Rui Appelberg Gaio Lima (531D-7CB1-D366)

Curriculum Vitae

Rui Appelberg Gaio Lima

Rui Appelberg got his M.D. in 1984, his PhD in 1992, and his "Agregação" in 2002 at the Instituto de Ciências Biomédicas de Abel Salazar (ICBAS) of the University of Porto where he is Full Professor since 2003. He has been involved in the teaching of Microbiology, Immunology and Infection Biology. His research dealt with the study of Microbiology and Immunology of Infection at the IBMC where he was Group Leader until 2015. He now belongs to the staff of the Vilanova lab at the I3S. Specifically he studied the immune response, vaccines and the development of immune pathology to mycobacterial infections (Mycobacterium avium and M. tuberculosis). He published over one hundred papers in international journals indexed by PubMed. He was the PhD advisor of 12 students and co-advisor of several others. He is presently the PhD co-supervisor of one student at the University of Minho and another from the University of Porto. He won several scientific prizes including the First Pfizer Award for three times. He acted as Associate Editor of The Journal of Immunology and belongs to the Editorial Board of Infection and Immunity. Past collaborations included researchers from France, Spain, UK, USA, Denmark, The Netherlands, Germany and Norway.

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Identification

Personal identification

Full name: Rui Appelberg Gaio Lima

Citation names

Appelberg, Rui

Author identifiers

Ciência ID: 531D-7CB1-D366
ORCID iD: 0000-0002-9447-0665

Websites

https://sigarra.up.pt/icbas/pt/func_geral.formview?p_codigo=411832 (Scholar)

Education

	Degree	Classification
2002/07 Concluded	Agregação (Título de Agregado) Major in Microbiologia Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal	Aprovado
1992/12 Concluded	doutoramento (Doutoramento) Major in Microbiologia Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal	Aprovado
1978/10 - 1984/07 Concluded	Medicina (Licenciatura) Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal	16

Affiliation

Teaching in Higher Education

	Category Host institution	Employer
2003/11 - Current	Full Professor (University Teacher)	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

1997 - 2003	Associate Professor (University Teacher)	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

1993 - 1997	Assistant Professor (University Teacher)	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Projects

Contract

	Designation	Funders
2018/07/15 - 2021/07/14	Bases imunológicas da suscetibilidade às infeções por Mycobacterium tuberculosis PTDC/SAU-INF/28463/2017 Researcher Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Ongoing
2018/07/01 - 2021/06/30	Regeneração Tímica: Desenvolvimento de um programa de descoberta em larga escala para identificar novos compostos bioativos e determinantes moleculares que induzem a expressão de Foxn1 nas células epiteliais tímicas. PTDC/MED-IMU/29129/2017 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Ongoing
2018/06/01 - 2021/05/31	Desenvolvimento de uma vacina experimental contra a neosporose em bovinos PTDC/CVT-CVT/31020/2017 Researcher Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Ongoing
2010 - 2014	A New Approach to Fight Tuberculosis HMSP-ICT/0024/2010	N/A
2010/04/01 - 2013/03/31	O granuloma micobacteriano PTDC/SAU-MII/099102/2008 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Concluded
2007 - 2010	A new approach for developing a less Immunosuppressive Vaccine for TuBerculosis (ImmunoVacTB) LSHP-CT-2007-037388 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Concluded
2005/01/01 - 2007/12/31	Micobactérias e metabolismo do azoto POCTI/BIA-BCM/57169/2004 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Concluded
2003/12/01 - 2005/11/30	Depleção linfocitária T durante micobacterioses crónicas: implicações para doentes com SIDA PSIDA/MGI/49647/2003	N/A Concluded
2001/01/15 - 2004/01/14	IMUNIDADE A MYCOBACTERIUM AVIUM: MELHORAMENTO DA ACTIVIDADE ANTI-MICOBACTERIANA DO MACRÓFAGO POCTI/MGI/32629/2000 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1999 - 2004	Interfering with mycobacteria specific biosynthetic pathways: a rational approach to antimycobacterial antibiotics COST Action D13/0002/99 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Concluded
1998/06/01 - 2002/05/31	European Network for Investigation of the Pathogenesis of Psoriasis vulgaris ERBFMRXCT 980205 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Concluded
1998 - 2001	O ferro nas infecções por Mycobacterium avium e outras micobactérias PRAXIS/C/SAU/13232/1998 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Concluded
1997/11/01 - 2000/10/31	Human antibody repertoires in transgenic mice: generation, developmental regulation and therapeutic aplication of high affinity human antibodies Biotech BIO4972284 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Concluded
1997 - 2000	Development of a tuberculosis vaccine with consistent efficacy in different regions of the world INCO-DC: ERB-IC 18CT970254 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Concluded
1997 - 2000	HIV and HIV-related opportunistic infections. Study of iron chelation EC Biomed: BMH4-CT97-2387 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
1996 - 1999	Biologia da infecção por Mycobacterium avium PRAXIS XXI: PSAU/P/SAU/58/96 Principal investigator Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1996 - 1999	Biologia da infecção por Mycobacterium avium PRAXIS XXI: PSAU/P/SAU/58/96	N/A
1995 - 1998	Cooperação entre Neutrófilos e Macrófagos na Defesa do Hospedeiro Conta a Infecção PECS/C/SAU/60/95 Researcher Universidade do Porto, Portugal
1994 - 1998	Development of a new vaccine against tuberculosis STD program: TS3*CT94-0313 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Concluded
1994 - 1997	Secreção de Proteínas em Mycobacterium Avium PRAXIS/2/2.1/BIA/247/94 Principal investigator Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1992 - 1995	Imunidade nas infecções por micobactérias. Modelos de infecção do ratinho por Mycobacterium avium JNICT: STRDA/C/SAU/346/92 Principal investigator Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal	Concluded

Outputs

Publications

Book

Sarmento, A.; Campos, N.; Appelberg, R.; Magro, F.. Defective macrophage function in crohn's disease. 2012.

