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Frederico Aires da Silva is an Assistant Professor and Principal Investigator at Faculty of Veterinary Medicine, University of Lisbon, Portugal. He is the leader of the Antibody Engineering (AE) group at CIISA´s Microbiology and Immunology Laboratory. This dynamic AE team employs state-of-the-art technologies in comparative medicine to bridge the gap between Human and Animal diseases in a One Health context, aiming to revolutionize treatment options for shared health challenges. The AE group has achieved significant milestones, including the development of antibodies against canine lymphoma, breast cancer, SARS-CoV-2 and antibodies to overcome the blood-brain barrier for treating CNS disorders. He has authored >42 publications in peer-reviewed journals, 3 book chapters, and 8 patents, and H-index = 20. He was the co-founder of TechnoAntibodies (TechnoPhage SA) and Vector B2B. For more details visit: Lab homepage: http://faslab.fmv.ulisboa.pt/ Ciência Vitae ID: 4410-6DA0-C74C ORCID: https://orcid.org/0000-0001-7858-4067 Scopus Author ID: 56684960100
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Identification

Personal identification

Full name
Frederico Nuno Castanheira Aires da Silva

Citation names

  • Frederico Aires da Silva

Author identifiers

Ciência ID
4410-6DA0-C74C
ORCID iD
0000-0002-3821-419X

Addresses

  • Faculdade de Medicina Veterinária - Universidade de Lisboa. Av. Universidade Técnica., 1300-477, Lisboa,Lisboa, Portugal, Lisboa, Portugal (Professional)

Websites

Knowledge fields

  • Medical and Health Sciences - Health Sciences

Languages

Language Speaking Reading Writing Listening Peer-review
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Education
Degree Classification
2004 - 2008
Concluded
 Pharmaceutical Sciences (Doutoramento)
Major in Microbiology
Universidade de Lisboa, Portugal
"Recombinant Intracellular Antibodies for Molecular Gene Therapy of Hiv-1 Infection" (THESIS/DISSERTATION)
2002 - 2004
Concluded
 Advanced Biomolecular Methods (Mestrado)
Universidade de Aveiro, Portugal
"Camelized’ Rabbit VH Single-Domain Intrabodies Efficiently Binds Vif Protein and " (THESIS/DISSERTATION)
2001
Concluded
 Biotechnology Engineering (Licenciatura)
Universidade do Algarve, Portugal
"Selection of recombinant antibodies by synthetic phage display antibodies libraries" (THESIS/DISSERTATION)
Affiliation

Science

Category
Host institution 		Employer
2013 - Current Principal Investigator (Research) Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2019/10/01 - 2019/12/31 Visiting Researcher (Research) National Cancer Institute Center for Cancer Research, United States
2012/11 - 2012/12 Visiting Researcher (Research) Shanghai Institute of Materia Medica Chinese Academy of Sciences Center of Pharmaceutics Research, China

Teaching in Higher Education

Category
Host institution 		Employer
2020 - Current Assistant Professor (University Teacher) Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal

Others

Category
Host institution 		Employer
2019 - Current Co-founder and executive board member of the Collaborative Laboratory (Co-Lab) Vector B2B Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2014 - Current Scientific Adviser TechnoPhage SA, Portugal
2008 - 2013 Co-founder and Scientific Unit Manger - TechnoAntibodies TechnoPhage SA, Portugal
Projects

Grant

Designation Funders
2019 - 2026 Vector B2B - Drug Developing – Association for Research in Biotechnology
Vector B2B
Principal investigator
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
 Fundação para a Ciência e a Tecnologia
Ongoing
2021/01/01 - 2025/12/31 Associate Laboratory for Animal and Veterinary Sciences
LA/P/0059/2020
10.54499/LA/P/0059/2020
Researcher
Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal

Universidade do Porto Centro de Estudos de Ciencia Animal, Portugal

Universidade de Trás-os-Montes e Alto Douro Centro de Ciência Animal e Veterinária, Portugal

Universidade de Trás-os-Montes e Alto Douro, Portugal

Universidade do Porto Instituto de Ciências Tecnologias e Agroambiente, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2022/01/17 - 2025/01/16 SmartBox: Engineering the first generation of ROS responsive ADCs
PTDC/QUI-OUT/3989/2021
Researcher
Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal

Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2022/01/02 - 2025/01/01 Development of potent and broadly neutralizing antibodies fragments for COVID-19 derived from domestic cats naturally infected with SARS-CoV-2
PTDC/CVT-CVT/0149/2021
Principal investigator
Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal

Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento, Portugal

Universidade de Lisboa Instituto de Medicina Molecular João Lobo Antunes, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2023/03/12 - 2024/09/11 Desenvolvimento de novos conjugados anticorpo-enzima para imunoterapia do cancro através de edição do glicocálice no carcinoma mamário felino
2022.07903.PTDC
10.54499/2022.07903.PTDC
Researcher
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal

Universidade Nova de Lisboa Associação para a Inovação e Desenvolvimento da FCT, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2023/01/01 - 2024/06/30 ArmCART: Promoting T-cell memory and innate immunity to increase the efficacy of CAR-T cells
2022.07746.PTDC
10.54499/2022.07746.PTDC
Researcher
Fundação para a Ciência e a Tecnologia
Ongoing
2022/05/17 - 2024/06/30 TRIFECTA - Development of a novel trispecific antibody for the anticancer immune targeting of non-Hodgkin Lymphoma
EXPL/CVT-CVT/1285/2021
10.54499/EXPL/CVT-CVT/1285/2021
Researcher
Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal

Universidade de Lisboa Faculdade de Farmácia, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal

Instituto Politécnico de Coimbra Centro de Estudos de Recursos Naturais Ambiente e Sociedade, Portugal

Universidade de Lisboa Instituto de Investigação do Medicamento, Portugal
Fundação para a Ciência e a Tecnologia
Ongoing
2022 - 2024 Development of Recombinant Antibodies with High Potency and Neutralization for Treatment and Prevention of SARS-CoV-2 infection.
PGG/013823/2021
Principal investigator
Gilead GÉNESE
Ongoing
2022/01/03 - 2023/07/02 A novel tolerogenic liposome-based allergen immunotherapy for dogs in the horizon
EXPL/CVT-CVT/1458/2021
Researcher
Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2020/01/01 - 2022/12/31 LYMPHOTARGET - Development of imunoliposomes as a drug delivery system for the treatment of non-Hodgkin Lymphoma
PGG/050/2019 - Projeto de Investigação
 Gilead Sciences Europe Ltd
Ongoing
2020 - 2022 Development of immunoliposomes for specific targeting of non-Hodgkin’s lymphoma treatment
PGG/050/2019
Researcher
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
 Gilead GÉNESE
Concluded
2021/01/01 - 2021/12/30 Staphylococcus targeting liposomes: a novel drug delivery strategy for MRSA infections
INOV20Set – 04
Researcher
Universidade de Lisboa Faculdade de Farmácia, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2018 - 2021 Development of a novel class of antibody-drug conjugates molecules for cancer treatment
PTDC/BTM-SAL/32085/2017
Principal investigator
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2019/01/01 - 2019/12/31 Center for Interdisciplinary Research in Animal Health
UID/CVT/00276/2019
Researcher
Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal

Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2018/04/01 - 2019/03/31 In vitro efficacy of a brain targeted antibiotic encapsulated liposome for treatment of bacterial meningitis.
UID/CVT/00276/2014
Researcher
Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal, Portugal
 Universidade de Lisboa Centro de Investigação Interdisciplinar em Sanidade Animal

Fundação para a Ciência e a Tecnologia
Concluded
2017 - 2019 Development of anti-gp120/gp41 bispecific antibodies for HIV infection treatment
PGG/015/2016
Principal investigator
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
 Gilead GÉNESE
Concluded
2016 - 2019 Overcoming the Brain Drug Delivery Bottleneck: Development of single domain antibodies for brain targeting and drug delivery across the Blood-Brain Barrier
PTDC/BBB-BIO/0508/2014
Principal investigator
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2016 - 2019 Peptides for blood-brain barrier transmigration and drug delivery - novel therapies for the central nervous system
PTDC/BBB-NAN/1578/2014
Researcher
Instituto de Medicina Molecular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2016 - 2019 Alzheimer3D: Trifunctional Conjugates for the Selective Drug Delivery in Alzheimer’s Disease
PTDC/QEQ-MED/5512/2014
Researcher
Universidade de Lisboa Faculdade de Farmácia, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2014/01 - 2018/12 Starting Grant under the FCT Investigator Program
IF/01010/2013/CP1183/CT0001
 Fundação para a Ciencia e Tecnologia - FCT
2013 - 2016 TA101 GoClin
606532
606532
Principal investigator
TechnoPhage SA, Portugal
 European Commission Seventh Framework Programme for Research and Technological Development
Concluded
2012 - 2015 Development of single domains antibodies for therapeutic applications in inflammatory diseases
QREN InnovAB
Principal investigator
Quadro de Referência Estratégico Nacional (QREN)
2011/03/01 - 2014/08/31 Albumin binding-domain fusions to improve protein pharmacokinetics
PTDC/SAU-FAR/115846/2009
Co-Principal Investigator (Co-PI)
Universidade de Lisboa Instituto Superior Técnico, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2009 - 2012 Translational research in Alzheimer´s disease
QREN Project - PPS 3
Principal investigator
TechnoPhage SA, Portugal
Concluded
2008/01/01 - 2011/04/30 A Novel Lentiviral Target-Specific Strategy for Molecular and Gene Therapy
PTDC/BIA-BCM/64275/2006
Researcher
Fundação para a Ciência e a Tecnologia
Concluded
2004/10/01 - 2008/09/30 Recombinant Intracellular Antibodies For Molecular Gene Therapy of HIV-1 Infection
SFRH/BD/17039/2004
PhD Student Fellow
Fundação para a Ciência e a Tecnologia
Concluded

Contract

Designation Funders
2014 - 2018 FCT Investigator Starting Grant Program
F/01010/2013
Principal investigator
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
Outputs

