition of microorganisms, and their application in conditions mimicking the human body. He spent 2 months in Southern Denmark University working under supervision of Dr. Jesper Wengel, the inventor of LNA and 2’Ome nucleic acid mimics, where he improved his skills and knowledge on the design and synthesis of oligonucleotides using these 2 NAMs. At LEPABE, he was involved in the initial works regarding the optimization and adaptation of the FISH method in order to work against different bacteria in conditions similar to the human body. More recently, together with the previous research topic, his research strategy also includes the development of delivery mechanisms of this nucleic acids into the microorganisms. Currently, he is a Doctoral Researcher at LEPABE since June of 2018. From the point of view of the research activity, he has authored or co-authored 21 scientific papers in international journals indexed by Science Citation Index or Scopus, with 318 citations (H-index of 10). He has also published 5 book chapters and several abstracts in international conference proceedings. His work has been presented in several national and international conferences. He was also co-author of three patents. He participated in different competitive national and international research projects as EVK1-CT-2002-00108, SAFER - Surveillance and control of microbiological stability in drinking water distribution networks; PTDC/BIA-MIC/108811/2008, HELIWATER - Water and associated biofilms as a possible vector for Helicobacter pylori transmission among the human population; PTDC/DTP-PIC/4562/2014|16678, “Development of a novel color-coded RING FISH method: an innovative tool for the detection of antibiotic resistant clinical pathogens”, “uFISH - Integration of fluorescence in situ hybridization with microfluidics for the quasi real-time detection of food pathogens”, “ColorISH - Development of a new colorimetric method for the rapid detection of Legionella spp. in water sources”, "NanoSAURUS - Nanotech-double functionalized particles to transport Nucleic Acid Mimics against Staphylococcus Aureus" and "NAMSal - Nucleic acid mimics as a new tool against salmonellosis in the poultry industry" all founded by Fundação para a Ciência e Tecnologia. He also participate in the project obtained through an international call, H2020-WIDESPREAD-2016-2017, Strengthening the Research Area of Delivery of Nucleic acid mimics (NAMS) into bacteria to fight the antibiotic crisis. In his curriculum, Nuno Guimarães has co-supervised 1 PhD and 1 MSc thesis and has been co-advisor in 3 PhD thesis, 1 MSc thesis and 5 end of course Erasmus projects, and has been invited as external evaluator of 2 Master thesis. He has also participated in the organization of national and international scientific meetings and workshops. Since 2016, he has been teaching at FEUP in the Integrated Master in Bioengineering (MIB), which has been awarded by the international EUR.
Identification

Personal identification

Full name
Nuno Miguel Rocha Guimarães

Citation names

  • Guimarães, Nuno

Author identifiers

Ciência ID
4113-0939-1967
ORCID iD
0000-0002-7623-3877
Scopus Author Id
1045817

Email addresses

  • nguimaraes@fe.up.pt (Professional)

Websites

Knowledge fields

  • Medical and Health Sciences - Medical Biotechnology - Health-Related Biotechnology
  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions

Languages

Language Speaking Reading Writing Listening Peer-review
English Proficiency (C2) Proficiency (C2) Advanced (C1) Proficiency (C2) Advanced (C1)
French Beginner (A1) Upper intermediate (B2) Beginner (A1) Beginner (A1) Beginner (A1)
Spanish; Castilian Intermediate (B1) Upper intermediate (B2) Elementary (A2) Upper intermediate (B2) Intermediate (B1)
Education
Degree Classification
2006 - 2011
Concluded
Engenharia Química e Biológica (Doutoramento)
Major in Especialidade: Química Física
Universidade do Minho, Portugal
"On the Routes of Helicobacter Pylori Transmission Among the Humans" (THESIS/DISSERTATION)
Aprovado (Classificação máxima)
2004
Concluded
Bioquímica (Licenciatura)
Universidade de Lisboa Faculdade de Ciências, Portugal
"Influência de alguns polimorfismos do eixo renina-angiotensina em patologias cardiovasculares na população portuguesa " (THESIS/DISSERTATION)
15 valores
Affiliation

Science

Category
Host institution
Employer
2024/08/09 - Current Contracted Researcher (Research) Universidade do Porto Faculdade de Engenharia, Portugal
2018/06/08 - 2024/06/07 Contracted Researcher (Research) Universidade do Porto Faculdade de Engenharia, Portugal
2018/01/03 - 2018/06/07 Postdoc (Research) Universidade do Porto Faculdade de Engenharia, Portugal
Universidade do Porto Faculdade de Engenharia, Portugal
2012/01/02 - 2017/12/31 Postdoc (Research) Universidade do Porto Faculdade de Engenharia, Portugal
Universidade do Porto Faculdade de Engenharia, Portugal
(...)