Journal article

Mariana Resende; Catarina M. Ferreira; Ana Margarida Barbosa; Marcos S. Cardoso; Jeremy Sousa; Margarida Saraiva; António G. Castro; Rui Appelberg; Egídio Torrado. "Myeloid HIF-1a regulates pulmonary inflammation during experimental Mycobacterium tuberculosis infection". Immunology (2020): https://doi.org/10.1111/imm.13131.
10.1111/imm.13131
Ana Cordeiro Gomes; Ana C. Moreira; Tânia Silva; João V. Neves; Gonçalo Mesquita; Agostinho A. Almeida; Palmira Barreira-Silva; et al. "IFN-¿–Dependent Reduction of Erythrocyte Life Span Leads to Anemia during Mycobacterial Infection". The Journal of Immunology (2019): ji1900382-ji1900382. https://doi.org/10.4049/jimmunol.1900382.
10.4049/jimmunol.1900382
Mariana Resende; Marcos S. Cardoso; Ricardo Fróis-Martins; Margarida Borges; Michael B. Jordan; António Gil Castro; Rui Appelberg. "TNF-Mediated Compensatory Immunity to Mycobacterium avium in the Absence of Macrophage Activation by IFN-¿". The Journal of Immunology (2019): ji1801594-ji1801594. https://doi.org/10.4049/jimmunol.1801594.
10.4049/jimmunol.1801594
Resende, M.; Cardoso, M.S.; Ribeiro, A.R.; Flórido, M.; Borges, M.; Gil Castro, A.; Alves, N.L.; Cooper, A.M.; Appelberg, R.. "Innate IFN-¿-Producing cells developing in the absence of IL-2 receptor common ¿-chain". Journal of Immunology 199 4 (2017): 1429-1439. http://www.scopus.com/inward/record.url?eid=2-s2.0-85027298608&partnerID=MN8TOARS.
10.4049/jimmunol.1601701
Moreira-Teixeira, L.; Sousa, J.; McNab, F.W.; Torrado, E.; Cardoso, F.; Machado, H.; Castro, F.; et al. "Type i IFN inhibits alternative macrophage activation during mycobacterium tuberculosis infection and leads to enhanced protection in the absence of IFN-¿ signaling". Journal of Immunology 197 12 (2016): 4714-4726. http://www.scopus.com/inward/record.url?eid=2-s2.0-85002328285&partnerID=MN8TOARS.
10.4049/jimmunol.1600584
Silva, J.P.; Appelberg, R.; Gama, F.M.. "Antimicrobial peptides as novel anti-tuberculosis therapeutics". Biotechnology Advances (2016): http://www.scopus.com/inward/record.url?eid=2-s2.0-84970023327&partnerID=MN8TOARS.
10.1016/j.biotechadv.2016.05.007
Silva, J.P.; Gonçalves, C.; Costa, C.; Sousa, J.; Silva-Gomes, R.; Castro, A.G.; Pedrosa, J.; Appelberg, R.; Gama, F.M.. "Delivery of LLKKK18 loaded into self-assembling hyaluronic acid nanogel for tuberculosis treatment". Journal of Controlled Release 235 (2016): 112-124. http://www.scopus.com/inward/record.url?eid=2-s2.0-84973165158&partnerID=MN8TOARS.
10.1016/j.jconrel.2016.05.064
Nazareth, N.; Magro, F.; Appelberg, R.; Silva, J.; Gracio, D.; Coelho, R.; Cabral, J.M.; et al. "Increased viability but decreased culturability of Mycobacterium avium subsp. paratuberculosis in macrophages from inflammatory bowel disease patients under Infliximab treatment". Medical Microbiology and Immunology 204 6 (2015): 647-656. http://www.scopus.com/inward/record.url?eid=2-s2.0-84942197352&partnerID=MN8TOARS.
10.1007/s00430-015-0393-2
Cruz, A.; Torrado, E.; Carmona, J.; Fraga, A.G.; Costa, P.; Rodrigues, F.; Appelberg, R.; et al. "BCG vaccination-induced long-lasting control of Mycobacterium tuberculosis correlates with the accumulation of a novel population of CD4+IL-17+TNF+IL-2+ T cells". Vaccine 33 1 (2015): 85-91. http://www.scopus.com/inward/record.url?eid=2-s2.0-84919360521&partnerID=MN8TOARS.
10.1016/j.vaccine.2014.11.013
Cruz, A.; Ludovico, P.; Torrado, E.; Gama, J.B.; Sousa, J.; Gaifem, J.; Appelberg, R.; et al. "IL-17A promotes intracellular growth of Mycobacterium by inhibiting apoptosis of infected macrophages". Frontiers in Immunology 6 SEP (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84946550863&partnerID=MN8TOARS.
10.3389/fimmu.2015.00498
Nazareth, N.; Magro, F.; Machado, E.; Ribeiro, T.G.; Martinho, A.; Rodrigues, P.; Alves, R.; et al. "Prevalence of Mycobacterium avium subsp. paratuberculosis and Escherichia coli in blood samples from patients with inflammatory bowel disease". Medical Microbiology and Immunology 204 6 (2015): 681-692. http://www.scopus.com/inward/record.url?eid=2-s2.0-84942193176&partnerID=MN8TOARS.
10.1007/s00430-015-0420-3
Correia, A.; Ferreirinha, P.; Botelho, S.; Belinha, A.; Leitão, C.; Caramalho, I.; Teixeira, L.; et al. "Predominant role of interferon-¿ 3 in the host protective effect of CD8 + T cells against Neospora caninum infection". Scientific Reports 5 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84943770641&partnerID=MN8TOARS.
10.1038/srep14913
Marques, F.; Vale-Costa, S.; Cruz, T.; Marques, J.M.; Silva, T.; Neves, J.V.; Cortes, S.; et al. "Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection". Parasites and Vectors 8 1 (2015): http://www.scopus.com/inward/record.url?eid=2-s2.0-84950313576&partnerID=MN8TOARS.
10.1186/s13071-015-1259-6
Appelberg, R.; Moreira, D.; Barreira-Silva, P.; Borges, M.; Silva, L.; Dinis-Oliveira, R.J.; Resende, M.; et al. "The Warburg effect in mycobacterial granulomas is dependent on the recruitment and activation of macrophages by interferon-¿". Immunology 145 4 (2015): 498-507. http://www.scopus.com/inward/record.url?eid=2-s2.0-84936175105&partnerID=MN8TOARS.
10.1111/imm.12464
Cardoso, M.S.; Silva, T.M.; Resende, M.; Appelberg, R.; Borges, M.. "Lack of the transcription factor hypoxia-inducible factor 1a (HIF- 1a) in macrophages accelerates the necrosis of Mycobacterium avium-induced granulomas". Infection and Immunity 83 9 (2015): 3534-3544. http://www.scopus.com/inward/record.url?eid=2-s2.0-84939546765&partnerID=MN8TOARS.
10.1128/IAI.00144-15
Nazareth, N.; Magro, F.; Silva, J.; Duro, M.; Gracio, D.; Coelho, R.; Appelberg, R.; Macedo, G.; Sarmento, A.. "Infliximab therapy increases the frequency of circulating CD16⁺ monocytes and modifies macrophage cytokine response to bacterial infection". Clinical and Experimental Immunology 177 3 (2014): 703-711. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904751087&partnerID=MN8TOARS.
10.1111/cei.12375
Silva-Gomes, S.; Bouton, C.; Silva, T.; Santambrogio, P.; Rodrigues, P.; Appelberg, R.; Gomes, M.S.. "Mycobacterium avium infection induces H-ferritin expression in mouse primary macrophages by activating Toll-like receptor 2". PLoS ONE 8 12 (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84891960497&partnerID=MN8TOARS.
10.1371/journal.pone.0082874
