Personal identification

Full name

Carolina Maria Balão da Silva

Citation names

• Silva, Carolina

Author identifiers

Ciência ID

4B1D-3FBA-EEE8

ORCID iD

0000-0002-9230-3919

Websites

• https://esbe.ipportalegre.pt/pt/ (Scholar)

Knowledge fields

• Agrarian Sciences - Veterinary Sciences

Languages

Language	Speaking	Reading	Writing	Listening	Peer-review
Spanish; Castilian	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
English	Advanced (C1)	Advanced (C1)	Advanced (C1)	Advanced (C1)
Italian	Beginner (A1)	Beginner (A1)	Beginner (A1)	Beginner (A1)

	Degree	Classification
2013 - 2014 Concluded	Curso de Especialista Universitario en Reproducción Asistida (Postgraduate Certificate) Universidad de Extremadura, Spain
2014 Concluded	n/a (Doutoramento) Universidad de Extremadura, Spain "Sex preselection in horses: identification of limiting factors" (THESIS/DISSERTATION)	Sobresaliente cum laude (10/10)
2011 Concluded	n/a (Magister) Universidad de Extremadura, Spain "Efecto de la melatonina como reductora de la peroxidación lipídica" (THESIS/DISSERTATION)	Sobresaliente (9/10)

Teaching in Higher Education

	Category Host institution	Employer
2014/08/01 - 2015/02/01	Invited Assistant Professor (University Teacher)	Universidad Agraria del Ecuador, Ecuador

Contract

	Designation	Funders
2022 - 2026	Regulación del paso de glucólisis a la ruta de las pentosas fosfato: Reprogramación metabólica como estrategia de conservación de semen equino PID2021-122OB-I00 Researcher Universidad de Extremadura Facultad de Veterinaria, Spain	España Ministerio de Ciencia e Innovación Ongoing
2020/11/01 - 2022/10/31	EQUIMAIS - Melhor produção equina ALT20-03-0246-FEDER-000055 Researcher Universidade de Évora, Portugal	Ongoing
2018 - 2022	Regulacion redox en el espermatozoide de equino: podemos hacer que las especies reactivas de oxigeno trabajen en nuestro favor durante la congelación y refrigeración? AGL2017-83149-R Researcher Universidad de Extremadura Facultad de Veterinaria, Spain	Ongoing
2017/01 - 2019/12/31	ALT Biotech RepGen: Recursos Genéticos Animais eBiotecnologias: projeção para o futuro ALT20-03-0246-FEDER- 000021 Researcher Instituto Nacional de Investigação Agrária e Veterinária, Portugal	Concluded
2017/09/13 - 2019/09/13	RUMMUNITY - Diminuição da mortalidade e morbilidade perinatais por falha da transferência da imunidade passiva em bovinos em regime extensivo com base na administração de plasma POCI-01-0145-FEDER-023757 Researcher Instituto Politécnico de Portalegre, Portugal	Concluded

