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Rita Perfeito received her PhD in Cell Biology with top marks in 2012 from the University of Coimbra (3rd in PT rank). Her PhD project clarified the interplay between a-synuclein and several mechanisms underlying Parkinson's Disease pathology, particularly mitochondrial dysfunction and intracellular antioxidant defence impairment. In 2014, R. Perfeito moved to the Gene and Cell Therapies for the Brain group, headed by Prof. Pereira de Almeida, at CIBB (Center for Innovative Biomedicine and Biotechnology), to work as a postdoctoral researcher. Here, she had the great opportunity to meet different research approaches, as the group is focused not only on disclosing disease mechanisms but also on developing innovative therapeutic methodologies (mainly through gene therapy and genetic edition) for the treatment of neurodegenerative disorders. In line with this, Rita decided to apply to the very competitive FCT call for postdoctoral fellowships, which she was awarded in 2015. During these years, R. Perfeito enrolled in several national and international projects as a team member, was awarded as Principal Investigator by NAF in 2020 and recently by Fundação para a Ciência e a Tecnologia (2022) to develop studies in gene therapy targeting Machado-Joseph disease. In 2023, Rita was hired Assistant Researcher by the University of Coimbra, under the project "CinTech" - Technological Hub for Innovation, Translation and Industrialization of Complex Injectable Drugs, funded by PRR and the European Union. Currently, Rita is author/co-author of more than 20 papers/book chapters/abstracts published in peer-reviewed international journals and has participated in several national and international meetings with oral and/or poster presentations. In the last 5 years, R. Perfeito has (co)supervised 4 master theses, integrated several jury committees and collaborated in supervising numerous graduate and undergraduate students. At present, she is also supervising a PhD student, who was awarded a PhD scholarship by FCT in 2022. As an important component of her scientific career, Rita Perfeito has extensively contributed as a lecturer in MSc and PhD programmes of the University of Coimbra and others. Furthermore, she has also participated in diverse science communication activities integrated into outreach programmes, including interviews, articles in newspapers and multimedia content development. PLEASE BE AWARE that Rita had two career breaks (in 2014 and 2017) due to pregnancies and the respective periods of maternity leave. Furthermore, R. Perfeito has also suspended her lab work for several months in 2020 and 2021 due to the COVID19 pandemic, as to the imperative need to give support to her young children 4 and 7 years old, who stayed without school during the restrictions and lock-downs imposed by the Portuguese Government. All these interruptions seriously impact her scientific productivity.
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Identification

Personal identification

Full name
Rita Catarina Gonçalves Perfeito

Citation names

  • Perfeito, Rita
  • Perfeito, R.
  • Rita Perfeito

Author identifiers

Ciência ID
5E11-9030-F74B

Email addresses

  • rita.perfeito@uc.pt (Professional)
  • rperfeito@cnc.uc.pt (Professional)

Addresses

  • Centro de Neurociências e Biologia Celular. Rua Larga, Faculdade de Medicina, pólo I, Universidade de Coimbra, 3004-504, Coimbra, Coimbra, Portugal (Professional)

Websites

Knowledge fields

  • Medical and Health Sciences - Basic Medicine - Neurosciences
  • Medical and Health Sciences - Medical Biotechnology - Gene-based Diagnostics and Therapeutic Interventions
  • Medical and Health Sciences - Basic Medicine
  • Medical and Health Sciences - Health Sciences
  • Natural sciences - Biological Sciences - Cell Biology
Education
Degree Classification
2023/02/08 - 2023/12
Ongoing
 Clinical Investigator Certificate (CLIC) (Pós-Graduação)
Universidade de Aveiro, Portugal

Universidade Nova de Lisboa Comprehensive Health Research Centre, Portugal

Portuguese Clinical Research Infrastructure Network, Portugal

Universidade de Aveiro Associação para a Formação Profissional e Investigação, Portugal
2015/07/01 - 2019/01/31
Concluded
 Role and Mechanisms of alpha-synuclein spreading in Parkinson's disease (Pós-doutoramento)
Major in Neurociências
Centro de Neurociências e Biologia Celular, Portugal
 N/A
2014/09/01 - 2015/06/30
Concluded
 New strategies to manage brain diseases (Pós-doutoramento)
Major in Neurociências
Universidade de Coimbra, Portugal
 N/A
2014/01/02 - 2014/08/31
Concluded
 Cell to cell transport of alpha-synuclein in Parkinson's Disease. The progression factor? (Pós-doutoramento)
Major in Neurociências
Centro de Neurociências e Biologia Celular, Portugal
 N/A
2012/12/28
Concluded
 Biologia (Doutoramento)
Major in Biologia Celular
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Interplay between alpha-synuclein and oxidative stress in Parkinson's disease cell models." (THESIS/DISSERTATION)
 aprovado com louvor e distinção por unanimidade
2005
Concluded
 Biologia (Licenciatura)
Major in ´Ramo de investigação
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
"Lesão oxidativa de Oligodendrócitos - Protecção por antioxidantes e insulina" (THESIS/DISSERTATION)
 15 (out of 20)
Affiliation

Science

Category
Host institution 		Employer
2023/09/01 - Current Research Assistant (Research) Universidade de Coimbra, Portugal
Centro de Inovação em Biomedicina e Biotecnologia (CIBB), Portugal
2019/02/01 - 2023/08/31 Contracted Researcher (Research) Centro de Neurociências e Biologia Celular, Portugal
Centro de Inovação em Biomedicina e Biotecnologia (CIBB), Portugal
(...)
2015/07/01 - 2019/01/31 Postdoc (Research) Fundação para a Ciência e a Tecnologia, Portugal
Centro de Neurociências e Biologia Celular, Portugal
2014/09/01 - 2015/06/30 Postdoc (Research) Universidade de Coimbra, Portugal
Centro de Neurociências e Biologia Celular, Portugal
2014/01/02 - 2014/08/31 Postdoc (Research) Centro de Neurociências e Biologia Celular, Portugal
2010/06/01 - 2013/01/31 Researcher (Research) Centro de Neurociências e Biologia Celular, Portugal
2006/06/01 - 2010/05/31 Researcher (Research) Fundação para a Ciência e a Tecnologia, Portugal
Centro de Neurociências e Biologia Celular, Portugal
2004/09 - 2005/07 Research Trainee (Research) Centro de Neurociências e Biologia Celular, Portugal
Centro de Neurociências e Biologia Celular, Portugal
2005/04/01 - 2005/04/30 Visiting Researcher (Research) Eidgenossische Technische Hochschule Zürich Campus Hönggerberg, Switzerland
Eidgenossische Technische Hochschule Zürich Campus Hönggerberg, Switzerland

Others

Category
Host institution 		Employer
2017/09/04 - 2018/03/04 Maternity Fundação para a Ciência e a Tecnologia, Portugal
Centro de Neurociências e Biologia Celular, Portugal
2014/04/08 - 2014/09/08 Maternity Centro de Neurociências e Biologia Celular, Portugal
Projects

Grant

Designation Funders
2015/07/01 - 2019/01/31 Role and mechanisms of alpha-synuclein spreading in Parkinson's disease
SFRH/BPD/100130/2014
Post-doc Fellow
Centro de Neurociências e Biologia Celular, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2006/06/01 - 2010/05/31 Interplay between alpha-synuclein and oxidative stress in Parkinson's disease cell models
SFRH/BD/25515/2005
PhD Student Fellow
Universidade de Coimbra, Portugal