Silva-Gomes, S.; Vale-Costa, S.; Appelberg, R.; Gomes, M.S.. "Iron in intracellular infection: To provide or to deprive?". Frontiers in Cellular and Infection Microbiology 3 DEC (2013): http://www.scopus.com/inward/record.url?eid=2-s2.0-84897926567&partnerID=MN8TOARS.
10.3389/fcimb.2013.00096
Vale-Costa, Silvia; Gomes-Pereira, Sandra; Teixeira, Carlos Miguel; Rosa, Gustavo; Rodrigues, Pedro Nuno; Tomas, Ana; Appelberg, Rui; Gomes, Maria Salome. "Iron Overload Favors the Elimination of Leishmania infantum from Mouse Tissues through Interaction with Reactive Oxygen and Nitrogen Species". Plos Neglected Tropical Diseases 7 2 (2013):
10.1371/journal.pntd.0002061
Silva-Gomes, S.; Appelberg, R.; Larsen, R.; Soares, M.P.; Gomes, M.S.. "Heme catabolism by heme oxygenase-1 confers host resistance to Mycobacterium infection". Infection and Immunity 81 7 (2013): 2536-2545. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879426573&partnerID=MN8TOARS.
10.1128/IAI.00251-13
Afonso-Barroso, A.; Clark, S.O.; Williams, A.; Rosa, G.T.; Nóbrega, C.; Silva-Gomes, S.; Vale-Costa, S.; et al. "Lipoarabinomannan mannose caps do not affect mycobacterial virulence or the induction of protective immunity in experimental animal models of infection and have minimal impact on in vitro inflammatory responses". Cellular Microbiology 15 4 (2013): 660-674. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874977321&partnerID=MN8TOARS.
10.1111/cmi.12065
Pearl, J.E.; Torrado, E.; Tighe, M.; Fountain, J.J.; Solache, A.; Strutt, T.; Swain, S.; Appelberg, R.; Cooper, A.M.. "Nitric oxide inhibits the accumulation of CD4⁺CD44^hiTbet⁺CD69^loT cells in mycobacterial infection". European Journal of Immunology 42 12 (2012): 3267-3279. http://www.scopus.com/inward/record.url?eid=2-s2.0-84871273202&partnerID=MN8TOARS.
10.1002/eji.201142158
Borges, M.; Barreira-Silva, P.; Flórido, M.; Jordan, M.B.; Correia-Neves, M.; Appelberg, R.. "Molecular and cellular mechanisms of Mycobacterium avium-induced thymic atrophy". Journal of Immunology 189 7 (2012): 3600-3608. http://www.scopus.com/inward/record.url?eid=2-s2.0-84866527721&partnerID=MN8TOARS.
10.4049/jimmunol.1201525
Campos, N.; Magro, F.; Castro, A.R.; Cabral, J.; Rodrigues, P.; Silva, R.; Appelberg, R.; et al. "Macrophages from IBD patients exhibit defective tumour necrosis factor-a secretion but otherwise normal or augmented pro-inflammatory responses to infection". Immunobiology 216 8 (2011): 961-970. http://www.scopus.com/inward/record.url?eid=2-s2.0-79959492512&partnerID=MN8TOARS.
10.1016/j.imbio.2011.01.002
Castro, H.; Teixeira, F.; Romao, S.; Santos, M.; Cruz, T.; Flórido, M.; Appelberg, R.; et al. "Leishmania mitochondrial peroxiredoxin plays a crucial peroxidase-unrelated role during infection: Insight into its novel chaperone activity". PLoS Pathogens 7 10 (2011): http://www.scopus.com/inward/record.url?eid=2-s2.0-80055096383&partnerID=MN8TOARS.
10.1371/journal.ppat.1002325
Rodrigues, Pedro N.; Gomes, Sandro S.; Neves, Joao V.; Gomes-Pereira, Sandra; Correia-Neves, Margarida; Nunes-Alves, Claudio; Stolte, Jens; et al. "Mycobacteria-induced anaemia revisited: A molecular approach reveals the involvement of NRAMP1 and lipocalin-2, but not of hepcidin". Immunobiology 216 10 (2011): 1127-1134.
10.1016/j.imbio.2011.04.004
Borges, M.; Rosa, G. T.; Appelberg, R.. "The death-promoting molecule tumour necrosis factor-related apoptosis inducing ligand (TRAIL) is not required for the development of peripheral lymphopenia or granuloma necrosis during infection with virulent Mycobacterium avium". Clinical and Experimental Immunology 164 3 (2011): 407-416.
10.1111/j.1365-2249.2011.04385.x
Carvalho, N.B.; Oliveira, F.S.; Durães, F.V.; de Almeida, L.A.; Flórido, M.; Prata, L.O.; Caliari, M.V.; Appelberg, R.; Oliveira, S.C.. "Toll-like receptor 9 is required for full host resistance to Mycobacterium avium infection but plays no role in induction of Th1 responses". Infection and Immunity 79 4 (2011): 1638-1646. http://www.scopus.com/inward/record.url?eid=2-s2.0-79953323282&partnerID=MN8TOARS.
10.1128/IAI.01030-10
Sonawane, A.; Santos, J.C.; Mishra, B.B.; Jena, P.; Progida, C.; Sorensen, O.E.; Gallo, R.; Appelberg, R.; Griffiths, G.. "Cathelicidin is involved in the intracellular killing of mycobacteria in macrophages". Cellular Microbiology 13 10 (2011): 1601-1617. http://www.scopus.com/inward/record.url?eid=2-s2.0-80052794411&partnerID=MN8TOARS.
10.1111/j.1462-5822.2011.01644.x
Fernandes, S.S.; Nunes, A.; Gomes, A.R.; de Castro, B.; Hider, R.C.; Rangel, M.; Appelberg, R.; Gomes, M.S.. "Identification of a new hexadentate iron chelator capable of restricting the intramacrophagic growth of Mycobacterium avium". Microbes and Infection 12 4 (2010): 287-294. http://www.scopus.com/inward/record.url?eid=2-s2.0-77649274461&partnerID=MN8TOARS.
10.1016/j.micinf.2010.01.003
Nobrega, C.; Roque, S.; Nunes-Alves, C.; Coelho, A.; Medeiros, I.; Castro, A.G.; Appelberg, R.; Correia-Neves, M.. "Dissemination of mycobacteria to the thymus renders newly generated T cells tolerant to the invading pathogen". Journal of Immunology 184 1 (2010): 351-358. http://www.scopus.com/inward/record.url?eid=2-s2.0-73949157248&partnerID=MN8TOARS.
10.4049/jimmunol.0902152
Flórido, M.; Borges, M.; Rodrigues, P.; Vale-Costa, S.; Salomé Gomes, M.; Appelberg, R.. "Constitutive expression of Bcl-2 in the haematopoietic compartment alters the metabolism of iron and increases resistance to mycobacterial infection". Clinical and Experimental Immunology 156 1 (2009): 61-68. http://www.scopus.com/inward/record.url?eid=2-s2.0-61849113352&partnerID=MN8TOARS.
10.1111/j.1365-2249.2008.03867.x
Florido, Manuela; McColl, Shaun R.; Appelberg, Rui. "Delayed recruitment of lymphocytes into the lungs of CD30-deficient mice during aerogenic Mycobacterium avium infections". Immunobiology 214 8 (2009): 643-652.
10.1016/j.imbio.2008.12.002
Gomes-Pereira, S.; Rodrigues, P.N.; Appelberg, R.; Gomes, M.S.. "Increased susceptibility to Mycobacterium avium in hemochromatosis protein HFE-deficient mice". Infection and Immunity 76 10 (2008): 4713-4719. http://www.scopus.com/inward/record.url?eid=2-s2.0-53649106524&partnerID=MN8TOARS.
10.1128/IAI.00612-08
Gomes, M. Salome; Fernandes, Sofia Sousa; Cordeiro, Joao V.; Gomes, Sandro Silva; Vieira, Andre; Appelberg, Rui. "Engagement of Toll-like receptor 2 in mouse macrophages infected with Mycobacterium avium induces non-oxidative and TNF-independent anti-mycobacterial activity". European Journal of Immunology 38 8 (2008): 2180-2189.