Teaching in Higher Education

Category
Host institution
Employer
2016/09/01 - 2022/02/28 Invited Assistant Professor (University Teacher) Universidade do Porto, Portugal
Universidade do Porto Faculdade de Engenharia, Portugal
Projects

Grant

Designation Funders
2012/01/01 - 2017/12/31 ANTI-BACTERIAL DNA MIMICS AS A NOVEL TECHNIQUE FOR HELICOBACTER PYLORI TREATMENT
info:eu-repo/grantAgreement/FCT/SFRH/SFRH2%FBPD2%F788462%F2011/PT
Universidade do Porto Faculdade de Engenharia, Portugal

Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2012/01/01 - 2017/12/31 ANTI-BACTERIAL DNA MIMICS AS A NOVEL TECHNIQUE FOR HELICOBACTER PYLORI TREATMENT
SFRH/BPD/78846/2011
Fundação para a Ciência e a Tecnologia
Concluded
2006/01/01 - 2009/12/31 ON THE ROUTES OF HELICOBACTER PYLORI TRANSMISSION AMONG THE HUMANS
SFRH/BD/24579/2005
Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2023/03/01 - 2026/02/28 NAMSal - Mímicos de ácidos nucleicos como uma nova ferramenta para o combate de salmonelose na indústria pecuária
2022.07654.PTDC
Researcher
Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2021/01/01 - 2025/12/31 ALICE - Associate Laboratory in Chemical Engineering
LA/P/0045/2020
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal

Universidade do Porto Centro de Estudos de Fenómenos de Transporte, Portugal

Universidade do Porto Laboratório de Processos de Separação e Reacção Laboratório de Catálise e Materiais, Portugal

Instituto Politécnico de Leiria, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2022/01/16 - 2024/01/15 NanoSAURUS - Nanopartículas duplamente funcionalizadas para transportar mímicos de ácidos nucléicos contra a Staphylococcus aureus
EXPL/NAN-MAT/0209/2021
Researcher
Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2020/01/01 - 2023/12/31 Laboratory for Process Engineering, Environment, Biotechnology and Energy
UIDB/00511/2020
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2020/01/01 - 2023/12/31 Laboratory for Process Engineering, Environment, Biotechnology and Energy
UIDP/00511/2020
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2021/04/01 - 2023/06/30 2SMART - engineered Smart materials for Smart citizens
NORTE-01-0145-FEDER-000054
Researcher
Universidade do Porto Faculdade de Engenharia, Portugal
Comissao de Coordenacao e Desenvolvimento Regional do Norte
Concluded
2018/07/23 - 2021/07/22 uFISH - Integração de hibridação in situ fluorescente com microfluídica para deteção rápida de patogéneos alimentares
PTDC/EQU-EQU/31011/2017
Researcher
Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/08/01 - 2021/07/21 DelNAM - Strengthening the Research Area of Delivery of Nucleic acid mimics (NAMS) into bacteria to fight the antibiotic crisis
810685
Researcher
Universidade do Porto Faculdade de Engenharia, Portugal
EU Framework Programme for Research and Innovation Spreading Excellence and Widening Participation
Ongoing
2018/07/16 - 2021/07/15 ColorFISH - Development of a new colorimetric method for the rapid detection of Legionella spp. in water sources
PTDC/BTA-GES/29961/2017
Researcher
Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2018/07/01 - 2021/06/30 POLY-PREVENTT - Developing a multi-functionalized endotracheal tube to fight polymicrobial biofilms in a novel artificial ventilator-associated pneumonia model: a step closer to in vivo testing
POCI-01-0145-FEDER-029841
Researcher
Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2016/04/01 - 2019/09/30 LEPABE-2-ECO-INNOVATION
NORTE-01-0145-FEDER-000005
Researcher
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
Comissao de Coordenacao e Desenvolvimento Regional do Norte
Concluded
2016/07/01 - 2019/07/01 Coded-FISH - Development of a novel color-coded RING FISH method: an innovative tool for the detection of antibiotic resistant clinical pathogens
PTDC/DTP-PIC/4562/2014|16678
Researcher
Universidade do Minho, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2012/02 - 2015/01 ¿DNA mimics¿ - Development and application of DNA mimics for the rapid identification of pathogens
PIC/IC/82815/2007
Universidade do Minho, Portugal

