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My scientific career started already during my undergraduate studies at the Biotechnical Faculty, University of Ljubljana (Slovenia), where I was involved in different research projects (Fajs et al., AJMR 2013 and Graduation thesis). I performed my PhD project in an industry research center, Novartis Vaccine and Diagnostics (today GSK Vaccines) in Siena, Italy. I obtained a 3-year Marie Curie ITN Fellowship (ClostNet project) and integrated in a prestigious PhD program, Novartis Academy program, headed by the world-leading expert in vaccinology Dr. Rino Rappuoli. My PhD work, supervised by Dr. Meera Unnikrishnan, was a pioneer work on biofilm formation by the anaerobic gut pathogen Clostridium difficile (Dapa et al., Journal of Bacteriology, 2013), which is highly cited and regarded in the field, having been highlighted in multiple platforms. Part of my PhD project was done at the University of Nottingham, under the supervision of Prof. Nigel P. Minton. A PhD between industry and an academic lab offered me a unique opportunity to perform research at the interface between academy and industry, which extensively contributed to my research management skills. After obtaining PhD, awarded by the University of Bologna, I changed research field and country for my first postdoc. In the lab of Dr. Ivan Matic, a world-leader in the field of stress responses, at INSERM (Paris, France), I designed and developed a research project on integration between the general stress response (RpoS response) and the DNA damage stress response (SOS response) in Escherichia coli (Dapa et al., Genetics. 2017). In a parallel project, I studied the integration between the SOS regulon and the stringent response to starvation in E. coli, which resulted in a manuscript currently in preparation (Dapa and Matic, in preparation). Furthermore, I made the initial observations that led to a ground-breaking publication (Fleurier et al., Nucleic Acids Research. 2022), which was selected by the reviewers to be featured as a 'Breakthrough' article. Additionally, I constructed genetic tools to dissect translation fidelity, which led to an additional publication (Woo et al., Science Advances. 2018). To complement my knowledge on genetics and bacterial physiology with experience on host interactions, evolutionary biology and biochemistry, I once again changed research field and country for my second postdoc, joining Dr. Karina Xavier lab at the Instituto Gulbenkian de Ciência, in Portugal. My work on the effects of dietary regimens on evolution of a key member of the gut microbiota has been recently published in the prestigious journal Cell Host & Microbe (Dapa et al., Cell Host Microbe. 2022), where it was featured as the article of the month, selected for the cover image and highlighted in a preview comment (Garud et al., Cell Host Microbe. 2022). In November 2022 I integrated in the Andalusian Center for Development Biology (CABD-UPO-CSIC) in Seville (Spain) with a María Zambrano International Talent Attraction Fellowship. In November 2023 I started as Junior PI (Beatriz Galindo Senior Distinguished Researcher Fellowship) at the same institute. I secured funds by obtaining a Spanish national project from "Proyectos de generación de conocimiento 2022, AEI" as a main and only PI, which allowed me to start a career as an independent researcher within the host institution. I was further awarded with a prestigious Ramon y Cajal contract, which I declined in favor of Beatriz Galindo Senior Fellowship. In sum, I worked in diverse research areas in the field of microbiology, in laboratories located in six different countries across Europe. I studied different subjects, from host-pathogen interaction to microbial genetics, stress responses, translation fidelity, and microbiota adaptation to nutritional perturbation. This wide-ranging experience allowed me to obtain knowledge on diverse biological systems and provided me with expertise in a many different research techniques.
Identification

Personal identification

Full name
Tanja Dapa

Citation names

  • Dapa, Tanja
  • Ðapa Tanja

Author identifiers

Ciência ID
521F-E8F5-F606
ORCID iD
0000-0002-5989-362X

Email addresses

  • tdap@upo.es (Professional)

Telephones

Mobile phone
  • (+34) 641753817 (Personal)

Addresses

  • Andalusian Center for Development Biology, Ctra. de Utrera, km. 1, 41013 , Sevilla, Montequinto, Spain (Professional)

