Margarida Archer Frazão (4A1E-BD99-92EB)

Curriculum Vitae

Margarida Archer Frazão

Margarida Archer graduated in Applied Chemistry (Biotechnology) at FCT-UNL in 1992 with one ERASMUS semester at University of Tuebingen. She received her PhD degree in Biochemistry from Universidade NOVA de Lisboa in 1999, during this time she collaborated with Nobel laureate R. Huber (Max Planck Institute of Biochemistry, Munique). Her main research interests are structural biology, structure and function of membrane-bound and health related proteins, structure-based drug design. She is Principal Investigator at ITQB NOVA and heads the Membrane Protein Crystallography group, currently with 1 junior scientist, 2 PhD students, 2 Master and 1 BSc fellows. She has supervised 10 PhD students (1 M. Curie and 1 MIT-Portugal fellows), 14 MSc students, 8 Post-Docs (two M. Curie fellows) and around 20 (under)graduate students. She has participated in 11 PhD and around 42 master juries. She published more than 60 articles in peer-reviewed journals and 9 book chapters, was invited for 24 oral communications in national and international meetings. She co-organized 6 international meetings/workshops at ITQB, was in the local organization of 10 international meetings and in the scientific committee of 3 international conferences. M. Archer currently co-coordinates national FCT project "Biocatalytic synthesis of oligoarabinosides for the study of drug targets and development of novel anti-tuberculosis drugs", is PI of InnoValley PoC "Computational engineered proteins for Flavivirus diagnostics and vaccine development”, and is Portuguese team leader in EU-H2020 project iNEXT-Discovery. She has been PI in 8 FCT and 1 FLAD projects, participated in 8 European projects (4 of which as Portuguese team leader and scientific board member in M. Curie ITN network -Structural Biology of Membrane Proteins), team member in 22 national projects (leader of structural biology task); being granted over 1.5 MEur. She is in the review editorial board of Frontiers in Structural Biology- Frontiers in Molecular Biosciences and Acta Crystallogr F, coordinator of Portuguese Instruct Research Site (ITQB node), national delegate of the European Crystallographic Association (ECA), national delegate on executive commission of Instruct-ERIC and scientific committee member (X-ray Crystallography) at the Portuguese Centre for Integrated Structural Biology (PCISBIO). She has participated in review panels for Swedish MAX-IV Synchrotron, ESRF Grenoble, European Science Foundation, BBSRC, Irish Research Council for Science, Engineering and Technology, and FCT.

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Identification

Personal identification

Full name: Margarida Archer Frazão

Author identifiers

Ciência ID: 4A1E-BD99-92EB

Education

	Degree	Classification
1999 - 2003 Concluded	Structure and function of proteins (Pós-doutoramento) Major in Structural Biology Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1999/03/02 Concluded	Bioquímica (Doutoramento) Major in Especialidade: Bioquímica Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal "Crystal Structure Analysis of Metalloproteins: Cytochrome C´ and Desulforedoxin (native and Metal Reconstituted)" (THESIS/DISSERTATION)
1992 Concluded	Química Aplicada (Licenciatura) Major in Biotecnologia Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal	17
1990 - 1991 Attended	Erasmus (Bachelor) Major in Biochemistry Eberhard Karls Universität Tübingen, Germany

Affiliation

Science

	Category Host institution	Employer
2020 - Current	Principal Investigator (Research)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2015 - 2020	Principal Investigator (Research)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2003/07 - 2014/12	Auxiliary Researcher (Research)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1993 - 1995	Research Trainee (Research)	Fundação para a Ciência e a Tecnologia, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1991 - 1992	Research Trainee (Research)	Fundação para a Ciência e a Tecnologia, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Teaching in Higher Education

	Category Host institution	Employer
2018 - Current	Visiting Teacher (Polytechnic Teacher)	Escola Superior de Saúde da Cruz Vermelha Portuguesa, Portugal
	Escola Superior de Saúde da Cruz Vermelha Portuguesa, Portugal
2013 - Current	Lecturer (University Teacher)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2009 - Current	Invited Assistant Professor (University Teacher)	Universidade Nova de Lisboa Faculdade de Ciências Médicas, Portugal

2000 - 2001	Invited Associate Professor (University Teacher)	Universidade Católica Portuguesa, Portugal
	Universidade Católica Portuguesa, Portugal
1990 - 1991	Assistant (University Teacher)	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
	Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Projects