Publications

Journal article

Carolina Balão da Silva; Elvira Matilla Pinto. "Efficacy validation of a low-cost handmade simulator (SIMCA-COW) in palpation, ultrasonography evaluation, and artificial insemination in cows". Veterinary World (2023): https://doi.org/10.14202/vetworld.2023.144-148. 10.14202/vetworld.2023.144-148 Carolina Balao Silva; Lina Costa; Rute Santos; Luisa Pereira; Henrique Pinto; Helga Waap. "Assessing the failure in transfer of passive immunity in beef calves: two case reports". Millenium - Journal of Education Technologi (2020): n.º 12-n.º 12. https://revistas.rcaap.pt/millenium/article/view/18963. 10.29352/MILL0212.09.00270 Silva, Carolina. "Mortality and Morbidity of Beef Calves in Free-Range Farms in Alentejo, Portugal—A Preliminary Study". Veterinary Medicine International (2019): http://dx.doi.org/10.1155/2019/3616284. 10.1155/2019/3616284 da Silva, C.B.; Gordo, L.G.; Cuesta Gerveno, J.M.; Ortega-Ferrusola, C.; Martín-Muñoz, P.; Carrasco, F.J.D.; Parejo, C.; Vega, F.P.. "A rare case of a primary unilateral low-grade paratesticular leiomyosarcoma in a 2 years old dog". Frontiers in Veterinary Science 6 MAR (2019): http://www.scopus.com/inward/record.url?eid=2-s2.0-85065440012&partnerID=MN8TOARS. 10.3389/fvets.2019.00083 Ortiz-Rodriguez, J.M.; Balao da Silva, C.; Masot, J.; Redondo, E.; Gazquez, A.; Tapia, J.A.; Gil, C.; Ortega-Ferrusola, C.; Peña, F.J.. "Rosiglitazone in the thawing medium improves mitochondrial function in stallion spermatozoa through regulating Akt phosphorylation and reduction of caspase 3". PloS one 14 7 (2019): e0211994-e0211994. http://www.scopus.com/inward/record.url?eid=2-s2.0-85069267935&partnerID=MN8TOARS. 10.1371/journal.pone.0211994 Ortega Ferrusola C; Martin Muñoz P; Ortiz-Rodriguez JM; Anel-López L; Balao da Silva C; Álvarez M; de Paz P; et al. "Depletion of thiols leads to redox deregulation, production of 4-hydroxinonenal and sperm senescence: a possible role for GSH regulation in spermatozoa.". Biology of reproduction (2018): http://europepmc.org/abstract/med/30418487. 10.1093/biolre/ioy241 Ortega Ferrusola C; Anel-López L; Ortiz-Rodriguez JM; Martin Muñoz P; Alvarez M; de Paz P; Masot J; et al. "Stallion spermatozoa surviving freezing and thawing experience membrane depolarization and increased intracellular Na.". Andrology (2017): http://europepmc.org/abstract/med/28973824. 10.1111/andr.12419 Martín Muñoz P; Anel-López L; Ortiz-Rodríguez JM; Álvarez M; de Paz P; Balao da Silva C; Rodríguez Martinez H; et al. "Redox cycling induces spermptosis and necrosis in stallion spermatozoa while the hydroxyl radical (OH•) only induces spermptosis.". Reproduction in domestic animals = Zuchthygiene (2017): http://europepmc.org/abstract/med/28833663. 10.1111/rda.13052 Balao da Silva, C.M.; Ortega-Ferrusola, C.; Morrell, J.M.; Rodriguez Martínez, H.; Peña, F.J.. "Flow Cytometric Chromosomal Sex Sorting of Stallion Spermatozoa Induces Oxidative Stress on Mitochondria and Genomic DNA". Reproduction in Domestic Animals 51 1 (2016): 18-25. http://www.scopus.com/inward/record.url?eid=2-s2.0-84955212288&partnerID=MN8TOARS. 10.1111/rda.12640 Davila, M.P.; Muñoz, P.M.; Bolaños, J.M.G.; Stout, T.A.E.; Gadella, B.M.; Tapia, J.A.; Balao Da Silva, C.; Ortega Ferrusola, C.; Peña, F.J.. "Mitochondrial ATP is required for the maintenance of membrane integrity in stallion spermatozoa, whereas motility requires both glycolysis and oxidative phosphorylation". Reproduction 152 6 (2016): 683-694. http://www.scopus.com/inward/record.url?eid=2-s2.0-84996899145&partnerID=MN8TOARS. 10.1530/REP-16-0409 Silva, Carolina. "Inhibition of Mitochondrial Complex I Leads to Decreased Motility and Membrane Integrity Related to Increased Hydrogen Peroxide and Reduced ATP Production, while the Inhibition of Glycolysis Has Less Impact on Sperm Motility". PLoS One (2015): 10.1371/journal.pone.0138777 Peña, F.J.; Plaza Davila, M.; Ball, B.; Squires, E.L.; Martin Muñoz, P.; Ortega Ferrusola, C.; Balao da Silva, C.. "The Impact of Reproductive Technologies on Stallion Mitochondrial Function". Reproduction in Domestic Animals 50 4 (2015): 529-537. http://www.scopus.com/inward/record.url?eid=2-s2.0-84936986302&partnerID=MN8TOARS. 10.1111/rda.12551 Gracia-Calvo, L.A.; Duque, J.; Balao da Silva, C.; Ezquerra, J.; Ortega-Ferrusola, C.. "Testicular perfusion after standing laparoscopic peritoneal flap hernioplasty in stallions". Theriogenology 84 5 (2015): 797-804. http://www.scopus.com/inward/record.url?eid=2-s2.0-84938990195&partnerID=MN8TOARS. 10.1016/j.theriogenology.2015.05.014 Morillo Rodriguez, A.; Plaza Dávila, M.; Miró Morán, A.; Martin Muñoz, P.; Balao da Silva, C.; Gallardo Bolaños, J.M.; Ortega Ferrusola, C.; et al. "Tumor Necrosis Factor a Phosphorylates c-Jun N-Terminal Kinase in Stallion Spermatozoa: Effect of Cryopreservation". Journal of Equine Veterinary Science 35 3 (2015): 206-212. http://www.scopus.com/inward/record.url?eid=2-s2.0-84923346047&partnerID=MN8TOARS. 