Knowledge fields

  • Natural sciences - Biological Sciences - Microbiology
  • Natural sciences - Biological Sciences - Molecular Biology
  • Natural sciences - Biological Sciences - Evolutionary Biology
  • Medical and Health Sciences - Health Sciences - Infectious Diseases
  • Medical and Health Sciences - Basic Medicine - Immunology

Languages

Language Speaking Reading Writing Listening Peer-review
Slovenian (Mother tongue)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Italian Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Serbian Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Bosnian Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Croatian Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
Portuguese Upper intermediate (B2) Upper intermediate (B2) Intermediate (B1) Upper intermediate (B2)
Spanish; Castilian Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2) Upper intermediate (B2)
Education
Degree Classification
2014/04/11
Concluded
Ph.D. Program in Cellular and Molecular Biology (Dottorato di Ricerca)
Università di Bologna, Italy

GSK Vaccines SRL, Italy
"Biofilm formation by the anaerobic pathogen Clostridium difficile" (THESIS/DISSERTATION)
N/A
2010/06/09
Concluded
Interdepartmental programme in microbiology (Laurea)
Univerza v Ljubljani, Slovenia

Univerza v Ljubljani Biotehniska fakulteta, Slovenia
"Identification of unknown proteins, which regulate the SOS response of bacterium Escherichia coli" (THESIS/DISSERTATION)
10/10
Affiliation

Science

Category
Host institution
Employer
2023/11 - Current Principal Investigator (Research) Centro Andaluz de Biología del Desarrollo, Spain
Centro Andaluz de Biología del Desarrollo, Spain
(...)
2022/11 - 2023/10 Postdoc (Research) Centro Andaluz de Biología del Desarrollo, Spain
Centro Andaluz de Biología del Desarrollo, Spain
(...)
2017/06 - 2022/11 Postdoc (Research) Instituto Gulbenkian de Ciência, Portugal
2014/06/01 - 2016/04/30 Postdoc (Research) INSERM, France
2013/09/01 - 2013/11/05 Researcher (Research) University of Nottingham, United Kingdom
2010/09/01 - 2013/08/31 Research Trainee (Research) GSK Vaccines SRL, Italy
2011/05/15 - 2011/07/01 Research Trainee (Research) University of Nottingham, United Kingdom
2007/11/01 - 2010/06/09 Research Trainee (Research) Univerza v Ljubljani Biotehniska fakulteta, Slovenia
Projects

Grant

Designation Funders
2023/09/01 - 2026/08/31 Multiplicity of Polysaccharide Utilization Loci underlies synergistic coevolution between Bacteroides thetaiotaomicron and the mucosal immunity of the mammalian gut
PID2022-136800NA-I00
Principal investigator
Centro Andaluz de Biología del Desarrollo, Spain

Universidad Pablo de Olavide, Spain
Agencia Estatal de Investigación
Ongoing
2018/10 - 2022/02 Identificação de novos sinais de deteção de quorum na microbiota dos intestinos em mamíferos
Provided by PTCRIS: PTDC/BIA-MIC/30487/2017
Fundação para a Ciência e a Tecnologia
2010/09/01 - 2013/08/31 Biofilm formation by the anaerobic pathogen Clostridium difficile
PhD Student Fellow
GSK Vaccines SRL, Italy
European Commission
Concluded
2010/09/01 - 2013/08/31 Vaccine development against pathogen bacterium Clostridium difficile
/
PhD Student Fellow
GSK Vaccines SRL, Italy
Concluded

Contract

Designation Funders
2023/11 - Current Ayuda para contratos Beatriz Galindo Señor (2022)
BG22/00101
Researcher
Centro Andaluz de Biología del Desarrollo, Spain
Ministerio de Ciencia, Innovación y Universidades
Ongoing
2023 - Current Ayudas para contratos Ramón y Cajal (RYC) 2022
RYC2022-036163-I
Researcher
Agencia Estatal de Investigación
2017/06 - Current Bacterial adaptation to the mouse gut
/
Post-doc
Instituto Gulbenkian de Ciência, Portugal
Ongoing
2022/11 - 2023/10 Maria Zambrano are talent-attraction fellowship
/
Post-doc Fellow
Universidad Pablo de Olavide