Centro de Neurociências e Biologia Celular, Portugal
Fundação para a Ciência e a Tecnologia, I.P.
Concluded

Contract

Designation Funders
2023/09/01 - Current CinTech - Technological Hub for Innovation, Translation and Industrialization of Complex Injectable Drugs
12345
Researcher
Centro de Inovação em Biomedicina e Biotecnologia (CIBB), Portugal
Ongoing
2023/03/01 - Current Pre-clinical evaluation of the brain-targeting AAV-PHP.eB encoding cholesterol hydroxylase CYP46A1 as a therapeutic strategy for spinocerebellar ataxia type 3 - towards the understanding of the neuroprotective mechanism
2022.04788.PTDC
Principal investigator
Centro de Inovação em Biomedicina e Biotecnologia (CIBB), Portugal

Universidade de Coimbra, Portugal
Fundação para a Ciência e a Tecnologia, I.P.
Ongoing
2023/01/01 - Current Conversão Directa de Astrócitos em Neurónios Cerebelares como tratamento Inovador para a Ataxia Espinocerebelosa do Tipo-3
2022.06127.PTDC
Researcher
Centro de Inovação em Biomedicina e Biotecnologia (CIBB), Portugal

Universidade de Coimbra, Portugal
Fundação para a Ciência e a Tecnologia, I.P.
Ongoing
2021/01/01 - 2025/12/31 Centro de Inovação em Biomedicina e Biotecnologia - Laboratório Associado
LA/P/0058/2020
Researcher
CNC.IBILI, Portugal
Ongoing
2020/01/01 - 2023/12/31 Centro de Inovação em Biomedicina e Biotecnologia
UIDB/04539/2020
Researcher
CNC.IBILI, Portugal
Cancelled
2019/01 - 2022/12/31 Gene Therapy for Cerebellar Ataxias: restoring cholesterol metabolism by targeting brain cholesterol 24 hydroxylase (CYP46A1) - SCA-CYP (E-RARE International Consortium)
E-Rare-3 JTC 2017
Researcher
CNC.IBILI, Portugal
Ongoing
2020/03 - 2022/12 Intravenous delivery of the brain-targeting AAV-PHPeB encoding the cholesterol hydroxylase CYP46A1 into a mouse model of spinocerebellar ataxia type 3: a promising non-invasive therapeutic strategy
686213
Principal investigator
2018/06 - 2022/06/14 The impact of induced pluripotent stem cells-derived neuroepithelial stem cells transplantation in Machado-Joseph disease
PTDC/BTM-ORG/30737/2017
Researcher
CNC.IBILI, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Ongoing
2018/07 - 2021/06 Role of microRNA deregulation in Machado-Joseph disease: on the development of a microRNA-based therapeutic strategy
PTDC/MED-NEU/32309/2017
Researcher
CNC.IBILI, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Ongoing
2017/01 - 2020/12 Brain Health 2020
CENTRO-01-0145-FEDER-000008
Researcher
CNC.IBILI, Portugal
Ongoing
2020/08/01 - 2020/10/31 Verão com Ciência: Metodologias de Investigação Científica - I&D: Terapia Génica e Células Estaminais
MIC IIIUC
Researcher
Universidade de Coimbra, Portugal

Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
Fundação para a Ciência e a Tecnologia
Concluded
2016/05 - 2019/04 Control of cardiomyocyte proliferation in disease and regenerative medicine
PTDC/BIM-MED/3363/2014
Researcher
CNC.IBILI, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2015/03/01 - 2018/12 Roles and mechanisms of synuclein and ataxin-3 spreading in Parkinson and Machado-Joseph diseases - SynSpread (JPND - International Consortium)
JPND SynSpread: CD/0001/2013
Researcher
Centro de Neurociências e Biologia Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2015/01 - 2018/12 CNC.IBILI
UID/NEU/04539/2013
Researcher
CNC.IBILI, Portugal
 Fundação para a Ciência e a Tecnologia, I.P.
Concluded
2016/01 - 2016/12 Does the transplantation of mutant ataxin-3-depleted patient-derived NSC alleviates Machado Joseph disease (MJD)?
20126
Researcher
CNC.IBILI, Portugal
 AFM-Téléthon
Concluded
2015/01 - 2015/12 The transplantation of induced pluripotent stem cells (iPSC)-derived neural stem cells (NSC) in Machado-Joseph disease (MJD)
Research Seed Money Award
Researcher
Centro de Neurociências e Biologia Celular, Portugal
 National Ataxia Foundation
Concluded
2014/09/01 - 2015/06/30 New strategies to manage brain diseases
CENTRO-07-ST24FEDER-002002
Post-doc Fellow
Centro de Neurociências e Biologia Celular, Portugal

Universidade de Coimbra, Portugal
Universidade de Coimbra
Concluded
2014/06/01 - 2014/08/31 Cell to cell transport of alpha-synuclein in Parkinson's disease. The progression factor?
PTDC/SAU-NMC/109955/2009
Post-doc Fellow
Centro de Neurociências e Biologia Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2010/06/01 - 2013/01/31 Deciphering the molecular mechanisms linking alpha-synuclein dysfunction and cytotoxicity in Parkinson's disease - the role of GDNF
Provided by PTCRIS: 101928
Research Technician Fellow
Centro de Neurociências e Biologia Celular, Portugal
 Fundação para a Ciência e a Tecnologia
Concluded
2011/10 - 2012/07 Alpha-synuclein modifications in peripheral blood lymphocytes from sporadic and familial Parkinson's disease patients
PDCR02
Researcher
Centro de Neurociências e Biologia Celular, Portugal
 Faculdade de Medicina, Universidade de Coimbra
Concluded
Outputs