10.1002/eji.200737954
Lousada, S.; Flórido, M.; Appelberg, R.. "Virulence of Mycobacterium avium in mice does not correlate with resistance to nitric oxide". Microbial Pathogenesis 43 5-6 (2007): 243-248. http://www.scopus.com/inward/record.url?eid=2-s2.0-34548655034&partnerID=MN8TOARS.
10.1016/j.micpath.2007.06.003
Boelaert, J.R.; Vandecasteele, S.J.; Appelberg, R.; Gordeuk, V.R.. "The effect of the host's iron status on tuberculosis". Journal of Infectious Diseases 195 12 (2007): 1745-1753. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249863579&partnerID=MN8TOARS.
10.1086/518040
Nobrega, Claudia; Cardona, Pere-Joan; Roque, Susana; do O, Perpetua Pinto; Appelberg, Rui; Correia-Neves, Margarida. "The thymus as a target for mycobacterial infections". Microbes and Infection 9 14-15 (2007): 1521-1529.
10.1016/j.micinf.2007.08.006
Gobec, S.; Plantan, I.; Mravljak, J.; Švajger, U.; Wilson, R.A.; Besra, G.S.; Soares, S.L.; Appelberg, R.; Kikelj, D.. "Design, synthesis, biochemical evaluation and antimycobacterial action of phosphonate inhibitors of antigen 85C, a crucial enzyme involved in biosynthesis of the mycobacterial cell wall". European Journal of Medicinal Chemistry 42 1 (2007): 54-63. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846690975&partnerID=MN8TOARS.
10.1016/j.ejmech.2006.08.007
Appelberg, R.. "Neutrophils and intracellular pathogens: beyond phagocytosis and killing". Trends in Microbiology 15 2 (2007): 87-92. http://www.scopus.com/inward/record.url?eid=2-s2.0-33846694050&partnerID=MN8TOARS.
10.1016/j.tim.2006.11.009
Roque, S.; Nobrega, C.; Appelberg, R.; Correia-Neves, M.. "IL-10 underlies distinct susceptibility of BALB/c and C57BL/6 mice to Mycobacterium avium infection and influences efficacy of antibiotic therapy". Journal of Immunology 178 12 (2007): 8028-8035. http://www.scopus.com/inward/record.url?eid=2-s2.0-34250173749&partnerID=MN8TOARS.
Flórido, M.; Appelberg, R.. "Characterization of the deregulated immune activation occurring at late stages of mycobacterial infection in TNF-deficient mice". Journal of Immunology 179 11 (2007): 7702-7708. http://www.scopus.com/inward/record.url?eid=2-s2.0-38849194103&partnerID=MN8TOARS.
Appelberg, R.. "Pathogenesis of Mycobacterium avium infection: Typical responses to an atypical Mycobacterium?". Immunologic Research 35 3 (2006): 179-190. http://www.scopus.com/inward/record.url?eid=2-s2.0-33845894453&partnerID=MN8TOARS.
10.1385/IR:35:3:179
Flórido, M.; Appelberg, R.. "Genetic control of immune-mediated necrosis of Mycobacterium avium granulomas". Immunology 118 1 (2006): 122-130. http://www.scopus.com/inward/record.url?eid=2-s2.0-33645801985&partnerID=MN8TOARS.
10.1111/j.1365-2567.2006.02350.x
Lousada, Susana; Florido, Manuela; Appelberg, Rui. "Regulation of granuloma fibrosis by nitric oxide during Mycobacterium avium experimental infection". International Journal of Experimental Pathology 87 4 (2006): 307-315.
10.1111/j.1365-2613.2006.00487.x
Appelberg, R.. "Macrophage nutriprive antimicrobial mechanisms". Journal of Leukocyte Biology 79 6 (2006): 1117-1128. http://www.scopus.com/inward/record.url?eid=2-s2.0-33746881453&partnerID=MN8TOARS.
10.1189/jlb.0206079
Florido, M; Pearl, JE; Solache, A; Borges, M; Haynes, L; Cooper, AM; Appelberg, R. "Gamma interferon-induced T-cell loss in virulent Mycobacterium avium infection". Infection and Immunity 73 6 (2005): 3577-3586.
10.1128/IAI.73.6.3577-3586.2005
Florido, M; Goncalves, AS; Gomes, MS; Appelberg, R. "CD40 is required for the optimal induction of protective immunity to Mycobacterium avium". Immunology 111 3 (2004): 323-327.
10.1111/j.1365-2567.2004.01812.x
Flórido, M.; Borges, M.; Yagita, H.; Appelberg, R.. "Contribution of CD30/CD153 but not of CD27/CD70, CD134/OX40L, or CD137/4-1BBL to the optimal induction of protective immunity to Mycobacterium avium". Journal of Leukocyte Biology 76 5 (2004): 1039-1046. http://www.scopus.com/inward/record.url?eid=2-s2.0-7644226724&partnerID=MN8TOARS.
10.1189/jlb.1103572
Flórido, M.; Appelberg, R.. "Granuloma necrosis during Mycobacterium avium infection does not require tumor necrosis factor". Infection and Immunity 72 10 (2004): 6139-6141. http://www.scopus.com/inward/record.url?eid=2-s2.0-4644279510&partnerID=MN8TOARS.
10.1128/IAI.72.10.6139-6141.2004
Gomes, M.S.; Flórido, M.; Cordeiro, J.V.; Teixeira, C.M.; Takeuchi, O.; Akira, S.; Appelberg, R.. "Limited role of the Toll-like receptor-2 in resistance to Mycobacterium avium". Immunology 111 2 (2004): 179-185. http://www.scopus.com/inward/record.url?eid=2-s2.0-0842265556&partnerID=MN8TOARS.
10.1111/j.0019-2805.2003.01807.x
Pais, T.F.; Appelberg, R.. "Induction of Mycobacterium avium growth restriction and inhibition of phagosome-endosome interactions during macrophage during macrophage activation and apoptosis induction by picolinic acid plus IFN¿". Microbiology 150 5 (2004): 1507-1518. http://www.scopus.com/inward/record.url?eid=2-s2.0-2942627335&partnerID=MN8TOARS.
Flórido, M.; Correia-Neves, M.; Cooper, A.M.; Appelberg, R.. "The cytolytic activity of natural killer cells is not involved in the restriction of Mycobacterium avium growth". International Immunology 15 8 (2003): 895-901. http://www.scopus.com/inward/record.url?eid=2-s2.0-0041522521&partnerID=MN8TOARS.
10.1093/intimm/dxg089
Cooper, A.M.; Adams, L.B.; Dalton, D.K.; Appelberg, R.; Ehlers, S.. "IFN-¿ and NO in mycobacterial disease: New jobs for old hands". Trends in Microbiology 10 5 (2002): 221-226. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036568586&partnerID=MN8TOARS.
10.1016/S0966-842X(02)02344-2
Flórido, M.; Cooper, A.M.; Appelberg, R.. "Immunological basis of the development of necrotic lesions following Mycobacterium avium infection". Immunology 106 4 (2002): 590-601. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036040671&partnerID=MN8TOARS.
10.1046/j.1365-2567.2002.01459.x
Gonçalves, A.-S.; Appelberg, R.. "The involvement of the chemokine receptor CXCR2 in neutrophil recruitment in LPS-induced inflammation and in Mycobacterium avium infection". Scandinavian Journal of Immunology 55 6 (2002): 585-591. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036091025&partnerID=MN8TOARS.
10.1046/j.1365-3083.2002.01097.x