Grant

	Designation	Funders
2023/06/01 - 2024/12/31	Computational engineered proteins for Flavivirus diagnostics and vaccine development IOVPoC-2022-08 Principal investigator Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal	OeirasValley Ongoing

Contract

	Designation	Funders
2021/01/01 - 2025/12/31	Ciências da Vida para um Futuro Saudável e Sustentável LA/P/0087/2020 10.54499/LA/P/0087/2020 Instituto Português de Oncologia de Lisboa Francisco Gentil EPE, Portugal Universidade Nova de Lisboa Molecular Structural and Cellular Microbiology, Portugal Universidade Nova de Lisboa, Portugal Instituto de Biologia Experimental e Tecnológica, Portugal Instituto Gulbenkian de Ciência, Portugal Universidade de Lisboa Centro de Estudos Comparatistas, Portugal BioResources 4 Sustainability, Portugal Universidade de Trás-os-Montes e Alto Douro Centro de Investigação e de Tecnologias Agro-Ambientais e Biologicas, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2021/03/01 - 2024/02/29	Biocatalytic synthesis of oligoarabinosides for the study of drug targets and development of novel anti-tuberculosis drugs PTDC/BIA-BQM/4056/2020 Universidade Nova de Lisboa, Portugal Universidade Nova de Lisboa Microbiologia Molecular Estrutural e Celular, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2019 - 2022	One World – one Health: Integrative approaches in Structural Biology and cryo-Electron Microscopy CYTED@ITQB Principal investigator Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Ciencia y Tecnología para el Desarrollo Ongoing
2019 - 2022	Infrastructure for transnational access and discovery in structural biology 871037 Researcher Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	European Commission Ongoing
2019 - 2022	Membrane protein integrated technologies development for drug design 823780 Researcher Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions
2018/10/01 - 2021/09/30	As ribonucleases como ferramentas para combater o patogéneo alimentar Campylobacter jejuni PTDC/BIA-BQM/28479/2017 Universidade Nova de Lisboa, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2018/10/01 - 2021/09/30	Desvendar a estrutura de proteínas membranares envolvidas na síntese da parede celular em micobactérias para desenvolver novos medicamentos anti-tuberculose e melhor compreender os mecanismos de resistência PTDC/BIA-BQM/30421/2017 Principal investigator Universidade Nova de Lisboa, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2018/10/01 - 2021/09/30	Doçuras ou Travessuras? Glico-conjugados Organometálicos de Ruténio(II) como Agentes Anticancerígenos Selectivos PTDC/MED-QUI/31468/2017 Universidade Nova de Lisboa, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2018/10/01 - 2021/09/30	Odisseia Metabolica em Staphylococcus aureus PTDC/BIA-BQM/28827/2017 Universidade Nova de Lisboa, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2016/07/01 - 2020/06/30	Pequenas moléculas inibidoras do proteassoma: um passo em frente na<br>descoberta de fármacos antitumorais PTDC/QEQ-MED/7042/2014 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal Universidade da Beira Interior, Portugal Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal	Fundação para a Ciência e a Tecnologia Ongoing
2017 - 2020	Releasing the full potential of Instruct to expand and consolidate infrastructure services for integrated structural biology 731005 Researcher Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	European Commission Concluded
2019/01/01 - 2019/12/31	iNOVA4Health - Programa de Medicina Translacional (iBET, CEDOC/FCM, IPOLFG e ITQB) UID/Multi/04462/2019 Instituto Português de Oncologia de Lisboa Francisco Gentil EPE, Portugal Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal Universidade Nova de Lisboa, Portugal Universidade Nova de Lisboa Centro de Estudos de Doenças Crónicas, Portugal Universidade Nova de Lisboa Medical School, Portugal Instituto de Biologia Experimental e Tecnológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2016/04/20 - 2019/10/19	Inibição da sulfidogénese microbiana: Caracterização bioquímica e estrutural da DsrD PTDC/BIA-MIC/6512/2014 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal BioResources 4 Sustainability, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2018 - 2019	Structural Characterization of Highly-Specific Engineered Zika Virus Antigens Complexed with Human Monoclonal Antibodies 502 Principal investigator Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	European Commission Concluded
2015 - 2018	Infrastructure for NMR, EM and X-ray Crystallography for translational research iNEXT@ITQB Researcher Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	EU Framework Programme for Research and Innovation Euratom Concluded
2013/07/01 - 2016/01/31	Interacção entre Legionella pneumophila e o citosqueleto de actina da célula hospedeira PTDC/BIA-MIC/2821/2012 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal Associação para a Inovação e Desenvolvimento da FCT, Portugal Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2015 - 2016	Tailored ionic liquids to boost membrane protein solubilisation and crystallization INSTRUCT@ITQB Principal investigator Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	European Commission Concluded