Ministerio de Ciencia, Innovación y Universidades
Concluded
2014/06/01 - 2016/04/30 SOS response and overlapping stress responses in Escherichia coli
/
Post-doc
INSERM, France
Fonds AXA pour la Recherche
Concluded
2013/09 - 2013/11 Role of antisigma-factor RsbW in biofilm formation and sporulation of Clostridium difficile
/
Research Fellow
University of Nottingham, United Kingdom

Other

Designation Funders
2009/09 - 2010/06/09 Identification of unknown proteins, which regulate the SOS response of bacterium Escherichia coli
/
Master Student Fellow
Univerza v Ljubljani Biotehniska fakulteta, Slovenia
2007/11 - 2009/09 Genetic diversity of Escherichia coli isolated from healthy cattle
Other
Univerza v Ljubljani Biotehniska fakulteta, Slovenia
Outputs

Publications

Conference poster
  1. Dapa, Tanja; Ramiro, Ricardo S; Miguel F. Pedro; Gordo, Isabel; Karina B. Xavier. "Nutritional regimes leave genetic signature in keystone member of gut microbiota". Paper presented in World Microbe Forum, an ASM & FEMS Collaboration, 2021.
  2. Dapa, Tanja. "Diet Shapes Evolution of a Key Member of the Microbiota". Paper presented in INTERNATIONAL MEETING OF THE PORTUGUESE SOCIETY OF GENETICS, 2020.
  3. Dapa, Tanja. "Bacterial adaptation to diet-induced gut dysbiosis". Paper presented in Microbial eco-evolutionary dynamics. (IGC Symposium 2018, Oeiras Portugal), 2018.
  4. Dapa, Tanja. "SOS – Lost in translation". Paper presented in Molecular Genetics of Bacteria and Phages Meeting. (University of Wisconsin-Madison, USA)., 2015.
  5. Dapa, Tanja. "Clostridium difficile biofilm formation". Paper presented in EMBO/FEBS course 'Host-Microbes Interactions' (Spetses, Greece)., 2013.
  6. Dapa, Tanja. "Multiple Factors Modulate Biofilm Formation by the Anaerobic Pathogen Clostridium difficile". Paper presented in Clostnet II conference (Nottingham, UK)., 2013.
  7. Dapa, Tanja. "Multiple Factors Modulate Biofilm Formation by the Anaerobic Pathogen Clostridium difficile". Paper presented in 6th ASM Conference on Biofilms (Miami, USA)., 2012.
  8. Dapa, Tanja. "Identification and Characterization of Surface-Associated and Secreted Proteins of Clostridium difficile". Paper presented in ClostNet Open Conference I. University of Nottingham, UK., 2010.
  9. Dapa, Tanja. "Genetic Diversity of Escherichia coli Isolates from Feces of Healthy Cattle". Paper presented in Joint Congress of the Slovenian Biochemical Society and Slovenian Genetics Society. Otocec, Slovenija, 2009.
  10. Dapa, Tanja. "Genetic Diversity of Escherichia coli Isolates from Feces of Healthy Cattle". Paper presented in 4th Congress of the Slovenian Microbiological Society with International Participation, 2008.
Journal article
  1. Jeffrey K. J. Cheng; Tanja Ðapa; Ivan Y. L. Chan; Thomas MacCreath; Ross Slater; Meera Unnikrishnan. "The regulatory role of anti-sigma factor, RsbW, in Clostridioides difficile stress response, persistence and infection". Journal of Bacteriology (2023): https://journals.asm.org/doi/full/10.1128/jb.00466-22.
    10.1128/jb.00466-22
  2. Tanja Dapa; Daniel PGH Wong; Kimberly S Vasquez; Karina B Xavier; Kerwyn Casey Huang; Benjamin H Good. "Within-host evolution of the gut microbiome". Current Opinion in Microbiology 71 (2023): 102258-102258. http://dx.doi.org/10.1016/j.mib.2022.102258.
    10.1016/j.mib.2022.102258
  3. Sebastien Fleurier; Tanja Dapa; Olivier Tenaillon; Ciarán Condon; Ivan Matic. "rRNA operon multiplicity as a bacterial genome stability insurance policy". Nucleic Acids Research (2022): https://doi.org/10.1093/nar/gkac332.
    10.1093/nar/gkac332
  4. Dapa, Tanja; Ramiro, Ricardo Serotte; Pedro, Miguel Filipe; Gordo, Isabel; Xavier, Karina Bivar. "Diet leaves a genetic signature in a keystone member of the gut microbiota". Cell Host & Microbe 30 2 (2022): 183-199.e10. http://dx.doi.org/10.1016/j.chom.2022.01.002.
    10.1016/j.chom.2022.01.002
  5. Woo, Anthony C.; Faure, Louis; Dapa, Tanja; Matic, Ivan. "Heterogeneity of spontaneous DNA replication errors in single isogenic Escherichia coli cells". Science Advances 4 6 (2018): http://dx.doi.org/10.1126/sciadv.aat1608.