Universidade do Porto Faculdade de Engenharia, Portugal

Universidade do Minho Centro de Engenharia Biológica, Portugal
Governo da República Portuguesa Ministério da Ciência Tecnologia e Ensino Superior

Fundação para a Ciência e a Tecnologia
Concluded
2010/04/01 - 2013/09/30 HELIWATER - Water and associated biofilms as a possible vector for Helicobacter pylori transmission among the human population
PTDC/BIA-MIC/108811/2008
Instituto de Biotecnologia e Bioengenharia, Portugal

Universidade do Minho, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2010/04/01 - 2013/03/31 HELIWATER - Water and associated biofilms as a possible vector for Helicobacter pylori transmission among the human population
Researcher
Universidade do Minho, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Pereira, Sara; Santos, Rita S.; Moreira, Luis; Guimarães, Nuno M.; Braeckmans, Kevin; De Smedt, Stefaan C.; Azevedo, Nuno F.. "Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes". In Fluorescence In-Situ Hybridization (FISH) for Microbial Cells, 87-96. Springer US, 2021.
  2. Guimarães, Nuno M.; Azevedo, Nuno F.; Almeida, Carina. "FISH Variants". In Fluorescence In-Situ Hybridization (FISH) for Microbial Cells, 17-33. Springer US, 2021.
    10.1007/978-1-0716-1115-9_2
  3. Pereira, S; Santos, RS; Moreira, L; Guimarães, NM; Braeckmans, K; De Smedt, SC; Azevedo, NF. "Delivery of Oligonucleotides into Bacteria by Fusogenic Liposomes". 2021.
    10.1007/978-1-0716-1115-9_6
  4. Moreira, L.; Guimarães, N.M.; Azevedo, N.F.. "Imaging strategies for bioinspired materials". 2016.
    10.1016/B978-0-08-100741-9.00008-5
  5. Santos-Silva, Filipe; Guimarães, Nuno. "Oncogenic Signaling in Gastric Carcinoma". In Gastric Carcinoma - Molecular Aspects and Current Advances. InTech, 2011.
    10.5772/21729
  6. Guimarães, Nuno; Fontenete, S.. "Other gram negative Biofilms". In Biofilm transcriptomics handbook: Quantifying gene expression from pathogenic bacterial biofilms. Braga, Portugal: Universidade do Minho - DEB, 2011.
Conference abstract
  1. Santos, João; Rita S. Santos; Guimarães, Nuno; Pinho, Eva; Azevedo, Nuno Filipe. "Delivery of Nucleic Acid Mimics (NAMs) into bacteria: study of the interaction between delivery vectors and the bacterial envelope and its importance for the development of novel therapeutics". Paper presented in Surfsafe Training School, Porto, 2022.
  2. Fontenete, S; Guimaraes, N; Leite, M; Madureira, P; Figueiredo, C; Wengel, J; Azevedo, NF. "FISH-BASED METHOD FOR IN VIVO DETECTION OF H. PYLORI IN GASTRIC MUCOSA USING ADVANCED LNA PROBES". 2013.
  3. Cerqueira, Laura; Azevedo, Nuno Filipe; Faria, Nuno Torres; Guimarães, Nuno; Figueiredo, Ceu; Keevil, Charles William; Vieira, Maria João. "New Diagnostic Method for the Detection of Clarithromycin Resistance in H. pylori Strains Using Peptide Nucleic Acid Probes". Paper presented in Helicobacter, 2009.
    Published
  4. Cerqueira, L; Azevedo, Nuno; Faria, N; Guimarães, Nuno; Figueiredo, C; Keevil, Cw; Vieira, MJ. "New diagnostic method for the detection of clarithromycin resistance in Helicobacter pylori strains using Peptide Nucleic Acid probes". Paper presented in XXII International Workshop on Helicobacter and related bacteria in chronic digestive inflammation and gastric cancer, Porto, 2009.
    Published
  5. Vilarinho, S.; Ferreira, RM; Guimarães, Nuno; Gomes, B.; Xiaogang, W; Oliveira, F; Freitas, D; et al. "Helicobacter pylori colonization of the adenotonsillar tissue: fact or fiction?". Paper presented in XXII International Workshop on Helicobacter and related bacteria in chronic digestive inflammation and gastric cancer, Porto, 2009.
    Published
  6. Cerqueira, L; Azevedo, Nuno; Guimarães, Nuno; Figueiredo, C; Keevil, Cw; Vieira, MJ. "Development of peptide nucleic acid probes for the detection of different point mutations conferring clarithromycin resistance in Helicobacter pylori 23S rRNA". Paper presented in XXIth International Workshop on Helicobacter and related bacteria in chronic digestive inflammation, Riga, 2008.
    Published