2013/05/01 - 2015/10/31	Investigação estrutural da sintase de Fosfatidilinositol fosfato, um alvo terapêutico para a Tuberculose PTDC/BBB-BEP/2532/2012 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2012/03/01 - 2015/08/31	Biologia estrutural de Quinases de Histidina: um alvo para o desenvolvimento de novos fármacos antibacterianos PTDC/BIA-PRO/118535/2010 Principal investigator Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010/01/01 - 2013/10/31	Projecto Estrutural sobre a Metalómica de Deinococcus radiodurans PTDC/BIA-PRO/100365/2008 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010/04/01 - 2013/09/30	Regulação da sinaptogénese pela quinase Cdk5 e Shank3: Estudo Bioquímico e Estrutural PTDC/SAU-NEU/103720/2008 Principal investigator Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010/04/01 - 2013/09/30	Estudo de um metabolismo energético ancestral: a redução dissimilativa de sulfito PTDC/QUI-BIQ/100591/2008 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010/01/01 - 2013/06/30	Biologia estrutural de transportadores membranares de Arquea PTDC/BIA-PRO/103718/2008 Principal investigator Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010/01/01 - 2013/06/30	Structural and functional investigation of type II NADH:quinone oxidoreductases. PTDC/BIA-PRO/100288/2008 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2010/01/01 - 2012/12/31	PneumoCaPTS - Regulação de factores de virulência por repressão catabólica mediada por glucose PTDC/BIA-MIC/099963/2008 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2009/01/01 - 2012/06/30	Marcação específica de proteínas in vitro, in vivo e à superfície das células por um novo tipo de transglutaminase isolada do esporo de Bacillus subtilis PTDC/BIO/73946/2006 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2008/05/05 - 2012/04/30	Estudo dos mecanismos de adaptação a stress térmico em microorganismos hipertermófilos: função fisiológica de poliolfosfodiésteres PTDC/BIA-MIC/71146/2006 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2007/11/02 - 2011/10/31	A reactividade química como ferramenta no desenho de fármacos: modificação do núcleo de beta-lactama para melhorar a inibição de proteases de serina PTDC/QUI/64056/2006 Universidade de Lisboa Faculdade de Farmácia, Portugal Universidade de Lisboa Faculdade de Farmácia, Portugal Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2008/01/01 - 2011/07/30	Procura do mínimo denominador comum funcional e estrutural das reductase do oxigénio do tipo hemo-cobre. PTDC/QUI/66559/2006 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2008/04/01 - 2011/03/31	Investigação do mecanismo de transdução de energia, ciclo-Q em complexos da cadeia respiratória PTDC/BIA-PRO/66557/2006 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2008/01/01 - 2011/02/28	Oxidação de quinois por citocromos c membranares envolvidos na respiração bacteriana - estudos estruturais do complexo modelo NrfHA PTDC/BIA-PRO/68486/2006 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2007/10/01 - 2011/01/31	Z-DNA e Z-RNA: o longo caminho para uma função biológica PTDC/SAU-MII/69084/2006 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2008 - 2011	Structural Biology of Membrane Proteins PITN-GA-2008-211800 Researcher Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	European Commission Seventh Framework Programme for Research and Technological Development Concluded
2008/01/01 - 2009/12/31	Genómica estrutural aplicada a proteínas transportadoras de membrana de Archaea PTDC/BIA-PRO/66833/2006 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2004 - 2009	European Macromolecular Crystallography Infrastructure Network 2 505977 Researcher Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	European Commission Sixth Framework Programme Concluded
2004 - 2009	European Membrane Protein Consortium EMeP@ITQB Researcher Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	European Commission Sixth Framework Programme Concluded
2005/09/01 - 2008/03/31	Estudos estruturais e funcionais de receptores Toll-like: Activação da resposta imunitária inata POCI/SAU-IMI/55729/2004 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal Fundação Calouste Gulbenkian, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2006/01/01 - 2007/12/31	Papel da interação das proteínas AgfA e AgfB de Salmonella enterica serovar Typhimurium na polimerização superficial das fibras amilóides que constituem as fimbrias agregativas. POCI/SAU-IMI/55520/2004 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2005/07/01 - 2007/06/30	Estudos da interacção quinona-proteína em complexos de cadeias respiratórias POCI/QUI/59824/2004 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2005/06/01 - 2007/06/30	Caracterização estrutural de proteínas membranares da cadeia respiratória de um organismo termoacidofílico POCI/BIA-PRO/55621/2004 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2005/05/16 - 2007/06/30	Mechanisms of repression by AraR, a key regulator of carbohydrates utilization in Bacillus subtilis POCI/BIA-MIC/61140/2004 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded
2003/06/01 - 2006/11/30	Análise estrutural de complexos de metiltransferase com inibidores para o desenvolvimento de novas drogas POCTI/BME/38306/2001 Principal investigator	Fundação para a Ciência e a Tecnologia Concluded
2001/03/01 - 2004/08/31	Estrutura e função de elicitinas e seu papel na infecção do sobreiro (Quercus suber) pelo fungo Phytophthora cinnamomi POCTI/BME/34701/2000	Fundação para a Ciência e a Tecnologia Concluded
1995/03/01 - 1999/02/28	ANÁLISE CRISTALOGRÁFICA DE PROTEÍNAS CONTENDO CENTROS DE FERRO E MOLIBDÉNIO PRAXIS XXI/BD/2795/94 Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal	Fundação para a Ciência e a Tecnologia Concluded