Brandt, L; Cunha, JF; Olsen, AW; Chilima, B; Hirsch, P; Appelberg, R; Andersen, P. "Failure of the Mycobacterium bovis BCG vaccine: Some species of environmental mycobacteria block multiplication of BCG and induction of protective immunity to tuberculosis". Infection and Immunity 70 2 (2002): 672-678.
10.1128/IAI.70.2.672-678.2002
Gomes, M.S.; Appelberg, R.. "NRAMP1- or cytokine-induced bacteriostasis of Mycobacterium avium by mouse macrophages is independent of the respiratory burst". Microbiology 148 10 (2002): 3155-3160. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036772316&partnerID=MN8TOARS.
Silva, R.A.; Flórido, M.; Appelberg, R.. "Interleukin-12 primes CD4+ T cells for interferon-¿ production and protective immunity during Mycobacterium avium infection". Immunology 103 3 (2001): 368-374. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034943449&partnerID=MN8TOARS.
10.1046/j.1365-2567.2001.01237.x
Silva, R.A.; Appelberg, R.. "Blocking the receptor for interleukin 10 protects mice from lethal listeriosis". Antimicrobial Agents and Chemotherapy 45 4 (2001): 1312-1314. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035080639&partnerID=MN8TOARS.
10.1128/AAC.45.4.1312-1314.2001
Leal, I.S.; Smedegård, B.; Andersen, P.; Appelberg, R.. "Failure to induce enhanced protection against tuberculosis by increasing T-cell-dependent interferon-¿ generation". Immunology 104 2 (2001): 157-161. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034771680&partnerID=MN8TOARS.
10.1046/j.1365-2567.2001.01305.x
Goncalves, AS; Appelberg, R. "Effects of recombinant granulocyte-colony stimulating factor administration during Mycobacterium avium infection in mice". Clinical and Experimental Immunology 124 2 (2001): 239-247.
10.1046/j.1365-2249.2001.01552.x
Boelaert, J.R.; Appelberg, R.; Gomes, M.S.; Blasi, E.; Mazzolla, R.; Grosset, J.; Lounis, N.; et al. "Experimental results on chloroquine and AIDS-related opportunistic infections [3]". Journal of Acquired Immune Deficiency Syndromes 26 3 (2001): 300-301. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035280585&partnerID=MN8TOARS.
Silva, R.A.; Pais, T.F.; Appelberg, R.. "Blocking the receptor for IL-10 improves antimycobacterial chemotherapy and vaccination". Journal of Immunology 167 3 (2001): 1535-1541. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035424768&partnerID=MN8TOARS.
Turner, J.; D'Souza, C.D.; Pearl, J.E.; Marietta, P.; Noel, M.; Frank, A.A.; Appelberg, R.; Orme, I.M.; Cooper, A.M.. "CD8- and CD95/95L-dependent mechanisms of resistance in mice with chronic pulmonary tuberculosis". American Journal of Respiratory Cell and Molecular Biology 24 2 (2001): 203-209. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035125898&partnerID=MN8TOARS.
Gomes, MS; Boelaert, JR; Appelberg, R. "Role of iron in experimental Mycobacterium avium infection". Journal of Clinical Virology 20 3 (2001): 117-122.
10.1016/S1386-6532(00)00135-9
Leal, IS; Florido, M; Andersen, P; Appelberg, R. "Interleukin-6 regulates the phenotype of the immune response to a tuberculosis subunit vaccine". Immunology 103 3 (2001): 375-381.
10.1046/j.1365-2567.2001.01244.x
Silva, R.A.; Pais, T.F.; Appelberg, R.. "Effects of interleukin-12 in the long-term protection conferred by a Mycobacterium avium subunit vaccine". Scandinavian Journal of Immunology 52 6 (2000): 531-533. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034528830&partnerID=MN8TOARS.
10.1046/j.1365-3083.2000.00816.x
Pedrosa, J.; Saunders, B.M.; Appelberg, R.; Orme, I.M.; Silva, M.T.; Cooper, A.M.. "Neutrophils play a protective nonphagocytic role in systemic Mycobacterium tuberculosis infection of mice". Infection and Immunity 68 2 (2000): 577-583. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033958803&partnerID=MN8TOARS.
10.1128/IAI.68.2.577-583.2000
Pais, T.F.; Appelberg, R.. "Macrophage control of mycobacterial growth induced by picolinic acid is dependent on host cell apoptosis". Journal of Immunology 164 1 (2000): 389-397. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033966376&partnerID=MN8TOARS.
Appelberg, R.; Leal, I.S.; Pais, T.F.; Pedrosa, J.; Flórido, M.. "Differences in resistance of C57BL/6 and C57BL/10 mice to infection by Mycobacterium avium are independent of gamma interferon". Infection and Immunity 68 1 (2000): 19-23. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033979338&partnerID=MN8TOARS.
Gonçalves, A.S.; Appelberg, R.. "Modulation of neutrophil influx with cell adhesion molecule specific antibodies during nonspecific and immune mediated inflammatory reactions". Scandinavian Journal of Immunology 51 5 (2000): 485-490. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034014562&partnerID=MN8TOARS.
10.1046/j.1365-3083.2000.00720.x
Silva, RA; Gomes, MS; Appelberg, R. "Minor role played by type I tumour necrosis factor receptor in the control of Mycobacterium avium proliferation in infected mice". Immunology 99 2 (2000): 203-207.
10.1046/j.1365-2567.2000.00937.x
Appelberg, R.; Leal, I.S.. "Mutants of Listeria monocytogenes defective in in vitro invasion and cell-to-cell spreading still invade and proliferate in hepatocytes of neutropenic mice". Infection and Immunity 68 2 (2000): 912-914. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033967266&partnerID=MN8TOARS.
10.1128/IAI.68.2.912-914.2000
Pais, T.F.; Cunha, J.F.; Appelberg, R.. "Antigen specificity of T-cell response to Mycobacterium avium infection in mice". Infection and Immunity 68 8 (2000): 4805-4810. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033943811&partnerID=MN8TOARS.
10.1128/IAI.68.8.4805-4810.2000
Gomes, M.S.; Dom, G.; Pedrosa, J.; Boelaert, J.R.; Appelberg, R.. "Effects of iron deprivation on Mycobacterium avium growth". Tubercle and Lung Disease 79 5 (1999): 321-328. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032860952&partnerID=MN8TOARS.
10.1054/tuld.1999.0216
Gomes, M.S.; Flórido, M.; Pais, T.F.; Appelberg, R.. "Improved clearance of Mycobacterium avium upon disruption of the inducible nitric oxide synthase gene". Journal of Immunology 162 11 (1999): 6734-6739. http://www.scopus.com/inward/record.url?eid=2-s2.0-0345593709&partnerID=MN8TOARS.
Gomes, M.S.; Paul, S.; Moreira, A.L.; Appelberg, R.; Rabinovitch, M.; Kaplan, G.. "Survival of Mycobacterium avium and Mycobacterium tuberculosis in acidified vacuoles of murine macrophages". International Journal of Leprosy and Other Mycobacterial Diseases 67 4 SUPPL. (1999): http://www.scopus.com/inward/record.url?eid=2-s2.0-33748279474&partnerID=MN8TOARS.