  7. Guimarães, Nuno; Azevedo, Nuno; Vieira, MJ; Figueiredo, C. "Water-exposed Helicobacter pylori presents decreased virulence properties". Paper presented in XXIth International Workshop on Helicobacter and related bacteria in chronic digestive inflammation, Riga, 2008.
    Published
  8. Guimarães, Nuno; Azevedo, Nuno; Figueiredo, C; Keevil, Cw; Vieira, MJ. "A Multiroute way of transmission for Helicobacter pylori". Paper presented in XX International Workshop on Helicobacter and related bacteria in chronic digestive inflammation, Istambul, 2007.
    Published
  9. Guimarães, Nuno; Azevedo, Nuno; Figueiredo, C; Keevil, C.W.; Vieira, MJ. "Development and Application of a Novel Peptide Nucleic Acid (PNA) Probe for the Specific Detection of Helicobacter pylori". Paper presented in XIX International Workshop on Helicobacter and related bacteria in chronic digestive inflammation, Wroclaw, 2006.
    Published
Conference poster
  1. Guimarães, Nuno. "Enhancing the delivery efficiency of Nucleic Acid Mimics into bacteria: Influence of liposome composition/functionalization". Paper presented in XV Spanish-Portuguese Conference on Controlled Drug Delivery, 2024.
  2. Monteiro, Carlos; Andrade, Stephanie; Maria João Ramalho; Rita S. Santos; Guimarães, Nuno; Azevedo, Nuno F.; Maria do Carmo Pereira; Joana Angélica Loureiro. "Folate-functionalized PLGA nanoparticles: A promising approach to target methicillin-resistant Staphylococcus aureus". Paper presented in 16th Encontro de Investigação Jovem (IJUP), 2023.
  3. Santos, João; Guimarães, Nuno; Pinho, Eva; Rita S. Santos; Azevedo, Nuno Filipe. "Delivery of Nucleic Acid Mimics into bacteria: effect of liposome composition in the delivery efficiency". Paper presented in CRS Young Scientists Meeting, 2023.
  4. Moreira, Luis; Pereira, Sara; Guimarães, Nuno; Santos, RS; Loureiro, J; Carmo Pereira, M; Azevedo, Nuno. "Effect of lipid concentration on the delivery efficiency of liposome-encapsulated nucleic acid mimics (LipoNAMs) on Escherichia coli". Paper presented in 29th ECCMID – European Congress of Clinical Microbiology and Infectious Diseases, 2019.
  5. Fontenete, S.; Carvalho, D; Guimarães, Nuno; Madureira, P; Figueiredo, C; Wengel, J; Azevedo, Nuno. "Evaluation of fluorescent Locked Nucleic Acid advanced probes by fluorescent in situ hybridization (FISH): a new analytical approach using FISHji". Paper presented in 3rd Symposium in Applied Bioimaging, 2014.
  6. Fontenete, S.; Guimarães, Nuno; Leite, M; Madureira, P; Figueiredo, C; Wengel, J; Azevedo, Nuno. "In Vivo Detection of Helicobacter Pylori in Gastric Mucosa Using Advanced LNA Probes By FISH". Paper presented in XXI International Roundtable of Nucleosides, Nucleotides and Nucleic Acids, 2014.
  7. Santos, Rita; Xiong R; Forier K; Dakwar GR; Remaut K; Guimarães, Nuno; Fontenete, S; et al. "Effect of gastric mucus on diffusion and in situ hybridization of LNA probes in Helicobacter pylori". Paper presented in European Symposium on Controlled Drug Delivery, 2014.
  8. Fontenete, S; Guimarães, Nuno; Leite, M; Figueiredo, C; Wengel, J; Azevedo, Nuno. "FISH-based method for in vivo detection of H. pylori in gastric mucosa using advanced LNA probes". Paper presented in XXVIth International Workshop on Helicobacter and related bacteria in chronic digestive inflammation and gastric cancer, 2013.
  9. Fontenete, S.; Guimarães, Nuno; Leite, M; Figueiredo, C; Wengel, J; Azevedo, Nuno. "Hybridization-based detection of Helicobacter pylori at human body temperature using advanced LNA probes". Paper presented in IV Symposium on Bioengineering, 2012.
  10. Fontenete, S; Guimarães, Nuno; Leite, M; Figueiredo, C; Wengel, J; Azevedo, Nuno. "Nucleic acid mimics hybridization at human body temperature in Helicobacter pylori". Paper presented in COST action TD1004 (Theragnostics Imaging and Therapy), 2012.
  11. Santos, RS; Guimarães, Nuno; Madureira, P; Azevedo, Nuno. "Impact of the external cellular milieu on the diffusion and hybridization of nucleic acids in bacterial cells". Paper presented in Cost action, 2012.