Publications

Book chapter
  1. Mendonça, L.S.; Onofre, I.; Miranda, C.O.; Perfeito, R.; Nóbrega, C.; de Almeida, L.P.. "Stem Cell-Based Therapies for Polyglutamine Diseases". In Protein misfolding in Biology and Disease, edited by Luís Pereira de Almeida; Clévio Nóbrega, 439-466. Springer, 2018.
    Published • 10.1007/978-3-319-71779-1_21
  2. Perfeito, Rita; Doutora Ana Cristina Carvalho Rego. "Post-translational modifications of alpha-synuclein and mitochondrial dysfunction in Parkinson's disease". In Protein misfolding in Biology and Disease, edited by Tiago Flemming Outeiro, 203-230. Transworld Research Network, 2008.
Conference abstract
  1. Beatriz Machado Serra; Duarte, Sónia; Nathalie Cartier; Sandro Alves; Pereira de Almeida, Luis ; Rita Perfeito. Corresponding author: Rita Perfeito. "Systemic delivery of the brain-targeting AAV-PHPeB encoding the cholesterol hydroxylase CYP46A1 into a mouse model of Machado-Joseph disease: a promising therapeutic strategy". Paper presented in 29th Annual Congress of the European-Society-of-Gene-and-Cell-Therapy (ESCGT), Edinburgh, 2022.
    Published
  2. David Rufino-Ramos; Vitor Carmona; Inês M. Martins; M. Barreira; Patrícia R. Albuquerque; Rosário Faro; Perfeito, Rita; Rui Jorge Nobre; Luís Pereira de Almeida. "Extracellular Vesicles for therapy of spinocerebellar ataxia type 3". Paper presented in ESGCT 27th Annual Congress, Barcelona, 2019.
    Published
  3. Perfeito, Rita; Vanessa Anjos; Rui Jorge Nobre; Manuel Garrido; Jens C. Schwamborn; Luís Pereira de Almeida. "Intersection of autophagy and exosome secretion on alpha-synuclein spreading pattern in in vitro models of Parkinson's disease". Paper presented in 53rd Annual Meeting of the European Society of Clinical Investigation (ESCI2019), Coimbra, 2019.
    Published
  4. Perfeito, Rita; Catarina Resende de Oliveira; Ana Cristina Carvalho Rego. "Linking alpha-synuclein phosphorylation at Ser129 to reactive oxygen species formation in SH-SY5Y cells". Paper presented in 7th FENS Forum for European Neuroscience, Amsterdam, 2010.
    Published
Conference poster
  1. David Rufino-Ramos; Kevin Leandro; Vitor Carmona; Inês Martins; Albuquerque, Patrícia R.; Ana Rita Fernandes; Henriques, Carina; et al. "Extracellular Vesicle-Based Delivery of Silencing Sequences for the Treatment of Machado-Joseph Disease | Spinocerebellar Ataxia Type-3". Paper presented in ASGCT 26th Annual Meeting, 2023.
  2. Serra, Beatriz; Duarte, Sónia; Françoise, PIGUET; Nathalie Cartier; Alves, Sandro; de Almeida, Luis Pereira; Perfeito, Rita. "Validation of the brain-targeting AAV- PHP.eB encoding cholesterol hydroxylase CYP46A1: a promising non-invasive therapeutic strategy for Spinocerebellar Ataxia Type 3". Paper presented in FENS Regional Meeting 2023, 2023.
  3. Beatriz Machado Serra; Sónia Duarte; Françoise, PIGUET; Sandro Alves; Nathalie Cartier; Luís Pereira de Almeida; Rita Perfeito. "Restoring brain cholesterol turnover as a therapeutic strategy for Spinocerebellar Ataxia type-3: non-invasive delivery of the brain-targeting AAV-PHP.eB encoding the cholesterol hydroxylase CYP46A1". Paper presented in BEB Day 2023, 2023.
  4. D. Rufino-Ramos; Kevin Leandro; Patrícia Albuquerque; Vitor Carmona; Maria Inês Martins; Faro, Rosario; Carina Henriques; et al. "Extracellular vesicle-based delivery of silencing sequences for the treatment of Machado-Joseph disease (MJD) / Spinocerebellar Ataxia Type 3 (SCA3)". Paper presented in 29th Meeting of the European Society for Gene and Cell Therapy (ESGCT), 2022.
  5. Beatriz Machado Serra; Sónia P. Duarte; Nathalie Cartier; Sandro Alves; de Almeida, Luis Pereira; Rita Perfeito. Corresponding author: Rita Perfeito. "Systemic delivery of the brain-targeting AAV-PHP.eB encoding the cholesterol hydroxylase CYP46A1 into a mouse model of Machado-Joseph disease: a promising therapeutic strategy". Paper presented in 29th Meeting of the European Society for Gene and Cell Therapy (ESGCT), 2022.
  6. Serra, Beatriz; Sónia P. Duarte; Nathalie Cartier; Sandro Alves; de Almeida, Luis Pereira; Rita Perfeito. Corresponding author: Rita Perfeito. "Intravenous delivery of the brain-targeting AAV-PHPeB encoding the cholesterol hydroxylase CYP46A1 into a mouse model of spinocerebellar ataxia type 3: a promising non-invasive therapeutic strategy". Paper presented in XVII Meeting of the Portuguese Society for Neuroscience, 2021.
  7. Rufino-Ramos, David; Kevin Leandro; Vitor Carmona; Inês M. Martins; Manuela Paolleta; Patrícia R. Albuquerque; Rosário Faro; et al. "Intranasal delivery of extracellular vesicles carrying silencing sequences alleviates Machado-Joseph Disease". Paper presented in XVII Meeting of the Portuguese Society for Neuroscience, 2021.
  8. Perfeito, Rita; Ohein, M; Jens C. Schwamborn; Fleming, R; Luís Pereira de Almeida. "Roles and Mechanisms of alpha-synuclein and ataxin-3 spreading in Parkinson and Machado Joseph diseases - SynSpread". Paper presented in JPND/JPco-funND Conference (Brussels - Belgium), 2019.
  9. Andreia Marques; David Rufino-Ramos; Carina Henriques; Jens C. Schwamborn; Perfeito, Rita; Luís Pereira de Almeida. "Intercellular transport of alpha-synuclein in the context of Parkinson's disease". Paper presented in 1st Annual Meeting in Pharma (Coimbra, Portugal), 2019.
  10. Rita Perfeito; Vanessa Anjos; Andreia Marques; Rui Jorge Nobre; Manuel Garrido; Jens C. Schwamborn; Luís Pereira de Almeida. "Intersection of autophagy and exosome secretion on alpha-synuclein spreading profile in Parkinson's disease cell models". Paper presented in XVI Meeting of the Portuguese Society for Neuroscience (Lisboa, Portugal), 2019.
  11. L. Gaspar; M. Santana; Rita Perfeito; P. Albuquerque; T. M. Ribeiro-Rodrigues; H. Girão; R. J. Nobre; L. P. de Almeida. "Optimization of a size exclusion chromatography protocol to isolate plasma-derived extracellular vesicles for transcriptional biomarkers research". Paper presented in ISEV Annual Meeting (Barceloma, Spain), 2018.
  12. D. Rufino-Ramos; V. Carmona; P. R. Albuquerque; Rita Perfeito; R. J. Nobre; T. M. Ribeiro-Rodrigues; H. Girão. "EVs as natural carriers of miRNAs against Machado-Joseph disease". Paper presented in 1st Meeting on Vesicular Biology (Coimbra, Portugal), 2018.
  13. M. Santana; L. Gaspar; Rita Perfeito; P. Albuquerque; T. M. Ribeiro-Rodrigues; H. Girão; R. J. Nobre; L. P. de Almeida. "Optimization of a size exclusion chromatography protocol to isolate plasma-derived extracellular vesicles for transcriptional analysis". Paper presented in 1st Meeting on Vesicular Biology (Coimbra, Portugal), 2018.
  14. David Rufino-Ramos; Vitor Carmona; Patrícia R. Albuquerque; Rita Perfeito; Rui J. Nobre; Luís P. de Almeida. "Novel promising vesicles carrying artificial miRNAs against Machado-Joseph disease". Paper presented in XII Spanish-Portuguese Conference on Controlled Drug Delivery (Coimbra, Portugal), 2018.
  15. David Rufino-Ramos; Vitor Carmona; Patrícia Albuquerque; Rita Perfeito; Rui J. Nobre; Luís P. de Almeida. "Extracellular vesicles as emergent natural carriers of small nucleic acids". Paper presented in MicroBiotec (Porto, Portugal), 2017.
  16. Rita Perfeito; Vanessa Anjos; Rui J. Nobre; Manuel Garrido; Luís P. de Almeida. "Alpha-synuclein secretion in in vitro models of Parkinson's disease - a role for autophagy". Paper presented in XV Meeting of the Portuguese Society for Neuroscience (Braga, Portugal), 2017.
  17. Rita Perfeito. "Autophagy activation prevents alpha-synuclein secretion in exosomes". Paper presented in Annual Meeting - ISEV (Rotterdam, The Netherlands), 2016.
  18. Rita Perfeito. "Alpha-synuclein is transported and induces neuropathological and motor behaviour abnormalities upon overexpression in the rat striatum". Paper presented in XIV Meeting of the Portuguese Society for Neuroscience (Póvoa do Varzim, Portugal), 2015.
  19. Rita Perfeito. "Generation of a genetic model of Parkinson's disease based on alpha-synuclein overexpression in the striatum". Paper presented in XII Annual CNC Meeting, 2014.
  20. Rita Perfeito. "Interplay between oxidative stress and alpha-synuclein phosphorylation in Parkinson's disease cell models". Paper presented in Society for Neuroscience (New Orleans, USA), 2012.
  21. Rita Perfeito. "Oxidative stress in SH-SY5Y cells conditionally overexpressing wild-type alpha-synuclein". Paper presented in 8th FENS Forum of European Neuroscience (Barcelona, Spain), 2012.