Leal, I.S.; Smedegård, B.; Andersen, P.; Appelberg, R.. "Interleukin-6 and interleukin-12 participate in induction of a type 1 protective T-cell response during vaccination with a tuberculosis subunit vaccine". Infection and Immunity 67 11 (1999): 5747-5754. http://www.scopus.com/inward/record.url?eid=2-s2.0-0345718513&partnerID=MN8TOARS.
Flórido, M.; Gonçalves, A.S.; Silva, R.A.; Ehlers, S.; Cooper, A.M.; Appelberg, R.. "Resistance of virulent Mycobacterium avium to gamma interferon-mediated antimicrobial activity suggests additional signals for induction of mycobacteriostasis". Infection and Immunity 67 7 (1999): 3610-3618. http://www.scopus.com/inward/record.url?eid=2-s2.0-17344388183&partnerID=MN8TOARS.
Silva, R.A.; Pais, T.F.; Appelberg, R.. "Evaluation of IL-12 in immunotherapy and vaccine design in experimental mycobacterium avium infections". Journal of Immunology 161 10 (1998): 5578-5585. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032533852&partnerID=MN8TOARS.
Pais, T.F.; Silva, R.A.; Smedegaard, B.; Appelberg, R.; Andersen, P.. "Analysis of T cells recruited during delayed-type hypersensitivity to purified protein derivative (PPD) versus challenge with tuberculosis infection". Immunology 95 1 (1998): 69-75. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031657702&partnerID=MN8TOARS.
10.1046/j.1365-2567.1998.00561.x
Gomes, M.S.; Appelberg, R.. "Evidence for a link between iron metabolism and Nramp1 gene function in innate resistance against Mycobacterium avium". Immunology 95 2 (1998): 165-168. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031783138&partnerID=MN8TOARS.
10.1046/j.1365-2567.1998.00630.x
Cooper, A.M.; Appelberg, R.; Orme, I.M.. "Immunopathogenesis of Mycobacterium avium infection.". Frontiers in bioscience : a journal and virtual library 3 (1998): http://www.scopus.com/inward/record.url?eid=2-s2.0-0000939824&partnerID=MN8TOARS.
Florido, M.; Appelberg, R.; Orme, I.M.; Cooper, A.M.. "Evidence for a reduced chemokine response in the lungs of beige mice infected with Mycobacterium avium". Immunology 90 4 (1997): 600-606. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030899356&partnerID=MN8TOARS.
Lindblad, E.B.; Elhay, M.J.; Silva, R.; Appelberg, R.; Andersen, P.. "Adjuvant modulation of immune responses to tuberculosis subunit vaccines". Infection and Immunity 65 2 (1997): 623-629. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031020339&partnerID=MN8TOARS.
Appelberg, R.; Castro, A.G.; Silva, R.A.; Pedrosa, J.. "Cytokines involved in resistance to Mycobacterium avium in a mouse model of infection". Medical Principles and Practice 6 2 (1997): 97-102. http://www.scopus.com/inward/record.url?eid=2-s2.0-0004362454&partnerID=MN8TOARS.
Appelberg, R.. "Immune response to atypical mycobacteria". Research in Immunology 147 8-9 (1996): 560-564. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030440785&partnerID=MN8TOARS.
10.1016/S0923-2494(97)85222-4
Leal, I.S.; Appelberg, R.; Silva, M.T.. "Neutrophils are involved in the non-specific resistance to listeriosis induced by mycobacterial infections". Immunology 89 3 (1996): 442-448. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029836746&partnerID=MN8TOARS.
Sarmento, A.; Appelberg, R.. "Involvement of reactive oxygen intermediates in tumor necrosis factor alpha-dependent bacteriostasis of Mycobacterium avium". Infection and Immunity 64 8 (1996): 3224-3230. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030017333&partnerID=MN8TOARS.
Vilanova, M.; Tavares, D.; Ferreira, P.; Oliveira, L.; Nóbrega, A.; Appelberg, R.; Arala-Chaves, M.. "Role of monocytes in the up-regulation of the early activation marker CD69 on B and T murine lymphocytes induced by microbial mitogens". Scandinavian Journal of Immunology 43 2 (1996): 155-163. http://www.scopus.com/inward/record.url?eid=2-s2.0-0030053528&partnerID=MN8TOARS.
APPELBERG, R.. "Opposing Effects of Interleukin-10 on Mouse Macrophage Functions". Scandinavian Journal of Immunology 41 6 (1995): 539-544. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029034333&partnerID=MN8TOARS.
10.1111/j.1365-3083.1995.tb03605.x
CASTRO, A.G.; SILVA, R.A.; MINÓPRIO, P.; APPELBERG, R.. "In Vivo Evidence for a Non-T Cell Origin of Interleukin-5". Scandinavian Journal of Immunology 41 3 (1995): 288-292. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028954076&partnerID=MN8TOARS.
10.1111/j.1365-3083.1995.tb03566.x
Castro, A.G.; Silva, R.A.; Appelberg, R.. "Endogenously produced IL-12 is required for the induction of protective T cells during Mycobacterium avium infections in mice". Journal of Immunology 155 4 (1995): 2013-2019. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029162723&partnerID=MN8TOARS.
Appelberg, R.; Castro, A.G.; Gomes, S.; Pedrosa, J.; Silva, M.T.. "Susceptibility of beige mice to Mycobacterium avium: Role of neutrophils". Infection and Immunity 63 9 (1995): 3381-3387. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029121416&partnerID=MN8TOARS.
Sarmento, A.M.; Appelberg, R.. "Relationship between virulence of Mycobacterium avium strains and induction of tumor necrosis factor alpha production in infected mice and in in vitro-cultured mouse macrophages". Infection and Immunity 63 10 (1995): 3759-3764. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029147036&partnerID=MN8TOARS.
Castro, A.G.; Minoprio, P.; Appelberg, R.. "The relative impact of bacterial virulence and host genetic background on cytokine expression during Mycobacterium avium infection of mice". Immunology 85 4 (1995): 556-561. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029148250&partnerID=MN8TOARS.
Appelberg, R.; Sarmento, A.; Castro, A.G.. "Tumour necrosis factor-alpha (TNF-a) in the host resistance to mycobacteria of distinct virulence". Clinical and Experimental Immunology 101 2 (1995): 308-313. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029085639&partnerID=MN8TOARS.
Pedrosa, J.; Florido, M.; Kunze, Z.M.; Castro, A.G.; Portaels, F.; McFadden, J.; Silva, M.T.; Appelberg, R.. "Characterization of the virulence of Mycobacterium avium complex (MAC) isolates in mice". Clinical and Experimental Immunology 98 2 (1994): 210-216. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028062985&partnerID=MN8TOARS.