  12. Cerqueira, L; Azevedo, Nuno; Faria, N; Guimarães, Nuno; Figueiredo, C; Keevil, Cw; Vieira, MJ. "New diagnostic method for the detection of clarithromycin resistance in Helicobacter pylori strains using Peptide Nucleic Acid probes". Paper presented in XXII International Workshop on Helicobacter and related bacteria in chronic digestive inflammation and gastric cancer, 2009.
  13. Vilarinho, S; Ferreira, RM; Guimarães, Nuno; Gomes, B.; Xiaogang, W; Oliveira, F; Freitas, D; et al. "Helicobacter pylori colonization of the adenotonsillar tissue: fact or fiction?". Paper presented in XXII International Workshop on Helicobacter and related bacteria in chronic digestive inflammation and gastric cancer, 2009.
  14. Guimarães, Nuno; Azevedo, NF; Vieira, MJ; Figueiredo, C. "Water-exposed Helicobacter pylori presents decreased virulence properties". Paper presented in XXIst International Workshop on Helicobacter and related bacteria in chronic digestive inflammation and gastric cancer, 2008.
  15. Guimarães, Nuno; Azevedo, NF; Vieira, MJ; Figueiredo, C. "A multi-route way of transmission for Helicobacter pylori". Paper presented in XXth International Workshop on Helicobacter and related bacteria in chronic digestive inflammation and gastric cancer, 2007.
  16. Guimarães, Nuno; Coelho, C; Raposo, F; Monteiro, C; Laires, MJ; Bicho, M. "Polimorfismo-262 C/T da catalase e actividade da catalase numa população de mulheres pós-menopáusicas". Paper presented in VII Reunião da Sociedade Portuguesa de Genética Humana, 2003.
Journal article
  1. Moreira, Luís; Guimarães, Nuno Miguel; Santos, Rita Sobral; Loureiro, Joana Angélica; Pereira, Maria do Carmo; Azevedo, Nuno Filipe. "Oligonucleotide probes for imaging and diagnosis of bacterial infections". Critical Reviews in Biotechnology (2024): 1-20. http://dx.doi.org/10.1080/07388551.2024.2344574.
    Published • 10.1080/07388551.2024.2344574
  2. Moreira, Luís; Guimarães, Nuno M.; Santos, Rita S.; Loureiro, Joana A.; Pereira, Maria C.; Azevedo, Nuno F.. "Promising strategies employing nucleic acids as antimicrobial drugs". Molecular Therapy - Nucleic Acids (2024): 102122. http://dx.doi.org/10.1016/j.omtn.2024.102122.
    Published • 10.1016/j.omtn.2024.102122
  3. Stéphanie Andrade; Maria J. Ramalho; Sílvio B. Santos; Luís D. R. Melo; Rita S. Santos; Nuno Guimarães; Nuno F. Azevedo; Joana A. Loureiro; Maria C. Pereira. "Fighting Methicillin-Resistant Staphylococcus aureus with Targeted Nanoparticles". International Journal of Molecular Sciences (2023): https://doi.org/10.3390/ijms24109030.
    10.3390/ijms24109030
  4. Moreira, Luís; Guimarães, Nuno; Pereira, Sara; Santos, Rita S.; Loureiro, Joana A.; Ferreira, Rui M.; Figueiredo, Céu; Pereira, Maria C.; Azevedo, Nuno F.. "Engineered liposomes to deliver nucleic acid mimics in Escherichia coli". Journal of Controlled Release 355 (2023): 489-500. http://dx.doi.org/10.1016/j.jconrel.2023.02.012.
    Published • 10.1016/j.jconrel.2023.02.012
  5. Luís Moreira; Nuno M. Guimarães; Sara Pereira; Rita S. Santos; Joana A. Loureiro; Maria C. Pereira; Nuno F. Azevedo. "Liposome Delivery of Nucleic Acids in Bacteria: Toward In Vivo Labeling of Human Microbiota". ACS Infectious Diseases (2022): https://doi.org/10.1021/acsinfecdis.1c00601.
    10.1021/acsinfecdis.1c00601
  6. Pereira, Sara; Santos, Rita Sobral; Moreira, Luís; Guimarães, Nuno; Gomes, Mariana; Zhang, Heyang; Remaut, Katrien; et al. "Lipoplexes to Deliver Oligonucleotides in Gram-Positive and Gram-Negative Bacteria: Towards Treatment of Blood Infections". Pharmaceutics 13 7 (2021): 989. http://dx.doi.org/10.3390/pharmaceutics13070989.
    10.3390/pharmaceutics13070989
  7. Santos, R.S.; Lima, C.C.; Carvalho, D.; Meireles, F.; Guimarães, N.; Azevedo, N.F.. "Response surface methodology to optimize peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) in Saccharomyces cerevisiae". LWT - Food Science and Technology 80 (2017): 27-31. http://www.scopus.com/inward/record.url?eid=2-s2.0-85011841811&partnerID=MN8TOARS.
    10.1016/j.lwt.2017.01.077