  22. Rita Perfeito. "Effects of alpha-synuclein overexpression in cellular response to oxidative stress - insights for Parkinson's disease". Paper presented in 26ª reunião do GEECD (Tomar, Portugal), 2012.
  23. Rita Perfeito. "Overexpression of wild-type alpha-synuclein increases susceptibility to oxidative stress in SH-SY5Y cells". Paper presented in International Symposium on Redox Signaling and Oxidative stress in Health and Disease (Valencia, Spain), 2012.
  24. Rita Perfeito. "Wild-type alpha-synuclein overexpression modifies nuclear and mitochondrial transcription factors". Paper presented in 5th Annual Meeting of Cell Signaling (Coimbra, Portugal), 2011.
  25. Rita Perfeito. "Alpha-synuclein phosphorylation at Serine 129 is induced by oxidative stress and influences its secretion in a Parkinson's disease cellular model". Paper presented in The 10th International Conference on Alzheimer's and Parkinson's disease (ADPD) (Barcelona, Spain), 2011.
  26. Rita Perfeito. "Iron-induced oxidative stress and rotenone modify alpha-synuclein phosphorylation in a cellular model of Parkinson's disease". Paper presented in 11th Meeting of the Portuguese Society for Neuroscience (Braga, Portugal), 2009.
  27. Rita Perfeito. "Influence of iron and rotenone on alpha-synuclein post-translational modifications in cellular models of Parkinson's disease". Paper presented in 6th FENS Forum of European Neuroscience (Geneve - Switzerland), 2008.
  28. Rita Perfeito. "Mitochondrial dysfunction and Cell Toxicity in Parkinson's disease models". Paper presented in 10th Meeting of the Portuguese Society for Neuroscience (Ofir - Portugal), 2007.
Journal article
  1. Rufino-Ramos, David; Albuquerque, Patrícia R.; Leandro, Kevin; Carmona, Vitor; Martins, Inês M.; Fernandes, Rita; Henriques, Carina; et al. "Extracellular vesicle-based delivery of silencing sequences for the treatment of Machado-Joseph disease/spinocerebellar ataxia type 3". Molecular Therapy 31 5 (2023): 1275-1292. http://dx.doi.org/10.1016/j.ymthe.2023.04.001.
    10.1016/j.ymthe.2023.04.001
  2. Walter, Jonas; Bolognin, Silvia; Poovathingal, Suresh K.; Magni, Stefano; Gérard, Deborah; Antony, Paul M.A.; Nickels, Sarah L.; et al. "The Parkinson's-disease-associated mutation LRRK2-G2019S alters dopaminergic differentiation dynamics via NR2F1". Cell Reports 37 3 (2021): 109864. http://dx.doi.org/10.1016/j.celrep.2021.109864.
    Published • 10.1016/j.celrep.2021.109864
  3. Walter, Jonas; Bolognin, Silvia; Antony, Paul M.A.; Nickels, Sarah L.; Poovathingal, Suresh K.; Salamanca, Luis; Magni, Stefano; et al. "Neural Stem Cells of Parkinson's Disease Patients Exhibit Aberrant Mitochondrial Morphology and Functionality". Stem Cell Reports 12 5 (2019): 878-889. http://dx.doi.org/10.1016/j.stemcr.2019.03.004.
    Published • 10.1016/j.stemcr.2019.03.004
  4. Perfeito, Rita; Anjos, Vanessa; Marques, Andreia; Nobre, Rui; Garrido, Manuel; Schwamborn, Jens; De Almeida, Luís. "Intersection of exosome secretion and autophagy on alpha-synuclein spreading profile in Parkinson's disease cell models". Frontiers in Cellular Neuroscience 13 (2019): http://dx.doi.org/10.3389/conf.fncel.2019.01.00015.
    Published • 10.3389/conf.fncel.2019.01.00015
  5. Laetitia Gaspar; Magda Santana; Perfeito, Rita; Patrícia Albuquerque; Teresa M. Ribeiro Rodrigues; Henrique Girão; Rui Jorge Nobre; Luís Pereira de Almeida. "Optimization of a size-exclusion chromatograhy protocol to isolate plasma-derived extracellular vesicles". Journal of Extracellular Vesicles 7 sup1 (2018): 1461450. http://dx.doi.org/10.1080/20013078.2018.1461450.
    Published • 10.1080/20013078.2018.1461450
  6. Noronha, C.; Perfeito, R.; Laço, M.; Wüllner, U.; Rego, A.C.. "Expanded and Wild-type Ataxin-3 Modify the Redox Status of SH-SY5Y Cells Overexpressing a-Synuclein". Neurochemical Research 42 5 (2017): 1430-1437. http://www.scopus.com/inward/record.url?eid=2-s2.0-85013849882&partnerID=MN8TOARS.
    Published • 10.1007/s11064-017-2199-7
  7. Perfeito, R.; Ribeiro, M.; Rego, A.C.. "Alpha-synuclein-induced oxidative stress correlates with altered superoxide dismutase and glutathione synthesis in human neuroblastoma SH-SY5Y cells". Archives of Toxicology 91 3 (2017): 1245-1259. http://www.scopus.com/inward/record.url?eid=2-s2.0-84978795483&partnerID=MN8TOARS.
    Published • 10.1007/s00204-016-1788-6
  8. Rufino-Ramos, D.; Albuquerque, P.R.; Carmona, V.; Perfeito, R.; Nobre, R.J.; Pereira de Almeida, L.. "Extracellular vesicles: Novel promising delivery systems for therapy of brain diseases". Journal of Controlled Release 262 (2017): 247-258. http://www.scopus.com/inward/record.url?eid=2-s2.0-85026758579&partnerID=MN8TOARS.
    Published • 10.1016/j.jconrel.2017.07.001
  9. Perfeito, Rita; Vanessa Anjos; Rui Jorge Nobre; Manuel Garrido; Luís Pereira de Almeida. "Autophagy activation prevents alpha-synuclein secretion in exosomes". Journal of Extracellular Vesicles 5 1 (2016): 31552. http://dx.doi.org/10.3402/jev.v5.31552.
    Published • 10.3402/jev.v5.31552
  10. Perfeito, R.; Lázaro, D.F.; Outeiro, T.F.; Rego, A.C.. "Linking alpha-synuclein phosphorylation to reactive oxygen species formation and mitochondrial dysfunction in SH-SY5Y cells". Molecular and Cellular Neuroscience 62 (2014): 51-59. http://www.scopus.com/inward/record.url?eid=2-s2.0-84906751686&partnerID=MN8TOARS.
    Published • 10.1016/j.mcn.2014.08.002
  11. Perfeito, R.; Cunha-Oliveira, T.; Rego, A.C.. "Reprint of: Revisiting oxidative stress and mitochondrial dysfunction in the pathogenesis of Parkinson disease - Resemblance to the effect of amphetamine drugs of abuse". Free Radical Biology and Medicine 62 (2013): 186-201. http://www.scopus.com/inward/record.url?eid=2-s2.0-84884889978&partnerID=MN8TOARS.
    Published • 10.1016/j.freeradbiomed.2013.05.042
  12. Perfeito, R.; Rego Prof., A.C.. "Role of alpha-synuclein and Parkinson's disease-associated mitochondrial dysfunction,Papel da alfa-sinucleína e da disfunção mitocondrial associada à doença de Parkinson". Revista Neurociencias 20 2 (2012): 273-284. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863884807&partnerID=MN8TOARS.
    Published
  13. Perfeito, R.; Cunha-Oliveira, T.; Rego, A.C.. "Revisiting oxidative stress and mitochondrial dysfunction in the pathogenesis of Parkinson disease - Resemblance to the effect of amphetamine drugs of abuse". Free Radical Biology and Medicine 53 9 (2012): 1791-1806. http://www.scopus.com/inward/record.url?eid=2-s2.0-84867397192&partnerID=MN8TOARS.
    Published • 10.1016/j.freeradbiomed.2012.08.569
  14. Perfeito, R.; Oliveira, C.; Rego, A.C.. "Alpha-synuclein phosphorylation mediated by iron and rotenone in a cellular model of Parkinson's disease". Parkinsonism & Related Disorders 15 P3.011 (2009): S150. http://dx.doi.org/10.1016/s1353-8020(09)70575-9.
    Published • 10.1016/s1353-8020(09)70575-9
  15. Rita Perfeito; Catarina Resende de Oliveira; Ana Cristina Carvalho Rego. "Iron-Induced Oxidative Stress and Rotenone Modify Alpha-synuclein Phosphorylation in a Cellular Model of Parkinson's Disease". Frontiers in Neuroscience 3 (2009): http://dx.doi.org/10.3389/conf.neuro.01.2009.11.061.
    Published • 10.3389/conf.neuro.01.2009.11.061
  16. Perfeito, R.; Pereira, J.; Oliveira, C.; Bettencourt-Relvas, J.; Rego, A.C.. "Trolox protection of myelin membrane in hydrogen peroxide-treated mature oligodendrocytes". Free Radical Research 41 4 (2007): 444-451. http://www.scopus.com/inward/record.url?eid=2-s2.0-33947493269&partnerID=MN8TOARS.
    Published • 10.1080/10715760601134442
Newspaper article
  1. Luís Pereira de Almeida; André Caetano; Rita Perfeito; João Cardoso; Correia, Marta Quatorze; Sara Varela Amaral. "Terapia génica: uma chave para o tratamento de doenças raras?", Jornal Público, 2021, https://cnc.uc.pt/pt/resources.
  2. Ana Catarina Vinhas; Ana Cristina Ferreira; Carina Henriques; Carolina Silva; Catarina Oliveira Miranda; Daniel Henriques; Daniela Oliveira; et al. "Uma Viagem Rara", PÚBLICO, 2020, https://www.publico.pt/2020/02/28/ciencia/fotogaleria/viagem-rara-400481.
Thesis / Dissertation
  1. Perfeito, Rita. "Interplay between alpha-synuclein and oxidative stress in Parkinson's disease cell models". PhD, Universidade de Coimbra, 2012.
  2. Perfeito, Rita. "Protection from oxidative lesion in rat oligodendrocytes". Degree, Universidade de Coimbra Faculdade de Ciencias e Tecnologia, 2005.