Appelberg, R.; Castro, A.G.; Pedrosa, J.; Minoprio, P.. "Role of interleukin-6 in the induction of protective T cells during mycobacterial infections in mice". Immunology 82 3 (1994): 361-364. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028103903&partnerID=MN8TOARS.
Appelberg, R.; Castro, A.G.; Pedrosa, J.; Silva, R.A.; Orme, I.M.; Minoprio, P.. "Role of gamma interferon and tumor necrosis factor alpha during T-cell- independent and -dependent phases of Mycobacterium avium infection". Infection and Immunity 62 9 (1994): 3962-3971. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028142783&partnerID=MN8TOARS.
Appelberg, R.; Castro, A.G.; Silva, M.T.. "Neutrophils as effector cells of T-cell-mediated, acquired immunity in murine listeriosis". Immunology 83 2 (1994): 302-307. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028139111&partnerID=MN8TOARS.
Appelberg, R.. "Protective role of interferon gamma, tumor necrosis factor alpha and interleukin-6 in Mycobacterium tuberculosis and M. avium infections". Immunobiology 191 4-5 (1994): 520-525. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027967447&partnerID=MN8TOARS.
Appelberg, R.; Orme, I.M.. "Effector mechanisms involved in cytokine-mediated bacteriostasis of Mycobacterium avium infections in murine macrophages". Immunology 80 3 (1993): 352-359. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027515638&partnerID=MN8TOARS.
Appelberg, R.. "Macrophage inflammatory proteins MIP-1 and MIP-2 are involved in T cell-mediated neutrophil recruitment". Journal of Leukocyte Biology 52 3 (1992): 303-306. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026671432&partnerID=MN8TOARS.
Appelberg, R.. "Mycobacterial infection primes T cells and macrophages for enhanced recruitment of neutrophils". Journal of Leukocyte Biology 51 5 (1992): 472-477. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026631652&partnerID=MN8TOARS.
Appelberg, R.. "T cell regulation of the chronic peritoneal neutrophilia during mycobacterial infections". Clinical and Experimental Immunology 89 1 (1992): 120-125. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026717745&partnerID=MN8TOARS.
Appelberg, R.. "CD4+ T cells are required for antigen-specific recruitment of neutrophils by BCG-immune spleen cells". Immunology 75 3 (1992): 414-419. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026511349&partnerID=MN8TOARS.
Appelberg, R.; Orme, I.M.; Pinto De Sousa, M.I.; Silva, M.T.. "In vitro effects of interleukin-4 on interferon-¿-induced macrophage activation". Immunology 76 4 (1992): 553-559. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026781705&partnerID=MN8TOARS.
Appelberg, R.; Pedrosa, J.. "Induction and expression of protective T cells during Mycobacterium avium infections in mice". Clinical and Experimental Immunology 87 3 (1992): 379-385. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026596268&partnerID=MN8TOARS.
Appelberg, R.. "Interferon-gamma (IFN-¿) and macrophage inflammatory proteins (MIP)-1 and -2 are involved in the regulation of the T cell-dependent chronic peritoneal neutrophilia of mice infected with mycobacteria". Clinical and Experimental Immunology 89 2 (1992): 269-273. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026684453&partnerID=MN8TOARS.
Furney, S.K.; Skinner, P.S.; Roberts, A.D.; Appelberg, R.; Orme, I.M.. "Capacity of Mycobacterium avium isolates to grow well or poorly in murine macrophages resides in their ability to induce secretion of tumor necrosis factor". Infection and Immunity 60 10 (1992): 4410-4413. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026733618&partnerID=MN8TOARS.
Kunze, Z.M.; Wall, S.; Appelberg, R.; Silva, M.T.; Portaels, F.; McFadden, J.J.. "IS901, a new member of a widespread class of atypical insertion sequences, is associated with pathogenicity in Mycobacterium avium". Molecular Microbiology 5 9 (1991): 2265-2272. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025833204&partnerID=MN8TOARS.
Appelberg, R.; Pedrosa, J.M.; Silva, M.T.. "Host and bacterial factors control the Mycobacterium avium-induced chronic peritoneal granulocytosis in mice". Clinical and Experimental Immunology 83 2 (1991): 231-236. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026073001&partnerID=MN8TOARS.
Orme, I.M.; Lee, B.Y.; Appelberg, R.; Miller, E.S.; Chi, D.L.; Griffin, J.P.; Roberts, A.D.. "T cell response in acquired protective immunity to Mycobacterium tuberculosis infection". Bulletin of the International Union Against Tuberculosis and Lung Disease 66 1 (1991): 7-13. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025829724&partnerID=MN8TOARS.
Appelberg, R.; Sarmento, A.M.. "The role of macrophage activation and of Bcg-encoded macrophage function(s) in the control of Mycobacterium avium infection in mice". Clinical and Experimental Immunology 80 3 (1990): 324-331. http://www.scopus.com/inward/record.url?eid=2-s2.0-0025333358&partnerID=MN8TOARS.
Silva, M.T.; Silva, M.NazarT.; Appelberg, R.. "Neutrophil-macrophage cooperation in the host defence against mycobacterial infections". Microbial Pathogenesis 6 5 (1989): 369-380. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024375661&partnerID=MN8TOARS.
10.1016/0882-4010(89)90079-X
Appelberg, R.; Soares, R.; Ferreira, P.; Silva, M.T.. "Induction of non-specific immunosuppression in mice by mycobacterial infections and its relationship to macrophage activation". Scandinavian Journal of Immunology 30 2 (1989): 165-174. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024383411&partnerID=MN8TOARS.
Appelberg, R.; Silva, M.T.. "T cell-dependent chronic neutrophilia during mycobacterial infections". Clinical and Experimental Immunology 78 3 (1989): 478-483. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024380858&partnerID=MN8TOARS.
Ramos, T.; Zalcberg-Quintana, I.; Appelberg, R.; Sarno, E.N.; Silva, M.T.. "T-helper cell subpopulations and the immune spectrum of leprosy". International Journal of Leprosy 57 1 (1989): 73-81. http://www.scopus.com/inward/record.url?eid=2-s2.0-0024349482&partnerID=MN8TOARS.
Silva, M.T.; Appelberg, R.; Nazaret, M.; Macedo, P.M.. "In vivo killing and degradation of Mycobacterium aurum within mouse peritoneal macrophages". Infection and Immunity 55 9 (1987): 2006-2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-0023204619&partnerID=MN8TOARS.