  8. Fontenete S; Carvalho D; Guimarães N; Madureira P; Figueiredo C; Wengel J; Azevedo NF. "Application of locked nucleic acid-based probes in fluorescence in situ hybridization.". (2016): http://europepmc.org/abstract/med/26969040.
    10.1007/s00253-016-7429-4
  9. Fontenete, S.; Carvalho, D.; Lourenço, A.; Guimarães, N.; Madureira, P.; Figueiredo, C.; Azevedo, N.F.. "FISHji: New ImageJ macros for the quantification of fluorescence in epifluorescence images". Biochemical Engineering Journal 112 (2016): 61-69. http://www.scopus.com/inward/record.url?eid=2-s2.0-84963642833&partnerID=MN8TOARS.
    10.1016/j.bej.2016.04.001
  10. Fontenete, S.; Leite, M.; Guimarães, N.; Madureira, P.; Ferreira, R.M.; Figueiredo, C.; Wengel, J.; Azevedo, N.F.. "Towards fluorescence in vivo hybridization (FIVH) detection of H. pylori in gastric mucosa using advanced LNA probes". PLoS ONE 10 4 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84928573017&partnerID=MN8TOARS.
    10.1371/journal.pone.0125494
  11. Santos RS; Dakwar GR; Xiong R; Forier K; Remaut K; Stremersch S; Guimarães N; et al. "Effect of Native Gastric Mucus on in vivo Hybridization Therapies Directed at Helicobacter pylori.". (2015): http://europepmc.org/abstract/med/26645765.
    10.1038/mtna.2015.46
  12. Fontenete, Sílvia; Guimarães, Nuno; Wengel, Jesper; Azevedo, Nuno Filipe. "Prediction of melting temperatures in fluorescencein situhybridization (FISH) procedures using thermodynamic models". Critical Reviews in Biotechnology (2015): 1-12.
    10.3109/07388551.2014.993589
  13. Guimarães, N.M.; Azevedo, N.F.; Vieira, M.J.; Figueiredo, C.. "Water-induced modulation of Helicobacter pylori virulence properties". Memorias do Instituto Oswaldo Cruz 109 4 (2014): 414-419. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904318570&partnerID=MN8TOARS.
    10.1590/0074-0276140024
  14. Santos, R.S.; Guimarães, N.; Madureira, P.; Azevedo, N.F.. "Optimization of a peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method for the detection of bacteria and disclosure of a formamide effect". Journal of Biotechnology 187 (2014): 16-24. http://www.scopus.com/inward/record.url?eid=2-s2.0-84905256076&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2014.06.023
  15. Fontenete, S.; Guimarães, N.; Leite, M.; Figueiredo, C.; Wengel, J.; Azevedo, N.F.. "Hybridization-based detection of Helicobacter pylori at human body temperature using advanced Locked Nucleic Acid (LNA) probes". PLoS ONE 8 11 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84896723935&partnerID=MN8TOARS.
    10.1371/journal.pone.0081230
  16. Vilarinho, S.; Guimarães, N.M.; Ferreira, R.M.; Gomes, B.; Wen, X.; Vieira, M.J.; Carneiro, F.; Godinho, T.; Figueiredo, C.. "Helicobacter pylori colonization of the adenotonsillar tissue: Fact or fiction?". International Journal of Pediatric Otorhinolaryngology 74 7 (2010): 807-811. http://www.scopus.com/inward/record.url?eid=2-s2.0-79952032500&partnerID=MN8TOARS.
    10.1016/j.ijporl.2010.04.007
  17. Guimarães, N.; Azevedo, N.F.; Figueiredo, C.; Keevil, C.W.; Vieira, M.J.. "Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens". Journal of Clinical Microbiology 45 9 (2007): 3089-3094. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548678863&partnerID=MN8TOARS.
    10.1128/JCM.00858-07
  18. Azevedo, N.F.; Guimarães, N.; Figueiredo, C.; Keevil, C.W.; Vieira, M.J.. "A new model for the transmission of Helicobacter pylori: Role of environmental reservoirs as gene pools to increase strain diversity". Critical Reviews in Microbiology 33 3 (2007): 157-169. http://www.scopus.com/inward/record.url?eid=2-s2.0-34547157238&partnerID=MN8TOARS.
    10.1080/10408410701451922
  19. Guerra, A.; Rego, C.; Coelho, C.; Guimarães, N.; Thiran, C.; Aguiar, A.; Areias, J.C.; et al. "Polymorphism C242T of the gene of the p22phox subunit for nicotinamide adenine dinucleotide phosphate oxidase, and erythrocytic antioxidant enzymes, in patients with tetralogy of Fallot". Cardiology in the Young 17 3 (2007): 295-300. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249074748&partnerID=MN8TOARS.
    10.1017/S1047951107000534