    10.1126/sciadv.aat1608
  6. Dapa, Tanja; Fleurier, Sébastien; Bredeche, Marie-Florence; Matic, Ivan. "The SOS and RpoS Regulons Contribute to Bacterial Cell Robustness to Genotoxic Stress by Synergistically Regulating DNA Polymerase Pol II". Genetics 206 3 (2017): 1349-1360. http://dx.doi.org/10.1534/genetics.116.199471.
    10.1534/genetics.116.199471
  7. Dapa, Tanja; Unnikrishnan, Meera. "Biofilm formation byClostridium difficile". Gut Microbes 4 5 (2013): 397-402. http://dx.doi.org/10.4161/gmic.25862.
    10.4161/gmic.25862
  8. Luka, FAJS; Mateja, JELEN; Maja, BORIC; Tanja, ÐAPA; Darja, ŽGUR BERTOK; Marjanca, STARCIC ERJAVEC. "The discriminative power in determining genetic diversity of Escherichia coli isolates: Comparing ERIC-PCR with AFLP". African Journal of Microbiology Research 7 20 (2013): 2416-2419. http://dx.doi.org/10.5897/ajmr12.1103.
    10.5897/ajmr12.1103
  9. Dapa, T.; Leuzzi, R.; Ng, Y.K.; Baban, S.T.; Adamo, R.; Kuehne, S.A.; Scarselli, M.; et al. "Multiple factors modulate biofilm formation by the anaerobic pathogen Clostridium difficile". Journal of Bacteriology 195 3 (2013): 545-555. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873037097&partnerID=MN8TOARS.
    10.1128/JB.01980-12
  10. Ðapa Tanja; Leuzzi, R.; Ng, Y. K.; Baban, S. T.; Adamo, R.; Kuehne, S. A.; Scarselli, M.; et al. "Multiple Factors Modulate Biofilm Formation by the Anaerobic Pathogen Clostridium difficile". Journal of Bacteriology 195 3 (2012): 545-555. http://dx.doi.org/10.1128/jb.01980-12.
    10.1128/jb.01980-12
Thesis / Dissertation
  1. Dapa, Tanja. "Biofilm formation by the anaerobic pathogen Clostridium difficile". PhD, Università di Bologna, 2014. http://amsdottorato.unibo.it/6343/1/DAPA_Tanja_Tesi.pdf.
  2. Dapa, Tanja. "Identification of unknown proteins, which regulate the SOS response of bacteria Escherichia coli". Degree, Univerza v Ljubljani Biotehniska fakulteta, 2010. http://www.digitalna-knjiznica.bf.uni-lj.si/dn_dapa_tanja.pdf.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2024/09/11 3rd Polish-Czech Probiotic Conference 3rd Polish-Czech Probiotic Conference
(Wroclaw, Poland)
2024/02/22 XV Workshop de la Sociedad Española de Microbiota Probióticos y Prebióticos The effect of diet on the genetic adaptation of the gut keystone species
SEMiPyP (Seville, Spain)
2023/03/11 11th edition of the Gut Microbiota for Health Summit Genetic adaptation of keystone species
GMFH (Prague, Czech Republic)
2023/02/23 Slovenian microbiological society virtual monthly seminar. Online. Genetic adaptation of a gut keystone member to the good, the bad & the ugly diet
2022/09/08 XIII Reunión del grupo de Microbiologia Molecular Tell me what you eat and I will tell you how your microbiota evolves
SEM (Granada, Spain)
2022/07/05 7th Annual Meeting of the International Society for Evolution, Medicine, and Public Health Tell me what you eat, and I tell you how your bacteria evolve
(Lisbon, Portugal)
2022/05/04 Effects of the good, the bad & the ugly diets on the genetic signature of your gut bacteria Seminar
Ibimed (Aveiro, Portugal)
2022/03/28 The impact of diet on the evolution of a key member of the gut microbiota SymbNET Interim Meeting
SymbNET - Genomics and Metabolomics in a Host-Microbe Symbiosis Network Project (Oeiras, Portugal)
2021/12/17 Effects of diet on the evolution of a key member of the gut microbiota Institutional seminar
Andalusian Centre for Developmental Biology (CABD) (Seville, Spain)
2019/10/09 Diet Shapes Evolution of a Key Member of the Microbiota Illumina user meeting, 6th Reuniao UGM
Instituto Gulbenkian de Ciencia (Oeiras, Portugal)
2017/05/06 Life and science after ClostNet Clospore Midterm review meeting
(Lisbon, Portugal)
2013/09/06 Clostridium difficile biofilm formation EMBO/FEBS course 'Host-Microbes Interactions'
EMBO and FEBS (Spetses , Greece)
2013/03/18 Multiple Factors Modulate Biofilm Formation by the Anaerobic Pathogen Clostridium difficile Clostnet II conference
University of Nottingham (Nottingham, United Kingdom)