Outputs

Publications

Book	Moraes, I.; Archer, M.. Methods for the successful crystallization of membrane proteins. 2015. 10.1007/978-1-4939-2230-7_12 Brito, J.A.; Archer, M.. X-ray Crystallography. 2013. 10.1016/B978-0-444-56351-4.00009-9 Romão, M.J.; Archer, M.. Structural versatility of proteins containing rubredoxin-type centers. 2008. 10.1002/9783527613700.ch20
Book chapter	Brito, José A.; Archer, Margarida. "Structural biology techniques: X-ray crystallography, cryo-electron microscopy, and small-angle X-ray scattering". In Practical Approaches to Biological Inorganic Chemistry, 375-416. Elsevier, 2020. 10.1016/b978-0-444-64225-7.00010-9 Moraes, Isabel; Archer, Margarida. "Methods for the Successful Crystallization of Membrane Proteins". In Methods in Molecular Biology, 211-230. Springer New York, 2014. 10.1007/978-1-4939-2230-7_12 Romão, Célia V.; Archer, Margarida; Lobo, Susana A.; Louro, Ricardo O.; Pereira, Inês A. C.; Saraiva, Lígia M.; Teixeira, Miguel; Matias, Pedro M.. "89 Diversity of Heme Proteins in Sulfate Reducing Bacteria". In Handbook of Porphyrin Science (Volume 19), 139-229. World Scientific Publishing Company, 2012. 10.1142/9789814335508_0016 Romao, Celia V; Archer, Margarida; Lobo, Susana A.; Louro, Ricardo O.; Pereira, Ines A.C.; Saraiva, Ligia M.; Teixeira, Miguel; Matias, Pedro M.. "Diversity of Heme Proteins in Sulfate Reducing Bacteria". In Handbook of Porphyrin Science (Volume 19), edited by Smith K.M.; Guilard R.; Kadish K.M., 139-230. Singapore: World Scientific Publishing Company, 2012. Published
Conference poster	Almeida, Vanessa T.; Conceição, Cristiano; Zinkle, Allen; Liu, Yaqi; Issoglio, FM; Rodrigues, José; Brito, José A.; et al. Corresponding author: Almeida, Vanessa T.. "AftD as a new potential drug target to fight tuberculosis". Paper presented in 13th ITQB NOVA PhD Students Meeting, 2023. Almeida, Vanessa T.; Issoglio, FM; Rodrigues, José; Archer, Margarida. Corresponding author: Almeida, Vanessa T.. "Structure-based virtual screen for potential AftD inhibitors using a drug repurposing strategy". Paper presented in 2nd National Crystallographic Meeting, 2022. Archer, Margarida; Rodrigues, José; Almeida, Vanessa T.; Tan, Yong Zi; Kloss, Brian; Potter, CS; Carragher, B; Mancia, Filippo. Corresponding author: Archer, Margarida. "Structural analysis of arabinofuranosyltransferases, key players in mycobacteria cell wall synthesis". Paper presented in COMPPÅ Symposium on Membrane Protein Production and Analysis, 2022. Gonçalves, Juliana; Juliano, A. Margarida; Charepe, Nádia; Alenquer, Marta; Athayde, Diogo; Ferreira, Filipe; Archer, Margarida; et al. "Secretory IgA and T cells targeting SARS-COV-2 spike protei are transferred to the breastmilk upon mRNA vaccination". Paper presented in Nature Conference: Understanding COVID-19 to prepare for the nex pandemic, 2022. Conceição, Cristiano; Issoglio, FM; Almeida, Vanessa T.; Rodrigues, José; Archer, Margarida; M. Rita Ventura. Corresponding author: Conceição, Cristiano. "Development of a new functional assay to study potential anti-tuberculosis drug targets - Arabinofuranosyltransferases". Paper presented in 12th ITQB NOVA PhD Students Meeting, 2022. Ferreira, Inês; Casalou, Cristina; Nobre, Lígia; Athayde, Diogo; Silva, Sofia; Bandeiras, Tiago; Archer, Margarida; Barral, Duarte. "Impairing tumor progression through the inactivation of the GTP-binding protein Arl13b". Paper presented in SINAL 2018 - 9th Meeting on Signal Transduction, 2018.