Intellectual property

Patent
  1. Guimarães, Nuno; Wengel, Jesper; Rita S. Santos; Moreira, Luís; Pereira, Sara; Azevedo, Nuno Filipe. 2023. "Modified peptide-oligonucleotide conjugate".
  2. Guimarães, Nuno; Figueiredo, C; Azevedo, Nuno; Keevil, Cw; Vieira, MJ. 2009. "SONDA DE ÁCIDO PÉPTIDO NUCLEICO (PNA), ESTOJO E MÉTODO PARA DETECÇÃO ESPECÍFICA DE HELICOBACTER PYLORI E RESPECTIVAS APLICAÇÕES.". Portugal.
    Expired
  3. Guimarães, Nuno; Azevedo, Nuno; Figueiredo, C; Keevil, Cw; Vieira, MJ. 2008. "Nucleic Acid (PNA) Probe, Kit and Procedure for Specific Detection of Helicobacter pylori and Applications Thereof".
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2021/10/07 Nucleic acid mimics delivery into bacteria: fighting antibiotic resistance Innovation in Microbial Control e-conference
Online
2021/04/17 Nucleic acid mimics (NAMs): Applications in molecular therapy MOLECULAR THERAPIES AND TISSUE ENGINEERING
University of Coimbra (Online)
2019/05/08 Application of nucleic acid mimics for the detection and treatment of bacterial infections  DelNAM Summer School and workshop on “In vivo gene delivery”
(Ghent, Belgium)
2019/03/21 Design of oligonucleotides to target microorganisms Design and Synthesis of Nucleic Acid Mimics Workshop
(Odense, Denmark)
2016/11/03 New approaches on the application of nucleic acid mimics for bacteria detection and treatment LEPABE Meeting – Collaborative Research: New perspectives
LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, (Porto, Portugal)
2013 Fluorescence in vivo hybridization FISH | Fundamentals and Applications Workshop
Faculdade de Engenharia da Universidade do Porto (Porto, Portugal)
2012 Antibacterial DNA mimics as a novel technique for Helicobacter pylori treatment PhD program in Dental Medicine of the Faculty of Dental Medicine of University of Porto
Faculty of Dental Medicine of University of Porto (Porto, Portugal)
2008/09 Development of peptide nucleic acid probes for the detection of different point mutations conferring clarithromycin resistance in Helicobacter pylori 23S rRNA XXIth International Workshop on Helicobacter and related bacteria in chronic digestive inflammation
European Helicobacter Study Group (Riga, Latvia)
2008/03 Development of peptide nucleic acid probes for the detection of Helicobacter pylori clarithromycin resistance Encontro Luso-Francês de Helicobacter pylori
(Lisboa, Portugal)
2007/10 Development and Application of a Novel Peptide Nucleic Acid (PNA) Probe for the Specific Detection of Helicobacter pylori Semana da Engenharia da Universidade do Minho
Universidade do Minho (Braga, Portugal)
2007/10 Development and Application of a Novel Peptide Nucleic Acid (PNA) Probe for the Specific Detection of Helicobacter pylori XIX International Workshop on Helicobacter and related bacteria in chronic digestive inflammation
European Helicobacter Study Group (Wroclaw, Poland)
2003 Polimorfismo -262 C/T do catalase em mulheres pós-menopáusicas
Sociedade Portuguesa de Biologia (Lisboa, Portugal)