Publications

Book chapter
  1. Frederico Aires da Silva. "Novel Diagnostic and Therapeutic Immunologic Strategies to Overcome Infectious, Oncologic and Neurodegenerative isorders". In Advances in Animal Health, Medicine and Production, 1-575. Springer Nature Switzerland AG 2020: Springer Nature, 2020.
    Published
  2. Silva, Frederico; Côrte-Real, S.; Aires Da Silva, F.; Gonçalves, J.; Sofia Côrte-Real; Frederico Aires da Silva; João Gonçalves. "Development of Antibody Fragments for Therapeutic Applications.". 2011.
Conference abstract
  1. Pinto, Marta; Marto, Joana; Solange Gil; Bizarro, Ana; Fernandes, Beatriz; Rodolfo Oliveira Leal; Lídia Gonalves; et al. "A novel tolerogenic liposome-based allergen immunotherapy for dogs in the horizon". Paper presented in 1st Meeting of the Associate Laboratiry for Animal and Veterinary Sciences – AL4AnimalS, Lisboa, 2022.
Conference poster
  1. Rafaela Marimon; Moutinho, Isa; Ana André; Ana Leonardo; Luís Tavares; Frederico Aires da Silva. "An immunotoxin derived from pseudomonas aeruginosa as a promising approach for the treatment of Triple Negative Breast Cancer". Paper presented in 3rd Meeting of the Associate Laboratory for Animal and Veterinary Sciences (Al4animals) - Comparative and Translational Medicine and Biotechnology, 2023.
  2. Moutinho, Isa; Mafalda Henriques; Sara Cardoso; Teresa Penha Coutinho; Luís Tavares; Solange Gil; Telmo Nunes; Frederico Aires da Silva. "Antibodies derived from cats naturally infected with SARS CoV 2 as a promising approach for COVID 19 treatment and prevention". Paper presented in 3rd Meeting of the Associate Laboratory for Animal and Veterinary Sciences (Al4animals) - Comparative and Translational Medicine and Biotechnology,, 2023.
  3. Henriques, Mafalda; Moutinho, Isa; Sara Cardoso; Luís Tavares; Solange Gil; Telmo Nunes; Frederico Aires da Silva. "SARS-CoV-2 em animais domésticos: avaliação serológica de cães e gatos provenientes de agregados familiares com casos confirmados de COVID-19". Paper presented in Congresso de Saúde Pública, 2023.
  4. Moutinho, Isa; Mafalda Henriques; Sara Cardoso; Teresa Coutinho; Luís Tavares; Solange Gil; Telmo Nunes; Frederico Aires da Silva. "Cat-derived antibody fragments as a promising approach for developing a new generation of potent and broadly neutralizing antibodies for SARS-CoV-2 treatment and prevention". Paper presented in Antibody Engineering & Therapeutics Europe, 2023.
  5. Moutinho, Isa; Sara Cardoso; Mafalda Henriques; Teresa Coutinho; Luís Tavares; Telmo Nunes; Solange Gil; Frederico Aires da Silva. "Susceptibility of pets to SARS-CoV-2 Infection: Serological survey for SARS-CoV-2 antibodies in cats and dogs from households diagnosed with COVID-19". Paper presented in 2º Encontro do Laboratório Associado para a Ciência Animal e Veterinária (AL4AnimalS), 2023.
  6. Henriques, Mafalda; Moutinho, Isa; Sara Cardoso; Teresa Coutinho; Sandra I. Aguiar; Sara Nogueira; Luís Tavares; et al. "Susceptibility of pets to SARS-CoV-2 Infection: Serological survey for SARS-CoV-2 antibodies in a cat population of Lisbon, Portugal". Paper presented in 2º Encontro do Laboratório Associado para a Ciência Animal e Veterinária (AL4AnimalS), 2023.
  7. Henriques, Mafalda; Moutinho, Isa; Sara Cardoso; Solange Gil; Telmo Nunes; Frederico Aires da Silva. "Caracterização da imunidade ao SARS-CoV-2 em cães e gatos provenientes de agregados familiares com casos confirmados de COVID-19". Paper presented in Pandemias na Era da Globalização, 2023.
  8. Moutinho, Isa; Sara Cardoso; Teresa Coutinho; Aguiar, Sandra I; Sara Nogueira; Penha-Gonçalves, Carlos; Jocelyne Demengeot; et al. "Develpment of potent and broadly neutralizing antibodies fragments for COVID-19 derived from domestic cats naturally infected with SARS-CoV-2". Paper presented in CIISA Congress 2022- Innovation in Animal, Veterinary and Biomedical Research, 2022.
  9. Grilo, Andreia; Barroso, Maria Cristina; Aguiar, Sandra I; Frederico Aires da Silva; São Braz, Berta. "MDR1 1-delta mutation detection in Barbado da Terceira in Portugal.". Paper presented in CIISA CONGRESS 2022 - Innovation in Animal, Veterinary and Biomedical Research, 2022.
  10. Moutinho, Isa; Henriques, Mafalda; Sara Cardoso; Teresa Coutinho; Sandra Aguiar ; Sara Nogueira; João Gonçalves; et al. "Development of potent and broadly neutralizing antibodies fragments for COVID-19 derived from domestic cats naturally infected with SARS-CoV-2". Paper presented in Rede Saúde - Universidade de Lisboa, 2022.
  11. Moutinho, Isa; Sara Cardoso; Henriques, Mafalda; Teresa Coutinho; Sara Nogueira; Sandra Aguiar; Luís Tavares; et al. "Susceptibility of pets to SARS-CoV-2 Infection: Serological survey for SARS-CoV-2 antibodies in cats and dogs from households with confirmed cases of COVID-19". Paper presented in Innovation in Animal, Veterinary and Biomedical Research, 2022.