Activities

Supervision

	Thesis Title Role	Degree Subject (Type) Institution / Organization
2014 - 2019	Assembly of granulomas during mycobacterial infections in mice: cellular and molecular dynamics Supervisor of Mariana Rodrigues Resende da Silva	Universidade do Minho, Portugal
2014/09 - 2018/09	Unravelling the role of keratins in bacterial infections Co-supervisor of Rui Filipe da Silva e Cruz	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2014/09 - 2018/05	Molecular characterization of newly identidied Listeria monocytogenes virulence regulators Co-supervisor of Jorge Nuno Martins Campos Pinheiro	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2012 - 2016/12	T cells during mycobacterial infections: production and activation Co-supervisor	Universidade do Minho, Portugal
2008 - 2013/10	The immune response to mycobacteria of distinct virulence Supervisor of Manuel António Afonso Pereira Barroso	Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2006/10 - 2012/06	The adaptive response of mycobacterium avium to anoxia and nitric oxide Supervisor of John Edward Pearl	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2006/12 - 2011/02	Identification and charactersation of new listeria monocytogenes virulence factors. Co-supervisor of Olga Andreia dos Santos Reis
2006/03 - 2011/02	Identification of new Listeria monocytogenes virulence factors: comparative genomics and transcriptomics Co-supervisor of Ana Margarida Figueiredo e Salles Camejo	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2006/12 - 2009/06	Infection of the thymus by mycobacteria: potential impact on the immune response to infection. Co-supervisor of Cláudia Rubina Freitas Pereira de Nóbrega	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2006/02 - 2008/07	Mechanisms of early immune activation in response to hepatitis B virus. Co-supervisor of Sílvia Margarida Teixeira Vilarinho	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1999 - 2007/06	Mycobacterium avium and its adaptation to the host's immune response - importance of nitric oxide production. Supervisor of Susana Ferreira Fonseca Lousada Soares	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
2000/09 - 2007/04	Novas vias para o controlo do crescimento do Mycobacterium avium. Supervisor of Sofia Isabel Lapa Ribeiro de Sousa Fernandes	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1998/09 - 2004/02	REGULATION AND ACTIVATION OF MURINE TOLL-LIKE RECEPTORS Co-supervisor of João Pedro Antunes Gomes Pereira	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1995 - 2002/01	Modulação da resposta imunológica do ratinho à infecção pelo Mycobacterium avium: papel da IL10 e IL12 em estratégias de imunomodulação, vacinação e quimioterapia Supervisor of Regina Augusta Alves Pereira da silva	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1994/08 - 2001/09	Cytokines as potential coadjuvants for a subunit vaccine against tubercolosis Supervisor of Irene Sofia Viana Guimarães Moreira Leal	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1994/08 - 2000/09	O papel do neutrófilo na resistência à infecção por parasitas intracelulares Co-supervisor of Jorge Manuel Rolo Pedrosa	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1994 - 2000/05	Interacção Mycobacterium avium-hospedeiro: factores de virulência e mecanismos de resistência Supervisor of Maria Manuela Flórido Pereira da Costa	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1995 - 2000	Interacção do Mycobacterium avium com o macrófago em apoptose Supervisor of Maria Teresa Albuquerque Santos Faria Pais	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal
1994 - 1999	A interação entre o Mycobacterium avium e o macrófago Supervisor of Maria Salomé Custódio Gomes	Universidade do Porto Instituto de Ciências Biomédicas Abel Salazar, Portugal

Distinctions

Award

1997	1º prémio Pfizer Pfizer Portugal, Portugal Sociedade de Ciências Médicas de Lisboa, Portugal
1994	1º prémio Pfizer Pfizer Portugal, Portugal Sociedade de Ciências Médicas de Lisboa, Portugal
1991	1º prémio Pfizer Pfizer Portugal, Portugal Sociedade de Ciências Médicas de Lisboa, Portugal