Event organisation

Event name
Type of event (Role)
Institution / Organization
2015/02/13 - Current LEPABE Day – From Science to innovation (2015/02/13 - 2015/02/13)
Meeting (Member of the Organising Committee)
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2021/09/30 - 2021/10/02 Ist Drug Delivery in Microbial Cells (DDMiC) Conference (2021/09/30 - 2021/10/02)
Conference (Member of the Organising Committee)
Universidade do Porto Faculdade de Engenharia, Portugal
2021/07/08 - 2021/07/09 1st Symposium on Oligonucleotide Technologies and Therapeutics@Portugal – OTP2021 (2021/07/08 - 2021/07/09)
Symposium
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2021/05/04 - 2021/05/06 2nd DelNAM Summer School (2021/05/04 - 2021/05/06)
Workshop (Member of the Organising Committee)
Universidade do Porto Faculdade de Engenharia, Portugal
2017/05/08 - 2017/05/09 PRINT-AID Recruitment Event (2017/05/08 - 2017/05/09)
Other (Member of the Organising Committee)
Universidade do Porto Faculdade de Engenharia, Portugal
2016/11/03 - 2016/11/03 LEPABE Meeting 2016 – Collaborative Research: New Approaches (2016/11/03 - 2016/11/03)
Meeting (Member of the Organising Committee)
Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia, Portugal
2013/04/18 - 2013/04/20 FISH – Fundamentals and Applications (2013/04/18 - 2013/04/20)
Workshop (President of the Organising Committee)
Universidade do Porto Faculdade de Engenharia, Portugal

Event participation

Activity description
Type of event
Event name
Institution / Organization
2023/03/17 - 2023/03/19 Symposium on Bioengineering, student-organised Bioengineering event. The main purpose is to present to academic community the most recent and innovative projects in the area of Bioengineering with applications on issues of our modern society.
Symposium
XIV Symposium on Bioengineering
Universidade do Porto Faculdade de Engenharia, Portugal
2013/07/18 - 2013/07/20 Workshop sobre os fundamentos e aplicação da técnica de fluorescence in situ hybridization (FISH)
Workshop
FISH – Fundamentals and Applications
Universidade do Porto Faculdade de Engenharia, Portugal

Jury of academic degree

Topic
Role
Candidate name (Type of degree)
Institution / Organization
2016/10 Development and validation of a PNA-FISH method for Vibrio detection
(Thesis) Main arguer
Francisa Marçal Queiroz Pinheiro Guedes (Master)
Universidade do Minho, Portugal
2013/10 Estudos de interferência para um produto baseado no método de PNA-FISH
(Thesis) Main arguer
Teresa Maria Conde Lopes (Master)
Universidade do Minho, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2016/09/01 - 2023/02/01 Microbiologia geral Mestrado Integrado em Bioengenharia (Mestrado integrado) Universidade do Porto Faculdade de Engenharia, Portugal
Distinctions

Other distinction

2008 Young Scientist Award
2007 Young Scientist Award
2006 Young Scientist Award