  12. Moutinho, Isa; Henriques, Mafalda; João Gonçalves; Telmo Nunes; Solange Gil; Luís Tavares; Frederico Aires da Silva. "Development of potent and broadly neutralizing single domain antibodies for SARS CoV 2 treatment and prevention". Paper presented in Innovation in Animal, Veterinary and Biomedical Research, 2022.
  13. Brito, Paula; Rita Acúrcio; Inês Raquel Iria; Ana Catarina Cunha Santos; Ana Barbas; Frederico Aires da Silva; Professor João Manuel Bras Goncalves; Miguel Cardoso. "P545 Antigenic response to CT-P13 and remicade in inflammatory bowel disease patients shows similar epitope recognition". Paper presented in 13th Congress of the European Crohn’s and Colitis Organization, Vienna, Austria, 2018.
Journal article
  1. André, Ana S.; Dias, Joana N. R.; Aguiar, Sandra I.; Leonardo, Ana; Nogueira, Sara; Amaral, Joana D.; Fernandes, Célia; et al. "Panobinostat-loaded folate targeted liposomes as a promising drug delivery system for treatment of canine B-cell lymphoma". Frontiers in Veterinary Science 10 (2023): http://dx.doi.org/10.3389/fvets.2023.1236136.
    10.3389/fvets.2023.1236136
  2. André, Ana S.; Dias, Joana N. R.; Aguiar, Sandra; Nogueira, Sara; Bule, Pedro; Carvalho, Joana Inês; António, João P. M.; et al. "Rabbit derived VL single-domains as promising scaffolds to generate antibody–drug conjugates". Scientific Reports 13 1 (2023): http://dx.doi.org/10.1038/s41598-023-31568-x.
    10.1038/s41598-023-31568-x
  3. Dias, Joana N. R.; Almeida, André; S. André, Ana; Aguiar, Sandra I; Bule, Pedro; Nogueira, Sara; Oliveira, Soraia S; et al. "Characterization of the canine CD20 as a therapeutic target for comparative passive immunotherapy". Scientific Reports 12 1 (2022): http://dx.doi.org/10.1038/s41598-022-06549-1.
    10.1038/s41598-022-06549-1
  4. Istrate, Claudia; Marques, Jéssica; Bule, Pedro; Correia, Sílvia; Aires-da-Silva, Frederico; Duarte, Marlene; Reis, Ana Luísa; et al. "In Silico Characterization of African Swine Fever Virus Nucleoprotein p10 Interaction with DNA". Viruses 14 11 (2022): 2348. http://dx.doi.org/10.3390/v14112348.
    10.3390/v14112348
  5. Barroso, Maria Cristina; Grilo, Andreia; Aguiar, Sandra; Aires da Silva, Frederico; São Braz, Berta. "Occurrence of MDR1 1-delta mutation in herding dog breeds in Portugal". Frontiers in Veterinary Science 9 (2022): http://dx.doi.org/10.3389/fvets.2022.990884.
    10.3389/fvets.2022.990884
  6. André, Ana S.; Moutinho, Isa; Dias, Joana N. R.; Aires-da-Silva, Frederico. "In vivo Phage Display: A promising selection strategy for the improvement of antibody targeting and drug delivery properties". Frontiers in Microbiology 13 (2022): http://dx.doi.org/10.3389/fmicb.2022.962124.
    10.3389/fmicb.2022.962124
  7. "Diazaborines Are a Versatile Platform to Develop ROS-Responsive Antibody Drug Conjugates**". Angewandte Chemie International Edition (2021): http://dx.doi.org/10.1002/anie.202109835.
    10.1002/anie.202109835
  8. Sandra Isabel Aguiar; Joana N. R. Dias; Ana André; Marta Lisete Silva; Diana Martins; Belmira Carrapiço; Miguel Castanho; et al. "Highly Specific Blood-Brain Barrier Transmigrating Single-Domain Antibodies Selected by an In Vivo Phage Display Screening". Pharmaceutics (2021): https://www.mdpi.com/1999-4923/13/10/1598.
    10.3390/pharmaceutics13101598
  9. Pedro Bule; Sandra Isabel Aguiar; Frederico Aires da Silva; Joana Nunes Ribeiro Dias. "Chemokine-Directed Tumor Microenvironment Modulation in Cancer Immunotherapy". International Journal of Molecular Sciences (2021): https://www.mdpi.com/1422-0067/22/18/9804.
    10.3390/ijms22189804
  10. Magda Ferreira; Maria Ogren; Joana N. R. Dias; Marta Silva; Solange Gil; Luís Tavares; Frederico Aires da Silva; Gaspar MM; Sandra Isabel Aguiar. "Liposomes as Antibiotic Delivery Systems: A Promising Nanotechnological Strategy against Antimicrobial Resistance". Molecules (2021): https://www.mdpi.com/1420-3049/26/7/2047.
    10.3390/molecules26072047
  11. Singh, Sumnima; Thompson, Jessica A.; Yilmaz, Bahtiyar; Li, Hai; Weis, Sebastian; Sobral, Daniel; Truglio, Mauro; et al. "Loss of a-gal during primate evolution enhanced antibody-effector function and resistance to bacterial sepsis". Cell Host & Microbe (2021): http://dx.doi.org/10.1016/j.chom.2020.12.017.
    10.1016/j.chom.2020.12.017
  12. Ferreira, M.C.; Pinto, Sandra N.; Aires da Silva, Frederico; Bettencourt, Ana; Aguiar, Sandra I; Gaspar, Maria Manuela. "Liposomes as a nanoplatform to improve the delivery of antibiotics into Staphylococcus aureus biofilms". (2021): http://hdl.handle.net/10400.5/23329.
    10.3390/pharmaceutics13030321