Journal article	Bárria, Cátia; Athayde, Diogo; Hernandez, Guillem; Fonseca, Leonor; Casinhas, Jorge; Cordeiro, Tiago N.; Archer, Margarida; et al. "Structure and function of Campylobacter jejuni polynucleotide phosphorylase (PNPase): Insights into the role of this RNase in pathogenicity". Biochimie 216 (2024): 56-70. http://dx.doi.org/10.1016/j.biochi.2023.10.006. 10.1016/j.biochi.2023.10.006 Moreno-Cabezuelo, José Ángel; del Carmen Muñoz-Marín, María; López-Lozano, Antonio; Athayde, Diogo; Simón-García, Ana; Díez, Jesús; Archer, Margarida; Issoglio, Federico M.; García-Fernández, José Manuel. "Production, homology modeling and mutagenesis studies on GlcH glucose transporter from Prochlorococcus sp. strain SS120". Biochimica et Biophysica Acta (BBA) - Bioenergetics 1864 2 (2023): 148954. http://dx.doi.org/10.1016/j.bbabio.2022.148954. Published • 10.1016/j.bbabio.2022.148954 Márcia M. S. Alves; Manuel N. Melo; Haydyn D. T. Mertens; Ana B. Pereiro; Margarida Archer. "Studies on the Interaction between Model Proteins and Fluorinated Ionic Liquids". Pharmaceutics (2023): https://doi.org/10.3390/pharmaceutics15010157. 10.3390/pharmaceutics15010157 Isabelle F. T. Viana; Carlos H. B. Cruz; Diogo Athayde; W. Camilla S. Adan; Lícya S. S. Xavier; Margarida Archer; Roberto D. Lins. "In vitro neutralisation of Zika virus by an engineered protein targeting the viral envelope fusion loop". Molecular Systems Design & Engineering (2023): https://doi.org/10.1039/D2ME00170E. 10.1039/D2ME00170E Márcia M. S. Alves; Paula Leandro; Haydyn D. T. Mertens; Ana B. Pereiro; Margarida Archer. "Impact of Fluorinated Ionic Liquids on Human Phenylalanine Hydroxylase—A Potential Drug Delivery System". Nanomaterials (2022): https://doi.org/10.3390/nano12060893. 10.3390/nano12060893 Juliana Gonçalves; A. Margarida Juliano; Nádia Charepe; Marta Alenquer; Diogo Athayde; Filipe Ferreira; Margarida Archer; et al. "Secretory IgA and T cells targeting SARS-CoV-2 spike protein are transferred to the breastmilk upon mRNA vaccination". Cell Reports Medicine (2021): https://doi.org/10.1016/j.xcrm.2021.100468. 10.1016/j.xcrm.2021.100468 José Rodrigues; Vanessa T. Almeida; Ana L. Rosário; Yong Zi Tan; Brian Kloss; Filippo Mancia; Margarida Archer. "High Throughput Expression Screening of Arabinofuranosyltransferases from Mycobacteria". Processes 9 4 (2021): 629-629. https://doi.org/10.3390/pr9040629. 10.3390/pr9040629 Alves, Márcia M.S.; Araújo, João M.M.; Martins, Ivo C.; Pereiro, Ana B.; Archer, Margarida. Corresponding author: Archer, Margarida. "Insights into the interaction of Bovine Serum Albumin with Surface-Active Ionic Liquids in aqueous solution". Journal of Molecular Liquids 322 (2021): 114537-114546. http://dx.doi.org/10.1016/j.molliq.2020.114537. Published • 10.1016/j.molliq.2020.114537 Magoch, Malgorzata; Nogly, Przemyslaw; Grudnik, Przemyslaw; Ma, Pikyee; Boczkus, Bozena; Neves, Ana Rute; Archer, Margarida; Dubin, Grzegorz. "Crystal Structure of Mannose Specific IIA Subunit of Phosphotransferase System from Streptococcus pneumoniae". Molecules 25 20 (2020): 4633. http://dx.doi.org/10.3390/molecules25204633. 10.3390/molecules25204633 Tan, Yong Zi; Rodrigues, José; Keener, James E.; Zheng, Ruixiang Blake; Brunton, Richard; Kloss, Brian; Giacometti, Sabrina I.; et al. "Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis". Nature Communications 11 1 (2020): http://dx.doi.org/10.1038/s41467-020-17202-8. 10.1038/s41467-020-17202-8 Tan, Yong Zi; Zhang, Lei; Rodrigues, José; Zheng, Ruixiang Blake; Giacometti, Sabrina I.; Rosário, Ana L.; Kloss, Brian; et al. "Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria". 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Oral presentation