10.1016/j.jevs.2014.12.017 Silva, Carolina. "During cooled storage the extender influences processed autophagy marker light chain 3 (LC3B) of stallion spermatozoa". Animal Reproduction Science (2014): 10.1016/j.anireprosci.2014.01.001 Silva, Carolina. "Effect of Overnight Staining on the Quality of Flow Cytometric Sorted Stallion Sperm: Comparison with Traditional Protocols". Reproduction in Domestic Animals (2014): 10.1111/rda.12431 Gallardo Bolaños, J.M.; Balao Da Silva, C.M.; Martín Muñoz, P.; Morillo Rodríguez, A.; Plaza Dávila, M.; Rodríguez-Martínez, H.; Aparicio, I.M.; et al. "Phosphorylated AKT preserves stallion sperm viability and motility by inhibiting caspases 3 and 7". Reproduction 148 2 (2014): 221-235. http://www.scopus.com/inward/record.url?eid=2-s2.0-84903608415&partnerID=MN8TOARS. 10.1530/REP-13-0191 Gallardo Bolaños, J.M.; Balao da Silva, C.; Martín Muñoz, P.; Plaza Dávila, M.; Ezquerra, J.; Aparicio, I.M.; Tapia, J.; Ortega Ferrusola, C.; Peña, F.J.. "Caspase activation, hydrogen peroxide production and akt dephosphorylation occur during stallion sperm senescence". Reproduction in Domestic Animals 49 4 (2014): 657-664. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904042554&partnerID=MN8TOARS. 10.1111/rda.12343 Silva, Carolina. "Sperm Susceptibility to Oxidative Stress in the Retuertas Endangered Horse". Journal of Equine Veterinary Science (2013): 10.1016/j.jevs.2013.02.006 Balao da Silva, C.M.; Ortega Ferrusola, C.; Morillo Rodriguez, A.; Gallardo Bolaños, J.M.; Plaza Dávila, M.; Morrell, J.M.; Rodriguez Martínez, H.; et al. "Sex sorting increases the permeability of the membrane of stallion spermatozoa". Animal Reproduction Science 138 3-4 (2013): 241-251. http://www.scopus.com/inward/record.url?eid=2-s2.0-84876837492&partnerID=MN8TOARS. 10.1016/j.anireprosci.2013.02.021 Balao da Silva, C.M.; Spinaci, M.; Bucci, D.; Giaretta, E.; Peña, F.J.; Mari, G.; Galeati, G.. "Effect of sex sorting on stallion spermatozoa: Heterologous oocyte binding, tyrosine phosphorylation and acrosome reaction assay". Animal Reproduction Science 141 1-2 (2013): 68-74. http://www.scopus.com/inward/record.url?eid=2-s2.0-84883050617&partnerID=MN8TOARS. 10.1016/j.anireprosci.2013.07.008 Silva, Carolina. "The Mitochondria of Stallion Spermatozoa Are More Sensitive Than the Plasmalemma to Osmotic Induced Stress: Role of c-Jun N-terminal Kinase (JNKs) Pathway". Journal of Andrology (2012): 10.2164/jandrol.110.011957 Macías García, B.; Ortega Ferrusola, C.; Aparicio, I.M.; Miró-Morán, A.; Morillo Rodriguez, A.; Gallardo Bolaños, J.M.; González Fernández, L.; et al. "Toxicity of glycerol for the stallion spermatozoa: Effects on membrane integrity and cytoskeleton, lipid peroxidation and mitochondrial membrane potential". Theriogenology 77 7 (2012): 1280-1289. http://www.scopus.com/inward/record.url?eid=2-s2.0-84858288783&partnerID=MN8TOARS. 10.1016/j.theriogenology.2011.10.033 Balao da Silva, C.; Macías-García, B.; Morillo Rodriguez, A.; Gallardo Bolaños, J.M.; Tapia, J.A.; Aparicio, I.M.; Morrell, J.M.; et al. "Effect of Hoechst 33342 on stallion spermatozoa incubated in KMT or Tyrodes modified INRA96". Animal Reproduction Science 131 3-4 (2012): 165-171. http://www.scopus.com/inward/record.url?eid=2-s2.0-84860440056&partnerID=MN8TOARS. 10.1016/j.anireprosci.2012.01.003 Morillo Rodriguez, A.; Balao da Silva, C.; Macías-García, B.; Gallardo Bolaños, J.M.; Tapia, J.A.; Aparicio, I.M.; Ortega-Ferrusola, C.; Peña, F.J.. "Dimethylformamide Improves the In vitro Characteristics of Thawed Stallion Spermatozoa Reducing Sublethal Damage". Reproduction in Domestic Animals 47 6 (2012): 995-1002. http://www.scopus.com/inward/record.url?eid=2-s2.0-84868698645&partnerID=MN8TOARS. 10.1111/j.1439-0531.2012.02005.x Bolaños, J.M.G.; Morán, Á.M.; da Silva, C.M.B.; Rodríguez, A.M.; Dávila, M.P.; Aparicio, I.M.; Tapia, J.A.; Ferrusola, C.O.; Peña, F.J.. "Autophagy and apoptosis have a role in the survival or death of stallion spermatozoa during conservation in refrigeration". PLoS ONE 7 1 (2012): http://www.scopus.com/inward/record.url?eid=2-s2.0-84856266647&partnerID=MN8TOARS. 10.1371/journal.pone.0030688 Balao Da Silva, C.M.; MacÍas-García, B.; Miró-Morán, A.; González-Fernández, L.; Morillo-Rodriguez, A.; Ortega-Ferrusola, C.; Gallardo-Bolaños, J.M.; et al. "Melatonin reduces lipid peroxidation and apoptotic-like changes in stallion spermatozoa". Journal of Pineal Research 51 2 (2011): 172-179. http://www.scopus.com/inward/record.url?eid=2-s2.0-80051665420&partnerID=MN8TOARS. 10.1111/j.1600-079X.2011.00873.x