  13. Bule, Pedro; Aguiar, Sandra Isabel; Aires Da Silva, Frederico; Dias, Joana Nunes Ribeiro. "Chemokine-directed tumor microenvironment modulation in cancer immunotherapy". (2021): http://hdl.handle.net/10400.5/22459.
    10.3390/ijms22189804
  14. Ferreira, M.C.; Pinto, Sandra N.; Aires da Silva, Frederico; Bettencourt, Ana; Aguiar, Sandra I; Gaspar, Maria Manuela. "Liposomes as a nanoplatform to improve the delivery of antibiotics into Staphylococcus aureus biofilms". (2021): http://hdl.handle.net/10400.5/23329.
    10.3390/pharmaceutics13030321
  15. Aguiar, Sandra Isabel; Dias, Joana N. R.; S. André, Ana; Silva, Lisete M.; Martins, Diana; Carrapiço, Belmira; Castanho, Miguel; et al. "Highly specific blood-brain barrier transmigrating single-domain antibodies selected by an In Vivo phage display screening". (2021): http://hdl.handle.net/10400.5/22940.
    10.3390/pharmaceutics13101598
  16. Alves, F.; Prata, S.; Nunes, T.; Gomes, J.; Aguiar, S.; Aires da Silva, F.; Tavares, L.; Almeida, V.; Gil, S.. "Canine parvovirus: a predicting canine model for sepsis". BMC Veterinary Research 16 1 (2020): http://dx.doi.org/10.1186/s12917-020-02417-0.
    10.1186/s12917-020-02417-0
  17. Calado, Rita; Duarte, Joana; Borrego, Pedro; Marcelino, José Maria; Bártolo, Inês; Martin, Francisco; Figueiredo, Inês; et al. "A Prime-Boost Immunization Strategy with Vaccinia Virus Expressing Novel gp120 Envelope Glycoprotein from a CRF02_AG Isolate Elicits Cross-Clade Tier 2 HIV-1 Neutralizing Antibodies". Vaccines 8 2 (2020): 171. http://dx.doi.org/10.3390/vaccines8020171.
    10.3390/vaccines8020171
  18. Ministro, Joana H.; Oliveira, Soraia S.; Oliveira, Joana G.; Cardoso, Miguel; Aires-da-Silva, Frederico; Corte-Real, Sofia; Goncalves, Joao. "Synthetic antibody discovery against native antigens by CRISPR/Cas9-library generation and endoplasmic reticulum screening". Applied Microbiology and Biotechnology 104 6 (2020): 2501-2512. http://dx.doi.org/10.1007/s00253-020-10423-3.
    10.1007/s00253-020-10423-3
  19. Alves, F.; Prata, S.; Nunes, Telmo; Gomes, J.; Aguiar, Sandra; Aires da Silva, Frederico; Tavares, Luis; Almeida, Virgilio; Gil, Solange. "Canine parvovirus : a predicting canine model for sepsis". (2020): http://hdl.handle.net/10400.5/24068.
    10.1186/s12917-020-02417-0
  20. Calado, Rita; Duarte, Joana; Borrego, Pedro; Marcelino, José Maria; Bártolo, Inês; Martin, Francisco; Figueiredo, Inês; et al. "A Prime-Boost Immunization Strategy with Vaccinia Virus Expressing Novel gp120 Envelope Glycoprotein from a CRF02_AG Isolate Elicits Cross-Clade Tier 2 HIV-1 Neutralizing Antibodies". (2020): http://hdl.handle.net/10451/58874.
    10.3390/vaccines8020171
  21. Dias, Joana N.R.; Lopes, Mariana; Peleteiro, Conceição; Vicente, Gonçalo; Nunes, Telmo; Mateus, Luísa; Aires-da-Silva, Frederico; Tavares, Luís; Gil, Solange. "Canine multicentric lymphoma exhibits systemic and intratumoral cytokine dysregulation". Veterinary Immunology and Immunopathology 218 (2019): 109940. http://dx.doi.org/10.1016/j.vetimm.2019.109940.
    10.1016/j.vetimm.2019.109940
  22. J. Goncalves; M. Santos; R. Acurcio; I. Iria; L. Gouveia; P. Matos Brito; A. Catarina Cunha-Santos; et al. "Antigenic response to CT-P13 and infliximab originator in inflammatory bowel disease patients shows similar epitope recognition". Alimentary Pharmacology & Therapeutics (2018): https://doi.org/10.1111%2Fapt.14808.
    10.1111/apt.14808
  23. Joana N.R. Dias; Sandra I. Aguiar; Diane M. Pereira; Ana S. André; Lurdes Gano; João D.G. Correia; Belmira Carrapiço; et al. "The histone deacetylase inhibitor panobinostat is a potent antitumor agent in canine diffuse large B-cell lymphoma". Oncotarget 9 47 (2018): https://doi.org/10.18632%2Foncotarget.25580.
    10.18632/oncotarget.25580
  24. Dias, J.N.R.; André, A.S.; Aguiar, S.I.; Ministro, J.; Oliveira, J.; Peleteiro, M.C.; Rütgen, B.; et al. "Establishment of a bioluminescent canine B-cell lymphoma xenograft model for monitoring tumor progression and treatment response in preclinical studies". PLoS ONE 13 12 (2018): http://www.scopus.com/inward/record.url?eid=2-s2.0-85059225750&partnerID=MN8TOARS.
    10.1371/journal.pone.0208147
  25. Cantante, C.; Lourenço, S.; Morais, M.; Leandro, J.; Gano, L.; Silva, N.; Leandro, P.; et al. "Albumin-binding domain from Streptococcus zooepidemicus protein Zag as a novel strategy to improve the half-life of therapeutic proteins". Journal of Biotechnology 253 (2017): 23-33. http://www.scopus.com/inward/record.url?eid=2-s2.0-85019767367&partnerID=MN8TOARS.
    10.1016/j.jbiotec.2017.05.017
  26. Gouveia, Z.; Carlos, A.R.; Yuan, X.; Aires-da-Silva, F.; Stocker, R.; Maghzal, G.J.; Leal, S.S.; et al. "Characterization of plasma labile heme in hemolytic conditions". FEBS Journal 284 19 (2017): 3278-3301. http://www.scopus.com/inward/record.url?eid=2-s2.0-85029000547&partnerID=MN8TOARS.