	Presentation title	Event name Host (Event location)
2021/10/13	ProMeTeus, a RISE-MSCA Staff exchange program. Challenges and opportunities in biomedicine and biotechnology	Vallone B (Sapienza University of Rome, Italy), ProMeTeus, a RISE-MSCA Staff exchange program. Challenges and opportunities in biomedicine and biotechnology Margarida Archer (Oeiras, Portugal)
2021/07/24	Membrane glycosyltransferases as promising anti-tuberculosis targets. -hybrid conference, virtual participation	13th European Biophysics Conference EBSA2021 EBSA (Vienna, Austria)
2021/06/28	Instruct-PT presentation	Instruct Centres General Forum Meeting Instruct ERIC, Oxford (United Kingdom)
2021/06/15	Accomplishments within Instruct-Ultra	PCISBIO webinar - online PCISBIO (Portugal)
2019	Is it just a glycosyltransferase?! That´s the big question in the intricate cell wall of mycobacteria	4th Annual Users Meeting of iNEXT iNEXT (Lund, Sweden)
2019	Cryo-EM structure of glycosyltransferase AftD involved in mycobacterial cell wall synthesis	I National Crystallographic Meeting ANURS (Aveiro, Portugal)
2019	What X-ray Crystallography is the 3D EM structure of mycobacterial glycosyltransferase telling us?	Seminar at Center for Chronic Diseases Nova Medical School (Lisboa, Portugal)
2018	How can structure help understanding function? The story of arabinofuranosyltransferase and its role in the intricate cell wall synthesis of mycobacteria	SPBf conferences Universidade do Minho (Braga, Portugal)
2017	Unraveling the structure of molecular machines at atomic level - Crystal structure of a membrane protein from the CDP-alcohol phosphatidyltransferase family	Seminar at Biological Sciences & Bioengineering Indian Institute of Technology (Kanpur, India)
2015	Turning adversity into opportunity, the rocky road leading to membrane protein structure	Physiology and Cellular Biophysics Seminar Series Columbia University Medical Center (Nova Iorque, United States)
2014	The rock road of membrane proteins: from gene to structure	International Year of Crystallography - commemorative seminar FCT NOVA (Setúbal, Portugal)
2013	Structure of a new membrane protein	Benzon Symposium - (Copenhaga, Denmark)
2013	X-ray structure of bifunctional enzyme cytidylyltransferase/CDP-alcohol phosphatidyltransferase from Archaeoglobus fulgidus at 2.65 Å	- Faculdade de Ciências da Universidade de Lisboa (Lisboa, Portugal)
2009	Structure of cytochrome c quinol dehydrogenase NrfH	1st International Multidisciplinary Workshop on Membrane Biology Frontiers – Dynamics, Energy, Structure and Technology - (Mykonos, Greece)
2009	Crystallography: Anatomy of life at atomic level	Encontro Ciência 2009 Fundação Calouste Gulbenkian (Lisboa, Portugal)
2008	Mechanism of sulfate reduction based on the crystal structure of a dissimilatory sulfite reductase bound to DsrC	1st Portuguese-Spanish-British joint Biophysics Congress Sociedade Portuguesa de Biofísica (Lisboa, Portugal)
2008	Structure-based drug design	Biotechnology Conference “Um saber Português como Saber do Mundo” Universidade Lusófona de Humanidades e Tecnologias (Lisboa, Portugal)
2007	Structural characterization of the membrane-anchored quinol dehydrogenase and its partner nitrite reductase NrfA	Seminar Series Astury Centre for Structural Molecular Biology (Leeds, United Kingdom)
2007	X-ray structure of the membrane-bound cytochrome c nitrite reductase complex NrfHA	International workshop on the Structural Proteomics of Membrane Proteins - (Rauischholzhausen, Germany)
2005	Protein Crystallography - Applications for pharmaceutical industry	III National Biochemistry Symposium Universidade da Beira Interior (Covilhã, Portugal)
2001	X-ray structure of catechol-O-methyltransferase complexed with a new tight-binding inhibitor	FEBS Forum of Young Scientists “Protein Structure-Function, Trafficking and Signalling Federation of European Biochemical Societies (Oeiras, Portugal)
2000	Crystal structure of b-cinnamomin, a toxic elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomi	30th course “Methods in Macromolecular Crystallography” International School of Crystallography (Erice, Italy)