Artistic / Performance

Script
  1. Carolina Simões; Rita Perfeito; Sónia Duarte; João Cardoso; Luís Pereira de Almeida. Vale a pena financiar a investigação em doenças raras? - Conteúdo Multimédia "A Saúde no Saber". 2021.
Activities

Oral presentation

Presentation title Event name
Host (Event location)
2023/02/16 Doenças Neurodegenerativas: quais são e suas principais características CURSO PARA A COMUNIDADE_Doença de Alzheimer: Desafios e Prevenção
Universidade Sénior de Oliveira do Bairro (Oliveira do Bairro, Portugal)
2022/06/08 Non-invasive delivery of the brain-targeting AAV-PHP.eB encoding the cholesterol hydroxylase CYP46A1 into a mouse model of SCA3: a promising therapeutic strategy? dcv.talks
Departamento de Ciências da Vida; Universidade de Coimbra (Coimbra, Portugal)
2022/05/28 Extracellular Vesicles carrying silencing sequences alleviate Machado-Joseph Disease (MJD) ISEV2022 Annual Meeting
International Society for Extracellular Vesicles (Lyon, France)
2022/04/02 Non-invasive delivery of the brain-targeting AAV-PHP.eB encoding the cholesterol hydroxylase CYP46A1 into a mouse model of spinocerebellar ataxia type 3: a promising therapeutic strategy Congresso SPDMov 2022
Sociedade Portuguesa de Doenças do Movimento (Luso)
2022/02/10 Intranasal delivery of Extracellular Vesicles carrying silencing sequences alleviates Machado-Joseph Disease (MJD) SPF Meeting 2022
Sociedade Portuguesa de Farmacologia (Porto, Portugal)
2022/02/09 Validation of the brain-targeting AAV-PHP.eB encoding cholesterol hydroxylase CYP46A1 as a therapeutic candidate for Spinocerebellar Ataxia type-3 SPF Meeting 2022
Sociedade Portuguesa de Farmacologia (Porto, Portugal)
2021/03/04 Gene Therapy for Neurodegenerative Disorders using a non-invasive therapeutic strategy - the case of Machado-Joseph disease. XVI Encontro Nacional de Estudantes de Engenharia Biomédica
(Coimbra, Portugal)
2019/05/22 Intersection of autophagy and exosome secretion on alpha-synuclein spreading pattern in in vitro models of Parkinson's disease 53rd Annual Scientific Meeting of the European Society for Clinical Investigation (ESCI)
(Coimbra, Portugal)
2019/02/06 Exosomes as a delivery system for miRNAs in Machado-Joseph disease XLIX Reunião da Sociedade Portuguesa de Farmacologia
SPF (Porto, Portugal)
2019/01/24 Doença de Parkinson e Envelhecimento: muito além dos sintomas motores Conferências na UNISOB
Universidade Sénior de Oliveira do Bairro (Oliveira do Bairro, Portugal)
2018/10/09 Patient-specific iPSC-derived neuroepithelial stem cells for neuroprotection and neuroregeneration in Machado Joseph disease 10th International Symposium on Neuroprotection and Neurorepair
(Dresden, Germany)
2018/04/19 A doença de Parkinson: diferentes mecanismos, a mesma (dis)função Conferências no IEC
Instituto de Educação e Cidadania (Mamarrosa, Portugal)
2018/02/16 Extracellular vesicles in brain disorders 1st Meeting on Vesicular Biology
(Coimbra, Portugal)
2017/03/17 Interplay between autophagy and alpha-synuclein spreading in in vitro models of Parkinson's disease New Challenges in the Diagnosis and Treatment of Movement Disorders
Sociedade Portuguesa de Doenças do Movimento (Ofir, Portugal)
2017/03/13 Oxidative stress in cellular models of Parkinson's disease CIMQ'17, Mentes que cambian el mundo - Journeys in the Investigation of Parkinson's disease
Universidade de Santiago de Compostela (Santiago de Compostela, Spain)
2016/02/04 Autophagy activation prevents alpha-synuclein secretion in exosomes XLVI Reunião Anual da Sociedade Portuguesa de Farmacologia
SPF (Porto, Portugal)
2015/09/30 Activating autophagy prevents alpha-synuclein secretion in exosomes 1st Iberian Meeting on Extracellular Vesicles
GEIVEX (Porto, Portugal)
2013/05/30 Alpha-synuclein overexpression modifies the antioxidant response in SH-SY5Y neuroblastoma cells XIII Reunião da Sociedade Portuguesa de Neurociências
SPN (Luso, Portugal)
2013/03/22 Interplay between alpha-synuclein and oxidative stress in Parkinson's disease cell models Congresso SPDMov
SPDMOv (Vimeiro, Portugal)
2009/12/21 Reactive oxygen species formation mediate increased alpha-synuclein phosphorylation in a cellular model of Parkinson's disease VII CNC Meeting; Session: Neuroscience and Disease
Centro de Neurociências e Biologia Celular (Coimbra, Portugal)
2009/12/02 Alpha-synuclein phosphorylation mediated by iron and rotenone in a cellular model of Parkinson's disease XL Reunião Anual da Sociedade Portuguesa de Farmacologia; Symposium: Neurodegenerative disorders
SPF (Porto, Portugal)
2009/06/26 Alpha-synuclein phosphorylation induced by iron and rotenone in a cellular model of Parkinson's disease 23ª Reunião do Grupo de Estudos do Envelhecimento e Demência
GEED (Luso, Portugal)
2008/07/12 Mitochondrial dysfunction and posttranslational modifications of alpha-synuclein 6th FENS Forum of European Neuroscience - Symposium Molecular Mechanisms in Parkinson's disease and other synucleinopathies
FENS (Genebra, Switzerland)