    10.1111/febs.14192
  27. Neves, Vera; Silva, Frederico Aires da; Morais, Maurício; Gano, Lurdes; Ribeiro, Elisabete; Pinto, Antónia; Aguiar, Sandra; et al. "Novel peptides derived from dengue virus capsid protein translocate reversibly the blood-brain barrier through a receptor-free mechanism". (2017): http://hdl.handle.net/10451/32811.
    10.1021/acschembio.7b00087
  28. Vera Neves; Frederico Aires-da-Silva; Sofia Corte-Real; Miguel A.R.B. Castanho. "Antibody Approaches To Treat Brain Diseases". Trends in Biotechnology 34 1 (2016): 36-48. https://doi.org/10.1016%2Fj.tibtech.2015.10.005.
    10.1016/j.tibtech.2015.10.005
  29. Cunha-Santos, Catarina; Figueira, Tiago N.; Borrego, Pedro; Oliveira, Soraia S.; Rocha, Cheila; Couto, Andreia; Cantante, Cátia; et al. "Development of synthetic light-chain antibodies as novel and potent HIV fusion inhibitors". AIDS 30 11 (2016): 1691-1701. http://hdl.handle.net/10400.26/15096.
    10.1097/QAD.0000000000001108
  30. Morais M; Cantante C; Gano L; Santos I; Lourenço S; Santos C; Fontes C; et al. "Biodistribution of a (67)Ga-labeled anti-TNF VHH single-domain antibody containing a bacterial albumin-binding domain (Zag).". (2014): http://europepmc.org/abstract/med/24530366.
    10.1016/j.nucmedbio.2014.01.009
  31. da Silva FA; Li M; Rato S; Maia S; Malhó R; Warren K; Harrich D; et al. "Recombinant rabbit single-chain antibodies bind to the catalytic and C-terminal domains of HIV-1 integrase protein and strongly inhibit HIV-1 replication.". (2012): http://europepmc.org/abstract/med/23586912.
    10.1002/bab.1034
  32. Oliveira SS; Aires da Silva F; Lourenco S; Freitas-Vieira A; Cunha Santos AC; Goncalves J. "Assessing combinatorial strategies to multimerize libraries of single-domain antibodies.". (2012): http://europepmc.org/abstract/med/23586829.
    10.1002/bab.1011
  33. Aires da Silva F; Corte-Real S; Goncalves J; da Silva, Frederico Aires; Corte-Real, Sofia; Goncalves, Joao. "Recombinant antibodies as therapeutic agents: pathways for modeling new biodrugs.". (2008): http://europepmc.org/abstract/med/18778112.
    10.2165/00063030-200822050-00003
  34. Encarnação JM; Rosa L; Rodrigues R; Pedro L; da Silva FA; Gonçalves J; Ferreira GN. "Piezoelectric biosensors for biorecognition analysis: application to the kinetic study of HIV-1 Vif protein binding to recombinant antibodies.". (2007): http://europepmc.org/abstract/med/17566584.
    10.1016/j.jbiotec.2007.04.010
  35. Santa-Marta M; Aires da Silva F; Fonseca AM; Rato S; Goncalves J; Santa-Marta, Mariana; da Silva, Frederico Aires; et al. "HIV-1 Vif protein blocks the cytidine deaminase activity of B-cell specific AID in E. coli by a similar mechanism of action.". (2007): http://europepmc.org/abstract/med/16580072.
    10.1016/j.molimm.2006.02.005
  36. Encarnacao, Joao M.; Rosa, Luis; Rodrigues, Rogerio; Pedro, Luisa; da Silva, Frederico Aires; Goncalves, Joao; Ferreira, Guilherme N. M.. "Piezoelectric biosensors for biorecognition analysis". (2007): http://hdl.handle.net/10451/21262.
    http://dx.doi.org/10.1016/j.jbiotec.2007.04.010
  37. Ferreira, Guilherme N. M.; Encarnacao, Joao M.; Rosa, Luis; Rodrigues, Rogrio; Breyner, Roberta; Barrento, Sara; Pedro, Luisa; et al. "Recombinant single-chain variable fragment and single domain antibody piezoimmunosensors for detection of HIV1 virion infectivity factor". (2007): http://hdl.handle.net/10451/21264.
    http://dx.doi.org/10.1016/j.bios.2007.04.022
  38. Corte-Real S; Collins C; Aires da Silva F; Simas JP; Barbas CF; Chang Y; Moore P; Goncalves J. "Intrabodies targeting the Kaposi sarcoma-associated herpesvirus latency antigen inhibit viral persistence in lymphoma cells.". (2005): http://europepmc.org/abstract/med/16091453.
    10.1182/blood-2005-04-1627
  39. Santa-Marta M; da Silva FA; Fonseca AM; Goncalves J. "HIV-1 Vif can directly inhibit apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G-mediated cytidine deamination by using a single amino acid interaction and without protein degradation.". (2005): http://europepmc.org/abstract/med/15611076.
    10.1074/jbc.m409309200
  40. Aires da Silva F; Costa MJ; Corte-Real S; Goncalves J. "Cell type-specific targeting with sindbis pseudotyped lentiviral vectors displaying anti-CCR5 single-chain antibodies.". (2005): http://europepmc.org/abstract/med/15761262.
    10.1089/hum.2005.16.223
  41. Silva, Frederico. "Camelized rabbit-derived VH single-domain intrabodies against Vif strongly neutralize HIV-1 infectivity.". J Mol Biol. (2004):
    10.1016/j.jmb.2004.04.062
  42. Silva, Frederico. "Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication.". J Biol Chem. (2002): http://www.jbc.org/content/277/35/32036.long.