Supervision

Thesis Title
Role
Degree Subject (Type)
Institution / Organization
2019/09/01 - Current Identification of quorum sensing regulated traits in Bacteroides thetaiotaomicron, the most prevalent member of the human gut microbiota
Co-supervisor
Microbiology (PhD)
Instituto Gulbenkian de Ciência, Portugal
2023/01 - 2023/06 Análisis de los determinantes de la eficiencia del ARN guía de Cas12 en bacterias
Co-supervisor
Universidad Pablo de Olavide Departamento de Biología Molecular e Ingeniería Bioquímica, Spain
2023/01 - 2023/06 Caracterización de genes relacionados con la formación de biofilm en Pseudomonas syringae pv. tomato DC3000
Co-supervisor
Universidad Pablo de Olavide Departamento de Biología Molecular e Ingeniería Bioquímica, Spain
2023/01 - 2023/06 EVALUACIÓN DEL SISTEMA DE REPARACIÓN NON-HOMOLOGOUS END JOINING DE Sphingopyxis granuli TFA EN Pseudomonas putida
Co-supervisor
Universidad Pablo de Olavide Departamento de Biología Molecular e Ingeniería Bioquímica, Spain
2019/09/01 - 2022/11 Identification of quorum sensing regulated mechanisms that play role in microbiota colonization of the mouse gut
Co-supervisor
Microbiology (Master)
Instituto Gulbenkian de Ciência, Portugal

Event organisation

Event name
Type of event (Role)
Institution / Organization
2017/10/15 - 2018/12/31 MICRO ECO-EVO DYNAMICS. This meeting aims to discuss recent developments in microbial eco-evolutionary dynamics research and serve as a venue for integrating the results obtained from different fields of evolution and ecology. By bringing together experts on different aspects of microbial evolution and ecology (e.g. experimental evolution, comparative genomics, ecological interactions, and theoretical biology), we hope to facilitate the development of new research strategies to better understand the generation and maintenance of microbial diversity. (2018/10/22 - 2018/10/24)
Symposium (Co-organisor)
Instituto Gulbenkian de Ciência, Portugal
2007 - 2007 LIFE WITH SCIENCE. International Life Science Students’ Conference (~100 participants). University of Ljubljana, Slovenia. Help with the organization. (2007/11/07 - 2007/11/11)
Conference (Co-organisor)
Univerza v Ljubljani Biotehniska fakulteta, Slovenia