Supervision

Thesis Title
Role 		Degree Subject (Type)
Institution / Organization
2022/10/01 - 2026/09/30 Validation of the brain-targeting AAV-PHP.eB encoding the cholesterol hydroxylase CYP46A1 as a therapeutic strategy for spinocerebellar ataxia type-3 - towards the understanding of the neuroprotective mechanism
Co-supervisor
 Doctoral Program (PhD)
Centro de Inovação em Biomedicina e Biotecnologia (CIBB), Portugal

Universidade de Coimbra, Portugal
2022/01/01 - 2022/06/30 Intravenous Delivery of the brain-targeting AAV-PHP.eB encoding the cholesterol hydroxylase (CYP46A1) into a mouse model of spinocerebellar ataxia type 3: a promising non-invasive therapeutic strategy
Supervisor of Beatriz Machado Serra
Centro de Neurociências e Biologia Celular, Portugal

Universidade de Coimbra, Portugal
2021/10/01 - 2021/12/31 HealthyAgeing2020 - Work package 7 Ref.: CENTRO-01-0145-FEDER-000012 - Desenvolvimento de terapias génicas para o tratamento de doenças
Supervisor of Beatriz Machado Serra
Universidade de Coimbra, Portugal

Centro de Neurociências e Biologia Celular, Portugal
2020/11/01 - 2021/07/31 Intravenous delivery of the brain targeting PHP-eB encoding the cholesterol hydroxylase CYP46A1 into a mouse model of spinocerebellar ataxia type 3: a promising non-invasive therapeutic strategy
Supervisor of Beatriz Machado Serra
Centro de Neurociências e Biologia Celular, Portugal
2020/08/01 - 2020/10/31 Sobrexpressão mediada da CYP46A1 como estratégia terapêutica para doenças neurológicas
Supervisor of Beatriz Machado Serra
 Verão com Ciência: Metodologias de Investigação Científica – I&D – Terapia Génica e Células Estaminais (Specialization course)
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal

Centro de Neurociências e Biologia Celular, Portugal
2018/09 - 2019/10/07 Alpha-synuclein in extracellular vesicles - the spreading mechanism behind Parkinson's disease?
Supervisor of Andreia Raquel Minas Marques
 Mestrado em Biotecnologia Farmacêutica (Master)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2015/09 - 2016/09 Autophagy dysregulation in models of Machado-Joseph disease and Parkinson's disease
Co-supervisor of Chiara Soricelli
 Mestrado em Biologia Celular e Molecular (Master)
Universidade de Coimbra Departamento de Ciências da Vida, Portugal
2015/09 - 2016/06/15 Interplay between HIV and anti-retroviral therapy in neurologic impairments
Co-supervisor of Alexandra Raquel Patrício
 Mestrado Integrado em Medicina (Master)
Universidade de Coimbra Faculdade de Medicina, Portugal
2011/09 - 2013/07 Role of selective kinases in alpha-synuclein phosphorylation and its relevance in Parkinsons's disease
Co-supervisor of Paulo André Ribeiro dos Santos
 Mestrado em Biologia Celular e Molecular (Master)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2012/09 - 2013/06 Role of alpha-synuclein in neurodegeneration
Co-supervisor of Carolina Helena Freitas de Noronha
 Mestrado Integrado em Medicina (Master)
Universidade de Coimbra Faculdade de Medicina, Portugal

Event organisation

Event name
Type of event (Role) 		Institution / Organization
2023/01/11 - 2024/09 DCV.talks (2023/03/01)
Conference (Member of the Organising Committee)
 Universidade de Coimbra Departamento de Ciências da Vida, Portugal

Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2022/04/11 - 2022/04/14 Co-organization of the advanced course "Advanced Therapies"_PhD Programme in Experimental Biology and Biomedicine (PDBEB) (2022/04/11 - 2022/04/14) Centro de Neurociências e Biologia Celular, Portugal
2021/04/19 - 2021/04/23 Co-organization of the advanced course "Advanced Therapies" from the PhD Programme in Experimental Biology and Biomedicine (Programa Doutoral em Biologia Experimental e Biomedicina) (2021/04/19 - 2021/04/23)
Other (Co-organisor)
 Centro de Neurociências e Biologia Celular, Portugal
2016/06 - 2016/11/19 1st International Meeting Brain With [Out] Borders (2016/11/18 - 2016/11/19)
Congress (Co-organisor)
 Universidade de Coimbra, Portugal