Journal issue
  1. Oliveira, Lídia; Frederico Aires da Silva. "Comunicação e Cultura Digital". Revista Comunicando 11 2 (2022): https://www.revistacomunicando.sopcom.pt.
    Published
Thesis / Dissertation
  1. André, Ana Filipa Santos. "Development of novel antibody-drug conjugated molecules for treatment of B-cell maligancies". PhD, 2023. http://hdl.handle.net/10400.5/29503.
  2. Henriques, Mafalda Alexandra Pereira. "Characterization of the humoral response of domestic cats naturally infected with SARS-CoV-2". Master, 2022. http://hdl.handle.net/10400.5/24985.
  3. Cardoso, Sara Alexandra Clemente. "SARS-CoV-2 Human-to-Animal Transmission Interface". Master, 2022. http://hdl.handle.net/10400.5/26569.
  4. Loureiro, Joana Teles. "In vitro evaluation of the cytotoxic potential of the immunotoxin Moxetumomab pasudotox for the treatment of canine lymphoma". Master, 2022. http://hdl.handle.net/10400.5/24464.
  5. Leonardo, Ana Sofia Rodrigues. "Development of dual-targeting immunoliposomes against canine B cell lymphoma". Master, 2022. http://hdl.handle.net/10400.5/26841.
  6. Santos, Filipe Miguel Barão dos. "Canine lymphoma immunophenotype prevalence and predisposition and evaluation of SN38 to be used in an antibody-drug conjugate for B-cell lymphoma treatment". Master, 2021. http://hdl.handle.net/10400.5/21061.
  7. Rocha, Pilar Carolina Carballal. "Study of glioblastoma occurrence in small animals and development of a drug delivery system by liposomes encapsulated with panobinostat". Master, 2021. http://hdl.handle.net/10400.5/21242.
  8. Rocha, Pilar Carolina Carballal. "Estudo de ocorrência de glioblastoma em pequenos animais e desenvolvimento de um sistema de entrega de fármacos por lipossomas encapsulados com panobinostat". Master, 2021. http://hdl.handle.net/10400.5/21242.
  9. Rita, Cristina Correia Costa. "Contribuição para o desenvolvimento de um imunolipossoma com duplo direcionamento ativo para o tratamento de tumores cerebrais". Master, 2020. http://hdl.handle.net/10400.5/20208.
  10. Moutinho, Isa Soraia Batista. "Desenvolvimento de anticorpos bi-específicos para o tratamento do VIH-1". Master, 2019. http://hdl.handle.net/10451/41710.
  11. Rico, Inês Casais e Costa. "Desenvolvimento e otimização de lipossomas encapsulados com citotóxicos de elevada potência para imunoterapia". Master, 2019. http://hdl.handle.net/10400.5/17334.
  12. Silva, Marta Lisete Santos da. "Development of a new immunoliposome drug delivery system for treatment of pneumococcal meningitis". Master, 2019. http://hdl.handle.net/10451/40166.
  13. Dias, Joana Nunes Ribeiro. "Clinical and immunological characterization of naturally occurring canine lymphoma : development and application of engineered recombinant antibodies for diagnosis and treatment". PhD, 2019. http://hdl.handle.net/10400.5/17735.
  14. Marques, Fabiana Carvalho. "Development of specific recombinant single-domain antibodies against gp120 HIV-1 glycoprotein and their selection by Phage Display". Master, 2018. http://hdl.handle.net/10400.5/16334.
  15. Freire, Miriam Monteiro. "Analysis of surface receptors in lymphocytes of canine patients with Lymphoma B". Master, 2018. http://hdl.handle.net/10400.5/15006.
  16. Almeida, André Gil Pedro Pinto de. "Characterization of canine lymphoma receptors for immunotherapy". Master, 2018. http://hdl.handle.net/10400.5/15822.
  17. Prata, Sara Azevedo. "Modelo canino para sépsis : contribuição para a classificação e estratificação em doentes sépticos". Master, 2017. http://hdl.handle.net/10400.5/14334.
  18. André, Ana Filipa Santos. "Selection of single domain antibodies against the CD20 receptor: a new treatment with potencial anti-tumor properties for B-cell malignancies". Master, 2016. http://hdl.handle.net/10451/25211.
  19. Cantante, Cátia Sofia de Carvalho, 1982-. "Engineering new strategies to improve pharmacokinetics of therapeutic proteins". PhD, 2014. http://hdl.handle.net/10451/12093.
  20. Canhão, Pedro Manuel Correia. "Selection of small domain antibodies specific for beta-amyloid peptide". Master, 2013. http://hdl.handle.net/10451/27690.
  21. Lourenço, Sara Ferreira Llorente Grancho, 1981-. "Selection and characterization of single-domain antibodies specific to HIV-1 gp41 protein". Master, 2011. http://hdl.handle.net/10451/4175.
  22. Silva, Frederico Nuno Castanheira Aires da, 1974-. "Recombinant intracellular antibodies for molecular gene therapy of HIV-1 infection". PhD, 2008. http://hdl.handle.net/10451/264.
  23. Silva, Frederico Nuno Castanheira Aires da. "Inhibition of human immunodeficiency virus replication by rabbit-derived VH single-domain intracellular antibodies". Master, Universidade de Aveiro UACOOPERA, 2004. http://hdl.handle.net/10773/17595.