Event participation

Activity description
Type of event
Event name
Institution / Organization
2019/12/16 - 2019/12/17 CONGENTO Course for Upgrade to Function B - animal handling, Design of Procedures and Projects
Workshop
CONGENTO Course for Upgrade to Function B
Fundação Champalimaud, Portugal
2017/10/16 - 2017/10/19 Course for animal handling
Workshop
Course for animal handling. Sociedade Portuguesa de Ciências em Animais de Laboratório (SPCAL), developed along guidelines established by the Federation of European Laboratory Animal Science Associations (FELASA).
2013/03/13 - 2013/03/17 Workshop on Clostridial Gene Technologies - part II
Workshop
Workshop on Clostridial Gene Technologies
University of Nottingham, United Kingdom
2012/09/29 - 2012/09/29 Workshop on Animal Models of Biofilm Infections, during the 6th ASM Conference on Biofilms. Miami, USA
Workshop
Workshop on Animal Models of Biofilm Infections
American Society for Microbiology, United States
2011/03/17 - 2011/03/20 Workshop on transcriptomics - microarray and RNA extraction in Clostridium.
Workshop
Transcriptomics Workshop at the Department of Microbiology (TUM)
Technische Universität München, Germany
2010/12/11 - 2010/12/15 Genetics tools for Clostridium, part I.
Workshop
Workshop on Gene Technologies I
University of Nottingham, United Kingdom

Association member

Society Organization name Role
2022 - Current Member of Sociedad Española de Microbiología (SEM)
2022 - Current Member of Sociedad Española de Bioquímica y Biología Molecular (SEBBM)
2021 - Current Member of Slovenian Microbiological Society (SMD)
2012 - Current Member of American Society for Microbiology (ASM)
2006 - 2010 Member of Students Microbiological Society Slovenia (DŠMS)

Committee member

Activity description
Role
Institution / Organization
2006 - 2010 President of Student Council, Biotechnical Faculty, Ljubljana, Slovenia. Member of the Faculty Committees as a student representative, Biotechnical Faculty, University of Ljubljana, Slovenia. Member of Student Council of Biotechnical Faculty and of Student Council of University of Ljubljana, Slovenia.
President / Vice-president
Univerza v Ljubljani Biotehniska fakulteta, Slovenia

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2022/05 - Current Career Development for Young Scientists. HFP Consulting (Outros)
2022/01 - 2022/01 Virtual EMBL Conference: The next generation in infection biology. Online.
2021 - 2022 Lecturer - IBB PhD program. Host-Microbe Interactions Course (Curso de doutoramento (conclusão de unidades curriculares)) Instituto Gulbenkian de Ciência, Portugal

Interview (newspaper / magazine)

Activity description Newspaper / Forum
2022/01/26 Disseminated of scientific results to the general public through the media RTP Madeira

Interview (tv / radio show)

Program Topic
2017/08/17 - Current Meta PHoDcast. Meta PHoDcast are conversation with young researchers about life, space and everything. The hosts and the guests are scientists from various fields of science. Most of our guest are doctoral students before completing their doctorate, and occasionally, Slovenian postdoctoral researchers from abroad. Young Slovenian scientists interviewed about science, life, and everything.

Journal scientific committee

Journal title (ISSN) Publisher
2013 - Current BMC Microbiology (1471-2180) Springer (Biomed Central Ltd.)

Other jury / evaluation

Activity description Institution / Organization
2013 - Current Reviewer for BMC Microbiology, Scientific reports, iScience, Cell Reports, co-reviewing for Cell Host & Microbe.
Distinctions

Award

2017 Seal of Excellence
European Commission, Belgium

Other distinction

2010 Marie Curie initial training fellowship - ClostNet