Centro de Neurociências e Biologia Celular, Portugal

Event participation

Activity description
Type of event 		Event name
Institution / Organization
2022/01/13 - 2022/01/13 Women in Health - Workshop Series - objetivo de promover uma maior participação das mulheres em papéis de liderança no sector da saúde e debater os principais desafios para alcançar o mesmo.
Workshop
 How can a female leader shape the healthcare sector?
Universidade de Coimbra, Portugal

British Embassy Lisbon, Portugal
2021/12/14 - 2021/12/14 Women in Health - Workshop series - objetivo de promover uma maior participação das mulheres em papéis de liderança no sector da saúde e debater os principais desafios para alcançar o mesmo.
Workshop
 "Women in Health - How does Climate relate to (our) Health?"
Universidade de Coimbra, Portugal

British Embassy Lisbon, Portugal
2021/04/10 - 2021/04/11 Congresso Anual SPDMov 2021
Congress
 Doenças do Movimento
Sociedade Portuguesa de Doenças do Movimento, Portugal
2020/10/16 - 2020/10/17 Congresso anual SPDMov - 2020 (evento realizado online)
Congress
 Congresso SPDMov 2020 - Doença de Huntington
Sociedade Portuguesa de Doenças do Movimento, Portugal
2019/11/28 - 2019/11/28 Workshop "Knowledge Dissemination and Training of Young Scientists"
Workshop
 Knowledge Dissemination and Training of Young Scientists
EU Joint Programme Neurodegenerative Disease Research, Germany
2019/11/27 - 2019/11/27 Workshop "Patient and Public Involvement (PPI) in Research"
Workshop
 Patient and Public Involvement (PPI) in Research
EU Joint Programme Neurodegenerative Disease Research, Germany
2019/10/04 - 2019/10/04 Workshop "How to get published"
Workshop
 How to get Published
Elsevier Ltd, United Kingdom
2019/10/03 - 2019/10/03 Curso "First Aid Course"
Workshop
 First Aid Course
HIORSE, Portugal

Centro de Neurociências e Biologia Celular, Portugal
2019/09/26 - 2019/09/26 Workshop "AniBio Software"
Workshop
 AniBio Software
Centro de Neurociências e Biologia Celular, Portugal
2019/03/11 - 2019/03/17 Atividades de divulgação de ciência - Semana Internacional do Cérebro
Other
 Brain Awareness Week
Centro de Neurociências e Biologia Celular, Portugal
2018/03/23 - 2018/03/23 Workshop "Careers in Science and Beyond"
Workshop
 Overcoming challenges in scientific careers
Centro de Neurociências e Biologia Celular, Portugal
2018/03/12 - 2018/03/18 Divulgação de ciência
Other
 Brain Awareness Week
Centro de Neurociências e Biologia Celular, Portugal
2018/02/18 - 2018/02/18 Divulgação de ciência para público alvo (doentes de Machado Joseph e familiares)
Round table
 Doença de Machado-Joseph: do laboratório para a sociedade
Centro de Neurociências e Biologia Celular, Portugal
2016/10/20 - 2016/10/21 Workshop "Careers in Science and beyond"
Workshop
 Careers in Science and Beyond
Universidade do Porto Instituto de Investigação e Inovação em Saúde, Portugal
2016/09/16 - 2016/09/30 Gestão e Administração de ID Gestão e Administração de ID
Instituto Politécnico de Coimbra Instituto Superior de Contabilidade e Administração de Coimbra, Portugal
2016/08/15 - 2016/09/25 MOOC "Basics of Extracellular Vesicles"
Workshop
 Basics of Extracellular Vesicles
University of California Irvine, United States
2016/06/23 - 2016/06/24 Course "Biological markers in Neurological diseases - present and future approaches"
Workshop
 Biological markers in neurological diseases - present and future approaches
EU Joint Programme Neurodegenerative Disease Research, Germany

Centro de Neurociências e Biologia Celular, Portugal
2015/09/08 - 2015/09/08 Workshop "How to write great papers: from title to references, from submission to publication"
Workshop
 How to write great papers: from title to references, fom submission to publication
Universidade de Coimbra, Portugal
2015/07/16 - 2015/07/16 Workshop "Bioestatística para trabalhos de investigação" Bioestatística para trabalhos de investigação
Universidade de Coimbra Faculdade de Medicina, Portugal

Centro de Neurociências e Biologia Celular, Portugal
2015/03/14 - 2015/03/21 Divulgação de ciência
Other
 Brain Awareness Week
Centro de Neurociências e Biologia Celular, Portugal
2015/01/09 - 2015/01/09 Entrepreneurship activities for researchers
Workshop
 IPN CNC Business Idea Acceleration
Associação para a Inovação e Desenvolvimento em Ciência e Tecnologia, Portugal
2013/09/23 - 2013/10/04 Course in Laboratory Animal Science (FELASA, Category C)
Workshop
 Laboratory Animal Science
Universidade do Porto Instituto de Biologia Molecular e Celular, Portugal
2013/05/27 - 2013/05/27 Workshop "Grant Writing in Biosciences - New Challenges"
Workshop
 Grant Writing in Biosciences - New Challenges
Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
2009/12/15 - 2009/12/18 Course in "Science Communication"
Workshop
 Science Communication
Centro de Neurociências e Biologia Celular, Portugal
2009/09/02 - 2009/09/04 Course in "Multifluorescence Microscopy"
Workshop
 Multifluorescence Microscopy
Centro de Neurociências e Biologia Celular, Portugal

Carl Zeiss Jena GmbH, Germany
2007/01/22 - 2007/01/26 Course "Neurodegenerative disorders"
Workshop
 Neurodegenerative disorders
Centro de Neurociências e Biologia Celular, Portugal
2007/01/10 - 2007/01/12 Mini-course "Estatística Aplicada"
Workshop
 Estatística Aplicada
Centro de Neurociências e Biologia Celular, Portugal

Universidade de Évora Laboratório de Ciências do Mar, Portugal
2005/07/05 - 2005/07/10 Summer School "Neuro-glia interaction in aging and disease"
Workshop
 Summer School FENS/BLACKWELL "Neuro-glia interaction in aging and disease"
Centro de Neurociências e Biologia Celular, Portugal

Jury of academic degree

Topic
Role 		Candidate name (Type of degree)
Institution / Organization
2023/04/11 Assessing the Role of Nucleocytoplasmic Transport Mechanisms in Machado-Joseph Disease Pathogenesis
(Thesis) Arguer
 Maria Inês Morgado Oliveira Martins (PhD)
Universidade de Coimbra, Portugal

Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
2021/11/18 Optimization of the recovery of extracellular vesicles from filamentous fungi
(Thesis) Main arguer
 Rita Sofia de Jesus Pita Domingues (Master)
Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2021/07/22 Machado-Joseph Disease Comorbidities and Medication - a large cohort analysis.
(Thesis) Main arguer
 Marina Albino Costa (Master)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2020/11/16 Mitochondrial Dynamics and mitophagy in cellular models of Parkinson's disease
(Thesis) Main arguer
 Bruno Miguel Sequeira dos Santos (Master)
Universidade de Coimbra, Portugal