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2022 - 2025	PhD thesis: Structure-based drug design to fight tuberculosis and understand drug resistance mechanisms Supervisor of Vanessa Almeida	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
2021 - 2024	PhD thesis: Structure-based design and synthesis of novel potential anti-tuberculosis drugs Co-supervisor of Cristiano Conceição	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
2019 - 2024	PhD thesis: Unravelling the molecular and structural basis of Zika Virus antibody neutralization Supervisor of Diogo Athayde	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
2017 - 2024	PhD thesis: Structural and functional studies of membrane enzymes involved in the synthesis of mycobacteria cell wall Supervisor of José Rodrigues	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2022 - 2023	MSc thesis: Studies on mycobacteria glycosyltransferases, promising targets to develop antituberculosis drugs Supervisor of Bárbara Rodrigues	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica António Xavier, Portugal
2017 - 2022	PhD thesis: The potential use of ionic liquids as drug delivery systems of therapeutic proteins Supervisor of Márcia Alves	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2018 - 2019	MSc thesis: Structure characterization of serine protease complexes with novel inhibitors Supervisor of Vanessa Almeida	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2017 - 2018	MSc thesis: Structural analysis of lactamase in complex with inhibitors Co-supervisor of Paulo Mamede	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2015 - 2016	MSc thesis: Structural studies of proteins involved in sulfur metabolism, thiosulfate dehydrogenase Supervisor of Maria Cordeiro	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2015 - 2016	MSc thesis: Functional and strutural analysis on proteins involved in sulfur metabolism Co-supervisor of André Gutierres	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2014 - 2016	MSc thesis: Production and characterization of membrane transporters Supervisor	Universidade do Minho, Portugal
2014 - 2015	MSc thesis: Structural and functional studies of Histidine sensor kinases Supervisor of Alina Kulakova	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2009 - 2013	PhD thesis: Crystallographic studies on membrane and cytoplasmic enzymes: Bifunctional cytidylyltransferase/CDP-alcohol phosphatidyltransferase, and a-phosphoglucomutase Supervisor of Przemyslaw Nogly	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2009 - 2013	PhD thesis: Functional and structural studies of two enzymes: membrane bound kinase and Z-DNA/Z-RNA-binding protein Supervisor of Ana Lúcia Rosário	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2007 - 2011	PhD thesis: Crystallographic studies on two hyperthermophilic enzymes Supervisor of José Brito	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2006 - 2011	PhD thesis: Crystallographic and Biochemical studies on Dissimilatory Sulfite Reductases Supervisor of Tânia Oliveira	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2009 - 2010	PhD thesis: Expression of Archeal Membrane Transporters and Structural Analysis of Protease – Inhibitor Complexes Supervisor of Stefan Hofbauer	Universität für Bodenkultur Wien Department für Integrative Biologie und Biodiversitätsforschung, Austria
2004 - 2008	PhD thesis: Crystallographic studies on the human protein ADAR1 and a transglutaminase from Bacillus subtilis Co-supervisor of Diana Plácido	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2001 - 2005	PhD thesis: Crystallographic studies on a-cinnamomin, catechol-O-methyltransferase and cytochrome c nitrite reductase Supervisor of Maria Luísa Rodrigues	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
1998 - 1999	PhD thesis: Structural characterization of desulforedoxin Supervisor of Ana Lúcia Carvalho	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal

Event organisation

	Event name Type of event (Role)	Institution / Organization
2021/02/16 - Current	iNEXT-Discovery 1st Annual Scientific Meeting – online, NKI, Amsterdam, Netherlands (2021/02/16) Symposium (President of the Organising Committee)
2021/01/20 - Current	Microbes (Cyted) Annual Consortium Meeting – online, ITQB, Oeiras, Portugal (2021/01/20) Meeting (Co-organisor)
2016 - 2016	CombStruct2016: Integrating X-ray crystallography and scattering with electron microscopy (2016 - 2016) Workshop (Member of the Organising Committee)
2013 - 2013	Structure and Function of Membrane Proteins: a multidisciplinary approach (2013 - 2013) Workshop (Member of the Organising Committee)
2009 - 2009	Annual meeting of the Marie Curie Integrated Training Network “Structural Biology of Membrane Proteins” (2009 - 2009) Meeting (Member of the Organising Committee)
2006 - 2006	Fundamentals of modern methods in Biocrystallography (2006 - 2006) Other (Member of the Organising Committee)
2006 - 2006	Hands-on Science (2006 - 2006) Conference (Member of the Scientific Committee)
2004 - 2006	Fundamentals of modern methods in Biocrystallography (2004 - 2004) Seminar (Member of the Organising Committee)	Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2005 - 2005	Membrane Proteins: the rocky road from gene to structure (2005 - 2005) Workshop (Member of the Organising Committee)
2001 - 2001	FEBS Forum of Young Scientists “Protein Structure-Function, Trafficking and Signalling (2001 - 2001) Symposium (Member of the Organising Committee)	Federation of European Biochemical Societies, United Kingdom
2001 - 2001	27th Meeting of the Federation of European Biochemical Societies (2001 - 2001) Congress (Member of the Organising Committee)	Federation of European Biochemical Societies, United Kingdom
1997 - 1997	17th European Crystallographic Meeting (ECM-17) (1997 - 1997) Congress (Member of the Organising Committee)

Committee member

	Activity description Role	Institution / Organization
2023/09/01 - Current	Monitoring Committee and Support Office for Students with Special Educational Needs of the New University of Lisbon Member

Conference scientific committee

	Conference name	Conference host
2023/01/01 - 2024/06/01	Instruct Biennial Structural Biology Conference (IBSBC): New Directions for Structural Biology	Instruct ERIC
2019/05/22 - 2019/05/24	4th Instruct Biennial Structural Biology Conference: : Deeper into the Cell

Consulting

	Activity description	Institution / Organization
2018/10/06 - Current	Member of Joint Project Committee, Merck and iBET

Interview (newspaper / magazine)

	Activity description	Newspaper / Forum
2021/12/03	Article on National Newspaper about the immunological protection against SARS-CoV2 in breast milk	Público Newspaper
2020/05/25	Interview related with the structural elucidation of Arabinofuranosyltransferases from Mycobacteria, a potential druggable target against tuberculosis	Público Newspaper

Journal scientific committee

	Journal title (ISSN)	Publisher
2021/06/20 - Current	• Review Editor in Frontiers in Medical Technology - Pharmaceutical Innovation
2014 - Current	Frontiers in Molecular Biosciences (2296-889X)	Frontiers Media SA
2012 - Current	Acta Crystallographica Section F Structural Biology and Crystallization Communications (1744-3091)	International Union of Crystallography

Distinctions

Award

1995	“Fundação Marquês de Pombal 95” - "Oeiras, A Ciência e o Desenvolvimento" Câmara Municipal de Oeiras, Portugal
1995	Crystal Award Max-Planck-Institut für Biochemie, Germany

Other distinction

1997	Best poster 4th FGIPS Meeting in Inorganic Chemistry, Corfu, Greece, Greece