Intellectual property

Provisional application for patent
  1. 2022. "Highly specific rabbit single-domain antibodies for drug delivery in immunotherapy applications. International Patent- PCT/IB2022/056303,".
  2. 2016. "Antibody molecules and peptide delivery systems for use in alzheimer's disease and related disorders - PCT/IB2016/050467".
    Protected
  3. 2016. "Cell-Based Methods for Coupling Protein Interactions and Binding Molecule Selection and Diversification - PCT/PT2014/000016".
    Protected
  4. 2013. "Dual Antigen-Binding Molecules and Uses Thereof - PCT 61/771546".
    Protected
  5. 2012. "Modified albumin-binding domains and uses thereof to improve pharmacokinetics - PCT/PT2012/000036".
    Protected
  6. 2012. "Anti-Tumor Necrosis Factor-Alpha Agents and Uses Thereof - PCT/PT2012/000035".
    Protected
  7. 2008. "Engineered rabbit antibody variable domains and uses thereof - CT/PT2008/000018".
Activities

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2021 - Current Development of potent and broadly neutralizing single-domain antibodies for SARS-CoV-2 treatment and prevention
Supervisor
 Veterinary Sciences (PhD)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2019 - 2023 DEVELOPMENT OF NOVEL ANTIBODY-DRUG CONJUGATED MOLECULES FOR TREATMENT OF B-CELL MALIGNANCIES
Supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2022 - 2022 DEVELOPMENT OF DUAL-TARGETING IMMUNOLIPOSOMES AGAINST CANINE B CELL LYMPHOMA
Supervisor
 Master’s degree in Veterinary Sciences (Master)
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2022 - 2022 INTERFACE OF TRANSMISSION OF SARS-COV-2 FROM HUMANS TO ANIMALS
Supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2022 - 2022 CHARACTERIZATION OF THE HUMORAL RESPONSE OF DOMESTIC CATS NATURALLY INFECTED WITH SARS-COV-2
Supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2022 - 2022 In vitro evaluation of the cytotoxic potential of the immunotoxin Moxetumomab Pasudotox for the treatment of canine lymphoma
Supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2021 - 2021 Canine lymphoma immunophenotype prevalence and predisposition: validation of SN38 to be used in an antibody-drug conjugate for b-cell lymphoma treatment
Supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2021 - 2021 Brain tumors: Study of the frequency in small animals and development of a drug delivery system by encapsulated liposomes with panobinostat
Supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2020 - 2020/12/20 Development of bispecific antibodies for treatment of HIV infection
Supervisor
Universidade de Lisboa Faculdade de Ciências, Portugal
2020 - 2020/10/29 Development of a new immunoliposome drug delivery system for treatment of pneumococcal meningites
Supervisor
Universidade de Lisboa Faculdade de Ciências, Portugal
2020 - 2020/06/16 Development of immunoliposomes for the transport of drugs to the brain
Supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2019 - 2019 Development and optimization of liposomes encapsulated with potent cytotoxic compounds for immunotherapy
Supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2018 - 2018 Development of specific recombinant single-domain antibodies against gp120 HIV-1 glycoprotein and their selection by Phage Display
Supervisor
Universidade de Lisboa Faculdade de Medicina Dentária, Portugal
2018 - 2018 Characterization of canine lymphoma receptors for immunotherapy
Supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2018 - 2018 Analysis of surface receptors in lymphocytes of canine patients with lymphoma b
Co-supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2014 - 2018 Clinical and immunological characterization of naturally occurring canine lymphoma: Development and application of engineered recombinant antibodies for diagnosis and treatment
Supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2017 - 2017 Canine model for sepsis: contribution for classification and stratification in patients with sepsis
Co-supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2017 - 2017 Immunological Characterization of Canine Lymphoma: evaluation of the expression of immune mediators
Co-supervisor
Universidade de Lisboa Faculdade de Medicina Veterinária, Portugal
2016 - 2016 Selection of single domain antibodies against the CD20 receptor: A new treatment with potential anti-tumor properties for B-cell malignances
Supervisor
Universidade de Lisboa Faculdade de Ciências, Portugal
2010 - 2014 Engineering new strategies to improve pharmacokinetics of therapeutic proteins
Co-supervisor
 Pharmaceutical sciences (PhD)
Associação da Faculdade de Farmácia para a Investigação e Desenvolvimento, Portugal
2013 - 2013 Selection of small domain antibodies against Aß42 for therapeutic applications in Alzheimer disease
Supervisor
Universidade de Lisboa Faculdade de Farmácia Biblioteca, Portugal
2011 - 2011 Selection and Characterization of single domain antibodies againts the HIV-1 gp41 protein and the HR1 domain
Supervisor
Universidade de Lisboa Faculdade de Medicina, Portugal
Distinctions

Other distinction

2022 Selected as Innovation Showcase Finalists 2022 - Emerging Start-Up
2022 Award for the best PhD work (Ana Andre) in the category of Fundamental Research awarded at the 5th Annual Conference of redeSAÚDE – University of Lisbon
Universidade de Lisboa, Portugal
2019 Best Presentation Prize at the World Pharm Congress
World Pharm Congress in Boston, United States
2016 2nd prize for the best start-up project (Joana Dias, PhD Student)
2012 Deloitte´s Technology fast 500 companies in the EMEA region (Europe, the Middle East, and Africa's). Prize awarded to the company Technophage while Frederico Silva had the functions of Scientific Unit Manager
2007 Best oral presentation - Biotecnology Simposium
2004 Dr. Champalimaud Prize - Basic Research in the HIV field
2003 Best Oral Presentation - Biotechnology Simposium