Universidade de Coimbra Faculdade de Ciencias e Tecnologia, Portugal
2019/10/07 Alpha-synuclein in exosomes: the spreading mechanism behind Parkinson's disease?
Supervisor
 Andreia Raquel Minas Marques (Master)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2019/07/18 Biotecnologia Aplicada à Doença de Parkinson: Estratégias de Terapia Génica
(Thesis) Main arguer
 André Filipe dos Santos Ribeiro (Master)
Universidade de Coimbra Faculdade de Farmácia, Portugal
2016/06/15 Interplay between HIV and antiretroviral therapy in the progression of neurological disorders
Thesis Member
 Alexandra Raquel Patrício (Master)
Universidade de Coimbra Faculdade de Medicina, Portugal

Association member

Society Organization name Role
2016 - Current International Society for Extracellular Vesicles (ISEV) Sócio
2006 - Current Sociedade Portuguesa de Neurociências (SPN) Sócio
2010/10 - 2012/02 ACREDITAR - Associação de Pais e Amigos de Crianças com Cancro Voluntária

Consulting

Activity description Institution / Organization
2019/12/15 - 2020/01/05 Prestação de serviços por convite da empresa OWLPHARMA para elaboração do seguinte documento: "THE COMMON TECHNICAL DOCUMENT FOR THE REGISTRATION OF PHARMACEUTICALS FOR HUMAN USE; MODULE 2.4 - NON-CLINICAL OVERVIEW WARFARIM SODIUM [HIKMA], 1 MG/ML, ORAL SUSPENSION (WARFARIN)" OWLPHARMA, Portugal

Course / Discipline taught

Academic session Degree Subject (Type) Institution / Organization
2023/04/22 - 2023/04/22 Targeting brain cholesterol metabolism as a therapeutic strategy for neurodegenerative disorders Módulo VIII: TERAPÊUTICA MOLECULAR E ENGENHARIA DE TECIDOS (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2022/04/12 - 2022/04/12 Brain Cholesterol Deregulation in Neurodegenerative Disorders - new avenues for therapeutic approaches. Focusing on Machado-Joseph Disease. Advanced Therapies (Doutoramento) Centro de Neurociências e Biologia Celular, Portugal
2021/04/21 - 2021/04/21 Intravenous delivery of the brain-targeting AAV-PHPeB encoding CYP46A1 to overcome brain cholesterol deregulation in neurodegenerative disorders - focusing on Machado Joseph disease Advanced Therapies - BEB PhD program (Doutoramento) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2020/10/07 - 2020/10/07 Gene delivery therapeutic strategies using adeno associated viral vectors encoding CYP46A1 into mouse models of Machado Joseph Disease (SCA3) Curso de Verão Metodologias de Investigação Científicas (Especialização pós-licenciatura) Universidade de Coimbra Instituto de Investigação Interdisciplinar, Portugal
2020/05/02 - 2020/05/02 Stem cells and exosomes: a bi-directional intercommunication. Módulo 8: Terapêutica Molecular e Engenharia de Tecidos (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2019/04/22 - 2019/04/22 Stem Cells and exosomes: a bi-directional intercommunication Advanced Therapies - BEB PhD Program (Doutoramento) Centro de Neurociências e Biologia Celular, Portugal
2019/04/13 - 2019/04/13 Induced Pluripotent Stem Cells: exosomes Terapêutica Molecular e Engenharia de Tecidos - Mestrado em Biotecnologia Farmacêutica (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2018/05/07 - 2018/05/07 Stem cells and exosomes: a bi-directional intercommunication Advanced Therapies, BEB PhD Program (Curso de doutoramento (conclusão de unidades curriculares)) Centro de Neurociências e Biologia Celular, Portugal
2018/04/21 - 2018/04/21 Induced Pluripotent Stem Cells: exosomes Terapêutica Molecular e Engenharia de Tecidos - Mestrado em Biotecnologia Farmacêutica (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2018/01/11 - 2018/01/11 Doença de Parkinson: diferentes mecanismos, a mesma (dis)função Licenciatura em Ciências Biomédicas (Licenciatura) Universidade Católica Portuguesa Instituto de Ciências da Saúde Viseu, Portugal
2017/06/09 - 2017/06/09 Exossomas: de biomarcadores a sistemas terapêuticos Mestrado em Farmacologia Aplicada (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal
2017/04/07 - 2017/04/07 Induced Pluripotent Stem Cells: exosomes Mestrado em Biotecnologia Farmacêutica - Terapêutica Molecular e Engenharia de Tecidos (Mestrado) Universidade de Coimbra Faculdade de Farmácia, Portugal

Evaluation committee

Activity description
Role 		Institution / Organization Funding entity
2021/08/06 - 2021/08/19 Atribuição de uma bolsa de investigação, no âmbito do projeto HEALTHYAGING 2020, com a referência CENTRO-01-0145-FEDER-000012 e com o título HealtyAging2020 - CENTRO-01-0145-FEDER-000012, financiado através do Fundo Europeu de Desenvolvimento Regional (FEDER) da União Europeia, através do Programa Operacional Regional do Centro (CENTRO2020) do Portugal 2020, com as seguintes características: Área(s) Científica(s): Ciências da Vida ou da Saúde, Ciências Farmacêuticas e áreas afins.
Evaluator
 Universidade de Coimbra, Portugal

Centro de Neurociências e Biologia Celular, Portugal 		Universidade de Coimbra

Interview (tv / radio show)

Program Topic
2021/07/15 - 2021/07/31 "A Saúde no Saber - literacia em saúde" A Sáude no Saber - Doenças Raras
2020/09/25 - 2020/09/25 Rubric Selfie Science - 24th epidose Doença de Machado Joseph - impacto da desregulação do colesterol do cérebro nesta e em outras doenças neurodegenerativas

Mentoring / Tutoring

Topic Student name
2017/02 - 2017/04 Lab Rotation - Role and Mechanisms of alpha-synuclein spreading in Parkinson's disease Fábio Duarte
2011/05 - 2012/06 Lab Rotation - Alpha-synuclein and Parkinson's disease Miguel Fernando Alves Ferreira
2009/09 - 2012/06 Traineeship - Analysis of GDNF protection in a cellular model of Parkinson´s disease Carolina Helena Freitas de Noronha
2011/04 - 2011/07 Traineeship - Role of GDNF on alpha-synuclein phosphorylation and apoptotic features in a cellular model of Parkinson's disease Francisco Espinosa Aranda
2011/05 - 2011/06 Lab rotation - Alpha-synuclein and Parkinson's disease Ana Isabel Nunes Farinha
2011/03 - 2011/04 Lab Rotation - Alpha-synuclein and Parkinson's disease Ricardo Alexandre Gomes Leitão
2011/03 - 2011/04 Lab Rotation - Alpha-synuclein and Parkinson's disease Sónia Maria Costa André
2010/06 - 2010/07 Lab Rotation (PhD doctoral program) - Alpha-synuclein and Parkinson's disease Vitor Hugo Dias Martins
2007/05 - 2007/07 Laboratory Traineeship - Parkinson's disease and alpha-synuclein Rui Pedro Bento

Other jury / evaluation

Activity description Institution / Organization
2021/05/07 - 2021/07/21 Membro de Júri do Procedimento Público de Aquisição com a referência 21J00051. Aquisição de equipamentos de laboratório para a Universidade de Coimbra. Universidade de Coimbra, Portugal
2020/05/21 - 2020/05/25 Membro de Júri do Procedimento Público de Aquisição com a referência 20A00004. Aquisição de um sistema compacto automatizado de microscopia de fluorescência Universidade de Coimbra, Portugal